Publications by authors named "Di Zheng"

55 Publications

Synergistic Anticancer Effects of Cisplatin Combined with Combretastatin A4 Phosphate on Human Osteosarcoma-Xenografted Mice.

Cells Tissues Organs 2021 Aug 25:1-8. Epub 2021 Aug 25.

Department of Trauma Surgery, Wuhan No. 1 Hospital, Wuhan, China.

This study aimed to investigate the effectiveness of anticancer therapy combining a cytotoxic chemotherapeutic agent, cisplatin (DDP), and a vascular disruptive drug, combretastatin A4 phosphate (CA4P), in osteosarcoma. First, a human osteosarcoma-xenografted mice model was established. Second, the transplanted tumor models were treated with DDP and CA4P in combination or as monotherapy. Third, the therapeutic effects and the mechanism of the drug combination in the inhibition of transplanted tumors was studied. Finally, the toxic effects of the drugs were observed and recorded. The results showed that DDP combined with CA4P significantly inhibited the growth and lung metastasis of transplanted tumors compared with the monotherapy drug group and vehicle control group. Histopathological analysis revealed that apoptotic and necrotic cell death significantly increased in the combination group, and combined treatment significantly inhibited the proliferation of osteosarcoma cells compared with either agent alone or the vehicle control. Additionally, no obvious toxic effects were observed in the combination group. These results indicate that the combined effects of DDP and CA4P on the progression of human osteosarcoma in vivo were superior to that of either agent alone. DDP combined with CA4P exerted synergistic effects at lower concentrations and promoted apoptosis and necrosis, as well as inhibited proliferation of osteosarcoma cells, but it did not increase the systemic toxic effects of chemotherapy. Our findings highlight CA4P as an effective anticancer agent candidate for combination with DDP in clinical applications to treat osteosarcoma.
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http://dx.doi.org/10.1159/000517446DOI Listing
August 2021

Temperature-insensitive curvature sensor based on Bragg gratings written in strongly coupled multicore fiber.

Opt Lett 2021 Aug;46(16):3933-3936

A novel temperature-insensitive optical curvature sensor has been proposed and demonstrated. The sensor is fabricated by inscribing fiber Bragg gratings with short lengths into a piece of strongly coupled multicore fiber (SCMCF) and spliced to the conventional single-mode fiber. Due to the two supermodes being supported by the SCMCF, two resonance peaks, along with a deep notch between them, were observed in the reflection spectrum. The experimental results show that the depth of the notch changes with the curvature with a sensitivity up to 15.9/ in a lower curvature range. Besides, thanks to the unique property of the proposed sensor, the notch depth barely changes with temperature. Based on the intensity demodulation of the notch depth, the temperature-insensitive curvature sensor is achieved with the cross sensitivity between the temperature, and the curvature is as low as 0.001/.
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http://dx.doi.org/10.1364/OL.432889DOI Listing
August 2021

A Novel Six Metastasis-Related Prognostic Gene Signature for Patients With Osteosarcoma.

Front Cell Dev Biol 2021 23;9:699212. Epub 2021 Jul 23.

Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan, China.

Osteosarcoma is the most common malignant bone tumor, and although there has been significant progress in its management, metastases often herald incurable disease. Here we defined genes differentially expressed between primary and metastatic osteosarcoma as metastasis-related genes (MRGs) and used them to construct a novel six-MRG prognostic signature for overall survival of patients with osteosarcoma. Validation in internal and external datasets confirmed satisfactory accuracy and generalizability of the prognostic model, and a nomogram based on the signature and clinical variables was constructed to aid clinical decision-making. Of the six MRGs, is a well-documented tumor suppressor gene that is poorly defined in osteosarcoma. Consistent with tumor suppressor function, FHIT was downregulated in osteosarcoma cells and human osteosarcoma samples. FHIT overexpression inhibited osteosarcoma proliferation, migration, and invasion both and . Mechanistically, FHIT overexpression upregulate the epithelial marker E-cadherin while repressing the mesenchymal markers N-cadherin and vimentin. Our six-MRG signature represents a novel and clinically useful prognostic biomarker for patients with osteosarcoma, and FHIT might represent a therapeutic target by reversing epithelial to mesenchymal transition.
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http://dx.doi.org/10.3389/fcell.2021.699212DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8343004PMC
July 2021

Mouse strain-specific responses of mitochondrial respiratory function and cardiac hypertrophy to isoproterenol treatment.

Sheng Li Xue Bao 2021 Jun;73(3):459-470

Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan 430060, China.

Cardiac hypertrophy is a common pathological process of various cardiovascular diseases and eventually develops into heart failure. This paper was aimed to study the different pathological characteristics exhibited by different mouse strains after hypertrophy stimulation. Two mouse strains, A/J and FVB/nJ, were treated with isoproterenol (ISO) by osmotic pump to induce cardiac hypertrophy. Echocardiography was performed to monitor heart morphology and function. Mitochondria were isolated from hearts in each group, and oxidative phosphorylation function was assayed in vitro. The results showed that both strains showed a compensatory enhancement of heart contractile function after 1-week ISO treatment. The A/J mice, but not the FVB/nJ mice, developed significant cardiac hypertrophy after 3-week ISO treatment as evidenced by increases in left ventricular posterior wall thickness, heart weight/body weight ratio, cross sectional area of cardiomyocytes and cardiac hypertrophic markers. Interestingly, the heart from A/J mice contained higher mitochondrial DNA copy number compared with that from FVB/nJ mice. Functionally, the mitochondria from A/J mice displayed faster O consumption at state III with either complex I substrates or complex II substrate, compared with those from FVB/nJ mice. ISO treatment did not affect mitochondrial respiratory control rate (RCR), but significantly suppressed the ADP/O ratio generated from the complex II substrate in both strains. The ADP/O ratio generated from the complex I substrates in A/J mice declined by 50% after ISO treatment, whereas FVB/nJ mice were not affected. These results suggest that, compared with FVB/nJ mice, A/J mice possesses a poor integrity of mitochondrial respiratory chain that might contribute to its vulnerability to ISO-induced cardiac hypertrophy.
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June 2021

Laser-Scanning-Guided Assembly of Quasi-3D Patterned Arrays of Plasmonic Dimers for Information Encryption.

Adv Mater 2021 Jun 10;33(24):e2100325. Epub 2021 May 10.

State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Fudan University, Shanghai, 200438, China.

