Publications by authors named "Ding Lei"

627 Publications

Quality inspection of micro solder joints in laser spot welding by laser ultrasonic method.

Ultrasonics 2021 Aug 30;118:106567. Epub 2021 Aug 30.

Department of Materials Science and Engineering, and National Laboratory of Solid State Microstructures, Nanjing University, Nanjing 210093, China.

The quality inspection of micro solder joints in laser spot welding (LSW) is a problem of great concern in industrial application. In this paper, a laser ultrasonic technology (LUT) was proposed to inspect the quality of micro solder joints in LSW. Firstly, based on the thermoelastic model of acoustic wave propagation in solid, a theoretical model was built and used to analyze the propagation properties of the Lamb wave in the whole field by finite element method (FEM), the transmitting properties of the excited Lamb wave via solder joint were affected by the effective contacted area of solder joint. Secondly, LUT was used to inspect the 1.2 mm/0.4 mm welding spot of standard/false 304 stainless steel welded components. By comparing the propagating properties of excited ultrasonic wave in different samples with different weld quality, the standard and false welding can be visually distinguished. Finally, a industrial CT was used to check the quality of the samples used in our experiment. Inclusions and pores have been found in the false solder joints, which will reduce the effective contacted area of solder joint, and then affected the propagation of ultrasonic wave. By combining the CT results and the experimental analysis, the experimental results detected by LUT are in good agreement with the simulation results. So, the LUT is a potential method in field of the quality inspection of micro solder joints in LSW.
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http://dx.doi.org/10.1016/j.ultras.2021.106567DOI Listing
August 2021

A polarized anchor for hematopoietic stem cells: Synapse between stem cells and their niche?

Authors:
Yeojin Lee Lei Ding

J Cell Biol 2021 Oct 13;220(10). Epub 2021 Sep 13.

Columbia Stem Cell Initiative, Department of Rehabilitation and Regenerative Medicine, Department of Microbiology and Immunology, Columbia University Irving Medical Center, New York, NY.

Multipotent hematopoietic stem cells are maintained by the bone marrow niche, but how niche-derived membrane-bound stem cell factor (mSCF) regulates HSCs remains unclear. In this issue, Hao et al. (2021. J. Cell Biol.https://doi.org/10.1083/jcb.202010118) describe that mSCF, synergistically with VCAM-1, induces large, polarized protrusions that serve as anchors for HSCs to their niche.
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http://dx.doi.org/10.1083/jcb.202108031DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8441858PMC
October 2021

Morphological and Molecular Phylogenetic Data of the Chinese Medicinal Fungus Reveal Its New Systematic Position in the Family Ophiocordycipitaceae.

Mycobiology 2021 11;49(4):297-307. Epub 2021 Jun 11.

Yunnan Herbal Laboratory, School of Ecology and Environmental Science, Yunnan University, Kunming, China.

A cordycipitoid fungus infecting Hepialidae sp. in Nepal was supposed to be identical to , originally described from southwestern China, and thus, transferred to the genus or in previous studies. However, our multi-gene (nrSSU-nrLSU---) phylogenetic and morphological studies based on the type specimen and additional collections of revealed that the fungus belongs to the genus (Ophiocordycipitaceae). Therefore, a new combination was made, and a detailed description of this species was provided.
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http://dx.doi.org/10.1080/12298093.2021.1923388DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8409936PMC
June 2021

Field-tunable toroidal moment in a chiral-lattice magnet.

Nat Commun 2021 Sep 9;12(1):5339. Epub 2021 Sep 9.

Oak Ridge National Laboratory, Neutron Scattering Division, Oak Ridge, TN, USA.

Ferrotoroidal order, which represents a spontaneous arrangement of toroidal moments, has recently been found in a few linear magnetoelectric materials. However, tuning toroidal moments in these materials is challenging. Here, we report switching between ferritoroidal and ferrotoroidal phases by a small magnetic field, in a chiral triangular-lattice magnet BaCoSiO with tri-spin vortices. Upon applying a magnetic field, we observe multi-stair metamagnetic transitions, characterized by equidistant steps in the net magnetic and toroidal moments. This highly unusual ferri-ferroic order appears to come as a result of an unusual hierarchy of frustrated isotropic exchange couplings revealed by first principle calculations, and the antisymmetric exchange interactions driven by the structural chirality. In contrast to the previously known toroidal materials identified via a linear magnetoelectric effect, BaCoSiO is a qualitatively new multiferroic with an unusual coupling between several different orders, and opens up new avenues for realizing easily tunable toroidal orders.
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http://dx.doi.org/10.1038/s41467-021-25657-6DOI Listing
September 2021

Spatial patterns and driving factors of aboveground and belowground biomass over the eastern Eurasian steppe.

Sci Total Environ 2021 Aug 24;803:149700. Epub 2021 Aug 24.

Natural Resources Comprehensive Survey Command Center, China Geological Survey, Beijing 100037, PR China. Electronic address:

The Eurasian steppe is the largest steppe region in the world and is an important part of the global grassland ecosystem. The eastern Eurasian steppe has favorable hydrothermal conditions and has the highest productivity and the richest biodiversity. Located in the arid and semi-arid region, the eastern Eurasian steppe has experienced large-scale grassland degradation due to dramatic climate change and intensive human activities during the past 20 years. Hence, accurate estimation of aboveground biomass (AGB, gC m) and belowground biomass (BGB, gC m) is necessary. In this study, plenty of AGB and BGB in-situ measurements were collected among dominated grassland types during summer in 2013 and 2016-2018 in the eastern Eurasian steppe. Vegetation indices from the Moderate Resolution Imaging Spectroradiometer (MODIS), Digital Elevation Model (DEM) and climate variables were chosen as independent variables to establish predictive models for AGB and BGB with random forest (RF). Both AGB (R = 0.47, MAE = 21.06 gC m, and RMSE = 27.52 gC m) and BGB (R = 0.44, MAE = 173.02 gC m, and RMSE = 244.20 gC m) models showed acceptable accuracy. Then the RF models were applied to generate spatially explicit AGB and BGB estimates for the study area over the last two decades (2000-2018). Both AGB and BGB showed higher values in the Greater Khingan Mountains and decreased gradually to the east and west sides. The mean values for AGB and BGB were 62.16 gC m and 531.35 gC m, respectively. The climatic factors were much more important in controlling biomass than anthropogenic drivers, and shortage of water and raising temperature were the main limiting factor of AGB and BGB, respectively, in the peak growth season. These findings provide scientific data for the scientific management of animal husbandry and can contribute to the sustainable development of grassland ecology in the eastern Eurasian steppe.
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http://dx.doi.org/10.1016/j.scitotenv.2021.149700DOI Listing
August 2021

The progress of research on histone methylation in ischemic stroke pathogenesis.

