Publications by authors named "Ding Chen"

365 Publications

Establishment of an Immune Cell Infiltration Score to Help Predict the Prognosis and Chemotherapy Responsiveness of Gastric Cancer Patients.

Front Oncol 2021 9;11:650673. Epub 2021 Jul 9.

Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai, China.

The immune microenvironment plays a critical role in tumor biology. The molecular profiles of immune components and related genes are of tremendous value for the study of primary resistance to immune checkpoint blockers (ICBs) for gastric cancer (GC) and serve as prognostic biomarkers to predict GC survival. Recent studies have revealed that tumor immune cell infiltration (ICI) is an indicator of the survival and responsiveness to chemotherapy in GC patients. Here, we describe the immune cell landscape based on the ESTIMATE and CIBERSORT algorithms to help separate GC into 3 ICI clusters using the unsupervised clustering method. Further in-depth analyses, such as differential expression gene (DEG) analysis and principal component analysis (PCA), help to establish an ICI scoring system. A low ICI score is characterized by an increased tumor mutation burden (TMB). The combination of the ICI score and TMB score better predicts the survival of GC patients. Analyses based on public and our own database revealed that the ICI scoring system could also help predict the survival and chemotherapy responsiveness of GC patients. The present study demonstrated that the ICI score may be an effective prognostic biomarker and predictive indicator for chemotherapy and immunotherapy.
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http://dx.doi.org/10.3389/fonc.2021.650673DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8299334PMC
July 2021

News recommender system: a review of recent progress, challenges, and opportunities.

Artif Intell Rev 2021 Jul 21:1-52. Epub 2021 Jul 21.

Ryerson University, Toronto, Canada.

Nowadays, more and more news readers read news online where they have access to millions of news articles from multiple sources. In order to help users find the right and relevant content, news recommender systems (NRS) are developed to relieve the information overload problem and suggest news items that might be of interest for the news readers. In this paper, we highlight the major challenges faced by the NRS and identify the possible solutions from the state-of-the-art. Our discussion is divided into two parts. In the first part, we present an overview of the recommendation solutions, datasets, evaluation criteria beyond accuracy and recommendation platforms being used in the NRS. We also talk about two popular classes of models that have been successfully used in recent years. In the second part, we focus on the deep neural networks as solutions to build the NRS. Different from previous surveys, we study the effects of news recommendations on user behaviors and try to suggest possible remedies to mitigate those effects. By providing the state-of-the-art knowledge, this survey can help researchers and professional practitioners have a better understanding of the recent developments in news recommendation algorithms. In addition, this survey sheds light on the potential new directions.
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http://dx.doi.org/10.1007/s10462-021-10043-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8294232PMC
July 2021

Preliminary results in anterior cervical discectomy and fusion with the uncovertebral joint fusion cage in a goat model.

BMC Musculoskelet Disord 2021 Jul 17;22(1):628. Epub 2021 Jul 17.

Department of Operation Room and Anesthesia Center, West China Hospital, Sichuan University, No. 37 Guo Xue Rd, Chengdu, 610041, China.

Objective: To preliminarily evaluate the safety and efficacy of the uncovertebral joint fusion cage in a goat model of cervical spine interbody fusion.

Methods: Twenty-four healthy adult goats were randomly assigned to one of the two following groups: Group A, goats were implanted with an uncovertebral joint fusion cage combined with a local autograft and Group B, goats were implanted with a non-profile cage filled with a local autograft. The goats were prospectively evaluated for 24 weeks and then were sacrificed for evaluation. X-rays, CT and micro-CT scanning, and undecalcified bone histological analysis were used for the evaluation of fusion.

Results: 75.0% (9/12) of the goats in Group A were evaluated as having fusion at 12 weeks, compared to 41.7% (5/12) in Group B. 83.3% (10/12) of the goats in Group A were evaluated as having fusion at 24 weeks compared to 58.3% (7/12) in Group B. The fusion grading scores in Group A were significantly higher than that in Group B both at 12 weeks and 24 weeks (P < 0.05). Micro-CT scanning and undecalcified bone histological analysis showed that new bone formation can be obviously found in the bilateral uncovertebral joint. The bone volume fraction (BV/ TV) in Group A (23.59 ± 4.43%) was significantly higher than Group B (16.16 ± 4.21%), with P < 0.05.

Conclusions: Preliminary results of this study demonstrated that uncovertebral joint fusion cage is effective for achieving early bone formation and fusion without increase of serious complications.
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http://dx.doi.org/10.1186/s12891-021-04412-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8286593PMC
July 2021

Risk factors and biofilm formation analyses of hospital-acquired infection of Candida pelliculosa in a neonatal intensive care unit.

BMC Infect Dis 2021 Jun 29;21(1):620. Epub 2021 Jun 29.

Department of Laboratory Medicine of Shengjing Hospital of China Medical University, 36 Sanhao Street, Heping District, Shenyang, China.

Background: Candida pelliculosa is an ecological fungal species that can cause infections in immunocompromised individuals. Numerous studies globally have shown that C. pelliculosa infects neonates. An outbreak recently occurred in our neonatal intensive care unit; therefore, we aimed to evaluate the risk factors in this hospital-acquired fungal infection.

Methods: We performed a case-control study, analysing the potential risk factors for neonatal infections of C. pelliculosa so that infection prevention and control could be implemented in our units. Isolated strains were tested for drug resistance and biofilm formation, important factors for fungal transmission that give rise to hospital-acquired infections.

Results: The use of three or more broad-spectrum antimicrobials or long hospital stays were associated with higher likelihoods of infection with C. pelliculosa. The fungus was not identified on the hands of healthcare workers or in the environment. All fungal isolates were susceptible to anti-fungal medications, and after anti-fungal treatment, all infected patients recovered. Strict infection prevention and control procedures efficiently suppressed infection transmission. Intact adhesin-encoding genes, shown by genome analysis, indicated possible routes for fungal transmission.

Conclusions: The use of three or more broad-spectrum antimicrobials or a lengthy hospital stay is theoretically associated with the risk of infection with C. pelliculosa. Strains that we isolated are susceptible to anti-fungal medications, and these were eliminated by treating all patients with an antifungal. Transmission is likely via adhesion to the cell surface and biofilm formation.
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http://dx.doi.org/10.1186/s12879-021-06295-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8244135PMC
June 2021

Deep Dive on the Proteome of Human Body Fluids: A Valuable Data Resource for Biomarker Discovery.

