Publications by authors named "Di Wang"

1,294 Publications

  • Page 1 of 1

Antarctic krill-derived peptides with consecutive Glu residues enhanced iron binding, solubility, and absorption.

Food Funct 2021 Aug 3. Epub 2021 Aug 3.

School of Food Science and Technology, National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, P. R. China. and Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian 116034, P. R. China.

Three peptides containing three glutamic acid (Glu) residues at different positions derived from Antarctic krill were obtained to investigate their iron-binding properties, digestive stability, and effectiveness on enhancing iron solubility and absorption. Results indicated that Fe2+ bound to the carbonyl, carboxyl, or hydroxyl groups of DELEDSLER, EEEFDATR, and DTDSEEEIR at stoichiometric ratios of 0.453, 0.466, and 0.490, respectively. DTDSEEEIR with three consecutive Glu in the middle of the sequence possessed higher iron-binding ability and iron release potential than EEEFDATR with three consecutive Glu in the N-terminal, and DELEDSLER with three discontinuous Glu showed the lowest values. Although EEEFDATR showed remarkably lower digestion stability than DTDSEEEIR, the effect of EEEFDATR-iron on iron solubility and absorption was comparable to that of DTDSEEEIR-iron, but better than that of DELEDSLER-iron and FeSO4. Thus, peptides with consecutive Glu have the potential as an effective iron carrier to improve iron absorption.
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http://dx.doi.org/10.1039/d1fo01405fDOI Listing
August 2021

SUMOylation of IGF2BP2 promotes vasculogenic mimicry of glioma via regulating OIP5-AS1/miR-495-3p axis.

Int J Biol Sci 2021 13;17(11):2912-2930. Epub 2021 Jul 13.

Department of Neurosurgery, Shengjing Hospital of China Medical University, Shenyang, China.

Glioma is the most common primary malignant tumor of human central nervous system, and its rich vascular characteristics make anti-angiogenic therapy become a therapeutic hotspot. However, the existence of glioma VM makes the anti-angiogenic therapy ineffective. SUMOylation is a post-translational modification that affects cell tumorigenicity by regulating the expression and activity of substrate proteins. The binding and modification of IGF2BP2 and SUMO1 were identified using Ni-NTA agarose bead pull-down assays, CO-IP and western blot; and in vitro SUMOylation assays combined with immunoprecipitation and immunofluorescence staining were performed to explore the detail affects and regulations of the SUMOylation on IGF2BP2. RT-PCR and western blot were used to detect the expression levels of IGF2BP2, OIP5-AS1, and miR-495-3p in glioma tissues and cell lines. CCK-8 assays, cell transwell assays, and three-dimensional cell culture methods were used for evaluating the function of IGF2BP2, OIP5-AS1, miR-495-3p, HIF1A and MMP14 in biological behaviors of glioma cells. Meantime, RIP and luciferase reporter assays were used for inquiring into the interactions among IGF2BP2, OIP5-AS1, miR-495-3p, HIF1A and MMP14. Eventually, the tumor xenografts in nude mice further as certained the effects of IGF2BP2 SUMOylation on glioma cells. This study proved that IGF2BP2 mainly binds to SUMO1 and was SUMOylated at the lysine residues K497, K505 and K509 sites, which can be reduced by SENP1. SUMOylation increased IGF2BP2 protein expression and blocked its degradation through ubiquitin-proteasome pathway, thereby increasing its stability. The expressions of IGF2BP2 and OIP5-AS1 were up-regulated and the expression of miR-495-3p was down-regulated in both glioma tissues and cells. IGF2BP2 enhances the stability of OIP5-AS1, thereby increasing the binding of OIP5-AS1 to miR-495-3p, weakening the binding of miR-495-3p to the 3'UTR of HIF1A and MMP14 mRNA, and ultimately promoting the formation of VM in glioma. This study first revealed that SUMOylation of IGF2BP2 regulated OIP5-AS1/miR-495-3p axis to promote VM formation in glioma cells and xenografts growth in nude mice, providing a new idea for molecular targeted therapy of glioma.
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http://dx.doi.org/10.7150/ijbs.58035DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8326132PMC
July 2021

Gut Microbiota Composition is Associated with Responses to Peanut Intervention in Multiple Parameters Among Adults with Metabolic Syndrome Risk.

Mol Nutr Food Res 2021 Jul 30:e2001051. Epub 2021 Jul 30.

CAS Key Laboratory of Nutrition, Metabolism and Food Safety, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, 200031, China.

Scope: Peanuts are widely consumed as a meal ingredient and a snack, and are commonly considered as a healthy food based on their nutrient profile. Peanut consumption has been associated with a lower risk of metabolic syndrome (MetS) in epidemiological studies. In this study, we aimed to investigate whether consuming peanuts affects the gut microbiota in adults with risk of metabolic syndrome and whether the intervention effect of peanuts is associated with gut microbiota composition.

Methods And Results: We analyzed the gut microbiota of subjects from a 12-week randomized clinical trial comparing consumption of either peanuts or isocaloric carbohydrate bars. We observed that there was high inter-individual variability on multiple clinical and anthropometrical parameters in response to peanut consumption. Meanwhile, the gut microbiota composition was also highly person-specific and had minor changes when compared laterally or longitudinally. We employed a machine-learning algorithm and established prediction models using the microbiome data and the responsiveness data of different parameters in subjects with peanut intervention. As a result, we found that the improvement of MetS risk and numerous parameters including diastolic blood pressure, body weight, waist circumference, and fasting blood glucose level could be predicted for responsiveness with high accuracy that had a value of area under curve over 0.70 by receiver operating characteristic analysis.

Conclusion: Together, our findings suggest that individual gut microbiota configuration may modulate host metabolism and alter an individual's response to peanut intervention, thus highlighting the importance of personalized nutrition. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/mnfr.202001051DOI Listing
July 2021

NIR-triggered photocatalytic and photothermal performance for sterilization based on copper sulfide nanoparticles anchored on TiCT MXene.