J Physiol Biochem 2021 Sep 2. Epub 2021 Sep 2.

Department of Pathophysiology, School of Basic Medical Sciences, Xuzhou Medical University, Tongshan Road No. 209, Xuzhou, 221004, China.

Stroke, also known as cerebral stroke or cerebrovascular accident, refers to acute ischemic or hemorrhagic encephalopathy caused by a disturbance to cerebral blood flow. Ischemic stroke is the most common type of cerebral stroke, accounting for approximately 80% of the total incidence of clinical stroke. High morbidity, disability, and mortality rates place heavy burdens on the families of patients and society. An increasing number of studies have shown that histone modification plays an important role in the pathogenesis of ischemic stroke, but most studies on histone modification focus on acetylation, and studies on the role of histone methylation in the pathogenesis of ischemic stroke are limited. Here, we review the role of histone methylation and related histone methyltransferase (HMT) inhibitors in the pathogenesis of ischemic stroke and related HMT inhibitors in the treatment of ischemic stroke, which may open up a new avenue to the study of ischemic stroke.
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http://dx.doi.org/10.1007/s13105-021-00841-wDOI Listing
September 2021

Overlooked long-chain chlorinated paraffin (LCCP) contamination in foodstuff from China.

Sci Total Environ 2021 Aug 19;801:149775. Epub 2021 Aug 19.

Jinan Ecological Environmental Protection Grid Supervision Center, Lvyou Road 17199, Jinan 250098, China.

Data on long-chain chlorinated paraffins (LCCPs) is extremely sparse, despite their use and emission are increasing with the phasing out of short-chain chlorinated paraffins (SCCPs). In this study, we analyzed chlorinated paraffins (CPs) in foodstuff samples (551 pooled samples, 93 items) divided into eight categories collected from Jinan, Shandong Province of China, by atmospheric-pressure chemical ionization quadrupole time-of-flight mass spectrometry (APCI-qToF-MS), to investigate the occurrence, contamination patterns and homologue patterns of LCCPs in foodstuff commonly consumed in traditional Chinese diet. LCCP intake through diet was estimated as well. LCCPs were detected in all pooled samples with geometric mean (GM) concentrations ranging from 1.8 to 21.9 ng/g wet weight (ng/g ww), contributing to 9-28% of the total CP mass in the studied foodstuff categories. The contamination patterns of LCCPs differed from SCCPs and medium-chain chlorinated paraffins (MCCPs), as reflected by the patterns of mass distribution, and by the lack of correlations between LCCP and S/MCCP concentrations in various foodstuff categories. The homologue profiles of LCCPs were extremely complex and diverse, with frequent detection of CCl very-long-chain chlorinated paraffin (vLCCP) congeners. The homologue profiles of eggs stood out for their high abundance of CCl LCCP congeners. LCCPs contributed 6.0-25.2% (8.9% for median estimation) to the estimated dietary intake (EDI) for total CPs through diet based on estimations using different percentiles of CP concentrations. The median estimate of dietary LCCP intake for adults in Jinan was 287.9 ng/kg_bw/day, reaching ~10- to 100-fold of that in Sweden and Canada. Considering the continuing production, use and emission of LCCPs, as well as the similar toxicity effects induced by LCCPs as SCCPs and MCCPs, attention should be paid to the health risk posed by LCCPs, or all CPs as a class of contaminants.
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http://dx.doi.org/10.1016/j.scitotenv.2021.149775DOI Listing
August 2021

Thrombopoietin from hepatocytes promotes hematopoietic stem cell regeneration after myeloablation.

Elife 2021 Aug 31;10. Epub 2021 Aug 31.

Columbia University, New York, United States.

The bone marrow niche plays a critical role in hematopoietic recovery and hematopoietic stem cell (HSC) regeneration after myeloablative stress. However, it is not clear whether systemic factors beyond the local niche are required for these essential processes . Thrombopoietin (THPO) is a critical cytokine promoting hematopoietic rebound after myeloablation and its transcripts are expressed by multiple cellular sources. The upregulation of bone marrow-derived THPO has been proposed to be crucial for hematopoietic recovery and HSC regeneration after stress. Nonetheless, the cellular source of THPO in myeloablative stress has never been investigated genetically. We assessed the functional sources of THPO following two common myeloablative perturbations: 5-fluorouracil (5-FU) administration and irradiation. Using a translational reporter, we found that the liver but not the bone marrow is the major source of THPO protein after myeloablation. Mice with conditional deletion from osteoblasts and/or bone marrow stromal cells showed normal recovery of HSCs and hematopoiesis after myeloablation. In contrast, mice with conditional deletion from hepatocytes showed significant defects in HSC regeneration and hematopoietic rebound after myeloablation. Thus, systemic THPO from the liver is necessary for HSC regeneration and hematopoietic recovery in myeloablative stress conditions.
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http://dx.doi.org/10.7554/eLife.69894DOI Listing
August 2021

Mechanism of noncoding RNA-associated N-methyladenosine recognition by an RNA processing complex during IgH DNA recombination.

Mol Cell 2021 Aug 20. Epub 2021 Aug 20.

Department of Microbiology and Immunology, Vagelos College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA. Electronic address:

Immunoglobulin heavy chain (IgH) locus-associated G-rich long noncoding RNA (SμGLT) is important for physiological and pathological B cell DNA recombination. We demonstrate that the METTL3 enzyme-catalyzed N-methyladenosine (mA) RNA modification drives recognition and 3' end processing of SμGLT by the RNA exosome, promoting class switch recombination (CSR) and suppressing chromosomal translocations. The recognition is driven by interaction of the MPP6 adaptor protein with nuclear mA reader YTHDC1. MPP6 and YTHDC1 promote CSR by recruiting AID and the RNA exosome to actively transcribe SμGLT. Direct suppression of mA modification of SμGLT or of mA reader YTHDC1 reduces CSR. Moreover, METTL3, an essential gene for B cell development in the bone marrow and germinal center, suppresses IgH-associated aberrant DNA breaks and prevents genomic instability. Taken together, we propose coordinated and central roles for MPP6, mA modification, and mA reader proteins in controlling long noncoding RNA processing, DNA recombination, and development in B cells.
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http://dx.doi.org/10.1016/j.molcel.2021.07.037DOI Listing
August 2021

Magnetic solid-phase extraction based on sulfur-functionalized magnetic metal-organic frameworks for the determination of methylmercury and inorganic mercury in water and fish samples.

