Publications by authors named "Dian Chen"

65 Publications

Epithelial microRNA-206 targets CD39/extracellular ATP to upregulate airway IL-25 and TSLP in type 2-high asthma.

JCI Insight 2021 May 4. Epub 2021 May 4.

Department of Internal Medicine, Tongji Hospital, Wuhan, China.

The epithelial cell-derived cytokines IL-25, IL-33 and TSLP initiate type 2 inflammation in allergic diseases including asthma. However, the signaling pathway regulating these cytokines expression remains elusive. Since microRNAs are pivotal regulators of gene expression, we profiled microRNA expression in bronchial epithelial brushings from type 2-low and type 2-high asthma patients. MiR-206 was the most highly expressed epithelial microRNA in type 2-high asthma relative to type 2-low asthma but was downregulated in both subsets compared with healthy controls. CD39, an ectonucleotidase degrading ATP, was a target of miR-206 and upregulated in asthma. Allergen-induced acute extracellular ATP accumulation led to miR-206 downregulation and CD39 upregulation in human bronchial epithelial cells, forming a feedback loop to eliminate excessive ATP. Airway ATP levels were markedly elevated and strongly correlated with IL-25 and TSLP expression in asthma patients. Intriguingly, airway miR-206 antagonism increased Cd39 expression, reduced ATP accumulation, suppressed Il-25, Il-33, Tslp expression and group 2 innate lymphoid cell expansion, and alleviated type 2 inflammation in a mouse model of allergic airway inflammation. In contrast, airway miR-206 overexpression had opposite effects. Overall, epithelial miR-206 upregulates airway IL-25, TSLP expression by targeting CD39-extracellular ATP axis, which represents a novel therapeutic target in type 2-high asthma.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1172/jci.insight.148103DOI Listing
May 2021

Malignant transformation arising from mature ovarian cystic teratoma: A case series.

Medicine (Baltimore) 2021 Apr;100(13):e24726

Department of Oncology.

Abstract: Malignant transformation arising in mature cystic teratoma (MT-MCT) is a rare neoplasm of the ovary. Herein, we aimed to evaluate the clinicopathological features and treatment outcome of the Han Chinese women with MT-MCT.In this retrospective study, the clinical data of patients who had been surgically treated from January 2000 to November 2019 and in whom the diagnosis of MCT was confirmed based on the pathology were included. Fourteen patients with MT-MCT from a total of 569 cases (2.46% incidence) of MCT were reviewed.The mean age of patients with MT-MCT was 51.3 (range, 31-71) years, while the mean age of patients with MCT was 45.3 (range, 17-62) years. Upon gross examination, the mean size of MT-MCT was 14.0 (range, 11-25) cm, whereas the mean size of MCT was 7.5 (range, 4-10) cm. Primary surgical staging was performed in all cases. Complete cytoreduction and suboptimal surgical resection were performed in 12 (85.7%) and 2 (14.3%) cases, respectively. Thirteen patients with malignant transformation of squamous cell carcinoma (SCC) whose Federation International of Gynecology and Obstetrics stage was >1 received chemotherapy, comprising carboplatin and paclitaxel. Response to the chemotherapy regimen was complete in 12 patients; 1/12 patients died within the median follow-up period of 16.5 months. The 5-year overall survival rate and disease-free survival rates were 31.2% and 31.6%, respectively.From the data generated, we conclude that the rate of MT-MCT increases with age. The MT-MCT was much higher in women of postmenopausal age than in younger women. We described our experience of successfully treating patients with malignant transformation of SCC with primary surgical staging and adjuvant chemotherapy (cisplatin, paclitaxel, bleomycin, and etoposide) that might improve survival in patients with advanced-stage disease.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/MD.0000000000024726DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8021333PMC
April 2021

Coinducible Catabolism of 1-Naphthol via Synergistic Regulation of the Initial Hydroxylase Genes in sp. Strain B2.

Appl Environ Microbiol 2021 May 11;87(11). Epub 2021 May 11.

Department of Microbiology, Key Laboratory of Microbiology for Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, China

1-Naphthol, a widely used raw material for organic synthesis, is also a well-known organic pollutant. Due to its high toxicity, 1-naphthol is rarely used by microorganisms as the sole carbon source for growth. In this study, catabolism of 1-naphthol by sp. strain B2 was found to be greatly enhanced by additional supplementation with primary carbon sources (e.g., glucose, maltose, and sucrose), and 1-naphthol was even used as the carbon source for growth when strain B2 cells had been preinduced by both 1-naphthol and glucose. A distinct two-component flavin-dependent monooxygenase, NdcA1A2, was found to be responsible for the initial hydroxylation of 1-naphthol to 1,2-dihydroxynaphthalene, a more toxic compound. Transcriptional levels of genes were significantly upregulated when strain B2 cells were cultured with both 1-naphthol and glucose compared to cells cultured with only 1-naphthol or glucose. Two transcriptional regulators, the activator NdcS and the inhibitor NdcR, were found to play key roles in the synergistic regulation of the transcription of the 1-naphthol initial catabolism genes Cometabolism is a widely observed phenomenon, especially in the field of microbial catabolism of highly toxic xenobiotics. However, the mechanisms of cometabolism are ambiguous, and the roles of the obligately coexisting growth substrates remain largely unknown. In this study, we revealed that the roles of the coexisting primary carbon sources (e.g., glucose) in the enhanced catabolism of the toxic compound 1-naphthol in sp. strain B2 were not solely because they were used as growth substrates to support cell growth but, more importantly, because they acted as coinducers to interact with two transcriptional regulators, the activator NdcS and the inhibitor NdcR, to synergistically regulate the transcription of the 1-naphthol initial catabolism genes Our findings provide new insights into the cometabolic mechanism of highly toxic compounds in microorganisms.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1128/AEM.00170-21DOI Listing
May 2021

A Comparison of Efficacy and Safety of Fractional Carbon Dioxide Laser and Fractional Er:YAG Laser for the Treatment of Xanthelasma Palpebrarum: A Two-Center Randomized Split-Face Controlled Trial.

