Deepak Reyon

Deepak Reyon

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Deepak Reyon

Deepak Reyon

Publications by authors named "Deepak Reyon"

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40Publications

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Disruption of results in myeloproliferative neoplasms in zebrafish.

Dis Model Mech 2019 05 7;12(5). Epub 2019 May 7.

Department of Pediatric Oncology, Dana-Farber Cancer Institute, Harvard Medical School, Massachusetts 02215, USA

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http://dx.doi.org/10.1242/dmm.035790DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6550042PMC
May 2019

Targeted Mutagenesis in Zebrafish Using CRISPR RNA-Guided Nucleases.

Methods Mol Biol 2015 ;1311:317-34

Cardiovascular Research Center, Massachusetts General Hospital, Charlestown, MA, 02129, USA.

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http://dx.doi.org/10.1007/978-1-4939-2687-9_21DOI Listing
February 2016

Targeted genome editing in human cells using CRISPR/Cas nucleases and truncated guide RNAs.

Methods Enzymol 2014 ;546:21-45

Molecular Pathology Unit, Center for Computational and Integrative Biology, and Center for Cancer Research, Massachusetts General Hospital, Charlestown, Massachusetts, USA; Department of Pathology, Harvard Medical School, Boston, Massachusetts, USA. Electronic address:

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http://dx.doi.org/10.1016/B978-0-12-801185-0.00002-7DOI Listing
July 2015

Context influences on TALE-DNA binding revealed by quantitative profiling.

Nat Commun 2015 Jun 11;6:7440. Epub 2015 Jun 11.

1] Division of Genetics, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA [2] Committee on Higher Degrees in Biophysics, Harvard University, Cambridge, Massachusetts 02138, USA [3] Harvard-MIT Division of Health Sciences and Technology (HST), Harvard Medical School, Boston, Massachusetts 02115, USA [4] Department of Pathology, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA.

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http://dx.doi.org/10.1038/ncomms8440DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4467457PMC
June 2015

Targeted disruption of DNMT1, DNMT3A and DNMT3B in human embryonic stem cells.

Nat Genet 2015 May 30;47(5):469-78. Epub 2015 Mar 30.

1] Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA. [2] Harvard Stem Cell Institute, Cambridge, Massachusetts, USA. [3] Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, Massachusetts, USA.

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http://dx.doi.org/10.1038/ng.3258DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4414868PMC
May 2015

IκB kinase β (IKBKB) mutations in lymphomas that constitutively activate canonical nuclear factor κB (NFκB) signaling.

J Biol Chem 2014 Sep 8;289(39):26960-72. Epub 2014 Aug 8.

From the Center for Cancer Research, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02129,

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http://www.jbc.org/lookup/doi/10.1074/jbc.M114.598763
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http://dx.doi.org/10.1074/jbc.M114.598763DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4175336PMC
September 2014

Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing.

Nat Biotechnol 2014 Jun 25;32(6):569-76. Epub 2014 Apr 25.

1] Molecular Pathology Unit, Massachusetts General Hospital, Charlestown, Massachusetts, USA. [2] Center for Cancer Research, Massachusetts General Hospital, Charlestown, Massachusetts, USA. [3] Center for Computational and Integrative Biology, Massachusetts General Hospital, Charlestown, Massachusetts, USA. [4] Department of Pathology, Harvard Medical School, Boston, Massachusetts, USA.

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http://dx.doi.org/10.1038/nbt.2908DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4090141PMC
June 2014

Broad specificity profiling of TALENs results in engineered nucleases with improved DNA-cleavage specificity.

Nat Methods 2014 Apr 16;11(4):429-35. Epub 2014 Feb 16.

1] Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts, USA. [2] Howard Hughes Medical Institute, Harvard University, Cambridge, Massachusetts, USA.

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http://dx.doi.org/10.1038/nmeth.2845DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4010127PMC
April 2014

Improving CRISPR-Cas nuclease specificity using truncated guide RNAs.

Nat Biotechnol 2014 Mar 26;32(3):279-284. Epub 2014 Jan 26.

Molecular Pathology Unit, Massachusetts General Hospital, Charlestown, MA, USA.

