Dashuang Shi, PhD - The George Washington University - Associate Professor

Dashuang Shi

PhD

The George Washington University

Associate Professor

United States

ORCID logohttps://orcid.org/0000-0002-5491-1104

Dashuang Shi, PhD - The George Washington University - Associate Professor

Dashuang Shi

PhD
Introduction

Primary Affiliation: The George Washington University - United States

Metrics

48

Publications

255

Profile Views

141

Reads

416

PubMed Central Citations

Education
Jul 1997
University of Sydney
PhD
Apr 1987
Xiamen University
MS
Jul 1983
Xiamen University
BS
Top co-authors
Mendel Tuchman
Mendel Tuchman

Children's National Medical Center

30
Norma M Allewell
Norma M Allewell

The George Washington University

24
Hiroki Morizono
Hiroki Morizono

Children's Research Institute

17
Xiaolin Yu
Xiaolin Yu

Zhejiang University

16
Ljubica Caldovic
Ljubica Caldovic

The George Washington University

12
Zhongmin Jin
Zhongmin Jin

Institute of Medical and Biological Engineering

9
Gengxiang Zhao
Gengxiang Zhao

Children's National Medical Center

7
Lauren Roth
Lauren Roth

University of Colorado

7
Michael H Malamy
Michael H Malamy

Tufts University School of Medicine

5
Yongdong Li
Yongdong Li

Wuhan Institute of Virology

5

Publications

48Publications

141Reads

416PubMed Central Citations

Streptococcus pneumoniae Sialidase SpNanB-Catalyzed One-Pot Multienzyme (OPME) Synthesis of 2,7-Anhydro-Sialic Acids as Selective Sialidase Inhibitors.

J Org Chem 2018 Sep 23;83(18):10798-10804. Epub 2018 Aug 23.

Department of Chemistry , University of California , One Shields Avenue , Davis , California 95616 , United States.

View Article
September 2018
5 Reads
4.72 Impact Factor

Sources and Fates of Carbamyl Phosphate: A Labile Energy-Rich Molecule with Multiple Facets.

Biology (Basel) 2018 Jun 12;7(2). Epub 2018 Jun 12.

Center for Genetic Medicine Research, Children's National Medical Center, Washington, DC 20010, USA.

View Article
June 2018
1 Read

From Genome to Structure and Back Again: A Family Portrait of the Transcarbamylases.

Int J Mol Sci 2015 Aug 12;16(8):18836-64. Epub 2015 Aug 12.

Center for Genetic Medicine Research, Children's National Medical Center, the George Washington University, Washington, DC 20010, USA.

View Article
August 2015
2 Reads
3 PubMed Central Citations(source)
2.86 Impact Factor

Expression, characterization and crystal structure of thioredoxin from Schistosoma japonicum.

Parasitology 2015 Jul 26;142(8):1044-52. Epub 2015 Mar 26.

Key Laboratory of Organo-Pharmaceutical Chemistry,Jiangxi Province,Chemistry and Chemical Engineering College,Gannan Normal University,Ganzhou 341000,People's Republic of China.

View Article
July 2015
3 Reads
2.56 Impact Factor

The N-Acetylglutamate Synthase Family: Structures, Function and Mechanisms.

Int J Mol Sci 2015 Jun 9;16(6):13004-22. Epub 2015 Jun 9.

Center for Genetic Medicine Research and Department of Integrative Systems Biology, Children's National Medical Center, the George Washington University, Washington, DC 20010, USA.

View Article
June 2015
2 Reads
2 PubMed Central Citations(source)
2.86 Impact Factor

Structures of the N-acetyltransferase domain of Xylella fastidiosa N-acetyl-L-glutamate synthase/kinase with and without a His tag bound to N-acetyl-L-glutamate.

Acta Crystallogr F Struct Biol Commun 2015 Jan 1;71(Pt 1):86-95. Epub 2015 Jan 1.

Center for Genetic Medicine Research and Department of Integrative Systems Biology, Children's National Medical Center, The George Washington University, Washington, DC 20010, USA.

View Article
January 2015
2 Reads
1 PubMed Central Citation(source)

Crystal structure of the N-acetyltransferase domain of human N-acetyl-L-glutamate synthase in complex with N-acetyl-L-glutamate provides insights into its catalytic and regulatory mechanisms.