Photobiomodul Photomed Laser Surg 2021 Feb 15;39(2):131-136. Epub 2021 Jan 15.

Department of Dermatology, School of Clinical Medicine, Beijing Tsinghua Changgung Hospital, Tsinghua University, Beijing, China.

Xanthelasma palpebrarum (XP) is a form of cutaneous xanthoma that presents as collections of yellowish papules or plaques around the eyelids or canthus, affecting patients cosmetically. This study aimed to compare the efficacy and safety of fractional carbon dioxide (CO) laser to that of fractional Er:YAG laser for the treatment of XP. Two centers recruited patients diagnosed with XP of bilaterally symmetrical lesions. The lesion on one side was randomly assigned to be treated with fractional CO laser while the lesion on the other side was treated with fractional Er:YAG laser. All subjects received up to five treatments, with a 4-week interval between each treatment. Thirty-nine patients completed the study and a total of 82 lesions were available for final assessment. The percentage of "Excellent Improvement" on third and fourth visit was 60.98% versus 39.02% and 90.24% versus 63.41%, respectively,  < 0.05. In a follow-up for 12 to 25 months, the number of lesions recurred on the side treated with fractional CO laser and fractional Er:YAG laser are 9 (22%) and 10 (24%), respectively. In this study, fractional CO laser therapy appears superior since a fewer treatments are required for patients to show significant clinical improvement.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1089/photob.2020.4874DOI Listing
February 2021

Epidermoid cyst removal with CO laser fenestration: A retrospective cohort study.

J Cosmet Dermatol 2020 Oct 20. Epub 2020 Oct 20.

Department of Dermatology, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua University, Beijing, China.

Background: Epidermoid cyst (EC) is a common and benign tumor, which can occur anywhere on the skin. Surgical excision is usually considered as the first-line treatment. However, linear scars arising after excision for EC remain a cosmetic problem.

Objective: To evaluate the efficacy and safety of EC removal assisted with CO laser fenestration.

Methods: All patients diagnosed with EC and treated with CO laser fenestration, content extrusion, and removal of the cyst wall between January 1, 2016, and December 31, 2018, were included in this study. After a follow-up period ranging from 6 to 27 months, scarring, recurrence, complications, and satisfaction were assessed and analyzed.

Results: Forty-three of the 47 patients have been cured by a single operation. The recurrence rate was 8.5%, which was not significantly correlated with tumor sizes or locations. 46.8% of the patients had no obvious scar after treatment. No infections or complications were observed in any of these cases. 89.4% of the patients were satisfied with the effectiveness of the treatment, while 95.7% and 87.2% were satisfied with the comfort and the cosmetic results, respectively.

Conclusions: CO laser fenestration-assisted removal procedure is effective for the treatment of ECs with good aesthetic outcome.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/jocd.13766DOI Listing
October 2020

Predictors of fatality including radiographic findings in adults with COVID-19.

Respir Res 2020 Jun 11;21(1):146. Epub 2020 Jun 11.

Division of Respiratory and Critical Care Medicine, Department of Internal Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.

Background: Older age and elevated d-dimer are reported risk factors for coronavirus disease 2019 (COVID-19). However, whether early radiographic change is a predictor of fatality remains unknown.

Methods: We retrospectively reviewed records of all laboratory-confirmed patients admitted to a quarantine unit at Tongji Hospital, a large regional hospital in Wuhan, China, between January 31 and March 5, 2020. Confirmed cases were defined by positive RT-PCR detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in throat-swab specimens. Chest CT images were reviewed independently by two radiologists. The Tongji Hospital ethics committee approved this study.

Results: A total of 102 patients were confirmed to have SARS-CoV-2 infection. As of March 25, 85 confirmed patients were discharged, 15 died, and 2 remained hospitalized. When compared with survivors, non-survivors were older (median age, 69 [interquartile range, 58-77] vs. 55 [44-66], p = 0.003), and more likely to have decreased lymphocyte count (0.5 vs. 0.9 ×  10/L, p = 0.006), elevated lactate dehydrogenase (LDH) (569.0 vs. 272.0 U/L, p < 0.001), elevated d-dimer (> 1 μg/mL, 86% vs. 37%, p = 0.002) on admission. Older age and elevated LDH were independent risk factors for fatality in a multivariate regression model included the above variables. In a subset of patients with CT images within the first week, higher total severity score, and more involved lung lobes (5 involved lobes) in CT images within the first week were significantly associated with fatality. Moreover, in this subset of patients, higher total severity score was the only independent risk factor in a multivariate analysis incorporating the above mentioned variables.

Conclusions: Older age, elevated LDH on admission, and higher severity score of CT images within the first week are potential predictors of fatality in adults with COVID-19. These predictors may help clinicians identify patients with a poor prognosis at an early stage.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12931-020-01411-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7289230PMC
June 2020

A new method for the preparation of three-layer vascular stents: a preliminary study on the preparation of biomimetic three-layer vascular stents using a three-stage electrospun membrane.

Biomed Mater 2020 07 27;15(5):055010. Epub 2020 Jul 27.

Department of Cardiothoracic Surgery, Shanghai Children's Medical Center, Shanghai Jiaotong University School of Medicine, Shanghai 200127, People's Republic of China. These authors contributed equally to this work.