J Colloid Interface Sci 2021 Jul 16;604:810-822. Epub 2021 Jul 16.

School of Materials Science and Engineering, Ocean University of China, Qingdao 266100, China. Electronic address:

Harmful bacterial flourish with the increase in environmental pollution and pose a great threat to human health. Thus, developing new and efficient antibacterial materials is imperative to reduce the pollution caused by traditional sterilization materials and improve sterilization efficiency. In this study, a new photocatalytic antibacterial material was developed to achieve an efficient antibacterial effect. [email protected] composites were synthesized by simple hydrothermal method, by which copper sulfide (CuS) nanoparticles were anchored on the surface of TiCT to sharply improve the photocatalytic its antibacterial ability. [email protected] exhibits excellent antibacterial activity against Escherichia coli and Staphylococcus aureus with bactericidal rates of 99.6% and 99.1%, respectively. Photoluminescence spectroscopy (PL), decay time PL, photocurrent test, electrochemical impedance spectroscopy and finite element method showed that the formation of [email protected] heterojunction promoted the separation of electrons and holes, improved the electron transport efficiency, and elevated the generation of reactive oxygen species. Moreover, [email protected] has a stronger photothermal effect and causes more heat release than CuS to improve antibacterial performance. The [email protected] heterojunction has a broad application prospect in the disinfection and antibacterial fields.
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http://dx.doi.org/10.1016/j.jcis.2021.07.048DOI Listing
July 2021

Physical Mechanism of Photoinduced Charge Transfer in One- and Two-Photon Absorption in D-D-π-A Systems.

Materials (Basel) 2021 Jul 14;14(14). Epub 2021 Jul 14.

College of Physics, Liaoning University, Shenyang 110036, China.

The photoinduced charge transfer process of a D-π-A molecule (W1) and three D-D-π-A molecules (WS5-WS7) with triphenylamine as a donor was studied theoretically. D-D-π-A molecules are formed by inserting donors between the triphenylamine and π-linker (π-bridge) on the base of the W1 molecule. The results showed that donor insertion resulted in a red shift in the absorption spectrum, and the absorption intensity increased to a certain extent. A visualization method was used to observe the charge transfer of the four molecules in the process of one- and two-photon absorption (TPA). The local excitation enhanced charge transfer excitation in the TPA process was analyzed and discussed, and the insertion of the thiazolo[5,4-d]thiazole donor showed the largest TPA cross-section. This work contributed to the profound understanding of D-D-π-A molecules and the design of large cross-section TPA molecules.
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http://dx.doi.org/10.3390/ma14143925DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8306063PMC
July 2021

Design of Double-Layer Electrically Extremely Small-Size Displacement Sensor.

Sensors (Basel) 2021 Jul 20;21(14). Epub 2021 Jul 20.

Department of Electronics, Peking University, Beijing 100871, China.

In this paper, a displacement sensor with an electrically extremely small size and high sensitivity is proposed based on an elaborately designed metamaterial element, i.e., coupled split-ring resonators (SRRs). The sensor consists of a feeding structure with a rectangular opening loop and a sensing structure with double-layer coupled SRRs. The movable double-layer structures can be used to measure the relative displacement. The size of microwave displacement sensors can be significantly reduced due to the compact feeding and sensing structures. By adjusting the position of the split gap within the resonator, the detection directions of the displacement sensing can be further expanded accordingly (along with the - or -axis) without increasing its physical size. Compared with previous works, the extremely compact size of 0.05 × 0.05 ( denotes the free-space wavelength), a high sensitivity, and a high quality factor (-factor) can be achieved by the proposed sensor. From the perspective of the advantages above, the proposed sensor holds promise for being applied in many high-precision industrial measurement scenarios.
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http://dx.doi.org/10.3390/s21144923DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8309817PMC
July 2021

Enhancing the graphene photocurrent using surface plasmons and a p-n junction.

Light Sci Appl 2020 Jul 20;9(1):126. Epub 2020 Jul 20.

School of Electrical and Computer Engineering, Purdue University, West Lafayette, IN, 47907, USA.

The recently proposed concept of graphene photodetectors offers remarkable properties such as unprecedented compactness, ultrabroadband detection, and an ultrafast response speed. However, owing to the low optical absorption of pristine monolayer graphene, the intrinsically low responsivity of graphene photodetectors significantly hinders the development of practical devices. To address this issue, numerous efforts have thus far been made to enhance the light-graphene interaction using plasmonic structures. These approaches, however, can be significantly advanced by leveraging the other critical aspect of graphene photoresponsivity enhancement-electrical junction control. It has been reported that the dominant photocarrier generation mechanism in graphene is the photothermoelectric (PTE) effect. Thus, the two energy conversion mechanisms involved in the graphene photodetection process are light-to-heat and heat-to-electricity conversions. In this work, we propose a meticulously designed device architecture to simultaneously enhance the two conversion efficiencies. Specifically, a gap plasmon structure is used to absorb a major portion of the incident light to induce localized heating, and a pair of split gates is used to produce a p-n junction in graphene to augment the PTE current generation. The gap plasmon structure and the split gates are designed to share common key components so that the proposed device architecture concurrently realizes both optical and electrical enhancements. We experimentally demonstrate the dominance of the PTE effect in graphene photocurrent generation and observe a 25-fold increase in the generated photocurrent compared to the un-enhanced cases. While further photocurrent enhancement can be achieved by applying a DC bias, the proposed device concept shows vast potential for practical applications.
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http://dx.doi.org/10.1038/s41377-020-00344-1DOI Listing
July 2020

Identification of Proteomic Signatures in Chronic Obstructive Pulmonary Disease Emphysematous Phenotype.

Front Mol Biosci 2021 1;8:650604. Epub 2021 Jul 1.