PLoS One 2013 24;8(7):e70369. Epub 2013 Jul 24.

Center for Genetic Medicine Research and Department of Integrative Systems Biology, Children's National Medical Center, The George Washington University, Washington, DC, United States of America.

View Article
February 2014
2 Reads
6 PubMed Central Citations(source)
3.23 Impact Factor

Expression, crystallization and preliminary X-ray diffraction analysis of thioredoxin glutathione reductase from Schistosoma japonicum in complex with FAD.

Acta Crystallogr F Struct Biol Commun 2014 Jan 24;70(Pt 1):92-6. Epub 2013 Dec 24.

Key Laboratory of Organo-Pharmaceutical Chemistry, Jiangxi Province, Chemistry and Chemical Engineering College, Gannan Normal University, Ganzhou 341000, People's Republic of China.

View Article
January 2014
2 Reads

Lysine carboxylation: unveiling a spontaneous post-translational modification.

Acta Crystallogr D Biol Crystallogr 2014 Jan 24;70(Pt 1):48-57. Epub 2013 Dec 24.

Department of Bioengineering, University of Illinois at Chicago, 851 South Morgan Street, Room 218, Chicago, IL 60607, USA.

View Article
January 2014
4 PubMed Central Citations(source)
7.23 Impact Factor

Structure and function of Escherichia coli RimK, an ATP-grasp fold, L-glutamyl ligase enzyme.

Proteins 2013 Oct 22;81(10):1847-54. Epub 2013 Jul 22.

Department of Integrative Systems Biology, Center for Genetic Medicine Research, Children's National Medical Center, The George Washington University, Washington, DC, 20010.

View Article
October 2013
1 Read
6 PubMed Central Citations(source)
2.63 Impact Factor

Structure of N-acetyl-L-glutamate synthase/kinase from Maricaulis maris with the allosteric inhibitor L-arginine bound.

Biochem Biophys Res Commun 2013 Aug 10;437(4):585-90. Epub 2013 Jul 10.

Center for Genetic Medicine Research, Washington, DC 20010, USA.

View Article
August 2013
15 Reads
3 PubMed Central Citations(source)
2.30 Impact Factor

Structure of the complex of Neisseria gonorrhoeae N-acetyl-L-glutamate synthase with a bound bisubstrate analog.

Biochem Biophys Res Commun 2013 Jan 20;430(4):1253-8. Epub 2012 Dec 20.

Center for Genetic Medicine Research and Department of Integrative Systems Biology, Children's National Medical Center, The George Washington University, Washington, DC 20010, USA.

View Article
January 2013
2 Reads
2 PubMed Central Citations(source)
2.30 Impact Factor

Identification of a novel Cys146X mutation of SOD1 in familial amyotrophic lateral sclerosis by whole-exome sequencing.

Genet Med 2012 Sep 17;14(9):823-6. Epub 2012 May 17.

The Institute of Genomic Medicine, Wenzhou Medical College, Wenzhou, China.

View Article
September 2012
9 Reads
6 PubMed Central Citations(source)
7.33 Impact Factor

Analysis of large phenotypic variability of EEC and SHFM4 syndromes caused by K193E mutation of the TP63 gene.

PLoS One 2012 4;7(5):e35337. Epub 2012 May 4.

Department of Oral and Maxillofacial Surgery, School of Stomatology, the Fourth Military Medical University, Xi'an, Shaanxi Province, People's Republic of China.

View Article
September 2012
2 Reads
3 PubMed Central Citations(source)
3.23 Impact Factor

Crystal structure and biochemical properties of putrescine carbamoyltransferase from Enterococcus faecalis: Assembly, active site, and allosteric regulation.

Proteins 2012 May 13;80(5):1436-47. Epub 2012 Feb 13.

Center for Genetic Medicine Research and Department of Integrative Systems Biology, Children's National Medical Center, The George Washington University, Washington, District of Columbia 20010, USA.

View Article
May 2012
4 Reads
3 PubMed Central Citations(source)
2.63 Impact Factor

A novel N-acetylglutamate synthase architecture revealed by the crystal structure of the bifunctional enzyme from Maricaulis maris.

PLoS One 2011 12;6(12):e28825. Epub 2011 Dec 12.