The application of plasmonic dimeric nanostructures in color displays, data storage, and especially metamaterials necessitates the patterning of dimers into ordered arrays, but controllable assembly of plasmonic nanoparticles into patterned dimer arrays on substrates still remains a challenge. Here, a facile laser-scanning-based strategy to fabricate quasi-3D patterned arrays of plasmonic nanoparticle dimers with controlled orientation for plasmonic information encryption is reported. Laser scanning of polymer-covered plasmonic nanoparticle (e.g., gold) arrays selectively exposes the surface of irradiated nanoparticle via localized photothermal heating, guiding the assembly of another type of nanoparticles onto the exposure nanoparticle surface to form dimers on substrates. This combined top-down/bottom-up approach is highly flexible in forming high-resolution patterns of plasmonic dimers from nanoparticles of different sizes and shapes. The z-axis orientation, interparticle spacing, and nanoparticle size and shape of plasmonic dimers can be precisely tuned, enabling the modulation of the coupled resonances of the dimer arrays. Moreover, it is demonstrated that the patterned dimer arrays can be used in information encryption where their plasmonic color can be repeatedly displayed and erased. This work provides an important addition to tools for the fabrication of patterned complex plasmonic nanostructures from as-synthesized nanoparticles with broad applications.
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http://dx.doi.org/10.1002/adma.202100325DOI Listing
June 2021

Inhibition of the Notch signaling pathway attenuates progression of cell motility, metastasis, and epithelial-to-mesenchymal transition-like phenomena induced by low concentrations of cisplatin in osteosarcoma.

Eur J Pharmacol 2021 May 21;899:174058. Epub 2021 Mar 21.

Department of Trauma Surgery, Wuhan No. 1 Hospital, Wuhan, 430022, Hubei, China.

Although advances in osteosarcoma treatment have been made in recent decades, the survival rate for patients suffering from metastatic disease, especially lung metastasis, remains disappointing. Previous studies have confirmed that epithelial-to-mesenchymal transition (EMT) is associated with tumor metastasis, and several studies have suggested that osteosarcoma cells also exhibit EMT-like characteristics. In addition, Notch signaling is known to be related to the development and progression of human malignancies, including osteosarcoma. However, whether chemotherapy affects the EMT-like events and whether these events are medicated by Notch signaling remain to be elucidated. To address these issues, in the current work, osteosarcoma 143B cells were exposed to sublethal concentrations of the first-line chemotherapeutic agent cisplatin (DDP), which promoted cell migration, in vitro invasion, and in vivo lung metastasis. Furthermore, low concentrations of DDP upregulated mesenchymal phenotype-related genes and proteins and promoted EMT-like properties in osteosarcoma cells. In addition, low concentrations of DDP could activate the Notch receptor and its target genes. Finally, combined treatment of DDP with the Notch signaling pathway inhibitor DAPT, which can effectively downregulate mesenchymal phenotype-related genes and proteins, inhibited cell migration and invasion in vitro, and it decreased pulmonary metastatic nodules in vivo. The results of the current study supported the idea that low concentrations of DDP could induce EMT-like characteristics in osteosarcoma cells and could promote cell mobility in vitro, as well as pulmonary metastasis in vivo. Importantly, however, these biological processes are mediated by the Notch signaling pathway. Blocking the Notch signaling pathway can effectively attenuate the osteosarcoma EMT-like phenotype and its associated migration, invasion, and metastasis.
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http://dx.doi.org/10.1016/j.ejphar.2021.174058DOI Listing
May 2021

EZH2 Dynamically Associates With Non-coding RNAs in Mouse Hearts After Acute Angiotensin II Treatment.

Front Cardiovasc Med 2021 25;8:585691. Epub 2021 Feb 25.

Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China.

Enhancer of zeste 2 (EZH2) governs gene reprogramming during cardiac hypertrophy through epigenetic remodeling, a process regulated by numerous non-coding RNAs (ncRNAs). However, the dynamic interaction between EZH2 and ncRNAs upon hypertrophic stimulation remains elusive. Here we performed an unbiased profiling for EZH2-associated ncRNAs in mouse hearts treated with Angiotensin II (AngII) at different time points (0, 4, and 24 h). The interactions between EZH2 and long ncRNAs (lncRNAs), Chaer, Mirt1, Hotair, and H19, were validated by PCR. RIP-seq analysis identified a total of 126 ncRNAs to be significantly associated with EZH2. These ncRNAs covers all five categories including intergenic, antisense, intron-related, promoter-related and both antisense and promoter-related. According to their changing patterns after AngII treatment, these ncRNAs were clustered into four groups, constantly enhanced, transiently enhanced, constantly suppressed and transiently suppressed. Structural prediction showed that EZH2 bound to hairpin motifs in ncRNAs including snoRNAs. Interaction strength prediction and RNA pull-down assay confirmed the direct interaction between EZH2 and Snora33. Interestingly, two antisense lncRNAs of Malat1, Gm20417, and Gm37376, displayed different binding patterns from their host gene after AngII treatment, suggesting a crucial role of this genomic locus in modulating EZH2 behavior. Our findings reveal the profile of EZH2-associated ncRNAs upon hypertrophic stimulation, and imply a dynamic regulation of EZH2 function in cardiac hypertrophy.
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http://dx.doi.org/10.3389/fcvm.2021.585691DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7959742PMC
February 2021

Impact of small and dense low-density lipoprotein (sd-LDL)on contrast-induced acute kidney injury in patients with acute ST-segment elevation myocardial infarction undergoing primary percutaneous coronary intervention.

Int Urol Nephrol 2021 Mar 6. Epub 2021 Mar 6.

Institute of Cardiovascular Diseases, Xuzhou Medical University, Xuzhou, 221000, China.

Objective: To investigate the impact of serum small and dense low-density lipoprotein (sd-LDL) on contrast-induced acute kidney injury (CI-AKI) after emergency percutaneous coronary intervention (PCI) in patients with acute ST-segment elevation myocardial infarction (STEMI).

Method: From November 2019 to August 2020, 352 patients with STEMI who underwent primary PCI were recruited consecutively. Patients were divided into CI-AKI group (n = 71) and non-CI-AKI group (n = 281). CI-AKI was defined as an increase in serum creatinine (≥ 25% or ≥ 0.5 mg/dL) from baseline occurring 72 h after PCI. All subjects were tested for sd-LDL.

Results: In the 352 eligible patients with STEMI receiving emergency PCI, 71 patients (20.2%) developed CI-AKI. The levels of sd-LDL in CI-AKI group was higher than those in the non-CI-AKI group, and the difference was statistically significant (P < 0.05). The area under the curve (AUC) of the sd-LDL was 0.741 [95% confidence interval (CI) 0.538-0.636] in the STEMI patients receiving emergency PCI. CI-AKI model included the following five predictors: sd-LDL, NLR, Diabetes, Pre-PCI eGFR, and Log NT-proBNP. The AUC of forecast probability was 0.835 [95% confidence interval (CI) 0.786-0.883].The Hosmer-Lemeshow test has a P value of 0.519, which confirms the model's goodness of fit.