There is an urgent need to design a tissue-engineered vascular graft that exhibits good biocompatibility and sufficient mechanical strength to repair and facilitate regeneration of defective vascular tissue. It is generally accepted that multi-layer stents can be used to simulate the structure and function of natural blood vessels. Here, we developed a new three-layer tubular graft that is rolled from a single Poly(L-lactide-co-caprolactone) electrospun membrane. We used a new electrospinning technique to place three different structures on a single electrospun membrane such that the stent is comprised of three different layers. The inner layer is dense and suitable for endothelial cell growth, the middle layer is a parallel loose structure suitable for smooth muscle cell growth, and the outer layer is a parallel structure with sparse alternating texture suitable for both smooth muscle cell growth and structural support. The vascular stent has good tensile strength. At the same time, endothelial cells and smooth muscle cells readily proliferate on the material in vitro. In particular, smooth muscle cells grow in parallel on the middle and outer materials. In vivo, all layers of the vascular graft were infiltrated by cells within one week of subcutaneous implantation, indicative of favorable biocompatibility. After a week of subcutaneous implantation, the vascular stent was orthotopically transplanted into the abdominal aorta of Sprague Dawley rats. After ten weeks of transplantation, ultrasound imaging of the abdomen showed vascular patency. The vascular stent was endothelialized, smooth muscle cells readily proliferated, and a large amount of elastic fibers were formed. Therefore, our specially designed tri-layer vascular graft may be of significant benefit in vascular reconstruction.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1088/1748-605X/ab920aDOI Listing
July 2020

Midterm Outcomes of Surgical Repair of Partial Anomalous Pulmonary Venous Connection to SVC.

Thorac Cardiovasc Surg 2020 01 15;68(1):24-29. Epub 2019 Nov 15.

Department of Cardio-Thoracic Surgery, Shanghai Children's Medical Center Affiliated to Shanghai Jiao Tong, University School of Medicine, Shanghai, China.

Objectives: We explore midterm results after surgical treatment of partial anomalous pulmonary venous connection (PAPVC) to superior vena cava (SVC) in our institution.

Methods: From 2008 to 2017, 78 patients underwent surgical repair for PAPVC to SVC. Patients were divided into three groups based on surgical techniques: Single-patch repair ( = 20, group A), double-patch repair ( = 31, group B), and Warden repair ( = 27, group C). Their median age was 1.9 years (range: 3 months-13.8 years); median weight was 11.4 kg (range: 4.4-39.7 kg). Clinical, electrocardiographic and echocardiographic were available for all patients.

Results: There were no early or late mortality. The mean follow-up duration was 1.8 ± 2.1 years (range: 0.6 months to 8 years). No pulmonary venous obstruction occurred and no residual left-to-right shunts sustained during the follow-up. Reoperation for SVC obstruction was required: 1 (5.3%) in group A, 1 (3.2%) in group B, and 2 (7.4%) in group C ( = 0.78). Four patients (3 in group B, 1 in group C,  = 0.7) presented transient rhythm disturbance at discharge and one patient in group B remains nonsinus rhythm during follow-up. Pacemaker was not required in all patients.

Conclusion: PAPVC to SVC can be safely managed by multiple techniques. Careful manipulation nearby sinus node must be emphasized during double-patch repair to prevent injury of sinus node. Obstruction of postoperative SVC stenosis should be paid attention to after Warden procedure. For young patients, operation should not be performed that early, but until preschool age.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1055/s-0039-1700882DOI Listing
January 2020

Fiber-optic distributed acoustic sensor based on a chirped pulse and a non-matched filter.

Opt Express 2019 Sep;27(20):29415-29424

This paper proposes a distributed acoustic sensor (DAS) scheme, which is immune to the fading problem and can overcome the trade-off existing in traditional ϕ-OTDR between spatial resolution and sensing distance. An optical chirped pulse and non-matched filter method are used, and hence the spatial resolution is mainly determined by the bandwidth of the chirped pulse and non-matched ratio, rather than pulse duration. The Rayleigh interference pattern method is adopted here to quantitatively demodulate strain distribution along the whole sensing fiber, so there is no fading problem, which is a serious problem in the Rayleigh phase method. In proof-of-concept experiments, a DAS with 2-m spatial resolution and 10-km distance range is demonstrated. The response bandwidth of strain is 5 kHz, only limited by the fiber length. A nε-scale strain signal is detected at the far end of fiber with a high SNR of 35 dB.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1364/OE.27.029415DOI Listing
September 2019

Potential of esterase DmtH in transforming plastic additive dimethyl terephthalate to less toxic mono-methyl terephthalate.

Ecotoxicol Environ Saf 2020 Jan 25;187:109848. Epub 2019 Oct 25.

Department of Microbiology, Key Lab of Microbiology for Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China. Electronic address:

Dimethyl terephthalate (DMT) is a primary ingredient widely used in the manufacture of polyesters and industrial plastics; its environmental fate is of concern due to its global use. Microorganisms play key roles in the dissipation of DMT from the environment; however, the enzymes responsible for the initial transformation of DMT and the possible altered toxicity due to this biotransformation have not been extensively studied. To reduce DMT toxicity, we identified the esterase gene dmtH involved in the initial transformation of DMT from the AOPP herbicide-transforming strain Sphingobium sp. C3. DmtH shows 24-41% identity with α/β-hydrolases and belongs to subfamily V of bacterial esterases. The purified recombinant DmtH was capable of transforming DMT to mono-methyl terephthalate (MMT) and potentially transforming other p-phthalic acid esters, including diallyl terephthalate (DAT) and diethyl terephthalate (DET). Using C. elegans as an assay model, we observed the severe toxicity of DMT in inducing reactive oxygen species (ROS) production, decreasing locomotion behavior, reducing lifespan, altering molecular basis for oxidative stress, and inducing mitochondrial stress. In contrast, exposure to MMT did not cause obvious toxicity, induce oxidative stress, and activate mitochondrial stress in nematodes. Our study highlights the usefulness of Sphingobium sp. C3 and its esterase DmtH in transforming p-phthalic acid esters and reducing the toxicity of DMT to organisms.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ecoenv.2019.109848DOI Listing
January 2020

Preferential catabolism of the (S)-enantiomer of the herbicide napropamide mediated by the enantioselective amidohydrolase SnaH and the dioxygenase Snpd in Sphingobium sp. strain B2.

Environ Microbiol 2020 01 14;22(1):286-296. Epub 2019 Nov 14.

Department of Microbiology, Key Lab of Microbiology for Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, 210095, Nanjing, China.

