Publications by authors named "Dawei Wang"

562 Publications

The application of machine learning algorithms in predicting the length of stay following femoral neck fracture.

Int J Med Inform 2021 Sep 13;155:104572. Epub 2021 Sep 13.

International Science and Technology Cooperation Base of Spinal Cord Injury, Tianjin Key Laboratory of Spine and Spinal Cord Injury, Department of Orthopedics, Tianjin Medical University General Hospital, 154 Anshan Road, Heping District, Tianjin, China. Electronic address:

Purpose: Femoral neck fracture is a frequent cause of hospitalization, and length of stay is an important marker of hospital cost and quality of care provided. As an extension of traditional statistical methods, machine learning provides the possibility of accurately predicting the length of hospital stay. The aim of this paper is to retrospectively identify predictive factors of the length of hospital stay (LOS) and predict the postoperative LOS by using machine learning algorithms.

Method: Based on the admission and perioperative data of the patients, linear regression was used to analyze the predictive factors of the LOS. Multiple machine learning models were developed, and the performance of different models was compared.

Result: Stepwise linear regression showed that preoperative calcium level (P = 0.017) and preoperative lymphocyte percentage (P = 0.007), in addition to intraoperative bleeding (p = 0.041), glucose and sodium chloride infusion after surgery (P = 0.019), Charlson Comorbidity Index (p = 0.007) and BMI (P = 0.031), were significant predictors of LOS. The best performing model was the principal component regression (PCR) with an optimal MAE (1.525) and a proportion of prediction error within 3 days of 90.91%.

Conclusion: Excessive intravenous glucose and sodium chloride infusion after surgery, preoperative hypocalcemia, preoperative high percentages of lymphocytes, excessive intraoperative bleeding, lower BMI and higher CCI scores were related to prolonged LOS by using linear regression. Machine learning could accurately predict the postoperative LOS. This information allows hospital administrators to plan reasonable resource allocation to fulfill demand, leading to direct care quality improvement and more reasonable use of scarce resources.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijmedinf.2021.104572DOI Listing
September 2021

LncRNA MATN1-AS1 for Prediction of Prognosis in Osteosarcoma Patients and Its Cellular Function.

Mol Biotechnol 2021 Sep 20. Epub 2021 Sep 20.

Department of Sixth Orthopedics, The 2nd Affiliated Hospital of Harbin Medical University, 246 Xuefu Road, Nangang District, Harbin, 150000, Heilongjiang, China.

Long non-coding RNAs show essential roles in various cancer processes. This study aimed at the expression features, prognosis significance, and biological effect of lnc MATN1-AS1 in osteosarcoma (OS). Five kinds of cell lines and 117 pairs of tissues were analyzed by qRT-PCR for quantification of lnc MATN1-AS1 and miR-1299 level. Clinical data were analyzed using Chi-Square Tests to show the association with lnc MATN1-AS1 level. Kaplan-Meier analysis and Cox regression were used to judge the prognostic value. Cell counting kit-8 and Transwell assay were conducted, respectively, to analyze the effect of lnc MATN1-AS1 on cell proliferation and metastasis. The target miRNA was predicted. lnc MATN1-AS1 level was significantly elevated in OS cells and tissues and related to Enneking staging, lung metastasis, and histologic type. Patients with high lnc MATN1-AS1 level showed a shorter overall survival and recurrence-free survival. Lnc MATN1-AS1 knockdown inhibited OS cell proliferation, migration, and invasion by sponging miR-1299. Lnc MATN1-AS1 has oncogenic features and prognostic significance in OS and is a novel therapeutic strategy for OS.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12033-021-00394-9DOI Listing
September 2021

Global, regional prevalence, incidence and risk factors of knee osteoarthritis in population-based studies.

EClinicalMedicine 2020 Dec 26;29-30:100587. Epub 2020 Nov 26.

Department of Orthopaedics, The Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai 519000, Guangdong, China.

Background: Knee osteoarthritis (OA) is a major cause of disability in the elderly, however, there are few studies to estimate the global prevalence, incidence, and risk factors of knee OA.

Methods: For this study, we searched PUBMED, EMBASE and SCOPUS from inception to April 4, 2020, without language restriction. We identified eligible studies with information on the prevalence or incidence of knee OA in population-based observational studies and extracted data from published reports. We did random-effects meta-analysis to generate estimates. This study was registered with PROSPERO (CRD42020181035).

Findings: Out of 9570 records identified, 88 studies with 10,081,952 participants were eligible for this study. The pooled global prevalence of knee OA was 16⋅0% (95% CI, 14⋅3%-17⋅8%) in individuals aged 15 and over and was 22⋅9% (95% CI, 19⋅8%-26⋅1%) in individuals aged 40 and over. Correspondingly, there are around 654⋅1 (95% CI, 565⋅6-745⋅6) million individuals (40 years and older) with knee OA in 2020 worldwide. The pooled global incidence of knee OA was 203 per 10,000 person-years (95% CI, 106-331) in individuals aged 20 and over. Correspondingly, there are around annual 86⋅7 (95% CI, 45⋅3-141⋅3) million individuals (20 years and older) with incident knee OA in 2020 worldwide. The prevalence and incidence varied substantially between individual countries and increased with age. The ratios of prevalence and incidence in females and males were 1⋅69 (95% CI, 1⋅59-1⋅80, <0⋅00) and 1⋅39 (95% CI, 1⋅24-1⋅56, <0⋅00), respectively.