Center for Genetic Medicine Research and Department of Integrative Systems Biology, Children's National Medical Center, The George Washington University, Washington, DC, United States of America.

View Article
April 2012
5 Reads
7 PubMed Central Citations(source)
3.23 Impact Factor

The ygeW encoded protein from Escherichia coli is a knotted ancestral catabolic transcarbamylase.

Proteins 2011 Jul 9;79(7):2327-34. Epub 2011 May 9.

Department of Integrative Systems Biology, Children's National Medical Center, The George Washington University, Washington, District of Columbia 20010, USA.

View Article
July 2011
6 Reads
7 PubMed Central Citations(source)
2.63 Impact Factor

Reversible post-translational carboxylation modulates the enzymatic activity of N-acetyl-L-ornithine transcarbamylase.

Biochemistry 2010 Aug;49(32):6887-95

Research Center for Genetic Medicine and Department of Integrative Systems Biology, Children's National Medical Center, The George Washington University, Washington, DC 20010, USA.

View Article
August 2010
6 Reads
4 PubMed Central Citations(source)
3.02 Impact Factor

N-acetylglutamate synthase: structure, function and defects.

Mol Genet Metab 2010 26;100 Suppl 1:S13-9. Epub 2010 Feb 26.

The Children's Research Institute, Children's National Medical Center, The George Washington University, Washington, DC 20010, USA.

View Article
July 2010
10 PubMed Central Citations(source)
2.62 Impact Factor

Phenylalanines at positions 88 and 159 of Ebolavirus envelope glycoprotein differentially impact envelope function.

Virology 2010 Jan 10;396(1):135-42. Epub 2009 Nov 10.

Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research, FDA, Bethesda, MD 20892, USA.

View Article
January 2010
1 Read
6 PubMed Central Citations(source)
3.32 Impact Factor

Mechanism of allosteric inhibition of N-acetyl-L-glutamate synthase by L-arginine.

J Biol Chem 2009 Feb 18;284(8):4873-80. Epub 2008 Dec 18.

Research Center for Genetic Medicine, Children's National Medical Center, The George Washington University, Washington, D. C. 20010, USA.

View Article
February 2009
1 Read
12 PubMed Central Citations(source)
4.57 Impact Factor

The crystal structure of N-acetyl-L-glutamate synthase from Neisseria gonorrhoeae provides insights into mechanisms of catalysis and regulation.

J Biol Chem 2008 Mar 9;283(11):7176-84. Epub 2008 Jan 9.

Children's Research Institute, Children's National Medical Center, The George Washington University, Washington, DC 20010, USA.

View Article
March 2008
3 Reads
18 PubMed Central Citations(source)
4.57 Impact Factor

A single mutation in the active site swaps the substrate specificity of N-acetyl-L-ornithine transcarbamylase and N-succinyl-L-ornithine transcarbamylase.

Protein Sci 2007 Aug 28;16(8):1689-99. Epub 2007 Jun 28.

Children's National Medical Center, The George Washington University, Washington, DC 20010, USA.

View Article
August 2007
5 Reads
8 PubMed Central Citations(source)
2.85 Impact Factor

A novel bifunctional N-acetylglutamate synthase-kinase from Xanthomonas campestris that is closely related to mammalian N-acetylglutamate synthase.

BMC Biochem 2007 Apr 10;8. Epub 2007 Apr 10.

Children's Research Institute, Children's National Medical Center, The George Washington University, Washington, DC 20010, USA.

View Article
April 2007
2 Reads
18 PubMed Central Citations(source)
1.44 Impact Factor

Structure of a novel N-acetyl-L-citrulline deacetylase from Xanthomonas campestris.

Biophys Chem 2007 Mar 5;126(1-3):86-93. Epub 2006 Jun 5.

Children's Research Institute, Children's National Medical Center, 111 Michigan Avenue, N.W., Washington, DC 20010-2970, USA.

View Article
March 2007
4 Reads
8 PubMed Central Citations(source)
1.99 Impact Factor

Expression, crystallization and preliminary crystallographic studies of a novel bifunctional N-acetylglutamate synthase/kinase from Xanthomonas campestris homologous to vertebrate N-acetylglutamate synthase.