Cancer Genomics Proteomics 2021 Jul-Aug;18(4):549-568

State Key Laboratory of Genetic Engineering and Collaborative Innovation Center for Genetics and Development, School of Life Sciences, Institute of Biomedical Sciences, Human Phenome Institute, Fudan University, Shanghai, P.R. China;

Background/aim: Body fluids are considered to be a rich source of disease biomarkers. Proteins in many body fluids have potential clinical applications for disease diagnostic and prognostic purposes. The aim of this study was to establish an in-depth multi-body fluid proteome.

Materials And Methods: Ten body fluids associated with 8 types of cancers collected from 23 patients involved in 19 common diseases underwent liquid chromatography tandem mass spectrometry (MS) analysis after gel-based protein separation (SDS-PAGE) or peptide-based fractionations. Bioinformatic analysis, including principal component analysis (PCA), consensus clustering, and hierarchical clustering analysis were also performed. The biological function was determined using the Database for Annotation, Visualization and Integrated Discovery (DAVID).

Results: We profiled the proteome of ten body fluids, including ascites, bile, cerebrospinal fluid (CSF), hydrothorax, knee joint fluid (KJF), plasma, saliva, serum, tears, and urine. A total of 3,396 nonredundant proteins were identified, of which 304 were shared among ten body fluids, with common functions in focal adhesion and complement/coagulation cascades. A total of 41.5% (1,409) of the proteins were detected in only one body fluid and were closely related to their adjacent tissues by function. The functional analysis of the remaining 1,683 proteins showed that similar functions might be shared among different body fluids, which further highlighted the close connection of body fluids in the human body.

Conclusion: A deep proteome of multi-body fluids originated from patients diagnosed with 19 common diseases provides a valuable data resource, and might indicate the potential application of body fluids for biomarker discovery.
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http://dx.doi.org/10.21873/cgp.20280DOI Listing
March 2021

Assessment of the self-reported dysphagia in patients undergoing one-level versus two-level cervical disc replacement with the Prestige-LP prosthesis.

Clin Neurol Neurosurg 2021 Jun 11;207:106759. Epub 2021 Jun 11.

Department of Orthopedic Surgery, West China Hospital, Sichuan University, China.

Objectives: Cervical disc replacement (CDR) has been established as an alternative to anterior cervical discectomy and fusion (ACDF) for treating cervical degenerative pathologies over the past decade. However, swallowing difficulties challenge patient safety due to the increased risk of malnutrition, dehydration and aspiration pneumonia after CDR. Currently, there are limited studies focusing on the incidences, severity and associated risk factors for dysphagia after CDR. This paucity, therefore, necessitated this retrospective study on post-operative dysphagia after one- and two-level CDR.

Patients And Methods: One hundred and fourteen patients underwent one-level CDR and forty eight patients underwent two-level CDR were recruited with a mean follow-up of 21 months (ranging from 14 to 30 months). The prevalence and severity of dysphagia was evaluated by the Bazaz grading system during the follow-up time. Regression analyses were done to identify risk factors associated with post-operative dysphagia after CDR.

Results: The overall dysphagia occurrences in the one- and two-level CDR groups were 17.54% and 35.41% at week one, 12.28% and 25% after one month, 9.65% and 18.75% after three months, 6.14% and 14.58% after six months, 4.39% and 6.25% after one year, and 3.51% and 4.17% at the final follow-up, respectively. The identified risk factors for dysphagia after CDR were advanced age, C4/5 surgery, two-level surgery, dC2-C7 angle ≥ - 5° and ≥ 6 mm changes in the prevertebral soft tissue swelling (dPSTS).

Conclusion: The patients who experienced two-level CDR may have poor swallowing functions in the early post-operative term. However, these patients also can recover well with increasing length of follow-up. In addition, patients with advanced age, C4/5 surgery, dC2-C7 angle ≥ - 5° and ≥ 6 mm changes in dPSTS may prone to occur dysphagia after CDR.
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http://dx.doi.org/10.1016/j.clineuro.2021.106759DOI Listing
June 2021

Anion Modulation of Pt-Group Metals and Electrocatalysis Applications.

Chemistry 2021 Jun 15. Epub 2021 Jun 15.

State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan, 430070, P. R. China.

Pt-group metal (PGM) electrocatalysts with unique electronic structures and irreplaceable comprehensive properties play crucial roles in electrocatalysis. Anion engineering can create a series of PGM compounds (such as RuP , IrP , PtP , RuB , Ru B , RuS , etc.) that provide a promising prospect for improving the electrocatalytic performance and use of Pt-group noble metals. This review seeks the electrochemical activity origin of anion-modulated PGM compounds, and systematically analyzes and summarizes their synthetic strategies and energy-relevant applications in electrocatalysis. Orientation towards the sustainable development of nonfossil resources has stimulated a blossoming interest in the design of advanced electrocatalysts for clean energy conversion. The anion-modulated strategy for Pt-group metals (PGMs) by means of anion engineering possesses high flexibility to regulate the electronic structure, providing a promising prospect for constructing electrocatalysts with superior activity and stability to satisfy a future green electrochemical energy conversion system. Based on the previous work of our group and others, this review summarizes the up-to-date progress on anion-modulated PGM compounds (such as RuP , IrP , PtP , RuB , Ru B , RuS , etc.) in energy-related electrocatalysis from the origin of their activity and synthetic strategies to electrochemical applications including hydrogen evolution reaction (HER), oxygen evolution reaction (OER), oxygen reduction reaction (ORR), hydrogen oxidation reaction (HOR), N reduction reaction (NRR), and CO reduction reaction (CO RR). At the end, the key problems, countermeasures and future development orientations of anion-modulated PGM compounds toward electrocatalytic applications are proposed.
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http://dx.doi.org/10.1002/chem.202101645DOI Listing
June 2021

Change in the postoperative intervertebral space height and its impact on clinical and radiological outcomes after ACDF surgery using a zero-profile device: a single-Centre retrospective study of 138 cases.

BMC Musculoskelet Disord 2021 Jun 14;22(1):543. Epub 2021 Jun 14.

Department of Orthopedic Surgery, West China Hospital, Sichuan University, No. 37 Guo Xue Xiang Rd, Chengdu, 610041, Sichuan, China.

Background: The effects of postoperative intervertebral height (IH) changes on the clinical and radiological outcomes after anterior cervical decompression and fusion (ACDF) surgery using a zero-profile device remain unclear.