J Chromatogr A 2021 Sep 12;1654:462465. Epub 2021 Aug 12.

School of Precision Instrument and Opto-Electronics Engineering, Tianjin University, Tianjin 300072, PR China. Electronic address:

A novel magnetic metal-organic frameworks ([email protected]) was successfully prepared by coating FeO nanospheres with sulfur-functionalized UiO-66. The [email protected] possesses both the magnetic properties of FeO and the diverse properties of metal-organic framework (MOF) in one material, which has the superiority of high surface area, easy-operation and strong adsorb ability with mercury, is used for the magnetic solid-phase extraction of methylmercury (MeHg) and inorganic mercury (Hg) in water and fish samples. The analyzes were conducted by high performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP-MS). The different pretreatment conditions influencing the extraction recoveries of Hg and MeHg, including adsorbent amount, pH, extraction time, elution solvent, elution volume, desorption time, co-existing ions and dissolved organic materials were investigated. Under the optimized conditions, the limits of detection (LODs) of Hg and MeHg for water samples were 1.4 and 2.6 ng L, and the limits of quantification (LOQs) of Hg and MeHg for water samples were 4.7 and 8.7 ng L. The enrichment factors (EFs) were 45.7 and 47.6 fold for Hg and MeHg, respectively. The accuracy of the proposed method was demonstrated by analyzing the certified reference material of fish tissue (GBW10029) and by determining the analyte content in spiked water and fish samples. The determined values were in good agreement with the certified values and the recoveries for the spiked samples were in the range of 84.5-96.8%.
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http://dx.doi.org/10.1016/j.chroma.2021.462465DOI Listing
September 2021

MiR-34a-5p and miR-452-5p: The Novel Regulators of Pancreatic Endocrine Dysfunction in Diabetic Zucker Rats?

Int J Med Sci 2021 11;18(14):3171-3181. Epub 2021 Jul 11.

Dongfang Hospital of Beijing University of Chinese Medicine, Beijing, Beijing 100078, China.

The pancreatic endocrinal system dominates the regulation of blood glucose levels in vivo, and the dysfunction of pancreatic endocrine β-cells is a major cause of the occurrence and development of Type 2 diabetes (T2D). Although microRNA (miRNA) have been found to be key regulators of pancreatic β-cells proliferation, differentiation and apoptosis, the underlying mechanism remains enigmatic. The aim of this study was to identify several novel miRNAs which might be involved in the etiopathogenesis of diabetic β-cells dysfunction. The miRNA expression profiles in the pancreas of high-fat diet (HFD) fed Zucker diabetic fatty (ZDF) rats and Zucker lean (ZL) rats feed with normal-fat diet (NFD) were detected by using miRNA microarray chip, and individually verified the most significant factors by quantitative real-time polymerase chain reaction (qRT-PCR) assay. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were used to predict the target genes related to each of the identified miRNAs and the functions of these target genes in different metabolic signaling pathways. Compared with the ZL rats, a total of 24 differentially expressed miRNAs were detected in ZDF rats. Among which miR-34a-5p and miR-452-5p were the most significantly up-regulated and down-regulated respectively. These miRNAs have not been reported in rats' pancreas before. By GO and KEGG enrichment analyses, we found that miR-34a-5p could negatively regulate pancreatic β-cell proliferation through the involvement of Wnt signaling pathway. In addition, it was also found to regulate insulin secretion through the insulin signaling pathway to modulate blood glucose levels. At the same time, miR-452-5p was found to positively regulate the activity of the key rate-limiting enzyme branched-chain α-keto acid dehydrogenase-β (BCKDHB) in the catabolism of branched chain amino acids (BCAA), leading to mitochondrial dysfunction in pancreatic β-cells. miR-34a-5p and miR-452-5p were identified as the novel regulators of pancreatic endocrine dysfunction. These miRNAs might have the potential to be utilized as the new predictive biomarkers for the diagnosis of the occurrence and development of T2D, as well as the therapeutic targets for T2D treatment.
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http://dx.doi.org/10.7150/ijms.62843DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8364455PMC
July 2021

The Application of High-Throughput Technologies for the Study of Microbiome and Cancer.

Front Genet 2021 28;12:699793. Epub 2021 Jul 28.

State Key Laboratory of Oncogenes and Related Genes, Centre for Single-Cell Omics, School of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Human gut microbiome research, especially gut microbiome, has been developing at a considerable pace over the last decades, driven by a rapid technological advancement. The emergence of high-throughput technologies, such as genomics, transcriptomics, and others, has afforded the generation of large volumes of data, and in relation to specific pathologies such as different cancer types. The current review identifies high-throughput technologies as they have been implemented in the study of microbiome and cancer. Four main thematic areas have emerged: the characterization of microbial diversity and composition, microbial functional analyses, biomarker prediction, and, lastly, potential therapeutic applications. The majority of studies identified focus on the microbiome diversity characterization, which is reaching technological maturity, while the remaining three thematic areas could be described as emerging.
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http://dx.doi.org/10.3389/fgene.2021.699793DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8355622PMC
July 2021

PD-1/PD-L1 inhibitors-based treatment for advanced renal cell carcinoma: Mechanisms affecting efficacy and combination therapies.

Cancer Med 2021 Sep 12;10(18):6384-6401. Epub 2021 Aug 12.

Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

With the widespread use of PD-1/PD-L1 monoclonal antibodies (mAbs) in the treatment of multiple malignant tumors, they were also gradually applied to advanced renal cell carcinoma (aRCC). Nowadays, multiple PD-1/PD-L1 mAbs, such as nivolumab, avelumab, and pembrolizumab, have achieved considerable efficacy in clinical trials. However, due to the primary, adaptive, and acquired resistance to these mAbs, the efficacy of this immunotherapy is not satisfactory. Theories also vary as to why the difference in efficacy occurs. The alterations of PD-L1 expression and the interference of cellular immunity may affect the efficacy. These mechanisms demand to be revealed to achieve a sustained and complete objective response in patients with aRCC. Tyrosine kinase inhibitors have been proven to have synergistic mechanisms with PD-1/PD-L1 mAb in the treatment of aRCC, and CTLA-4 mAb has been shown to have a non-redundant effect with PD-1/PD-L1 mAb to enhance efficacy. Although combinations with targeted agents or other checkpoint mAbs have yielded enhanced clinical outcomes in multiple clinical trials nowadays, the potential of PD-1/PD-L1 mAbs still has a large development space. More potential mechanisms that affect the efficacy demand to be developed and transformed into the clinical treatment of aRCC to search for possible combination regimens. We elucidate these mechanisms in RCC and present existing combination therapies applied in clinical trials. This may help physicians' select treatment options for patients with refractory kidney cancer.
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http://dx.doi.org/10.1002/cam4.4190DOI Listing
September 2021

Optimization Strategy to Inhibit Droplets Rebound on Pathogen-Modified Hydrophobic Surfaces.