Conclusion: Increased sd-LDL is independently associated with risk of CI-AKI in STEMI patients treated by primary PCI.
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http://dx.doi.org/10.1007/s11255-021-02821-xDOI Listing
March 2021

Strong plasmon-exciton coupling in transition metal dichalcogenides and plasmonic nanostructures.

Nanoscale 2021 Mar;13(8):4408-4419

Institute of Microscale Optoelectronics, Shenzhen University, Shenzhen 518060, China. and The Institute for Advanced Studies, Wuhan University, Wuhan 430072, China and School of Physics and Technology, Center for Nanoscience and Nanotechnology, and Key Laboratory of Artificial Micro- and Nano-structures of Ministry of Education, Wuhan University, Wuhan 430072, China.

Achieving strong coupling between emitters and cavity photons holds an important position in the light-matter interaction due to its applications such as polariton lasing, all-optical switches, and quantum information processing. However, room-temperature polaritonic devices with subwavelength dimensions based on strong light-matter coupling are difficult to realize using traditional emitter-cavity coupled systems. In recent years, coupled systems constructed from plasmonic nanostructures and transition metal dichalcogenides (TMDs) have shown their potential in achieving room-temperature strong coupling and robustness in the nanofabrication processes. This minireview presents the recent progress in strong plasmon-exciton coupling in such plasmonic-TMD hybrid structures. Differing from a broader scope of strong coupling, we focus on the plasmon-exciton coupling between excitons in TMDs and plasmons in single nanoparticles, nanoparticle-over-mirrors, and plasmonic arrays. In addition, we discuss the future perspectives on the strong plasmon-exciton coupling at few-excitons level and the nonlinear response of these hybrid structures in the strong coupling regime.
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http://dx.doi.org/10.1039/d0nr08592hDOI Listing
March 2021

Catalytic ozonation treatment of papermaking wastewater by Ag-doped NiFeO: Performance and mechanism.

J Environ Sci (China) 2020 Nov 7;97:75-84. Epub 2020 Jun 7.

Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, Ministry of Education, Hohai University, Nanjing 210098, China; College of Environment, Hohai University, Nanjing 210098, China. Electronic address:

The catalytic ozonation treatment of secondary biochemical effluent for papermaking wastewater by Ag-doped nickel ferrite was investigated. Ag-doped catalysts prepared by sol-gel method were characterized, illustrating that Ag entirely entered the crystalline of NiFeO and changed the surface properties. The addition of catalyst enhanced the removal efficiency of chemical oxygen demand and total organic carbon. The results of gas chromatography-mass spectrometer, ultraviolet light absorbance at 254 nm and three-dimensional fluorescence excitation-emission matrix suggested that aromatic compounds were efficiently degraded and toxic substances, such as dibutyl phthalate. In addition, the radical scavenging experiments confirmed the hydroxyl radicals acted as the main reactive oxygen species and the surface properties of catalysts played an important role in the reaction. Overall, this work validated potential applications of Ag-doped NiFeO catalyzed ozonation process of biologically recalcitrant wastewater.
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http://dx.doi.org/10.1016/j.jes.2020.04.014DOI Listing
November 2020

Predictive value of combining the level of fibrinogen and antithrombin III for contrast-induced nephropathy in coronary artery disease patients undergoing percutaneous coronary intervention.

Biomed Rep 2020 Oct 22;13(4):26. Epub 2020 Jul 22.

Department of Cardiology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu 221002, P.R. China.

Contrast-induced nephropathy (CIN) is a common adverse event in the diagnosis and treatment of coronary intervention. The current study investigated the predictive effect of preoperative fibrinogen (FIB) combined with antithrombin III (AT-III) on CIN following percutaneous coronary intervention (PCI). A total of 394 patients who underwent PCI between October 2018 and May 2019 were selected for the present study. Pre-procedural FIB levels and AT-III activity were measured before PCI. CIN was defined as a 0.5 mg/dl or 25% increase in serum creatinine levels 48 to 72 h after exposure to a radiocontrast agent. Patients were classified into CIN and non-CIN groups. CIN occurred in 48 (12.2%) patients. The serum FIB levels were significantly higher in patients who developed CIN compared with those who did not develop CIN. In addition, AT-III levels ≤89.5% were associated with higher rates of CIN. Logistical regression analysis showed that high FIB, and low AT-III and albumin levels were high-risk factors associated with CIN. For FIB, the area under the receiver operating characteristic curve (AUC) for predicting CIN was 0.653. The optimal cut-off value was 3.48 g/l with a sensitivity of 45.8% and a specificity of 79.7% [95% confidence interval (CI): 0.603-0.701; P=0.0002)]. For AT-III, the AUC was 0.711, and the optimal cut-off value was 89.5%, with a sensitivity of 81.3% and specificity of 58.2% (95% CI: 0.659-0.758; P<0.0001). When combining FIB and AT-III, the AUC was 0.747. The optimal cut-off value was 0.090424, with a diagnostic sensitivity of 93.8% and specificity of 46.6% (95% CI: 0.697-0.792; P<0.0001). The results showed that FIB combined with AT-III resulted in improved predictive accuracy of CIN (FIB vs. AT-III, AUC=0.653 vs. 0.711, P=0.292; FIB vs. FIB + AT-III, AUC=0.653 vs. 0.747, P=0.012; AT-III vs. FIB + AT-III, AUC=0.711 vs. 0.747, P=0.138). Pre-procedural levels of FIB, AT-III and albumin were independently associated with an increased risk of CIN. Furthermore, the results suggested that the combination of FIB and AT-III was a better predictor of CIN after PCI.
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http://dx.doi.org/10.3892/br.2020.1333DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7403811PMC
October 2020

Flexible Photothermal Assemblies with Tunable Gold Patterns for Improved Imaging-Guided Synergistic Therapy.

Small 2020 08 21;16(34):e2002790. Epub 2020 Jul 21.

State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, 100029, China.