Interpretation: Our study provides the global prevalence (16⋅0% [95% CI, 14⋅3%-17⋅8%]) and incidence (203 per 10,000 person-years [95% CI, 106-331]) of knee OA. These findings can be used to better assess the global health burden of knee OA. Further prospective cohort studies are warranted to identify modifiable risk factors for providing effectively preventive strategies in the early stages of the disease.

Funding: This work was supported by grants from the National Natural Science Foundation of China (nos. 81772384 and 81572174).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.eclinm.2020.100587DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7704420PMC
December 2020

Biomimetic Mechanoswitchable Interfaces for High-Performance Spatial Gas Bubble Maneuvering.

ACS Appl Mater Interfaces 2021 Sep 3;13(36):43769-43776. Epub 2021 Sep 3.

CAS Key Laboratory of Mechanical Behavior and Design of Materials, Key Laboratory of Precision Scientific Instrumentation of Anhui Higher Education Institutes, Department of Precision Machinery and Precision Instrumentation, University of Science and Technology of China, Hefei 230026, China.

The on-demand manipulation of gas bubbles in aqueous ambient environments is fundamental to many fields such as microfluidics and biochemical microanalysis. However, most bubble manipulation strategies are limited to restricted locomotion on the confined surfaces without spatial convenience of transport. Herein, we report a kind of biomimetic bubble manipulator with mechanoswitchable interfaces (MSIs), featuring the advantages of parallel bubble control and spatial maneuvering flexibility. By the synergic action between Janus aluminum membrane and superaerophilic microfiber array, the gas-MSI interfacial adhesion can be reversibly switched to achieve capturing/releasing underwater bubbles. Moreover, the adhesion force of MSI can be readily tuned by diverse experimental parameters including surface roughness, fiber number, diameter, and spacing of the neighboring microfibers, which are further systematically investigated. Relying on this mobile platform, we demonstrate a series of powerful applications including bubble parallel control, bubble array regrouping, arbitrary bubble transport and even manipulating underwater solids through bubbles, which are otherwise challenging for conventional approaches. We envision that this versatile platform will bring new insights into potential applications, such as cross-species sample control and handheld gas microsyringe.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.1c13527DOI Listing
September 2021

Immune Checkpoints: Therapeutic Targets for Pituitary Tumors.

Dis Markers 2021 16;2021:5300381. Epub 2021 Aug 16.

Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.

Pituitary tumors are the third most common intracranial tumors in adults. Treatment of refractory pituitary tumors is known to be difficult due to limited treatment options. As a promising therapeutic method, tumor immunotherapy has been applied in the treatment of many tumors, including pituitary tumors. Immune checkpoint blocking is one of the effective strategies to activate antitumor immunity. Immune checkpoints prevent tissue damage by regulating the immune response of peripheral tissues and participate in the maintenance of a normal immune environment. In the presence of a tumor, inhibition of T cell activity by tumor cells binding to immune checkpoints and their ligands is an important mechanism for tumor cells to escape immune injury. In this review, we summarize the latest findings of immune checkpoints and their potential as immunotherapeutic targets for pituitary tumors.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2021/5300381DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8384513PMC
August 2021

Pan-Cancer Analysis of Prognostic and Immune Infiltrates for CXCs.

Cancers (Basel) 2021 Aug 18;13(16). Epub 2021 Aug 18.

Department of General Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, China.

Background: CXCs are important genes that regulate inflammation and tumor metastasis. However, the expression level, prognosis value, and immune infiltration of CXCs in cancers are not clear.

Methods: Multiple online datasets were used to analyze the expression, prognosis, and immune regulation of CXCs in this study. Network analysis of the Amadis database and GEO dataset was used to analyze the regulation of intestinal flora on the expression of CXCs. A mouse model was used to verify the fact that intestinal bacterial dysregulation can affect the expression of CXCs.

Results: In the three cancers, multiple datasets verified the fact that the mRNA expression of this family was significantly different; the mRNA levels of CXCL3, 8, 9, 10, 14, and 17 were significantly correlated with the prognosis of three cancers. CXCs were correlated with six types of immuno-infiltrating cells in three cancers. Immunohistochemistry of clinical samples confirmed that the expression of CXCL8 and 10 was higher in three cancer tissues. Animal experiments have shown that intestinal flora dysregulation can affect CXCL8 and 10 expressions.

Conclusion: Our results further elucidate the function of CXCs in cancers and provide new insights into the prognosis and immune infiltration of breast, colon, and pancreatic cancers, and they suggest that intestinal flora may influence disease progression through CXCs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/cancers13164153DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8392715PMC
August 2021

The complete chloroplast genome of .

Mitochondrial DNA B Resour 2021 16;6(9):2650-2651. Epub 2021 Aug 16.

Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China Ministry of Education, Southwest Forestry University, Kunming, China.

Here, we report the complete chloroplast genome of . The genome is 116,940 bp in size, which is comprised of a large single-copy (LSC) region of 74,075 bp, a small single-copy (SSC) region of 40,425 bp, and two short inverted repeat (IR) regions of 1,220 bp. The overall GC content of the plastome was 38.5%. The new sequence comprised 103 unique genes, including 74 protein-coding genes, 4 rRNA genes, and 25 tRNA genes. Phylogenetic analysis showed that was close to and .
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/23802359.2021.1920488DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8381927PMC
August 2021

Transcriptome Analysis of Peripheral Blood Mononuclear Cells Reveals Distinct Immune Response in Asymptomatic and Re-Detectable Positive COVID-19 Patients.

