Publications by authors named "Dawei Yang"

152 Publications

c-Myc G-quadruplex is sensitively and specifically recognized by a fluorescent probe.

Talanta 2021 May 21;226:122125. Epub 2021 Jan 21.

Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry Chinese Academy of Sciences, Beijing, 100190, PR China. Electronic address:

The G-quadruplex structure formed by the c-myc gene sequence has attracted much attention due to its important physiological function in biology and wide application in nanotechnology. So far, probes capable of recognition of c-myc G-quadruplex with both high specificity and sensitivity are still scarce. This work presented a cyanine dye fluorescent probe named Cy-1, which has almost no fluorescence in aqueous solution, but showing more than 1000-fold fluorescence enhancement for recognizing c-myc G-quadruplex. Cy-1 also has good specificity and can selectively recognize c-myc G-quadruplex from other a variety of G-quadruplex and non-G-quadruplex structures. These properties make Cy-1 a promising probe for c-myc G-quadruplex recognition in nanotechnology or biology.
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http://dx.doi.org/10.1016/j.talanta.2021.122125DOI Listing
May 2021

Hyperglycemia accelerates inflammaging in the gingival epithelium through inflammasomes activation.

J Periodontal Res 2021 Mar 2. Epub 2021 Mar 2.

State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.

Background And Objective: Diabetes accelerates inflammaging in various tissue with an increase in senescent cell burden and senescence-associated secretory phenotype (SASP) secretion, which is a significant cause of tissue dysfunction and contributes to the diabetic complications. Recently, inflammasomes are thought to contribute to inflammaging. Here, utilizing diabetic models in vivo and in vitro, we investigated the potential association between hyperglycemia-induced inflammaging and gingival tissue dysfunction and the mechanism underlying inflammasome-associated inflammaging.

Materials And Methods: Gingival epithelium and serum were collected from control and diabetic patients and mice. The expression of p16, p21, and inflammasomes in the gingival epithelium, SASP factors in serum, and the molecular factors associated with gingival epithelial barrier function were assessed. Human oral keratinocyte (HOK) was stimulated with normal and high glucose, and pre-treated with Z-YVAD-FMK (Caspase-1 inhibitor) prior to evaluating cellular senescence, SASP secretion, and inflammasome activation.

Results: In vivo, hyperglycemia significantly elevated the local burden of senescent cells in the gingival epithelium and SASP factors in the serum and simultaneously reduced the expression levels of Claudin-1, E-cadherin, and Connexin 43 in the gingival epithelium. Interestingly, the inflammasomes were activated in the gingival epithelium. In vitro, high glucose-induced the inflammaging in HOK, and blocking inflammasome activation through inhibiting Caspase-1 and glucose-induced inflammaging.

Conclusions: Hyperglycemia accelerated inflammaging in the gingival epithelium through inflammasomes activation, which is potentially affiliated with a decline in the gingival epithelial barrier function in diabetes. Inflammasomes-related inflammaging may be the crucial mechanism underlying diabetic periodontitis and represents significant opportunities for advancing prevention and treatment options.
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http://dx.doi.org/10.1111/jre.12863DOI Listing
March 2021

Identification of GL-V9 as a novel senolytic agent against senescent breast cancer cells.

Life Sci 2021 May 19;272:119196. Epub 2021 Feb 19.

State Key Laboratory of Natural Medicines, Jiangsu Key Laboratory of Carcinogenesis and Intervention, Department of Physiology, School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing 210009, People's Republic of China. Electronic address:

Senescent cancer cells contribute to tumor refractoriness. The removal of senescent cells after chemotherapy prevents or delays cancer relapse. Our study showed that GL-V9 (5-hydroxy-8-methoxy-2-phenyl-7-(4-(pyrrolidin-1-yl) butoxy)-4-H-chromen-4-one), a potential anticancer drug, eliminated senescent MEFs (Mouse embryonic fibroblasts) and drug-induced senescent breast cancer cells. GL-V9 induced apoptosis in senescent MDA-MB-231 cells. Mechanistically, it alkalized lysosomes and increased the abundance of mitochondria as well as ROS (Reactive oxygen species). The senolytic effect of GL-V9 was also observed in epirubicin-treated mammary tumors in MMTV-PyMT mice. Our data thus indicated that GL-V9 is a promising senolytic drug which could be used to improve the outcome of cancer chemotherapy.
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http://dx.doi.org/10.1016/j.lfs.2021.119196DOI Listing
May 2021

Vascular Notch Signaling in Stress Hematopoiesis.

Front Cell Dev Biol 2020 21;8:606448. Epub 2021 Jan 21.

Division of Pulmonary, Critical Care, and Sleep Medicine, Fibrosis Research Center, Icahn School of Medicine at Mount Sinai, Mount Sinai-National Jewish Respiratory Institute, New York, NY, United States.

Canonical Notch signaling is one of the most conserved signaling cascades. It regulates cell proliferation, cell differentiation, and cell fate maintenance in a variety of biological systems during development and cancer (Fortini, 2009; Kopan and Ilagan, 2009; Andersson et al., 2011; Ntziachristos et al., 2014). For the hematopoietic system, during embryonic development, Notch1 is essential for the emergence of hematopoietic stem cells (HSCs) at the aorta-gornado-mesonephro regions of the dorsal aorta. At adult stage, Notch receptors and Notch targets are expressed at different levels in diverse hematopoietic cell types and influence lineage choices. For example, Notch specifies T cell lineage over B cells. However, there has been a long-lasting debate on whether Notch signaling is required for the maintenance of adult HSCs, utilizing transgenic animals inactivating different components of the Notch signaling pathway in HSCs or niche cells. The aims of the current mini-review are to summarize the evidence that disapproves or supports such hypothesis and point at imperative questions waiting to be addressed; hence, some of the seemingly contradictory findings could be reconciled. We need to better delineate the Notch signaling events using biochemical assays to identify direct Notch targets within HSCs or niche cells in specific biological context. More importantly, we call for more elaborate studies that pertain to whether niche cell type (vascular endothelial cells or other stromal cell)-specific Notch ligands regulate the differentiation of T cells in solid tumors during the progression of T-lymphoblastic lymphoma (T-ALL) or chronic myelomonocytic leukemia (CMML). We believe that the investigation of vascular endothelial cells' or other stromal cell types' interaction with hematopoietic cells during homeostasis and stress can offer insights toward specific and effective Notch-related therapeutics.
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http://dx.doi.org/10.3389/fcell.2020.606448DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7873850PMC
January 2021

Automatic prediction of treatment outcomes in patients with diabetic macular edema using ensemble machine learning.

