Publications by authors named "Deng Jia"

126 Publications

Development and Validation of a Nomogram Incorporating Colloid Osmotic Pressure for Predicting Mortality in Critically Ill Neurological Patients.

Front Med (Lausanne) 2021 24;8:765818. Epub 2021 Dec 24.

Department of Critical Care Medicine, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China.

The plasma colloid osmotic pressure (COP) values for predicting mortality are not well-estimated. A user-friendly nomogram could predict mortality by incorporating clinical factors and scoring systems to facilitate physicians modify decision-making when caring for patients with serious neurological conditions. Patients were prospectively recruited from March 2017 to September 2018 from a tertiary hospital to establish the development cohort for the internal test of the nomogram, while patients recruited from October 2018 to June 2019 from another tertiary hospital prospectively constituted the validation cohort for the external validation of the nomogram. A multivariate logistic regression analysis was performed in the development cohort using a backward stepwise method to determine the best-fit model for the nomogram. The nomogram was subsequently validated in an independent external validation cohort for discrimination and calibration. A decision-curve analysis was also performed to evaluate the net benefit of the insertion decision using the nomogram. A total of 280 patients were enrolled in the development cohort, of whom 42 (15.0%) died, whereas 237 patients were enrolled in the validation cohort, of which 43 (18.1%) died. COP, neurological pathogenesis and Acute Physiology and Chronic Health Evaluation II (APACHE II) score were predictors in the prediction nomogram. The derived cohort demonstrated good discriminative ability, and the area under the receiver operating characteristic curve (AUC) was 0.895 [95% confidence interval (CI), 0.840-0.951], showing good correction ability. The application of this nomogram to the validation cohort also provided good discrimination, with an AUC of 0.934 (95% CI, 0.892-0.976) and good calibration. The decision-curve analysis of this nomogram showed a better net benefit. A prediction nomogram incorporating COP, neurological pathogenesis and APACHE II score could be convenient in predicting mortality for critically ill neurological patients.
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http://dx.doi.org/10.3389/fmed.2021.765818DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8740271PMC
December 2021

A Novel Small Cyclic Peptide-Based Ga-Radiotracer for Positron Emission Tomography Imaging of PD-L1 Expression in Tumors.

Mol Pharm 2022 Jan 15;19(1):138-147. Epub 2021 Dec 15.

Department of Nuclear Medicine, The Affiliated Hospital of Southwest Medical University, No. 25, Taiping Street, Jiangyang District, Luzhou, Sichuan 646000, People's Republic of China.

In the tumor microenvironment, programmed death protein 1 and programmed death protein ligand 1 (PD-L1) signaling pathways help tumors escape the immune system. We designed a gallium-68 (Ga)-labeled small-molecule peptide-targeting PD-L1 and used positron emission tomography/computed tomography (PET/CT) to detect and dynamically monitor the expression level of PD-L1 in tumors. S-Cyclo(ETSK)-SF-NH (SETSKSF) is a cyclic peptide inhibitor comprising seven amino-acid residues. We connected it with the chelating agent DOTA, labeled DOTA-SETSKSF, with the short half-life nuclide Ga-68, and measured the stability of Ga-2,2',2″-(10-(2-((S)-1-((3S,6S,9S,18S)-18-((S)-1-((S)-1-amino-1-oxo-3-henylpropan-2-ylamino)-3-hydroxy-1-oxopropan-2-ylcarbamoyl)-6-((R)-1-hydroxyethyl)-3-(hydroxymethyl)-2,5,8,12-tetraoxo-1,4,7,13-tetraazacyclooctadecan-9-ylamino)-3-ydroxy-1-oxopropan-2-ylamino)-2-oxoethyl)-1,4,7,10-tetraazacyclododecane-1,4,7-triyl)triacetic acid (Ga-DOTA-SETSKSF) in normal saline (NS), phosphate-buffered saline (PBS), and fetal bovine serum (FBS) . We conducted the Ga-DOTA-SETSKSF affinity test, cell-specific uptake experiments, time-combined experiments, western blotting, and laser confocal experiments to confirm the expression and localization of PD-L1 at the cell level and determine the uptake. Biodistribution and imaging experiments were performed using the H1975, B16F10, and A549 tumor models. Ga-DOTA-SETSKSF was successfully synthesized, and the radiochemical purity was >99% after purification. The stability of Ga-DOTA-SETSKSF was >95% in NS, PBS, and FBS at 37 °C after 4 h of incubation. Cell-binding experiments confirmed that Ga-DOTA-SETSKSF exhibited high uptake in H1975 tumors with high PD-L1 expression and low uptake in A549 tumors with low PD-L1 expression. The clear half-life () of Ga-DOTA-SETSKSF from the blood was 14.48 ± 3.26 min. The percentages of the injected dose per gram of tissue (%ID/g) for H1975 and A549 tumors were 5.29 ± 0.21 and 0.89 ± 0.10 at 1 h after injection, respectively. The H1975 tumor-to-muscle and tumor-to-blood ratios were 41.79 ± 5.81 and 4.75 ± 0.19 at 4 h, respectively. Apart from the H1975 tumor, the kidney and the bladder showed high accumulation because Ga-DOTA-SETSKSF was excreted through the urinary system. PET/CT images showed high accumulation of Ga-DOTA-SETSKSF in H1975 tumors and low uptake in A549 tumors, which was consistent with the results of biodistribution experiments. Ga-DOTA-SETSKSF is convenient to prepare, has high stability, can be used to monitor the expression of PD-L1, and has an extremely high clinical application value.
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http://dx.doi.org/10.1021/acs.molpharmaceut.1c00694DOI Listing
January 2022

Manual Removal versus Spontaneous Delivery of the Placenta at Cesarean Section: A Meta-Analysis of Randomized Controlled Trials.

Ther Clin Risk Manag 2021 2;17:1283-1293. Epub 2021 Dec 2.

Department of Internal Medicine, Eastern Hospital, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, People's Republic of China.

Purpose: Several randomized clinical trials (RCTs) investigated the effects of the manual placental removal on hemorrhage or other hemorrhage-related complications compared with the spontaneous placental removal during cesarean section (CS), while the results remained controversial and were inconsistent. The purpose of this meta-analysis was to quantify the pooled effects of the methods of placental removal on hemorrhage during CS.

Patients And Methods: A systematic literature search was conducted using PubMed, EMBASE, Web of Science, and Google Scholar. Heterogeneity was tested by statistics and Q-statistic. The random-effects model or fixed-effects model were used to calculate the pooled effect for the included studies according to heterogeneity. And the term of standardized mean difference (SMD) with 95% confidence intervals (CI) was pooled and estimated the effects across all studies.

Results: A total of nine RCTs were included in this meta-analysis. Compared with spontaneous group, manual placental removal increased the amount of hemorrhage ( = 0.53, 95% CI [0.12, 0.94]; = 2.54, = 0.011) and increased the risk of endometritis ( = 1.84, 95% CI [1.31, 2.58]; = 3.52, < 0.0001). In contrast, there was no significant difference concerning the operating time ( = -0.30, 95% CI [-0.85, 0.24]; = 1.09, = 0.276), the length of hospital stays ( = 0.11, 95% CI [-0.08, 0.30]; = 1.11, = 0.265), and blood transfusion requirement ( = 1.36, 95% CI [0.91, 2.04]; Z = 1.52, = 0.129), respectively.

