David Hacker

David Hacker

UNVERIFIED PROFILE

Are you David Hacker?   Register this Author

Register author
David Hacker

David Hacker

Publications by authors named "David Hacker"

Are you David Hacker?   Register this Author

94Publications

1309Reads

30Profile Views

CHO and HEK293 Cultivation and Transfection in Single-Use Orbitally Shaken Bioreactors.

Methods Mol Biol 2018 ;1850:123-131

Protein Production and Structure Core Facility (PPSCF), Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/978-1-4939-8730-6_9DOI Listing
May 2019

BRB-seq: ultra-affordable high-throughput transcriptomics enabled by bulk RNA barcoding and sequencing.

Genome Biol 2019 04 19;20(1):71. Epub 2019 Apr 19.

Institute of Bioengineering, School of Life Sciences, École Polytechnique Fédérale de Lausanne (EPFL), CH-1015, Lausanne, Switzerland.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13059-019-1671-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6474054PMC
April 2019

Cognitive Behavioral Therapy Plus Standard Care Versus Standard Care Plus Other Psychosocial Treatments for People With Schizophrenia.

Schizophr Bull 2019 03;45(2):284-286

Department of Clinical Psychology, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1093/schbul/sby188DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6403053PMC
March 2019

Cognitive behavioural therapy plus standard care versus standard care for people with schizophrenia.

Cochrane Database Syst Rev 2018 12 20;12:CD007964. Epub 2018 Dec 20.

School of Psychology, University of Birmingham, Edgbaston, Birmingham, UK, B15 2TT.

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1002/14651858.CD007964.pub2
Publisher Site
http://dx.doi.org/10.1002/14651858.CD007964.pub2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6517137PMC
December 2018

Becoming American: Intermarriage during the Great Migration to the United States.

J Interdiscip Hist 2018 31;49(2):189-218. Epub 2018 Aug 31.

University of Bologna.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1162/jinh_a_01266DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6746435PMC
August 2018

Large-Scale Transient Transfection of Chinese Hamster Ovary Cells in Suspension.

Methods Mol Biol 2017 ;1603:45-55

Laboratory of Cellular Biotechnology (LBTC), École Polytechnique Fédérale de Lausanne (EPFL), CH-1015, Lausanne, Switzerland.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/978-1-4939-6972-2_3DOI Listing
February 2018

Improved process conditions for increasing expression of MHC class II protein from a stable Drosophila S2 cell line.

Biotechnol Lett 2018 Jan 9;40(1):85-92. Epub 2017 Oct 9.

Laboratory of Cellular Biotechnology, Faculty of Life Sciences, École Polytechnique Fédérale de Lausanne, 1015, Lausanne, Switzerland.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10529-017-2440-0DOI Listing
January 2018

Expression proteomics study to determine metallodrug targets and optimal drug combinations.

Sci Rep 2017 05 8;7(1):1590. Epub 2017 May 8.

Institute of Chemical Sciences and Engineering, Swiss Federal Institute of Technology Lausanne (EPFL), CH-1015, Lausanne, Switzerland.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-017-01643-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5431558PMC
May 2017

Genetic incorporation of 4-fluorohistidine into peptides enables selective affinity purification.

Org Biomol Chem 2017 May;15(21):4536-4539

Virginia Commonwealth University, Department of Chemistry, 1001 West Main Street, Richmond, Virginia 23284-2006, USA.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/c7ob00844aDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6010304PMC
May 2017

Highly Constrained Bicyclic Scaffolds for the Discovery of Protease-Stable Peptides via mRNA Display.

ACS Chem Biol 2017 03 1;12(3):795-804. Epub 2017 Feb 1.

Virginia Commonwealth University , Department of Chemistry, 1001 West Main Street, Richmond, Virginia 23284-2006, United States.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1021/acschembio.6b01006DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5443354PMC
March 2017

Studies on fluid dynamics of the flow field and gas transfer in orbitally shaken tubes.

Biotechnol Prog 2017 01 31;33(1):192-200. Epub 2016 Oct 31.