Ann Transl Med 2021 Jan;9(1):43

Guangdong Eye Institute, Department of Ophthalmology, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, The Second School of Clinical Medicine, Southern Medical University, Guangzhou, China.

Background: This study aimed to predict the treatment outcomes in patients with diabetic macular edema (DME) after 3 monthly anti-vascular endothelial growth factor (VEGF) injections using machine learning (ML) based on pretreatment optical coherence tomography (OCT) images and clinical variables.

Methods: An ensemble ML system consisting of four deep learning (DL) models and five classical machine learning (CML) models was developed to predict the posttreatment central foveal thickness (CFT) and the best-corrected visual acuity (BCVA). A total of 363 OCT images and 7,587 clinical data records from 363 eyes were included in the training set (304 eyes) and external validation set (59 eyes). The DL models were trained using the OCT images, and the CML models were trained using the OCT images features and clinical variables. The predictive posttreatment CFT and BCVA values were compared with true outcomes obtained from the medical records.

Results: For CFT prediction, the mean absolute error (MAE), root mean square error (RMSE), and R of the best-performing model in the training set was 66.59, 93.73, and 0.71, respectively, with an area under receiver operating characteristic curve (AUC) of 0.90 for distinguishing the eyes with good anatomical response. The MAE, RMSE, and R was 68.08, 97.63, and 0.74, respectively, with an AUC of 0.94 in the external validation set. For BCVA prediction, the MAE, RMSE, and R of the best-performing model in the training set was 0.19, 0.29, and 0.60, respectively, with an AUC of 0.80 for distinguishing eyes with a good functional response. The external validation achieved a MAE, RMSE, and R of 0.13, 0.20, and 0.68, respectively, with an AUC of 0.81.

Conclusions: Our ensemble ML system accurately predicted posttreatment CFT and BCVA after anti-VEGF injections in DME patients, and can be used to prospectively assess the efficacy of anti-VEGF therapy in DME patients.
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http://dx.doi.org/10.21037/atm-20-1431DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7859823PMC
January 2021

Hydrogen sulfide inhibits endoplasmic reticulum stress through the GRP78/mTOR pathway in rat chondrocytes subjected to oxidative stress.

Int J Mol Med 2021 04 4;47(4). Epub 2021 Feb 4.

Department of Orthopedic Surgery, The Fourth Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China.

The activation of oxidative stress is a primary cause of chondrocyte apoptosis in osteoarthritis (OA). The 78‑kDa glucose‑regulated protein (GRP78)/mammalian target of rapamycin (mTOR) signaling pathway has been demonstrated to be linked with the endoplasmic reticulum (ER) and autophagy. Hydrogen sulfide (HS) has been reported to exert antioxidant effects. The present study investigated oxidative stress levels via 2',7'‑dichlorofluorescin diacetate and MitoSOX staining, apoptosis rates via flow cytometry and the expression levels of ER stress‑related proteins in GYY4137 (donor of HS)‑treated chondrocytes (CHs). CHs were isolated from the bilateral hip joints of male rats to examine mitochondrial permeability transition pore opening‑ and mTOR signaling pathway‑related proteins. The results demonstrated that tert‑Butyl hydroperoxide (TBHP) increased CH apoptosis, and treatment with GYY4137 ameliorated TBHP‑mediated the generation of ROS and CH apoptosis. Moreover, TBHP‑treated CHs displayed elevated ER stress sensor expression levels and apoptotic rates; however, the TBHP‑induced protein expression levels were decreased following GYY4137 treatment. In the present study, treatment with either GYY4137 or transfection with GRP78 siRNA both suppressed the activation of p‑P70S6k and p‑mTOR. HS played an important role in regulating ER stress in TBHP‑stimulated CHs. GYY4137 promoted autophagy, which was accompanied by the inhibition of ER stress. On the whole, the present study demonstrates that TBHP‑induced oxidative stress stimulates ER interactions and CH apoptosis, which are suppressed by exogenous HS via modulating the GRP78/mTOR signaling pathway.
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http://dx.doi.org/10.3892/ijmm.2021.4867DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7891823PMC
April 2021

Prevalence and risk analysis of mobile colistin resistance and extended-spectrum -lactamase genes carriage in pet dogs and their owners: a population based cross-sectional study.

Emerg Microbes Infect 2021 Dec;10(1):242-251

Department of Clinical Veterinary Medicine, College of Veterinary Medicine, China Agricultural University, Beijing, People's Republic of China.

Mobile colistin resistance gene and extended-spectrum -lactamase gene are highly prevalent in human - and pet-derived bacteria. Isolation of identical strains of -positive (MCRPEC) or -positive (CTX-MPEC) from pets and humans highlighted the potential for co-colonization of antibiotic-resistant bacteria which can be a risk for dissemination of resistance genes. In this study, the prevalence of and carriage from rectal swabs in 299 families (dogs and their owners) were 2.7 and 5.3%, respectively. We identified a significant association of carriage between dogs and their owners. Whilst antibiotic use in the previous three months was associated with carriage in dogs. Only one instance of dog and owner carrying identical CTX-MPEC was observed. Although the prevalence of identical strains in one family is rare, the huge number of dog ownership worldwide suggest that this threat should not be underestimated.
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http://dx.doi.org/10.1080/22221751.2021.1882884DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7889244PMC
December 2021

Abundance of tigecycline resistance genes and association with antibiotic residues in Chinese livestock farms.

J Hazard Mater 2021 May 6;409:124921. Epub 2021 Jan 6.

Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China. Electronic address:

The discovery of plasmid-mediated tet(X) variants and efflux pump gene tmexCD1-toprJ1 conferring bacteria resistance to tigecycline has compromised glycylcycline as the last line of defense against infection, which poses serious threat to public health. Herein, real-time quantitative PCR was used to detect the abundance of seven tigecycline resistance genes (TRGs), including six tet(X) variants and tmexCD1-toprJ1, and insertion sequences ISCR2 and IS26. Then, the concentrations of nine antibiotics were quantified in fecal samples collected from 157 livestock farms in four Chinese provinces. TRGs, especially tet(X4), tmexCD1-toprJ1, and insertion sequences ISCR2 and IS26, were more abundant in chicken feces than in pig and cattle feces, suggesting the greater risk for the propagation of TRGs in chicken feces. Positive correlations (ρ = 0.3741-0.8275, P < 0.0001) between ISCR2/IS26 and TRGs (except tet(X1)) further demonstrated that ISCR2 mediates the transfer of tet(X3), tet(X4), and tet(X5) and that IS26 plays a certain role for the mobilization of tet(X4) and tmexCD1-toprJ1. Tetracyclines had no positive correlation with the abundance of TRGs (except tet(X1)), meanwhile florfenicol and tiamulin were positively correlated with TRGs. However, further research is needed to confirm whether or not florfenicol and tiamulin are potential driving factors of TRG accumulation.
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http://dx.doi.org/10.1016/j.jhazmat.2020.124921DOI Listing
May 2021

Effect of breath holding at the end of the inspiration and expiration phases on liver stiffness measured by 2D-MR elastography.

Abdom Radiol (NY) 2021 Jan 2. Epub 2021 Jan 2.

Department of Radiology, Beijing Friendship Hospital, Capital Medical University, No. 95 Yongan Road, West District, Beijing, 100050, China.

Purpose: Evaluate the effect of breath holding at the end of the inspiration and expiration phases on the measurement of liver stiffness by 2D-MR elastography (MRE).

Methods: The study included 61 subjects, of which 31 subjects were pathologically confirmed liver fibrosis patients [liver fibrosis group (LFG)] and 30 healthy subjects [healthy group (HG)]. MRE was used to measure the liver stiffness of subjects in inspiration breath-hold (IBH) and expiration breath-hold (EBH) states.

Results: In IBH and EBH states, the liver stiffness measured by MRE was not significantly different in the HG (P = 0.125, > 0.05), while the LFG showed a significant difference (P <0.001). Also, a significant difference was observed between the change values in the mild/moderate fibrosis group (MFG) and advanced fibrosis group (AFG) (P = 0.005, < 0.05).

Conclusions: The liver stiffness values in patients with liver fibrosis were affected by breath-holding states. The higher the stage of liver fibrosis, the greater the change in liver stiffness values, but no significant difference was observed between liver stiffness values in healthy subjects under the two breath-holding conditions. Different breath-holding states are factors influencing liver fibrosis stiffness measured by MRE, which should be given due attention in both clinical and research contexts.
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http://dx.doi.org/10.1007/s00261-020-02893-wDOI Listing
January 2021

Prevalence and antimicrobial susceptibility of Clostridium perfringens in chickens and pigs from Beijing and Shanxi, China.

Vet Microbiol 2020 Nov 24;252:108932. Epub 2020 Nov 24.

Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, College of Veterinary Medicine, China Agricultural University, Beijing, China. Electronic address:

The prevalence and antimicrobial susceptibility of Clostridium perfringens (C. perfringens) in chickens and pigs were investigated in Beijing and Shanxi, China. In total, 322 C. perfringens (chicken n = 60 and pig n = 262) were obtained from 620 feces of chickens (n = 256) and pigs (n = 364). Multiplex PCR for toxin typing of C. perfringens revealed that all the isolates belong to type A, with 45.7 % (147/322) isolates carrying beta-2 toxin-encoding gene cpb2. Minimum inhibitory concentrations of 27 antimicrobial agents showed that 91.0 % of the tested C. perfringens isolates were resistant to gentamicin and sulfonamides (sulfisoxazole and trimethoprim-sulfamethoxazole), and little resistance was showed to amoxicillin-clavulanate, ceftiofur, doxycycline, vancomycin and linezolid. Additionally, nosiheptide, avilamycin, virginiamycin and bacitracin exhibited good activity against the tested C. perfringens with low MIC (0.06 to ≤4 μg/mL) and MIC values (0.25-8 μg/mL). Whole genome sequencing (WGS) of 48 representative isolates from each farm indicated that the C. perfringens contained diverse antimicrobial resistance genes [tetA(P), ant(6)-Ib, erm(Q), etc.] and toxin genes (cpb2, colA, cloSI, pfoA, etc.). By comparative analysis, four C. perfringens isolates from three different pig farms harboured cpb2-carrying plasmid p1 with 100 % nucleotide sequence identity, suggesting horizontal gene transfer among these microorganisms. The further phylogenomic reconstruction, based on the core-genome single nucleotide polymorphisms (SNPs) of the representatives, demonstrating that C. perfringens from the same farms and regions were closely related. These findings expanded our knowledge of C. perfringens isolated from animals in China, which provided scientific basis for efficient intervention or prevention measures of antimicrobial resistance in animal husbandry in China.
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http://dx.doi.org/10.1016/j.vetmic.2020.108932DOI Listing
November 2020

High-throughput single-EV liquid biopsy: Rapid, simultaneous, and multiplexed detection of nucleic acids, proteins, and their combinations.

Sci Adv 2020 Nov 20;6(47). Epub 2020 Nov 20.

Department of Pulmonary Medicine, Shanghai Respiratory Research Institute, Zhongshan Hospital, Fudan University, Shanghai, China.

MicroRNAs (miRNAs), mRNA, and proteins in/on extracellular vesicles (EVs) represent potential cancer biomarkers. Concurrent detection of multiple biomarkers at a single-EV level would greatly improve prognosis and/or diagnosis and understanding of EV phenotypes, biogenesis, and functions. Here, we introduced a High-throughput Nano-bio Chip Integrated System for Liquid Biopsy (HNCIB) system for simultaneous detection of proteins and mRNA/miRNA in a single EV. Validated through systematic control experiments, HNCIB showed high reliability, sensitivity, and specificity. In a panel of 34 patients with lung adenocarcinoma (LUAD) and 35 healthy donors, HNCIB detected an up-regulated expression of programmed death-ligand 1 mRNA and protein and miR-21 in EVs derived from patients with LUAD compared to those from healthy donors. HNCIB has low sample requirement (~90 μl), fast assay time (~6 hours), and high throughput (up to 384 samples per assay) and would have great potential in the study of EVs and their clinical applications.
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http://dx.doi.org/10.1126/sciadv.abc1204DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7679165PMC
November 2020

Novel Biocompatible Zr-Based Alloy with Low Young's Modulus and Magnetic Susceptibility for Biomedical Implants.