Front Immunol 2021 29;12:716075. Epub 2021 Jul 29.

Department of Laboratory Medicine, Shunde Hospital of Guangzhou University of Chinese Medicine, Foshan, China.

The existence of asymptomatic and re-detectable positive coronavirus disease 2019 (COVID-19) patients presents the disease control challenges of COVID-19. Most studies on immune responses in COVID-19 have focused on moderately or severely symptomatic patients; however, little is known about the immune response in asymptomatic and re-detectable positive (RP) patients. Here we performed a comprehensive analysis of the transcriptomic profiles of peripheral blood mononuclear cells (PBMCs) from 48 COVID-19 patients which included 8 asymptomatic, 13 symptomatic, 15 recovered and 12 RP patients. The weighted gene co-expression network analysis (WGCNA) identified six co-expression modules, of which the turquoise module was positively correlated with the asymptomatic, symptomatic, and recovered COVID-19 patients. The red module positively correlated with symptomatic patients only and the blue and brown modules positively correlated with the RP patients. The analysis by single sample gene set enrichment analysis (ssGSEA) revealed a lower level of IFN response and complement activation in the asymptomatic patients compared with the symptomatic, indicating a weaker immune response of the PBMCs in the asymptomatic patients. In addition, gene set enrichment analysis (GSEA) analysis showed the enrichment of TNFα/NF-κB and influenza infection in the RP patients compared with the recovered patients, indicating a hyper-inflammatory immune response in the PBMC of RP patients. Thus our findings could extend our understanding of host immune response during the progression of COVID-19 disease and assist clinical management and the immunotherapy development for COVID-19.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fimmu.2021.716075DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8359015PMC
August 2021

Ultrafast and stable ion/electron transport of MnNbO in LIC/SC via interface protection and lattice defects.

J Colloid Interface Sci 2021 Aug 5;606(Pt 1):77-86. Epub 2021 Aug 5.

School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, PR China. Electronic address:

Interface protection and kinetics optimization could effectively relieve the shortcomings of bimetallic oxides, such as low conductivity, strong hydrophobicity, insufficient ion diffusion rate and metal interatomic instability. In this work, ultrathin amorphous carbon shells and lattice defects (heteroatoms and vacancies) are introduced into the MnNbO nanofiber surface to improve the electron/ion kinetic stability, conductivity and electrochemical activity. The ultrathin carbon interface protects unstable lattice with defects, thus restraining the adverse reaction between bimetallic oxides and electrolyte. Especially, ultrathin amorphous carbon layer enhances the stability and uniformity of ion transport as the substitute of solid-liquid ion exchange membrane. Lattice defects (N doping and oxygen vacancy) also enhance the ionic kinetics of the material. MnNbO nanofiber, being optimized by interface protection and lattice defects, shows excellent electrochemical performances in Lithium-ion battery and supercapacitor.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jcis.2021.08.001DOI Listing
August 2021

Engineering and modeling perspectives on photocatalytic reactors for water treatment.

Water Res 2021 Sep 11;202:117421. Epub 2021 Jul 11.

Environmental Nanocatalysis & Photoreaction Engineering, Department of Chemical Engineering, Loughborough University, Loughborough LE11 3TU, United Kingdom. Electronic address:

The debate on whether photocatalysis can reach full maturity at commercial level as an effective and economical process for treatment and purification of water and wastewater has recently intensified. Despite a bloom of scientific investigations in the last 30 years, particularly with regards to innovative photocatalytic materials, photocatalysis has so far seen a few industrial applications. Regardless of the points of view, it has been realized that research on reactor design and modeling are now equally urgent to match the extensive research carried out on innovative photocatalytic materials. In reality, the development of photocatalytic reactors has advanced steadily in terms of modeling and reactor design over the last two decades, though this topic has captured a smaller specialized audience. In this critical review, we introduce the latest developments on photocatalytic reactors for water treatment from an engineering perspective. The focus is on the modeling and design of photocatalytic reactors for water treatment at pilot- or at greater scale. Photocatalytic reactors utilizing both natural sunlight and UV irradiation sources are comprehensively discussed. The most promising photoreactor designs and models are examined giving key design guidelines. Other engineering considerations, such as operation, cost analysis, patents, and several industrial applications of photocatalytic reactors for water treatment are also presented. The dissemination of key photocatalytic reactor design principles among the scientific community and the water industry is currently one of the greatest obstacles in translating PWT research into widespread real-world application.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.watres.2021.117421DOI Listing
September 2021

High stability ultra-narrow band self-activated KGaSiO long-persistent phosphors for optical anti-counterfeiting.

Opt Lett 2021 Aug;46(16):3829-3832

Optical anti-counterfeiting has been developed as a promising optical-sensing technique. A self-activated phosphor was successfully prepared using the traditional solid-state method. The photoluminescence spectra of the as-synthesized phosphors indicate that the ultra-narrow band emission with green light peak at 503 nm is obtained when phosphors are excited by 254 nm UV light. Additionally, the measured afterglow curve shows that the emission of this phosphor can last more than 1200 s after UV excitation stops, which indicates that is a potential candidate for anti-counterfeiting materials. The luminescent and decay mechanism are discussed by theoretical calculation and thermo-luminescent spectra in detail. The theoretical model can provide support for explaining the mechanism of narrow band or persistent phosphor.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1364/OL.429877DOI Listing
August 2021

The complete chloroplast genome of a X.W.Li et G.S.