Department of Pulmonary and Critical Care Medicine, Shengjing Hospital of China Medical University, Shenyang, China.

Chronic obstructive pulmonary disease (COPD) is a highly heterogeneous disease. Emphysematous phenotype is the most common and critical phenotype, which is characterized by progressive lung destruction and poor prognosis. However, the underlying mechanism of this structural damage has not been completely elucidated. A total of 12 patients with COPD emphysematous phenotype (COPD-E) and nine patients with COPD non-emphysematous phenotype (COPD-NE) were enrolled to determine differences in differential abundant protein (DAP) expression between both groups. Quantitative tandem mass tag-based proteomics was performed on lung tissue samples of all patients. A total of 29 and 15 lung tissue samples from patients in COPD-E and COPD-NE groups, respectively, were used as the validation cohort to verify the proteomic analysis results using western blotting. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were conducted for DAPs. A total of 4,343 proteins were identified, of which 25 were upregulated and 11 were downregulated in the COPD-E group. GO and KEGG analyses showed that wound repair and retinol metabolism-related pathways play an essential role in the molecular mechanism of COPD emphysematous phenotype. Three proteins, namely, KRT17, DHRS9, and FMO3, were selected for validation. While KRT17 and DHRS9 were highly expressed in the lung tissue samples of the COPD-E group, FMO3 expression was not significantly different between both groups. In conclusion, KRT17 and DHRS9 are highly expressed in the lung tissue of patients with COPD emphysematous phenotype. Therefore, these proteins might involve in wound healing and retinol metabolism in patients with emphysematous phenotype and can be used as phenotype-specific markers.
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http://dx.doi.org/10.3389/fmolb.2021.650604DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8280333PMC
July 2021

Combined anti-hepatocellular carcinoma therapy inhibit drug-resistance and metastasis via targeting "substance P-hepatic stellate cells-hepatocellular carcinoma" axis.

Biomaterials 2021 Jul 9;276:121003. Epub 2021 Jul 9.

School of Bioscience and Technology, Weifang Medical University, PR China. Electronic address:

Peripheral nerves have emerged as the important components in tumor microenvironment (TME), which could activate hepatic stellate cells (HSCs) by secreting substance P (SP), leading to hepatocellular carcinoma (HCC) invasion and metastasis. Herein, we proposed a novel anti-HCC concept of blocking "SP-HSCs-HCC" axis for omnidirectional inhibition of HCC development. To pursue this aim, the novel CAP/GA-sHA-DOX NPs were developed for targeted co-delivery of capsaicin (CAP) and doxorubicin (DOX) using glycyrrhetinic acid (GA) modified sulfated-HA (sHA) as nanocarriers. Among that, CAP could inhibit the activation of HSCs as an inhibitor of SP. Notably, to real mimic "SP-HSCs-HCC" axis for in vitro and in vivo evaluation, both "SP + LX-2+BEL-7402" co-cultured cell model and "SP + m-HSC + H22" co-implantation mice model were attempted for the first time. Furthermore, in vivo anti-HCC effects were performed in three different tumor-bearing models: subcutaneous implantation of H22 or "SP + m-HSC + H22", intravenous injection of H22 for lung metastasis, and orthotopic implantation of H22 for primary HCC. Our results showed that CAP/GA-sHA-DOX NPs could be efficiently taken up by tumor cells and activated HSCs (aHSCs) simultaneously, and effectively inhibit tumor drug-resistance and migration by blocking SP-induced HSCs activation. In addition, CAP/GA-sHA-DOX NPs exhibited low ECM deposition, less tumor angiogenesis, and superior in vivo anti-HCC effects. The anti-HCC mechanisms revealed that CAP/GA-sHA-DOX NPs could down-regulate the expression level of Vimentin and P-gp, reverse epithelial-mesenchymal transition (EMT) of tumor cells. In brief, the nano-sized combination therapy based on GA-sHA-DOX polymers could effectively inhibit drug-resistance and metastasis of HCC by blocking "SP-HSCs-HCC" axis, which provides a promising approach for cancer therapy.
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http://dx.doi.org/10.1016/j.biomaterials.2021.121003DOI Listing
July 2021

Phase-Selective Synthesis of Ultrathin FeTe Nanoplates by Controllable Fe/Te Atom Ratio in the Growth Atmosphere.

Small 2021 Jul 16:e2101616. Epub 2021 Jul 16.

Hunan Key Laboratory of Two-Dimensional Materials, State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, China.

Phase controllable synthesis of 2D materials is of significance for tuning related electrical, optical, and magnetic properties. Herein, the phase-controllable synthesis of tetragonal and hexagonal FeTe nanoplates has been realized by a rational control of the Fe/Te ratio in a chemical vapor deposition system. Using density functional theory calculations, it has been revealed that with the change of the Fe/Te ratio, the formation energy of active clusters changes, causing the phase-controllable synthesis of FeTe nanoplates. The thickness of the obtained FeTe nanoplates can be tuned down to the 2D limit (2.8 nm for tetragonal and 1.4 nm for hexagonal FeTe). X-ray diffraction pattern, transmission electron microscopy, and high resolution scanning transmission electron microscope analyses exhibit the high crystallinity of the as-grown FeTe nanoplates. The two kinds of FeTe nanoflakes show metallic behavior and good electrical conductivity, featuring 8.44 × 10 S m for 9.8 nm-thick tetragonal FeTe and 5.45 × 10 S m for 7.6 nm-thick hexagonal FeTe. The study provides an efficient and convenient route for tailoring the phases of FeTe nanoplates, which benefits to study phase-sensitive properties, and may pave the way for the synthesis of other multiphase 2D nanosheets with controllable phases.
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http://dx.doi.org/10.1002/smll.202101616DOI Listing
July 2021

Continuous optical zoom microscopy imaging system based on liquid lenses.