The (R)- and (S)-enantiomers of the chiral herbicide napropamide (NAP) show different biological activities and ecotoxicities. These two enantiomers behave differently in the environment due to enantioselective catabolism by microorganisms. However, the molecular mechanisms underlying this enantioselective catabolism remain largely unknown. In this study, the genes (snaH and snpd) involved in the catabolism of NAP were cloned from Sphingobium sp. B2, which was capable of catabolizing both NAP enantiomers. Compared with (R)-NAP, (S)-NAP was much more rapidly transformed by the amidase SnaH, which initially cleaved the amide bonds of (S)/(R)-NAP to form (S)/(R)-2-(1-naphthalenyloxy)-propanoic acid [(S)/(R)-NP] and diethylamine. The α-ketoglutarate-dependent dioxygenase Snpd, showing strict stereoselectivity for (S)-NP, further transformed (S)-NP to 1-naphthol and pyruvate. Molecular docking and site-directed mutagenesis analyses revealed that when the (S)-enantiomers of NAP and NP occupied the active sites, the distance between the ligand molecule and the coordination atom was shorter than that when the (R)-enantiomers occupied the active sites, which facilitated formation of the transition state complex. This study enhances our understanding of the preferential catabolism of the (S)-enantiomer of NAP on the molecular level.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/1462-2920.14847DOI Listing
January 2020

Xanthoma disseminatum effectively treated with 2-chlorodeoxyadenosin (cladribine): a Chinese case series and mini review of the literature.

Eur J Dermatol 2019 Oct;29(5):538-539

Department of Dermatology, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua University, No 168 Litang Rd, Beijing, 102218, China.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1684/ejd.2019.3629DOI Listing
October 2019

microRNA-218-5p plays a protective role in eosinophilic airway inflammation via targeting δ-catenin, a novel catenin in asthma.

Clin Exp Allergy 2020 01 6;50(1):29-40. Epub 2019 Oct 6.

Division of Respiratory and Critical Care Medicine, Department of Internal Medicine, Tongji Medical College, Tongji Hospital, Huazhong University of Science and Technology, Wuhan, China.

Background: microRNA (miR)-218-5p is involved in cigarette smoke-induced airway inflammation. In our earlier asthma epithelial miRNA profiling data, miR-218-5p was the top 2 down-regulated miRNA. We hypothesize that miR-218-5p plays a role in asthma airway inflammation.

Objective: To unveil the role of miR-218-5p and its target gene in asthma airway inflammation.

Methods: We measured miR-218-5p expression in bronchial brushings of asthma patients (n = 50) and healthy controls (n = 15), and analysed the correlations between miR-218-5p expression and airway eosinophilia. We examined whether CTNND2 was a target of miR-218-5p, and the expression of 12 catenin family members in bronchial brushings, in cultured human bronchial epithelial (HBE) cells and BEAS-2B cells. We explored the role of miR-218-5p-CTNND2 pathway using a murine model of allergic airway inflammation.

Results: Epithelial miR-218-5p expression was significantly decreased and negatively correlated with eosinophils in induced sputum and bronchial biopsies, and other type 2 biomarkers in asthma patients. We verified that CTNND2 (encoding δ-catenin) was a target of miR-218-5p. Remarkably, CTNND2 was the most significantly up-regulated catenin compared with the other 11 catenin family members in bronchial brushings of asthma patients, IL-13-stimulated HBE and BEAS-2B cells. Moreover, epithelial CTNND2 expression positively correlated with airway eosinophilia in asthma. Airway mmu-miR-218-5p expression was also decreased, and Ctnnd2 expression was increased in a murine model of allergic airway inflammation. Intriguingly, mmu-miR-218-5p overexpression suppressed airway hyperresponsiveness, eosinophilic airway inflammation and Ctnnd2 up-regulation in the mouse model. Finally, perturbation of miR-218-5p or CTNND2 expression significantly altered chemokine CCL26 expression in the cell cultures and the mouse model.

Conclusions And Clinical Relevance: Epithelial miR-218-5p plays a protective role in eosinophilic airway inflammation via targeting CTNND2, a novel catenin in asthma, and suppressing chemokine CCL26 expression.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/cea.13498DOI Listing
January 2020

sp. nov., isolated from farmland soil.

Int J Syst Evol Microbiol 2019 Nov;69(11):3443-3447

Jiangsu Key Lab for Solid Organic Waste Utilization, 210095, Nanjing, PR China.

A Gram-stain-negative bacterial strain, designated JW-3, was isolated from a soil sample collected from farmland in Yantai, Shandong Province, PR China. Cells of strain JW-3 are motile rods and strictly aerobic, showing catalase- and oxidase-positive reactions. Strain JW-3 could grow at 16-37 °C (optimum, 30 °C), at pH 6.0-9.0 (pH 7.0) and in the presence of 0-1 % (w/v) NaCl (0.5 %, in Luria-Bertani broth). The major fatty acids were summed feature 3 (C7 and/or C6; 35.5 %), iso-C (16.7 %) and C (10.8 %). The major respiratory quinone was ubiquinone-8 (Q8). The polar lipids of strain JW-3 consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, four unidentified phospholipids, two unidentified lipids, two unidentified glycolipids and a partial unidentified aminophospholipid. Strain JW-3 was most closely related to KA5-B with 97.67 % 16S rRNA gene sequence similarity. Results of phylogenetic analyses, based on 16S rRNA gene sequencing, showed that strain JW-3 forms a distinct phylogenic lineage within the genus of the family . The DNA G+C content of strain JW-3 was 62.57 mol%, based on its draft genome sequence. Average nucleotide identity values and digital DNA-DNA hybridization values for draft genomes, between strain JW-3 and strain KA5-B, were 84.54 and 30.80 %, respectively. Based on its phenotypic, chemotaxonomic and molecular features, and DNA-DNA hybridization results, strain JW-3 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JW-3 (=CCTCC AB 2018184=KCTC 62820).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1099/ijsem.0.003639DOI Listing
November 2019

Baltic amber or Burmese amber: FTIR studies on amber artifacts of Eastern Han Dynasty unearthed from Nanyang.