Mitochondrial DNA B Resour 2021 28;6(9):2514-2516. Epub 2021 Jul 28.

Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China Ministry of Education, Southwest Forestry University, Kunming, China.

X.W.Li et G.S is a plant species belonged to the family Papilionaceae. is currently found in broad-leaved forests in the limestone area of Luxi County, Yunnan Province. It is suitable for afforestation and urban greening in limestone areas. In this study, for the first time, we report the complete chloroplast genome sequence of . We sequenced and assembled the entire chloroplast genome of The chloroplast genome was determined to be 158,250 bp in length. It contained large single-copy (LSC) and small single-copy (SSC) regions of 84,930 bp and 12,664 bp, respectively, which were separated by a pair of inverted repeats (IR) regions of 30,328 bp. The genome contained 132 genes, including 87 protein-coding genes, 8 rRNA genes, and 37 tRNA genes. The overall GC content of the whole genome is 38.1%, and the corresponding values of the LSC, SSC, and IR regions were 36.4%, 33.6%, and 41.3%, respectively. Phylogenetic analysis suggested that is closely related to the genus in the Papilionaceae.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/23802359.2021.1959430DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8330731PMC
July 2021

Current Landscape: The Mechanism and Therapeutic Impact of Obesity for Breast Cancer.

Front Oncol 2021 19;11:704893. Epub 2021 Jul 19.

Department of Plastic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Obesity is defined as a chronic disease induced by an imbalance of energy homeostasis. Obesity is a widespread health problem with increasing prevalence worldwide. Breast cancer (BC) has already been the most common cancer and one of the leading causes of cancer death in women worldwide. Nowadays, the impact of the rising prevalence of obesity has been recognized as a nonnegligible issue for BC development, outcome, and management. Adipokines, insulin and insulin-like growth factor, sex hormone and the chronic inflammation state play critical roles in the vicious crosstalk between obesity and BC. Furthermore, obesity can affect the efficacy and side effects of multiple therapies such as surgery, radiotherapy, chemotherapy, endocrine therapy, immunotherapy and weight management of BC. In this review, we focus on the current landscape of the mechanisms of obesity in fueling BC and the impact of obesity on diverse therapeutic interventions. An in-depth exploration of the underlying mechanisms linking obesity and BC will improve the efficiency of the existing treatments and even provide novel treatment strategies for BC treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fonc.2021.704893DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8326839PMC
July 2021

Molecular typing of human adenoviruses among hospitalized patients with respiratory tract infections in a tertiary Hospital in Guangzhou, China between 2017 and 2019.

BMC Infect Dis 2021 Aug 3;21(1):748. Epub 2021 Aug 3.

Emergency Department, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, No. 111 Dade Road, Yuexiu District, Guangzhou, China.

Background: Human Adenoviruses (HAdVs) cause a wide array of illnesses in all age groups. They particularly cause frequent morbidity among children. In China, human adenovirus types 3, 4, 7, 11, 14, 21, and 55 have caused at least seven outbreaks since 2000. However, limited studies are available regarding the epidemiological patterns and diversity of HAdVs types among hospitalized patients with respiratory tract infections (RTIs).

Methods: To understand the epidemiology and subtype distribution of HAdV infections associated with RTIs in China, nasal swab (NS) clinical samples were collected from 4129 patients in a Guangzhou hospital between August 2017 and October 2019. PCR, sequencing, and phylogenetic analysis were performed on these specimens to identify HAdV subtypes.

Results: HAdV was successfully sequenced in 99 (2.4%) of the 4129 NS specimens, with the highest HAdV prevalence (6.3%) found in children between the ages of 5 and 10 years. Among HAdV-positive specimens, the most prevalent genotypes identified were HAdV-B3 (55.6%) and HAdV-B7 (25.3%). The most common symptoms in the HAdV-infected patients were fever (100%), cough (80.8%), and rhinorrhea (71.8%). HAdV infections were detected throughout the year with a relatively higher prevalence in summer.

Conclusion: All ages suffer adenovirus infections, but young children are at the greatest risk. This study data demonstrates that at least three species of HAdVs (species B, C, and E) are circulating in Guangzhou City, China. As antiviral therapies and type-specific vaccines become available, such epidemiological data will be useful in guiding therapy and public health interventions.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12879-021-06412-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8330471PMC
August 2021

Comprehensive Profiling of Inflammatory Factors Revealed That Growth Differentiation Factor-15 Is an Indicator of Disease Severity in COVID-19 Patients.

Front Immunol 2021 15;12:662465. Epub 2021 Jul 15.

Department of Laboratory Medicine, Shunde Hospital of Guangzhou University of Chinese Medicine, Foshan, China.