Conclusion: Comparing with spontaneous placental removal, manual placental removal appeared to be less positive effect during CS. Because of the limitations of this meta-analysis, more high-quality RCTs are needed to confirm our findings.
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http://dx.doi.org/10.2147/TCRM.S333557DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8648276PMC
December 2021

An online nomogram of acute respiratory distress syndrome originating from pulmonary disease.

Eur J Clin Invest 2021 Nov 9:e13708. Epub 2021 Nov 9.

Department of Respiratory and Critical Care Medicine, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Background: Acute respiratory distress syndrome (ARDS) is a highly heterogeneous disease accompanied by high mortality. Our goal was to investigate the risk factors for 28-day mortality and then establish a predictive online nomogram for ARDS originating from pulmonary disease (ARDSp).

Methods: We examined 1087 patients diagnosed with ARDS from January 2010 to December 2019 at the Second Affiliated Hospital of Chongqing Medical University. A total of 185 ARDSp patients were finally enrolled in the training cohort. A total of 43 ARDSp patients from January 2020 to August 2021 in the Second Affiliated Hospital of Chongqing Medical University and the Traditional Chinese Medical Hospital of Jiangbei District were included in the external validation cohort. Fundamental, clinical and laboratory variables at admission were gathered from medical records, and the 28-day prognosis was followed up.

Results: In the training cohort, it was found that age, sex, C-reactive protein, albumin and multiple organ dysfunction syndrome (MODS) were independent risk factors for 28-day mortality via multivariate logistic regression. The online nomogram software for 28-day mortality showed good discrimination, calibration and clinical utility in both the training cohort and external validation cohort.

Conclusions: For ARDSp patients, older males, lower C-reactive protein and albumin levels, and MODS were independent predictors of a poor 28-day prognosis. The online nomogram based on five independent factors could act as a predictive appliance in clinical practice.
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http://dx.doi.org/10.1111/eci.13708DOI Listing
November 2021

Generation of atomic hydrogen by Ni-Fe hydroxides: Mechanism and activity for hydrodechlorination of trichloroethylene.

Water Res 2021 Dec 26;207:117802. Epub 2021 Oct 26.

School of Civil Engineering, Wuhan University, No. 8, East Lake South Road, Wuhan, PR China; State Key Laboratory of Water Resources and Hydropower Engineering Science, Wuhan University, 430072, China. Electronic address:

Atomic hydrogen (H•) is highly reactive for the hydrodechlorination of trichloroethylene (TCE). In this work, we found that the coprecipitation of Ni and Fe at neutral pH led to an unprecedented catalytic generation of H•. The generated H• effectively dechlorinate TCE to nontoxic ethylene and ethane, and Fe is the only electron donor. The abundant adsorbed H• produced with a Ni/Fe ratio of 0.4 enhances hydrogen evolution reaction causing a low efficiency for hydrodechlorination. In contrast, the active absorbed H• is generated in the crystal lattice of Ni-Fe hydroxides with a Ni/Fe ratio of 3.0 causing highly efficient hydrodechlorination of TCE. This work not only reveals the mechanism of catalytic hydrodechlorination by Ni-Fe hydroxides at neutral pH, but also provides a novel approach to detoxify TCE in contaminated water using facile precipitated Ni-Fe hydroxides.
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http://dx.doi.org/10.1016/j.watres.2021.117802DOI Listing
December 2021

Software for the frontiers of quantum chemistry: An overview of developments in the Q-Chem 5 package.

J Chem Phys 2021 Aug;155(8):084801

Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA.

This article summarizes technical advances contained in the fifth major release of the Q-Chem quantum chemistry program package, covering developments since 2015. A comprehensive library of exchange-correlation functionals, along with a suite of correlated many-body methods, continues to be a hallmark of the Q-Chem software. The many-body methods include novel variants of both coupled-cluster and configuration-interaction approaches along with methods based on the algebraic diagrammatic construction and variational reduced density-matrix methods. Methods highlighted in Q-Chem 5 include a suite of tools for modeling core-level spectroscopy, methods for describing metastable resonances, methods for computing vibronic spectra, the nuclear-electronic orbital method, and several different energy decomposition analysis techniques. High-performance capabilities including multithreaded parallelism and support for calculations on graphics processing units are described. Q-Chem boasts a community of well over 100 active academic developers, and the continuing evolution of the software is supported by an "open teamware" model and an increasingly modular design.
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http://dx.doi.org/10.1063/5.0055522DOI Listing
August 2021

3D Printing and Dissolution Testing of Novel Capsule Shells for Use in Delivering Acetaminophen.

J Pharm Sci 2021 Dec 29;110(12):3829-3837. Epub 2021 Aug 29.

Department of Systems Science and Industrial Engineering, The Thomas J. Watson College of Engineering and Applied Science, Binghamton University, 4400 Vestal Pkwy. E., Binghamton, NY 13902, USA. Electronic address:

Individualized drug delivery improves drug efficacy and safety for patients. To implement individualized drug delivery, patient-specific tailored dosages produced on a small scale are needed. However, current pharmaceutical manufacturing is not suitable for personalized dosage forms. Although convenient to deliver various drugs, current gelatin capsules using animal collagen protein have many limitations, such as releasing drugs too fast and incompatibility with some diets. In contrast, 3D printed capsules have great potential to advance individualized treatments. In this paper, we 3D printed and tested non-animal-based capsule shells for the delivery of acetaminophen. Capsule shells were composed of poly(vinyl) alcohol (PVA) and PVA blends with 5-25% hydroxypropyl methylcellulose (HPMC). Dissolution of acetaminophen when delivered in -hese capsule shells was tested using a USP dissolution test apparatus 2 (paddle type) at gastric pH. The novel shells were compared to each other and to commercially available hard gelatin capsules. Dissolution results show that acetaminophen when delivered in 3D printed capsules was slower than when delivered by gelatin capsules. Increasing the percentage of HPMC in the blend further delayed its release and dissolution. This delay could potentially increase the efficacy and reduce the side effects of acetaminophen. These shells also offer a non-animal-based alternative to gelatin capsules. Furthermore, 3D printing of capsule shells with specific polymer blends may be useful for patient-specific therapy in compounding pharmacies across the country.
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http://dx.doi.org/10.1016/j.xphs.2021.08.030DOI Listing
December 2021

The potential utility of [ Ga]Ga-DOTA-FAPI-04 as a novel broad-spectrum oncological and non-oncological imaging agent-comparison with [F]FDG.

Eur J Nucl Med Mol Imaging 2021 Aug 19. Epub 2021 Aug 19.

Department of Nuclear Medicine, The Affiliated Hospital of Southwest Medical University, No. 25 Taiping St, Jiangyang District, Luzhou, 646000, Sichuan, People's Republic of China.