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http://dx.doi.org/10.1038/nbt.2808DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3988262PMC
March 2014

Heritable and precise zebrafish genome editing using a CRISPR-Cas system.

PLoS One 2013 9;8(7):e68708. Epub 2013 Jul 9.

Cardiovascular Research Center, Massachusetts General Hospital, Charlestown, Massachusetts, United States of America.

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0068708PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3706373PMC
February 2014

Locus-specific editing of histone modifications at endogenous enhancers.

Nat Biotechnol 2013 Dec 8;31(12):1133-6. Epub 2013 Sep 8.

1] Howard Hughes Medical Institute, Chevy Chase, Maryland, USA. [2] Department of Pathology and Center for Cancer Research, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA. [3] Broad Institute of Harvard and MIT, Cambridge Massachusetts, USA. [4] Center for Systems Biology, Massachusetts General Hospital, Boston, Massachusetts, USA. [5].

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http://dx.doi.org/10.1038/nbt.2701DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3858395PMC
December 2013

Targeted DNA demethylation and activation of endogenous genes using programmable TALE-TET1 fusion proteins.

Nat Biotechnol 2013 Dec 9;31(12):1137-42. Epub 2013 Oct 9.

1] Department of Pathology and Center for Cancer Research, Massachusetts General Hospital, Charlestown, Massachusetts, USA. [2] Center for Computational and Integrative Biology, Massachusetts General Hospital, Charlestown, Massachusetts, USA. [3] Program in Biological and Biomedical Sciences, Harvard Medical School, Boston, Massachusetts, USA. [4].

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http://www.nature.com/articles/nbt.2726
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http://dx.doi.org/10.1038/nbt.2726DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3858462PMC
December 2013

In silico abstraction of zinc finger nuclease cleavage profiles reveals an expanded landscape of off-target sites.

Nucleic Acids Res 2013 Oct 14;41(19):e181. Epub 2013 Aug 14.

Molecular Pathology Unit, Center for Cancer Research, Massachusetts General Hospital, Charlestown, MA 02129, USA, Center for Computational and Integrative Biology, Massachusetts General Hospital, Charlestown, MA 02129, USA, Department of Pathology, Harvard Medical School, Boston, MA 02115, USA, Program in Biological and Biomedical Sciences, Harvard Medical School, Boston, MA 02115, USA, Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 01238, USA, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA and Howard Hughes Medical Institute, Chevy Chase, MD 02815, USA.

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http://dx.doi.org/10.1093/nar/gkt716DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3799455PMC
October 2013

Targeted deletion and inversion of tandemly arrayed genes in Arabidopsis thaliana using zinc finger nucleases.

G3 (Bethesda) 2013 Oct 3;3(10):1707-15. Epub 2013 Oct 3.

Department of Genetics, Cell Biology & Development and Center for Genome Engineering, University of Minnesota, Minneapolis, Minnesota 55455.

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http://dx.doi.org/10.1534/g3.113.006270DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3789795PMC
October 2013

High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells.

Nat Biotechnol 2013 Sep 23;31(9):822-6. Epub 2013 Jun 23.

1] Molecular Pathology Unit, Massachusetts General Hospital, Charlestown, Massachusetts, USA. [2] Center for Cancer Research, Massachusetts General Hospital, Charlestown, Massachusetts, USA. [3] Center for Computational and Integrative Biology, Massachusetts General Hospital, Charlestown, Massachusetts, USA. [4] Department of Pathology, Harvard Medical School, Boston, Massachusetts, USA.

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http://dx.doi.org/10.1038/nbt.2623DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3773023PMC
September 2013

Engineering customized TALE nucleases (TALENs) and TALE transcription factors by fast ligation-based automatable solid-phase high-throughput (FLASH) assembly.

Curr Protoc Mol Biol 2013 Jul;Chapter 12:Unit 12.16

Molecular Pathology Unit, Center for Computational and Integrative Biology, Massachusetts General Hospital, Charlestown, Massachusetts, USA.

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http://dx.doi.org/10.1002/0471142727.mb1216s103DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3767754PMC
July 2013

Efficient genome editing in zebrafish using a CRISPR-Cas system.