Spectrochim Acta A Mol Biomol Spectrosc 2019 Nov 12;222:117270. Epub 2019 Jun 12.

Department of Archaeology and Anthropology, University of Chinese Academy of Sciences, Beijing 100049, China; Key Laboratory of Vertebrate Evolution and Human Origin, Chinese Academy of Sciences, Institute of Vertebrate Paleontology and Paleoanthropology, Beijing 100049, China. Electronic address:

There is no case of scientific and technological analysis on archaeological amber of Han Dynasty in China. To estimate the provenance micro-destructively, we have utilized GC/MS (Gas Chromatography-Mass Spectromete) and FTIR (Fourier transform infrared spectroscopy) analysis on the amber artifacts which were excavated from the stone-carved tomb M18 around the 1st century in Nanyang City, Henan Province. GC/MS result suggests that the amber with amount lower than 150 μg is inadequate to detect significant markers. The FTIR spectra have high similarity to Burmese amber without the Baltic shoulder and the absorption peak near 887 cm. From this, it appears that the raw materials of these ambers were from Burma and that Burmese amber was transported to the Central China through Yunnan by free circulation in Han Dynasty.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.saa.2019.117270DOI Listing
November 2019

The role of Wnt7B in the mediation of dentinogenesis via the ERK1/2 pathway.

Arch Oral Biol 2019 Aug 21;104:123-132. Epub 2019 May 21.

State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Cariology and Endodontics, West China Hospital of Stomatology, Sichuan University, No. 14, Section 3, South Renmin Road, Chengdu 610041, PR China. Electronic address:

Objectives: This study investigates the role of Wnt7b in mouse dentin formation.

Design: C57BL/6 mouse tooth germs at different developmental stages were collected to measure the expression of Wnt7b by immunohistochemical staining. The morphology of mandibles of Dmp1-cre;ROSA26-Wnt7b transgenic mice and ROSA26-Wnt7b littermates was analyzed by Micro-CT and HE staining. The ultramicrostructure of dentin was scanned with an electron microscope. Primary mouse dental papillae cells (MDPCs) and odontoblastic cell line (A11) were cultured and infected with adenovirus to overexpress Wnt7b. Cell proliferation and cell apoptosis were evaluated using CCK-8 and flow cytometry. Osteogenic differentiation of MDPCs and A11 was assessed by Alizarin red staining, and qPCR detection of osteogenic gene expression. The activation of signaling pathways was measured by the use of western blot analysis. The ERK1/2 inhibitor was used to test the effect of Wnt7b regulated cell differentiation.

Results: Wnt7b was expressed principally in the mouse odontoblast layer after the early bell stage. In transgenic mice, Wnt7b was over-expressed in tooth mesenchyme, with a thinner predentin layer and thicker intertubular dentin. Both the micro-hardness value and the Ca/Pi ratio of dentin of transgenic mice were higher. Wnt7b promoted proliferation and mineralization of MDPCs and A11. The protein level of p-ERK1/2 was found to be higher in A11 infected with Ad-Wnt7b. The ERK signaling pathway inhibitor partly rescued the Wnt7b-induced differentiation of A11.

Conclusions: Wnt7b enhances dentinogenesis by increasing the proliferation and differentiation of dental mesenchymal cells partly through ERK1/2 pathway.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.archoralbio.2019.05.009DOI Listing
August 2019

Enantioselective Catabolism of Napropamide Chiral Enantiomers in Sphingobium sp. A1 and B2.

J Agric Food Chem 2019 Jun 6;67(24):6819-6827. Epub 2019 Jun 6.

Jiangsu Key Lab for Solid Organic Waste Utilization , Nanjing , Jiangsu 210095 , People's Republic of China.

Napropamide [ N, N-diethyl-2-(1-naphthalenyloxy)propenamide, NAP] is a highly efficient and broad-spectrum amide herbicide. Little is known about the bacterial catabolism of its different enantiomers. Here, we report the isolation of two NAP-degrading strains of Sphingobium sp., A1 and B2, and the different catabolic pathways of different enantiomers in these two strains. Strain A1 dioxygenated NAP at different positions of the naphthalene ring of different enantiomers, leading to the complete degradation of R-NAP while producing a dead-end product from S-NAP. Strain B2 cleaved the amido bonds of both enantiomers, but only the product from S-NAP could be further transformed to form α-naphthol and mineralize in strain B2. The degradation rates of R-NAP and S-NAP in the combination degradation by strains A1 and B2 were 24.8 and 7.5 times that in the single-strain degradation by strain B2 or A1, respectively, showing enhanced synergistic catabolism between strains A1 and B2. This study provides new insights into the enantioselective catabolic network of the chiral herbicide NAP in microorganisms.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.jafc.9b00707DOI Listing
June 2019

Electrospun polycaprolactone/collagen nanofibers cross-linked with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/hydroxysuccinimide and genipin facilitate endothelial cell regeneration and may be a promising candidate for vascular scaffolds.

Int J Nanomedicine 2019 26;14:2127-2144. Epub 2019 Mar 26.

Department of Cardiothoracic Surgery, Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine, Shanghai, China,

Purpose: A promising vascular scaffold must possess satisfying mechanical properties, great hemocompatibility, and favorable tissue regeneration. Combining natural with synthetic materials is a popular method of creating/enhancing such scaffolds. However, the effect of additional modification on the materials requires further exploration.

Materials And Methods: We selected polycaprolactone (PCL), which has excellent mechanical properties and biocompatibility and can be combined with collagen. Electrospun fibers created using a PCL/collagen solution were used to fashion mixed nanofibers, while separate syringes of PCL and collagen were used to create separated nanofibers, resulting in different pore sizes. Mixed and separated nanofibers were cross-linked with glutaraldehyde (GA), 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), and genipin; hence, we named them as mixed GA, mixed EDC (ME), mixed genipin (MG), separated GA, separated EDC (SE), and separated genipin (SG).

