Publications by authors named "Dominique Bourgeois"

59Publications

Mechanistic Investigations of Green mEos4b Reveal a Dynamic Long-Lived Dark State.

J Am Chem Soc 2020 Jun 12;142(25):10978-10988. Epub 2020 Jun 12.

Institut de Biologie Structurale, Université Grenoble Alpes, CEA, CNRS, Grenoble 38044, France.

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http://dx.doi.org/10.1021/jacs.0c01880DOI Listing
June 2020

NMR Reveals Light-Induced Changes in the Dynamics of a Photoswitchable Fluorescent Protein.

Biophys J 2019 12 2;117(11):2087-2100. Epub 2019 Nov 2.

Institut de Biologie Structurale, Université Grenoble Alpes, CEA, CNRS, Grenoble, France. Electronic address:

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http://dx.doi.org/10.1016/j.bpj.2019.10.035DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6895687PMC
December 2019

A Long-Lived Triplet State Is the Entrance Gateway to Oxidative Photochemistry in Green Fluorescent Proteins.

J Am Chem Soc 2018 02 15;140(8):2897-2905. Epub 2018 Feb 15.

Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ. Paris Sud, Université Paris-Saclay , F-91198 Gif-sur-Yvette cedex, France.

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http://dx.doi.org/10.1021/jacs.7b12755DOI Listing
February 2018

Photoswitching of Green mEos2 by Intense 561 nm Light Perturbs Efficient Green-to-Red Photoconversion in Localization Microscopy.

J Phys Chem Lett 2017 Sep 5;8(18):4424-4430. Epub 2017 Sep 5.

Institut de Biologie Structurale, CNRS, Université Grenoble Alpes, CEA, IBS, 38044 Grenoble, France.

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http://dx.doi.org/10.1021/acs.jpclett.7b01701DOI Listing
September 2017

A General Mechanism of Photoconversion of Green-to-Red Fluorescent Proteins Based on Blue and Infrared Light Reduces Phototoxicity in Live-Cell Single-Molecule Imaging.

Angew Chem Int Ed Engl 2017 09 17;56(38):11634-11639. Epub 2017 Jul 17.

Department of Systems and Synthetic Microbiology, Max Planck Institute for Terrestrial Microbiology & LOEWE Center for Synthetic Microbiology (SYNMIKRO), Karl-von-Frisch-Str. 16, 35043, Marburg, Germany.

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http://dx.doi.org/10.1002/anie.201702870DOI Listing
September 2017

Deciphering Structural Photophysics of Fluorescent Proteins by Kinetic Crystallography.

Int J Mol Sci 2017 Jun 2;18(6). Epub 2017 Jun 2.

Institut de Biologie Structurale, Univ. Grenoble Alpes, CNRS, CEA, CNRS, IBS, F-38000 Grenoble, France.

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http://dx.doi.org/10.3390/ijms18061187DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5486010PMC
June 2017

Arginine 66 Controls Dark-State Formation in Green-to-Red Photoconvertible Fluorescent Proteins.

J Am Chem Soc 2016 Jan 6;138(2):558-65. Epub 2016 Jan 6.

Institut de Biologie Structurale, Université Grenoble Alpes , CEA, CNRS, 38044 Grenoble, France.

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http://dx.doi.org/10.1021/jacs.5b09923DOI Listing
January 2016

Double deletion of a chromosome 21 inserted in a chromosome 22 in an azoospermic patient.

Clin Case Rep 2015 Sep 20;3(9):757-61. Epub 2015 Aug 20.

Service de Génétique Médical, Centre Hospitalier Universitaire Purpan Toulouse, France.

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http://dx.doi.org/10.1002/ccr3.313DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4574793PMC
September 2015

Rational design of enhanced photoresistance in a photoswitchable fluorescent protein.

Methods Appl Fluoresc 2015 Jan 16;3(1):014004. Epub 2015 Jan 16.

Univ. Grenoble Alpes, IBS, F-38044 Grenoble, France. CNRS, IBS, F-38044 Grenoble, France. CEA, IBS, F-38044 Grenoble, France.

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http://dx.doi.org/10.1088/2050-6120/3/1/014004DOI Listing
January 2015

Phototransformable fluorescent proteins: Future challenges.

Curr Opin Chem Biol 2014 Jun 25;20:92-102. Epub 2014 Jun 25.