ACS Appl Mater Interfaces 2021 Aug 10;13(32):38018-38028. Epub 2021 Aug 10.

Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Tai'an, Shandong 271018, People's Republic of China.

The deposition and retention of pesticide sprays on the surface of hydrophobic plant leaves is a major agricultural challenge, and the deposition of hydrophobic surfaces caused by plant leaf diseases is also a major agricultural problem. Many recent studies have focused on evaluating the effect of adding surfactants to water rather than to pesticide solutions to increase the deposition and retention of spray liquids. Here, we report a strategy to solve the problem of deposition and retention by studying the impact of the behavior of pesticide droplets with added surfactants and performing kinetic analysis on cucumber leaves with powdery mildew. The reduction in the bounce and splash of the pesticide droplets was analyzed by combining the pinning site formed in the retraction stage and the viscous dissipation in the rebound stage. In the practical application of the pesticide spray, we can clearly see that the bounce, splash, and powdery mildew spore ejection decreased when surfactants were added to the pesticide spray that was used on the cucumber leaves, and the adhesion and retention increased. The proposed comprehensive method is helpful for understanding the interactions between pesticide spray droplets and the surface of cucumber leaves with powdery mildew.
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http://dx.doi.org/10.1021/acsami.1c07109DOI Listing
August 2021

Follicular Helper T Cells Remodel the Immune Microenvironment of Pancreatic Cancer via Secreting CXCL13 and IL-21.

Cancers (Basel) 2021 Jul 22;13(15). Epub 2021 Jul 22.

Department of Pancreatic Surgery, Shanghai Cancer Center, Fudan University, Shanghai 200032, China.

Immunosuppression is an important factor for the poor prognosis of pancreatic ductal adenocarcinoma (PDAC). Follicular helper T cells (Tfh cells) play an anti-tumor role in various malignant solid tumors and predict better patient prognosis. In the present study, we aimed to determine the immunosuppressive mechanism associated with Tfh cells and explore a new strategy to improve the tumor microenvironment of PDAC. Flow cytometry was used to detect the infiltration and proportion of Tfh cells in tumor tissues and peripheral blood from patients with PDAC. The spatial correlations of Tfh cells with related immune cells were evaluated using immunofluorescence. The function of Tfh cells was examined using in vitro and in vivo model systems. The high infiltration of Tfh cells predicted better prognosis in patients with PDAC. Tfh cells recruited CD8 T cells and B cells by secreting C-X-C motif chemokine ligand 13 (CXCL13), and promoted the maturation of B cells into antibody-producing plasma cells by secreting interleukin 21 (IL-21), thereby promoting the formation of an immunoactive tumor microenvironment. The function of Tfh cells was inhibited by the programmed cell death 1 ligand 1 (PD-L1)/programmed cell death 1 (PD-1) signaling pathway in PDAC, which could be reversed using neoadjuvant chemotherapy. Treatment with recombinant CXCL13, IL-21 and Tfh cells alleviated tumor growth and enhanced the infiltration of CD8 T cells and B cells, as well as B cell maturation in a PDAC mouse model. Our results revealed the important role of Tfh cells in mediating anti-tumor cellular immunity and humoral immunity in PDAC via secreting CXCL13 and IL-21 and determined a novel mechanism of immunosuppression in PDAC.
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http://dx.doi.org/10.3390/cancers13153678DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8345153PMC
July 2021

Circulating IL-17 Level Is Positively Associated with Disease Activity in Patients with Systemic Lupus Erythematosus: A Systematic Review and Meta-Analysis.

Biomed Res Int 2021 21;2021:9952463. Epub 2021 Jul 21.

Faculty of Nursing, Chiang Mai University, Chiangmai, Thailand.

Previous studies on the relationship between the circulating level of interleukin-17 (IL-17) and disease activity in systemic lupus erythematosus (SLE) were contradictory. This study is aimed at quantitatively assessing the correlation between the circulating IL-17 level and disease activity in SLE patients. A systematic search for related literature was conducted via PubMed, Web of Science, EMBASE, and Cochrane Library (up to January 26, 2021). The relationship between circulating IL-17 levels and SLE activity was evaluated using Fisher's value, which was then converted to . The standardized mean difference (SMD) and its 95% confidence interval (CI) were used to describe the difference between the circulating IL-17 level in patients with active and inactive SLE. STATA 16.0 was used to perform statistical analysis. Random-effects model was performed to synthesize data. Twenty-six studies involving 1,560 SLE patients were included in this review. The pooled value was 0.38 (95% CI: 0.25-0.50; = 83.8%, < 0.001) between the SLE activity and circulating level of IL-17. Patients with active SLE had higher level of circulating IL-17 than that of inactive (pooled SMD = 0.95, 95% CI: 0.38-1.53; = 90.5%, < 0.001). The subgroup analysis suggested that the region and detection method of circulating IL-17 might not be a source of heterogeneity. No significant publication bias was found. In summary, circulating IL-17 level has a low positive relationship with SLE activity. It is necessary to carefully consider the use of circulating IL-17 as a biomarker of the disease activity in SLE patients. The relationship between the circulating level of IL-17 and SLE activity should be further confirmed in randomized controlled studies.
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http://dx.doi.org/10.1155/2021/9952463DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8318742PMC
July 2021

Culprit vascular patterns and surgical outcomes of hemifacial spasm caused by an AICA segment passing between cranial nerve VII and VIII: A series of 25 cases.

Clin Neurol Neurosurg 2021 08 24;207:106777. Epub 2021 Jun 24.

Department of Neurosurgery, West China Hospital of Sichuan University, Chengdu, China. Electronic address:

Objective: To report the vascular anatomic characteristics and surgical outcomes of hemifacial spasm (HFS) caused by an anterior inferior cerebellar artery (AICA) segment passing between cranial nerve VII (CN VII) and cranial nerve VIII (CN VIII).