Self-assembly of gold nanoparticles demonstrates a promising approach to realize enhanced photoacoustic imaging (PAI) and photothermal therapy (PTT) for accurate diagnosis and efficient cancer therapy. Herein, unique photothermal assemblies with tunable patterns of gold nanoparticles (including arcs, rings, ribbons, and vesicles) on poly(lactic-co-glycolic acid) (PLGA) spheres are constructed taking advantage of emulsion-confined and polymer-directed self-assembly strategies. The influencing factors and formation mechanism to produce the assemblies are investigated in details. Both the emulsion structure and migration behaviors of amphiphilic block copolymer tethered gold nanoparticles are found to contribute to the formation of versatile photothermal assemblies. Hyaluronic acid-modified R-PLGA-Au (RPA) exhibits outstanding photothermal performances under NIR laser irradiation, which is induced by strong plasmonic coupling between adjacent gold nanoparticles. It is interesting that secondary assembly of RPA can be triggered by NIR laser irradiation. Prolonged residence time in tumors is achieved after RPA assemblies are fused into superstructures with larger sizes, realizing real-time monitoring of the therapeutic processes via PAI with enhanced photoacoustic signals. Notably, synergistic effect resulting from PTT-enhanced chemotherapy is realized to demonstrate high antitumor performance. This work provides a facile strategy to construct flexible photothermal assemblies with favorable properties for imaging-guided synergistic therapy.
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http://dx.doi.org/10.1002/smll.202002790DOI Listing
August 2020

Catalytic ozonation of dibutyl phthalate in the presence of Ag-doped NiFeO and its mechanism.

Environ Technol 2020 Jun 16:1-11. Epub 2020 Jun 16.

Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, Ministry of Education, Hohai University, Nanjing, People's Republic of China.

In this study, NiFeO and AgNiFeO were successfully prepared by the sol-gel method and applied to catalyze ozone for dibutyl phthalate (DBP) degrading. The synthesized catalysts were characterized by X-ray diffraction, Fourier-transform infrared spectroscopy, and vibrating sample magnetometer. The removal efficiency was compared with different catalyst dosage, pH condition, and initial DBP concentration for the two catalysts. The results showed that the introduction of AgNiFeO enhanced the ozone decomposition and the utilization efficiency of ozone from 32.2% (ozone alone) to 56.5% and improved the degradation efficiency of DBP significantly. The two catalysts both presented good recycling performance. Furthermore, the scavenger test displayed DBP degradation by catalytic ozonation dominated by OH in this study. These insights into the catalytic ozonation mechanism on AgNiFeO will advance their practical application to the catalytic degradation of organic pollutants.
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http://dx.doi.org/10.1080/09593330.2020.1770338DOI Listing
June 2020

Circulating tumor DNA clearance predicts prognosis across treatment regimen in a large real-world longitudinally monitored advanced non-small cell lung cancer cohort.

Transl Lung Cancer Res 2020 Apr;9(2):269-279

Burning Rock Biotech, Guangzhou 510300, China.

Background: Although growth advantage of certain clones would ultimately translate into a clinically visible disease progression, radiological imaging does not reflect clonal evolution at molecular level. Circulating tumor DNA (ctDNA), validated as a tool for mutation detection in lung cancer, could reflect dynamic molecular changes. We evaluated the utility of ctDNA as a predictive and a prognostic marker in disease monitoring of advanced non-small cell lung cancer (NSCLC) patients.

Methods: This is a multicenter prospective cohort study. We performed capture-based ultra-deep sequencing on longitudinal plasma samples utilizing a panel consisting of 168 NSCLC-related genes on 949 advanced NSCLC patients with driver mutations to monitor treatment responses and disease progression. The correlations between ctDNA and progression-free survival (PFS)/overall survival (OS) were performed on 248 patients undergoing various treatments with the minimum of 2 ctDNA tests.

Results: The results of this study revealed that higher ctDNA abundance (P=0.012) and mutation count (P=8.5×10) at baseline are associated with shorter OS. We also found that patients with ctDNA clearance, not just driver mutation clearance, at any point during the course of treatment were associated with longer PFS (P=2.2×10, HR 0.28) and OS (P=4.5×10, HR 0.19) regardless of type of treatment and evaluation schedule.

Conclusions: This prospective real-world study shows that ctDNA clearance during treatment may serve as predictive and prognostic marker across a wide spectrum of treatment regimens.
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http://dx.doi.org/10.21037/tlcr.2020.03.17DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7225135PMC
April 2020

Temperature-insensitive optical tilt sensor based on a single eccentric-core fiber Bragg grating.

Opt Lett 2019 Nov;44(22):5570-5573

This Letter presents a simple temperature-insensitive optical tilt sensor based on a single eccentric-core fiber Bragg grating (ECFBG). By partly inserting an ECFBG into a ceramic ferrule, the reflection spectrum of the ECFBG splits into two peaks as a result of the applied tilt angle. The magnitude and direction of inclination in one dimension can be determined by monitoring the wavelength separation between both peaks, which is inherently insensitive to temperature. The proposed tilt sensor has a good linear response within a wide dynamic range of ±45°, with a sensitivity of 0.012 nm/°, with a resolution of 0.83°, and with an accuracy of 0.41°. Being free from any inherent mechanical joint/friction, along with the advantages of a compact structure, good repeatability, and low cost, the proposed sensor is highly suitable for practical engineering applications.
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http://dx.doi.org/10.1364/OL.44.005570DOI Listing
November 2019

A pilot RCT of a school nurse delivered intervention to reduce student anxiety.

J Clin Child Adolesc Psychol 2021 Mar-Apr;50(2):177-186. Epub 2019 Aug 2.

Department of Statistics, University of Connecticut.

The goal of this study was to evaluate the feasibility and impact of brief school-nurse-administered interventions for reducing anxiety. Thirty school nurses in Connecticut and Maryland were randomly assigned to deliver the Child Anxiety Learning Modules (CALM; = 14) or CALM-Relaxation only (CALM-R; = 16). Students ( = 54) were ages 5-12 ( age = 8; 84.9% White; 68.5% female) with elevated anxiety symptoms and/or anxiety disorders. Feasibility was assessed based on recruitment, retention, attendance, training and intervention satisfaction, and intervention adherence. Multiple informants, including independent evaluators (IEs), completed measures of clinical improvement at postintervention and at a 3-month follow-up. Of nurses in CALM and CALM-R, 62% and 81%, respectively, enrolled a student and completed an average of 6 sessions. Youth retention was 85% and 94% in CALM and CALM-R, respectively. Training and intervention satisfaction were high. At postintervention and follow-up, youth in both groups showed significant reductions in anxiety and related symptoms and improvements in functioning. Within-group effect sizes were medium to large, and between-group effect sizes were small. Task shifting responsibility for delivering brief mental health interventions to school nurses is feasible and shows promise for reducing anxiety and related impairment. This approach may also be integrated within a response to intervention model used in schools.: Brief school-nurse-administered anxiety reduction interventions were shown to be feasible and had a positive impact on student anxiety and related impairment highlighting that school nurses can be an important school resource.
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http://dx.doi.org/10.1080/15374416.2019.1630833DOI Listing
August 2019

[Simultaneous determination of 33 hair dyes in oxidative hair dye products by high performance liquid chromatography].

