Publications by authors named "Denis Vio"

5 Publications

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A case of pericarditis caused by Mycoplasma hyorhinis in a weaned piglet.

Porcine Health Manag 2021 Apr 12;7(1):32. Epub 2021 Apr 12.

Peripheral Diagnostic Laboratory of Pordenone, Istituto Zooprofilattico Sperimentale delle Venezie, Cordenons, PN, Italy.

Background: Mycoplasma hyorhinis (M. hyorhinis) is a bacterium commonly found in the upper respiratory tract of healthy pigs and an agent of polyserositis and polyarthritis. Moreover, it can carry antibiotic resistance genes (Wu et al, Vet. Microbiol. 76: 25-30, 2000). Economic losses caused by M. hyorhinis can be reduced by antibiotic therapy, however, isolation and antimicrobic susceptibility profile are rarely performed.

Case Presentation: The present report describes a case of pericarditis caused by M. hyorhinis in a weaned piglet with respiratory symptoms and reduced growth performance. At post mortem examination, the main macroscopic finding was a severe fibrinous pericarditis and M. hyorhins was the only agent isolated from the pericardial fluid. In this strain, Minimum Inhibitory Concentration (MIC) determination revealed resistance to various antimicrobial molecules such as erythromycin, tylosin and tilmicosin.

Conclusion: This paper highlights the importance of including M. hyorhins in the differential diagnosis of polyserositis in swine. Moreover, due the possible presence of multidrug resistance, the determination of antimicrobial susceptibility pattern should be performed on a regular basis.
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http://dx.doi.org/10.1186/s40813-021-00211-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8040207PMC
April 2021

Environmental Exposure of Wild Carnivores to Zoonotic Pathogens: Infection in the First Free Living Wolf ( Linnaeus, 1758) Found Dead in the Friuli Venezia Giulia Region.

Int J Environ Res Public Health 2021 03 3;18(5). Epub 2021 Mar 3.

Istituto Zooprofilattico Sperimentale delle Venezie, Viale dell'Università, 35020 Legnaro, Italy.

Leptospirosis is a worldwide-spread zoonosis causing disease and death in dogs and in humans. A Leptospiral infection has been recorded in several wild carnivore species in Europe, but tissue pathological changes were not commonly described. The Grey wolf () has been expanding its distribution range in north-eastern Italy during the last decade. A young wolf, representing the first individual handled in the region, was found road-killed and then submitted to necropsy. Pathological changes included erosive lesions of gingival mucosa, mild liver enlargement, and multifocal degenerative-necrotic areas along with hyperemic reactive lesions; multifocal interstitial nephritis and multifocal lung hemorrhages were observed. A Polymerase Chain Reaction (PCR) able to detect pathogenic species of performed on a kidney sample was positive. Serological reactions for serogroup Gryppotyphosa (1:6400), Pomona (1:800), and Icterohaemorrhagiae (1:200) were evidenced by MAT. Genotyping by Multilocus Sequence Typing (MLST) performed on detected characterized it as belonging to Sequence Type (ST) 117, which refers to , serogroup Pomona, serovar Mozdok. Regardless of the role of infection as an eventual predisposing factor to the road killing of this wolf, to the best of the authors' knowledge, this is the first report of -induced pathology in a wolf in Europe. Surveys on infection in free-ranging wildlife species should be pursued in order to achieve further epidemiological knowledge on the circulation of the strain.
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http://dx.doi.org/10.3390/ijerph18052512DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7967383PMC
March 2021

Business intelligence tools to optimize the appropriateness of the diagnostic process for clinical and epidemiologic purposes in a multicenter veterinary pathology service.

J Vet Diagn Invest 2021 May 26;33(3):439-447. Epub 2021 Mar 26.

Istituto Zooprofilattico Sperimentale delle Venezie, Legnaro, Italy.