Materials (Basel) 2020 Nov 13;13(22). Epub 2020 Nov 13.

School of Material Science and Engineering, Central South University, Changsha 410083, China.

The microstructure, mechanical properties, magnetic susceptibility, electrochemical corrosion performance, in vitro cell compatibility and blood consistency of Zr-16Nb-xTi (x = 0, 4, 8, 12 and 16 wt.%) materials were investigated as potential materials for biomedical implants. X-ray diffraction (XRD) and Transmission electron microscopy (TEM) analyses revealed the secondary phase martensite α' formed during the quenching process. The phase composition contained metastable β and martensite α', resulting from Ti addition. These phase constitutions were the main causes of a low Young's modulus and magnetic susceptibility. The in vitro cytocompatibility analysis illustrated that the MG63 cells maintained high activity (from 91% to 97%) after culturing in Zr-16Nb-xTi extraction media for 12 days due to the high internal biocompatibility of Zr, Nb and Ti elements, as well as the optimal corrosion resistance of Zr-16Nb-xTi. On the basis of Inductively coupled plasma optical emission spectrometry (ICP-OES) ion release studies, the concentration of Zr, Nb and Ti was noted to reach the equipment detective limit of 0.001 mg/L, which was much lower than pure Ti. With respect to the corrosion behavior in Hank's solution, Zr-16Nb-16Ti displayed superior properties, possessing the lowest corrosion current density and widest passivation region, attributed to the addition of Ti. The blood compatibility test illustrated that the Zr-16Nb-xTi materials were nonhemolytic, and the platelets maintained a spherical shape, with no aggregation or activation on Zr-16Nb-xTi. Overall, Ti addition has obvious effects on the developed Zr-16Nb-xTi alloys, and Zr-16Nb-4Ti exhibited low magnetic susceptibility, low modulus, good biocompatibility and proper corrosion properties, demonstrating the potential of use as implant biomaterials.
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http://dx.doi.org/10.3390/ma13225130DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7696516PMC
November 2020

Optical coherence tomography angiography in diabetic retinopathy: an updated review.

Eye (Lond) 2021 Jan 24;35(1):149-161. Epub 2020 Oct 24.

Department of Ophthalmology and Visual Sciences, The Chinese University of Hong Kong, Hong Kong Special Administrative Region, China.

Diabetic retinopathy (DR) is a common microvascular complication of diabetes mellitus. Optical coherence tomography angiography (OCTA) has been developed to visualize the retinal microvasculature and choriocapillaris based on the motion contrast of circulating blood cells. Depth-resolved ability and non-invasive nature of OCTA allow for repeated examinations and visualization of microvasculature at the retinal capillary plexuses and choriocapillaris. OCTA enables quantification of microvascular alterations in the retinal capillary network, in addition to the detection of classical features associated with DR, including microaneurysms, intraretinal microvascular abnormalities, and neovascularization. OCTA has a promising role as an objective tool for quantifying extent of microvascular damage and identify eyes with diabetic macular ischaemia contributed to visual loss. Furthermore, OCTA can identify preclinical microvascular abnormalities preceding the onset of clinically detectable DR. In this review, we focused on the applications of OCTA derived quantitative metrics that are relevant to early detection, staging and progression of DR. Advancement of OCTA technology in clinical research will ultimately lead to enhancement of individualised management of DR and prevention of visual impairment in patients with diabetes.
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http://dx.doi.org/10.1038/s41433-020-01233-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7852654PMC
January 2021

A label-free electrochemical assay for coronavirus IBV H120 strain quantification based on equivalent substitution effect and AuNPs-assisted signal amplification.

Mikrochim Acta 2020 10 23;187(11):624. Epub 2020 Oct 23.

MOE Joint International Research Laboratory of Animal Health and Food Safety, Key Laboratory of Animal Physiology & Biochemistry, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China.

A label-free electrochemical strategy is proposed combining equivalent substitution effect with AuNPs-assisted signal amplification. According to the differences of S1 protein in various infectious bronchitis virus (IBV) strains, a target DNA sequence that can specifically recognize H120 RNA forming a DNA-RNA hybridized double-strand structure has been designed. Then, the residual single-stranded target DNA is hydrolyzed by S1 nuclease. Therefore, the content of target DNA becomes equal to the content of virus RNA. After equivalent coronavirus, the target DNA is separated from DNA-RNA hybridized double strand by heating, which can partly hybridize with probe 2 modified on the electrode surface and probe 1 on AuNPs' surface. Thus, AuNPs are pulled to the surface of the electrode and the abundant DNA on AuNPs' surface could adsorb a large amount of hexaammineruthenium (III) chloride (RuHex) molecules, which produce a remarkably amplified electrochemical response. The voltammetric signal of RuHex with a peak near - 0.28 V vs. Ag/AgCl is used as the signal output. The proposed method shows a detection range of 1.56e to 1.56e μM with the detection limit of 2.96e μM for IBV H120 strain selective quantification detection, exhibiting good accuracy, stability, and simplicity, which shows a great potential for IBV detection in vaccine research and avian infectious bronchitis diagnosis. Graphical abstract.
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http://dx.doi.org/10.1007/s00604-020-04582-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7581468PMC
October 2020

Duck breast muscle proteins, free fatty acids and volatile compounds as affected by curing methods.

Food Chem 2021 Feb 21;338:128138. Epub 2020 Sep 21.

College of Food Science and Technology, Hunan Agricultural University, Changsha, Hunan 410128, China. Electronic address:

The aim of this study was to investigate the effects of different curing methods on protein structure, protein and lipid oxidation, lypolysis and volatile compounds in duck breast meat. The results showed that compared to static brining and pulsed pressure salting, the vacuum tumbling curing significantly decreased the oxidation of proteins and lipids, and the surface hydrophobicity of proteins, increased α-helix structure but decreased the proportion of β-sheet, and increased actomyosin dissociation, liplysis and the free fatty acid content in meat. Meanwhile, vacuum tumbling curing decreased the amount of volatile flavor compounds, hexanal, 2,3-octanone, and off-flavor compounds 1-octen-3-ol and 1-hexanol. This study suggests that concerns on healthiness and the sensory quality of processed meat products should be paid in the selection of curing methods and vacuum tumbling curing is superior in terms of both aspects.
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http://dx.doi.org/10.1016/j.foodchem.2020.128138DOI Listing
February 2021

ZnSe/N-Doped Carbon Nanoreactor with Multiple Adsorption Sites for Stable Lithium-Sulfur Batteries.

