Publications by authors named "De-yu Zhang"

10 Publications

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NFATc3-dependent expression of miR-153-3p promotes mitochondrial fragmentation in cardiac hypertrophy by impairing mitofusin-1 expression.

Theranostics 2020 1;10(2):553-566. Epub 2020 Jan 1.

Center for Developmental Cardiology, Institute for Translational Medicine, College of Medicine, Qingdao University, Qingdao 266021, China.

Mitochondrial dysfunction is involved in the pathogenesis of various cardiovascular disorders. Although mitochondrial dynamics, including changes in mitochondrial fission and fusion, have been implicated in the development of cardiac hypertrophy, the underlying molecular mechanisms remain mostly unknown. Here, we show that NFATc3, miR-153-3p, and mitofusion-1 (Mfn1) constitute a signaling axis that mediates mitochondrial fragmentation and cardiomyocyte hypertrophy. Isoprenaline (ISO) was used to stimulate the hypertrophic response and mitochondrial fragmentation in cultured cardiomyocytes and . We performed immunoblotting, immunofluorescence, and quantitative real-time PCR to validate the function of Mfn1 in cardiomyocyte hypertrophy. Bioinformatic analyses, a luciferase reporter assay, and gain- and loss-of-function studies were used to demonstrate the biological function of miR-153-3p, which regulates mitochondrial fragmentation and hypertrophy by targeting Mfn1. Moreover, ChIP-qPCR and a luciferase reporter assay were performed to identify transcription factor NFATc3 as an upstream regulator to control the expression of miR-153-3p. Our results show that ISO promoted mitochondrial fission and enhanced the expression of miR-153-3p in cardiomyocytes. Knockdown of miR-153-3p attenuated ISO-induced mitochondrial fission and hypertrophy in cultured primary cardiomyocytes. miR-153-3p suppression inhibited mitochondrial fragmentation in ISO-induced cardiac hypertrophy in a mouse model. We identified direct targeting of Mfn1, a key protein of the mitochondrial fusion process, by miR-153-3p. Also, miR-153-3p promoted ISO-induced mitochondrial fission by suppressing the translation of Mfn1. We further found that NFATc3 activated miR-153-3p expression. Knockdown of NFATc3 inhibited miR-153-3p expression and blocked mitochondrial fission and hypertrophic response in cardiomyocytes. Our data revealed a novel signaling pathway, involving NFATc3, miR-153-3p, and Mfn1, which could be a therapeutic target for the prevention and treatment of cardiac hypertrophy.
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http://dx.doi.org/10.7150/thno.37181DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6929994PMC
April 2021

An Exceptionally Stable Tb-Based Metal-Organic Framework for Selectively and Sensitively Detecting Antibiotics in Aqueous Solution.

Inorg Chem 2019 Jun 29;58(12):7746-7753. Epub 2019 May 29.

Faculty of Chemistry , Northeast Normal University , Changchun 130024 , People's Republic of China.

An exceptionally stable metal-organic framework based on one-dimensional (1D) Tb chains with significant green emission under excitation energy, {[Tb(TATMA)(HO)·2HO} (namely, 1), has been fabricated successfully under hydrothermal conditions. By virtue of the spectral overlap between the absorbance spectra of nitrofurans (NFAs) and the excitation spectrum of MOF 1, the resultant sample exhibits outstandingly sensitive and selective luminescence detectability for NFT ( K = 3.35 × 10 M) and NFZ ( K = 3.00 × 10 M) by quenching phenomenon. More importantly, it can detect NFAs in water from bovine serum samples. The portable MOF film can be easily prepared and used with excellent stability and recursitivity.
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http://dx.doi.org/10.1021/acs.inorgchem.9b00147DOI Listing
June 2019

A Versatile Microporous Zinc(II) Metal-Organic Framework for Selective Gas Adsorption, Cooperative Catalysis, and Luminescent Sensing.

Inorg Chem 2018 Jun 7;57(12):7314-7320. Epub 2018 Jun 7.

State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of Chemistry , Jilin University , Changchun 130012 , People's Republic of China.

A versatile microporous zinc(II) MOF (1) with plentiful Lewis basic sites and open metal sites synchronously has been synthesized successfully. The resultant microporous material displays excellent selectivity adsorption for small gases. The IAST selectivity values for CO/CH, CH/CH, CO/N, and CH/CH are 7, 21, 38, and 125 at 101 kPa and 298 K, respectively. 1 exhibits remarkably heterogeneous catalytic performance for acid-base one-pot reactions. Furthermore, 1 exhibits recyclable selective detectability for TNP. The results illustrate that 1 is a versatile material for selective gas adsorption, cooperative catalysis, and luminescent sensing.
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http://dx.doi.org/10.1021/acs.inorgchem.8b00938DOI Listing
June 2018

A micrometer-sized europium(iii)-organic framework for selective sensing of the CrO anion and picric acid in water systems.