To systematically explore potential biomarkers which can predict disease severity in COVID-19 patients and prevent the occurrence or development of severe COVID-19, the levels of 440 factors were analyzed in patients categorized according to COVID-19 disease severity; including asymptomatic, mild, moderate, severe, convalescent and healthy control groups. Factor candidates were validated by ELISA and functional relevance was uncovered by bioinformatics analysis. To identify potential biomarkers of occurrence or development of COVID-19, patient sera from three different severity groups (moderate, severe, and critical) at three time points (admission, remission, and discharge) and the expression levels of candidate biomarkers were measured. Eleven differential factors associated with disease severity were pinpointed from 440 factors across 111 patients of differing disease severity. The dynamic changes of GDF15 reflect the progression of the disease, while the other differential factors include TRAIL R1, IGFBP-1, IGFBP-4, VCAM-1, sFRP-3, FABP2, Transferrin, GDF15, IL-1F7, IL-5Rα, and CD200. Elevation of white blood cell count, neutrophil count, neutrophil-lymphocyte ratio (NLR), Alanine aminotransferase and Aspartate aminotransferase, low lymphocyte and eosinophil counts in the severe group were associated with the severity of COVID-19. GDF15 levels were observed to be associated with the severity of COVID-19 and the dynamic change of GDF15 levels was closely associated with the COVID-19 disease progression. Therefore, GDF15 might serve as an indicator of disease severity in COVID-19 patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fimmu.2021.662465DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8320433PMC
August 2021

Amadis: A Comprehensive Database for Association Between Microbiota and Disease.

Front Physiol 2021 14;12:697059. Epub 2021 Jul 14.

Department of General Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, China.

The human gastrointestinal tract represents a symbiotic bioreactor that can mediate the interaction of the human host. The deployment and integration of multi-omics technologies have depicted a more complete image of the functions performed by microbial organisms. In addition, a large amount of data has been generated in a short time. However, researchers struggling to keep track of these mountains of information need a way to conveniently gain a comprehensive understanding of the relationship between microbiota and human diseases. To tackle this issue, we developed Amadis (http://gift2disease.net/GIFTED), a manually curated database that provides experimentally supported microbiota-disease associations and a dynamic network construction method. The current version of the Amadis database documents 20167 associations between 221 human diseases and 774 gut microbes across 17 species, curated from more than 1000 articles. By using the curated data, users can freely select and combine modules to obtain a specific microbe-based human disease network. Additionally, Amadis provides a user-friendly interface for browsing, searching and downloading. We hope it can serve as a useful and valuable resource for researchers exploring the associations between gastrointestinal microbiota and human diseases.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fphys.2021.697059DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8317061PMC
July 2021

Enhanced Electrochemical Reduction of CO to CO on Ag/SnO by a Synergistic Effect of Morphology and Structural Defects.

Chem Asian J 2021 Sep 12;16(18):2694-2701. Epub 2021 Aug 12.

Beijing Advanced Innovation Center for Materials Genome Engineering, Beijing Key Laboratory for Magneto-Photoelectrical Composite and Interface Science, School of Mathematics and Physics, University of Science and Technology Beijing, 100083, Beijing, P. R. China.

Silver (Ag)-based materials are considered to be promising materials for electrochemical reduction of CO to produce CO, but the selectivity and efficiency of traditional polycrystalline Ag materials are insufficient; there still exists a great challenge to explore novel modified Ag based materials. Herein, a nanocomposite of Ag and SnO (Ag/SnO ) for efficient reduction of CO to CO is reported. HRTEM and XRD patterns clearly demonstrated the lattice destruction of Ag and the amorphous SnO in the Ag/SnO nanocomposite. Electrochemical tests indicated the nanocomposite containing 15% SnO possesses highest catalytic selectivity featured by a CO faradaic efficiency (FE) of 99.2% at -0.9 V versus reversible hydrogen electrode (vs RHE) and FE>90% for the CO product at a wide potential range from -0.8 V to -1.4 V vs RHE. Experimental characterization and analysis showed that the high catalytic performance is attributed to not only the branched morphology of Ag/SnO nanocomposites (NCs), which endows the maximum exposure of active sites, but also the special adsorption capacity of abundant defect sites in the crystal for *COOH (the key intermediate of CO formation), which improves the intrinsic activity of the catalyst. But equally important, the existed SnO also plays an important role in inhibiting hydrogen evolution reaction (HER) and anchoring defect sites. This work demonstrates the use of crystal defect engineering and synergy in composite to improve the efficiency of electrocatalytic CO reduction reaction (CO RR).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/asia.202100718DOI Listing
September 2021

F-ASEM Imaging for Evaluating Atherosclerotic Plaques Linked to α7-Nicotinic Acetylcholine Receptor.

Front Bioeng Biotechnol 2021 1;9:684221. Epub 2021 Jul 1.

Department of Cardiovascular Surgery, Fu Wai Hospital, Cardiovascular Institute, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Background: Atherosclerosis is a chronic vascular inflammatory procedure alongside with lipid efflux disorder and foam cell formation. α7-Nicotinic acetylcholine receptor (α7nAChR) is a gated-calcium transmembrane channel widely expressed in neuron and non-neuron cells, such as monocytes and macrophages, activated T cells, dendritic cells, and mast cells. F-ASEM is an inhibitor targeted to α7nAChR that had been successfully applied in nervous system diseases. Previous studies had highlighted that α7nAChR was related to the emergency of vulnerable atherosclerotic plaques with excess inflammation cells. Thus, F-ASEM could be a complementary diagnostic approach to atherosclerotic plaques.

Materials And Methods: The synthesis of ASEM precursor and F-labeling had been performed successfully. We had established the ApoE mice atherosclerotic plaques model (fed with western diet) and New Zealand rabbits atherosclerotic models (balloon-sprained experiment and western diet). After damage of endothelial cells and primary plaque formation, F-ASEM imaging of atherosclerotic plaques linked to α7nAChR had been conducted. micro-PET/CT imaging of ApoE mice and the control group was performed 1 h after injection of F-ASEM (100-150 μCi); PET/CT imaging for rabbits with atherosclerotic plaques and control ones was also performed. Meanwhile, we also conducted CT scan on the abdominal aorta of these rabbits. After that, the animals were sacrificed, and the carotid and abdominal aorta were separately taken out for circular sections. The paraffin-embedded specimens were sectioned with 5 μm thickness and stained with hematoxylin-eosin (H&E) and oil red.