Univ. Grenoble Alpes, IBS, F-38044 Grenoble, France; CNRS, IBS, F-38044 Grenoble, France; CEA, IBS, F-38044 Grenoble, France. Electronic address:

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http://dx.doi.org/10.1016/j.cbpa.2014.05.016DOI Listing
June 2014

In cellulo evaluation of phototransformation quantum yields in fluorescent proteins used as markers for single-molecule localization microscopy.

PLoS One 2014 10;9(6):e98362. Epub 2014 Jun 10.

Université Grenoble Alpes, Institut de Biologie Structurale (IBS), Grenoble, France; CNRS, IBS, Grenoble, France; CEA, DSV, IBS, Grenoble, France.

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0098362PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4051587PMC
October 2015

Structural basis of photoswitching in fluorescent proteins.

Methods Mol Biol 2014 ;1148:177-202

Institut de Biologie Structurale, Université Grenoble Alpes, Grenoble, France.

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http://dx.doi.org/10.1007/978-1-4939-0470-9_12DOI Listing
December 2014

Structural evidence for a two-regime photobleaching mechanism in a reversibly switchable fluorescent protein.

J Am Chem Soc 2013 Oct 7;135(42):15841-50. Epub 2013 Oct 7.

Université Grenoble Alpes , Institut de Biologie Structurale (IBS), F-38027 Grenoble, France.

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http://dx.doi.org/10.1021/ja406860eDOI Listing
October 2013

Photoactivated structural dynamics of fluorescent proteins.

Biochem Soc Trans 2012 Jun;40(3):531-8

Pixel Team IBS, Institut de Biologie Structurale Jean-Pierre Ebel, Université Joseph Fourier, 41 rue Jules Horowitz, Grenoble, France.

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http://dx.doi.org/10.1042/BST20120002DOI Listing
June 2012

Reversible photoswitching in fluorescent proteins: a mechanistic view.

IUBMB Life 2012 Jun 25;64(6):482-91. Epub 2012 Apr 25.

Pixel Team, IBS, Institut de Biologie Structurale Jean-Pierre Ebel, CEA, CNRS, Université Joseph Fourier, Grenoble, France. dominique.bourgeois@ ibs.fr

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http://dx.doi.org/10.1002/iub.1023DOI Listing
June 2012

The nature of transient dark states in a photoactivatable fluorescent protein.

J Am Chem Soc 2011 Nov 31;133(46):18586-9. Epub 2011 Oct 31.

Institut de Biologie Structurale (IBS)-Jean-Pierre Ebel, CEA/CNRS/ Université Joseph Fourier, 41, rue Jules Horowitz, 38027 Grenoble Cedex 1, France.

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http://dx.doi.org/10.1021/ja2085355DOI Listing
November 2011

Low-temperature chromophore isomerization reveals the photoswitching mechanism of the fluorescent protein Padron.

J Am Chem Soc 2011 Oct 22;133(41):16362-5. Epub 2011 Sep 22.

Pixel Team, IBS, Institut de Biologie Structurale Jean-Pierre Ebel, CEA, CNRS, Université Joseph Fourier, 41 rue Jules Horowitz, F-38027 Grenoble, France.

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http://dx.doi.org/10.1021/ja207001yDOI Listing
October 2011

From EosFP to mIrisFP: structure-based development of advanced photoactivatable marker proteins of the GFP-family.

J Biophotonics 2011 Jun 14;4(6):377-90. Epub 2011 Feb 14.

National Oceanography Centre, University of Southampton, Southampton SO143ZH, UK.

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http://dx.doi.org/10.1002/jbio.201000122DOI Listing
June 2011

Raman-assisted crystallography suggests a mechanism of X-ray-induced disulfide radical formation and reparation.

Structure 2010 Nov;18(11):1410-9

Laboratoire de Cristallogenèse et Cristallographie des Protéines, IBS, Institut de Biologie Structurale Jean-Pierre Ebel, CEA, CNRS, Université Joseph Fourier, 41 Rue Jules Horowitz, F-38027 Grenoble, France.

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http://linkinghub.elsevier.com/retrieve/pii/S096921261000360
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http://dx.doi.org/10.1016/j.str.2010.09.010DOI Listing
November 2010

Diffusion pathways of oxygen species in the phototoxic fluorescent protein KillerRed.

Photochem Photobiol Sci 2010 Oct 31;9(10):1342-50. Epub 2010 Aug 31.

Institut de Biologie Structurale Jean-Pierre Ebel, CEA, CNRS, Université Joseph Fourier, 41 rue Jules Horowitz, F-38027, Grenoble, France.