Dalton Trans 2017 Oct;46(39):13502-13509

Key Laboratory of Inorganic-Organic Hybrid Functional Material Chemistry, Ministry of Education, Tianjin Key Laboratory of Structure and Performance for Functional Molecules, College of Chemistry, Tianjin Normal University, Tianjin 300387, P. R. China.

A micrometer-sized europium(iii)-organic framework with asymmetric binuclear metal subunits extended by 4,5-dichlorophthalaten (DCPA), [Eu(HO)(DCPA)], was easily obtained using a reverse microemulsion method. The framework exhibits good dispersibility, excellent thermal and environmental stability and easy regeneration ability. More importantly, the complex displays strong red emission and can selectively and sensitively detect both inorganic CrO anions (K = 8.7 × 10 M) and organic picric acid contaminants (K = 1.07 × 10 M) in water systems through fluorescence quenching. A luminescent film of 1 was further prepared and successfully used to detect the CrO anion in an aqueous system. These interesting results indicate that the well-dispersed europium(iii)-organic framework can serve as a promising dual-responsive luminescent sensor for environmental pollutant monitoring.
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http://dx.doi.org/10.1039/c7dt02531aDOI Listing
October 2017

[Effects of nitrogen forms on the growth, yield and fruit quality of tomato under controlled alternate partial root zone irrigation].

Ying Yong Sheng Tai Xue Bao 2014 Dec;25(12):3547-55

The effects of nitrogen (N) forms (ammonium-N and nitrate-N) on the growth, yield and fruit quality of tomato plants (cv. Zhongyan 988) under controlled alternate partial root zone irrigation (APRI) were examined in a split-root experiment. Under the same irrigation mode and/or controlled soil water limitation treatment, ammonium-N promoted plant growth at the early stage, while nitrate-N improved plant growth and development at the later stage leading to higher biomass accumulation and fruit yield at harvest. Under APRI and the same soil water conditions, plants of the nitrate-N treatment improved the content of vitamin C and the ratio of soluble sugar to organic acid and thus facilitated fruit quality when compared with those of the ammonium-N treatment. Plant height and leaf area under APRI treatment were lower compared with conventional irrigation (CK) under the same N form, but the stem diameter under APRI treatment with 60% theta(f) (field water capacity, theta(f)) soil moisture showed a slight increase at the late growth stage. Under the same N form, fruit yield was significantly lower in APRI treatment than that of the CK. Compared with the CK, fruit yield decreased by 22.4%-26.3% under the APRI treatment with 40% theta(f) soil moisture. Under 60% theta(f) soil moisture, the APRI treatment significantly improved fruit quality and water-use efficiency compared with the CK regardless small reduction (5.3%-5.4%) in fruit yield. The experimental results suggested that the APRI treatment with the lower limitation of soil moisture controlled at 60% theta(f), and nitrate-N supply would be the optimal option in terms of sustainable use of water resource and fertilizer.
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December 2014

Correlation research on ADMA plasma levels and left ventricular function of peritoneal dialysis patients.

Int J Clin Exp Med 2014 15;7(11):4455-60. Epub 2014 Nov 15.

Department of Nephrology, The Affiliated Hospital of Inner Mongolia Medical University No. 1 Tongdaobei Street, Hohhot City 010059, Inner Mongolia, P. R. China.

Asymmetric dimethylarginine (ADMA) has been involved in the development mechanism of cardiovascular disease (CVD) in patients with chronic kidney disease. The aim of this study is to investigate the relationship between the plasma ADMA levels and echocardiography, and understand the relationship between ADMA and left ventricular function. All of the patients were divided into three groups, including End-stage renal disease patients on CAPD, Conservative treatment in patients with ESRD and Control group. All the cases in the outpatient clinic or hospital at the next morning were collected fasting venous blood 2 ml. All cases were detected by American GE company Vivid7 Colour Doppler Ultrasonic Echocardiograph to detected left ventricular end-diastolic dimension (LVEDD), Left atrial diameter (LAD), Left ventricular posterior wall thickness in diastole (LVPWT), Interventricular septum thickness in diastole (IVST), left ventricular ejection fraction (LVEF). There were significant differences among all of the three groups for the GFR, urine albumin, SGA, Hb, iPTH and ALB levels. There was statistically significant difference for serum ADMA levels among three groups (F = 34.047, P = 0.000). CAPD patient plasma ADMA levels were negatively correlated with LVEF, and positively correlated with LVMI, LVM, LVEDD, LAD. Conservative treatment group had higher proportion of average artery, left ventricular hypertrophy and left ventricular mass index. The peritoneal dialysis fluid ADMA levels of CAPD patients with peritoneal were positively correlated with LVEF (r = 0.367, P = 0.046), negatively correlated with LVMI. In conclusion, ADMA may be involved in change of left ventricular structure, function, and remodeling process through a complex network.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4276226PMC
December 2014

[Expression of Bcl-2 and Bax proteins in myocardial cells after Macleaya cordata alkaloids poisoning in rats].

