Publications by authors named "Dong Chen"

1,883 Publications

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MiR-542-3p Suppresses Neuroblastoma Cell Proliferation and Invasion by Downregulation of KDM1A and ZNF346.

Open Life Sci 2020 10;15:173-184. Epub 2020 Apr 10.

Department II of General Surgery, Xi'an Children's Hospital, Xi'an, Shaanxi, China.

Neuroblastoma is one of the most common malignancies in infants and children. MicroRNAs (miRNAs) have been reported as significant regulators that play important roles in neuroblastoma development. This research aimed to analyze the functional mechanism of miR-542-3p in neuroblastoma. Here, we found that miR-542-3p was downregulated and KDM1A as well as ZNF346 were upregulated in neuroblastoma tissues and cells. Both overexpression of miR-542-3p and the knockdown of KDM1A suppressed cell proliferation and invasion in neuroblastomas. Moreover, miR-542-3p reduced the levels of KDM1A and ZNF346 through interaction. Both KDM1A overexpression and ZNF346 upregulation weakened the effect of miR-542-3p on neuroblastoma cells. Besides, miR-542-3p negatively regulated tumor growth . Our results suggested that miR-542-3p suppressed cell proliferation and invasion by targeting KDM1A and ZNF346 in neuroblastomas, providing a theoretical basis for the treatment of neuroblastoma.
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http://dx.doi.org/10.1515/biol-2020-0018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8114778PMC
April 2020

Pathologic Spectrum and Molecular Landscape of Myeloid Disorders Harboring SF3B1 Mutations.

Am J Clin Pathol 2021 May 12. Epub 2021 May 12.

Division of Hematopathology, Mayo Clinic College of Medicine, Rochester, MN, USA.

Objectives: SF3B1 mutations are the most common mutations in myelodysplastic syndromes (MDS). The International Working Group for the Prognosis of MDS (IWG-PM) recently proposed SF3B1-mutant MDS (SF3B1-mut-MDS) as a distinct disease subtype. We evaluated the spectrum and molecular landscape of SF3B1-mutated myeloid disorders and assessed the prognostication in MDS harboring SF3B1 mutations (MDS-SF3B1).

Methods: Cases were selected by retrospective review. Clinical course and laboratory and clinical findings were collected by chart review. SF3B1-mut-MDS was classified following IWG-PM criteria.

Results: SF3B1 mutations were identified in 75 of 955 patients, encompassing a full spectrum of myeloid disorders. In MDS-SF3B1, Revised International Prognostic Scoring System (IPSS-R) score greater than 3 and transcription factor (TF) comutations were adverse prognostic markers by both univariate and multivariate analyses. We confirmed the favorable outcome of IWG-PM-defined SF3B1-mut-MDS. Interestingly, it did not show sharp prognostic differentiation within MDS-SF3B1.

Conclusions: SF3B1 mutations occur in the full spectrum of myeloid disorders. We independently validated the favorable prognostication of IWG-PM-defined SF3B1-mut-MDS. However it may not provide sharp prognostication within MDS-SF3B1 where IPSS-R and TF comutations were prognostic-informative. Larger cohort studies are warranted to verify these findings and refine MDS-SF3B1 prognostication.
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http://dx.doi.org/10.1093/ajcp/aqab010DOI Listing
May 2021

Boosting Reversibility of Mn-Based Tunnel-Structured Cathode Materials for Sodium-Ion Batteries by Magnesium Substitution.

Adv Sci (Weinh) 2021 May 18;8(9):2004448. Epub 2021 Feb 18.

Department of Materials Science Fudan University Shanghai 200433 P. R. China.

Electrochemical irreversibility and sluggish mobility of Na in the cathode materials result in poor cycle stability and rate capability for sodium-ion batteries. Herein, a new strategy of introducing Mg ions into the hinging sites of Mn-based tunnel-structured cathode material is designed. Highly reversible electrochemical reaction and phase transition in this cathode are realized. The resulted NaMnMgO with Mg in the hinging Mn-O square pyramidal exhibits promising cycle stability and rate capability. At a current density of 2 C, 67% of the initial discharge capacity is retained after 800 cycles (70% at 20 C), much improved than the undoped NaMnO. The improvement is attribute to the enhanced Na diffusion kinetics and the lowered desodiation energy after Mg doping. Highly reversible charge compensation and structure evolution are proved by synchrotron-based X-ray techniques. Differential charge density and atom population analysis of the average electron number of Mn indicate that NaMnMgO is more electron-abundant in Mn 3d orbits near the Fermi level than that in NaMnO, leading to higher redox participation of Mn ions.
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http://dx.doi.org/10.1002/advs.202004448DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8097362PMC
May 2021

Potential Mechanisms Mediating the Protective Effects of -Derived Peptides in Mitigating DSS-Induced Colitis.

J Agric Food Chem 2021 May 6. Epub 2021 May 6.

National Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, P. R. China.

Intestinal barrier dysfunction and inflammatory cytokine secretion play crucial roles in inflammatory bowel disease (IBD). Herein, we investigated the protective effects of -derived peptides SDIKHFPF and SDLKHFPF against dextran sulfate sodium-induced colitis. Both peptides alleviated colitis signs, including diarrhea, weight loss, bloody stools, colon shortening, and histopathological changes, while reducing mucus destruction, goblet cell exhaustion, and intestinal permeability. SDIKHFPF and SDLKHFPF protected the barrier function by promoting the expression of tight junction (TJ) zonula occludens-1 and occludin within the colon, as well as attenuating colonic inflammation through myeloperoxidase and pro-inflammatory cytokine suppression. Western blotting indicated that the peptides suppressed myosin light chain kinase (MLCK) and nuclear factor kappa B (NF-κB) levels, inhibiting MLC phosphorylation. SDLKHFPF was more potent than SDIKHFPF. These findings suggest that peptide SDLKHFPF mitigates colitis by regulating TJ protein expression and pro-inflammatory cytokine production via NF-κB/MLCK/p-MLC signaling, improving the barrier function.
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http://dx.doi.org/10.1021/acs.jafc.1c01908DOI Listing
May 2021

Safety and Efficacy of Topical Administration of Tranexamic Acid in High-Risk Patients Undergoing Posterior Lumbar Interbody Fusion Surgery.