Se Pu 2019 Jul;37(7):759-765

Beijing Institute for Drug Control, Beijing 102206, China.

A method was developed for the simultaneous determination of 33 hair dyes in oxidative hair dye products by high-performance liquid chromatography (HPLC). The analytes were separated on a Waters Atlantis T3 MV Kit column (250 mm×4.6 mm, 5 μm) by gradient elution using phosphate buffer-acetonitrile as mobile phases. The components were detected at two different wavelengths of 235 and 280 nm using a diode array detector. The results showed that the method had good linearity with correlation coefficients exceeding 0.999. Precision was good for the 33 analytes, with the relative standard deviations (RSDs) less than 2%. Stabilities of tetraaminopyrimidine sulfate and 2,4-diaminophenoxyethanol HCl in 12 h, as well as other analytes in 24 h, were good, with RSDs less than 5%. Recoveries of the method ranged from 77.6% to 116.3% at three different concentration levels. The method proved to be simple, rapid, and accurate, suitable for the determination of various hair dyes in oxidative hair dye products.
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http://dx.doi.org/10.3724/SP.J.1123.2019.01043DOI Listing
July 2019

Effect of astaxanthin on apoptosis of rat renal tubular epithelial cells induced by iohexol.

Am J Transl Res 2019 15;11(5):3039-3047. Epub 2019 May 15.

Department of Cardiology, Affiliated Hospital of Xuzhou Medical University Xuzhou 221000, Jiangsu, PR China.

Contrast acute kidney injury refers to acute renal failure due to the application of contrast agents. Astaxanthin, as an antioxidant, can improve early acute kidney injury in severely burned rats. However, the mechanism of astaxanthin for renal protection is still unclear. In this study, the rat renal tubular epithelial cells (NRK-52E) were treated with iohexol, astaxanthin, astaxanthin plus nicotinamide and nicotinamide. Subsequently, the nuclear morphology was observed by fluorescence staining of DAPI DNA, the apoptosis was detected by flow cytometry, the mitochondrial membrane potential was detected by JC-1 and the SIRT1, P53, Bax, Bcl-2 protein expression level was detected by Western blotting. We found that astaxanthin can reduce nuclear pyknosis and nuclear deep staining, decrease the number of apoptotic cells, up-regulate the expression of proapoptotic proteins P53 and Bax and up-regulate the expression of anti-apoptotic protein Bcl-2 by increasing SIRT1 expression level, thereby exerting protective effects on renal tubular epithelial cells. At the same time, nicotinamide has the opposite effect on the NRK-52E compared with astaxanthin. These results indicated that astaxanthin may provide a new option for the prevention of contrast-induced acute kidney injury.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6556630PMC
May 2019

The compound mutations in Chinese advanced non-small cell lung cancer patients.

Cancer Biol Ther 2019 16;20(8):1097-1104. Epub 2019 Apr 16.

a Department of Respiratory Medicine , Center for Molecular Medicine, Xiangya Hospital , Central South University , Changsha , China.

Literatures regarding the prevalence and clinical significance of compound EGFR mutations are limited. Until now, none of retrospective or prospective research has focused on compound mutations except case reports. In this study, we screened a cohort of 3,000 treatment-naïve Chinese advanced NSCLC patients using capture-based ultra-deep targeted sequencing to evaluate the prevalence of compound mutations and the efficacy of EGFR-TKI in this population. Of the 3,000 patients screened, 1,266 (42.2%) had mutation; among them, 15 patients (1.2%) harborin compound mutations, with 10 patients carrying L858R in combination with a rare mutation and five patients carrying two rare mutations. No patient with 19del was observed. Interestingly, no configuration was identified in this cohort. All of the patients harborin compound mutations were non-smokers, histologically diagnosed with adenocarcinoma and received first-generation EGFR-TKI. Furthermore, our data also revealed that patients with compound mutations exhibit comparable PFS to first generation EGFR-TKI comparing to patients with single activating mutation. This observation was further supported by molecular modeling analyses which demonstrated compound mutations do not alter the ATP-binding pocket of , thus having no effect on the interaction between gefitinib and .
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http://dx.doi.org/10.1080/15384047.2019.1595280DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6605978PMC
August 2020

Publisher Correction: Cannabinoid CB receptors in the amygdalar cholecystokinin glutamatergic afferents to nucleus accumbens modulate depressive-like behavior.

Nat Med 2019 Feb;25(2):350

Center for Neuroscience and Department of Neurology of Second Affiliated Hospital, NHC and CAMS Key Laboratory of Medical Neurobiology, Joint Institute for Genetics and Genome Medicine between Zhejiang University and University of Toronto, Zhejiang University School of Medicine, Hangzhou, China.

In the version of this article originally published, there were several errors in Fig. 4. In Fig. 4a, the title read '3D repeated optical inhibition after CSDS.' It should have read '3-day repeated optical inhibition after CSDS.' In Fig. 4c, two labels that should have been aligned with the time axis appeared in the wrong place in the figure. The ticks labeled 'SI' and 'Fiber implant' should have also been labeled with '10' and '14,' respectively. Additionally, in Fig. 4j, a label that should have been aligned with the time axis appeared in the wrong place in the figure. The tick labeled 'Fiber implant' should have also been labeled with '14.' The errors have been corrected in the print, PDF and HTML versions of the manuscript.
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http://dx.doi.org/10.1038/s41591-019-0372-zDOI Listing
February 2019

Cannabinoid CB receptors in the amygdalar cholecystokinin glutamatergic afferents to nucleus accumbens modulate depressive-like behavior.

Nat Med 2019 02 14;25(2):337-349. Epub 2019 Jan 14.

Center for Neuroscience and Department of Neurology of Second Affiliated Hospital, NHC and CAMS Key Laboratory of Medical Neurobiology, Joint Institute for Genetics and Genome Medicine between Zhejiang University and University of Toronto, Zhejiang University School of Medicine, Hangzhou, China.