Opt Express 2021 Jun;29(13):20322-20335

In this paper, a continuous optical zoom microscopy imaging system based on liquid lenses is proposed. Compared with traditional microscopes, which have discrete magnification, requiring manual conversion of the objective lens to change the magnification, the proposed microscope can continuously change the magnification of the targets in real-time. An adaptive zoom microscope, a liquid lens driving board, a microscope bracket, an adjustable three-dimensional stage and a light source are stacked to form the main framework of the continuous optical zoom microscopy imaging system. The adaptive zoom microscope which is composed of four electrowetting liquid lenses and six glass lenses form the main imaging element of the microscope. By changing the driving voltage which is applied to the four liquid lenses, the focal length of the liquid lenses can be modulated to achieve continuous zooming. By contrast, in traditional microscopes, the zooming process can only be achieved by rotating the eyepieces at different magnifications. At a fixed working distance, the magnification of the proposed microscope can change continuously from ∼9.6× to ∼22.2× with a response time of ∼50ms. Moreover, an axial depth scanning of ∼1000µm can be achieved without any mechanical movement. Our experiments proved that the microscope has stable performance and high consistency during zooming. Therefore, the proposed microscope has obvious advantages over the traditional microscopes in observing dynamic samples with different magnifications and can be commercialized for further expanding the applications in biochemical and pathological analysis.
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http://dx.doi.org/10.1364/OE.432290DOI Listing
June 2021

GABAergic Neurons in the Dorsal-Intermediate Lateral Septum Regulate Sleep-Wakefulness and Anesthesia in Mice.

Anesthesiology 2021 Jul 13. Epub 2021 Jul 13.

Background: The γ-aminobutyric acid-mediated (GABAergic) inhibitory system in the brain is critical for regulation of sleep-wake and general anesthesia. The lateral septum contains mainly GABAergic neurons, being cytoarchitectonically divided into the dorsal, intermediate, and ventral parts. This study hypothesized that GABAergic neurons of the lateral septum participate in the control of wakefulness and promote recovery from anesthesia.

Methods: By employing fiber photometry, chemogenetic and optogenetic neuronal manipulations, anterograde tracing, in vivo electrophysiology, and electroencephalogram/electromyography recordings in adult male mice, the authors measured the role of lateral septum GABAergic neurons to the control of sleep-wake transition and anesthesia emergence and the corresponding neuron circuits in arousal and emergence control.

Results: The GABAergic neurons of the lateral septum exhibited high activities during the awake state by in vivo fiber photometry recordings (awake vs. non-rapid eye movement sleep: 3.3 ± 1.4% vs. -1.3 ± 1.2%, P < 0.001, n = 7 mice/group; awake vs. anesthesia: 2.6 ± 1.2% vs. -1.3 ± 0.8%, P < 0.001, n = 7 mice/group). Using chemogenetic stimulation of lateral septum GABAergic neurons resulted in a 100.5% increase in wakefulness and a 51.2% reduction in non-rapid eye movement sleep. Optogenetic activation of these GABAergic neurons promoted wakefulness from sleep (median [25th, 75th percentiles]: 153.0 [115.9, 179.7] s to 4.0 [3.4, 4.6] s, P = 0.009, n = 5 mice/group) and accelerated emergence from isoflurane anesthesia (514.4 ± 122.2 s vs. 226.5 ± 53.3 s, P < 0.001, n = 8 mice/group). Furthermore, the authors demonstrated that the lateral septum GABAergic neurons send 70.7% (228 of 323 cells) of monosynaptic projections to the ventral tegmental area GABAergic neurons, preferentially inhibiting their activities and thus regulating wakefulness and isoflurane anesthesia depth.

Conclusions: The results uncover a fundamental role of the lateral septum GABAergic neurons and their circuit in maintaining awake state and promoting general anesthesia emergence time.

Editor’s Perspective:
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http://dx.doi.org/10.1097/ALN.0000000000003868DOI Listing
July 2021

Sensitive and rapid on-site detection of SARS-CoV-2 using a gold nanoparticle-based high-throughput platform coupled with CRISPR/Cas12-assisted RT-LAMP.

Sens Actuators B Chem 2021 Oct 6;345:130411. Epub 2021 Jul 6.

School of Biomedical Engineering, Sun Yat-sen University, Shenzhen, 518107, China.

The outbreak of corona virus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has led to a global pandemic. The high infectivity of SARS-CoV-2 highlights the need for sensitive, rapid and on-site diagnostic assays of SARS-CoV-2 with high-throughput testing capability for large-scale population screening. The current detection methods in clinical application need to operate in centralized labs. Though some on-site detection methods have been developed, few tests could be performed for high-throughput analysis. We here developed a gold nanoparticle-based visual assay that combines with CRISPR/Cas12a-assisted RT-LAMP, which is called Cas12a-assisted RT-LAMP/AuNP (CLAP) assay for rapid and sensitive detection of SARS-CoV-2. In optimal condition, we could detect down to 4 copies/μL of SARS-CoV-2 RNA in 40 min. by naked eye. The sequence-specific recognition character of CRISPR/Cas12a enables CLAP a superior specificity. More importantly, the CLAP is easy for operation that can be extended to high-throughput test by using a common microplate reader. The CLAP assay holds a great potential to be applied in airports, railway stations, or low-resource settings for screening of suspected people. To the best of our knowledge, this is the first AuNP-based colorimetric assay coupled with Cas12 and RT-LAMP for on-site diagnosis of COVID-19. We expect CLAP assay will improve the current COVID-19 screening efforts, and make contribution for control and mitigation of the pandemic.
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http://dx.doi.org/10.1016/j.snb.2021.130411DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8257267PMC
October 2021

Aspirin ameliorates pulmonary vascular remodeling in pulmonary hypertension by dampening endothelial-to-mesenchymal transition.

Eur J Pharmacol 2021 Jul 8;908:174307. Epub 2021 Jul 8.