World Neurosurg 2021 Apr 30. Epub 2021 Apr 30.

Department of Orthopedics, Clinical Medical College of Yangzhou University, Yangzhou 225001, China. Electronic address:

Objective: To evaluate the safety and efficacy of topical administration of tranexamic acid (TXA) in high-risk patients undergoing posterior lumbar interbody fusion (PLIF) surgery METHODS: In this single-center, retrospective cohort study, a total of 120 patients with lumbar degenerative disease who had a previous history of cardiovascular or cerebrovascular embolism and who underwent single-level posterior lumbar interbody fusion (PLIF) surgery between December 2018 and December 2019 were included and allocated to two groups according to whether they had been administered TXA. In the TXA group (n = 60), the wound surface was topically soaked with TXA (1 g in 100 mL of saline solution) for 5 minutes before wound closure. In the control group (n = 60), the wound surface was topically soaked with the same volume of normal saline. SPSS software, version 26.0, was employed to analyze demographics, including surgical traits, blood loss, drainage, length of hospital stays (LOS), blood biochemical indices, prethrombotic state molecular markers, coagulation function and adverse events.

Results: Total blood loss (TBL), visible blood loss (VBL), postoperative drainage, removal time of drainage tube and LOS were significantly lower in the TXA group than in the control group. However, there was no significant difference between the two groups in hidden blood loss (HBL), hepatorenal function, coagulation function, prethrombotic state molecular markers, transfusion rate or complications during the perioperative period.

Conclusions: In single-level PLIF surgery, topical administration of TXA could significantly reduce TBL, VBL, postoperative drainage, removal time of drainage tube, and LOS without increasing the risk of thromboembolic events in high-risk patients with prior histories of thrombosis.
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http://dx.doi.org/10.1016/j.wneu.2021.04.088DOI Listing
April 2021

The Use of Deep Learning and VR Technology in Film and Television Production From the Perspective of Audience Psychology.

Front Psychol 2021 18;12:634993. Epub 2021 Mar 18.

College of Business, Gachon University, Seoul, South Korea.

As the development of artificial intelligence (AI) technology, the deep-learning (DL)-based Virtual Reality (VR) technology, and DL technology are applied in human-computer interaction (HCI), and their impacts on modern film and TV works production and audience psychology are analyzed. In film and TV production, audiences have a higher demand for the verisimilitude and immersion of the works, especially in film production. Based on this, a 2D image recognition system for human body motions and a 3D recognition system for human body motions based on the convolutional neural network (CNN) algorithm of DL are proposed, and an analysis framework is established. The proposed systems are simulated on practical and professional datasets, respectively. The results show that the algorithm's computing performance in 2D image recognition is 7-9 times higher than that of the Open Pose method. It runs at 44.3 ms in 3D motion recognition, significantly lower than the Open Pose method's 794.5 and 138.7 ms. Although the detection accuracy has dropped by 2.4%, it is more efficient and convenient without limitations of scenarios in practical applications. The AI-based VR and DL enriches and expands the role and application of computer graphics in film and TV production using HCI technology theoretically and practically.
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http://dx.doi.org/10.3389/fpsyg.2021.634993DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8080441PMC
March 2021

Portable bacterial CRISPR transcriptional activation enables metabolic engineering in Pseudomonas putida.

Metab Eng 2021 Apr 27. Epub 2021 Apr 27.

Molecular Engineering & Sciences Institute and Center for Synthetic Biology,University of Washington,Seattle, WA, 98195, USA; Department of Chemistry,University of Washington,Seattle, WA, 98195, USA. Electronic address:

CRISPR-Cas transcriptional programming in bacteria is an emerging tool to regulate gene expression for metabolic pathway engineering. Here we implement CRISPR-Cas transcriptional activation (CRISPRa) in P. putida using a system previously developed in E. coli. We provide a methodology to transfer CRISPRa to a new host by first optimizing expression levels for the CRISPRa system components, and then applying rules for effective CRISPRa based on a systematic characterization of promoter features. Using this optimized system, we regulate biosynthesis in the biopterin and mevalonate pathways. We demonstrate that multiple genes can be activated simultaneously by targeting multiple promoters or by targeting a single promoter in a multi-gene operon. This work will enable new metabolic engineering strategies in P. putida and pave the way for CRISPR-Cas transcriptional programming in other bacterial species.
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http://dx.doi.org/10.1016/j.ymben.2021.04.002DOI Listing
April 2021

The pH-dependent degradation of sulfadiazine using natural siderite activating PDS: The role of singlet oxygen.

Sci Total Environ 2021 Apr 16;784:147117. Epub 2021 Apr 16.

Key Laboratory of Nano-minerals and Pollution Control of Anhui Higher Education Institutes, Hefei University of Technology, Hefei 230009, China; Institute of Environmental Minerals and Materials, School of Resources and Environmental Engineering, Hefei University of Technology, Hefei 230009, China.

Occurring naturally siderite (FeCO) was used as the heterogeneous catalyst to activate peroxodisulfate (PDS) for the degradation of sulfadiazine under different initial pH values. The findings of this system exhibited various ROS (e.g. O, SO and OH) present during a wide range of pH values. Among them, O could significantly facilitate the initial degradation rate, and the increased pH enhanced the role of O. The factors including initial pH values, siderite dosage, PDS concentration, initial contaminants concentration, and water matrix were discussed. The role of each ROS was investigated through quenching test and electron paramagnetic resonance (EPR). Furthermore, the comprehensive degradation process was proposed based on the LC-MS results. And the cycle test demonstrates the reusability of siderite at a pH of 3. Accordingly, this study is of great significance for understanding the degradation of such sulfonamide pollutants in the siderite/PDS system.
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http://dx.doi.org/10.1016/j.scitotenv.2021.147117DOI Listing
April 2021

The beneficial effects of ultraviolet light supplementation on bone density are associated with the intestinal flora in rats.