Major depressive disorder is a devastating psychiatric disease that afflicts up to 17% of the world's population. Postmortem brain analyses and imaging studies of patients with depression have implicated basal lateral amygdala (BLA) dysfunction in the pathophysiology of depression. However, the circuit and molecular mechanisms through which BLA neurons modulate depressive behavior are largely uncharacterized. Here, in mice, we identified that BLA cholecystokinin (CCK) glutamatergic neurons mediated negative reinforcement via D2 medium spiny neurons (MSNs) in the nucleus accumbens (NAc) and that chronic social defeat selectively potentiated excitatory transmission of the CCK-D2 circuit in susceptible mice via reduction of presynaptic cannabinoid type-1 receptor (CBR). Knockdown of CBR in the CCK-D2 circuit elevated synaptic activity and promoted stress susceptibility. Notably, selective inhibition of the CCK-D2 circuit or administration of synthetic cannabinoids in the NAc was sufficient to produce antidepressant-like effects. Overall, our studies reveal the circuit and molecular mechanisms of depression.
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http://dx.doi.org/10.1038/s41591-018-0299-9DOI Listing
February 2019

Versatile Types of Organic/Inorganic Nanohybrids: From Strategic Design to Biomedical Applications.

Chem Rev 2019 02 28;119(3):1666-1762. Epub 2018 Dec 28.

State Key Laboratory of Chemical Resource Engineering, Beijing Laboratory of Biomedical Materials, Key Laboratory of Carbon Fiber and Functional Polymers (Beijing University of Chemical Technology), Ministry of Education , Beijing University of Chemical Technology , Beijing , 100029 , China.

Organic/inorganic nanohybrids have attracted widespread interests due to their favorable properties and promising applications in biomedical areas. Great efforts have been made to design and fabricate versatile nanohybrids. Among different organic components, diverse polymers offer unique avenues for multifunctional systems with collective properties. This review focuses on the design, properties, and biomedical applications of organic/inorganic nanohybrids fabricated from inorganic nanoparticles and polymers. We begin with a brief introduction to a variety of strategies for the fabrication of functional organic/inorganic nanohybrids. Then the properties and functions of nanohybrids are discussed, including properties from organic and inorganic parts, synergistic properties, morphology-dependent properties, and self-assembly of nanohybrids. After that, current situations of nanohybrids applied for imaging, therapy, and imaging-guided therapy are demonstrated. Finally, we discuss the prospect of organic/inorganic nanohybrids and highlight the challenges and opportunities for the future investigations.
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http://dx.doi.org/10.1021/acs.chemrev.8b00401DOI Listing
February 2019

Predictive value of combining the level of lipoprotein-associated phospholipase A2 and antithrombin III for acute coronary syndrome risk.

Biomed Rep 2018 Dec 19;9(6):517-522. Epub 2018 Oct 19.

Department of Cardiology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu 221002, P.R. China.

The aim of the present study was to observe the role of lipoprotein-associated phospholipase A2 (Lp-PLA2) and antithrombin III (AT-III) in patients with acute coronary syndrome (ACS), and the combination of Global Registry of Acute Coronary Events (GRACE) score to determine the value of coronary heart disease risk stratification. A total of 309 patients admitted to the Affiliated Hospital of Xuzhou Medical University were enrolled. The patients were divided into two groups: The ACS (183 cases) and control (126 cases) groups. Blood levels of Lp-PLA2 and AT-III were measured. Based on GRACE score, the patients with ACS were divided into three subgroups: Low risk (GRACE score ≤108), middle risk (GRACE score 109-140) and high risk (GRACE score >140). The levels of Lp-PLA2 and AT-III were compared among different groups, and based on Gensini score, patients with ACS were divided into four groups by quartiles. Lp-PLA2 levels in the ACS group were significantly increased compared with the control group (P<0.05), but the AT-III levels were decreased compared with the control group (P<0.05). In the ACS group, Lp-PLA2 levels increased sequentially from the low risk to high risk subgroups (all P<0.05); compared with the low risk and middle risk subgroups, the AT-III activity levels were decreased in the high risk subgroup, and the Gensini scores were increased (all P<0.05). In the ACS group, with the increase of Gensini scores, the levels of Lp-PLA2 and AT-III exhibited increasing and decreasing trends, respectively (P<0.05). The logistic regression model demonstrated that Lp-PLA2 [odds ratio (OR) =1.077; P<0.001]; and GRACE score (OR=1.026; P=0.028) were risk factors, while AT-III was a protective factor (OR=0.958; P=0.012) for ACS. Correlation analysis indicated a positive association of Lp-PLA2 level with Gensini scores (r=0.52; P<0.01) and GRACE score (r=0.48; P<0.01), and a negative association between AT-III level and Gensini scores (r=-0.25; P<0.01) and GRACE scores (r=-0.34; P<0.01). The levels of Lp-PLA2 and AT-III exhibited predictive values in patients with ACS, and are associated with the severity of coronary artery stenosis.
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http://dx.doi.org/10.3892/br.2018.1162DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6256189PMC
December 2018

Protective effect of astaxanthin against contrast-induced acute kidney injury via SIRT1-p53 pathway in rats.

Int Urol Nephrol 2019 Feb 19;51(2):351-358. Epub 2018 Nov 19.

Institute of Cardiovascular Disease Research, Xuzhou Medical University, Xuzhou, 221002, Jiangsu, China.

Purpose: The present study was designed to further investigate the protective effect of astaxanthin (AST) on contrast-induced acute kidney injury (CI-AKI) in rats and the relationship between SIRT1-p53 pathway and astaxanthin.

Methods: 40 adult male Sprague Dawley (SD) rats were randomly divided into five groups (n = 8/group): control (CON), normal rats treated with AST (AST), CM-treated (CM), CM rats treated with isoform of nitric oxide synthase (iNOS) inhibitor (iNOS + CM), and CM rats treated with AST (AST + CM). Serum creatinine (Scr) and blood urea nitrogen (BUN) values were measured at 72 h following the procedure. Hematoxylin and eosin (H-E) staining was used to observe the pathologic changes of kidney. Tunel staining was used to test apoptosis of kidney tubules. Oxidative stress, SIRT1 activity, nitric oxide (NO), and 3-nitrotyrosine (3-NT) content were individually measured with the commercial available kits.

Results: Compared with the CON group, Scr and BUN levels significantly increased in the CM group (P < 0.05), and the values in two pre-treatment groups (iNOS + CM and AST + CM) had significantly decreased (P < 0.05). H-E and Tunel staining had shown that renal tubular injury was severe in CM group. The renal injury score and apoptosis index in the two pre-treatment groups also decreased (P < 0.05). The present study showed that in CM group the levels of oxidative stress indicators significantly increased, and the activities of antioxidant stress indicators significantly decreased. These indicators in two pre-treatment groups significantly improved (P < 0.05). In the CM group the expression levels of SITR1 significantly increased, and the ac-p53/p53 significantly increased (P < 0.05). Compared with the CM group, in AST + CM group the expression levels of SIRT1 increased, the expression levels of p53 and ac-p53/p53 decreased (P < 0.05).The levels of NO and 3-NT in CM group significantly increased (P < 0.05). Compared the CM group, the levels in the two pre-treatment groups significantly decreased (P < 0.05).