Laboratory of Cellular Biotechnology (LBTC), Faculty of Life Sciences, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, CH-1015, Switzerland.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/btpr.2375DOI Listing
January 2017

Ready, Willing, and Able? Impediments to the Onset of Marital Fertility Decline in the United States.

Authors:
J David Hacker

Demography 2016 12;53(6):1657-1692

Department of History, College of Liberal Arts, University of Minnesota, 1110 Heller Hall/Del. Code 7062, 271 19th Avenue South, Minneapolis, MN, 55455, USA.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s13524-016-0513-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5130602PMC
December 2016

Enhanced plasmid DNA utilization in transiently transfected CHO-DG44 cells in the presence of polar solvents.

Biotechnol Prog 2015 Nov-Dec;31(6):1571-8. Epub 2015 Aug 25.

Laboratory for Cellular Biotechnology (LBTC) and Protein Expression Core Facility (PECF), École Polytechnique Fédérale De Lausanne (EPFL), Lausanne, 1015, Switzerland.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/btpr.2152DOI Listing
October 2016

Multigene expression in stable CHO cell pools generated with the piggyBac transposon system.

Biotechnol Prog 2016 09 13;32(5):1308-1317. Epub 2016 Jul 13.

Laboratory of Cellular Biotechnology (LBTC), École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, CH-1015, Switzerland.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/btpr.2319DOI Listing
September 2016

A comparison of orbitally-shaken and stirred-tank bioreactors: pH modulation and bioreactor type affect CHO cell growth and protein glycosylation.

Biotechnol Prog 2016 09 11;32(5):1174-1180. Epub 2016 Aug 11.

Laboratory of Cellular Biotechnology (LBTC), École Polytechnique Fédérale De Lausanne (EPFL), Lausanne, CH-1015, Switzerland.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/btpr.2328DOI Listing
September 2016

Disposable orbitally shaken TubeSpin bioreactor 600 for Sf9 cell cultivation in suspension.

Anal Biochem 2016 07 27;505:26-8. Epub 2016 Apr 27.

Laboratory of Cellular Biotechnology (LBTC), École Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland. Electronic address:

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ab.2016.04.009DOI Listing
July 2016

Comparison of three transposons for the generation of highly productive recombinant CHO cell pools and cell lines.

Biotechnol Bioeng 2016 06 9;113(6):1234-43. Epub 2015 Dec 9.

Laboratory of Cellular Biotechnology, École Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/bit.25888DOI Listing
June 2016

Recombinant protein production from stable mammalian cell lines and pools.

Curr Opin Struct Biol 2016 06 17;38:129-36. Epub 2016 Jun 17.

Laboratory of Cellular Biotechnology (LBTC), École Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.sbi.2016.06.005DOI Listing
June 2016

Transcriptional and post-transcriptional limitations of high-yielding, PEI-mediated transient transfection with CHO and HEK-293E cells.

Biotechnol Prog 2015 Mar-Apr;31(2):541-9. Epub 2015 Feb 26.

Laboratory for Cellular Biotechnology (LBTC), École Polytechnique Fédérale de Lausanne (EPFL), CH-1015, Lausanne, Switzerland.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/btpr.2064DOI Listing
January 2016

Production of active glycosylation-deficient γ-secretase complex for crystallization studies.

Biotechnol Bioeng 2015 Dec 14;112(12):2516-26. Epub 2015 Jul 14.

Laboratory of Molecular and Cellular Biology of Alzheimer's Disease, Brain Mind Institute and School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH1015 Lausanne, Switzerland.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/bit.25675DOI Listing
December 2015

A simple plasmid-based transient gene expression method using High Five cells.

J Biotechnol 2015 Dec 23;216:67-75. Epub 2015 Oct 23.

Laboratory of Cellular Biotechnology, Faculty of Life Sciences, École Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland. Electronic address:

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jbiotec.2015.10.007DOI Listing
December 2015

Peptide and peptide library cyclization via bromomethylbenzene derivatives.

Methods Mol Biol 2015 ;1248:105-17

Department of Chemistry and Massey Cancer Center, Virginia Commonwealth University, 401 College Street, Richmond, VA, 23298-0037, USA.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/978-1-4939-2020-4_8DOI Listing
September 2015

Population Variation and Genetic Control of Modular Chromatin Architecture in Humans.