Methods: We retrospectively reviewed patients who had undergone ACDF using a zero-profile device from March 2012 to February 2016 at our institution. Based on the postoperative IH variation, the patients were divided into group A with postoperative IH 0 to 2 mm, group B with postoperative IH 2 to 4 mm, and group C with postoperative IH greater than 4 mm. Clinical efficacy was evaluated using JOA, VAS, and NDI scores in the groups. Imaging parameters including the IH, cervical lordosis, fusion rate, intervertebral foramen (IVF) diameter and complications such as subsidence, dysphagia, and ASD were also compared across the three groups.

Results: The average IH increased significantly from 6.72 mm preoperatively to 10.46 mm 1 week after surgery, and then gradually decreased to 7.48 mm at the final follow-up. The fusion rate was 61.90% in group A, 63.23% in group B, 53.57% in group C at 3 months, 73.81% in group A, 79.41% in group B, 67.86% in group C at 6 months, 90.48% in group A, 95.59% in group B, 92.86% in group C 1 year after surgery, and at the last follow-up, the fusion rate of three groups was all 100%. The IVF diameter was 6.52 ± 1.80 mm in group A, 9.55 ± 2.36 mm in group B, and 9.34 ± 1.62 mm in group C. ASD at the superior and inferior levels affected 11.90 and 16.67% patients in group A, 5.88 and 7.38% in group B, and 14.28 and 10.71% in group C. Regarding the 3 groups, the subsidence rates were 7.14, 4.41, and 14.29%, respectively.

Conclusions: No clear correlation was found between IH changes and clinical efficacy within a year of surgery. However, the IH may affect various complications after ACDF. If postoperative IH changes are maintained at 2 to 4 mm after a year, a satisfactory imaging parameters and relatively low complications may be achieved after ACDF surgery using a zero-profile device.
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http://dx.doi.org/10.1186/s12891-021-04432-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8204480PMC
June 2021

Quantum-Inspired Support Vector Machine.

IEEE Trans Neural Netw Learn Syst 2021 Jun 10;PP. Epub 2021 Jun 10.

Support vector machine (SVM) is a particularly powerful and flexible supervised learning model that analyzes data for both classification and regression, whose usual algorithm complexity scales polynomially with the dimension of data space and the number of data points. To tackle the big data challenge, a quantum SVM algorithm was proposed, which is claimed to achieve exponential speedup for least squares SVM (LS-SVM). Here, inspired by the quantum SVM algorithm, we present a quantum-inspired classical algorithm for LS-SVM. In our approach, an improved fast sampling technique, namely indirect sampling, is proposed for sampling the kernel matrix and classifying. We first consider the LS-SVM with a linear kernel, and then discuss the generalization of our method to nonlinear kernels. Theoretical analysis shows our algorithm can make classification with arbitrary success probability in logarithmic runtime of both the dimension of data space and the number of data points for low rank, low condition number, and high dimensional data matrix, matching the runtime of the quantum SVM.
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http://dx.doi.org/10.1109/TNNLS.2021.3084467DOI Listing
June 2021

Identification and assessment of pulmonary Cryptococcus neoformans infection by blood serum surface-enhanced Raman spectroscopy.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Nov 20;260:119978. Epub 2021 May 20.

College of Life and Health Sciences, Northeastern University, Shenyang 110169, China. Electronic address:

Cryptococcus neoformans (C. neoformans) is a causative agent for acute pulmonary infection, which can further develop to lethal meningoencephalitis if untreated. The meningoencephalitis infection can be prevented, if timely treatment on pulmonary cryptococcal infection can be implemented based on its early diagnosis and accurate assessment. In this study, blood serum surface-enhanced Raman spectroscopy (SERS) method was investigated on identification and assessment of pulmonary C. neoformans infection. The serum SERS measurements were collected from the mice infected with C. neoformans and the healthy mice, in which the infected mice were further divided into four subgroups according to the duration of infection. Based on those SRES measurements, biochemical differences were analyzed among those different groups to investigate the potential biomarkers for identifying and assessing the pulmonary C. neoformans infection. Furthermore, partial least square (PLS) analysis followed by linear discriminant analysis (LDA) model was employed to identify pulmonary cryptococcal infection and to assess the degrees of infection with the accuracies of 96.7% and 85.3%, respectively. Therefore, our study has demonstrated the great clinical potential of using serum SERS technique for an accurate identification and assessment of pulmonary cryptococcal infection.
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http://dx.doi.org/10.1016/j.saa.2021.119978DOI Listing
November 2021

Demethylation of the SFRP4 Promoter Drives Gastric Cancer Progression via the Wnt pathway.

Mol Cancer Res 2021 May 20. Epub 2021 May 20.

Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai, China.

Wnt signaling is believed to be an important contributor to tumor development and has been reported to be modulated by secreted frizzled-related proteins (SFRP). Nevertheless, the role of secreted frizzled-related protein 4 (SFRP4) in tumorigenesis remains controversial. We aim to explore its biological function in gastric cancer. Genomes analysis based on the Gene Expression Omnibus (GEO) dataset was used to find the differential gene expression between different tumor-node-metastasis (TNM) stages of gastric cancer. IHC was used to determine the relationship between SFRP4 expression and clinicopathologic characteristics in patients with gastric cancer. The influence of SFRP4 on tumor progression was evaluated by CCK-8, colony formation, cell apoptosis, and cell cycle , as well as xenograft model . The methylation status of SFRPs was examined in gastric cancer specimens by quantitative methylation analysis. SFRP4 was most upregulated in advanced gastric cancer. High intratumoral SFRP4 expression, which was associated with tumor invasion and metastasis, was also a poor prognostic indicator for patients with gastric cancer. and studies revealed that SFRP4 could promote tumor growth; however, IWR-1 could suppress tumor growth mediated by SFRP4 overexpression. Mechanistic exploration found that SFRP4 was overexpressed by the decrease of promoter methylation and thus could competitively antagonize the inhibitory effect of SFRP1 on Wnt pathway activation and tumor progression in gastric cancer. IMPLICATIONS: In gastric cancer, the expression of SFRP4 was upregulated by decreased methylation. High intratumoral SFRP4 expression could activate the Wnt pathway to promote tumor progression and predict poor survival of patients with gastric cancer.
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http://dx.doi.org/10.1158/1541-7786.MCR-20-0933DOI Listing
May 2021

Risk factors evaluation of post-transplant lymphoproliferative disorders after allogeneic haematopoietic stem cell transplantation with comparison between paediatric and adult.