Laboratory tests provide essential support to the veterinary practitioner, and their use has grown exponentially. This growth is the result of several factors, such as the eradication of historical diseases, the occurrence of multifactorial diseases, and the obligation to control endemic and epidemic diseases. However, the introduction of novel techniques is counterbalanced by economic constraints, and the establishment of evidence- and consensus-based guidelines is essential to support the pathologist. Therefore, we developed standardized protocols, categorized by species, type of production, age, and syndrome at the Istituto Zooprofilattico Sperimentale delle Venezie (IZSVe), a multicenter institution for animal health and food safety. We have 72 protocols in use for livestock, poultry, and pets, categorized as, for example, "bovine enteric calf", "rabbit respiratory", "broiler articular". Each protocol consists of a panel of tests, divided into 'mandatory' and 'ancillary', to be selected by the pathologist in order to reach the final diagnosis. After autopsy, the case is categorized into a specific syndrome, subsequently referred to as a syndrome-specific panel of analyses. The activity of the laboratories is monitored through a web-based dynamic reporting system developed using a business intelligence product (QlikView) connected to the laboratory information management system (IZILAB). On a daily basis, reports become available at general, laboratory, and case levels, and are updated as needed. The reporting system highlights epidemiologic variations in the field and allows verification of compliance with the protocols within the organization. The diagnostic protocols are revised annually to increase system efficiency and to address stakeholder requests.
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http://dx.doi.org/10.1177/10406387211003163DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8120072PMC
May 2021

Phenotypic features and phylogenetic background of extraintestinal hemolytic Escherichia coli responsible of mortality in puppies.

Vet Microbiol 2015 Aug 14;179(1-2):126-30. Epub 2015 Mar 14.

Istituto Zooprofilattico Sperimentale delle Venezie, Viale dell'Università 10, 35020 Legnaro, Italy. Electronic address:

A 10-day-old litter of five puppies of Bracco Italiano dog breed showed weakness and diarrhea and, 2 days later, four of them died. At the same time, the bitch showed high hyperthermia (40 °C) and endometritis. The necropsy of a puppy revealed a severe lobar pneumonia accompanied with a bilateral nephrosis. No gross lesions were detected in other organs. Histopathology of the lung revealed severe multifocal fibrino-suppurative necrotizing bronchiolar-alveolitis associated with rod-shaped bacterial aggregates and diffuse interstitial lymphocytic infiltration. The kidney showed severe multifocal necrosis of the tubular epithelium and diffuse severe congestion of the parenchyma. A pure culture of hemolytic Escherichia coli carrying the Cnf-1 gene was identified, from both the puppy organs and bitch's milk. Moreover, phylo-typing assigned them to the phylogroup B2. Two weeks later, fecal samples from the bitch and the survived puppy were collected for a second microbiological analysis, identifying two hemolytic E. coli strains, Cnf positive and Cdt negative and Cnf and Cdt negative, respectively. Some E. coli pathogenic strains may cause enteric or extraintestinal disease. In dogs and cats, strains of extraintestinal pathogenic E. coli (ExPEC) produce specific virulent factors such as hemolysis and cytotoxin necrotizing factors (Cnf). In this episode, we hypothesize that the bitch's milk could be the main source of ExPEC infection causing high puppies mortality. The role of the bitch as a carrier could not be excluded: stressful conditions, such as pregnancy and delivery, would change the host-pathogen dynamics possibly increasing the release of the infectious burden.
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http://dx.doi.org/10.1016/j.vetmic.2015.03.004DOI Listing
August 2015

First reports of Trichinella pseudospiralis in wild boars (Sus scrofa) of Italy.

Vet Parasitol 2011 Jun 18;178(3-4):370-3. Epub 2011 Jan 18.

Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna, Italy.

Trichinella pseudospiralis is a non-encapsulated species infecting both mammals and birds. In Italy, this parasite was reported only in two night-birds of prey of Central Italy. In January 2010, Trichinella larvae were detected in three wild boars (Sus scrofa) of two regions of Northern Italy by enzymatic digestion. The parasites were identified as T. pseudospiralis by multiplex-PCR. The first infected wild boar was hunted in the Emilia Romagna region and the other two infected wild boars were bred outdoors in a small family farm of the Friuli Venezia Giulia region. These new epidemiological data reinforce the role of the wild boar as the main reservoir of T. pseudospiralis in Europe.
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http://dx.doi.org/10.1016/j.vetpar.2011.01.004DOI Listing
June 2011
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