Cell 2015 Aug 20;162(5):1039-50. Epub 2015 Aug 20.

Swiss Institute of Bioinformatics, Lausanne 1015, Switzerland; Department of Genetic Medicine and Development, University of Geneva Medical School, Geneva 1211, Switzerland; Institute of Genetics and Genomics in Geneva, University of Geneva, Geneva 1211, Switzerland. Electronic address:

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.cell.2015.08.001DOI Listing
August 2015

Peptide library approach to uncover phosphomimetic inhibitors of the BRCA1 C-terminal domain.

ACS Chem Biol 2015 May 5;10(5):1198-208. Epub 2015 Feb 5.

†Department of Chemistry, Virginia Commonwealth University (VCU), 1001 West Main Street, P.O. Box 842006, Richmond, Virginia 23284, United States.

View Article

Download full-text PDF

Source
http://pubs.acs.org/doi/10.1021/cb500757u
Publisher Site
http://dx.doi.org/10.1021/cb500757uDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4433557PMC
May 2015

Influence of glutamine on transient and stable recombinant protein production in CHO and HEK-293 cells.

BMC Proc 2011 22;5 Suppl 8:P35. Epub 2011 Nov 22.

Laboratory for Cellular Biotechnology (LBTC), École Polytechnique Fédéral de Lausanne (EPFL), CH-1015 Lausanne, Switzerland.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/1753-6561-5-S8-P35DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3284955PMC
April 2015

kLa as a predictor for probe-independent mammalian cell bioprocesses in orbitally shaken bioreactors.

BMC Proc 2011 22;5 Suppl 8:P36. Epub 2011 Nov 22.

Laboratory of Cellular Biotechnology, Faculty of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, 1015 Lausanne, Switzerland.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/1753-6561-5-S8-P36DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3397689PMC
April 2015

Transient transfection of insect Sf-9 cells in TubeSpin(®) bioreactor 50 tubes.

BMC Proc 2011 22;5 Suppl 8:P37. Epub 2011 Nov 22.

Laboratory of Cellular Biotechnology, Faculty of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/1753-6561-5-S8-P37DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3284885PMC
April 2015

Transient gene expression with CHO cells in conditioned medium: a study using TubeSpin(®) bioreactors.

BMC Proc 2011 22;5 Suppl 8:P38. Epub 2011 Nov 22.

Laboratory of Cellular Biotechnology, Faculty of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/1753-6561-5-S8-P38DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3284980PMC
April 2015

Hydrodynamic stress in orbitally shaken bioreactors.

BMC Proc 2011 22;5 Suppl 8:P39. Epub 2011 Nov 22.

Laboratory of Cellular Biotechnology, Faculty of Life Sciences, Ecole Polytechnique Fédérale de Lausanne.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/1753-6561-5-S8-P39DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3284983PMC
April 2015

CHO cell lines generated by PiggyBac transposition.

BMC Proc 2011 22;5 Suppl 8:P31. Epub 2011 Nov 22.

Laboratory of Cellular Biotechnology, Faculty of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland ; ExcellGene SA, CH-1870 Monthey, Switzerland.

View Article

Download full-text PDF

Source
http://bmcproc.biomedcentral.com/articles/10.1186/1753-6561-
Publisher Site
http://dx.doi.org/10.1186/1753-6561-5-S8-P31DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3284968PMC
April 2015

Transposon mediated co-integration and co-expression of transgenes in CHO-DG44 cells.

BMC Proc 2011 22;5 Suppl 8:P32. Epub 2011 Nov 22.

Laboratory for Cellular Biotechnology (LBTC), Faculty of Life Sciences, École Polytechnique Fédérale de Lausanne CH-1015 Lausanne, Switzerland.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/1753-6561-5-S8-P32DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3284971PMC
April 2015

The use of filler DNA for improved transfection and reduced DNA needs in transient gene expression with CHO and HEK cells.

BMC Proc 2011 22;5 Suppl 8:P33. Epub 2011 Nov 22.