J Clin Pathol 2021 May 19. Epub 2021 May 19.

Pathology, Peking University People's Hospital, Beijing, China.

To describe the clincopathological features and evaluate risk factors of post-transplant lymphoproliferative disorder (PTLD) after allogeneic haematopoietic stem cell transplants (allo-HSCT), with comparison between paediatric and adult .Clinicopathological features of 81 cases of PTLD after allo-HSCT were analysed by histopatholgy, immunohistochemistry and in situ hybridisatioin.The cases included 58 males and 23 females with a median age of 26.7 years (range 6-55 years) and the PTLDs developed 1-60 months post-transplant (mean 5.9 months). The histological types indicated 10 cases of non-destructive PTLD, including 4 of plasmacytic hyperplasia, 5 of infectious mononucleosis and 1 of florid follicular hyperplasia. Fifty-six cases were polymorphic PTLD, and 15 were monomorphic PTLD, including thirteen of diffuse large B cell lymphoma, 1 of extranodal nasal type natural killer (NK)/T cell lymphoma and 1 of plasmablastic lymphoma. Foci and sheets of necrosis were observed in 31 cases. The infected ratio of Epstein-Barr virus (EBV) was 91.4%. Some cases were treated by reduction of immunosuppression, antiviral therapy, donor lymphocyte infusion or anti-CD20 monoclonal rituximab. Thirty-three cases died. Compared with that of adult, overall survival of paediatric recipient may be better.The first half year after allo-HSCT is very important for the development of PTLD. Type of PTLD, EBV infection and graft-versus-host disease are risk factors. The prognosis of PTLD is poor, and PTLD after allo-HSCT exhibits some features different from that after solid organ transplantation and some differences existing between adult and paediatric recipients.
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http://dx.doi.org/10.1136/jclinpath-2021-207492DOI Listing
May 2021

High energy density and extremely stable supercapacitors based on carbon aerogels with 100% capacitance retention up to 65,000 cycles.

Proc Natl Acad Sci U S A 2021 May;118(21)

Institute of Materials, Ningbo University of Technology, 315016 Ningbo, People's Republic of China;

In terms of ideal future energy storage systems, besides the always-pursued energy/power characteristics, long-term stability is crucial for their practical application. Here, we report a facile and sustainable strategy for the scalable fabrication of carbon aerogels with three-dimensional interconnected nanofiber networks and rationally designed hierarchical porous structures, which are based on the carbonization of bacterial cellulose assisted by the soft template of Zn-1,3,5-benzenetricarboxylic acid. As binder-free electrodes, they deliver a fundamentally enhanced specific capacitance of 352 F ⋅ g at 1 A ⋅ g in a wide potential window (1.2 V, 6 M KOH) in comparison with those of bacterial cellulose-derived carbons (178 F ⋅ g) and most activated carbons (usually lower than 250 F ⋅ g). The as-assembled supercapacitors exhibit an ultrahigh capacitance of 297 F ⋅ g at 1 A ⋅ g, remarkable energy density (14.83 Wh ⋅ kg at 0.60 kW ⋅ kg), and extremely high stability, with 100% capacitance retention for up to 65,000 cycles at 6 A ⋅ g, representing their superior energy storage performance when compared with that of state-of-the-art supercapacitors of commercial activated carbons and biomass-derived analogs.
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http://dx.doi.org/10.1073/pnas.2105610118DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8166133PMC
May 2021

E3 ligase TRIM25 ubiquitinates RIP3 to inhibit TNF induced cell necrosis.

Cell Death Differ 2021 May 5. Epub 2021 May 5.

State Key Laboratory of Genetic Engineering, Department of Neurology, School of Life Sciences and Huashan Hospital, MOE Engineering Research Center of Gene Technology, Shanghai Engineering Research Center of Industrial Microorganisms, Fudan University, Shanghai, 200438, China.

Receptor interacting protein kinase 3 (RIP3 or RIPK3), the critical executor of cell programmed necrosis, plays essential roles in maintaining immune responses and appropriate tissue homeostasis. Although the E3 ligases CHIP and PELI1 are reported to promote RIP3 degradation, however, how post-translational modification regulates RIP3 activity and stability is poorly understood. Here, we identify the tripartite motif protein TRIM25 as a negative regulator of RIP3-dependent necrosis. TRIM25 directly interacts with RIP3 through its SPRY domain and mediates the K48-linked polyubiquitination of RIP3 on residue K501. The RING domain of TRIM25 facilitates the polyubiquitination chain on RIP3, thereby promoting proteasomal degradation of RIP3. Also, TRIM25 deficiency inhibited the ubiquitination of RIP3, thus promoting TNF-induced cell necrosis. Our current finding reveals the regulating mechanism of polyubiquitination on RIP3, which might be a potential therapeutic target for the intervention of RIP3-dependent necrosis-related diseases.
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http://dx.doi.org/10.1038/s41418-021-00790-3DOI Listing
May 2021

Does Early Postoperative T1 Slope Change Affect Clinical Results of Patients With Single-level ACDF?

Clin Spine Surg 2021 Apr 28. Epub 2021 Apr 28.

Department of Orthopedic Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan Province, China.

Study Design: This was a retrospective study.

Objective: The main question of this study is whether the change of postoperative T1 slope will affect the clinical and imaging recovery of patients with single-level anterior cervical discectomy and fusion (ACDF).

Summary Of Background Data: The T1 slope after ACDF is different from that preoperatively, however, the clinical significance of this change has not been reported.

Materials And Methods: A retrospective analysis was conducted on 87 patients with single-level ACDF. Visual Analogue Scale was used to compare neck and upper limb pain before and after surgery. Neurological improvement was assessed by the Japanese Orthopaedic Association Scores and Neck Disability Index. Preoperative and postoperative T1 slope, occipital-C2 angle, C2-C7 overall curvature and functional spinal unit curvature were measured and analyzed by lateral cervical spine x-ray.

Results: All patients were followed up for 23.98±12.17 months. The Japanese Orthopaedic Association, Visual Analogue Scale, and Neck Disability Index scores as well as the overall curvature and change of C2-C7 and functional spinal unit were significantly improved in the last postoperative follow-up. At 12 months after surgery and the last follow-up, patients with increased T1 slope had more severe neck pain symptoms than those with decreased T1 slope (P<0.05). The overall curvature and change of C2-C7 in patients with increased T1 slope were better than those with decreased T1 slope (P<0.05).