Purpose: This study aimed to compare the detection performance of [ Ga]Ga-DOTA-FAPI-04 and [F]FDG PET/CT in the patients with various oncological and non-oncological lesions.

Methods: A total of 123 patients underwent contemporaneous [ Ga]Ga-DOTA-FAPI-04 and [F]FDG PET/CT were included in this prospective study. The maximum standard uptake value (SUVmax) was measured to compare oncological and non-oncological lesion uptake. The sensitivity, specificity, predictive values, and accuracy of [F]FDG and [ Ga]Ga-DOTA-FAPI-04 PET/CT for detecting primary, metastatic, and non-oncological lesions were calculated and compared to evaluate the detection efficacy.

Results: The study subjects consisted of 123 patients (69 men and 54 women; mean age 56.11 ± 11.94 years). Among the 102 patients with either newly diagnosed (82 patients) or previously treated solid tumor (20 patients), a total of 88 solid primary malignant tumors in 84/102 patients were detected. Two patients had two primary tumors each and 1 patient had three primary tumors. Among them, 58/102 and 43/102 patients had nodal (376 lesions) and distant metastases (406 lesions), respectively. Eight patients had hematological neoplasm. No malignant oncological diseases were detected in the remaining 13 patients. A total of 145 non-oncological lesions and benign tumors in 52/123 patients were detected incidentally. [ Ga]Ga-DOTA-FAPI-04 PET/CT demonstrated a significantly higher uptake and detection rate for the primary (SUVmax 10.98 ± 5.83 vs. 8.36 ± 6.43, p < 0.001; sensitivity 97.67 vs. 84.89%; and accuracy 96.59 vs. 82.95%, X = 0.538, p = 0.021), nodal (SUVmax 10.50 ± 5.98 vs. 8.20 ± 6.29, p = 0.011; sensitivity 97.59 vs. 84.72%; and accuracy 97.34 vs. 84.31%, X = 2.067, p < 0.001), and distant metastatic lesions (SUVmax 9.64 ± 6.45 vs. 6.74 ± 4.83; p < 0.001; sensitivity 98.01 vs. 65.59%; and accuracy 97.04 vs. 65.51%, X = 4.897, p < 0.001) of solid tumor than did [F]FDG PET/CT. [ Ga]Ga-DOTA-FAPI-04 PET/CT demonstrated a lower activity (SUVmax: 6.84 ± 4.67 vs. 13.09 ± 7.29, p < 0.001) and detection rate (sensitivity 50.65 vs. 96.75%, and accuracy 51.28 vs. 95.51%, X = 5.166, p < 0.001) for multiple myeloma and lymphoma compared to [F]FDG PET/CT. [ Ga]Ga-DOTA-FAPI-04 and [F]FDG PET/CT PET/CT demonstrated a comparative activity (SUVmax 6.40 ± 3.95 vs. 5.74 ± 15.78, p = 0.729) and detection efficacy (sensitivity 86.52 vs. 72.34%, and accuracy 84.83 vs. 72.41%, X = 9.460, p = 0.007) for non-oncological lesion and benign tumor detection.

Conclusions: Except for myeloma and lymphoma, [ Ga]Ga-DOTA-FAPI-04 PET/CT showed a superior detection efficacy for detecting various primary and metastatic lesions than [F]FDG PET/CT. A comparative detection utility for non-oncological lesion was obtained with both tracers. [ Ga]Ga-DOTA-FAPI-04 could be used as a broad-spectrum tumor and inflammatory imaging agent in the clinical especially for various solid tumors and non-oncological lesions.
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http://dx.doi.org/10.1007/s00259-021-05522-wDOI Listing
August 2021

68Ga-FAPI PET/CT Detected Non-FDG-Avid Gastric Stromal Tumor.

Clin Nucl Med 2021 Aug 5. Epub 2021 Aug 5.

From the Department of Nuclear Medicine, The Affiliated Hospital of Southwest Medical University Nuclear Medicine and Molecular Imaging Key Laboratory of Sichuan Province Academician (Expert) Workstation of Sichuan Province Department of Radiology, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, People's Republic of China.

Abstract: We presented a case of 67-year-old man with epigastric discomfort and anorexia for more than 2 months. No abnormal 18F-FDG uptake was found throughout the gastrointestinal tract on 18F-FDG PET/CT. The patient was enrolled in the 68Ga-FAPI PET/CT clinical trial, and a 68Ga-FAPI PET/CT was performed to help detect primary lesion. This examination demonstrated a nodular thickening of the gastric wall with an increased 68Ga-FAPI uptake in the greater curvature of the stomach. Finally, the pathological result confirmed the diagnosis of gastric stromal tumor. This case highlights that 68Ga-FAPI PET/CT might outperform 18F-FDG PET/CT in helping identify gastrointestinal stromal tumors.
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http://dx.doi.org/10.1097/RLU.0000000000003856DOI Listing
August 2021

Electrochemical reductive remediation of trichloroethylene contaminated groundwater using biomimetic iron-nitrogen-doped carbon.

J Hazard Mater 2021 10 24;419:126458. Epub 2021 Jun 24.

Environmental Engineering and Science Program, Department of Chemical and Environmental Engineering, University of Cincinnati, Cincinnati, Ohio 45221-0012, United States.

Electrochemical dechlorination is a prospective strategy to remediate trichloroethylene (TCE)-contaminated groundwater. In this work, iron-nitrogen-doped carbon (FeNC) mimicking microbiological dechlorination coenzymes was developed for TCE removal under environmentally related conditions. The biomimetic FeNC-900, FeNC-1000, and FeNC-1100 materials were synthesized via pyrolysis at different temperatures (900, 1000, and 1100 °C). Due to the synergistic effect of Fe-N active sites and graphitic N sites, FeNC-1000 had the highest electron transfer efficiency and the largest electrochemical active surface area among the as-synthesized FeNC catalysts. The pseudo-first-order rate constants for TCE reduction using FeNC-1000 catalyst are 0.19, 0.28 and 0.36 h at potentials of -0.8 V, -1.0 V and -1.2 V, respectively. Active hydrogen and direct electrons transfer both contribute to the dechlorination from TCE to CH and CH. FeNC maintain a high reactivity after five reuse cycles. Our study provides a novel approach for the dechlorination of chlorinated organic contaminants in groundwater.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126458DOI Listing
October 2021

Facial nerve palsy may indicate coronary artery lesions in Kawasaki disease.

Clin Rheumatol 2021 Oct 31;40(10):4191-4197. Epub 2021 May 31.

Pediatric Neurology Department, Chengdu Women's and Children's Central Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, 611731, China.

Objectives: Facial nerve palsy (FNP) is extremely rare in Kawasaki disease (KD) and the incidence is just 0.9-1.3%. Our objective was to study the clinical features and prognosis of KD accompanied with FNP and to determine whether FNP indicated an increased risk of coronary artery lesions (CALs) in KD.