Department of Pharmacology, Xiangya School of Pharmaceutical Sciences, Central South University, Changsha, Hunan, 410078, China; Hunan Provincial Key Laboratory of Cardiovascular Research, Central South University, Changsha, Hunan, 410078, China. Electronic address:

Pulmonary vascular remodeling (PVR) is the pathological basis of pulmonary hypertension (PH). Incomplete understanding of PVR etiology has hindered drug development for this devastating disease, which exhibits poor prognosis despite the currently available therapies. Endothelial-to-mesenchymal transition (EndMT), a process of cell transdifferentiation, has been recently implicated in cardiovascular diseases, including PH. But the questions of how EndMT occurs and how to pharmacologically target EndMT in vivo have yet to be further answered. Herein, by performing hematoxylin-eosin and immunofluorescence staining, transmission electron microscopy and Western blotting, we found that EndMT plays a key role in the pathogenesis of PH, and importantly that aspirin, a FDA-approved widely used drug, was capable of ameliorating PVR in a preclinical rat model of hypoxia-induced PH. Moreover, aspirin exerted its inhibitory effects on EndMT in vitro and in vivo by suppressing HIF-1α/TGF-β1/Smads/Snail signaling pathway. Our data suggest that EndMT represents an intriguing drug target for the prevention and treatment of hypoxic PH and that aspirin may be repurposed to meet the urgent therapeutic needs of hypoxic PH patients.
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http://dx.doi.org/10.1016/j.ejphar.2021.174307DOI Listing
July 2021

High concentrations of aluminum in maternal serum and placental tissue are associated with increased risk for fetal neural tube defects.

Chemosphere 2021 Jun 29;284:131387. Epub 2021 Jun 29.

Institute of Reproductive and Child Health/Key Laboratory of Reproductive Health, National Health Commission of the People's Republic of China, Peking University, Beijing, China; Department of Epidemiology and Biostatistics, School of Public Health, Peking University, Beijing, China. Electronic address:

Aluminum (Al) is ubiquitously present in the environment, and human exposure to Al is common. Al has been reported to be involved in various human diseases and adverse pregnancy outcomes, including neural tube defects (NTDs). This study aimed to examine the association between prenatal Al exposure and the risk for NTDs using Al concentrations in maternal serum and placental tissue. The subjects were recruited from six counties/cities in the Shanxi province of northern China from 2003 to 2016. Al concentrations in both types of specimens were assessed using inductively coupled plasma-mass spectrometry. In the maternal serum cohort (200 cases and 400 controls), compared to the lowest tertile concentration of Al, the highest Al tertile was associated with 2.42-fold (95% confidence interval, 1.23-4.87) increased risk after adjustment for confounding factors. In the placental tissue cohort (408 cases and 593 controls), the highest tertile of Al also tended to be associated with an elevated risk for NTDs [adjusted odds ratio, 1.60 (0.94-2.70)]. When analyzed by NTD subtypes, the highest Al tertile was associated with an increased risk for anencephaly in both cohorts after adjustment for confounders [odds ratio, 1.97 (1.15-3.48) in the maternal serum cohort; odds ratio, 4.75 (2.01-12.00) in the placental tissue cohort]. Taken together, using concentrations of Al in maternal serum and placental tissue as exposure markers, we found that prenatal exposure to higher levels of Al is a risk factor for fetal NTDs, especially for the anencephaly subtype.
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http://dx.doi.org/10.1016/j.chemosphere.2021.131387DOI Listing
June 2021

Platelet count as an independent risk factor for acute kidney injury induced by rhabdomyolysis.

Chin Med J (Engl) 2021 Jun 24. Epub 2021 Jun 24.

Department of Intensive Care Medicine, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, 230036, China.

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http://dx.doi.org/10.1097/CM9.0000000000001651DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8318643PMC
June 2021

Real-Time and Online Lubricating Oil Condition Monitoring Enabled by Triboelectric Nanogenerator.

ACS Nano 2021 Jun 25. Epub 2021 Jun 25.

Division of Machine Elements, Luleå University of Technology, Luleå, SE-971 87 Sweden.

An intelligent monitoring lubricant is essential for the development of smart machines because unexpected and fatal failures of critical dynamic components in the machines happen every day, threatening the life and health of humans. Inspired by the triboelectric nanogenerators (TENGs) work on water, we present a feasible way to prepare a self-powered triboelectric sensor for real-time monitoring of lubricating oils the contact electrification process of oil-solid contact (O-S TENG). Typical intruding contaminants in pure base oils can be successfully monitored. The O-S TENG has very good sensitivity, which even can respectively detect at least 1 mg mL debris and 0.01 wt % water contaminants. Furthermore, the real-time monitoring of formulated engine lubricating oil in a real engine oil tank is achieved. Our results show that electron transfer is possible from an oil to solid surface during contact electrification. The electrical output characteristic depends on the screen effect from such as wear debris, deposited carbons, and age-induced organic molecules in oils. Previous work only qualitatively identified that the output ability of liquid can be improved by leaving less liquid adsorbed on the TENG surface, but the adsorption mass and adsorption speed of liquid and its consequences for the output performance were not studied. We quantitatively study the internal relationship between output ability and adsorbing behavior of lubricating oils by quartz crystal microbalance with dissipation (QCM-D) for liquid-solid contact interfaces. This study provides a real-time, online, self-powered strategy for intelligent diagnosis of lubricating oils.
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http://dx.doi.org/10.1021/acsnano.1c02980DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8320232PMC
June 2021

Anti-BCMA CAR-T Cell Therapy in Relapsed or Refractory Multiple Myeloma Patients with Impaired Renal Function.

Curr Med Sci 2021 Jun 25;41(3):474-481. Epub 2021 Jun 25.

Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.