Patients And Methods: This case series study retrospectively reviewed records of 1040 consecutive patients treated with MVD for HFS in our hospital in 10 years. 25 patients had the culprit vessel recorded as an AICA segment passing between CN VII and CN VIII. Vascular anatomic characteristics were reviewed from intraoperative microscopic videos. The clinical outcomes were followed up at 3-month and 1-year time points.

Results: The culprit AICA segments feature 3 discrete anatomic patterns. The patterns denoted as pattern A, B, and C were identified in 19(76%), 3(12%), and 3 (12%) of the 25 patients respectively. Postoperative spasm relief were achieved in 19(76%), 22(88%), and 23 (92%) of the patients at immediately after surgery, 3-month, and 1-year follow-up respectively. 3(12%) of them have permanent postoperative cranial nerve deficits, including one patient with hearing loss and 2 patients with vocal cord palsy.

Conclusions: Though an AICA segment passing between CN VII and CN VIII is common, very rarely it was deemed the culprit for HFS in our patients. We used fREZ centered definition and operation. We found the culprit AICA segments feature 3 discrete anatomic patterns. We observed good spasm relief outcome and relatively fewer complications with CN VII and CN VIII. Identifying the 3 anatomic patterns may help with a smooth decision-making when vascular compression by an AICA segment passing between CN VII and CN VIII is suspected.
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http://dx.doi.org/10.1016/j.clineuro.2021.106777DOI Listing
August 2021

The combination of N-ammonia and C-methionine in differentiation of residual/recurrent pituitary adenoma from the pituitary gland remnant after trans-sphenoidal Adenomectomy.

BMC Cancer 2021 Jul 20;21(1):837. Epub 2021 Jul 20.

Department of Medical Imaging, The First Affiliated Hospital, Sun Yat-sen University, 58#, Zhongshan Er Road, Guangzhou, Guangdong Province, 510080, People's Republic of China.

Background: This study aimed to assess the clinical usefulness of N-ammonia and C- Methionine (MET) positron emission tomography (PET)/ computed tomography (CT) in the differentiation of residual/recurrent pituitary adenoma (RPA) from the pituitary gland remnant (PGR) after trans-sphenoidal adenomectomy.

Methods: Between June 2012 and December 2019, a total of 19 patients with a history of trans-sphenoidal adenomectomy before PET/CT scans and histological confirmation of RPA after additional surgery in our hospital were enrolled in this study. Images were interpreted by visual evaluation and semi-quantitative analysis. In semi-quantitative analysis, the maximum standard uptake value (SUVmax) of the target and gray matter was measured and the target uptake/gray matter uptake (T/G) ratio was calculated.

Results: The T/G ratios of N-ammonia were significantly higher in PGR than RPA (1.58 ± 0.69 vs 0.63 ± 1.37, P < 0.001), whereas the T/G ratios of C-MET were obviously lower in PGR than RPA (0.78 ± 0.35 vs 2.17 ± 0.54, P < 0.001). Using the canonical discriminant analysis, we calculated the predicted accuracy of RPA (100%), PGR (92.9%), and the overall predicted accuracy (96.43%).

Conclusions: The combination of N-ammonia and C-MET PET/CT is valuable in the differentiation of RPA from PGR after trans-sphenoidal adenomectomy.
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http://dx.doi.org/10.1186/s12885-021-08574-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8290571PMC
July 2021

Impact of surgical margin width on long-term outcomes for intrahepatic cholangiocarcinoma: a multicenter study.

BMC Cancer 2021 Jul 20;21(1):840. Epub 2021 Jul 20.

Department of Hepatobiliary Surgery, Mengchao Hepatobiliary Hospital of Fujian Medical University, Xihong Road 312, Fuzhou, 350025, Fujian, People's Republic of China.

Background: The objective of this study was to investigate the survival outcomes of surgical margin width in intrahepatic cholangiocarcinoma (ICC).

Methods: Between November 2011 and August 2017, patients who underwent hepatectomy for ICC were collected from 13 major hepatopancreatobiliary centers in China. The survival outcomes for patients who underwent wide margin hepatectomy (WMH) were compared with those who underwent narrow margin hepatectomy (NMH) using the 1:1 propensity score matching (PSM).

Results: Among 478 included patients, 195 (40.8%) underwent WMH whereas 283 (59.2%) underwent NMH. PSM yielded 79 matched patients with similar baseline characteristics. Patients underwent WMH had a significant better OS and DFS compared with those underwent NMH (before PSM: median OS 27 vs 17 months, P < 0.05; median DFS 15 vs 8 months, P = 0.001, after PSM: median OS 41 vs 22 months, p < 0.05; median DFS 16 vs 10 months, p < 0.05). However, subgroup analysis based on the AJCC staging system, WMH could only improve the survival outcomes in AJCC I ICC patients (Stage I: OS, DFS, P<0.05).

Conclusions: Surgeons should strive to achieve a wide surgical margin for patients with AJCC I ICC to optimize the long-term outcome.
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http://dx.doi.org/10.1186/s12885-021-08560-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8293518PMC
July 2021

Claudin-7 deficiency promotes stemness properties in colorectal cancer through Sox9-mediated Wnt/β-catenin signalling.

J Transl Med 2021 07 19;19(1):311. Epub 2021 Jul 19.

Department of Oncology, Beijing Shijitan Hospital, Capital Medical University, Beijing, 100038, China.

Background: Colorectal cancer (CRC) is a common malignant tumour of the digestive tract that is characterized by high patient morbidity and mortality rates. Claudin-7 (Cldn7), a tight junction protein, was recently reported to function as a candidate tumour suppressor gene in CRC. Our previous study demonstrated that the large intestine of C57/BL6 mice showed intestinal adenomas and abnormal Ki67 expression and distribution in the intestinal crypt when Cldn7 was knocked out. The aim of this study was to further investigate whether Cldn7 deficiency has non-tight junction functions, affects intestinal stemness properties, promotes CRC and to determine the specific mechanism.

Methods: Cell proliferation assays, migration assays, apoptosis assays, tumour sphere formation assays in vitro, and subcutaneous xenograft models in vivo were used to determine the effects of Cldn7 knockdown on the biological characteristics of CRC stem cells. Western blotting, qPCR and immunofluorescence staining were performed to identify the epithelial-mesenchymal transition and the activation of Wnt/β-catenin pathway in CRC stem cells. Cldn7 inducible conditional gene knockout mice and immunohistochemical staining further verified this hypothesis in vivo. The mechanism and target of Cldn7 were determined by performing a chromatin immunoprecipitation (ChIP) assay and coimmunoprecipitation (CoIP) assay.