Laboratory for Cellular Biotechnology, Faculty of Life Sciences, École Polytechnique Fédéral de Lausanne, CH-1015 Lausanne, Switzerland.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/1753-6561-5-S8-P33DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3285026PMC
April 2015

Rapid recombinant protein production from pools of transposon-generated CHO cells.

BMC Proc 2011 22;5 Suppl 8:P34. Epub 2011 Nov 22.

Laboratory of Cellular Biotechnology, Faculty of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland ; ExcellGene SA, CH-1870 Monthey, Switzerland.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/1753-6561-5-S8-P34DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3285008PMC
April 2015

Rapid recombinant protein production from piggyBac transposon-mediated stable CHO cell pools.

J Biotechnol 2015 Apr 7;200:61-9. Epub 2015 Mar 7.

Laboratory for Cellular Biotechnology, CH-1015 Lausanne, Switzerland. Electronic address:

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jbiotec.2015.03.001DOI Listing
April 2015

Recombinant therapeutic protein production in cultivated mammalian cells: current status and future prospects.

Drug Discov Today Technol 2008 ;5(2-3):e37-42

Institute of Bioengineering, Laboratory of Cellular Biotechnology, École Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland. Electronic

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ddtec.2008.12.003DOI Listing
July 2014

Use of orbital shaken disposable bioreactors for mammalian cell cultures from the milliliter-scale to the 1,000-liter scale.

Adv Biochem Eng Biotechnol 2009 ;115:33-53

Laboratory of Cellular Biotechnology, Ecole Polytechnique Fédérale de Lausanne, CH-1015, Lausanne, Switzerland.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/10_2008_18DOI Listing
May 2014

Virus-free transient protein production in Sf9 cells.

J Biotechnol 2014 Feb 11;171:61-70. Epub 2013 Dec 11.

Laboratory of Cellular Biotechnology, Faculty of Life Sciences, École Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland. Electronic address:

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jbiotec.2013.11.018DOI Listing
February 2014

Polyethyleneimine-based transient gene expression processes for suspension-adapted HEK-293E and CHO-DG44 cells.

Protein Expr Purif 2013 Nov 8;92(1):67-76. Epub 2013 Sep 8.

Protein Expression Core Facility, EPFL, CH-1015 Lausanne, Switzerland; Laboratory of Cellular Biotechnology, EPFL, CH-1015 Lausanne, Switzerland. Electronic address:

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.pep.2013.09.001DOI Listing
November 2013

DNA delivery with hyperbranched polylysine: a comparative study with linear and dendritic polylysine.

J Control Release 2013 Aug 1;169(3):276-88. Epub 2013 Feb 1.

Ecole Polytechnique Fédérale de Lausanne (EPFL), Institut des Matériaux et Institut des Sciences et Ingénierie Chimiques, Laboratoire des Polymères, Bâtiment MXD, Station 12, CH-1015 Lausanne, Switzerland.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jconrel.2013.01.019DOI Listing
August 2013

Highly efficient production of the Alzheimer's γ-secretase integral membrane protease complex by a multi-gene stable integration approach.

Biotechnol Bioeng 2013 Jul 15;110(7):1995-2005. Epub 2013 Feb 15.

Laboratory of Molecular & Cellular Biology of Alzheimer's Disease, Brain Mind Institute and School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH1015 Lausanne, Switzerland.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/bit.24851DOI Listing
July 2013

A NanoDrop-based method for rapid determination of viability decline in suspension cultures of animal cells.

Anal Biochem 2012 Nov 6;430(2):138-40. Epub 2012 Sep 6.

Laboratory of Cellular Biotechnology, École Polytechnique Fédérale de Lausanne (EPFL), School of Life Sciences, CH-1015 Lausanne, Switzerland.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ab.2012.08.028DOI Listing
November 2012

A census-based count of the Civil War dead.

Authors:
J David Hacker

Civ War Hist 2011 ;57(4):307-48

Binghamton University, SUNY.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1353/cwh.2011.0061DOI Listing
October 2012

Comparative study on the in vitro cytotoxicity of linear, dendritic, and hyperbranched polylysine analogues.

Biomacromolecules 2012 Oct 11;13(10):3127-37. Epub 2012 Sep 11.

Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1021/bm300930jDOI Listing
October 2012

Role of non-specific DNA in reducing coding DNA requirement for transient gene expression with CHO and HEK-293E cells.

Biotechnol Bioeng 2012 Sep 30;109(9):2271-8. Epub 2012 Mar 30.

Laboratory for Cellular Biotechnology, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/bit.24494DOI Listing
September 2012

Chemical macrocyclization of peptides fused to antibody Fc fragments.

Bioconjug Chem 2012 Sep 10;23(9):1856-63. Epub 2012 Aug 10.

Institute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1021/bc300184mDOI Listing
September 2012

Cognitive behavior therapy versus other psychosocial treatments for schizophrenia.

Schizophr Bull 2012 Sep;38(5):908-10

School of Psychology, University of Birmingham, Birmingham, UK.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1093/schbul/sbs090DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3446233PMC
September 2012

Hyperbranched polylysine: a versatile, biodegradable transfection agent for the production of recombinant proteins by transient gene expression and the transfection of primary cells.

Macromol Biosci 2012 Jun 11;12(6):794-804. Epub 2012 Apr 11.

Institut des Matériaux et Institut des Sciences et Ingénierie Chimiques, École Polytechnique Fédérale de Lausanne-EPFL, Bâtiment MXD, Station 12, CH-1015 Lausanne, Switzerland.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/mabi.201100519DOI Listing
June 2012

Poly(ethyleneimine)-mediated large-scale transient gene expression: influence of molecular weight, polydispersity and N-propionyl groups.

Macromol Biosci 2012 May 13;12(5):628-36. Epub 2012 Mar 13.

Laboratoire des Polymères, Institut des Matériaux et Institut des Sciences et Ingénieries Chimiques, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/mabi.201100404DOI Listing
May 2012

Reduced glutamine concentration improves protein production in growth-arrested CHO-DG44 and HEK-293E cells.

Biotechnol Lett 2012 Apr 30;34(4):619-26. Epub 2011 Nov 30.

Laboratory for Cellular Biotechnology (LBTC), École Polytechnique Fédéral de Lausanne (EPFL), 1015, Lausanne, Switzerland.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10529-011-0809-zDOI Listing
April 2012

Cognitive behaviour therapy versus other psychosocial treatments for schizophrenia.

Cochrane Database Syst Rev 2012 Apr 18(4):CD008712. Epub 2012 Apr 18.

School of Psychology, University of Birmingham, Birmingham, UK.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/14651858.CD008712.pub2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4163968PMC
April 2012

Developing a measurement of engagement: the Residential Rehabilitation Engagement Scale for psychosis.

J Ment Health 2012 Apr;21(2):182-91

Birmingham and Solihull Mental Health NHS Foundation Trust, Birmingham, UK.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3109/09638237.2012.664301DOI Listing
April 2012

Large-scale transfection of mammalian cells.

Methods Mol Biol 2012 ;801:13-26

Laboratory of Cellular Biotechnology, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/978-1-61779-352-3_2DOI Listing
February 2012

k(L)a as a predictor for successful probe-independent mammalian cell bioprocesses in orbitally shaken bioreactors.

N Biotechnol 2012 Feb 26;29(3):387-94. Epub 2011 Nov 26.

Laboratory of Cellular Biotechnology, Ecole Polytechnique Fédérale de Lausanne, 1015 Lausanne, Switzerland.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.nbt.2011.10.010DOI Listing
February 2012

The PiggyBac transposon enhances the frequency of CHO stable cell line generation and yields recombinant lines with superior productivity and stability.

Biotechnol Bioeng 2011 Sep 25;108(9):2141-50. Epub 2011 Apr 25.

École Polytechnique Fédérale de Lausanne (EPFL), Laboratory of Cellular Biotechnology, CH J2-506, Station 6, CH-1015 Lausanne, Switzerland.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/bit.23167DOI Listing
September 2011

First CHO genome.

Nat Biotechnol 2011 Aug 5;29(8):718-20. Epub 2011 Aug 5.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1038/nbt.1943DOI Listing
August 2011

Efficient and reproducible mammalian cell bioprocesses without probes and controllers?

N Biotechnol 2011 Jul 19;28(4):382-90. Epub 2011 Feb 19.