Results: Fourier transform infrared (FTIR) spectroscopy and X-ray diffraction showed that cross-linking did not affect the main functional groups of fibers in all groups. ME, MG, SE, and SG met the requisite mechanical properties, and they also resisted collagenase degradation. In hemocompatibility assays, only ME and MG demonstrated ideal safety. Furthermore, ME and MG presented the greatest cytocompatibility. For vascular scaffolds, rapid endothelialization helps to prevent thrombosis. According to human umbilical vein endothelial cell migration on different nanofibers, ME and MG are also successful in promoting cell migration.

Conclusion: ME and MG may be promising candidates for vascular tissue engineering. The study suggests that collagen cross-linked by EDC/-hydroxysuccinimide or genipin facilitates endothelial cell regeneration, which could be of great benefit in tissue engineering of vascular scaffolds.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2147/IJN.S192699DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6440451PMC
May 2019

Chitinophaga deserti sp. nov., isolated from desert soil.

Int J Syst Evol Microbiol 2019 Jun 11;69(6):1783-1788. Epub 2019 Apr 11.

1​Department of Microbiology, Key Laboratory of Microbiology for Agricultural Environment, Ministry of Agriculture College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, PR China.

An aerobic bacterial strain, designated XJ-2, was isolated from a soil sample collected from Gurbantunggut Sandy Desert in PR China. Cells of strain XJ-2 were Gram-stain-negative, non-motile, rod-shaped and non-spore-forming. The new isolate grew well at 10-37 °C (optimum, 28-30 °C), pH 6.0-11.0 (pH 7.0) and 0-1 % (w/v) NaCl (0 %). The 16S rRNA gene sequence of strain XJ-2 showed the highest similarity to that of Chitinophaga rhizosphaerae T16R-86 (99.0 %), followed by Chitinophaga barathri YLT18 (97.0 %), Chitinophagahumicola Ktm-2 (96.7 %) and Chitinophaga niabensis JS13-10 (96.4 %). The major menaquinone of strain XJ-2 was menaquinone 7 and the predominant fatty acids (>5 %) were iso-C15 : 0, C16 : 1ω5c and iso-C17 : 0 3-OH. The polar lipids consisted of phosphatidylethanolamine, an unidentified glycolipid, three unidentified aminolipids and five unidentified lipids. The genome size was 6.33 Mb, comprising 5268 predicted genes with a G+C content of 41.5 mol%. The DNA G+C content was 50.5 mol% based on total genome calculations. The average nucleotide identity and the digital DNA-DNA hybridization values between strain XJ-2 and strain T16R-86 were 79.6 and 22.3 %, respectively. DNA-DNA relatedness between strain XJ-2 and strain YLT18 was 17.0 %. Based on the physiological, biochemical and chemotaxonomic characteristics, strain XJ-2 represents a novel species of the genus Chitinophaga, for which the name Chitinophagadeserti sp. nov. is proposed. The type strain is XJ-2 (KCTC 62443=CCTCC AB 2018019).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1099/ijsem.0.003395DOI Listing
June 2019

Corrigendum to "Transcriptome de novo assembly and analysis of differentially expressed genes related to cytoplasmic male sterility in onion" [Plant Physiol. Biochem. 125 (2018) 35-44].

Plant Physiol Biochem 2018 08 30;129:437. Epub 2018 Jun 30.

Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture, Harbin 150030, China. Electronic address:

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.plaphy.2018.06.038DOI Listing
August 2018

Quality of life of adult vitiligo patients using camouflage: A survey in a Chinese vitiligo community.

PLoS One 2019 24;14(1):e0210581. Epub 2019 Jan 24.

Department of Dermatology, Beijing Tsinghua Changgung Hospital, Tsinghua University, Beijing, China.

Background: Vitiligo is an acquired depigmented skin disease resulting in white macules, which may significantly impair the quality of life (QoL) of the patients.

Objective: To estimate the QoL in Chinese vitiligo patients using camouflage with a more detailed description, and to identify the possible risk factors related to poor QoL.

Methods: An online survey was conducted in vitiligo patients using camouflage from a vitiligo community. Survey questions included demographic, clinical information, dermatology- and vitiligo-specific QoL questionnaires. Multivariate logistic analysis was performed to identify risk factors that related to poor QoL.

Results: In total, 884 respondents were included in the analyses, of which 413 (46.7%) were male. The score of DLQI was 5.83±5.75 (mean± SD). Age, gender, marriage status, occupational status, anogenital involvement, patient-perceived severity (presented by VAS score), symptoms as itching, pain, sunburn and koebner phenomenon, total cost of treatment and degree of satisfaction in camouflage therapy were independently associated with DLQI score (p<0.05).

Conclusion: Vitiligo has considerable impact on QoL of affected patients in Chinese population even when they were using camouflage. Camouflage might be helpful to improve QoL of the patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0210581PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6345473PMC
October 2019

Norepinephrine-functionalised nanoflower-like organic silica as a new adsorbent for effective Pb(II) removal from aqueous solutions.

Sci Rep 2019 01 22;9(1):293. Epub 2019 Jan 22.

School of Port and Transportation Engineering, Zhejiang Ocean University, Zhoushan, 316022, China.

In order to remove Pb(II) ions efficiently from aqueous solutions, a new effective adsorbent of norepinephrine-functionalised nanoflower-like organic silica (NE-NFOS) was synthesised by a biomimetic method. Biomimetic functionalization with norepinephrine has the advantages of environment-friendly and easy operation. Characterization of the NE-NFOS using scanning electron microscopy, transmission electron microscopy, Brunauer-Emmett-Teller method, and Fourier-transform infrared spectroscopy revealed that the NFOS was modified successfully by norepinephrine. Furthermore, the influences of different parameters including adsorption kinetics, solution pH, adsorption isotherms, concentrations of Na, K, Ca, and Mg, desorption and reusability were studied. The adsorption experiments showed that the capacity of NE-NFOS to adsorb Pb(II) ions improved greatly after functionalisation and adsorption equilibrium was attained within 90 min at a pH of 6.0. The Na, K, Ca, and Mg concentrations had little influence on the adsorption, and after recycling for five times, the Pb(II) ion removal efficiency of the adsorbent was more than 79% of its initial value. Thus, it was demonstrated that the NE-NFOS with excellent adsorption performance could be a suitable adsorbent for Pb(II) ions removal in practical applications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-018-36644-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6342944PMC
January 2019

The association between air pollution and preterm birth and low birth weight in Guangdong, China.