Appl Microbiol Biotechnol 2021 May 24;105(9):3705-3715. Epub 2021 Apr 24.

Key Laboratory for Biomechanics and Mechanobiology of the Ministry of Education, Beijing Advanced Innovation Centre for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang University, Beijing, 100191, China.

The general public spends one-third of its time under artificial lighting, which lacks bands beneficial to human health, and long-term exposure will have a negative impact on bone health. Here, we report the effects of long-term, low-dose ultraviolet (UV) supplementation to white light-emitting diode (LED) light exposure on intestinal microorganisms and bone metabolism, as well as the correlations between the two. Normal and ovariectomized rats were irradiated with LED white light with or without supplementation with UV. The effects of UV supplementation on the intestinal flora and the relationship between the intestinal flora and bone were investigated by measuring the intestinal flora, bone metabolism markers, and bone histomorphology. UV supplementation affected the bone density and bone mass by changing the relative content of Firmicutes, Saccharibacteria, and Proteobacteria; however, the intestinal flora were not the only factors affecting bone. Ultraviolet supplementation changed the composition and function of the gut flora in the bone loss model. By increasing the synthesis of short-chain fatty acids and affecting immunomodulatory, intestinal flora directly or indirectly regulate the activity of osteoclasts and thus mediate UV-mediated improvements in bone metabolism. Our work shows that UV supplementation affects bone density by influencing the intestinal flora, introducing a novel strategy to develop healthier artificial light sources and prevent bone loss. KEY POINTS: • We measured the bone metabolism markers and bone histomorphometry of rats. • The diversity, composition, and function of intestinal flora were analyzed. • The relationship between gut microbiota and host bone physiology was analyzed.
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http://dx.doi.org/10.1007/s00253-021-11282-2DOI Listing
May 2021

COVID-19 may increase the risk of insulin resistance in adult patients without diabetes: A 6-month prospective study.

Endocr Pract 2021 Apr 19. Epub 2021 Apr 19.

Department of Endocrinology and Metabolism, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai, China. Electronic address:

Objective: During the coronavirus disease 2019 (COVID-19) pandemic, exploring insulin resistance and β-cell activity is important for understanding COVID-19-associated new-onset diabetes. This study aimed to assess insulin sensitivity and fasting insulin secretion in COVID-19 patients without diabetes on admission and at 3 and 6 months after discharge.

Methods: This 6-month prospective study assessed data from the records of 64 patients without diabetes diagnosed with COVID-19 at Wenzhou Central Hospital, China. Each patient was followed up for 3 and 6 months after discharge. Repeated measures analysis of variance was used to investigate differences in multiple measurements of the same variable at different times. Linear regression analysis was performed to analyze the contributor for changes in triglyceride-glucose (TyG) index.

Results: Fasting C-peptide levels in patients at baseline were lower than the normal range (1.1-4.4 μg/L). Compared with baseline, patients had significantly elevated fasting C-peptide levels (0.35±0.24 vs. 2.36±0.98 vs. 2.52±1.11 μg/L, P˂0.001), HOMA-CP (0.42, IQR 0.36-0.62 vs. 2.54, IQR 1.95-3.42 vs. 2.90, IQR 2.02-4.23, P˂0.001) and TyG indexes (8.57±0.47 vs. 8.73±0.60 vs. 8.82±0.62, P=0.006), and decreased fasting glucose levels (5.84±1.21 vs. 4.95±0.76 vs. 5.40±0.68 mmol/L, P=0.003) during the 3 and 6-month follow-up. Male gender [β (95%CI): -0.312 (-0.590, -0.034)], age [0.012 (0.001, 0.023)], interferon-alfa treatment during hospitalization [0.540 (0.029, 1.051)], and changes in TC [0.217 (0.069, 0.366)] and HDL [-0.477 (-0.881, -0.074)] levels were significantly associated with changes in the TyG index.

Conclusions: Our study provided the first evidence that COVID-19 may increase the risk of insulin resistance in patients without diabetes.
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http://dx.doi.org/10.1016/j.eprac.2021.04.004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8054613PMC
April 2021

Perioperative Hidden Blood Loss in Elderly Cervical Spondylosis Patients With Anterior Cervical Discectomy Fusion and Influencing Factors.

Geriatr Orthop Surg Rehabil 2021 31;12:21514593211002164. Epub 2021 Mar 31.

Department of Orthopedics, Clinical Medical College of Yangzhou University, Yangzhou, China.

Introduction: To analyze the perioperative hidden blood loss (HBL) and its influencing factors in elderly cervical spondylosis patients treated with anterior cervical discectomy fusion (ACDF).

Materials And Methods: From January 2017 to December 2018, 128 elderly cervical spondylosis patients (age > 65 y) treated with ACDF were selected. The patients' height, weight, duration of symptoms, previous medical history and other basic information were routinely recorded. The hemoglobin (Hb), hematocrit (Hct) and blood coagulation function preoperative and the next day postoperative were recorded. The operation time, surgical bleeding, ASA classification, fixation method, total drainage and the time for extraction of drainage tube were recorded. The total blood loss (TBL) was calculated according to the Gross's formula, and HBL was calculated based on TBL, total drainage and surgical bleeding. The statistical analysis of HBL was performed, and then influential factors were further analyzed by multivariate linear regression analysis and t test.