Conclusion: For patients with increased postoperative T1 slope after single-level ACDF, the degree of postoperative neck pain was more severe, suggesting that some clinical intervention is needed.
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http://dx.doi.org/10.1097/BSD.0000000000001145DOI Listing
April 2021

Polypeptide N-acetylgalactosaminyltransferase 18 Retains in Endoplasmic Reticulum Depending on its Luminal Regions Interacting with ER resident UGGT1, PLOD3 and LPCAT1.

Glycobiology 2021 Apr 28. Epub 2021 Apr 28.

Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China.

Mucin-type O-glycosylation is initiated by the UDP-GalNAc polypeptide:N-acetylgalactosaminyltransferase (ppGalNAc-T) family of enzymes, which consists of 20 members in humans. Among them, unlike other ppGalNAc-Ts located in Golgi apparatus, ppGalNAc-T18 distributes primarily in the endoplasmic reticulum (ER) and non-catalytically regulates ER homeostasis and O-glycosylation. Here, we report the mechanism for ppGalNAc-T18 ER localization and the function of each structural domain of ppGalNAc-T18. By using ppGalNAc-T18 truncation mutants, we revealed that the luminal stem region and catalytic domain of ppGalNAc-T18 are essential for ER localization, whereas the lectin domain and N-glycosylation of ppGalNAc-T18 are not required. In the absence of the luminal region (i.e., stem region, catalytic and lectin domains), the conserved Golgi retention motif RKTK within the cytoplasmic tail combined with the transmembrane domain ensure ER export and Golgi retention, as observed for other Golgi resident ppGalNAc-Ts. Results from co-immunoprecipitation assays showed that the luminal region interacts with ER resident proteins UGGT1, PLOD3 and LPCAT1. Furthermore, flow cytometry analysis showed that the entire luminal region is required for the non-catalytic O-GalNAc glycosylation activity of ppGalNAc-T18. The findings reveal a novel subcellular localization mechanism of ppGalNAc-Ts and provide a foundation to further characterize the function of ppGalNAc-T18 in the ER.
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http://dx.doi.org/10.1093/glycob/cwab031DOI Listing
April 2021

[Effect of modified lateral mass screws implantation strategy on axial symptoms in cervical expansive open-door laminoplasty].

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2021 Apr;35(4):445-452

Department of Orthopedics, West China Hospital, Sichuan University, Chengdu Sichuan, 610041, P.R.China.

Objective: To investigate the effect of modified lateral mass screws implantation strategy on axial symptoms in cervical expansive open-door laminoplasty.

Methods: A clinical data of 166 patients, who underwent cervical expansive open-door laminoplasty between August 2011 and July 2016 and met the selection criteria, was retrospective analyzed. Among them, 81 patients were admitted before August 2014 using the traditional mini-plate placement and lateral mass screws implantation strategy (control group), and 85 patients were admitted after August 2014 using modified lateral mass screws implantation strategy (modified group). There was no significant difference in the gender composition, age, clinical diagnosis, disease duration, diseased segment, and preoperative Japanese Orthopaedic Association (JOA) score, pain visual analogue scale (VAS) score, Neck Disability Index (NDI), cervical curvature and range of motion, spinal canal diameter and cross-sectional areas, and Pavlov's value between the two groups ( >0.05). The operation time, intraoperative blood loss, the number of facet joints penetrated by lateral mass screws, effectiveness evaluation indexes (JOA score and improvement rate, VAS score, NDI), imaging evaluation indexes (cervical curvature and range of motion, spinal canal diameter and cross-sectional areas, Pavlov's value, and lamina open angle), and complications were recorded and compared between the two groups.

Results: The modified group had shorter operation time and lower intraoperative blood loss than the control group ( <0.05). There were 121 (29.9%, 121/405) and 10 (2.4%, 10/417) facet joints penetrated by lateral mass screws in control and modified groups, respectively; and the difference in incidence was significant ( =115.797, =0.000). Eighteen patients in control group had 3 or more facet joints penetrated while no patients in modified group suffered 3 or more facet joint penetrated. The difference between the two groups was significant ( =0.000). All patients were followed up, the follow-up time was (28.7±4.9) months in modified group and (42.4±10.7) months in control group, showing significant difference ( =10.718, =0.000). The JOA score, VAS score, and NDI at last follow-up of the two groups were significantly improved compared with preoperative ( <0.05); there was no significant difference in JOA score and improvement rate and VAS score between the two groups ( >0.05), but the NDI was significantly lower in modified group than in control group ( <0.05). There were significant differences in cervical curvature and range of motion, spinal canal diameter, Pavlov's value, and cross-sectional areas at last follow-up when compared with those before operation in both groups ( <0.05). There was no significant difference in the above indicators and lamina open angle between the two groups ( >0.05). The modified group has a relative lower axial symptom rate (23/85, 27.1%) than the control group (27/81, 33.3%), but the difference was not significant ( =-1.446, =0.148). There was no significant differences between the two groups in the incidences of C nerve root palsy, cerebrospinal fluid leakage, wound infection, and lung or urinary tract infection ( >0.05).

Conclusion: In the cervical expansive open-door laminoplasty, the modified lateral mass screws implantation strategy can effectively reduce the risk of lateral mass screw penetrated to the cervical facet joints, and thus has a positive significance in avoiding the axial symptoms caused by facet joint destruction.
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http://dx.doi.org/10.7507/1002-1892.202010024DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8171624PMC
April 2021

Characterization of a Recombinant D-Allulose 3-epimerase from Thermoclostridium caenicola with Potential Application in D-Allulose Production.

Mol Biotechnol 2021 Jun 29;63(6):534-543. Epub 2021 Mar 29.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, Jiangsu, People's Republic of China.