Methods: An observational study was performed on 9 patients diagnosed as KD accompanied with FNP in Chengdu Women's and Children's Central Hospital, School of Medicine, University of Electronic Science and Technology of China between September 2012 and December 2019. Patients were followed up from September 2012 till September 2020. Clinical features, laboratory and echocardiography findings, treatments, and prognosis were analyzed retrospectively. And the clinical data of patients with FNP (group A, n = 9) were compared to matched KD patients without FNP (group B, n = 27) in order to determine whether FNP indicated an increased risk of CALs in KD. The matching factors included sex, age, days of illness at the intravenous immunoglobulin use, and classic/incomplete KD forms.

Results: Clinical data of 9 patients were analyzed in this study. Patients tended to be younger than 24 months of age (88.9%) and 55.6% were under 12 months. Median onset of FNP was day 10 of illness. Eight patients (88.9%) showed unilateral FNP. The levels of laboratory inflammatory markers (White blood cells count, neutrophil percentage, C-reactive protein, erythrocyte sedimentation rate, etc.) were significantly increased in most cases. CALs were noted in 8 of 9 patients (88.9%). Coronary artery aneurysms occurred in 4 cases (44.4%) and the remaining 4 patients showed coronary artery dilation. All patients were given intravenous immunoglobulin therapy (2 g/kg), aspirin (30-50 mg/kg/day), and short-term dexamethasone. During follow-up, both FNP and CALs were completely recovered in all patients. The duration time of FNP ranged from 10 to 130 days. And the time for CALs to normal ranged from 12 to 282 days. The occurrence of CALs was significantly higher in patients with FNP than those without FNP (88.9% vs. 25.9%, P < 0.05).

Conclusions: KD accompanied with FNP is rare but may indicate an increased risk of CALs. Clinicians should take KD into consideration when children suffer from long-term fever with FNP, even with incomplete diagnostic features. With a long-term follow-up, both FNP and CALs have a good prognosis.

Key Points: • Facial nerve palsy may indicate an increased risk of coronary artery lesions in Kawasaki disease. • Clinicians should take Kawasaki disease into consideration when children suffer from long-term fever with facial nerve palsy, even with incomplete diagnostic features. • Facial nerve palsy and coronary artery lesions have a good prognosis in a long-term follow-up.
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http://dx.doi.org/10.1007/s10067-021-05791-8DOI Listing
October 2021

Role of mass effect and trehalose on early erythrolysis after experimental intracerebral hemorrhage.

J Neurochem 2022 Jan 18;160(1):88-99. Epub 2021 Apr 18.

Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, China.

The mechanisms of brain injury after intracerebral hemorrhage (ICH) involve mass effect-induced primary injury and secondary injury caused by a pathologic response to the hematoma. Considerable attentions have recently been paid to the mechanisms and therapeutic strategy for secondary brain injury due to no overall benefit from early surgery compared with initial conservative treatment. However, it is unclear whether there is a causal relationship between mass effect and secondary brain injury. Here, the role of mass effect on early erythrolysis after experimental ICH was investigated based on the poly(N-isopropylacrylamide) (PNIPAM) ICH model. Autologous blood and PNIPAM hydrogel were co-injected into the right basal ganglia of rats to induce different degrees of mass effect, but with a constant hematoma. The influences of different mass effect and time courses on erythrolysis and brain damages after ICH were investigated. Furthermore, the protective effect of trehalose against erythrolysis after ICH was evaluated. The results showed that mass effect caused erythrocyte morphological change at 24 hr after ICH. The released hemoglobin was quantitatively evaluated by a polynomial concerning with the mass effect, the volume of hematoma, and the time of ICH. An obvious increase in heme oxygenase-1 (HO-1) and ionized calcium binding adaptor molecule-1 (Iba-1) expression, iron deposition, cell death, and neurological deficits was observed with increasing mass effect. Moreover, trehalose alleviated brain injury by inhibiting erythrolysis after ICH. These data demonstrated that mass effect accelerated the erythrolysis and brain damages after ICH, which could be relieved through trehalose therapy.
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http://dx.doi.org/10.1111/jnc.15361DOI Listing
January 2022

Synergistic effect of carboxymethylcellulose and Cryptococcus laurentii on suppressing green mould of postharvest grapefruit and its mechanism.

Int J Biol Macromol 2021 Jun 26;181:253-262. Epub 2021 Mar 26.

Key Laboratory for Forest Resources Conservation and Use in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming 650224, China; Key Laboratory of National Forestry and Grassland Administration on Biodiversity Conservation in Southwest China, Southwest Forestry University, Kunming 650224, China. Electronic address:

The synergistic effects of carboxymethylcellulose (CMC) combined with Cryptococcus laurentii FRUC DJ1 were studied on controlling green mould resulting from Penicillium digitatum in grapefruit fruit. The results indicate that both C. laurentii and the CMC treatment suppressed P. digitatum conidia germination. In addition, C. laurentii growth in vitro was not affected by low CMC concentrations, nevertheless, the biofilm of C. laurentii was enhanced. Compared with the control fruit, the grapefruit had a lower green mould in all treatments. Significantly synergistic effects were caused by combining C. laurentii and CMC on minimum decay incidence and lesion diameter. Combined treatment induced defence enzyme activities, including chitinase, β-1,3-glucanase, peroxidase, polyphenol oxidase, and phenylalanine ammonia-lyase, together with disease tolerance-associated total phenol. Also, this combination inhibited the pathogen growth by adhered to the hyphae and reduced its infection in fruit wounds. Moreover, the commercial quality parameters in the combined treatment of C. laurentii and CMC, including weight loss, total soluble solids, ascorbic acid, and titratable acidity, were superior to single treatment. The combination of C. laurentii and CMC can not only control postharvest decay but also maintain fruit qualities. Thus, it can be used in grapefruit for commercial purposes.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.03.155DOI Listing
June 2021

The microtubule-associated protein WDL4 modulates auxin distribution to promote apical hook opening in Arabidopsis.

Plant Cell 2021 07;33(6):1927-1944

State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China.

The unique apical hook in dicotyledonous plants protects the shoot apical meristem and cotyledons when seedlings emerge through the soil. Its formation involves differential cell growth under the coordinated control of plant hormones, especially ethylene and auxin. Microtubules are essential players in plant cell growth that are regulated by multiple microtubule-associated proteins (MAPs). However, the role and underlying mechanisms of MAP-microtubule modules in differential cell growth are poorly understood. In this study, we found that the previously uncharacterized Arabidopsis MAP WAVE-DAMPENED2-LIKE4 (WDL4) protein plays a positive role in apical hook opening. WDL4 exhibits a temporal expression pattern during hook development in dark-grown seedlings that is directly regulated by ethylene signaling. WDL4 mutants showed a delayed hook opening phenotype while overexpression of WDL4 resulted in enhanced hook opening. In particular, wdl4-1 mutants exhibited stronger auxin accumulation in the concave side of the apical hook. Furthermore, the regulation of the auxin maxima and trafficking of the auxin efflux carriers PIN-FORMED1 (PIN1) and PIN7 in the hook region is critical for WDL4-mediated hook opening. Together, our study demonstrates that WDL4 positively regulates apical hook opening by modulating auxin distribution, thus unraveling a mechanism for MAP-mediated differential plant cell growth.
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http://dx.doi.org/10.1093/plcell/koab080DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8290285PMC
July 2021

Revealing the structural characteristics of lignin macromolecules from perennial ryegrass during different integrated treatments.