Results: The mean surgical bleeding was 102.70 ± 46.78 mL and HBL was 487.98 ± 255.96 mL. HBL accounted for 67.61 ± 5.20% of TBL. According to the multiple linear regression analysis, the gender (P = 0.047), operation time (P = 0.000), fixation method (P = 0.014) and international normalized ratio (INR) (P = 0.003) influenced the amount of HBL. Body mass index (BMI) (P = 0.624), hypertension (P = 0.977), diabetes (P = 0.528), blood type (P = 0.577), ASA classification (P = 0.711), duration of symptoms (P = 0.661), preoperative cobb angle (P = 0.152), number of surgical level (P = 0.709), intramedullary hyperintensity (P = 0.967), drainage time (P = 0.294), postoperative drainage volume (P = 0.599), prothrombin time (PT) (P = 0.674), activated partial thromboplastin time (APTT) (P = 0.544) and thrombin time (TT) (P = 0.680) had no correlation with the amount of HBL.

Conclusions: There was obvious HBL during the perioperative period of ACDF in elderly cervical spondylosis patients. The male patients, longer operation time, fusion with titanium plate and cage and high INR were independent risk factors for HBL.
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http://dx.doi.org/10.1177/21514593211002164DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8020224PMC
March 2021

Treating Lumbar Fracture Using the Mixed Reality Technique.

Biomed Res Int 2021 29;2021:6620746. Epub 2021 Mar 29.

Department of Spine Surgery, Wuhan Puren Hospital, Wuhan University of Science and Technology, Benxi Rd. 1#, Wuhan, 430033 Hubei, China.

The mixed reality (MR) technique has recently been widely used in the orthopedic surgery with satisfactory results reported. However, few studies have focused on the application of MR in the Lumbar fracture (LF). In this retrospective study, our aim is to analyze some findings by investigating the feasibility of MR applied to lumbar fracture treatment. Posterior vertebrectomy has been operated on 7 patients. The MR-based intraoperative three-dimensional image-guided navigation system (MITINS) was used to assist implantation of pedicle screws. The feasibility and safety of pedicle screw implantation were assessed by postsurgery radiography. The visual analog scale (VAS) and Oswestry Disability Index (ODI) were used to assess the pain level and recovery situation before and after surgery. 57 pedicle screws were safely and precisely placed into three-dimensional lumbar models by using MITINS. No screw was found outside the pedicle of the models, and it was not necessary for the X-ray to provide extra locative information during the operation with the use of MITINS. In summary, the application of MITINS is feasible, safe, and accurate while the lumbar fracture surgery is processing, providing satisfactory assistance for spine surgeons.
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http://dx.doi.org/10.1155/2021/6620746DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8024068PMC
March 2021

Interleukin-17D regulates group 3 innate lymphoid cell function through its receptor CD93.

Immunity 2021 Apr;54(4):673-686.e4

Institute for Immunology and School of Medicine, Tsinghua University, Beijing 100084, China; Shanghai Jiao Tong University School of Medicine Affiliated Renji Hospital, Shanghai 200127, China. Electronic address:

The interleukin (IL)-17 family, consisting of six members, promotes host defense but can in some context promote the development of autoimmune disease. Here, we examined the role of IL-17D, a poorly understood member in the IL-17 family. IL-17D was expressed primarily by colonic epithelial cells. Il17d mice were more susceptible to acute colitis, bacterial infection and experimentally induced colon cancer than their wildtype counterparts. Il17d deficiency impaired IL-22 production by group 3 innate lymphoid cells (ILC3s) and reduced expression of IL-22-dependent antimicrobial peptides, RegIIIβ and RegIIIγ, in colon tissue at steady state and in colitis; this was associated with changes in microbial composition and dysbiosis. Protein purification studies revealed that IL-17D bound not canonical IL-17 receptors, but rather CD93, a glycoprotein expressed on mature ILC3s. Mice lacking Cd93 in ILC3s exhibited impaired IL-22 production and aggravated colonic inflammation in experimental colitis. Thus, an IL-17D-CD93 axis regulates ILC3 function to preserve intestinal homeostasis.
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http://dx.doi.org/10.1016/j.immuni.2021.03.018DOI Listing
April 2021

Clinical characteristics and plasma antibody titer of patients with COVID-19 in Zhejiang, China.

J Zhejiang Univ Sci B 2020 Dec.;21(12):955-960

Research Center of Blood Transfusion Medicine, Ministry of Education Key Laboratory of Laboratory Medicine, Department of Blood Transfusion, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou 310014, China.

Coronavirus disease 2019 (COVID-19) is an infectious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which first affected humans in China on December 31, 2019 (Shi et al., 2020). Coronaviruses generally cause mild, self-limiting upper respiratory tract infections in humans, such as the common cold, pneumonia, and gastroenteritis (To et al., 2013; Berry et al., 2015; Chan et al., 2015). According to the Report of the World Health Organization (WHO)-China Joint Mission on COVID-19 (WHO, 2020), the case fatality rate of COVID-19 increases with age, while the rate among males is higher than that among females (4.7% and 2.8%, respectively). Since an effective vaccine and specific anti-viral drugs are still under development, passive immunization using the convalescent plasma (CP) of recovered COVID-19 donors may offer a suitable therapeutic strategy for severely ill patients in the meantime. So far, several studies have shown therapeutic efficacy of CP transfusion in treating COVID-19 cases. A pilot study first reported that transfusion of CP with neutralizing antibody titers above 1:640 was well tolerated and could potentially improve clinical outcomes through neutralizing viremia in severe COVID-19 cases (Chen et al., 2020). Immunoglobulin G (IgG) and IgM are the most abundant and important antibodies in protecting the human body from viral attack (Arabi et al., 2015; Marano et al., 2016). Our study aimed to understand the aspects of plasma antibody titer levels in convalescent patients, as well as assessing the clinical characteristics of normal, severely ill, and critically ill patients, and thus provide a basis for guiding CP therapy. We also hoped to find indicators which could serve as a reference in predicting the progression of the disease.
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http://dx.doi.org/10.1631/jzus.B2000593DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7759456PMC
April 2021

Metabolomics reveals sex-specific metabolic shifts and predicts the duration from positive to negative in non-severe COVID-19 patients during recovery process.