In recent years, with the increasing public health awareness, low-calorie rare sugars have received more attention on a global scale. D-Allulose, the C-3 epimer of D-fructose, is a representative rare sugar. It displays high sweetness and excellent physiological functions, but only provides a caloric value of 0.4 kcal/g. D-Allulose 3-epimerase (DAEase) is indispensable in D-allulose production. In this study, a putative DAEase from Thermoclostridium caenicola was identified and characterized. The novel T. caenicola DAEase displayed maximum activity at pH 7.5 and 65 °C in the presence of 1 mM Co. The half-life (t) at 50 °C was 13.6 h, and the melting temperature (T) was 62.4 °C. It was strictly metal-dependent, and the addition of Co remarkably enhanced its thermostability, with a 5.4-fold increase in t value at 55 °C and 4.8 °C increase in T. Furthermore, DAEase displayed high relative activity (89.0%) at a weakly acidic pH 6.5 and produced 139.8 g/L D-allulose from 500 g/L D-fructose, achieving a conversion ratio of 28.0%. These findings suggest that T. caenicola DAEase is a promising biocatalyst for the production of D-allulose.
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http://dx.doi.org/10.1007/s12033-021-00320-zDOI Listing
June 2021

Ultrahigh-Aspect-Ratio Boron Nitride Nanosheets Leading to Superhigh In-Plane Thermal Conductivity of Foldable Heat Spreader.

ACS Nano 2021 Apr 18;15(4):6489-6498. Epub 2021 Mar 18.

Key Laboratory of Marine Materials and Related Technologies, Zhejiang Key Laboratory of Marine Materials and Protective Technologies, Ningbo Institute of Materials Technology and Engineering (NIMTE), Chinese Academy of Sciences, Ningbo, 315201, P. R. China.

The rapid development of integrated circuits and electronic devices creates a strong demand for highly thermally conductive yet electrically insulating composites to efficiently solve "hot spot" problems during device operation. On the basis of these considerations, hexagonal boron nitride nanosheets (BNNS) have been regarded as promising fillers to fabricate polymer matrix composites. However, so far an efficient approach to prepare ultrahigh-aspect-ratio BNNS with large lateral size while maintaining an atomically thin nature is still lacking, seriously restricting further improvement of the thermal conductivity for BNNS/polymer composites. Here, a rapid and high-yield method based on a microfluidization technique is developed to obtain exfoliated BNNS with a record high aspect ratio of ≈1500 and a low degree of defects. A foldable and electrically insulating film made of such a BNNS and poly(vinyl alcohol) (PVA) matrix through filtration exhibits an in-plane thermal conductivity of 67.6 W m K at a BNNS loading of 83 wt %, leading to a record high value of thermal conductivity enhancement (≈35 500). The composite film then acts as a heat spreader for heat dissipation of high-power LED modules and shows superior cooling efficiency compared to commercial flexible copper clad laminate. Our findings provide a practical route to produce electrically insulating polymer composites with high thermal conductivity for thermal management applications in modern electronic devices.
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http://dx.doi.org/10.1021/acsnano.0c09229DOI Listing
April 2021

Overexpressed NEDD8 as a potential therapeutic target in esophageal squamous cell carcinoma.

Cancer Biol Med 2021 Mar 18. Epub 2021 Mar 18.

Department of Laboratory Medicine, Huadong Hospital Affiliated to Fudan University, Shanghai 200040, China.

Objective: The hyperactivated neddylation pathway plays an important role in tumorigenesis and is emerging as a promising anticancer target. We aimed to study whether NEDD8 (neural precursor cell expressed, developmentally down-regulated 8) might serve as a therapeutic target in esophageal squamous cell carcinoma (ESCC).

Methods: The clinical relevance of NEDD8 expression was evaluated by using The Cancer Genome Atlas (TCGA) database and tissue arrays. NEDD8-knockdown ESCC cells generated with the CRISPR/Cas9 system were used to explore the anticancer effects and mechanisms. Quantitative proteomic analysis was used to examine the variations in NEDD8 knockdown-induced biological pathways. The cell cycle and apoptosis were assessed with fluorescence activated cell sorting. A subcutaneous-transplantation mouse tumor model was established to investigate the anticancer potential of NEDD8 silencing .

Results: NEDD8 was upregulated at both the mRNA and protein expression levels in ESCC, and NEDD8 overexpression was associated with poorer overall patient survival (mRNA level: = 0.028, protein level: = 0.026, log-rank test). Downregulation of NEDD8 significantly suppressed tumor growth both and . Quantitative proteomic analysis revealed that downregulation of NEDD8 induced cell cycle arrest, DNA damage, and apoptosis in ESCC cells. Mechanistic studies demonstrated that NEDD8 knockdown led to the accumulation of cullin-RING E3 ubiquitin ligases (CRLs) substrates through inactivation of CRLs, thus suppressing the malignant phenotype by inducing cell cycle arrest and apoptosis in ESCC. Rescue experiments demonstrated that the induction of apoptosis after NEDD8 silencing was attenuated by DR5 knockdown.

Conclusions: Our study elucidated the anti-ESCC effects and underlying mechanisms of NEDD8 knockdown, and validated NEDD8 as a potential target for ESCC therapy.
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http://dx.doi.org/10.20892/j.issn.2095-3941.2020.0484DOI Listing
March 2021

Intramedullary nails in combination with reconstruction plate in the treatment of unstable intertrochanteric femoral fractures with lateral wall damage.

Int Orthop 2021 Mar 17. Epub 2021 Mar 17.

Department of Trauma Orthopedic, The Naval Medical University, Shanghai, 200433, China.

Background: The unstable intertrochanteric femur fracture remains a challenge for surgeons. However, few studies have compared the clinical effectiveness of intramedullary nail in combination with a reconstruction plate and intramedullary nail alone in the treatment of patients with unstable intertrochanteric femoral fractures with lateral wall damage.

Methods: This study retrospectively analyzed 16 patients with 31 A3 intertrochanteric fractures treated with the intramedullary nail in combination with reconstruction plate (the study group) and 19 patients with 31 A3 intertrochanteric fractures treated with intramedullary nail alone (the control group) between January 2012 and January 2018. The operation time, intra-operative blood loss, time of fracture healing, and complication rates of post-operative fixation failure were assessed between the two groups. At the follow-up of post-operative six and 12 months, Harris hip score (HHS) and the Parker-Palmer mobility score (PPMS) were used to evaluate the functional states and mobility levels.

Results: The distribution of all basic characteristics was similar between the two groups (P ˃ 0.05). The study group had longer operation time and more intra-operative blood loss in comparison with the control group (P < 0.001), while the study group had shorter fracture healing time (P = 0.03) and lower fixation failure rate as compared with the control group. Regarding the functional outcome, the study group had higher HHSs and PPMS than the control group (P = 0.003).