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http://dx.doi.org/10.1039/c0pp00141dDOI Listing
October 2010

Raman-assisted crystallography of biomolecules at the synchrotron: instrumentation, methods and applications.

Biochim Biophys Acta 2011 Jun 5;1814(6):750-9. Epub 2010 Aug 5.

Biophysics Laboratories, Institute of Biomedical and Biomolecular Sciences, School of Biological Sciences, University of Portsmouth, PO1 2DY, UK.

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http://dx.doi.org/10.1016/j.bbapap.2010.07.021DOI Listing
June 2011

Data storage based on photochromic and photoconvertible fluorescent proteins.

J Biotechnol 2010 Sep 21;149(4):289-98. Epub 2010 Apr 21.

Laboratory of Photochemistry and Spectroscopy, Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200F, 3001 Heverlee, Belgium.

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http://dx.doi.org/10.1016/j.jbiotec.2010.04.001DOI Listing
September 2010

Low-temperature switching by photoinduced protonation in photochromic fluorescent proteins.

Photochem Photobiol Sci 2010 Feb 19;9(2):254-62. Epub 2010 Jan 19.

IBS, Institut de Biologie Structurale Jean-Pierre Ebel, CEA, CNRS, Université Joseph Fourier, 41 rue Jules Horowitz, F-38027, Grenoble, France.

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http://dx.doi.org/10.1039/b9pp00121bDOI Listing
February 2010

Structural basis of X-ray-induced transient photobleaching in a photoactivatable green fluorescent protein.

J Am Chem Soc 2009 Dec;131(50):18063-5

European Synchrotron Radiation Facility, 6 rue Jules Horowitz, BP 220, 38043 Grenoble Cedex, France.

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http://dx.doi.org/10.1021/ja907296vDOI Listing
December 2009

Watching CO enmeshed in hemoglobin.

Structure 2009 Nov;17(11):1427-8

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http://dx.doi.org/10.1016/j.str.2009.10.005DOI Listing
November 2009

Photoconversion of the fluorescent protein EosFP: a hybrid potential simulation study reveals intersystem crossings.

J Am Chem Soc 2009 Nov;131(46):16814-23

CNRS, UMR5075, Institut de Biologie Structurale Jean-Pierre Ebel, 41 rue Jules Horowitz, 38027 Grenoble Cedex 1, France.

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http://dx.doi.org/10.1021/ja905380yDOI Listing
November 2009

Intrinsic dynamics in ECFP and Cerulean control fluorescence quantum yield.

Biochemistry 2009 Oct;48(42):10038-46

Institut de Biologie Structurale Jean-Pierre Ebel, UMR 5075 CNRS-CEA-Universite Joseph Fourier, F-38027 Grenoble Cedex 1, France.

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http://dx.doi.org/10.1021/bi901093wDOI Listing
October 2009

Reverse pH-dependence of chromophore protonation explains the large Stokes shift of the red fluorescent protein mKeima.

J Am Chem Soc 2009 Aug;131(30):10356-7

Institut de Recherches en Technologies et Sciences pour le Vivant, Laboratoire de Physiologie Cellulaire Végétale, CEA, CNRS, INRA, Université Joseph Fourier, 17 rue des Martyrs, F-38054 Grenoble, France.

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http://pubs.acs.org/doi/abs/10.1021/ja903695n
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http://dx.doi.org/10.1021/ja903695nDOI Listing
August 2009

Structural basis for the phototoxicity of the fluorescent protein KillerRed.

FEBS Lett 2009 Sep 30;583(17):2839-42. Epub 2009 Jul 30.

Laboratoire de Cristallographie et Cristallogenèse des Protéines, IBS, Institut de Biologie Structurale Jean-Pierre Ebel, CEA, CNRS, Université Joseph Fourier, Grenoble, France.

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http://dx.doi.org/10.1016/j.febslet.2009.07.041DOI Listing
September 2009

Cryophotolysis of a caged oxygen compound for use in low temperature biological studies.

Photochem Photobiol Sci 2009 Aug 1;8(8):1150-6. Epub 2009 Jun 1.

Chemistry Research Laboratory, University of Oxford, 12 Mansfield Road, Oxford, UK OX1 3TA.

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http://dx.doi.org/10.1039/b821516bDOI Listing
August 2009

Raman-assisted X-ray crystallography for the analysis of biomolecules.