Laboratory of Cellular Biotechnology, Ecole Polytechnique Fédérale de Lausanne, 1015 Lausanne, Switzerland.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.nbt.2011.02.004DOI Listing
July 2011

TubeSpin bioreactor 50 for the high-density cultivation of Sf-9 insect cells in suspension.

Biotechnol Lett 2011 May 26;33(5):897-902. Epub 2011 Jan 26.

Laboratory of Cellular Biotechnology, Institute of Bioengineering, École Polytechnique Fédérale de Lausanne, CH J2 506, Station 6, 1015 Lausanne, Switzerland.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10529-011-0527-6DOI Listing
May 2011

A simple high-yielding process for transient gene expression in CHO cells.

J Biotechnol 2011 Apr 15;153(1-2):22-6. Epub 2011 Mar 15.

Laboratory for Cellular Biotechnology (LBTC), École Polytechnique Fédéral de Lausanne (EPFL), CH-1015 Lausanne, Switzerland.

View Article

Download full-text PDF

Source
http://ac.els-cdn.com/S016816561100112X/1-s2.0-S016816561100
Web Search
http://linkinghub.elsevier.com/retrieve/pii/S016816561100112
Publisher Site
http://dx.doi.org/10.1016/j.jbiotec.2011.03.001DOI Listing
April 2011

WITHDRAWN: Cognitive behaviour therapy versus other psychosocial treatments for schizophrenia.

Cochrane Database Syst Rev 2011 Apr 13(4):CD000524. Epub 2011 Apr 13.

School of Psychology, University of Birmingham, Edgbaston, Birmingham, UK, B15 2TT.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/14651858.CD000524.pub3DOI Listing
April 2011

Generation of stable, high-producing CHO cell lines by lentiviral vector-mediated gene transfer in serum-free suspension culture.

Biotechnol Bioeng 2011 Mar 23;108(3):600-10. Epub 2010 Nov 23.

Ecole Polytechnique Fédérale de Lausanne (EPFL), Laboratory of Cellular Biotechnology, Lausanne, Switzerland.

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1002/bit.22968
Publisher Site
http://dx.doi.org/10.1002/bit.22968DOI Listing
March 2011

Hyperosmolarity enhances transient recombinant protein yield in Chinese hamster ovary cells.

Biotechnol Lett 2010 Nov 24;32(11):1587-92. Epub 2010 Jun 24.

Laboratory of Cellular Biotechnology, Faculty of Life Sciences, École Polytechnique Fédérale de Lausanne, CH J2-506, Station 6, 1015 Lausanne, Switzerland.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10529-010-0331-8DOI Listing
November 2010

Valproic acid enhances recombinant mRNA and protein levels in transiently transfected Chinese hamster ovary cells.

J Biotechnol 2010 Jul 25;148(2-3):128-32. Epub 2010 May 25.

Ecole Polytechnique Fédérale de Lausanne, School of Life Sciences, Laboratory of Cellular Biotechnology, CH-1015 Lausanne, Switzerland.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jbiotec.2010.05.003DOI Listing
July 2010

Decennial Life Tables for the White Population of the United States, 1790-1900.

Authors:
J David Hacker

Hist Methods 2010 Apr;43(2):45-79

Binghamton University, State University of New York, Department of History, PO Box 6000, Binghamton, NY 13902-6000, 607-777-4210,

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/01615441003720449DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2885717PMC
April 2010

25 years of recombinant proteins from reactor-grown cells - where do we go from here?

Biotechnol Adv 2009 Nov-Dec;27(6):1023-1027. Epub 2009 May 20.

Ecole Polytechnique Fédérale de Lausanne (EPFL), Laboratory of Cellular Biotechnology, CH-1015 Lausanne, Switzerland; ExcellGene SA, CH-1870 Monthey, Switzerland. Electronic address:

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biotechadv.2009.05.008DOI Listing
January 2010

Respiratory syncytial virus subunit vaccine based on a recombinant fusion protein expressed transiently in mammalian cells.

Vaccine 2009 Oct 24;27(46):6415-9. Epub 2009 Jun 24.