Conclusions: Although intramedullary nails in combination with reconstruction plates had longer operation time and more intra-operative blood loss, it might be superior to intramedullary nail alone in terms of fracture healing time, fixation failure complication rate, and post-operative functional recovery.
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http://dx.doi.org/10.1007/s00264-021-05004-6DOI Listing
March 2021

Co-Zeolitic Imidazolate [email protected] Aerogels from Sugarcane Bagasse for Activating Peroxymonosulfate to Degrade P-Nitrophenol.

Polymers (Basel) 2021 Feb 27;13(5). Epub 2021 Feb 27.

Guangxi Institute Fullerene Technology (GIFT), Key Laboratory of New Processing Technology for Nonferrous Metals and Materials, Ministry of Education, School of Resources, Environment and Materials, Guangxi University, Nanning 530004, China.

An efficient, green and reusable catalyst for organic pollutant wastewater treatment has been a subject of intense research in recent decades due to the limitation of current technologies. Cellulose based aerogel composites are considered to be an especially promising candidate for next-generation catalytic material. This project was conducted in order to evaluate the behavior and ability of green and reusable sugarcane bagasse aerogels to remove P-Nitrophesnol from waste-water aqueous. Co-Zeolitic imidazolate [email protected] sugarcane bagasse aerogels composite catalysts were successfully prepared via simple in situ synthesis. The structure of hybrid aerogels and their efficient catalyst in peroxymonosulfate (PMS) activation for the degradation of p-nitrophenol (PNP) was investigated. As a result, the hybrid aerogels/PMS system removed 98.5% of PNP (10mg/L) within 60~70 min, while the traditional water treatment technology could not achieve this. In addition, through a free radical capture experiment and electron paramagnetic resonance (EPR), the degradation mechanism of PNP was investigated. Further research found that the hybrid aerogels can effectively activate PMS to produce sulfate (SO) and hydroxyl (OH ). Both of them contributed to the degradation of PNP, and SO plays a crucial role in the degradative process. The most important feature of hybrid aerogels can be easily separated from the solution. The obtained results showed that the outer coating structure of cellulose can stabilize Co-ZIF and reduce the dissolution of cobalt ions under complex reaction conditions. Moreover, the prepared hybrid aerogels exhibit excellent reusability and are environmentally friendly with efficient catalytic efficiency. This work provides a new strategy for bagasse applications and material reusability.
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http://dx.doi.org/10.3390/polym13050739DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7957538PMC
February 2021

A Novel Anterior Cervical X-Shape-Corpectomy and Fusion for Cervical Spinal Stenosis at C4-C6 Level: A Technical Note.

World Neurosurg 2021 May 2;149:181-189. Epub 2021 Mar 2.

Department of Anesthesia and Operation Center/ West China School of Nursing, West China Hospital of Sichuan University, Chengdu, China.

Objective: Anterior cervical corpectomy and fusion (ACCF) is employed in patients with localized cervical spinal stenosis (CSS). However, there are some disadvantages such as subsidence of the titanium mesh cage, slow fusion rates, breakage of the plate and screws, and donor-site complications. For patients with small posterior osteophytes, ossified or hypertrophy of the posterior longitudinal ligaments or ligamentum flavum, the range of decompression from the classic anterior cervical discectomy and fusion (ACDF) cannot meet the clinical requirements. However, employing ACCF is controversial. Therefore, it is necessary to seek a novel, safe and effective surgery that can combine the strengths of ACDF and ACCF. Our objective was to describe a novel anterior approach cervical surgery and investigate its clinical outcomes on segmental CSS at the C4-C6 levels 6 months postoperatively.

Methods: A novel anterior cervical X-shape-corpectomy and fusion (ACXF) was performed to correct the CSS.

Results: The patient's neurologic function and myodynamia of the extremities were improved significantly 3 and 6 months after surgery with good bony fusion. Neck pain also was relieved. Immediately postoperative and after 6-month images indicated no significant spinal stenosis. The patient's cervical curvature was improved after surgery without significant implant subsidence or loss of adjacent intervertebral height. There were no postoperative complications.

Conclusions: ACXF may be a safe and effective procedure for segmental CSS and an alternative for ACCF, as it has a wide operative field of view, sufficient decompression range, excellent transverse vertebral bony fusion, less internal fixation-related complications, and graft subsidence and no donor-site complications.
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http://dx.doi.org/10.1016/j.wneu.2021.02.098DOI Listing
May 2021

Metabolomic alterations associated with copper stress in .

Future Microbiol 2021 Mar 26;16:305-316. Epub 2021 Feb 26.

Department of Clinical Laboratory, State Key Laboratory of Complex Severe & Rare Diseases, Peking Union Medical College Hospital, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing, China.

Copper stress is an effective host strategy in resisting the opportunistic pathogenic fungus . We studied metabolic changes in under copper stress. Wild-type and metallothionein-null were treated with copper on agar containing glucose, glycerol or ethanol as the carbon source and their metabolites were analyzed by untarget metabolomics strategy using gas chromatography coupled with time-of-flight mass spectrometry. The metabolic profile of varied in the presence and absence of copper. Pathway enrichment analysis showed that the differentially abundant metabolites were related to amino acid and carbohydrate metabolism. grown on glycerol or ethanol resisted copper toxicity better than grown on glucose. Copper stress alters the metabolic profile of .
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http://dx.doi.org/10.2217/fmb-2020-0290DOI Listing
March 2021

Regulative Electronic States around Ruthenium/Ruthenium Disulphide Heterointerfaces for Efficient Water Splitting in Acidic Media.

Angew Chem Int Ed Engl 2021 May 30;60(22):12328-12334. Epub 2021 Mar 30.

State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan, 430070, P. R. China.

Theoretical calculations unveil the charge redistribution over abundant interfaces and the enhanced electronic states of Ru/RuS heterostructure. The resulting surface electron-deficient Ru sites display optimized adsorption behavior toward diverse reaction intermediates, thereby reducing the thermodynamic energy barriers. Experimentally, for the first time the laminar Ru/RuS heterostructure is rationally engineered by virtue of the synchronous reduction and sulfurization under eutectic salt system. Impressively, it exhibits extremely high catalytic activity for both OER (201 mV @ 10 mA cm ) and HER (45 mV @ 10 mA cm ) in acidic media due to favorable kinetics and excellent specific activity, consequently leading to a terrific performance in acidic overall water splitting devices (1.501 V @ 10 mA cm ). The in-depth insight into the internal activity origin of interfacial effect could offer precise guidance for the rational establishment of hybrid interfaces.
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http://dx.doi.org/10.1002/anie.202101539DOI Listing
May 2021

A novel DNA and protein combination COVID-19 vaccine formulation provides full protection against SARS-CoV-2 in rhesus macaques.