Methods Mol Biol 2009 ;544:253-67

Institut de Biologie Structurale Jean-Pierre Ebel, 41 Rue Jules Horowitz, 38027, Grenoble Cedex, France.

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http://dx.doi.org/10.1007/978-1-59745-483-4_16DOI Listing
November 2009

Structural basis of enhanced photoconversion yield in green fluorescent protein-like protein Dendra2.

Biochemistry 2009 Jun;48(22):4905-15

European Synchrotron Radiation Facility, 6 Rue Jules Horowitz, BP 220, 8043 Grenoble Cedex, France.

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http://dx.doi.org/10.1021/bi900383aDOI Listing
June 2009

Simultaneous measurements of solvent dynamics and functional kinetics in a light-activated enzyme.

Biophys J 2009 Mar;96(5):1902-10

Institut de Biologie Structurale Jean-Pierre Ebel, Centre d'Etudes Atomiques, Centre National de la Recherche Scientifique, Université Joseph Fourier, Grenoble, France.

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http://dx.doi.org/10.1016/j.bpj.2008.10.065DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2717306PMC
March 2009

Structural characterization of IrisFP, an optical highlighter undergoing multiple photo-induced transformations.

Proc Natl Acad Sci U S A 2008 Nov 18;105(47):18343-8. Epub 2008 Nov 18.

European Synchrotron Radiation Facility, 6 Rue Jules Horowitz, BP 220, 38043 Grenoble Cedex, France.

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http://dx.doi.org/10.1073/pnas.0805949105DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2587625PMC
November 2008

Shoot-and-Trap: use of specific x-ray damage to study structural protein dynamics by temperature-controlled cryo-crystallography.

Proc Natl Acad Sci U S A 2008 Aug 13;105(33):11742-7. Epub 2008 Aug 13.

Laboratoire de Biophysique Moléculaire and Laboratoire de Cristallogenèse et de Cristallographie des Protéines, Centre National de la Recherche Scientifique, Université Joseph Fourier, F-38027 Grenoble, France.

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http://dx.doi.org/10.1073/pnas.0804828105DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2575335PMC
August 2008

Use of a 'caged' analogue to study the traffic of choline within acetylcholinesterase by kinetic crystallography.

Acta Crystallogr D Biol Crystallogr 2007 Nov 17;63(Pt 11):1115-28. Epub 2007 Oct 17.

Laboratoire de Biophysique Moléculaire, Institut de Biologie Structurale Jean-Pierre EBEL, UMR 5075 CNRS-CEA-UJF, 38027 Grenoble, France.

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http://dx.doi.org/10.1107/S0907444907044472DOI Listing
November 2007

Time-resolved methods in biophysics. 6. Time-resolved Laue crystallography as a tool to investigate photo-activated protein dynamics.

Photochem Photobiol Sci 2007 Oct 25;6(10):1047-56. Epub 2007 Jul 25.

IBS, Institut de Biologie Structurale Jean-Pierre Ebel, CEA, CNRS, Université Joseph Fourier, 41 rue Jules Horowitz, F-38027, Grenoble, France.

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http://xlink.rsc.org/?DOI=b704249c
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http://dx.doi.org/10.1039/b704249cDOI Listing
October 2007

Raman-assisted crystallography reveals end-on peroxide intermediates in a nonheme iron enzyme.

Science 2007 Apr;316(5823):449-53

Institut de Biologie Structurale (IBS) Jean-Pierre Ebel, Commissariat à l'Energie Atomique (CEA), Centre National de la Recherche Scientifique (CNRS), Université Joseph Fourier, 41 rue Jules Horowitz, F-38027 Grenoble, France.

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http://dx.doi.org/10.1126/science.1138885DOI Listing
April 2007

X-ray radiation-induced damage in DNA monitored by online Raman.

J Synchrotron Radiat 2007 Jan 15;14(Pt 1):99-108. Epub 2006 Dec 15.

EMBL, 6 rue Jules Horowitz, BP 181, 38042 Grenoble CEDEX 9, France.

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http://dx.doi.org/10.1107/S0909049506043251DOI Listing
January 2007

Extended subnanosecond structural dynamics of myoglobin revealed by Laue crystallography.

Proc Natl Acad Sci U S A 2006 Mar 17;103(13):4924-9. Epub 2006 Mar 17.

Institut de Biologie Structurale, Unité Mixte de Recherche 5075, Centre National de la Recherche Scientifique/Commissariat à l'Energie Atomique/Université Joseph Fourier, 41 Rue Jules Horowitz, 38027 Grenoble Cedex 1, France.