ACS Nano 2020 Nov 21;14(11):15492-15504. Epub 2020 Oct 21.

Catalonia Institute for Energy Research-IREC, Sant Adrià de Besòs, Barcelona, 08930, Spain.

To commercially realize the enormous potential of lithium-sulfur batteries (LSBs) several challenges remain to be overcome. At the cathode, the lithium polysulfide (LiPS) shuttle effect must be inhibited and the redox reaction kinetics need to be substantially promoted. In this direction, this work proposes a cathode material based on a transition-metal selenide (TMSe) as both adsorber and catalyst and a hollow nanoreactor architecture: ZnSe/N-doped hollow carbon (ZnSe/NHC). It is here demonstrated both experimentally and by means of density functional theory that this composite provides three key benefits to the LSBs cathode: (i) A highly effective trapping of LiPS due to the combination of sulfiphilic sites of ZnSe, lithiophilic sites of NHC, and the confinement effect of the cage-based structure; (ii) a redox kinetic improvement in part associated with the multiple adsorption sites that facilitate the Li diffusion; and (iii) an easier accommodation of the volume expansion preventing the cathode damage due to the hollow design. As a result, LSB cathodes based on S@ZnSe/NHC are characterized by high initial capacities, superior rate capability, and an excellent stability. Overall, this work not only demonstrates the large potential of TMSe as cathode materials in LSBs but also probes the nanoreactor design to be a highly suitable architecture to enhance cycle stability.
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http://dx.doi.org/10.1021/acsnano.0c06112DOI Listing
November 2020

Synthesis of Amino Acid Schiff Base Nickel (II) Complexes as Potential Anticancer Drugs .

Bioinorg Chem Appl 2020 29;2020:8834859. Epub 2020 Sep 29.

School of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng 252000, China.

Three hexacoordinated octahedral nickel (II) complexes, [Ni (Trp-sal) (phen) (CHOH)] (1), [Ni (Trp--van) (phen) (CHOH)]•2CHOH (2), and [Ni (Trp-naph) (phen) (CHOH)] (3) (where Trp-sal = Schiff base derived from tryptophan and salicylaldehyde, Trp--van = Schiff base derived from tryptophan and -vanillin, Trp-naph = Schiff base derived from tryptophan and 2-hydroxy-1-naphthaldehyde, phen = 1, 10-phenanthroline), have been synthesized and characterized as potential anticancer agents. Details of structural study of these complexes using single-crystal X-ray crystallography showed that distorted octahedral environment around nickel (II) ion has been satisfied by three nitrogen atoms and three oxygen atoms. All these complexes displayed moderate cytotoxicity toward esophageal cancer cell line Eca-109 with the IC values of 23.95 ± 2.54 M for 1, 18.14 ± 2.39 M for 2, and 21.89 ± 3.19 M for 3. Antitumor mechanism studies showed that complex 2 can increase the autophagy, reactive oxygen species (ROS) levels, and decrease the mitochondrial membrane potential remarkably in a dose-dependent manner in the Eca-109 cells. Complex 2 can cause cell cycle arrest in the G2/M phase. Additionally, complex 2 can regulate the Bcl-2 family and autophagy-related proteins.
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http://dx.doi.org/10.1155/2020/8834859DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7542481PMC
September 2020

DETECTION OF MORPHOLOGIC PATTERNS OF DIABETIC MACULAR EDEMA USING A DEEP LEARNING APPROACH BASED ON OPTICAL COHERENCE TOMOGRAPHY IMAGES.

Retina 2020 Oct 1. Epub 2020 Oct 1.

Guangdong Eye Institute, Department of Ophthalmology, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences.

Purpose: To develop a deep learning (DL) model to detect morphologic patterns of diabetic macular edema (DME) based on optical coherence tomography (OCT) images.

Methods: In the training set, 12,365 OCT images were extracted from a public dataset and an ophthalmic center. A total of 656 OCT images were extracted from another ophthalmic center for external validation. The presence or absence of three OCT patterns of DME, including diffused retinal thickening (DRT), cystoid macular edema (CME), and serous retinal detachment (SRD) were labeled with 1 or 0, respectively. A DL model was trained to detect three OCT patterns of DME. Occlusion test was applied for visualization of the DL model.

Results: Applying five-fold cross-validation method in internal validation, the area under the receiver operating characteristic curve (AUC) for detection of three OCT patterns (i.e., DRT, CME, and SRD) were 0.971, 0.974, and 0.994, respectively, with accuracy of 93.0%, 95.1%, and 98.8%, respectively, sensitivity of 93.5%, 94.5%, and 96.7%, respectively, and specificity of 92.3%, 95.6%, and 99.3%, respectively. In external validation, the AUC were 0.970, 0.997, and 0.997, respectively, with accuracy of 90.2%, 95.4%, and 95.9%, respectively, sensitivity of 80.1%, 93.4%, and 94.9%, respectively, and specificity of 97.6%, 97.2%, and 96.5%, respectively. Occlusion test showed that the DL model could successfully identify the pathologic regions most critical for detection.

Conclusions: Our DL model demonstrated high accuracy and transparency in detection of OCT patterns of DME. These results emphasized the potential of artificial intelligence in assisting clinical decision-making processes in DME patients.
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http://dx.doi.org/10.1097/IAE.0000000000002992DOI Listing
October 2020

Updated guidance on the management of COVID-19: from an American Thoracic Society/European Respiratory Society coordinated International Task Force (29 July 2020).

Eur Respir Rev 2020 Sep 5;29(157). Epub 2020 Oct 5.

Dept of Medicine, Boston University School of Medicine, Boston, MA, USA

Background: Coronavirus disease 2019 (COVID-19) is a disease caused by severe acute respiratory syndrome-coronavirus-2. Consensus suggestions can standardise care, thereby improving outcomes and facilitating future research.

Methods: An International Task Force was composed and agreement regarding courses of action was measured using the Convergence of Opinion on Recommendations and Evidence (CORE) process. 70% agreement was necessary to make a consensus suggestion.