Int J Biol Macromol 2021 May 27;178:373-380. Epub 2021 Feb 27.

Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming 650224, China. Electronic address:

To reveal the structural characteristics and physicochemical properties of perennial ryegrass lignin, sequential alkali extractions or double ball-milling and enzymatic hydrolysis on the basis of ultrasonic and hydrothermal pretreatments were proposed in this study. Results revealed that sequential alkali extractions released 89.4% of original lignin from the ryegrass cell walls and 0.75-4.16% of associated carbohydrates as compared to the double ball-milling and enzymatic hydrolysis (96.0% and 18.39%). It was observed that the two types of lignin prepared were SGH-type and had different amounts of p-coumarates and ferulates, and primarily consisted of β-O-4' linkages combined with minor amounts of β-β' and β-5' linkages. Besides, alkali-soluble lignins exhibited relatively fewer β-O-4' linkages, higher S/G ratios and H-type units, and abundant phenolic OH groups as compared to the double enzymatic lignin. Overall, the deeper investigation of the lignin structure of ryegrass will provide useful information for the efficient utilization of lignin macromolecules in biorefineries.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.02.197DOI Listing
May 2021

The Median Effective Dose of Oxytocin Needed to Prevent Uterine Atony During Cesarean Delivery in Elderly Parturients.

Drug Des Devel Ther 2020 8;14:5451-5458. Epub 2020 Dec 8.

Department of Anesthesia, Women's Hospital, Zhejiang University School of Medicine, Hangzhou 310006, People's Republic of China.

Purpose: Oxytocin is the first-line agent to prevent and treat uterine atony during cesarean delivery (CD). We compared the effective dose in 50% of the parturients (ED) of a prophylactic oxytocin bolus during CD in young (<35 years) and old parturients (≥35 years) using Dixon's up-and-down method.

Patients And Methods: Twenty-eight young parturients (young group) and 25 old parturients (old group) undergoing CD under combined spinal-epidural anesthesia were enrolled. The initial oxytocin bolus was 0.5 IU, with increments or decrements of 0.25 IU. Maternal adverse effects, requirement for additional uterotonic agents, and estimated blood loss were recorded.

Results: The ED for oxytocin in the old group was higher than that in the young group (1.41 IU; 95% confidence interval, 0.63-2.19) vs 0.66 IU (0.04-1.29), < 0.001). The total oxytocin dose in the old group was higher than in the young group (5.9 ± 2.9 vs 4.1 ± 2.1 IU, = 0.01). The estimated blood loss in the older group and young group was 401.2 ± 204.5 mL and 289.3 ± 104.6 mL, respectively ( =0.01). The overall prevalence of adverse effects was higher in the old group than in the young group (68.0% vs 21.4%, < 0.001).

Conclusion: The initial bolus and total requirement of oxytocin for preventing uterine atony were higher in old parturients than in young parturients during CD. Advanced maternal age may necessitate higher doses of oxytocin.
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http://dx.doi.org/10.2147/DDDT.S258651DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7737550PMC
September 2021

Age-related changes in endogenous glucocorticoids, gonadal steroids, endocannabinoids and their ratios in plasma and hair from the male C57BL/6 mice.

Gen Comp Endocrinol 2021 01 26;301:113651. Epub 2020 Oct 26.

School of Biological Sciences & Medical Engineering, Southeast University, Nanjing 210096, PR China; Key Laboratory of Child Development and Learning Science (Southeast University), Ministry of Education, Nanjing 210096, PR China; Institute of Child Development and Education, Research Center for Learning Science, Southeast University, Nanjing 210096, PR China. Electronic address:

Age-related level changes of hormones, endocannabinoids and their ratios are of pathophysiological significance for understanding functions, activities and interactions of the endocrine systems, including the hypothalamic-pituitaryadrenal (HPA), hypothalamic-pituitary-gonadal (HPG) axes and endogenous cannabinoid system (ECS). The present study aimed to investigate the age-dependent fluctuations of glucocorticoids, gonadal steroids, endocannabinoids and their ratios from 21 days to 10 months in both plasma and hair from the male C57BL/6 mice. A novel framework based on the liquid chromatography-tandem mass spectrometry was developed to simultaneously determine ten hormones and two endocannabinoids in plasma and hair. Results showed that glucocorticoids, corticosterone (CORT), aldosterone (ALD), 11-dehydrocorticosterone (11-DHC), gonadal steroids, progesterone (P), dehydroepiandrosterone (DHEA), testosterone (T) and dihydrotestosterone (DHT) in plasma were unimodally fluctuated (ps < 0.001) along age with the maximum value at 2.7-month-old. In contrast, the other two gonadal steroids, estrone (E1) and estradiol (E2) were declined with age (ps < 0.001). Differently, endocannabinoids, N-arachidonoyl-ethanolamine (AEA) and 1-arachydonoyl glycerol (1-AG) showed nadir and zenith values at 2.7-month-old and 3.4-month-old, respectively (ps < 0.001). Additionally, the ratios of CORT to 11-DHC and ALD in plasma were dropped similarly with age (ps < 0.001). The ratios of 1-AG to AEA, and of T to A4 and DHT, and of DHEA to A4 were unimodally changed (ps < 0.001) along age with maximum value at 2.7- or 3.4-month-old. In contrast, the ratios of E2 to T and E1 to A4 were decreased with age (ps < 0.05). The rest six ratios that reflected the interactions among the three endocrine systems, were similar age-dependent and showed nadir and zenith values at 2.7-month-old and 3.4-month-old, respectively (ps < 0.05). Most importantly, these findings in light of age-related changing patterns in plasma were repeated in hair, suggesting that the fi41-ndings in the two matrices were mutually validated. However, it was worth noting that their magnitude of levels in the two bio-matrices were markedly different. The current findings could provide reliable hormone and endocannabinoid signatures with age on neuroendocrine profiles as well as their ratios for the male C57BL/6 mice.
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http://dx.doi.org/10.1016/j.ygcen.2020.113651DOI Listing
January 2021

Vascular Complications of Extracorporeal Membrane Oxygenation: A Systematic Review and Meta-Regression Analysis.

Crit Care Med 2020 12;48(12):e1269-e1277

Cardiothoracic Intensive Care Unit, Department of Cardiothoracic and Vascular Surgery, National University Heart Centre, National University Hospital, Singapore.

Objectives: Perform a systematic review and meta-analysis of vascular complications associated with extracorporeal membrane oxygenation and identify prognostic and predictive factors.

Data Sources: Systematic search for publications reporting vascular complications on extracorporeal membrane oxygenation, published from 1972 to January 31, 2020, was conducted via PubMed, Scopus, and Embase.

Study Selection: Of 4,076 references screened, 47 studies with 6,583 patients were included in final analyses. Studies with fewer than 10 patients were excluded.