Ecole Polytechnique Fédérale de Lausanne (EPFL), Institute of Bioengineering, Laboratory of Cellular Biotechnology, CH-1015 Lausanne, Switzerland.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.vaccine.2009.06.019DOI Listing
October 2009

DNA delivery by microinjection for the generation of recombinant mammalian cell lines.

Methods Mol Biol 2009 ;518:99-112

Ecole Polytechnique Féderale de Lausanne, EPFL-SV-IBI-LBTC, Lausanne, Switzerland.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/978-1-59745-202-1_8DOI Listing
February 2009

Calcium phosphate transfection generates mammalian recombinant cell lines with higher specific productivity than polyfection.

Biotechnol Bioeng 2008 Dec;101(5):937-45

Ecole Polytechnique Fédérale de Lausanne (EPFL), School of Life Sciences, Laboratory of Cellular Biotechnology, CHJ2-506, Station 6, CH-1015 Lausanne, Switzerland.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/bit.21972DOI Listing
December 2008

The kinetics of polyethylenimine-mediated transfection in suspension cultures of Chinese hamster ovary cells.

Mol Biotechnol 2008 Oct 10;40(2):136-43. Epub 2008 Jun 10.

Laboratory of Cellular Biotechnology, Institute of Bioengineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12033-008-9069-0DOI Listing
October 2008

Valproic acid: a viable alternative to sodium butyrate for enhancing protein expression in mammalian cell cultures.

Biotechnol Bioeng 2008 Sep;101(1):182-9

Laboratory of Cellular Biotechnology, Institute of Bioengineering, Faculty of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/bit.21882DOI Listing
September 2008

Acting on voices: Omnipotence, sources of threat, and safety-seeking behaviours.

Br J Clin Psychol 2008 Jun 23;47(Pt 2):201-13. Epub 2007 Oct 23.

Clinical Psychology Department, Birmingham and Solihull Mental Health NHS Trust, Birmingham, UK.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1348/014466507X249093DOI Listing
June 2008

Mild hypothermia improves transient gene expression yields several fold in Chinese hamster ovary cells.

Biotechnol Prog 2008 Mar-Apr;24(2):458-65. Epub 2008 Jan 26.

Ecole Polytechnique Fédérale de Lausanne, School of Life Sciences, Laboratory of Cellular Biotechnology, Lausanne, Switzerland.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1021/bp070286cDOI Listing
June 2008

Shaken helical track bioreactors: Providing oxygen to high-density cultures of mammalian cells at volumes up to 1000 L by surface aeration with air.

N Biotechnol 2008 Jun 19;25(1):68-75. Epub 2008 May 19.

Ecole Polytechnique Fédérale de Lausanne, Faculty of Life Sciences, Laboratory of Cellular Biotechnology, CH J2-506, Station 6, CH-1015 Lausanne, Switzerland.

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S18716784080000
Publisher Site
http://dx.doi.org/10.1016/j.nbt.2008.03.001DOI Listing
June 2008

Novel orbital shake bioreactors for transient production of CHO derived IgGs.

Biotechnol Prog 2007 Nov-Dec;23(6):1340-6. Epub 2007 Oct 3.

Laboratory of Cellular Biotechnology, Faculty of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland.

View Article

Download full-text PDF

Source
http://www.tpp.ch/page/downloads/TubeSpin/Stettler_Novel-Orb
Web Search
http://pubs.acs.org/cgi-bin/doilookup/?10.1021/bp070219i
Publisher Site
http://dx.doi.org/10.1021/bp070219iDOI Listing
March 2008

Recombinant protein production by large-scale transient gene expression in mammalian cells: state of the art and future perspectives.

Biotechnol Lett 2007 May 19;29(5):677-84. Epub 2007 Jan 19.

Laboratory of Cellular Biotechnology, Ecole Polytechnique Fédérale de Lausanne, Station 6, 1015, Lausanne, Switzerland.

View Article

Download full-text PDF

Source
http://link.springer.com/10.1007/s10529-006-9297-y
Publisher Site
http://dx.doi.org/10.1007/s10529-006-9297-yDOI Listing
May 2007

Scalable transient gene expression in Chinese hamster ovary cells in instrumented and non-instrumented cultivation systems.