Acta Crystallogr Sect F Struct Biol Cryst Commun 2006 Dec 30;62(Pt 12):1218-22. Epub 2006 Nov 30.

Children's National Medical Center, The George Washington University, 111 Michigan Avenue, Washington, DC 20010, USA.

View Article
December 2006
8 Reads
6 PubMed Central Citations(source)
0.57 Impact Factor

Structure and catalytic mechanism of a novel N-succinyl-L-ornithine transcarbamylase in arginine biosynthesis of Bacteroides fragilis.

J Biol Chem 2006 Jul 16;281(29):20623-31. Epub 2006 May 16.

Children's Research Institute, Children's National Medical Center, George Washington University, Washington, D. C. 20010, USA.

View Article
July 2006
3 Reads
11 PubMed Central Citations(source)
4.57 Impact Factor

Acetylornithine transcarbamylase: a novel enzyme in arginine biosynthesis.

J Bacteriol 2006 Apr;188(8):2974-82

Children's Research Institute, Children's National Medical Center, 111 Michigan Avenue NW, Washington, DC 20010, USA.

View Article
April 2006
3 Reads
14 PubMed Central Citations(source)
2.81 Impact Factor

Biochemical properties of recombinant human and mouse N-acetylglutamate synthase.

Mol Genet Metab 2006 Mar;87(3):226-32

Children's Research Institute, Children's National Medical Center, The George Washington University, Washington, DC 20010, USA.

View Article
March 2006
1 Read
14 PubMed Central Citations(source)
2.62 Impact Factor

Expression, purification, crystallization and preliminary X-ray crystallographic studies of a novel acetylcitrulline deacetylase from Xanthomonas campestris.

Acta Crystallogr Sect F Struct Biol Cryst Commun 2005 Jul 15;61(Pt 7):676-9. Epub 2005 Jun 15.

Children's National Medical Center, Washington, DC 20010, USA.

View Article
July 2005
2 Reads
8 PubMed Central Citations(source)
0.57 Impact Factor

Crystal structure of N-acetylornithine transcarbamylase from Xanthomonas campestris: a novel enzyme in a new arginine biosynthetic pathway found in several eubacteria.

J Biol Chem 2005 Apr 24;280(15):14366-9. Epub 2005 Feb 24.

Children's Research Institute, Children's National Medical Center, Washington, DC 20010, USA.

View Article
April 2005
5 Reads
15 PubMed Central Citations(source)
4.57 Impact Factor

Late onset N-acetylglutamate synthase deficiency caused by hypomorphic alleles.

Hum Mutat 2005 Mar;25(3):293-8

Children's Research Institute, Children's National Medical Center, The George Washington University, Washington, DC 20010, USA.

View Article
March 2005
11 Reads
17 PubMed Central Citations(source)
5.14 Impact Factor

Mammalian N-acetylglutamate synthase.

Mol Genet Metab 2004 Apr;81 Suppl 1:S4-11

Children's Research Institute, Children's National Medical Center, The George Washington University, 111 Michigan Ave NW, Washington, DC 20010, USA.

View Article
April 2004
1 Read
15 PubMed Central Citations(source)
2.62 Impact Factor

Cloning and expression of the human N-acetylglutamate synthase gene.

Biochem Biophys Res Commun 2002 Dec;299(4):581-6

Children's Research Institute, Children's National Medical Center, The George Washington University, 111 Michigan Ave NW, Washington, DC 20010, USA.

View Article
December 2002
3 Reads
24 PubMed Central Citations(source)
2.30 Impact Factor

Crystal structure of a transcarbamylase-like protein from the anaerobic bacterium Bacteroides fragilis at 2.0 A resolution.

J Mol Biol 2002 Jul;320(4):899-908

Children's Research Institute, Children's National Medical Center, 111 Michigan Avenue N.W., Washington, DC 20010-2970, USA.

View Article
July 2002
4 Reads
10 PubMed Central Citations(source)
4.33 Impact Factor

Identification, cloning and expression of the mouse N-acetylglutamate synthase gene.

Biochem J 2002 Jun;364(Pt 3):825-31

Children's Research Institute, Children's National Medical Center, George Washington University, 111 Michigan Ave NW, Washington, DC 20010, USA.