Results: Cldn7 knock down in CRC stem cells promoted cell proliferation, migration, and globular growth in serum-free medium and the ability to form xenograft tumours; cell apoptosis was inhibited, while the cellular epithelial-mesenchymal transition was also observed. These changes in cell characteristics were achieved by activating the Wnt/β-catenin pathway and promoting the expression of downstream target genes after β-catenin entry into the nucleus, as observed in CRC cell lines and Cldn7 gene knockout mouse experiments. Using ChIP and CoIP experiments, we initially found that Cldn7 and Sox9 interacted at the protein level to activate the Wnt/β-catenin pathway.

Conclusions: Based on our research, Cldn7 deficiency confers stemness properties in CRC through Sox9-mediated Wnt/β-catenin signalling. This result clarifies that Cldn7 plays an inhibitory role in CRC and reveals a possible molecular mechanism, which is conducive to further research on Cldn7 and cancer stem cells.
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http://dx.doi.org/10.1186/s12967-021-02983-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8287764PMC
July 2021

Two chemoattenuated PfSPZ malaria vaccines induce sterile hepatic immunity.

Nature 2021 07 30;595(7866):289-294. Epub 2021 Jun 30.

Sanaria, Rockville, MD, USA.

The global decline in malaria has stalled, emphasizing the need for vaccines that induce durable sterilizing immunity. Here we optimized regimens for chemoprophylaxis vaccination (CVac), for which aseptic, purified, cryopreserved, infectious Plasmodium falciparum sporozoites (PfSPZ) were inoculated under prophylactic cover with pyrimethamine (PYR) (Sanaria PfSPZ-CVac(PYR)) or chloroquine (CQ) (PfSPZ-CVac(CQ))-which kill liver-stage and blood-stage parasites, respectively-and we assessed vaccine efficacy against homologous (that is, the same strain as the vaccine) and heterologous (a different strain) controlled human malaria infection (CHMI) three months after immunization ( https://clinicaltrials.gov/ , NCT02511054 and NCT03083847). We report that a fourfold increase in the dose of PfSPZ-CVac(PYR) from 5.12 × 10 to 2 × 10 PfSPZs transformed a minimal vaccine efficacy (low dose, two out of nine (22.2%) participants protected against homologous CHMI), to a high-level vaccine efficacy with seven out of eight (87.5%) individuals protected against homologous and seven out of nine (77.8%) protected against heterologous CHMI. Increased protection was associated with Vδ2 γδ T cell and antibody responses. At the higher dose, PfSPZ-CVac(CQ) protected six out of six (100%) participants against heterologous CHMI three months after immunization. All homologous (four out of four) and heterologous (eight out of eight) infectivity control participants showed parasitaemia. PfSPZ-CVac(CQ) and PfSPZ-CVac(PYR) induced a durable, sterile vaccine efficacy against a heterologous South American strain of P. falciparum, which has a genome and predicted CD8 T cell immunome that differs more strongly from the African vaccine strain than other analysed African P. falciparum strains.
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http://dx.doi.org/10.1038/s41586-021-03684-zDOI Listing
July 2021

Thicker endometrium on hCG trigger day improves the live birth rate of fresh cleavage embryo transfer in GnRH-agonist regimen of normogonadotrophic women.

Ann Transl Med 2021 May;9(10):856

Department of Reproductive Medicine, the First People's Hospital of Yunnan Province, Kunming, China.

Background: Luteinizing hormone (LH) and progesterone (PROG) on human chorionic gonadotropin (hCG) trigger day are significantly correlated with assisted reproductive technology (ART) outcome. Moreover, LH and PROG are also involved in the functional preparation of the endometrium during the implantation window; however, whether they are related to endometrial thickness (EMT) is still unknown. The aim of the present study was to assess whether EMT has a positive correlation on the live birth rate following fresh embryo transfer (ET), and whether LH and PROG have an impact on EMT.

Methods: A total of 2,260 normogonadotrophic women were treated with a GnRH agonist for in vitro fertilization (IVF)/intracytoplasmic sperm injection. Patients with advanced age and poor ovarian reserve were excluded. The levels of LH, PROG, and EMT on the hCG trigger day were divided into binary variables, respectively, by the cutoff values, and which were obtained based on receiver operating characteristic curve analysis of live birth among LH, PROG and EMT levels on the hCG trigger day, respectively. Multivariate binary logistic regression was used to confirm the role of LH, PROG, and EMT on the live birth, and stratified analysis was used to determine whether LH and PROG have an impact on EMT.

Results: EMT and LH were protective factors for live births, with odds ratios (OR) of 1.11 [95% confidence interval (CI): 1.066-1.157] and 1.696 (95% CI: 1.345-2.139), respectively. However, PROG was a risk factor for live birth, with an OR of 0.635 (95% CI: 0.526-0.766). The hierarchical cross-table analysis indicated that EMT had no significant difference for live birth in the combination of low LH and high PROG group. In the other subgroups, thick EMT was associated with a higher live birth rate (P<0.05).

Conclusions: On hCG trigger day, EMT, LH, and PROG all were independent factors that affected the live birth of fresh ETs. Thick EMT can significantly increase the live birth rate. However, multivariate logistic regression analysis showed that EMT does not affect the live birth rate in combination of low LH and high PROG environment.
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http://dx.doi.org/10.21037/atm-21-1922DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8184494PMC
May 2021

Adipsin promotes bone marrow adiposity by priming mesenchymal stem cells.

Elife 2021 06 22;10. Epub 2021 Jun 22.

Naomi Berrie Diabetes Cente, Columbia University, New York, United States.

Background: Marrow adipose tissue (MAT) has been shown to be vital for regulating metabolism and maintaining skeletal homeostasis in the bone marrow (BM) niche. As a reflection of BM remodeling, MAT is highly responsive to nutrient fluctuations, hormonal changes, and metabolic disturbances such as obesity and diabetes mellitus. Expansion of MAT has also been strongly associated with bone loss in mice and humans. However, the regulation of BM plasticity remains poorly understood, as does the mechanism that links changes in marrow adiposity with bone remodeling.