Results: The Task Force made consensus suggestions to treat patients with acute COVID-19 pneumonia with remdesivir and dexamethasone but suggested against hydroxychloroquine except in the context of a clinical trial; these are revisions of prior suggestions resulting from the interim publication of several randomised trials. It also suggested that COVID-19 patients with a venous thromboembolic event be treated with therapeutic anticoagulant therapy for 3 months. The Task Force was unable to reach sufficient agreement to yield consensus suggestions for the post-hospital care of COVID-19 survivors. The Task Force fell one vote shy of suggesting routine screening for depression, anxiety and post-traumatic stress disorder.

Conclusions: The Task Force addressed questions related to pharmacotherapy in patients with COVID-19 and the post-hospital care of survivors, yielding several consensus suggestions. Management options for which there is insufficient agreement to formulate a suggestion represent research priorities.
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http://dx.doi.org/10.1183/16000617.0287-2020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7537943PMC
September 2020

Associations between retinal microvasculature/microstructure and renal function in type 2 diabetes patients with early chronic kidney disease.

Diabetes Res Clin Pract 2020 Oct 20;168:108373. Epub 2020 Aug 20.

Department of Ophthalmology, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510000, Guangdong, China. Electronic address:

Aims: To explore the associations between the microvascular/microstructural changes in the retina measured by optical coherence tomography angiography (OCTA) and renal function in type 2 diabetes patients with early chronic kidney disease (CKD).

Methods: This cross-sectional study, including 150 type 2 diabetes patients, was conducted from July 2017 to January 2019. We obtained retinal vessel density (VD) and retinal thickness using OCTA. The correlations between OCTA-derived parameters and CKD-related systemic data were assessed by multiple regression analyses.

Results: We found a significant decrease of VD in patients with CKD. Multiple regression analyses showed that: a) decreased eGFR (estimated glomerular filtration rate) was significantly correlated with decreased VD of superficial vascular complex (SVC) in macular area; b) increased UACR (urine albumin to creatinine ratio) was significantly associated with increased macular thickness; c) decreased HGB/HCT (Hemoglobin or Hematocrit) was significantly correlated with both decreased VD of SVC and increased retinal thickness in macular area.

Conclusions: Decrease in the microcirculation of the retina and thickening of the macula associated with impaired renal function in type 2 diabetes. Our finding encourages the application of OCTA-derived metrics in diabetic eyes to monitor the progression of CKD.
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http://dx.doi.org/10.1016/j.diabres.2020.108373DOI Listing
October 2020

Facile C-N coupling of coordinated ammonia and labile carbonyl or acetonitrile promoted by a thiolate-bridged dicobalt reaction scaffold.

Dalton Trans 2020 Aug 6;49(32):11260-11267. Epub 2020 Aug 6.

State Key Laboratory of Fine Chemicals, Dalian University of Technology, 2 Linggong Road, Dalian, 116024, P. R. China.

At low temperature, interaction of the thiolate-bridged dicobalt carbonyl complex [Cp*Co(i)(μ-SEt)(CO)CoCp*][I] (Cp* = η-CMe) (1) with NH resulted in the C-N coupling of the coordinated CO and amido group that originate from ammonia activation to afford a dicobalt formylamino complex [Cp*Co(μ-SEt)(μ-η:η-O[double bond, length as m-dash]CNH)CoCp*][I] (2). Interestingly, at relatively high temperatures, the labile CO ligand was replaced by NH to give a thiolate-bridged dicobalt ammonia complex [Cp*Co(i)(μ-SEt)(NH)CoCp*][I] (3). Subsequently, in the presence of the dehalogenation reagent AgPF, the CoS scaffold can simultaneously activate NH and MeCN to produce the complex [Cp*Co(MeCN)(μ-SEt)(NH)CoCp*][PF] (4). Furthermore, in the presence of NaOEt, the facile occurrence of the intramolecular cyclization led to the formation of acetamidino-bridged dicobalt complex [Cp*Co(μ-SEt)(μ-η:η-NH(CCH)NH)CoCp*][PF] (5), which may proceed through the nucleophilic attack of amido from NH to coordinated MeCN followed by the hydrogen atom transfer process. In the presence of MeCN, treatment of 5 with HBF released the corresponding [MeC(NH)NH]BF; meanwhile, the [CoS] core structural scaffold remained. In this CoS reaction system, the cooperative activation effect between the two cobalt centers plays an important role for NH activation and functionalization.
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http://dx.doi.org/10.1039/d0dt02133dDOI Listing
August 2020

Wogonin induces cellular senescence in breast cancer via suppressing TXNRD2 expression.

Arch Toxicol 2020 10 15;94(10):3433-3447. Epub 2020 Jul 15.

State Key Laboratory of Natural Medicines, Jiangsu Key Laboratory of Carcinogenesis and Intervention, School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing, 210009, People's Republic of China.

Cellular senescence contributes to tumor regression through both cell autonomous and non-autonomous mechanisms. Drugs inducing cancer cell senescence and modulating senescence-associated secretory phenotype (SASP) render advantage to the cancer treatment. Breast cancer remains the second most cause of female cancer mortality, among which triple-negative breast cancer (TNBC) has a more aggressive clinical course. Our study showed that in TNBC cell lines including MDA-MB-231 and 4T1 cells, moderate concentrations of wogonin (5, 7-dihydroxy-8-methoxy-2-phenyl-4h-1-benzopyran-4-one) (50-100 μM) not only induced permanent proliferation inhibition, but also increased P16 expression, β-galactosidase activity, senescence-associated heterochromatin foci and SASP, which are the typical characteristics of cellular senescence. Moreover, results showed that wogonin-induced senescence was partially attributed to the reactive oxygen species (ROS) accumulation upon wogonin treatment in MDA-MB-231 cells, since elimination of ROS by N-acetylcysteine (NAC) was able to repress wogonin-induced β-galactosidase activity. Mechanistically, wogonin reduced the expression of TXNRD2, an important antioxidant enzyme in controlling the levels of cellular ROS, by altering the histone acetylation at its regulatory region. In addition, senescent MDA-MB-231 cells induced by wogonin exhibited activated NF-κB and suppressed STAT3, which were recognized as regulators of SASP. SASP from these senescent cells suppressed tumor cell growth, promoted macrophage M1 polarization in vitro and increased immune cell infiltration in xenografted tumors in vivo. These results reveal another mechanism for the anti-breast cancer activity of wogonin by inducing cellular senescence, which suppresses tumor progression both autonomously and non-autonomously.
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http://dx.doi.org/10.1007/s00204-020-02842-yDOI Listing
October 2020

Multi-modality self-attention aware deep network for 3D biomedical segmentation.