Results: vessel binding of F-ASEM and α7nAChR expression in the model group with atherosclerosis plaques was significantly higher than that in the control group. PET/CT imaging successfully identified the atherosclerotic plaques in ApoE mice and model rabbits, whereas no obvious signals were detected in normal mice or rabbits. Compared with F-FDG, F-ASEM had more significant effect on the early monitoring of inflammation in carotid atherosclerotic plaques of ApoE mice and model rabbits. F-ASEM had relatively more palpable effect on the imaging of abdominal aorta with atherosclerosis in rabbits. H&E and oil red staining identified the formation of atherosclerotic plaques in model animals, which provided pathological basis for the evaluation of imaging effects.

Conclusion: We first confirmed F-ASEM as radiotracer with good imaging properties for precise identification of atherosclerotic diseases.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fbioe.2021.684221DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8280778PMC
July 2021

Bioaugmentation mitigates ammonia and hydrogen sulfide emissions during the mixture compost of dewatered sewage sludge and reed straw.

Environ Sci Pollut Res Int 2021 Jul 17. Epub 2021 Jul 17.

School of Environmental and Municipal Engineering, North China University of Water Resources and Electric Power, No. 36, Beihuan Road, Zhengzhou, 450045, Henan, China.

This study investigated the effectiveness of bio-augmenting aerobic cell culture to mitigate ammonia and hydrogen sulfide emission in sewage sludge composting amended with reed straw (with the weight ratio of 1:0.3-0.4). During the 20-day aerated lab-scale composting, adding 200-mL culture (56.80 NTU) reduced ammonia and hydrogen sulfide emissions by 38.00% and 54.32%, and conserved total nitrogen and sulfate by 39.42% and 70.75%, respectively. Organic matters degradation was quick started 1 day ahead. Comparing to the control, nitrate content increased 38.75% at the end of the compost. Bioaugmentation evened the distributions of bacterial communities in the thermophilic phase. The shift was mainly due to 22.97% of relative abundance of Proteobacteria depressed and 157.16% of Bacteroidetes increased, which were beneficial for nitrogen conservation and glycan breakdown, respectively. In summary, the results demonstrated that bioaugmentation addition could be an effective strategy for enhanced sludge composting.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11356-021-15446-5DOI Listing
July 2021

Chromatic dispersion equalization FIR filter design based on discrete least-squares approximation.

Opt Express 2021 Jun;29(13):20387-20394

Chromatic dispersion (CD) equalization is one of the core tasks of the digital signal processing (DSP) chain in modern optical coherent receivers. A conventional impulse-invariant method for designing the CD equalization filter is revisited, improved by proper weighting, and reinterpreted as a Fourier series. To improve upon a direct evaluation of the passband least-squares (LS) approximation, we propose to design a CD equalization finite impulse response (FIR) filter based on a discrete LS approximation. The proposed method avoids numerical evaluation of nontrivial functions and relies only on Fourier transform. Its flexibility is corroborated by a filter design demonstration of joint matched filtering and CD equalization.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1364/OE.430955DOI Listing
June 2021

Modified square timing error detector with large chromatic dispersion tolerance for optical coherent receivers.

Opt Express 2021 Jun;29(13):19759-19766

Clock recovery plays an important role in the digital signal processing (DSP) chain of modern coherent optical receivers. It references the local sampling clock with the signal baudrate and finds the optimal sampling instances by performing endless timing error corrections. At the core of clock recovery, a timing error detector (TED) is used to provide instantaneous error tracking. However, usual TEDs suffer from effects such as chromatic dispersion (CD) and polarization rotation, thus requiring additional efforts to remove those effects before TED. Here we propose a modified square TED based on the signal's cyclic autocorrelation function (CAF), which generalizes its classical counterpart and exhibits a much larger CD tolerance. It provides a time-domain solution of the CD-tolerant TED. The previously analyzed equivalence among the time-domain and the frequency-domain TEDs is reestablished in the framework of spectral correlation. The modified square TED demands a minimum extra complexity. Both numerical simulation and experiments are performed to study the performance of the proposed TED.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1364/OE.428015DOI Listing
June 2021

A single luminescence center ultra-broadband near-infrared LiScGeO:Cr phosphor for biological tissue penetration.

Dalton Trans 2021 Jul;50(29):10092-10101

National-Local Joint Engineering Laboratory of New Energy Photoelectric Devices, Hebei Key Laboratory of Optic-electronic Information and Materials, College of Physics Science & Technology, Hebei University, Baoding 071002, China.

In this work, in order to meet the application of near-infrared phosphor-converted light emitting diodes (pc-LEDs), an ultra-broadband emission phosphor, LiScGeO4:Cr, was synthesized. Its FWHM reaches 335 nm, and its emission spectrum ranges from 800 nm to 1650 nm, which almost covers the entire near-infrared second window (NIR-II). The broadband emission is thought to be caused by the 4T2 → 4A2 transition of the Cr3+ ion. This transition occurs due to the olivine structure of the crystal, which causes the Cr3+ ions to inhabit a low-symmetric crystal field, and the crystal field strength is very weak. NIR pc-LEDs were fabricated by combining a 460 nm blue LED with this phosphor, which penetrates 4 cm thick beef. The results indicate that there may be a potential application for this phosphor in the field of biological tissue penetration and non-destructive testing.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d1dt01244dDOI Listing
July 2021

The complete chloroplast genome sequence of , a species endemic to China.