Anti-B cell maturation antigen (BCMA) chimeric antigen receptor (CAR) T-cell therapy is effective and well-tolerated for refractory or relapsed multiple myeloma (RRMM). The purpose of the present study was to analyze efficacy in RRMM patients with renal impairment treated by anti-BCMA CAR-T cell therapy. A total of 59 RRMM patients were selected, and divided into impaired renal function (IRF) group [baseline estimated glomerular filtration rate (eGFR) < 90 mL/min/1.73 m (n=18)] and normal renal function (NRF) group (baseline eGFR ≥ 90 mL/min/1.73 m, n=41). For patients with IRF, eGFR at the 6th month post-CAR-T cells infusion was significantly higher than the baseline (P<0.05). The multivariate analysis showed that light chain type and beta-2 micro-globulin (beta-2M) were associated factors with the decrease of serum creatinine. Median progression-free survival (PFS) in the NRF group and IRF group was 266 days and 181 days respectively. Overall survival (OS) in the NRF group and IRF group was 877 days and 238 days respectively. There was no significant difference in the objective response rate (ORR) between the IRF group and the NRF group. It is suggested that CAR-T cells therapy could improve the renal function during the treatment of RRMM. The renal function could be more significantly improved in RRMM patients with light chain type than with other types.
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http://dx.doi.org/10.1007/s11596-021-2373-7DOI Listing
June 2021

Correction: Rhodium-catalysed selective C-C bond activation and borylation of cyclopropanes.

Chem Sci 2021 Mar 3;12(10):3786-3787. Epub 2021 Mar 3.

State Key Laboratory of Coordination Chemistry, Chemistry and Biomedicine Innovation Center (ChemBIC), School of Chemistry and Chemical Engineering, Nanjing University Nanjing 210093 China

[This corrects the article DOI: 10.1039/D0SC06186G.].
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http://dx.doi.org/10.1039/d1sc90035hDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8179530PMC
March 2021

Rhodium-catalysed selective C-C bond activation and borylation of cyclopropanes.

Chem Sci 2021 Jan 15;12(10):3599-3607. Epub 2021 Jan 15.

State Key Laboratory of Coordination Chemistry, Chemistry and Biomedicine Innovation Center (ChemBIC), School of Chemistry and Chemical Engineering, Nanjing University Nanjing 210093 China

Transition metal (TM)-catalysed directed hydroboration of aliphatic internal olefins which facilitates the construction of complex alkylboronates is an essential synthetic methodology. Here, an efficient method for the borylation of cyclopropanes involving TM-catalysed directed C-C activation has been developed. Upon exposure to neutral Rh(i)-catalyst systems, -Piv-substituted cyclopropylamines (CPAs) undergo proximal-selective hydroboration with HBpin to provide valuable γ-amino boronates in one step which are otherwise difficult to synthesize by known methods. The enantioenriched substrates can deliver chiral products without erosion of the enantioselectivities. Versatile synthetic utility of the obtained γ-amino boronates is also demonstrated. Experimental and computational mechanistic studies showed the preferred pathway and the origin of this selectivity. This study will enable the further use of CPAs as valuable building blocks for the tunable generation of C-heteroatom or C-C bonds through selective C-C bond activation.
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http://dx.doi.org/10.1039/d0sc06186gDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8179453PMC
January 2021

Anti-obesity effects of through the modulation of lipid metabolism ceramide in mice fed a high-fat diet.

Food Funct 2021 Aug;12(15):6725-6739

School of Life Sciences, Jilin University, Changchun 130012, China. and Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun, 130118, China.

Obesity is characterized by massive fat deposition and is related to a series of metabolic complications, such as insulin resistance (IR) and steatohepatitis. Grifola frondosa (GF) is a basidiomycete fungus and a source of various nutritional ingredients related to human health. In this study, after a systematic analysis of its nutritional ingredients, GF was administered to mice fed a high-fat diet (HFD) to investigate its effects on lipid metabolism. In HFD-fed mice, GF significantly controlled the body weight, blood glucose and related organ indices, and effectively counteracted hyperlipidemia and IR triggered by the HFD. GF administration efficiently alleviated hepatic steatosis and adipocyte hypertrophy, and regulated alanine aminotransferase and aspartate aminotransferase in the liver. An analysis of the intestinal microflora showed that GF reversed obesity-induced dysbiosis by affecting the relative abundance of certain bacteria, reducing lipopolysaccharide production and regulating the superpathway of heme biosynthesis associated with inflammation. According to the results of lipidomics, ceramide, a metabolite related to inflammation and IR, was found to be dysregulated in HFD-fed mice. However, GF regulated the ceramide levels and restored lipid metabolism via the suppression of Toll-like receptor 4/nuclear factor kappa-B signaling, which is involved in inflammation and IR. This study provides the experimental basis for the application of GF as an agent for obesity.
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http://dx.doi.org/10.1039/d1fo00666eDOI Listing
August 2021

Circular RNA circRHOBTB3 represses metastasis by regulating the HuR-mediated mRNA stability of PTBP1 in colorectal cancer.

Theranostics 2021 1;11(15):7507-7526. Epub 2021 Jun 1.

Department of Colorectal Surgery, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, 310058 Hangzhou, China.