Comput Struct Biotechnol J 2021 6;19:1863-1873. Epub 2021 Apr 6.

Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325015, China.

Metabolic profiling in COVID-19 patients has been associated with disease severity, but there is no report on sex-specific metabolic changes in discharged survivors. Herein we used an integrated approach of LC-MS-and GC-MS-based untargeted metabolomics to analyze plasma metabolic characteristics in men and women with non-severe COVID-19 at both acute period and 30 days after discharge. The results demonstrate that metabolic alterations in plasma of COVID-19 patients during the recovery and rehabilitation process were presented in a sex specific manner. Overall, the levels of most metabolites were increased in COVID-19 patients after the cure relative to acute period. The major plasma metabolic changes were identified including fatty acids in men and glycerophosphocholines and carbohydrates in women. In addition, we found that women had shorter length of hospitalization than men and metabolic characteristics may contribute to predict the duration from positive to negative in non-severe COVID-19 patients. Collectively, this study shed light on sex-specific metabolic shifts in non-severe COVID-19 patients during the recovery process, suggesting a sex bias in prognostic and therapeutic evaluations based on metabolic profiling.
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http://dx.doi.org/10.1016/j.csbj.2021.03.039DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8021501PMC
April 2021

Genetic Variants in and Are Associated With the Risk of HCV Infection Among Chinese High-Risk Population.

Front Genet 2021 25;12:630310. Epub 2021 Mar 25.

Department of Infectious Diseases, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Background: The tumor necrosis factor superfamily () and TNF receptor superfamily () play important roles in the immune responses to infections. The aim of this study was to determine the impact of single nucleotide polymorphisms (SNPs) of several genes on the risk of hepatitis C virus (HCV) infection in the Chinese high-risk population.

Methods: The -rs1234313, -rs7514229, -rs3181366, -rs2295800, -rs2298209, and -rs2230625 SNPs were genotyped in 2309 uninfected controls, 597 subjects with spontaneous HCV clearance and 784 patients with persistent HCV infection using the TaqMan-MGB assay. The putative functions of the positive SNPs were determined using online bioinformatics tools.

Results: After adjusting for gender, age, high-risk population, alanine transaminase (ALT), aspartate aminotransferase (AST), -rs12979860 and rs8099917 genotypes, the non-conditional logistic regression showed that rs7514229-T, rs3181366-T, and rs2295800-C were associated with an increased risk of HCV infection (all < 0.05). Combined analysis of rs7514229-T and rs3181366-T risk alleles showed that the subjects carrying 2-4 risk alleles were more susceptible to HCV infection compared with those lacking any risk allele (all < 0.001). Furthermore, the risk of HCV infection increased with the number of risk alleles ( < 0.001). analysis showed that rs7514229, rs3181366, and rs2295800 polymorphisms may affect the transcription of mRNA by regulating miRNA binding, TF binding, and promoter activation, respectively, which may have biological consequences.

Conclusion: -rs7514229, -rs3181366, and -rs2295800 are associated with increased risk of HCV infection in the Chinese high-risk population.
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http://dx.doi.org/10.3389/fgene.2021.630310DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8027328PMC
March 2021

Novel High-Efficiency Polymer Acceptors via Random Ternary Copolymerization Engineering Enables All-Polymer Solar Cells with Excellent Performance and Stability.

ACS Appl Mater Interfaces 2021 Apr 9;13(15):17892-17901. Epub 2021 Apr 9.

College of Chemistry/Institute of Polymers and Energy Chemistry (IPEC), Nanchang University, Nanchang 330031, P. R. China.

Continuous breakthroughs have been achieved in improving the efficiency of all-polymer solar cells (all-PSCs) using diimide-based polymer acceptors, and their easy-to-synthesize, low-cost, and high stability attributes make them potential candidates for use in commercial all-PSCs. However, their low light absorption coefficient, strong aggregation, and poor adaptability with high-efficient polymer donors still limit further improvements in the device performance. Here, we combine the advantages of fluorinated bithiophene and rhodanine dye molecules to create low-cost diimide-based polymer acceptors, PNDI-2FT-TR10 and PNDI-2FT-TR20, by random copolymerization for achieving highly efficient and stable all-PSCs. The synergistic effects of fluorine atoms and rhodanine dye molecules not only significantly improve the absorption coefficient but also enable enhanced miscibility and stability of the blend film. When blended with a PM6 donor, the PNDI-2FT-TR10-based device exhibits a notable power conversion efficiency (PCE) of 10.71% with a short-circuit current () of 17.32 mA cm. Note that both the PCE and show outstanding values for diimide-based all-PSCs, and this is the first report on blending diimide-based polymer acceptors with the PM6 donor to achieve high-performance all-PSCs. Moreover, the favorable morphology of the active layer enables the device to have good thickness tolerance and thermal stability. The results demonstrate that the absorption coefficients, blend morphology, and photovoltaic properties of all-PSCs could be rationally optimized by a random copolymer.
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http://dx.doi.org/10.1021/acsami.1c03739DOI Listing
April 2021

A multi-channel collagen scaffold loaded with neural stem cells for the repair of spinal cord injury.

Neural Regen Res 2021 Nov;16(11):2284-2292

Clinical Stem Cell Center, Nanjing Drum Tower Hospital, the Affiliated Hospital of Nanjing University Medical School, Nanjing, Jiangsu Province, China.

