Publications by authors named "Dolores Lozano"

4 Publications

  • Page 1 of 1

Molecular diagnosis in non-small-cell lung cancer: expert opinion on and testing.

J Clin Pathol 2021 Apr 19. Epub 2021 Apr 19.

Department of Pathology, Hospital Universitario Virgen del Rocío, Sevilla, Spain.

The effectiveness of targeted therapies with tyrosine kinase inhibitors in non-small-cell lung cancer (NSCLC) depends on the accurate determination of the genomic status of the tumour. For this reason, molecular analyses to detect genetic rearrangements in some genes (ie, , , ) have become standard in patients with advanced disease. Since immunohistochemistry is easier to implement and interpret, it is normally used as the screening procedure, while fluorescence in situ hybridisation (FISH) is used to confirm the rearrangement and decide on ambiguous immunostainings. Although FISH is considered the most sensitive method for the detection of and rearrangements, the interpretation of results requires detailed guidelines. In this review, we discuss the various technologies available to evaluate and genomic rearrangements using these techniques. Other techniques such as real-time PCR and next-generation sequencing have been developed recently to evaluate and gene rearrangements, but some limitations prevent their full implementation in the clinical setting. Similarly, liquid biopsies have the potential to change the treatment of patients with advanced lung cancer, but further research is required before this technology can be applied in routine clinical practice. We discuss the technical requirements of laboratories in the light of quality assurance programmes. Finally, we review the recent updates made to the guidelines for the determination of molecular biomarkers in patients with NSCLC.
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http://dx.doi.org/10.1136/jclinpath-2021-207490DOI Listing
April 2021

Biological Marker Analysis as Part of the CIBERES-RTIC Cancer-SEPAR Strategic Project on Lung Cancer.

Arch Bronconeumol 2015 Sep 19;51(9):462-7. Epub 2015 Jan 19.

CIBER de Enfermedades Respiratorias-CIBERES, Instituto de Salud Carlos III, Madrid, España.

The aim of the Clinical and Molecular Staging of Stage I-IIp Lung Cancer Project is to identify molecular variables that improve the prognostic and predictive accuracy of TMN classification in stage I/IIp non-small cell lung cancer (NSCLC). Clinical data and lung tissue, tumor and blood samples will be collected from 3 patient cohorts created for this purpose. The prognostic protein signature will be validated from these samples, and micro-RNA, ALK, Ros1, Pdl-1, and TKT, TKTL1 y G6PD expression will be analyzed. Tissue inflammatory markers and stromal cell markers will also be analyzed. Methylation of p16, DAPK, RASSF1a, APC and CDH13 genes in the tissue samples will be determined, and inflammatory markers in peripheral blood will also be analyzed. Variables that improve the prognostic and predictive accuracy of TNM in NSCLC by molecular staging may be identified from this extensive analytical panel.
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http://dx.doi.org/10.1016/j.arbres.2014.11.010DOI Listing
September 2015

Protein cross-linking, peroxidase and beta-1,3-endoglucanase involved in resistance of pea against Orobanche crenata.

J Exp Bot 2006 23;57(6):1461-9. Epub 2006 Mar 23.

CSIC, Instituto de Agricultura Sostenible, E-14080 Córdoba, Apdo. 4084, Spain.

Root holoparasitic angiosperms, like Orobanche spp, completely lack chlorophyll and totally depend on their host for their supply of nutrients. O. crenata is a severe constraint to the cultivation of legumes and breeding for resistance remains the most economical, feasible, and environmentally friendly method of control. Due to the lack of resistance in commercial pea cultivars, the use of wild relatives for breeding is necessary, and an understanding of the mechanisms underlying host resistance is needed in order to improve screening for resistance in breeding programmes. Compatible and incompatible interactions between O. crenata and pea have been studied using cytochemical procedures. The parasite was stopped in the host cortex before reaching the central cylinder, and accumulation of H2O2, peroxidases, and callose were detected in neighbouring cells. Protein cross-linking in the host cell walls appears as the mechanism of defence, halting penetration of the parasite. In situ hybridization studies have also shown that a peroxidase and a beta-glucanase are differently expressed in cells of the resistant host (Pf651) near the penetration point. The role of these proteins in the resistance to O. crenata is discussed.
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http://dx.doi.org/10.1093/jxb/erj127DOI Listing
August 2006

Mucilage production during the incompatible interaction between Orobanche crenata and Vicia sativa.

J Exp Bot 2006 10;57(4):931-42. Epub 2006 Feb 10.

CSIC, Instituto de Agricultura Sostenible, Apdo. 4084, E-14080 Córdoba, Spain.

Orobanche spp. (broomrapes) are holoparasites lacking in chlorophyll and totally dependent on their host for their supply of nutrients. O. crenata is a severe constraint to legumes cultivation and breeding for resistance remains as one of the best available methods of control. However, little is known about the basis of host resistance to broomrapes. It is a multicomponent event, and resistance based on hampering development and necrosis of broomrape tubercles has been reported. In the present work, the formation of mucilage and occlusion of host xylem vessels associated with the death of O. crenata tubercles were studied histologically. Samples of necrotic O. crenata tubercles established on resistant and susceptible vetch genotypes were collected. The samples were fixed, sectioned and stained using different procedures. The sections were observed at the light microscopy level, either under bright field, epi-fluorescence or confocal laser scanning microscopy. A higher proportion of necrotic tubercles was found on the resistant genotype and this was associated with a higher percentage of occluded vessels. Mucilage is composed mainly by carbohydrates (non-esterified pectins) and the presence of polyphenols was also detected. The mucilage and other substances composed by parasite secretions and host-degraded products was found to block host vessels and obstruct the parasite supply channel, being a quantitative defensive response against O. crenata in vetch, and probably also in other legumes and plants. The presence of foreign substances (i.e. parasite secretions) and host-degraded products (i.e. carbohydrates from cell walls) inside host vessels seems to activate this response and leads to xylem occlusion and further death of established Orobanche tubercles.
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http://dx.doi.org/10.1093/jxb/erj078DOI Listing
June 2006