Data Extraction: Relevant data, including demographics, comorbidities, extracorporeal membrane oxygenation and cannulation characteristics, occurrence rates of early and late vascular complications, patient outcomes, and use of distal perfusion cannula, were extracted from selected articles into an excel sheet specifically designed for this review.

Data Synthesis: Random-effects meta-analyses and meta-regression analyses were undertaken. Overall pooled estimate of vascular complications in our meta-analysis was 29.5% (95% CI, 23.6-35.9%). Two-thousand three-hundred forty-seven vascular complications in 6,124 venoarterial extracorporeal membrane oxygenation patients compared with 95 in 459 venovenous extracorporeal membrane oxygenation patients (odds ratio, 2.35; 95% CI, 1.87-2.96; p < 0.0001) were analyzed. Successful weaning off extracorporeal membrane oxygenation occurred in 60.6% of pooled patients; 46.2% were eventually discharged. Pooled prevalences of vascular complications like significant bleeding, limb ischemia, and cannula site bleeding were 15.4% (95% CI, 8.6-23.7%), 12.6% (95% CI, 10.0-15.5%), and 12.6% (95% CI, 9.6-18.5%), respectively. Meta-analysis showed that the use of distal perfusion cannula was associated with lower odds of limb ischemia (odds ratio, 1.93; 95% CI, 1.17-2.47; p = 0.03) Meta-regression showed that male sex, smoking, advanced age, and comorbidities contributed to higher in-hospital mortality, while distal perfusion cannula was protective.

Conclusions: Nearly a third of patients on extracorporeal membrane oxygenation develop vascular complications; elderly males with comorbidities appear vulnerable. The use of distal perfusion cannulas caused significant reduction in limb ischemia and mortality.
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http://dx.doi.org/10.1097/CCM.0000000000004688DOI Listing
December 2020

Modeling organically fertilized flooded rice systems and its long-term effects on grain yield and methane emissions.

Sci Total Environ 2021 Feb 1;755(Pt 2):142578. Epub 2020 Oct 1.

Department of Soil and Crop Sciences, Texas A&M University, College Station, TX 77843, USA.

The increasing trend of adopting organic fertilization in rice production can impact grain yields and soil methane (CH) emissions. To simulate these impacts in the absence of long-term field data, a process-based biogeochemical model, Denitrification and Decomposition (DNDC version 9.5) was used. The model was calibrated against a single year greenhouse study and validated using a previously published one-year field trial from 1990, both comparing varying fertilization systems in rice production in southeast Texas, USA. In both the greenhouse and the field studies, lower grain yield and greater soil CH emissions were observed in organically fertilized systems. Calibrated model simulations of the greenhouse study correlated with the observed daily CH emissions (conventional r = 0.87; organic r = 0.91) and SOC (r = 0.83); but, the model overestimated yield of conventional systems (slope = 1.2) and underestimated yield of organic systems (slope = 0.68). For the field study, agreement between simulated and observed yields and CH emissions resulted in slopes close to 1. A simple organic system with urea and straw amendment from the field study was an input available in DNDC whereas the slow release, pelletized organic fertilizer used in the greenhouse study, Nature Safe, was not modeled well by DNDC. The validated model was used to simulate 22 years of rice production and predicted that the differences in yield and CH emissions between treatments would diminish with time. In the model simulations, the overall soil health was enhanced when managed with organic fertilization compared to conventional inorganic fertilizers. Model simulations could be improved further by including site-specific calibration of soil organic C, and soil carbon dioxide (CO) emissions.
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http://dx.doi.org/10.1016/j.scitotenv.2020.142578DOI Listing
February 2021

Global Research Alliance N O chamber methodology guidelines: Summary of modeling approaches.

J Environ Qual 2020 Sep 27;49(5):1168-1185. Epub 2020 Aug 27.

AgResearch Lincoln Research Centre, PB 4749, Christchurch, 8140, New Zealand.

Measurements of nitrous oxide (N O) emissions from agriculture are essential for understanding the complex soil-crop-climate processes, but there are practical and economic limits to the spatial and temporal extent over which measurements can be made. Therefore, N O models have an important role to play. As models are comparatively cheap to run, they can be used to extrapolate field measurements to regional or national scales, to simulate emissions over long time periods, or to run scenarios to compare mitigation practices. Process-based models can also be used as an aid to understanding the underlying processes, as they can simulate feedbacks and interactions that can be difficult to distinguish in the field. However, when applying models, it is important to understand the conceptual process differences in models, how conceptual understanding changed over time in various models, and the model requirements and limitations to ensure that the model is well suited to the purpose of the investigation and the type of system being simulated. The aim of this paper is to give the reader a high-level overview of some of the important issues that should be considered when modeling. This includes conceptual understanding of widely used models, common modeling techniques such as calibration and validation, assessing model fit, sensitivity analysis, and uncertainty assessment. We also review examples of N O modeling for different purposes and describe three commonly used process-based N O models (APSIM, DayCent, and DNDC).
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http://dx.doi.org/10.1002/jeq2.20119DOI Listing
September 2020

Effect of traditional Chinese medicine injections on severe pneumonia: A protocol for systematic review and meta-analysis.

Medicine (Baltimore) 2020 Sep;99(39):e22012

Department of Respiratory and Critical Care Medicine, Chongqing Jiangbei Hospital of Traditional Chinese Medicine.

Background: Traditional Chinese medicine injections (TCMJ) used in the treatment of severe pneumonia have been widely implemented in clinical practice, but their overall efficacy and safety remain unclear. This paper aims to evaluate the efficacy and safety of TCMJ in the treatment of severe pneumonia.

Methods: PubMed, EMbase, Cochrane Library, Web of Science, China National Knowledge Infrastructure, WanFang, and the Chongqing VIP Chinese Science and Technology Periodical Database were all searched for randomized controlled trials focusing on the administration of TCMJ for severe pneumonia. Two researchers independently screened titles, abstracts, full texts, and extracted relevant data. The RevMan 5.3 software (The Cochrane Collaboration, Software Update, Oxford, UK) and Stata 14 software (STATA Corporation, College Station, TX) were used for statistical analysis.

Results: This study summarizes the related randomized controlled trials to assess the effect and safety of TCMJ in the treatment of severe pneumonia.

Conclusion: This article provides theoretical support for the clinical application of TCMJ in the treatment of severe pneumonia.

Prospero Registration Number: CRD42020185072.
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http://dx.doi.org/10.1097/MD.0000000000022012DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7523816PMC
September 2020

Time to positive culture can differentiate post-neurosurgical coagulase-negative Staphylococci other than S epidermidis meningitis from contamination: A case-control observational study.

J Clin Lab Anal 2020 Oct 7;34(10):e23447. Epub 2020 Jul 7.

Department of Clinical Diagnosis, Laboratory of Beijing Tiantan Hospital and Capital Medical University, Beijing, China.

Objective: To explore the characteristics of coagulase-negative Staphylococci other than Staphylococci epidermidis (Nse-CoNS) meningitis and to apply cerebrospinal fluid (CSF) times to positivity culture (TTPC) for the precise differentiation of meningitis from contamination.