BMC Med Inform Decis Mak 2020 07 9;20(Suppl 3):119. Epub 2020 Jul 9.

Department of Radiology, Beijing Friendship Hospital, Capital Medical University, Beijing, China.

Background: Deep learning based on segmentation models have been gradually applied in biomedical images and achieved state-of-the-art performance for 3D biomedical segmentation. However, most of existing biomedical segmentation researches take account of the application cases with adapting a single type of medical images from the corresponding examining method. Considering of practical clinic application of the radiology examination for diseases, the multiple image examination methods are normally required for final diagnosis especially in some severe diseases like cancers. Therefore, by considering the cases of employing multi-modal images and exploring the effective multi-modality fusion based on deep networks, we do the research to make full use of complementary information of multi-modal images referring to the clinic experiences of radiologists in image analysis.

Methods: Referring to the human radiologist diagnosis experience, we discuss and propose a new self-attention aware mechanism to improve the segmentation performance by paying the different attention on different modal images and different symptoms. Firstly, we propose a multi-path encoder and decoder deep network for 3D biomedical segmentation. Secondly, to leverage the complementary information among different modalities, we introduce a structure of attention mechanism called the Multi-Modality Self-Attention Aware (MMSA) convolution. Multi-modal images we used in the paper are different modalities of MR scanning images, which are input into the network separately. Then self-attention weight fusion of multi-modal features is performed with our proposed MMSA, which can adaptively adjust the fusion weights according to the learned contribution degree of different modalities and different features revealing the different symptoms from the labeled data.

Results: Experiments have been done on the public competition dataset BRATS-2015. The results show that our proposed method achieves dice scores of 0.8726, 0.6563, 0.8313 for the whole tumor, the tumor core and the enhancing tumor core, respectively. Comparing with the U-Net with SE block, the scores are increased by 0.0212,0.031,0.0304.

Conclusions: We present a multi-modality self-attention aware convolution, which have better segmentation results based on the adaptive weighting fusion mechanism with exploiting the multiple medical image modalities. Experimental results demonstrate the effectiveness of our method and prominent application in the multi-modality fusion based medical image analysis.
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http://dx.doi.org/10.1186/s12911-020-1109-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7346322PMC
July 2020

Detection of the enterococcal oxazolidinone/phenicol resistance gene optrA in Campylobacter coli.

Vet Microbiol 2020 Jul 24;246:108731. Epub 2020 May 24.

Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing, China. Electronic address:

The transferable optrA gene encodes an ABC-F protein which confers resistance to oxazolidinones and phenicols, and has so far been detected exclusively in Gram-positive bacteria, including enterococci, staphylococci and streptococci. Here, we identified for the first time the presence of optrA in naturally occurring Gram-negative bacteria. Seven optrA-positive Campylobacter coli were identified from 563 Campylobacter isolates of animal origin from Guangdong (n = 1, chicken) and Shandong (n = 6, duck) provinces of China in 2017-2018. The detected optrA genes were functionally active and mediated resistance or elevated minimal inhibitory concentrations of linezolid, florfenicol and chloramphenicol in the respective C. coli isolates. The optrA gene, together with other transferable resistance genes, such as fexA, catA9, tet(O), tet(L), erm(A)-like, spc, or aadE, was located in two different chromosome-borne multidrug resistance genomic islands (MDRGIs). In both MDRGIs, complete or truncated copies of the insertion sequence IS1216E were present in the vicinity of optrA. The IS1216E-bracketed genetic environment of optrA was almost identical to the optrA regions on enterococcal plasmids, suggesting that the optrA in Campylobacter probably originated from Enterococcus spp.. Moreover, the formation of an optrA-carrying translocatable unit by recombination of IS1216E indicated that this IS element may play an important role in the horizontal transfer of optrA in Campylobacter. Although optrA was only found in a small number of C. coli isolates, enhanced surveillance is needed to monitor the distribution and the potential emergence of optrA in Campylobacter.
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http://dx.doi.org/10.1016/j.vetmic.2020.108731DOI Listing
July 2020

P53-induced MRVI1 mediates carcinogenesis of colorectal cancer.

Scand J Gastroenterol 2020 Jul 26;55(7):824-833. Epub 2020 Jun 26.

The First Department of General Surgery, The Central People's Hospital of Siping City, Jilin, China.

Colorectal cancer (CRC) is one of the most prevalent cancer types worldwide. Despite the advancements in current diagnosis and treatment strategies of CRC, the incidence and mortality rates of CRC have been rising. To explore novel mechanism of CRC, this study focused on the expression pattern and functional mechanism of murine retrovirus integration site 1 (MRVI1) in CRC. Tumor tissues and adjacent normal tissues were collected from CRC patients, and the expression levels of MRVI1 were determined by RT-PCR and Western blot. MRVI1 knockdown was achieved by shRNA in HCT116 and HT29 cells, followed by CCK-8 assay to detect the cell proliferation, and caspase-3 activity assay combined with nucleosome ELISA assay to detect cell apoptosis. Transwell assay was used to detect cell invasion and luciferase reporter assay was used to validate the activation of the MRVI1 promoter by p53. MRVI1 was downregulated in CRC tissues and several CRC cell lines. Knockdown of MRVI1 enhanced the proliferation and apoptosis, while promoted invasion and stemness of CRC cells. Mechanism study revealed that MRVI1 was transcriptionally activated by p53 at its upstream. In addition, p53-induced inhibition of CRC prognosis depended on MRVI1. MRVI1 inhibited the prognosis of CRC a mechanism involving p53 activation. MRVI1 could serve as a potential target for clinical diagnosis and treatment of CRC.
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http://dx.doi.org/10.1080/00365521.2020.1782465DOI Listing
July 2020

Clinical significance of circulating tumor cells and metabolic signatures in lung cancer after surgical removal.

J Transl Med 2020 06 17;18(1):243. Epub 2020 Jun 17.

Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing, 100700, People's Republic of China.