Mitochondrial DNA B Resour 2021 Jun 15;6(7):1906-1908. Epub 2021 Jun 15.

Wetland Management Department of Yunnan Forestry and Grassland Bureau, Kunming, PR China.

, widely distributed in central to southwest of China, is an economically important forest tree. The chloroplast genome is 159,861 bp in length with a typical circular quadripartite structure, containing 129 genes (84 protein-coding genes, 37 tRNAs, and eight rRNAs). Our phylogenetic result clearly showed that and have the closest relationship.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/23802359.2021.1935338DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8208118PMC
June 2021

CRISPR-Cas13a-Based Detection for Bovine Viral Diarrhea Virus.

Front Vet Sci 2021 9;8:603919. Epub 2021 Jun 9.

College of Life Sciences, Shihezi University, Shihezi, China.

Bovine Viral Diarrhea Virus (BVDV) is the main pathogen of bovine viral diarrhea disease (BVD), which leads to enormous economic losses in the cattle industry. A sensitive and specific detection for BVDV is advantageous to the control of BVDV. Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas systems have been used for detecting virus RNA. In this study, the expression and purification of LwCas13a protein was optimized and the RNase activity of LwCas13a was verified. CRISPR-LwCas13a system could detect BVDV virus and BVDV RNA with high specificity and simplicity. The detection limit of the LwCas13a system was 10 pM, and there were no cross-reactions with HEK293T and MDBK. In summary, a sensitive, specific, and simple nucleic acid detection method based on CRISPR-Cas13a was developed for BVDV. This method provides a new detection strategy for early diagnosis of BVDV.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fvets.2021.603919DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8219879PMC
June 2021

Ramp lesion of the medial meniscus.

EFORT Open Rev 2021 May 4;6(5):372-379. Epub 2021 May 4.

Department of Orthopaedics, the Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai, Guangdong, China.

Ramp lesion of the medial meniscus used to be completely disregarded in the past.Ramp lesion has been now put under the spotlight by orthopaedic and sport medicine surgeons and requires attention.It is closely associated with anterior cruciate ligament injury. Major risk factors include chronic laxity, lateral meniscal lesion, anterior cruciate ligament reconstruction revision, anterolateral ligament tear concomitant with anterior cruciate ligament injury, time from injury, pre-operative side-to-side laxity > 6 mm, age < 30 years old, male sex, etc.Radiologists attempt to create diagnostic criteria for ramp lesion using magnetic resonance imaging. However, the only definite method to diagnose ramp lesion is still arthroscopy. Various techniques exist, among which posteromedial approach is the most highly recommended.Various treatment options are available. The success rate of ramp repair is very high. Major complications are uncommon. Cite this article: 2021;6:372-379. DOI: 10.1302/2058-5241.6.200126.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1302/2058-5241.6.200126DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8183152PMC
May 2021

ZhiJingSan Inhibits Osteoclastogenesis via Regulating RANKL/NF-κB Signaling Pathway and Ameliorates Bone Erosion in Collagen-Induced Mouse Arthritis.

Front Pharmacol 2021 28;12:693777. Epub 2021 May 28.

Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, China.

Bone erosion is the most evident pathological condition of rheumatoid arthritis (RA), which is the main cause of joint deformities and disability in RA patients. At present, the conventional RA drugs have not achieved satisfactory effect in improving bone erosion. ZhiJingSan (ZJS), which is a traditional Chinese prescription composed of scolopendra (dried body of , ) and scorpion (dried body of ), exhibits anti-rheumatism, analgesic and joint deformities improvement effects. This study aimed to assess the therapeutic effect of ZJS on RA bone erosion and to elucidate the underlying mechanism. The effect of ZJS on RA bone erosion was investigated in a murine model of bovine collagen-induced arthritis (CIA), and the underlying mechanism was investigated in an osteoclast differentiation cell model. Administration of ZJS delayed the onset of arthritis, alleviated joint inflammation, and attenuated bone erosion in the CIA mice. Meanwhile, ZJS decreased the serum levels of TNF-α, IL-6, and anti-bovine collagen II-specific antibodies. Furthermore, ZJS treatment reduced the number of osteoclasts and the expression of cathepsin K in the ankle joints of CIA mice. ZJS also inhibited receptor activator of NF-κB ligand (RANKL)-induced osteoclast differentiation and the expression of MMP9 and cathepsin K . Mechanistically, ZJS blocked RANKL-induced p65 phosphorylation, nucleation, and inhibited the expression of downstream NFATc1 and c-Fos in bone marrow-derived macrophages (BMMs). Taken together, ZJS exerts a therapeutic effect on bone erosion in CIA mice by inhibiting RANKL/NF-κB-mediated osteoclast differentiation, which suggested that ZJS is a promising prescription for treating RA bone erosion.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fphar.2021.693777DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193094PMC
May 2021

PGC-1 Protects against Hepatic Ischemia Reperfusion Injury by Activating PPAR and PPAR and Regulating ROS Production.

Oxid Med Cell Longev 2021 19;2021:6677955. Epub 2021 May 19.