Methods: We conducted a case-control study to accomplish the following: First, we retrospectively reviewed records of post-neurosurgical patients' CSF that yielded Nse-CoNS from January to October 2019 at the Beijing Tiantan Hospital; 17 clinical and 12 laboratory characteristics were reviewed. Second, we investigated the TTPC of the Nse-CoNS, the cutoffs, and corresponding parameters to differentiate Nse-CoNS meningitis from contamination.

Results: In this study, a total of 146 patients with Nse-CoNS CSF culture positive were enrolled. The average TTPC in the Nse-CoNS meningitis group was significantly shorter than in the contamination group (20.2 ± 5.0 hours and 30.2 ± 12.6 hours, respectively, P < .05). The area under curve (AUC) of the model was 0.802. A TTPC of 20.0 hours had 94.3% sensitivity and a negative value of 90.2% for predicting Nse-CoNS meningitis.

Conclusions: Nse-CoNS meningitis often causes confusion in clinical diagnosis. In this study, we evaluated the clinical predictive factors of Nse-CoNS meningitis and confirmed that the median TTPC in the Nse-CoNS meningitis group was significantly shorter than in the contamination group. A TTPC shorter than 20.0 hours was associated with Nse-CoNS meningitis, while a TTPC longer than 20.0 hours was associated with Nse-CoNS contamination. This information will be helpful for the rapid diagnosis of Nse-CoNS meningitis.
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http://dx.doi.org/10.1002/jcla.23447DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7595912PMC
October 2020

Progressive nitrogen limitation across the Tibetan alpine permafrost region.

Nat Commun 2020 07 3;11(1):3331. Epub 2020 Jul 3.

State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.

The ecosystem carbon (C) balance in permafrost regions, which has a global significance in understanding the terrestrial C-climate feedback, is significantly regulated by nitrogen (N) dynamics. However, our knowledge on temporal changes in vegetation N limitation (i.e., the supply of N relative to plant N demand) in permafrost ecosystems is still limited. Based on the combination of isotopic observations derived from a re-sampling campaign along a ~3000 km transect and simulations obtained from a process-based biogeochemical model, here we detect changes in ecosystem N cycle across the Tibetan alpine permafrost region over the past decade. We find that vegetation N limitation becomes stronger despite the increased available N production. The enhanced N limitation on vegetation growth is driven by the joint effects of elevated plant N demand and gaseous N loss. These findings suggest that N would constrain the future trajectory of ecosystem C cycle in this alpine permafrost region.
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http://dx.doi.org/10.1038/s41467-020-17169-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7335038PMC
July 2020

A Novel Sox9/lncRNA H19 Axis Contributes to Hepatocyte Death and Liver Fibrosis.

Toxicol Sci 2020 09;177(1):214-225

State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, Nanjing University.

Sox9 has been previously characterized as a transcription factor responsible for the extracellular matrix production during liver fibrosis. However, the deregulation and functional role of hepatocyte Sox9 in the progression of liver fibrosis remains elusive. Here, we found a significant increase of Sox9 in the hepatocytes isolated from CCl4-induced fibrotic liver and showed that antisense oligoribonucleotides depletion of Sox9 was sufficient to attenuate CCl4-induced liver fibrosis. Notably, the increase of Sox9 in hepatocyte was associated with the upregulation of long noncoding RNA H19 in both in vitro and in vivo systems. Mechanistic studies revealed that Sox9 induced H19 by binding to a conserved promoter region of H19. In vitro, hepatocyte injury triggered the increase of Sox9/H19 axis, whereas silence of H19 greatly alleviated the H2O2-induced hepatocyte apoptosis, suggesting that H19 functions as a downstream effector of Sox9 signaling and is involved in hepatocyte apoptosis. In animal experiments, inhibition of H19 alleviated the activation of hepatic stellate cells and reduced the extent of liver fibrosis, whereas ectopic expression of H19 abolished the inhibitory effects of Sox9 depletion on liver fibrosis, suggesting that the profibrotic effect of hepatocyte Sox9 depends on H19. Finally, we investigated the clinical relevance of Sox9/H19 axis to liver fibrosis and identified the increase of Sox9/H19 axis in liver cirrhosis patients. In conclusion, our findings link Sox9/H19 axis to the intrinsic mechanisms of hepatocyte apoptosis and may represent a hitherto unknown paradigm in hepatocyte injury associated with the progression of liver fibrosis.
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http://dx.doi.org/10.1093/toxsci/kfaa097DOI Listing
September 2020

An increase in alveolar fluid clearance induced by hyperinsulinemia in obese rats with LPS-induced acute lung injury.

Respir Physiol Neurobiol 2020 08 28;279:103470. Epub 2020 May 28.

Department of Respiratory Medicine, Traditional Chinese Medical Hospital of Jiangbei District, Chongqing, China.

A lower mortality rate is observed in obese patients with acute lung injury (ALI), which is referred to as the obesity paradox, in several studies and recent meta-analyses. Hyperinsulinemia is characterized as the primary effect of obesity, and exogenous insulin attenuates LPS-induced pulmonary edema. The detailed mechanism responsible for the effect of hyperinsulinemia on pulmonary edema and alveolar filling needs to be elucidated. SD rats were fed with a high-fat diet (HFD) for a total of 14 weeks. SD rats were anesthetized and intraperitoneally injected with 10 mg/kg lipopolysaccharide (LPS), while control rats received only saline vehicle. Insulin receptor antagonist S961 (20 nmol/kg) was given by the tail vein and serum, and glucocorticoid-induced protein kinase-1 (SGK-1) inhibitor EMD638683 (20 mg/kg) was administrated intragastrically prior to LPS exposure. The lungs were isolated for the measurement of alveolar fluid clearance. The protein expression of epithelial sodium channel (ENaC) was detected by Western blot. Insulin level in serum was significantly higher in HFD rats compared with normal diet rats in the presence or absence of LPS pretreatment. Hyperinsulinemia induced by high fat feeding increased alveolar fluid clearance and the abundance of α-ENaC, β-ENaC, and γ-ENaC in both normal rats and ALI rats. Moreover, these effects were reversed in response to S961. EMD638683 prevented the simulation of alveolar fluid clearance and protein expression of ENaC in HFD rats with ALI. These findings suggest that hyperinsulinemia induced by obesity results in the stimulation of alveolar fluid clearance via the upregulation of the abundance of ENaC in clinical acute lung injury, whereas theses effects are prevented by an SGK-1 inhibitor.
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http://dx.doi.org/10.1016/j.resp.2020.103470DOI Listing
August 2020

Lidocaine mediates the progression of cerebral ischemia/reperfusion injury in rats via inhibiting the activation of NF-κB p65 and p38 MAPK.

Ann Transl Med 2020 Apr;8(8):548

Department of Anesthesiology, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, Chengdu 610072, China.

Background: Lidocaine is a commonly used local anesthetic, and low-dose lidocaine has neuroprotective effects on cerebral ischemia/reperfusion (CI/R) injury; the mechanism for this, however, is still unclear. The aim of this study was to investigate the role and the possible mechanisms of lidocaine on CI/R injury in rats.