Collagen scaffolds possess a three-dimensional porous structure that provides sufficient space for cell growth and proliferation, the passage of nutrients and oxygen, and the discharge of metabolites. In this study, a porous collagen scaffold with axially-aligned luminal conduits was prepared. In vitro biocompatibility analysis of the collagen scaffold revealed that it enhances the activity of neural stem cells and promotes cell extension, without affecting cell differentiation. The collagen scaffold loaded with neural stem cells improved the hindlimb motor function in the rat model of T8 complete transection and promoted nerve regeneration. The collagen scaffold was completely degraded in vivo within 5 weeks of implantation, exhibiting good biodegradability. Rectal temperature, C-reactive protein expression and CD68 staining demonstrated that rats with spinal cord injury that underwent implantation of the collagen scaffold had no notable inflammatory reaction. These findings suggest that this novel collagen scaffold is a good carrier for neural stem cell transplantation, thereby enhancing spinal cord repair following injury. This study was approved by the Animal Ethics Committee of Nanjing Drum Tower Hospital (the Affiliated Hospital of Nanjing University Medical School), China (approval No. 2019AE02005) on June 15, 2019.
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http://dx.doi.org/10.4103/1673-5374.310698DOI Listing
November 2021

Synthesis and characterization of silver substituted strontium phosphate silicate apatite using solid-state reaction for osteoregenerative applications.

Bioengineered 2021 Dec;12(1):1111-1125

Department of Orthopaedics, China-Japan Friendship Hospital, Beijing, China.

Strontium phosphosilicate is one of the fastest-growing apatite in bone regeneration application due to the presence of strontium and silica components in the parent materials. However, bacterial infections cause setbacks to the bone regeneration process often leading to surgical revisions, and is a big issue that needs to be addressed. Silver on this front has proven to be a great substituent as seen in the case of calcium phosphate-based ceramics that addresses the bactericidal properties of a biomaterial. Apatite strontium phosphosilicate substituted with a stoichiometric amount of silver as a dopant was synthesized using a high-temperature solid-state reaction. The phase formation was characterized by XRD and FT-IR coupled with morphological features visualized using Electron Microscopy. Antibacterial properties were investigated quantitatively using Colony-forming unit method against both Gram-positive as well as Gram-negative bacteria. Cytotoxicity assay was performed against MG-63 Cell lines and it showed excellent biocompatibility at 25ug/ml with optimal doping of 2% silver. Further, apatite seeding and formation were characterized after immersion in simulated body fluid solution which showed apatite phase formation initiated after 4 days of treatment characterized by XRD and FT-IR studies. This apatite formation was also visualized and confirmed using SEM.
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http://dx.doi.org/10.1080/21655979.2021.1899670DOI Listing
December 2021

NF-κB activation in retinal microglia is involved in the inflammatory and neovascularization signaling in laser-induced choroidal neovascularization in mice.

Exp Cell Res 2021 Jun 31;403(1):112581. Epub 2021 Mar 31.

Department of Ophthalmology, School of Medicine, Sapporo Medical University, Sapporo, Japan.

Purpose: To evaluate Nuclear Factor NF-κB (NF-κB) signaling on microglia activation, migration, and angiogenesis in laser-induced choroidal neovascularization (CNV).

Methods: Nine-week-old C57BL/6 male mice were randomly assigned to IMD-0354 treated or untreated groups (5 mice, 10 eyes per group). CNV was induced with a 532-nm laser. Laser spots (power 250 mW, spot size 100 μm, time of exposure 50 ms) were created in each eye using a slit-lamp delivery system. Selective inhibitor of nuclear factor kappa-B kinase subunit beta (IKK2) inhibitor IMD-0354 (10 μg) was delivered subconjunctivally; vehicle-treated mice were the control. The treatment effect on CNV development was assessed at five days post-CNV induction in vivo in C57BL/6 and Cx3cr1 mice by fluorescent angiography, fundus imaging, and ex vivo by retinal flatmounts immunostaining and Western blot analysis of RPE/Choroidal/Scleral complexes (RCSC) lysates. In vitro evaluations of IMD-0354 effects were performed in the BV-2 microglial cell line using lipopolysaccharide (LPS) stimulation.

Results: IMD-0354 caused a significant reduction in the fluorescein leakage and size of the laser spot, as well as a reduction in microglial cell migration and suppression of phospho-IκBα, Vascular endothelial growth factor (VEGF-A), and Prostaglandin-endoperoxide synthase 2 (COX-2). In vivo and ex vivo observations demonstrated reduced lesion size in mice, CD68, and Allograft inflammatory factor 1 (IBA-1) positive microglia cells migration to the laser injury site in IMD-0354 treated eyes. The data further corroborate with GFP-positive cells infiltration of the CNV site in Cx3cr1 mice. In vitro IMD-0354 (10-25 ng/ml) treatment reduced NF-κB activation, expression of COX-2, caused decreased Actin-F presence and organization, resulting in reduced BV-2 cells migration capacity.

Conclusion: The present data indicate that NF-κB activation in microglia and it's migration capacity is involved in the development of laser CNV in mice. Its suppression by NF-κB inhibition might be a promising therapeutic strategy for wet AMD.
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http://dx.doi.org/10.1016/j.yexcr.2021.112581DOI Listing
June 2021

An Insight by Molecular Sensory Science Approaches to Contributions and Variations of the Key Odorants in Shiitake Mushrooms.

Foods 2021 Mar 15;10(3). Epub 2021 Mar 15.

School of Food Science and Technology, National Engineering Research Center of Seafood, Collaborative, Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian 116034, China.