Tumor metastasis of colorectal cancer (CRC) is the main cause of death in most patients and the major difficulty in comprehensive CRC treatment. Circular RNAs (circRNAs) affect many biological functions in solid tumors. However, their mechanisms in CRC metastasis remain unclear. RNA sequencing (RNA-seq) and quantitative real-time PCR were performed to screen differentially expressed circRNAs between CRC tissues and adjacent normal tissues. CCK-8, cell migration and wound healing assays were performed to determine the functions of circRHOBTB3 in cell proliferation and metastasis. RNA pulldown and RNA immunoprecipitation assays were performed to verify the interaction between circRHOBTB3 and the HuR (ELAVL1) protein. Further RNA-seq and rescue experiments were applied to search for the downstream target. We also conducted a mouse xenograft model to elucidate the effect of circRHOBTB3 on cancer metastasis . We identified circRHOBTB3 which is markedly downregulated in CRC tissues and cell lines. Furthermore, lower circRHOBTB3 levels were significantly associated with advanced clinical stages and greater risk of metastases. Overexpression of circRHOBTB3 suppresses tumor metastasis in CRC cells. Mechanistically, circRHOBTB3 binds to HuR, which is a ubiquitously expressed and functional RNA-binding protein (RBP) in CRC development, and promotes β-Trcp1-mediated ubiquitination of HuR. Normally, HuR binds to the 3'UTR of target mRNAs to facilitate their stabilization, whereas the interaction between circRHOBTB3 and HuR degrades HuR to reduce the expression level of the downstream target PTBP1. Furthermore, overexpressed circRHOBTB3 suppresses lung metastases , and this effect can be partly reversed by PTBP1 overexpression. In addition, the transcription of circRHOBTB3 can be improved by both FUS and ADARB2 in CRC cells. Our findings indicate that circRHOBTB3 exerts suppressive effects on CRC aggressiveness through the HuR/PTBP1 axis.
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http://dx.doi.org/10.7150/thno.59546DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8210600PMC
July 2021

Transmissive mid-infrared achromatic bifocal metalens with polarization sensitivity.

Opt Express 2021 May;29(11):17173-17182

Metasurfaces have shown great potential in versatile areas such as vortex-beam generators, metalenses, holograms and so on. However, chromatic error hinders metasurfaces, especially metalenses, from wider applications. In this paper, we demonstrate a novel design for a transmissive mid-infrared achromatic bifocal metalens with polarization sensitivity. The compensation phase is used to eliminate the chromatic aberration. Simulation results show that, over a continuous waveband from 3.9 to 4.6µm, the focal length only changes by 2.26% with an average focusing efficiency of about 18%. This work can push the practical application of mid-infrared metasurfaces.
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http://dx.doi.org/10.1364/OE.424887DOI Listing
May 2021

Successful stereoelectroencephalography-guided radiofrequency thermocoagulation in the treatment of a patient with mesial frontal region-originated mirthful gelastic seizure.

Clin Neurol Neurosurg 2021 May 27;207:106713. Epub 2021 May 27.

Department of Neurosurgery, Xuanwu Hospital, Capital Medical University, No. 45, Changchun Street, Xicheng District, Beijing 100053, China; Beijing Institute for Brain Disorders, Capital Medical University, No. 10, West Tou Tiao, Outer You'an Men, Fengtai District, Beijing 100069, China. Electronic address:

Gelastic seizures (GS), characterized by automatic bouts of unnatural stereotyped laughter and commonly recognized as the hallmark of hypothalamic hamartoma, is rarely associated with cortical epileptogenic focus. Whether there is a dissociation of the motor program of laughter and the experience of mirth and the symptomatogenic zone for ictal laughter with or without mirth are still unclear. We report a patient with drug-resistant mirthful GS who receive a comprehensive investigation of stereoelectroencephalography recording and electrocortical stimulation. Mirthful GS were confirmed to originate from the mesial region of the right superior frontal gyrus, not involving the mesial temporal lobe structures. The patient has been entirely seizure-free after stereoelectroencephalography-guided radiofrequency thermocoagulation. We conclude that the superior frontal gyrus serves as the symptomatogenic zone of ictal laughter and GS with or without mirth share a common neural network.
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http://dx.doi.org/10.1016/j.clineuro.2021.106713DOI Listing
May 2021

A comparative study of public health and social measures of COVID-19 advocated in different countries.

Authors:
Di Wang Zhifei Mao

Health Policy 2021 08 5;125(8):957-971. Epub 2021 Jun 5.

School of Media and Communication, Shenzhen University, Shenzhen 518052, China. Electronic address:

Coronavirus 2019 (COVID-19) has had a significant impact on the world. Different countries introduced various public health and social measures (PHSMs) against the coronavirus. This paper aims to (a) examine how national policies on PHSMs were framed and which PHSMs were adopted; (b) compare the similarities and differences of PHSMs advocated by different countries; and (c) examine whether these measures have changed with time. We performed a content analysis of 160 COVID-19 policy documents on the websites of the WHO and ten countries' government websites on COVID-19 between December 1, 2019 and May 31, 2020. Results showed that although the initial responses to COVID-19 in different countries varied, there was a homogenization of PHSMs over time: by May 31, 2020, almost all the countries we studied implemented the major PHSMs that the WHO recommended, except Sweden, which applied only part of the major PHSMs recommended by the WHO. The differences among countries were in the speediness, strictness and resourcefulness of the PHSMs implementation. We suggest that a timely and effective utilization of the integrated package of health measures with the support of adequate resources may help the efficient implementation of PHSMs.
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http://dx.doi.org/10.1016/j.healthpol.2021.05.016DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8178944PMC
August 2021

Ultrasmall Molybdenum Disulfide Quantum Dots Cage Alzheimer's Amyloid Beta to Restore Membrane Fluidity.

ACS Appl Mater Interfaces 2021 Jun 18;13(25):29936-29948. Epub 2021 Jun 18.

Drug Delivery, Disposition and Dynamics, Monash Institute of Pharmaceutical Sciences, Monash University, 381 Royal Parade, Parkville, Victoria 3052, Australia.