Methods: We studied deletion of Adipsin, and its downstream effector, C3, in C57BL/6 mice as well as the bone-protected PPARγ constitutive deacetylation 2KR mice to assess BM plasticity. The mice were challenged with thiazolidinedione treatment, calorie restriction, or aging to induce bone loss and MAT expansion. Analysis of bone mineral density and marrow adiposity was performed using a μCT scanner and by RNA analysis to assess adipocyte and osteoblast markers. For studies, primary bone marrow stromal cells were isolated and subjected to osteoblastogenic or adipogenic differentiation or chemical treatment followed by morphological and molecular analyses. Clinical data was obtained from samples of a previous clinical trial of fasting and high-calorie diet in healthy human volunteers.

Results: We show that Adipsin is the most upregulated adipokine during MAT expansion in mice and humans in a PPARγ acetylation-dependent manner. Genetic ablation of Adipsin in mice specifically inhibited MAT expansion but not peripheral adipose depots, and improved bone mass during calorie restriction, thiazolidinedione treatment, and aging. These effects were mediated through its downstream effector, complement component C3, to prime common progenitor cells toward adipogenesis rather than osteoblastogenesis through inhibiting Wnt/β-catenin signaling.

Conclusions: Adipsin promotes new adipocyte formation and affects skeletal remodeling in the BM niche. Our study reveals a novel mechanism whereby the BM sustains its own plasticity through paracrine and endocrine actions of a unique adipokine.

Funding: This work was supported by the National Institutes of Health T32DK007328 (NA), F31DK124926 (NA), R01DK121140 (JCL), R01AR068970 (BZ), R01AR071463 (BZ), R01DK112943 (LQ), R24DK092759 (CJR), and P01HL087123 (LQ).
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http://dx.doi.org/10.7554/eLife.69209DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8219379PMC
June 2021

Correcting physiological noise in whole-head functional near-infrared spectroscopy.

J Neurosci Methods 2021 08 17;360:109262. Epub 2021 Jun 17.

Stephenson School of Biomedical Engineering, University of Oklahoma, Norman, OK, USA; Institute for Biomedical Engineering, Science and Technology, University of Oklahoma, Norman, OK, USA. Electronic address:

Background: Functional near-infrared spectroscopy (fNIRS) has been increasingly employed to monitor cerebral hemodynamics in normal and diseased conditions. However, fNIRS suffers from its susceptibility to superficial activity and systemic physiological noise. The objective of the study was to establish a noise reduction method for fNIRS in a whole-head montage.

New Method: We have developed an automated denoising method for whole-head fNIRS. A high-density montage consisting of 109 long-separation channels and 8 short-separation channels was used for recording. Auxiliary sensors were also used to measure motion, respiration and pulse simultaneously. The method incorporates principal component analysis and general linear model to identify and remove a globally uniform superficial component. Our denoising method was evaluated in experimental data acquired from a group of healthy human subjects during a visually cued motor task and further compared with a minimal preprocessing method and three established denoising methods in the literature. Quantitative metrics including contrast-to-noise ratio, within-subject standard deviation and adjusted coefficient of determination were evaluated.

Results: After denoising, whole-head topography of fNIRS revealed focal activations concurrently in the primary motor and visual areas.

Comparison With Existing Methods: Analysis showed that our method improves upon the four established preprocessing methods in the literature.

Conclusions: An automatic, effective and robust preprocessing pipeline was established for removing physiological noise in whole-head fNIRS recordings. Our method can enable fNIRS as a reliable tool in monitoring large-scale, network-level brain activities for clinical uses.
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http://dx.doi.org/10.1016/j.jneumeth.2021.109262DOI Listing
August 2021

Computational design and synthesis of molecular imprinted polymers for selective solid phase extraction of sulfonylurea herbicides.

J Chromatogr A 2021 Aug 3;1651:462321. Epub 2021 Jun 3.

Technical Center for Animal Plant and Food Inspection and Quarantine of Shanghai Customs, Shanghai 200135, China.

A high-efficiency approach for the synthesis of molecularly imprinted polymers has been developed and further for the solid-phase extraction of sulfonylurea herbicides in food samples. Molecular simulation approach combined chemometric selected metsulfuron-methyl (MSM) and 2-trifluoromethyl acrylic acid (TFMAA) as the template and the monomer to synthesize the molecularly imprinted polymers (MIPs). Experimental validation confirmed that the MSM-imprinted polymers showed a higher selectivity and affinity to sulfonylurea herbicides. The optimized molecularly imprinted solid-phase extraction (MISPE) conditions, including loading, washing, and eluting conditions, were established. The developed MISPE technology combined HPLC-MSMS was successfully used for the determination of sulfonylurea herbicides in foods. Compared with commercial SPE columns, MISPE showed high affinity, excellent selectivity and low matrix effect. The recoveries of sulfonylurea herbicides spiked in four matrices were between 86.4% and 100.2%, with the relative standard deviations (RSD) in the range of 0.9%-10.5%.
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http://dx.doi.org/10.1016/j.chroma.2021.462321DOI Listing
August 2021

Analysis of risk factors for early stent thrombosis in the Chinese population: A multicenter restrospective study.

World J Emerg Med 2021 ;12(3):192-197

Department of Cardiology, General Hospital of Northern Theatre Command, Shenyang 110016, China.

Background: The predictive scoring systems for early stent thrombosis (EST) remains blank in China. The study aims to evaluate the risk factors and conduct a prediction model of EST in the Chinese population.

Methods: EST was defined as thrombosis that occurs within the first 30 days after primary percutaneous coronary intervention (PCI). Patients from ten Chinese hospitals diagnosed as stent thrombosis (ST) from January 2010 to December 2016 were retrospectively included as the study group. A control group (1 case:2 controls) was created by including patients without ST, major adverse cardiovascular events, or cerebrovascular events during follow-up. The present study evaluated 426 patients with single-vessel lesions and ultimately included 40 patients with EST and 80 control patients, who were included to identify factors that predicted EST and to develop a prediction scoring system. The other 171 patients without integrated 1:2 pair were used for external validation.

Results: EST was independently associated with a low hemoglobin concentration (adjusted odds ratio [] 0.946, 95% confidence interval [95% ] 0.901-0.993, =0.026), a high pre-PCI Synergy between Percutaneous Coronary Intervention with Taxus and Cardiac Surgery (SYNTAX) score ( 1.166, 95% 1.049-1.297, =0.004), and a DAPT (DAPT) duration of <30 days ( 28.033, 95% 5.302-272.834, <0.001). The simple EST prediction score provided an area under the curve (AUC) of 0.854 (95% 0.777-0.932, <0.001) with 70.0% sensitivity and 90.0% specificity, and 0.742 (95% 0.649-0.835, <0.001) with 54.5% sensitivity and 81.0% specificity for external validation dataset.