View Article
June 2002
3 Reads
18 PubMed Central Citations(source)
4.40 Impact Factor

The clinically variable R40H mutant ornithine carbamoyltransferase shows cytosolic degradation of the precursor protein in CHO cells.

J Inherit Metab Dis 2001 Nov;24(6):614-22

Children's Research Institute, Children's National Medical Center, The George Washington University, Washington DC 20010-2970, USA.

View Article
November 2001
1 Read
3 PubMed Central Citations(source)
3.37 Impact Factor

Crystallization and preliminary X-ray crystallographic studies of wild-type human ornithine transcarbamylase and two naturally occurring mutants at position 277.

Acta Crystallogr D Biol Crystallogr 2001 May 24;57(Pt 5):719-21. Epub 2001 Apr 24.

Children's National Medical Center, 111 Michigan Avenue, Washington, DC 20010, USA.

View Article
May 2001
1 Read
1 PubMed Central Citation(source)
7.23 Impact Factor

Human ornithine transcarbamylase: crystallographic insights into substrate recognition and conformational changes.

Biochem J 2001 Mar;354(Pt 3):501-9

Children's National Medical Center, 111 Michigan Avenue, Washington, DC 20010, USA.

View Article
March 2001
15 PubMed Central Citations(source)
4.40 Impact Factor

Quantification of benzoic, phenylacetic, and phenylbutyric acids from filter-paper blood spots by gas chromatography--mass spectrometry with stable isotope dilution.

Clin Chem 2001 Feb;47(2):351-4

Children's National Medical Center and the George Washington University, 111 Michigan Ave. NW, Washington DC 20010-2970, USA.

View Article
February 2001
2 PubMed Central Citations(source)
7.91 Impact Factor

Crystal structure of human ornithine transcarbamylase complexed with carbamoyl phosphate and L-norvaline at 1.9 A resolution.

Proteins 2000 Jun;39(4):271-7

Department of Biochemistry, University of Minnesota, St. Paul, Minnesota, USA.

View Article
June 2000
1 Read
11 PubMed Central Citations(source)
2.63 Impact Factor

Crystal structure of bullfrog M ferritin at 2.8 A resolution: analysis of subunit interactions and the binuclear metal center.

J Biol Inorg Chem 1999 Jun;4(3):243-56

Department of Biochemistry, University of Minnesota, St. Paul 55108, USA.

View Article
June 1999
33 PubMed Central Citations(source)
2.54 Impact Factor

Molecular Recognition by Ornithine and Aspartate Transcarbamylases

Acc. Chem. Res. 1999 March;32(10):885-94.

Accounts of Chemical Research

View Article
March 1999
2 Reads

Three new platinum(II)-dipeptide complexes

Journal of Inorganic Biochemistry

View Article
March 1999
2 Reads

1.85-A resolution crystal structure of human ornithine transcarbamoylase complexed with N-phosphonacetyl-L-ornithine. Catalytic mechanism and correlation with inherited deficiency.

J Biol Chem 1998 Dec;273(51):34247-54

Department of Biochemistry, University of Minnesota, St. Paul, Minnesota 55108, USA.

View Article
December 1998
5 Reads
18 PubMed Central Citations(source)
4.57 Impact Factor

Localized unfolding at the junction of three ferritin subunits. A mechanism for iron release?

J Biol Chem 1998 Jul;273(30):18685-8

Department of Biochemistry, North Carolina State University, Raleigh, North Carolina 27695-7622, USA.

View Article
July 1998
1 Read
36 PubMed Central Citations(source)
4.57 Impact Factor
Top co-authors
Mendel Tuchman
Mendel Tuchman

Children's National Medical Center

30
Norma M Allewell
Norma M Allewell

The George Washington University

24
Hiroki Morizono
Hiroki Morizono

Children's Research Institute

17
Xiaolin Yu
Xiaolin Yu

Zhejiang University

16
Ljubica Caldovic
Ljubica Caldovic

The George Washington University

12
Zhongmin Jin
Zhongmin Jin

Institute of Medical and Biological Engineering

9
Gengxiang Zhao
Gengxiang Zhao

Children's National Medical Center

7
Lauren Roth
Lauren Roth

University of Colorado

7
Michael H Malamy
Michael H Malamy

Tufts University School of Medicine

5
Yongdong Li
Yongdong Li

Wuhan Institute of Virology

5