Fa Yi Xue Za Zhi 2009 Aug;25(4):246-8, 253

Institute of Forensic Science, Gongli Hospital of Pudong New District, Shanghai 200135, China.

Objective: To observe the expression of Bcl-2 and Bax proteins in rat's myocardial cells after Macleaya cordata alkaloids poisoning, and to provide certain molecular biology references for the detection of Macleaya cordata alkaloids poisoning.

Methods: Experimental model of Macleaya cordata alkaloids poisoning was established, the expression levels of Bcl-2 and Bax proteins in these cells were detected by immunohistochemistry, and the results were analyzed by computer image system.

Results: The expression levels of Bcl-2 and Bax proteins in myocardial cells in poisoning groups were much greater than those in the control groups (P<0.05).

Conclusion: If the clinical symptoms may not be obvious, the detection of Bcl-2 and Bax proteins level by immunohistochemistry still could be ancillary method.
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August 2009

[Application of spiral CT 3D reconstruction of rib fracture in clinical forensic practice].

Fa Yi Xue Za Zhi 2007 Dec;23(6):431-3, 437

Institute of Forensic Science, Gongli Hospital, Pudong New District, Shanghai, China.

Objective: To assess the clinical application of spiral CT 3D reconstruction in forensic diagnosis of rib fracture.

Methods: Thirty-five cases of rib fractures were collectively studied by X-ray, regular CT scan, and adoptive 3D reconstruction with spiral CT.

Results: Clear and stereoscopic images of rib fractures without interference of the surrounding soft tissue were obtained in all of 35 cases examined.

Conclusion: Spiral CT 3D reconstruction could be a valuable means in forensic diagnosis of rib fracture.
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December 2007

[Apoptosis in myocardial cells after macleaya cordata total alkaloids poisoning in rats].

Fa Yi Xue Za Zhi 2006 Oct;22(5):330-2

Department of forensic medicine, Wannan medical college, Wuhu 241001, China.

Objective: To observe pathological changes and apoptosis in rats myocardial cells after Macleaya cordata total alkaloids poisoning, and to provide some references for Macleaya cordata total alkaloids poisoning detection.

Methods: An experimental model of Macleaya cordata total alkaloids poisoning was established, and the technology of TUNEL staining was used.The results were analyzed by computer image analysis competitive system.

Results: Quantities of apoptosis in myocardial cells in poisoning groups were much more than those in the control groups at different tages (P<0.01). In addition the quantities of apoptosis were different after different poisoning duration.

Conclusion: Although clinical symptoms was not obvious and could not be detected by poison analysis. Pathological changes induced by Macleaya cordata total alkaloids could be found through the apoptosis detection.
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October 2006

[Effects of renal artery stenting on renal function of patients with ischaemic nephropathy].

Zhonghua Yi Xue Za Zhi 2005 Aug;85(29):2046-9

Hypertension Division, Cardiovascular Institute, Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100037, China.

Objectives: To evaluate the effects of stent revascularization on renal function of patients with atherosclerotic renal artery stenosis with renal insufficiency.

Methods: Percutaneous transluminal renal angioplasty with stent (PTRAS) was performed on 27 consecutive patients with severe atherosclerotic renal artery stenosis for preservation of renal function, and follow-up was conducted for 6 approximately 48 months.

Results: Technically PTRAS was successfully performed in all 27 patients. The creatinine value was 155 micromol/L +/- 31 micromol/L before operation, and increased to 189 micromol/L +/- 38 micromol/L (P < 0.001), then decreased to 145 micrommol/L +/- 22 micrommol/L, 143 micromol/L +/- 22 micromol/L, 143 micromol/L +/- 24 micromol/L, and 139 micromol/L +/- 10 micromol/L, 6, 12, 24, and 36 months after the PTRAS respectively (all P < 0.05). The severe complications related to the procedure mainly included 7 cases of acute renal failure, 4 cases being reversible. One case died of cardiac infarction with underlying coronary heart disease 9 months after and 1 case died heart failure due to valvular disease of heart 25 months after.

Conclusion: Renal artery stent revascularization is effective for preservation of renal function in patients with ischaemic nephropathy effect on. However, complications related to the procedure frequently occurred. The long-term efficacy is to be investigated further.
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August 2005
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