Emerg Microbes Infect 2021 Dec;10(1):342-355

Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, People's Republic of China.

The current study aims to develop a safe and highly immunogenic COVID-19 vaccine. The novel combination of a DNA vaccine encoding the full-length Spike (S) protein of SARS-CoV-2 and a recombinant S1 protein vaccine induced high level neutralizing antibody and T cell immune responses in both small and large animal models. More significantly, the co-delivery of DNA and protein components at the same time elicited full protection against intratracheal challenge of SARS-CoV-2 viruses in immunized rhesus macaques. As both DNA and protein vaccines have been proven safe in previous human studies, and DNA vaccines are capable of eliciting germinal center B cell development, which is critical for high-affinity memory B cell responses, the DNA and protein co-delivery vaccine approach has great potential to serve as a safe and effective approach to develop COVID-19 vaccines that provide long-term protection.
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http://dx.doi.org/10.1080/22221751.2021.1887767DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7928010PMC
December 2021

LncRNA H19 regulates macrophage polarization and promotes Freund's complete adjuvant-induced arthritis by upregulating KDM6A.

Int Immunopharmacol 2021 Apr 1;93:107402. Epub 2021 Feb 1.

Department of Immunology, Mudanjiang Medical University, Mudanjiang, Heilongjiang 157011, China. Electronic address:

Aberrant expression of long non-coding RNA (lncRNA) H19 is tightly linked to multiple steps of tumorigenesis via the modulation of cell proliferation and apoptosis; however, the pathological significance and regulatory mechanisms of lncRNA H19 in macrophages remain obscure. To investigate whether lncRNA H19 modulates macrophage activation in rheumatoid arthritis (RA), lncRNA H19 levels in PMA-induced PBMC from patients with RA and healthy volunteers were assessed. In addition, the distribution of macrophage subsets, macrophage phenotypic characteristics, and pro-inflammatory gene expression were examined in lncRNA H19 smart silencer- or pcDNA 3.1- H19-transfected macrophages and AAV8-mediated H19 overexpression in a Freund' s complete adjuvant-induced arthritis mouse model. The level of lncRNA H19 was higher in RA patients than in healthy volunteers. Silencing of lncRNA H19 altered lipopolysaccharide plus interferon-induced M1 macrophage polarization and decreased IL-6, CD80, CCL8, and CXCL10 expression in macrophages of RA patients. LncRNA H19 overexpression markedly induced IL-6, CD80, HLA-DR, KDM6A, STAT1, IRF5, CCL8, CXCL9, CXCL10, and CXCL11 expression in macrophages and promoted macrophage migration. AAV8-mediated H19 overexpression aggravated arthritis in mice by promoting M1 macrophage polarization along with iNOS, IL-6, CCL8, CXCL9, CXCL10, CXCL11, MMP3, MMP13 and COX-2 expression in mononuclear cells isolated from the swollen ankle. GSK-J4, an inhibitor of KDM6A, suppressed the activity of lncRNA H19 in macrophages and ameliorated lncRNA H19-aggravated arthritis. In summary, the current study demonstrated that lncRNA H19 is upregulated in RA patients and arthritic mice. LncRNA H19 promotes M1 macrophage polarization and aggravates arthritis by upregulating KDM6A expression.
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http://dx.doi.org/10.1016/j.intimp.2021.107402DOI Listing
April 2021

Author Correction: ALK phosphorylates SMAD4 on tyrosine to disable TGF-β tumour suppressor functions.

Nat Cell Biol 2021 Mar;23(3):293

MOE Key Laboratory of Biosystems Homeostasis & Protection and Innovation Center for Cell Signaling Network, Life Sciences Institute, Zhejiang University, Hangzhou, China.

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http://dx.doi.org/10.1038/s41556-021-00638-5DOI Listing
March 2021

Routinely placing drainage tube in patients with anterior cervical surgery: is it really necessary?

Chin Med J (Engl) 2021 Feb 1;134(5):521-523. Epub 2021 Feb 1.

Department of Anesthesia and Operation Center/West China School of Nursing, West China Hospital of Sichuan University, Chengdu, Sichuan 610000, China.

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http://dx.doi.org/10.1097/CM9.0000000000001253DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7929629PMC
February 2021

Effect of Facet Tropism on Postoperative Cervical Range of Motion After Single-Level Cervical Disc Arthroplasty.

Global Spine J 2021 Jan 29:2192568220986144. Epub 2021 Jan 29.

Department of Orthopedics, West China Hospital, 34753Sichuan University, Chengdu, Sichuan Province, China.

Study Design: Retrospective analysis.

Objectives: Cervical disc arthroplasty (CDA) was designed to replace the degenerated disc with the prosthesis to preserve cervical motion. The commonly used artificial discs are designed symmetric, whereas the facet joints were reported to be asymmetric in many people. This study aimed to evaluate the effect of facet tropism on the cervical range of motion (ROM) after single-level CDA using Prestige LP.

Methods: A total of 90 patients who underwent single-level CDA using Prestige LP from 2012 to 2017 were retrospectively reviewed. Radiographs were taken at each time point to measure the C2-C7 ROM and the ROM at the surgical segment. The pre-operation CT scans were utilized to reconstruct and calculate the angular direction of facet joints with respect to transverse, coronal, and sagittal reference planes. Facet tropism above 7° was defined as facet joint asymmetry.

Results: No significant difference was found in flexion-extension C2-C7 ROM or ROM at the surgical segment between patients with symmetric and asymmetric fact joints regarding the sagittal plane. Patients with coronal asymmetric facet joints had lower flexion-extension ROM at the surgical level. Patients with transverse asymmetric facet joints had both lower flexion-extension C2-C7 ROM and ROM at the surgical level. After CDA surgery, patients obtained good clinical outcomes including increased Japanese Orthopedic Association (JOA) and decreased Neck Disability Index (NDI) as well as Visual Analogue Scale (VAS).

Conclusion: The coronal and transverse tropism seemed to be correlated with decreased flexion-extension ROM after CDA using Prestige LP.
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http://dx.doi.org/10.1177/2192568220986144DOI Listing
January 2021
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