Key Laboratory of Hepatosplenic Surgery, Ministry of Education, Department of General Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, China.

Peroxisome proliferator-activated receptors (PPARs) and have been shown to be protective in hepatic ischemia/reperfusion (I/R) injury. However, the precise role of PPAR coactivator-1 (PGC-1), which can coactivate both of these receptors, in hepatic I/R injury, remains largely unknown. This study was designed to test our hypothesis that PGC-1 is protective during hepatic I/R injury in vitro and in vivo. Our results show that endogenous PGC-1 is basally expressed in normal livers and is moderately increased by I/R. Ectopic PGC-1 protects against hepatic I/R and hepatocyte anoxia/reoxygenation (A/R) injuries, whereas knockdown of endogenous PGC-1 aggravates such injuries, as evidenced by assessment of the levels of serum aminotransferases and inflammatory cytokines, necrosis, apoptosis, cell viability, and histological examination. The EMSA assay shows that the activation of PPAR and PPAR is increased or decreased by the overexpression or knockdown of PGC-1, respectively, during hepatic I/R and hepatocyte A/R injuries. In addition, the administration of specific antagonists of either PPAR (MK886) or PPAR (GW9662) can effectively decrease the protective effect of PGC-1 against hepatic I/R and hepatocyte A/R injuries. We also demonstrate an important regulatory role of PGC-1 in reactive oxygen species (ROS) metabolism during hepatic I/R, which is correlated with the induction of ROS-detoxifying enzymes and is also dependent on the activations of PPAR and PPAR. These data demonstrate that PGC-1 protects against hepatic I/R injury, mainly by regulating the activation of PPAR and PPAR. Thus, PGC-1 may be a promising therapeutic target for the protection of the liver against I/R injury.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2021/6677955DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8159639PMC
May 2021

A reconstructed porous copper surface promotes selectivity and efficiency toward C products by electrocatalytic CO reduction.

Chem Sci 2020 May 19;11(39):10698-10704. Epub 2020 May 19.

CAS Key Laboratory of Nanosystem and Hierarchical Fabrication, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology Beijing 100190 P. R. China

Electrocatalytic synthesis of multicarbon (C) products from CO reduction suffers from poor selectivity and low energy efficiency. Herein, a facile oxidation-reduction cycling method is adopted to reconstruct the Cu electrode surface with the help of halide anions. The surface composed of entangled Cu nanowires with hierarchical pores is synthesized in the presence of I, exhibiting a C faradaic efficiency (FE) of 80% at -1.09 V RHE. A partial current density of 21 mA cm is achieved with a C half-cell power conversion efficiency (PCE) of 39% on this electrode. Such high selective C production is found to mainly originate from CO intermediate enrichment inside hierarchical pores rather than the surface lattice effect of the Cu electrode.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d0sc01202eDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8162280PMC
May 2020

Tumor-derived exosomal components: the multifaceted roles and mechanisms in breast cancer metastasis.

Cell Death Dis 2021 05 26;12(6):547. Epub 2021 May 26.

Department of Plastic and Cosmetic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430030, Wuhan, China.

Breast cancer (BC) is the most frequently invasive malignancy and the leading cause of tumor-related mortality among women worldwide. Cancer metastasis is a complex, multistage process, which eventually causes tumor cells to colonize and grow at the metastatic site. Distant organ metastases are the major obstacles to the management of advanced BC patients. Notably, exosomes are defined as specialized membrane-enclosed extracellular vesicles with specific biomarkers, which are found in a wide variety of body fluids. Recent studies have demonstrated that exosomes are essential mediators in shaping the tumor microenvironment and BC metastasis. The transferred tumor-derived exosomes modify the capability of invasive behavior and organ-specific metastasis in recipient cells. BC exosomal components, mainly including noncoding RNAs (ncRNAs), proteins, lipids, are the most investigated components in BC metastasis. In this review, we have emphasized the multifaceted roles and mechanisms of tumor-derived exosomes in BC metastasis based on these important components. The underlying mechanisms mainly include the invasion behavior change, tumor vascularization, the disruption of the vascular barrier, and the colonization of the targeted organ. Understanding the significance of tumor-derived exosomal components in BC metastasis is critical for yielding novel routes of BC intervention.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41419-021-03825-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8155106PMC
May 2021

Effects of biological nitrification inhibitors on nitrogen use efficiency and greenhouse gas emissions in agricultural soils: A review.

Ecotoxicol Environ Saf 2021 Sep 18;220:112338. Epub 2021 May 18.

State Key Laboratory of Water Environment Simulation, School of Environment, Beijing Normal University, Beijing 100875, China.

To maintain and increase crop yields, large amounts of nitrogen fertilizers have been applied to farmland. However, the nitrogen use efficiency (NUE) of chemical fertilizer remains very low, which may lead to serious environmental problems, including nitrate pollution, air quality degradation and greenhouse gas (GHG) emissions. Nitrification inhibitors can alleviate nitrogen loss by inhibiting nitrification; thus, biological nitrification inhibition by plants has gradually attracted increasing attention due to its low cost and environmental friendliness. Research progress on BNI is reviewed in this article, including the source, mechanisms, influencing factors and application of BNIs. In addition, the impact of BNI on agriculture and GHG emissions is summarized from the perspective of agricultural production and environmental protection, and the key future research prospects of BNIs are also noted.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ecoenv.2021.112338DOI Listing
September 2021
-->