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http://dx.doi.org/10.1073/pnas.0508880103DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1458771PMC
March 2006

UV laser-excited fluorescence as a tool for the visualization of protein crystals mounted in loops.

Acta Crystallogr D Biol Crystallogr 2006 Mar 22;62(Pt 3):253-61. Epub 2006 Feb 22.

LCCP, UMR 5075, IBS-CEA/CNRS/UJF, Grenoble, France.

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http://dx.doi.org/10.1107/S0907444905041429DOI Listing
March 2006

The 1.6A X-ray structure of the unusual c-type cytochrome, cytochrome cL, from the methylotrophic bacterium Methylobacterium extorquens.

J Mol Biol 2006 Mar 5;357(1):151-62. Epub 2006 Jan 5.

School of Biological Sciences, University of Southampton, Bassett Crescent East, Southampton SO16 7PX, UK.

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http://dx.doi.org/10.1016/j.jmb.2005.12.055DOI Listing
March 2006

Advances in kinetic protein crystallography.

Curr Opin Struct Biol 2005 Oct;15(5):538-47

LCCP, UMR 5075, IBS, 41 avenue Jules Horowitz, 38027 Grenoble Cedex 1, France.

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http://dx.doi.org/10.1016/j.sbi.2005.08.002DOI Listing
October 2005

The crystal structure of Mycobacterium tuberculosis thymidylate kinase in complex with 3'-azidodeoxythymidine monophosphate suggests a mechanism for competitive inhibition.

Biochemistry 2005 Jan;44(1):130-7

LCCP, UMR 5075, IBS-CEA/CNRS/UJF, 41 avenue Jules Horowitz, 38027 Grenoble Cedex 1, France.

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http://dx.doi.org/10.1021/bi0484163DOI Listing
January 2005

Structure of superoxide reductase bound to ferrocyanide and active site expansion upon X-ray-induced photo-reduction.

Structure 2004 Sep;12(9):1729-40

LCCP, UMR 5075, IBS-CEA/CNRS/Université J. Fourier, 41 Avenue Jules Horowitz, 38027 Grenoble, Cedex 1, France.

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http://dx.doi.org/10.1016/j.str.2004.07.013DOI Listing
September 2004

Structural basis for the mechanism of Ca(2+) activation of the di-heme cytochrome c peroxidase from Pseudomonas nautica 617.

Structure 2004 Jun;12(6):961-73

REQUIMTE/CQFB, Departamento de Química, FCT, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.

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http://dx.doi.org/10.1016/j.str.2004.03.025DOI Listing
June 2004

Temperature derivative fluorescence spectroscopy as a tool to study dynamical changes in protein crystals.

Biophys J 2004 May;86(5):3176-85

Laboratoire de Biophysique Moléculaire and Laboratoire de Cristallographie et Cristallogenèse des Protéines, UMR 5075, Institut de Biologie Structurale, 38027 Grenoble, France.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1304182PMC
http://dx.doi.org/10.1016/S0006-3495(04)74365-9DOI Listing
May 2004

Characterization of photocycle intermediates in crystalline photoactive yellow protein.

Photochem Photobiol 2003 Aug;78(2):131-7

Laboratory for Microbiology, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands.

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http://dx.doi.org/10.1562/0031-8655(2003)078<0131:copiic>2.0.co;2DOI Listing
August 2003

Complex landscape of protein structural dynamics unveiled by nanosecond Laue crystallography.

Proc Natl Acad Sci U S A 2003 Jul 7;100(15):8704-9. Epub 2003 Jul 7.

Laboratoire de Cristallographie et de Cristallogénèse des Protéines, UMR 9015, Institut de Biologie Structurale/Commissariat à l'Energie Atomique/Université Joseph Fourier, and European Synchrotron Radiation Facility, Grenoble, France.

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http://dx.doi.org/10.1073/pnas.1430900100DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC166376PMC
July 2003

Cryophotolysis of caged compounds: a technique for trapping intermediate states in protein crystals.

Acta Crystallogr D Biol Crystallogr 2002 Apr 22;58(Pt 4):607-14. Epub 2002 Mar 22.

LCCP, UMR 9015, Institut de Biologie Structurale, 41 Avenue Jules Horowitz, 38027 Grenoble CEDEX 1, France.

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http://dx.doi.org/10.1107/s0907444902002135DOI Listing
April 2002