Nat Biotechnol 2013 Mar 29;31(3):227-9. Epub 2013 Jan 29.

Cardiovascular Research Center, Massachusetts General Hospital, Charlestown, Massachusetts, USA.

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http://dx.doi.org/10.1038/nbt.2501DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3686313PMC
March 2013

Robust, synergistic regulation of human gene expression using TALE activators.

Nat Methods 2013 Mar 10;10(3):243-5. Epub 2013 Feb 10.

Molecular Pathology Unit, Massachusetts General Hospital, Charlestown, Massachusetts, USA.

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http://dx.doi.org/10.1038/nmeth.2366DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3584229PMC
March 2013

Engineering designer transcription activator-like effector nucleases (TALENs) by REAL or REAL-Fast assembly.

Curr Protoc Mol Biol 2012 Oct;Chapter 12:Unit 12.15

Molecular Pathology Unit, Center for Computational and Integrative Biology, Massachusetts General Hospital, Charlestown, Massachusetts, USA.

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http://dx.doi.org/10.1002/0471142727.mb1215s100DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3753116PMC
October 2012

Improved somatic mutagenesis in zebrafish using transcription activator-like effector nucleases (TALENs).

PLoS One 2012 24;7(5):e37877. Epub 2012 May 24.

Molecular Pathology Unit and Center for Cancer Research, Massachusetts General Hospital, Charlestown, Massachusetts, United States of America.

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0037877PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3360007PMC
September 2012

Highly efficient generation of heritable zebrafish gene mutations using homo- and heterodimeric TALENs.

Nucleic Acids Res 2012 Sep 7;40(16):8001-10. Epub 2012 Jun 7.

Cardiovascular Research Center, Massachusetts General Hospital, Department of Medicine, Harvard Medical School, Charlestown, MA 02129, USA.

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http://dx.doi.org/10.1093/nar/gks518DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3439908PMC
September 2012

FLASH assembly of TALENs for high-throughput genome editing.

Nat Biotechnol 2012 May;30(5):460-5

Molecular Pathology Unit, Center for Computational and Integrative Biology, and Center for Cancer Research, Massachusetts General Hospital, Charlestown, Massachusetts, USA.

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http://www.nature.com/articles/nbt.2170
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http://dx.doi.org/10.1038/nbt.2170DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3558947PMC
May 2012

Predicting success of oligomerized pool engineering (OPEN) for zinc finger target site sequences.

BMC Bioinformatics 2010 Nov 2;11:543. Epub 2010 Nov 2.

Molecular Pathology Unit, Center for Cancer Research, and Center for Computational and Integrative Biology, Massachusetts General Hospital, Charlestown, MA 02129, USA.

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http://dx.doi.org/10.1186/1471-2105-11-543DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3098093PMC
November 2010

ZiFiT (Zinc Finger Targeter): an updated zinc finger engineering tool.

Nucleic Acids Res 2010 Jul 30;38(Web Server issue):W462-8. Epub 2010 Apr 30.

Molecular Pathology Unit, Center for Cancer Research, and Center for Computational and Integrative Biology, Massachusetts General Hospital, Charlestown, MA 02129, USA.

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http://dx.doi.org/10.1093/nar/gkq319DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2896148PMC
July 2010

High frequency targeted mutagenesis in Arabidopsis thaliana using zinc finger nucleases.

Proc Natl Acad Sci U S A 2010 Jun 27;107(26):12028-33. Epub 2010 May 27.

Department of Genetics, Cell Biology, and Development and Center for Genome Engineering, University of Minnesota, Minneapolis, MN 55455, USA.

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http://dx.doi.org/10.1073/pnas.0914991107DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2900673PMC
June 2010

Rapid mutation of endogenous zebrafish genes using zinc finger nucleases made by Oligomerized Pool ENgineering (OPEN).

PLoS One 2009 9;4(2):e4348. Epub 2009 Feb 9.

Molecular Pathology Unit, Center for Cancer Research, and Center for Computational and Integrative Biology, Massachusetts General Hospital, Charlestown, Massachusetts, United States of America.

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0004348PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2634973PMC
April 2009