Background: Lung cancer (LC) remains the deadliest form of cancer globally. While surgery remains the optimal treatment strategy for individuals with early-stage LC, what the metabolic consequences are of such surgical intervention remains uncertain.

Methods: Negative enrichment-fluorescence in situ hybridization (NE-FISH) was used in an effort to detect circulating tumor cells (CTCs) in pre- and post-surgery peripheral blood samples from 51 LC patients. In addition, targeted metabolomics analyses, multivariate statistical analyses, and pathway analyses were used to explore surgery-associated metabolic changes.

Results: LC patients had significantly higher CTC counts relative to healthy controls with 66.67% of LC patients having at least 1 detected CTC before surgery. CTC counts were associated with clinical outcomes following surgery. In a targeted metabolomics analysis, we detected 34 amino acids, 147 lipids, and 24 fatty acids. When comparing LC patients before and after surgery to control patients, metabolic shifts were detected via PLS-DA and pathway analysis. Further surgery-associated metabolic changes were identified when comparing LA (LC patients after surgery) and LB (LC patients before surgery) groups. We identified SM 42:4, Ser, Sar, Gln, and LPC 18:0 for inclusion in a biomarker panel for early-stage LC detection based upon an AUC of 0.965 (95% CI 0.900-1.000). This analysis revealed that SM 42:2, SM 35:1, PC (16:0/14:0), PC (14:0/16:1), Cer (d18:1/24:1), and SM 38:3 may offer diagnostic and prognostic benefits in LC.

Conclusions: These findings suggest that CTC detection and plasma metabolite profiling may be an effective means of diagnosing early-stage LC and identifying patients at risk for disease recurrence.
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http://dx.doi.org/10.1186/s12967-020-02401-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7301449PMC
June 2020

Erratum for Xu et al., "A Single Amino Acid at Position 431 of the PB2 Protein Determines the Virulence of H1N1 Swine Influenza Viruses in Mice".

J Virol 2020 Jun 16;94(13). Epub 2020 Jun 16.

State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, People's Republic of China

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http://dx.doi.org/10.1128/JVI.00653-20DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7307177PMC
June 2020

Aging Suppresses Sphingosine-1-Phosphate Chaperone ApoM in Circulation Resulting in Maladaptive Organ Repair.

Dev Cell 2020 06 15;53(6):677-690.e4. Epub 2020 Jun 15.

Fibrosis Research Center, Mount Sinai-National Jewish Respiratory Institute, Division of Pulmonary, Critical Care, and Sleep Medicine, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Ansary Stem Cell Institute, Division of Regenerative Medicine, Department of Medicine, Weill Cornell Medicine, New York, NY 10065, USA. Electronic address:

Here, we show that the liver-derived apolipoprotein M (ApoM) protects the lung and kidney from pro-fibrotic insults and that this circulating factor is attenuated in aged mice. Aged mouse hepatocytes exhibit transcriptional suppression of ApoM. This leads to reduced sphingosine-1-phosphate (S1P) signaling via the S1P receptor 1 (S1PR1) in the vascular endothelial cells of lung and kidney. Suboptimal S1PR1 angiocrine signaling causes reduced resistance to injury-induced vascular leak and leads to organ fibrosis. Plasma transfusion from Apom transgenic mice but not Apom knockout mice blocked fibrosis in the lung. Similarly, infusion of recombinant therapeutics, ApoM-Fc fusion protein enhanced kidney and lung regeneration and attenuated fibrosis in aged mouse after injury. Furthermore, we identified that aging alters Sirtuin-1-hepatic nuclear factor 4α circuit in hepatocytes to downregulate ApoM. These data reveal an integrative organ adaptation that involves circulating S1P chaperone ApoM high density lipoprotein (HDL), which signals via endothelial niche S1PR1 to spur regeneration over fibrosis.
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http://dx.doi.org/10.1016/j.devcel.2020.05.024DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7607448PMC
June 2020

Circulating tumour cells as prognosis predictive markers of neoadjuvant chemotherapy-treated breast cancer patients.

J Chemother 2020 Oct 5;32(6):304-309. Epub 2020 Jun 5.

Zhong Yuan Academy of Biological Medicine, Liaocheng People's Hospital, Liaocheng, Shandong, P. R. China.

In this study, we detected and measured the count of circulating tumour cells (CTCs) in breast cancer (BC) patients who were treated by neoadjuvant chemotherapy (NAC) in order to assess the clinical validity of CTCs. A total of 96 patients with locally advanced BC and who were treated by NAC were enrolled in this study. The CTC count in the peripheral blood was estimated by negative enrichment-fluorescence hybridization before and after NAC. The clinicopathological data of the patients were recorded. CTCs were detected in 59 of the 96 patients with BC before NAC. Particularly, the detection rate of CTCs was significantly lower in human epidermal growth factor receptor-2 (HER-2)-negative patients than in HER-2-positive patients. CTCs were significantly fewer after NAC than before NAC. The CTC-detection sensitivity in the NAC efficacy evaluation was 75.5% (40/53), while the specificity was 72.1% (31/43). The CTC consistency analysis with clinical effects (Response Evaluation Criteria in Solid Tumors Version 1.1 Standard) was described as moderate (kappa = 0.476,  < 0.001). Thus, our findings suggest that CTC detection is a potential new approach to assess the efficacy of NAC.
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http://dx.doi.org/10.1080/1120009X.2020.1774207DOI Listing
October 2020

Thiolate-Bridged Dicobalt Complexes Bearing Hydrazine, Hydrazido, and Diazenido Ligands: Synthesis, Structural Characterization, and Interconversion.

Inorg Chem 2020 Jun 4;59(12):8203-8212. Epub 2020 Jun 4.

State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024, P. R. China.

Synthetic di- or multimetallic complexes bearing NH nitrogenous ligands in a sulfur-rich coordination environment have attracted considerable attention due to their importance in evaluating the complex mechanism of biological nitrogen fixation. Herein, we report a series of thiolate-bridged dicobalt NH species obtained by treatment of CoCo precursor with hydrazine and its substituted derivatives at ambient temperature. Remarkably, when the substituent is the cyclohexyl group, the resulting species can interconvert through different pathways. This CoS skeleton provides a new model system for obtaining valuable information about the early NH-bound intermediate species during the catalytic cycle of nitrogenase.
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http://dx.doi.org/10.1021/acs.inorgchem.0c00542DOI Listing
June 2020