Methods: We constructed a rat (male Sprague-Dawley rats, 6-8 weeks old) model of CI/R injury induced by middle cerebral artery occlusion (MCAO). Histopathology, neuronal apoptosis, oxidative stress, and inflammatory response were evaluated using hematoxylin and eosin (HE) staining, Nissl staining, enzyme-linked immunosorbent assay (ELISA) and western blotting, respectively. In addition, brain water content, infarct volume, neurological deficit score each evaluated.

Results: The findings showed that lidocaine improved spatial learning and memory impairment, protected I/R-induced brain injury and attenuated neuronal death and apoptosis. Furthermore, lidocaine also regulated the levels of malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD), IL-6, IL-10, iNOS, and IL-4.Notably, lidocaine markedly inhibited the expression of p65 and p38.

Conclusions: The results indicate that lidocaine protects against cerebral injury induced by I/R in rats via the nuclear factor kappa-B (NF-κB) p65 and p38 mitogen-activated protein kinase (MAPK) signaling pathway, it provided a candidate for the treatment of CI/R-induced injury.
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http://dx.doi.org/10.21037/atm-20-3066DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7214891PMC
April 2020

Insight into the Regulatory Function of Human Hair Keratins in Wound Healing Using Proteomics.

Adv Biosyst 2020 06 16;4(6):e1900235. Epub 2020 Apr 16.

Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, 400030, China.

Keratins derived from human hair possess excellent wound healing qualities. However, their functional contribution to this process is poorly understood. In this study, the regulatory function of human hair keratins in wound healing is investigated using proteomic analysis by dividing keratins into different groups based on their molecular weight distributions: low molecular weight keratins (LMWK, 10-30 kDa), medium molecular weight keratins (MMWK, 30-50 kDa), and high molecular weight keratins (HMWK, >50 kDa). Keratin hydrogels with different molecular weights exhibit various morphologies, rheological properties, degradation rates, and wound healing activities. Using proteomic analysis, LMWK and HMWK hydrogels exhibit a stronger regulatory ability for wound healing at days 1 and 7, respectively. The major functions of LMWK during wound healing are regulation of cells communication and function. In contrast, proteins associated with energy metabolism are significantly expressed after HMWK hydrogel treatment at day 1, and these play an important role in cellular growth and reactive oxygen species scavenging at day 7. These results demonstrate that the wound healing qualities of human hair keratins are influenced by their molecular weight distribution, and the proteomic analysis sheds new light on the regulatory function of human hair keratins during wound healing.
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http://dx.doi.org/10.1002/adbi.201900235DOI Listing
June 2020

Autopsy examination after extracorporeal membrane oxygenation: 'Mortuis vivos docent'.

J Thorac Dis 2020 Mar;12(3):1121-1123

Cardiothoracic Intensive Care Unit, National University Heart Centre, National University Hospital, Singapore.

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http://dx.doi.org/10.21037/jtd.2020.01.55DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7139053PMC
March 2020

A comparison of reproductive outcomes of patients with adenomyosis and infertility treated with High-Intensity focused ultrasound and laparoscopic excision.

Int J Hyperthermia 2020 ;37(1):301-307

Department of Gynecology, Third Xiangya Hospital of Central South University, Changsha, China.

The aim of this study was to compare the treatment effects of high-intensity focused ultrasound (HIFU) and laparoscopic excision (LE) in patients with adenomyosis and infertility. A total of 93 patients with adenomyosis and infertility who were treated with HIFU (50 patients) or LE (43 patients) from January 2012 to January 2017 at the Third Xiangya Hospital of Central South University were retrospectively analyzed. Clinical characteristics including dysmenorrhea severity pain score, menorrhagia severity scores, reproductive outcomes, complications during pregnancy and delivery, adverse effects, surgical complications, and other clinical variables were compared between the HIFU and LE groups. Of the total 93 patients with adenomyosis and infertility, 50 were treated with HIFU and 43 underwent LE. Both HIFU and LE treatments achieved significant relief of dysmenorrhea and menorrhagia. The total hospital stay was shorter in patients treated with HIFU than in those who underwent LE surgery. Neither HIFU nor LE treatment led to severe complications after treatment. Most importantly, patients treated with HIFU showed significantly higher pregnancy rates and natural conception rates than those who underwent LE surgery. Notably, in the HIFU treatment group, those with diffuse adenomyotic lesions had significantly lower postoperative pregnancy rates than those with focal adenomyosis. HIFU showed a safe and effective profile as a therapeutic management option for patients with adenomyosis. In comparison with LE, HIFU treatment achieved better postoperative reproductive outcomes. HIFU treatment should be encouraged and implemented in clinical practice.
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http://dx.doi.org/10.1080/02656736.2020.1742390DOI Listing
October 2020

The influence of an electrical impedance tomography belt on lung function determined by spirometry in sitting position.

Physiol Meas 2020 05 4;41(4):044002. Epub 2020 May 4.

Department of Respiratory Rehabilitation Center, Beijing Rehabilitation Hospital of Capital Medical University, Xixiazhuang, Badachu, Shijingshan, Beijing 100114, People's Republic of China. Na Zhang and Hongying Jiang contributed equally to this work.

Objective: The aim of the study was to examine whether an electrical impedance tomography (EIT) electrode belt changed the lung function in healthy volunteers and patients with respiratory muscle weakness (RMW) and chronic obstructive pulmonary disease (COPD).

Approach: In total, thirty subjects were included (10 healthy volunteers, 10 subjects with RMW, maximum inspiratory pressure < 40 cmHO, and 10 COPD, grade I-IV). Spirometry measurements were conducted in a sitting position once a day at similar times on two consecutive days. Slow expiratory vital capacity (VC), forced vital capacity (FVC) and maximum voluntary ventilation (MVV) manoeuvres were performed. On day 1, spirometry was performed without the EIT electrode belt, and on day 2, the belt was attached to the thorax.

Main Results: Lung function was not influenced by the electrode belt in healthy subjects. The test-retest reliability in the healthy group was 0.89, 0.89 and 0.85 for VC, FVC and MVV, respectively. On the other hand, all investigated parameters were significantly decreased in the RMW group (VC, 51.3 ± 18.0 versus 46.5 ± 18.0% predicted, without versus with EIT belt, p< 0.01; FVC, 51.7 ± 19.0 versus 45.8 ± 18.1% predicted, p< 0.01; MVV, 41.0 ± 20.0 versus 38.8 ± 19.6% predicted, p< 0.01). VC and MVV also decreased significantly in the COPD group (VC, 77.4 ± 20.5 versus 74.6 ± 18.8% predicted, p< 0.05; MVV, 57.4 ± 15.7 versus 54.4 ± 12.5% predicted, p< 0.05).

Significance: An EIT electrode belt could reduce lung volumes in subjects with pre-existing lung diseases. Comparing lung function acquired with an electrode belt to corresponding values obtained without the belt should be avoided.
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http://dx.doi.org/10.1088/1361-6579/ab7edbDOI Listing
May 2020
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