Alzheimer's disease (AD) is a major cause of dementia characterized by the overexpression of transmembrane amyloid precursor protein and its neurotoxic byproduct amyloid beta (Aβ). A small peptide of considerable hydrophobicity, Aβ is aggregation prone catalyzed by the presence of cell membranes, among other environmental factors. Accordingly, current AD mitigation strategies often aim at breaking down the Aβ-membrane communication, yet no data is available concerning the cohesive interplay of the three key entities of the cell membrane, Aβ, and its inhibitor. Using a lipophilic Laurdan dye and confocal fluorescence microscopy, we observed cell membrane perturbation and actin reorganization induced by Aβ oligomers but not by Aβ monomers or amyloid fibrils. We further revealed recovery of membrane fluidity by ultrasmall MoS quantum dots, also shown in this study as a potent inhibitor of Aβ amyloid aggregation. Using discrete molecular dynamics simulations, we uncovered the binding of MoS and Aβ monomers as mediated by hydrophilic interactions between the quantum dots and the peptide N-terminus. In contrast, Aβ oligomers and fibrils were surface-coated by the ultrasmall quantum dots in distinct testudo-like, reverse protein-corona formations to prevent their further association with the cell membrane and adverse effects downstream. This study offers a crucial new insight and a viable strategy for regulating the amyloid aggregation and membrane-axis of AD pathology with multifunctional nanomedicine.
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http://dx.doi.org/10.1021/acsami.1c06478DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8251662PMC
June 2021

Effect of perioperative flurbiprofen axetil on long-term survival of patients with esophageal carcinoma who underwent thoracoscopic esophagectomy: A retrospective study.

J Surg Oncol 2021 Jun 18. Epub 2021 Jun 18.

Department of Anesthesiology, Anhui Provincial Hospital, Hefei, Anhui, China.

Background And Objectives: Nonsteroidal anti-inflammatory drugs (NSAIDs) have an anti-inflammatory response, but it remains unclear whether the perioperative use of flurbiprofen axetil can influence postoperative tumor recurrence and survival in esophageal carcinoma. We aimed to explore the effect of perioperative intravenous flurbiprofen axetil on recurrence-free survival (RFS) and overall survival (OS) in patients with esophageal carcinoma who underwent thoracoscopic esophagectomy.

Methods: This retrospective study included patients who underwent surgery for esophageal carcinoma between December 2009 and May 2015 at the Department of Thoracic Surgery, Anhui Provincial Hospital. Patients were categorized into a non-NSAIDs group (did not receive flurbiprofen axetil), single-dose NSAIDs group (received a single dose of flurbiprofen axetil intravenously), and multiple-dose NSAIDs group (received multiple doses of flurbiprofen).

Results: A total of 847 eligible patients were enrolled. Univariable and multivariable analyses revealed that the intraoperative use of flurbiprofen was associated with long-term RFS (hazard ratio [HR]: 0.56, 95% confidence interval [CI]: 0.42-0.76, p = .001) and prolonged OS (HR: 0.49, 95% CI: 0.38-0.63, p = .001).

Conclusions: Perioperative flurbiprofen axetil therapy may be associated with prolonged RFS and OS in patients with esophageal carcinoma undergoing thoracoscopic esophagectomy.
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http://dx.doi.org/10.1002/jso.26553DOI Listing
June 2021

Pseudogene ACTBP2 increases blood-brain barrier permeability by promoting KHDRBS2 transcription through recruitment of KMT2D/WDR5 in Aβ microenvironment.

Cell Death Discov 2021 Jun 14;7(1):142. Epub 2021 Jun 14.

Department of Neurobiology, School of life Sciences, China Medical University, Shenyang, China.

The blood-brain barrier (BBB) has a vital role in maintaining the homeostasis of the central nervous system (CNS). Changes in the structure and function of BBB can accelerate Alzheimer's disease (AD) development. β-Amyloid (Aβ) deposition is the major pathological event of AD. We elucidated the function and possible molecular mechanisms of the effect of pseudogene ACTBP2 on the permeability of BBB in Aβ microenvironment. BBB model treated with Aβ for 48 h were used to simulate Aβ-mediated BBB dysfunction in AD. We proved that pseudogene ACTBP2, RNA-binding protein KHDRBS2, and transcription factor HEY2 are highly expressed in ECs that were obtained in a BBB model in vitro in Aβ microenvironment. In Aβ-incubated ECs, ACTBP2 recruits methyltransferases KMT2D and WDR5, binds to KHDRBS2 promoter, and promotes KHDRBS2 transcription. The interaction of KHDRBS2 with the 3'UTR of HEY2 mRNA increases the stability of HEY2 and promotes its expression. HEY2 increases BBB permeability in Aβ microenvironment by transcriptionally inhibiting the expression of ZO-1, occludin, and claudin-5. We confirmed that knocking down of Khdrbs2 or Hey2 increased the expression levels of ZO-1, occludin, and claudin-5 in APP/PS1 mice brain microvessels. ACTBP2/KHDRBS2/HEY2 axis has a crucial role in the regulation of BBB permeability in Aβ microenvironment, which may provide a novel target for the therapy of AD.
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http://dx.doi.org/10.1038/s41420-021-00531-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8203645PMC
June 2021

Tryptophan-kynurenine metabolism: a link between the gut and brain for depression in inflammatory bowel disease.

J Neuroinflammation 2021 Jun 14;18(1):135. Epub 2021 Jun 14.

Yueyang Hospital of Integrated Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, No.110 Ganhe Road, Shanghai, 200437, China.

Inflammatory bowel disease (IBD), which mainly includes ulcerative colitis (UC) and Crohn's disease (CD), is a group of chronic bowel diseases that are characterized by abdominal pain, diarrhea, and bloody stools. IBD is strongly associated with depression, and its patients have a higher incidence of depression than the general population. Depression also adversely affects the quality of life and disease prognosis of patients with IBD. The tryptophan-kynurenine metabolic pathway degrades more than 90% of tryptophan (TRP) throughout the body, with indoleamine 2,3-dioxygenase (IDO), the key metabolic enzyme, being activated in the inflammatory environment. A series of metabolites of the pathway are neurologically active, among which kynerunic acid (KYNA) and quinolinic acid (QUIN) are molecules of great interest in recent studies on the mechanisms of inflammation-induced depression. In this review, the relationship between depression in IBD and the tryptophan-kynurenine metabolic pathway is overviewed in the light of recent publications.
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http://dx.doi.org/10.1186/s12974-021-02175-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8204445PMC
June 2021
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