BMC Public Health 2019 Jan 3;19(1). Epub 2019 Jan 3.

Research Center for Mental Health and Behavior Big Data, National Research Institute for Family Planning, Beijing, China.

Background: A mountain of evidence has shown that people's physical and mental health can be affected by various air pollutions. Poor pregnancy outcomes are associated with exposure to air pollution. Therefore, this study aims to investigate the association between air pollutions (PM, PM, SO, NO, CO, and O) and preterm birth/low birth weight in Guangdong province, China.

Method: All maternal data and birth data from January 1, 2014 to December 31, 2015 were selected from a National Free Pre-pregnancy Check-ups system, and the daily air quality data of Guangdong Province was collected from China National Environmental Monitoring Center. 1784 women with either preterm birth information (n = 687) or low birth weight information (n = 1097) were used as experimental group. Control group included 1766 women with healthy birth information. Logistic regression models were employed to evaluate the effects of air pollutants on the risk of preterm birth and low birth weight.

Results: The pollution levels of PM, PM, SO, NO, CO, and O in Guangdong province were all lower than the national air pollution concentrations. The concentrations of PM, PM, SO, NO and CO had obvious seasonal trends with the highest in winter and the lowest in summer. O concentrations in September (65.72 μg/m) and October (84.18 μg/m) were relatively higher. After controlling for the impact of confounding factors, the increases in the risk of preterm birth were associated with each 10 μg/m increase in PM (OR 1.043, 95% CI 1.01-1.09) and PM (OR 1.039, 95% CI 1.01~1.14) during the first trimester and in PM (OR 1.038, 95% CI 1.01~1.12), PM (OR 1.024, 95% CI 1.02~1.09), SO (OR 1.081, 95% CI 1.01~1.29), and O (OR 1.016, 95% CI 1.004~1.35) during the third trimester. The increase in the risk of low birth weight was associated with PM, PM, NO, and O in the first month and the last month.

Conclusion: This study provides further evidence for the relationships between air pollutions and preterm birth/low birth weight. Pregnant women are recommended to reduce or avoid exposure to air pollutions during pregnancy, especially in the early and late stages of pregnancy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12889-018-6307-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6318948PMC
January 2019

Clinical efficacy of icotinib in patients with advanced nonsquamous non-small cell lung cancer with unknown EGFR mutation status that failed to respond to second-line chemotherapy.

Ann Transl Med 2018 Oct;6(20):405

Biomedical Engineering, Stony Brook University, Stony Brook, NY, USA.

Background: Evaluation of the clinical efficacy and safety of icotinib in advanced nonsquamous non-small cell lung cancer (NSCLC) patients with an unknown EGFR mutation who failed to respond to second-line chemotherapy.

Methods: Seventy-six cases of advanced nonsquamous NSCLC were involved in this study from seven hospitals from the Hubei province of China. Patients with an unknown EGFR mutation status were treated with Icotinib, at an oral dosage of 125 mg three times daily. All patients were followed up for at least 1 year to observe the efficacy, adverse reactions, and 1-year survival.

Results: The patients' overall objective response rate (ORR) was 34.2%, the disease control rate (DCR) was 75.0%, the clinical benefit rate (CBR) was 80.2%, the median progression-free survival (PFS) was 11.0 months, the median overall survival (OS) was 16.9 months, and the 1-year OS rate was 63.2%. Gender and smoking history were associated with the DCR (P<0.05). Both PFS and OS were significantly higher in groups that had pre-accepted ≤6 cycles of chemotherapy than in groups that had pre-accepted >6 cycles.

Conclusions: Our results demonstrated that icotinib had a better DCR or clinical benefits for treating the patients with unknown EGFR mutation who failed to respond to second-line chemotherapy in advanced nonsquamous NSCLC, and the adverse effects are tolerable.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.21037/atm.2018.09.54DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6230862PMC
October 2018

NUMB maintains bone mass by promoting degradation of PTEN and GLI1 via ubiquitination in osteoblasts.

Bone Res 2018 10;6:32. Epub 2018 Nov 10.

2The Mount Sinai Bone Program, Department of Medicine, Mount Sinai School of Medicine, New York City, NY 10029 USA.

The adaptor protein NUMB is involved in asymmetric division and cell fate determination and recognized as an antagonist of Notch. Previous studies have proved that Notch activation in osteoblasts contributes to a high bone mass. In this study,  however, an osteopenic phenotype was found in 9-week-old mice using osteoblastic specific  to ablate both and its homologue . The trabecular bone mass decreased dramatically while the cortical bone mass was unaffected. Here, the Notch signal was not activated, while the tensin homologue deleted on human chromosome 10 (PTEN), which dephosphorylates phosphatidylinositide 3-kinases, was elevated, attenuating protein kinase B (Akt). The ubiquitination assay revealed that NUMB may physiologically promote PTEN ubiquitination in the presence of neural precursor cell-expressed developmentally downregulated protein 4-1. In addition, the deficiency of / also activated the Hedgehog pathway through GLI1. This process was found to improve the ratio of the receptor activator of nuclear factor-kB ligand to osteoprotegerin, which enhanced the differentiation of osteoclasts and bone resorption . In conclusion, this study provides an insight into  new functons of   NUMB and NUMBL on bone homeostasis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41413-018-0030-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6226489PMC
November 2018

The lead ores circulation in Central China during the early Western Han Dynasty: A case study with bronze vessels from the Gejiagou site.

PLoS One 2018 7;13(11):e0205866. Epub 2018 Nov 7.