Conclusions: Astaxanthin has a protective effect on CI-AKI, the mechanism may be related to the SIRT1-p53 pathway. Astaxanthin can reduce the content of NO and 3-NT in renal tissue of CI-AKI, and alleviate the renal injury induced by contrast agents.
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http://dx.doi.org/10.1007/s11255-018-2027-2DOI Listing
February 2019

EZH2 RIP-seq Identifies Tissue-specific Long Non-coding RNAs.

Curr Gene Ther 2018 ;18(5):275-285

Department of Cardiology, Central Laboratory, Renmin Hospital, Wuhan University, Wuhan 430060, China.

Background: Polycomb Repressive Complex 2 (PRC2) catalyzes histone methylation at H3 Lys27, and plays crucial roles during development and diseases in numerous systems. Its catalytic subunit EZH2 represents a key nuclear target for long non-coding RNAs (lncRNAs) that emerging to be a novel class of epigenetic regulator and participate in diverse cellular processes. LncRNAs are characterized by high tissue-specificity; however, little is known about the tissue profile of the EZH2- interacting lncRNAs.

Objective: Here we performed a global screening for EZH2-binding lncRNAs in tissues including brain, lung, heart, liver, kidney, intestine, spleen, testis, muscle and blood by combining RNA immuno- precipitation and RNA sequencing. We identified 1328 EZH2-binding lncRNAs, among which 470 were shared in at least two tissues while 858 were only detected in single tissue. An RNA motif with specific secondary structure was identified in a number of lncRNAs, albeit not in all EZH2-binding lncRNAs. The EZH2-binding lncRNAs fell into four categories including intergenic lncRNA, antisense lncRNA, intron-related lncRNA and promoter-related lncRNA, suggesting diverse regulations of both cis and trans-mechanisms. A promoter-related lncRNA Hnf1aos1 bound to EZH2 specifically in the liver, a feature same as its paired coding gene Hnf1a, further confirming the validity of our study. In addition to the well known EZH2-binding lncRNAs like Kcnq1ot1, Gas5, Meg3, Hotair and Malat1, majority of the lncRNAs were firstly reported to be associated with EZH2.

Conclusion: Our findings provide a profiling view of the EZH2-interacting lncRNAs across different tissues, and suggest critical roles of lncRNAs during cell differentiation and maturation.
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http://dx.doi.org/10.2174/1566523218666181008125010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6249712PMC
October 2019

Light-Emitting Plexciton: Exploiting Plasmon-Exciton Interaction in the Intermediate Coupling Regime.

ACS Nano 2018 Oct 20;12(10):10393-10402. Epub 2018 Sep 20.

The Institute for Advanced Studies , Wuhan University , Wuhan 430072 , China.

The interaction between plasmons in metal nanostructures and excitons in layered materials attracts recent interests due to its fascinating properties inherited from the two constituents, e.g., the high tunability on its spectral or spatial properties from the plasmonic component, and the large optical nonlinearity or light emitting properties from the excitonic counterpart. Here, we demonstrate light-emitting plexcitons from the coupling between the neutral excitons in monolayer WSe and highly confined nanocavity plasmons in the nanocube-over-mirror system. We observe, simultaneously, an anticrossing dispersion curve of the hybrid system in the dark-field scattering spectrum and a 1700 times enhancement in the photoluminescence. We attribute the large photoluminescence enhancement to the increased local density of states by both the plasmonic and excitonic constituents in the intermediate coupling regime. In addition, increasing the confinement of the hybrid systems is achieved by shrinking down the size of the hot spot within the gap between the nanocube and the metal film. Numerical calculations reproduce the experimental observations and provide the effective number of excitons taking part in the interaction. This highly compact system provides a room temperature testing platform for quantum cavity electromagnetics at the deep subwavelength scale.
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http://dx.doi.org/10.1021/acsnano.8b05880DOI Listing
October 2018

Cinobufagin Induces Apoptosis in Osteosarcoma Cells Via the Mitochondria-Mediated Apoptotic Pathway.

Cell Physiol Biochem 2018 13;46(3):1134-1147. Epub 2018 Apr 13.

Department of Orthopedics, First Hospital of Wuhan, Wuhan, China.

Background/aims: Osteosarcoma is a common primary malignant bone tumor that mainly occurs in childhood and adolescence. Despite developments in the diagnosis and treatment of osteosarcoma, the prognosis is still very poor. Cinobufagin is an active component in the anti-tumor Chinese medicine called "Chan Su", and we previously revealed that cinobufagin induced apoptosis and reduced the viability of osteosarcoma cells; however, the underlying mechanism remains to be elucidated. Herein, the present study was undertaken to illuminate the molecular mechanism of cinobufagin-induced apoptosis of osteosarcoma cell.

Methods: U2OS and 143B cells were treated with different concentrations of cinobufagin. Cell viability, colony formation ability and morphological changes were assessed by a CCK-8 assay, a clonogenic assay and light microscopy, respectively. Cell apoptosis was detected by Hoechst 33258 and Annexin V-FITC/PI staining. Reactive oxygen species (ROS) and mitochondrial membrane potential (ΔΨm) were determined by flow cytometry. Glutathione (GSH) levels were detected by a GSH and GSSG assay kit. The levels of apoptosis-related proteins were determined by western blotting, and 143B cells were introduced to establish a xenograft tumor model. The effect of cinobufagin on osteosarcoma was further investigated in vivo.

Results: Our results showed that cinobufagin significantly reduced the viability of U2OS and 143B cells in vitro in a dose-and time-dependent manner. In addition, cinobufagin-induced apoptosis in U2OS and 143B cells was concentration-dependent. Moreover, we found that cinobufagin treatment increased the level of intracellular ROS, decreased ΔΨm, reduced GSH and inhibited GSH reductase (GR). The effects of cinobufagin on cell proliferation, apoptosis, ROS generation and ΔΨm loss were dramatically reversed when the cells were pretreated with the thiol-antioxidants NAC or GSH. Moreover, cinobufagin treatment increased the expression of the pro-apoptotic protein Bax and decreased the expression of the anti-apoptitic protein Bcl-2, thus altering the ratio of Bax to Bcl-2. Furthermore, Cinobufagin treatment caused cytochrome c release from the mitochondria to cytoplasm, thus increasing the protein levels of cleaved-caspase family members to induce apoptosis. Ac-DEVD-CHO or Z-LEHD-FMK significantly reduced cinobufagin-induced apoptosis. Finally, a subcutaneous xenograft animal study verified that cinobufagin also significantly suppressed osteosarcoma growth in vivo.