Biotechnol Lett 2007 May 20;29(5):703-11. Epub 2007 Feb 20.

Ecole Polytechnique Fédérale de Lausanne (EPFL), Institute of Bioengineering, Laboratory of Cellular Biotechnology, 1015, Lausanne, Switzerland.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10529-006-9298-xDOI Listing
May 2007

New disposable tubes for rapid and precise biomass assessment for suspension cultures of mammalian cells.

Biotechnol Bioeng 2006 Dec;95(6):1228-33

Ecole Polytechnique Fédérale de Lausanne, Faculty of Life Sciences, Institute of Bioengineering, Laboratory of Cellular Biotechnology, CH-1015 Lausanne, Switzerland.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/bit.21071DOI Listing
December 2006

Multiple glycerol shocks increase the calcium phosphate transfection of non-synchronized CHO cells.

Biotechnol Lett 2006 Nov 29;28(22):1827-33. Epub 2006 Sep 29.

Institute of Biochemistry, Chemin des Boveresses 155, 1066, Epalinges, Switzerland.

View Article

Download full-text PDF

Source
http://link.springer.com/10.1007/s10529-006-9167-7
Publisher Site
http://dx.doi.org/10.1007/s10529-006-9167-7DOI Listing
November 2006

Disassembly of polyethylenimine-DNA particles in vitro: implications for polyethylenimine-mediated DNA delivery.

J Control Release 2006 Nov 22;116(1):96-104. Epub 2006 Sep 22.

Laboratory of Cellular Biotechnology, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH 1015 Lausanne, Switzerland.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jconrel.2006.09.006DOI Listing
November 2006

Polyethylenimine-based quality control assay for plasmid DNA.

Anal Biochem 2006 Sep 19;356(2):309-11. Epub 2006 May 19.

Laboratory of Cellular Biotechnology, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ab.2006.05.002DOI Listing
September 2006

The CELO adenovirus Gam1 protein enhances transient and stable recombinant protein expression in Chinese hamster ovary cells.

J Biotechnol 2005 Apr;117(1):21-9

Ecole Polytechnique Fédérale de Lausanne (EPFL), Laboratory of Cellular Biotechnology, CH-1015 Lausanne, Switzerland.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jbiotec.2005.01.006DOI Listing
April 2005

Orbital shaker technology for the cultivation of mammalian cells in suspension.

Biotechnol Bioeng 2005 Feb;89(4):400-6

Swiss Federal Institute of Technology, Institute of Biological Engineering and Biotechnology, Laboratory of Cellular Biotechnology, Lausanne, Switzerland.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/bit.20358DOI Listing
February 2005

Low-temperature pausing of cultivated mammalian cells.

Biotechnol Bioeng 2005 Jan;89(2):157-63

Swiss Federal Institute of Technology Lausanne, Faculty of Basic Sciences, Institute of Biological and Chemical Process Sciences, CH1015 Lausanne, Switzerland.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/bit.20320DOI Listing
January 2005

Reduction of adenovirus E1A mRNA by RNAi results in enhanced recombinant protein expression in transiently transfected HEK293 cells.

Gene 2004 Oct;341:227-34

Laboratory of Cellular Biotechnology, Swiss Federal Institute of Technology Lausanne (EPFL), CH1015 Lausanne, Switzerland.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.gene.2004.06.054DOI Listing
October 2004

Rethinking the "early" decline of marital fertility in the United States.

Authors:
J David Hacker

Demography 2003 Nov;40(4):605-20

Department of History, Binghamton University, SUNY, PO Box 6000, Binghamton, NY 13902, USA.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1353/dem.2003.0035DOI Listing
November 2003

Parotid gland tumours in 255 consecutive patients: Mount Sinai Hospital's quality assurance review.

J Otolaryngol 2002 Dec;31(6):351-4

Department of Otolaryngology, North York General Hospital, Toronto, Ontario.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2310/7070.2002.34394DOI Listing
December 2002

Can doctors be deaf to those begging to die?

Authors:
David W Hacker

Natl Obs 1974 Sep:10

View Article

Download full-text PDF

Source
September 1974