Institute of Cultural Relic Research of Nanyang City, Nanyang, Henan, China.

This study first publishes lead isotope data of bronze vessels from Central China in Western Han Dynasty and attempts to find out the lead ores circulation of this time by taking bronzes from the Gejiagou site (Nanyang City, Henan Province) as an example. The elemental concentrations suggest the lead should be introduced on purpose and indicate the provenance information of lead ores. All the lead isotope ratios conform to the characteristics of common lead and most of them are similar to Nanyang local lead ores. The lead of another two bronzes, NY9 and NY13, should be imported from southern China. Combined with the historical background of early Western Han Dynasty, the wider range of the lead ore circulation may be an indicator for, as the loose policy, economic prosperity and transportation improvement.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0205866PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6221295PMC
April 2019

Effects of non-steroidal anti-inflammatory drug-activated gene-1 on Ganoderma lucidum polysaccharides-induced apoptosis of human prostate cancer PC-3 cells.

Int J Oncol 2018 Dec 28;53(6):2356-2368. Epub 2018 Sep 28.

College of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, P.R. China.

Ganoderma lucidum polysaccharides (GLP) has been demonstrated to elicit antitumorigenic and proapoptotic activities in cancer; however, the molecular mechanisms underlying the anticancer effects of GLP have yet to be elucidated. Non-steroidal anti-inflammatory drug-activated gene-1 (NAG-1) has been reported to exert proapoptotic effects and therefore, may serve an important role in cancer prevention. The present study aimed to elucidate the molecular mechanism by which GLP stimulates anticancer activity in human prostate cancer (PCa) PC-3 cells. In addition, the role of NAG-1 in GLP-induced cancer inhibition was examined. The results of the present study demonstrated that GLP significantly inhibited cell viability in a time- and dose-dependent manner in PC-3 cells. Flow cytometry indicated that GLP induced late apoptosis, which was accompanied by poly (ADP-ribose) polymerase 1 (PARP) cleavage, and inhibition of pro-caspase-3, -6 and -9 protein expression. Furthermore, it was observed that the expression levels of NAG-1, and its transcriptional factor early growth response-1, were upregulated in a time- and dose-dependent manner upon GLP treatment. The results of a luciferase assay demonstrated that GLP induced the promoter activity of NAG-1, thus indicating that NAG-1 may be transcriptionally regulated by GLP. The secretion of NAG-1 proteins into the cell culture medium was also upregulated upon GLP treatment. Furthermore, inhibition of NAG-1 expression by small interfering RNA significantly, but not completely, prevented GLP-induced apoptosis, and reversed the effects of GLP on PARP and pro-caspase expression. It was further demonstrated that GLP inhibited the phosphorylation of protein kinase B and mitogen-activated protein kinase/extracellular signal-regulated kinase signaling in PC-3 cells. The present study is the first, to the best of our knowledge, to report that GLP may induce apoptosis of PCa cells, which is partially mediated through NAG-1 induction. The present findings may be helpful in elucidating the anticancer mechanisms of GLP through NAG-1 induction for its chemopreventive potential in PCa.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3892/ijo.2018.4578DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6203158PMC
December 2018

Chitinophaga parva sp. nov., a new member of the family Chitinophagaceae, isolated from soil in a chemical factory.

Int J Syst Evol Microbiol 2018 Nov 12;68(11):3452-3457. Epub 2018 Sep 12.

2​Jiangsu Key Lab for Solid Organic Waste Utilization, 210095 Nanjing, PR China.

A Gram-stain-negative bacterium, designated LY-1, was isolated from the soil sample collected from a chemical factory in Fuyang city, Anhui province, China. Cells of strain LY-1 were strictly aerobic, non-motile and rod-shaped. Strain LY-1 grew optimally at pH 7.0 and at 30-35 °C. The taxonomic position was investigated using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain LY-1 was a member of the genus Chitinophaga and showed the highest sequence similarity to Chitinophaga costaii A37T2 (97.5 %) and lower (<97.0 %) sequence similarity to other known Chitinophaga species. Chemotaxonomic analysis revealed that strain LY-1 possessed menaquinone-7 as the major isoprenoid quinone; and iso-C15 : 0 (46.4 %), C16 : 1ω5c (27.8 %) and iso-C17 : 0 3-OH (9.0 %) were the predominant fatty acids. The polar lipids of strain LY-1 consisted of phosphatidylethanolamine, three unidentified phosphoaminolipids, one unidentified phospholipid, four unidentified lipids, two unidentified aminolipids and two unidentified glycolipids. The genomic DNA G+C content of strain LY-1 was 52.4 mol% based on total genome calculations. The average nucleotide identity and the digital DNA-DNA hybridization value of the draft genomes between strain LY-1 and strain A37T2 were 76.8 and 19.8 %, respectively. Based on the phylogenetic and phenotypic characteristics, chemotaxonomic data, and DNA-DNA hybridization, strain LY-1 is considered a novel species of the genus Chitinophaga, for which the name Chitinophagaparva sp. nov. (type strain LY-1=CCTCC AB 2018018=KCTC 62444) is proposed.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1099/ijsem.0.003006DOI Listing
November 2018

High-fidelity distributed fiber-optic acoustic sensor with fading noise suppressed and sub-meter spatial resolution.

Opt Express 2018 Jun;26(13):16138-16146

In order to solve fading problem and realize sub-meter spatial resolution in DAS, this paper proposes a novel configuration of time-gated digital optical frequency domain reflectometry (TGD-OFDR) based on optical intensity modulator (IM). IM has a large modulation bandwidth and the positive and negative harmonics can be fully used to suppress fading while the spatial resolution remains unchanged. In experiments, with fading suppressed, the spatial resolution of DAS is 0.8 m and the strain resolution is about 245.6 pε√Hz along the total 9.8-km sensing fiber. The response bandwidth of vibration is 5 kHz, only/limited by the fiber length.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1364/OE.26.016138DOI Listing
June 2018