An insight using molecular sensory science approaches to the contributions and variations of the key odorants in shiitake mushrooms is revealed in this study. Odorants were extracted by headspace solid phase microextraction (HS-SPME) and direct solvent extraction combined with solvent-assisted flavor evaporation (DSE-SAFE) in fresh and hot-air-dried shiitake mushrooms. Among them, 18 and 22 predominant odorants were determined by detection frequency analysis (DFA) and aroma extract dilution analysis (AEDA) combined with gas chromatography-olfactometry (GC-O) in the fresh and dried samples, respectively. The contributions of these predominant odorants in the food matrix were determined by quantification and odor activity values (OAVs) with aroma recombination verification. There were 13 and 14 odorants identified as key contributing odorants to overall aroma, respectively. 1-Octen-3-ol and 1-octen-3-one were the most key contributing odorants in the fresh samples in contributing mushroom-like odor. After hot-air-drying, the OAV and concentrations on dry basis of the key contributing odorants changed, due to oxidation, degradation, caramelization and Maillard reactions of fatty acids, polysaccharides and amino acids. 1-Octen-3-ol was reduced most significantly and degraded to 1-hydroxy-3-octanone, while phenylethyl alcohol increased the most and was formed by phenylalanine. In hot-air-dried samples, lenthionine became the most important contributor and samples were characterized by a sulfury odor. Overall contributions and variations of odorants to the aroma of shiitake mushrooms were revealed at the molecular level.
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http://dx.doi.org/10.3390/foods10030622DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7998831PMC
March 2021

Dual-channel illumination surface plasmon resonance holographic microscopy for resolution improvement.

Opt Lett 2021 Apr;46(7):1604-1607

Surface plasmon resonance holographic microscopy (SPRHM), combining digital holographic microscopy with surface plasmon resonance (SPR), can simultaneously obtain the amplitude and phase distributions of the reflected beam carrying specimen information in SPR. Due to the decaying length of the surface plasmon wave as large as tens of micrometers, the spatial resolution of SPRHM is lower than that of ordinary optical microscopes. In this work, we propose a scheme to improve the spatial resolution of SPRHM by applying dual-channel SPR excitations. Through the polarization multiplexing technique, two holograms carrying the information of SPR excited in orthogonal directions are simultaneously acquired. Via a numerical reconstruction and filtering algorithm for holograms, the lateral spatial resolution of SPRHM can be effectively enhanced to reach nearly 1 µm at a wavelength of 632.8 nm. This is comparable to the resolution of traditional optical microscopes, while possessing the advantages of wide-field imaging and high measurement sensitivity of SPR.
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http://dx.doi.org/10.1364/OL.419337DOI Listing
April 2021

[Professor 's experience in treatment of tic disorders in children with acupuncture from "meridian tendons"].

Zhongguo Zhen Jiu 2021 Feb;41(2):193-5

Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, Jiangsu Province, China.

Professor believes that the pathogenesis of tic disorders in children is "agitation of liver wind, lending to excessive wind and spasm of tendons" "deficiency of fluid, the tendons failing to be nourished" "spirit failing to govern, leading to tendons not be restrained" and advocates to treat this disease from the theory of "meridian tendons". He suggests the acupoints that related to "meridian tendons", and emphasizes the depth of needling and the procedure of acupuncture, and believes that the core of treating is to balance the and of "meridian tendons" and regulate spirit.
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http://dx.doi.org/10.13703/j.0255-2930.20200103-k0006DOI Listing
February 2021

UV-Assisted Li-Catalyzed Radical Grafting Polymerization of Vinyl Ethers: A New Strategy for Creating Hydrolysis-Resistant and Long-Lived Polymer Brushes as a "Smart" Surface Coating.

Langmuir 2021 04 31;37(14):4102-4111. Epub 2021 Mar 31.

Beijing Engineering Research Center of Syntheses and Applications of Waterborne Polymers College of Materials Science and Engineering, Beijing University of Chemical Technology, Beijing 100029, China.

A facile synthetic route was developed to prepare a surface-grafted brush layer of poly(vinyl ethers) (PVEs) directly by a radical mechanism, with the "naked" Li acting as a catalyst. Density functional theory calculations suggested that complexation of naked Li to VEs significantly reduced the highest unoccupied molecular orbital-lowest unoccupied molecular orbital (HOMO-LUMO) energy gap from 5.08 to 0.68 eV, providing a better prospect for electron transfer. The structure, morphology, and surface properties of grafted polymer layers were characterized using attenuated total reflection Fourier transform infrared spectroscopy, Raman spectroscopy, X-ray photoelectron spectroscopy, atomic force microscopy, and dynamic water contact angle (DCA). Moreover, ellipsometry data indicated that the thickness of the polymer brushes was in the range of 20-60 nm, which corresponds to the grafting densities of 0.65-1.15 chain/nm, and DCA decreased from 84.4 to 45.3°. Most importantly, no hydrolysis was observed for the modified surface after 30 days of exposure to phosphate-buffered saline solution, 0.1 mol/L NaOH(eq) and 0.1 mol/L HCl(eq), demonstrating excellent hydrolysis resistance with long service life. In addition, as a proof of concept, the side hydroxyl groups of grafted PVEs provide active sites for efficient fixation of bioactive molecules, e.g., glycosaminoglycan and serum protein.
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http://dx.doi.org/10.1021/acs.langmuir.0c03480DOI Listing
April 2021

Microfluidic synthesis of curcumin loaded polymer nanoparticles with tunable drug loading and pH-triggered release.

J Colloid Interface Sci 2021 Jul 15;594:474-484. Epub 2021 Mar 15.

Australian Institute for Bioengineering and Nanotechnology, University of Queensland, St. Lucia, Queensland 4072, Australia. Electronic address:

Polymer nanoparticles (NPs) have attracted significant interest in the past years for drug delivery and triggered release. However, it remains a significant challenge to produce polymer NPs with controlled properties and tunable drug loading. Traditional nanoprecipitation often leads to low drug loading. This study reports the development of a new microfluidic nanoprecipitation approach for making polymer NPs with tunable drug loading up to 50%. The synthesized curcumin-loaded shellac NPs remain very stable for the period of our experiments (10 days) under acidic conditions (pH 4.5), but release the payload at neutral pH in a sustained manner. This work provides a new strategy for making drug-loaded polymer NPs with tunable drug loading and triggered release.
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http://dx.doi.org/10.1016/j.jcis.2021.03.035DOI Listing
July 2021

Correction to: M2 Macrophagy-derived exosomal miRNA-5106 induces bone mesenchymal stem cells towards osteoblastic fate by targeting salt-inducible kinase 2 and 3.