Conclusions: Our present data demonstrated that cinobufagin triggered cell apoptosis in osteosarcoma cells via the intrinsic mitochondria-dependent apoptosis pathway by the accumulation of ROS and the loss of ΔΨm. In an in vivo subcutaneous xenograft model, cinobufagin exhibited excellent tumor inhibitory effects. These results suggest that cinobufagin might potentially be further developed as an anti-tumor candidate for treating osteosarcoma patients in the clinic.
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http://dx.doi.org/10.1159/000488842DOI Listing
July 2018

Mimicking plasmonic nanolaser emission by selective extraction of electromagnetic near-field from photonic microcavity.

Nanoscale 2018 Apr;10(16):7431-7439

School of Physics and Technology, Center for Nanoscience and Nanotechnology, and Key Laboratory of Artificial Micro- and Nano-structures of Ministry of Education, Wuhan University, Wuhan 430072, China.

Plasmonic nanolasers have attracted significant attention owing to their ability to generate a coherent optical field in the deep subwavelength region, and they exhibit promising applications in integrated photonics, bioimaging and sensing. However, the demonstration of lasing in individual metallic nanoparticles with 3D subwavelength confinement represents a significant challenge and is yet to be realized. Herein, we propose to mimic a plasmonic nanolaser via selective scattering off the evanescent tail of a lasing photonic nanobelt using a single silver nanorod (24 nm × 223 nm). The nanorod acts as an optical antenna that selectively extracts the near-field component along the rod axis. The light output from the silver nanorod mimics the emission of a plasmonic nanolaser in its localized near-field and polarization dependence, except for the lasing wavelength and linewidth, which are inherited from the photonic laser. The realization of localized coherent light sources provides promising nanoscale lighting that shows potential in background-suppressed illumination, biosensing and imaging.
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http://dx.doi.org/10.1039/c8nr00102bDOI Listing
April 2018

Astaxanthin attenuates contrast agent-induced acute kidney injury in vitro and in vivo via the regulation of SIRT1/FOXO3a expression.

Int Urol Nephrol 2018 Jun 24;50(6):1171-1180. Epub 2018 Jan 24.

Department of Cardiology, The Affiliated Hospital of Xuzhou Medical University, No. 99 Huaihai West Road, Xuzhou, 221002, Jiangsu, China.

Purpose: The study was processed to investigate the effect of astaxanthin (AST; 3,3-dihydroxybeta, beta-carotene-4,4-dione) on the acute kidney injury induced by iohexol and the relationship with SIRT1/FOXO3a signal pathway.

Methods: Thirty male Sprague Dawley rats were randomly divided into five groups as follows: control group (CON; olive oil only), contrast media group, astaxanthin control group (100 mg/kg), low astaxanthin dose group (LAG, 50 mg/kg) and high astaxanthin dose group (HAG, 100 mg/kg). As followed, serum creatinine (SCr), blood urea nitrogen (BUN), the oxidative stress markers and apoptosis-related proteins were detected. Human proximal tubular epithelial cells (HK-2) were cultured in DMEM/F12 medium in vitro and then randomly divided into appropriate experimental groups: normal group (N), dimethyl sulfoxide (DMSO), iohexol group (I), iohexol + (1.0, 10.0 μmol/L) astaxanthin group (I + LAST; I + HAST), iohexol + SIRT1 inhibitors (nicotinamide) group (NA) and iohexol + si-RNA FOXO3a group (si-RNA FOXO3a); when cultured for 24 h, cell proliferation ability was tested by cell counting kit (CCK-8), reactive oxygen species (ROS) were detected by flow cytometry and the expression of SIRT1 and FOXO3a were observed using western blot.

Results: At the end of the experiment, the levels of SCr, BUN and malondialdehyde (MDA) were all increased in the CM group. The LAG and HAG reduce superoxide anion (SOD) activity, catalase (CAT) activity, glutathione peroxidase (GPx) activity and glutathione (GSH) content, as well as SCr and BUN level. Moreover, apoptosis-associated proteins, caspase 3 p17, bax and bcl-2 were upregulated. In HK-2 cells, after adding iohexol, proliferation and intracellular ROS levels were significantly increased. Using astaxanthin in advance after the intervention, the result is opposite. SIRTl inhibitors NA can reduce the expression of SIRTl and decrease the expression of FOXO3a protein. Si-RNA FOXO3a reduces the expression of FOXO3a but had no significant effect on the expression of SIRT1.

Conclusions: Our study demonstrates that the intervention of astaxanthin could attenuate the oxidative stress and apoptosis in contrast-induced acute kidney injury (CI-AKI), and the SIRT1/FOXO3a pathway participates in the contrast-induced apoptosis of HK-2 cells. Finally, astaxanthin reduces CI-AKI by suppression of apoptosis, which may be through inhibition of SIRT1/FOXO3a pathways.
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http://dx.doi.org/10.1007/s11255-018-1788-yDOI Listing
June 2018

Baicalein inhibits progression of osteosarcoma cells through inactivation of the Wnt/β-catenin signaling pathway.

Oncotarget 2017 Oct 18;8(49):86098-86116. Epub 2017 Sep 18.

Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, P. R. China.

Osteosarcoma is a very common type of malignant bone tumor in children and young adults and aberrant activation of Wnt/β-catenin signaling pathway has been discovered in osteosarcoma. The traditional Chinese medicine baicalein was proved to have anti-proliferative and anti-metastatic properties in osteosarcoma, but the mechanism remained poorly understood. In the present study, we assessed the effects of baicalein on osteosarcoma and detected the potential molecular mechanism. We found that baicalein significantly suppressed the proliferation of osteosarcoma cells in a concentration- and time-dependent manner. In additional, baicalein could induce apoptosis and cell cycle arrest and reduce cell motility. Moreover, the level of β-catenin and its target genes, including c-myc, cyclinD1, and survivin significantly decreased in baicalein-treated osteosarcoma cells, whereas exogenous expression of β-catenin could reverse the anti-proliferative and anti-metastatic effects of baicalein. Subsequently, we established a 143B xenograft tumor model and found that baicalein treatment significantly inhibited tumor growth accompanied with inhibiting Wnt/β-catenin pathway. Thus, these findings suggest that baicalein may be a potentially effective Chinese herbal medicine for therapeutics of osteosarcoma and Wnt/β-catenin signaling pathway may serve as an efficient molecular marker or predictive target for osteosarcoma.
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http://dx.doi.org/10.18632/oncotarget.20987DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5689670PMC
October 2017
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