Conclusions: EST may be independently associated with DAPT discontinuation within 30 days, a low hemoglobin concentration, and a high SYNTAX score. The scoring system also has a good ability to predict the risk of EST and may be useful in the clinical setting.
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http://dx.doi.org/10.5847/wjem.j.1920-8642.2021.03.005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8188284PMC
January 2021

Combined hepatocellular carcinoma-cholangiocarcinoma: A misdiagnosed case preoperatively.

JGH Open 2021 Jun 20;5(6):720-721. Epub 2021 May 20.

Department of General Surgery Affiliated Xiaoshan Hospital, Hangzhou Normal University Hangzhou China.

Combined hepatocellular carcinoma-cholangiocarcinoma (cHCC-CC) is a rare subtype in primary liver cancer, which is difficult to diagnose in clinics. In this report, we present a case of a 62-year-old male with abdominal pain and slightly elevated alpha-fetoprotein. The contrast-enhanced computed tomography scans showed a left liver mass, which showed adherence to the imaging characteristics of hepatocellular carcinoma. However, with the treatment of laparoscopic liver resection, the following histological examination displayed two distinct components, which were consistent with the diagnosis of cHCC-CC.
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http://dx.doi.org/10.1002/jgh3.12574DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8171155PMC
June 2021

Structural and functional reorganization of contralateral hippocampus after temporal lobe epilepsy surgery.

Neuroimage Clin 2021 2;31:102714. Epub 2021 Jun 2.

Department of Neurology, West China Hospital, Sichuan University, Chengdu, Sichuan, China. Electronic address:

Objective: To explore the structural and functional reorganization of contralateral hippocampus in patients with unilateral mesial temporal lobe epilepsy (mTLE) who achieved seizure-freedom after anterior temporal lobectomy (ATL).

Methods: We obtained high-resolution structural MRI and resting-state functional MRI data in 28 unilateral mTLE patients and 29 healthy controls. Patients were scanned before and three and 24 months after surgery while controls were scanned only once. Hippocampal gray matter volume (GMV) and functional connectivity (FC) were assessed.

Results: No obvious GMV changes were observed in contralateral hippocampus before and after successful surgery. Before surgery, ipsilateral hippocampus showed increased FC with ipsilateral insula (INS) and temporoparietal junction (TPJ), but decreased FC with widespread bilateral regions, as well as contralateral hippocampus. After successful ATL, contralateral hippocampus showed: (1) decreased FC with ipsilateral INS at three months follow-up, without further changes; (2) decreased FC with ipsilateral TPJ, postcentral gyrus and rolandic operculum at three months, with an obvious increase at 24 months follow-up; (3) increased FC with bilateral medial prefrontal cortex (MPFC) and superior frontal gyrus (SFG) at three months follow-up, without further changes.

Conclusions: Successful ATL may not lead to an obvious structural reorganization in contralateral hippocampus. Surgical manipulation may lead to a transient FC reduction of contralateral hippocampus. Increased FC between contralateral hippocampus and bilateral MPFC and SFG may be related to postoperative functional remodeling.
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http://dx.doi.org/10.1016/j.nicl.2021.102714DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8187253PMC
September 2021

MicroRNA-486-5p Suppresses Lung Cancer Downregulating mTOR Signaling and .

Front Oncol 2021 20;11:655236. Epub 2021 May 20.

Lab for Noncoding RNA & Cancer, School of Life Sciences, Shanghai University, Shanghai, China.

Lung cancer is one of the central causes of tumor-related deaths globally, of which non-small cell lung cancer (NSCLC) takes up about 85%. As key regulators of various biological processes, microRNAs (miRNAs) have been verified as crucial factors in NSCLC. To elucidate the role of miR-486-5p in the mTOR pathway, we investigated its role in NSCLC and related signaling. Our results confirmed that miR-486-5p was downregulated in most of human NSCLC tissue samples and cell lines. Further study confirmed that it inhibited NSCLC through repression of the mTOR pathway targeting both ribosomal proteins S6 kinase A1 (RPS6KA1, RSK) and ribosomal proteins S6 kinase B1 (RPS6KB1, p70S6K), which are critical components of the mTOR signaling. Additionally, miR-486-5p impeded tumor growth and inhibited tumor metastasis through repression of the epithelial-mesenchymal transition (EMT). Taken together, our study verified the role that miR-486-5p exerts in NSCLC, and its expression pattern in the different stages and morphologies of NSCLC makes it a promising biomarker in the early diagnosis of the disease.
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http://dx.doi.org/10.3389/fonc.2021.655236DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8172781PMC
May 2021

Controllable Compositions and Structures of [email protected]@C-Ni Hybrids with a Silica Layer as a Mineral Redox Buffer.

Inorg Chem 2021 Jun 27;60(12):8880-8889. Epub 2021 May 27.

College of Chemistry and Chemical Engineering, Shanghai University of Engineering Science, Shanghai 201620, China.

Mineral redox buffer is a vital concept in geology that can be applied to modulate hybrid compositions and generate nanostructures with expected morphology. Here, combining a dual coating of an inorganic silica and organic resorcinol-formaldehyde-Ni (RF-Ni) layer on α-FeO spindles with a subsequent calcination process, core-shell [email protected]@C-Ni composites with multicompositional structures were fabricated as efficient catalysts for 4-nitrophenol (4-NP) reduction. Notably, the silica layer as a redox buffer between hematite cores and the RF-Ni shell played a crucial role in modulating the compositions and structures of the [email protected]@C-Ni. Without the silica layer, FeO-Ni/C composites with Ni nanoparticles trapped into the FeO cores were generated. Moreover, a significant impact of the calcination temperature on morphologies and compositions of the [email protected]@C-Ni catalysts along with their catalytic performances has been verified. As a result, the catalyst annealed at 500 °C exhibited a high magnetic property and optimized morphology with high-density small nickel nanoparticles (∼11.6 nm), showing remarkably enhanced catalytic activity compared to the FeO-Ni/C composites and excellent recyclability with a high conservation of about 92%. Furthermore, this synthetic strategy shows significant potential to modulate the nanostructures and phases of other multivalent metal oxide nanocomposites.
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http://dx.doi.org/10.1021/acs.inorgchem.1c00778DOI Listing
June 2021
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