J Nanobiotechnology 2021 Mar 27;19(1):88. Epub 2021 Mar 27.

Department of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.

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http://dx.doi.org/10.1186/s12951-021-00828-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8004442PMC
March 2021

ATAD2 interacts with C/EBPβ to promote esophageal squamous cell carcinoma metastasis via TGF-β1/Smad3 signaling.

J Exp Clin Cancer Res 2021 Mar 23;40(1):109. Epub 2021 Mar 23.

State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine,Sun Yat-sen University Cancer Center, Guangzhou, China.

Background: Distant metastasis is the leading cause of death for esophageal squamous cell carcinoma (ESCC) with limited treatment options and unsatisfactory effectiveness. Bromodomain (BRD) containing proteins are emerging targets for cancer therapy with promising effects. As a unique member of BRD family, the function and molecular mechanism of ATAD2 in cancer development is seldomly investigated.

Methods: The clinical impact of ATAD2 was assessed both at RNA and protein level in 75 and 112 ESCC patients separately. The biological function of ATAD2 was investigated in vitro and in vivo. Signaling pathway and downstream effectors of ATAD2 were identified by RNA sequencing, luciferase reporter, co-immunoprecipitation, chromatin immunoprecipitation, immunofluorescence and western blot assay.

Results: We found that elevated ATAD2 expression was significantly associated with lymph node metastasis, advanced clinical stage as well as poor survival of ESCC patients. Silencing ATAD2 significantly suppressed ESCC cell migration and invasion in vitro, and inhibited tumor growth and lung metastasis in vivo. Mechanically, we identified a new cofactor, C/EBPβ. ATAD2 directly interacted with C/EBPβ and promoted its nuclear translocation, which directly bound to the promoter region of TGF-β1 and activated its expression. Further, we demonstrated that TGF-β1 activated its downstream effectors in a Smad3 dependent manner. In addition, we further found that ATAD2 promoted ESCC metastasis through TGF-β signaling induced Snail expression and the subsequent epithelial-mesenchymal transition.

Conclusion: Our findings demonstrated the pro-metastatic function of ATAD2 and uncovered the new molecular mechanism by regulating C/EBPβ/TGF-β1/Smad3/Snail signaling pathway, thus providing a potential target for the treatment of ESCC metastasis.
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http://dx.doi.org/10.1186/s13046-021-01905-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7986551PMC
March 2021

Acceleration of western Arctic sea ice loss linked to the Pacific North American pattern.

Nat Commun 2021 03 9;12(1):1519. Epub 2021 Mar 9.

Department of Geology and Geophysics, University of Utah, Salt Lake City, USA.

Recent rapid Arctic sea-ice reduction has been well documented in observations, reconstructions and model simulations. However, the rate of sea ice loss is highly variable in both time and space. The western Arctic has seen the fastest sea-ice decline, with substantial interannual and decadal variability, but the underlying mechanism remains unclear. Here we demonstrate, through both observations and model simulations, that the Pacific North American (PNA) pattern is an important driver of western Arctic sea-ice variability, accounting for more than 25% of the interannual variance. Our results suggest that the recent persistent positive PNA pattern has led to increased heat and moisture fluxes from local processes and from advection of North Pacific airmasses into the western Arctic. These changes have increased lower-tropospheric temperature, humidity and downwelling longwave radiation in the western Arctic, accelerating sea-ice decline. Our results indicate that the PNA pattern is important for projections of Arctic climate changes, and that greenhouse warming and the resultant persistent positive PNA trend is likely to increase Arctic sea-ice loss.
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http://dx.doi.org/10.1038/s41467-021-21830-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7943814PMC
March 2021

Clinical Significance of Lymph Node Micrometastasis in pN0 Gastric Cancer Patients.

Gastroenterol Res Pract 2021 3;2021:6854646. Epub 2021 Mar 3.

Department of Gastrointestinal Surgery, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, China.

Purpose: To investigate the relationship between lymph node micrometastasis (LNMM) and clinicopathological factors and to evaluate the prognostic effects of LNMM in pN0 gastric cancer (GC) patients.

Methods: One hundred and seventy-two GC patients who received radical gastrectomy with D2 lymph node dissection were enrolled in the present study. 1371 negative lymph nodes from level 2 station confirmed by pathology were examined. The LNMM was diagnosed by telomeric repeat amplification protocol/enzyme-linked immunosorbent assay (TRAP-ELISA). The relationship between clinicopathological factors and LNMM was investigated by multivariate analysis. Survival analysis was performed to evaluate the effects of LNMM on prognosis.

Results: LNMM was detected in 423 lymph nodes from 72 patients. The results showed that invasion depth (OR = 3.755, = 0.004), TNM staging (OR = 3.152, = 0.002), lymphatic invasion (OR = 2.178, = 0.009), and tumor differentiation (OR = 1.266, = 0.013) were independent risk factors associated with LNMM. Survival analysis showed that patients with LNMM had significantly worse 5-year survival compared with those without LNMM (42% vs. 76.4%, < 0.05). Multivariate analysis demonstrated that LNMM, tumor size, Lauren type, invasion depth, and lymphatic invasion ( < 0.05) were independently factors associated with 5-year survival.

Conclusions: The findings showed that tumor invasion depth, TNM staging, lymphatic invasion, and tumor differentiation were independent risk factors associated with LNMM occurrence. Moreover, LNMM is a clinically negative prognostic factor in pN0 GC patients.
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http://dx.doi.org/10.1155/2021/6854646DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7946449PMC
March 2021