Publications by authors named "Dara Dastan"

28 Publications

  • Page 1 of 1

Chemical composition and bioactivities of essential oils from different plant parts of Rech.f. as an endemic plant from Iran.

Nat Prod Res 2021 Feb 25:1-6. Epub 2021 Feb 25.

Department of Pharmacognosy, School of Pharmacy, Ardabil University of Medical Sciences, Ardabil, Iran.

The essential oils of leaf, flower, immature and mature fruit from (Apiaceae) which grow wildly in Iran as an endemic plant were obtained by hydro-distillation and subsequently analysed by GC-FID and GC-MS. The oils obtained in yields 0.4-4.0% (w/w) and the analysis of GC-MS/FID chromatogram was resulted in the identification of 43-47 compounds, representing 90.9 - 96.7% of the total oils. α-Pinene was the main compound in all samples. The oils exhibited mild activity against , , and with or more than 15 mm zones of growth inhibition and MIC values of 9-15 mg/ml. Essential oil of immature and mature fruits showed potent antioxidant activity with IC values of 35.4 ± 0.4 and 39.1 ± 0.4 µg/mL, respectively. So, this plant as an antibacterial and antioxidant agent can be useful in food and medicine industry.
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http://dx.doi.org/10.1080/14786419.2020.1860974DOI Listing
February 2021

Clinical and phytochemical studies of Plantago major in pressure ulcer treatment: A randomized controlled trial.

Complement Ther Clin Pract 2021 Jan 27;43:101325. Epub 2021 Jan 27.

Department of Clinical Pharmacy, School of Pharmacy, Medicinal Plants and Natural Products Research Center, Hamadan University of Medical Sciences, Hamadan, Iran. Electronic address:

Background: Plantago major L. is used by local people to improve various wounds and lesions such as pressure ulcer. In this study, the therapeutic effects of P. major topical formulation on the stage 1 pressure ulcer in patients have been investigated.

Materials And Methods: This randomized triple blind clinical trial study was performed on 130 patients. During the 14 days of study, each of the test and control groups was checked according to check list. Also the topical formulation was standardized by HPLC based on the amount of quercetin.

Results: The findings of this study indicated a significant difference in resolution of the damage between the test and control groups. Topical formulation was standardized by HPLC based on the quercetin (1.88 mg/100g) and no side effects associated with this topical formulation was found.

Conclusion: The results confirmed the traditional use of P. major in resolution of the damage. CLINICAL TRIAL ID: (IRCT201609209014N117).
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http://dx.doi.org/10.1016/j.ctcp.2021.101325DOI Listing
January 2021

Design, synthesis and biological evaluation of anticholinesterase peptides: Fragment-based vs. template-based peptide design.

Bioorg Chem 2020 12 7;105:104351. Epub 2020 Oct 7.

Department of Medicinal Chemistry, School of Pharmacy, Medicinal Plants and Natural Products Research Center, Hamadan University of Medical Sciences, Hamadan, Iran. Electronic address:

The prevalence of Alzheimer's disease (AD) has become a substantial global concern. Approved AChE inhibitors have been used for symptomatic treatment of AD. Binding of amyloid β (Aβ) to the peripheral anionic site of AChE facilitates the formation of Aβ plaques. Blocking this proposed protein-protein interaction by inhibition of the peripheral anionic site of AChE, in addition to increasing the level of ACh, reduces the Aβ aggregation and might qualify to slow down the progression of disease besides the palliative treatment. Targeting protein-protein interactions consider as one of the most challenging issues in the realm of drug design in which peptides have potentials to excel in. In the present study, we applied two virtual fragment-based and template-based approaches to design peptidic inhibitors of the PAS of AChE. Based on the in silico studies, high scored peptides p2 (WTWYGYWVW) and p10 (NHRMLTRRY) obtained from fragment-based and template-based design respectively. Regarding in vitro results, p2 (IC = 16 ± 3.2 μM) and p10 (IC = 23.6 ± 4.9 μM) showed significant AChE inhibitory effects. The molecular mechanism of inhibition studied by Lineweaver-Burk plots was mixed inhibition for both peptides. The in vitro results conformed to the in silico results and showed that both peptides occupied the CAS and PAS of AChE. The comparison of two peptide-design approaches revealed that the fragment-based design had more chemical diversity and showed priority to the template-based design. According to the obtained results, peptidic inhibitors of AChE designed by the proposed fragment-based approach might be more efficient in comparison to traditional approaches.
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http://dx.doi.org/10.1016/j.bioorg.2020.104351DOI Listing
December 2020

Biochemical and Histological Evidence on the Protective Effects of (Persian Shallot) as an Herbal Supplement in Cadmium-Induced Hepatotoxicity.

Evid Based Complement Alternat Med 2020 17;2020:7457504. Epub 2020 Jun 17.

Department of Internal Medicine and Gastroenterology, School of Medicine, Yasuj University of Medical Sciences, Yasuj, Iran.

Materials And Methods: Thirty-six male Wistar rats were divided into six groups: groups 1, 2, and 3 received vehicle, Cd (100 mg/L/day by drinking water), and extract (200 mg/kg/day; orally), respectively. Groups 4, 5, and 6 were Cd groups which were treated with extract (50, 100, and 200 mg/kg/day, respectively). After 2 weeks, liver enzymes such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) and also oxidative stress biomarkers including lipid peroxidation (LPO), total antioxidant capacity (TAC), total thiol molecule (TTM), and the histopathological changes were determined using standard procedure.

Results: The findings showed that Cd caused a remarkable rise in levels of serum hepatic enzymes such as ALT ( < 0.001), AST ( < 0.01) and ALP ( < 0.001) compared with the control group. In addition, Cd led to the decreasing of the levels of TTM ( < 0.001) and TAC ( < 0.001) and increasing of LPO ( < 0.001) in liver tissue in comparison with the control group. In this regard, remarkable vascular congestion, hepatocellular degeneration, and vacuolization were observed in hepatic tissue of Cd-treated rats. Following the administration of extract, a significant improvement was observed in the functional and oxidative stress indices of hepatic tissue alongside histopathologic changes.

Conclusion: The current study indicated that the extract might prevent hepatic oxidative injury by improving oxidant/antioxidant balance in rats exposed to Cd.
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http://dx.doi.org/10.1155/2020/7457504DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7317314PMC
June 2020

Identification and tissue-specific expression of rutin biosynthetic pathway genes in Capparis spinosa elicited with salicylic acid and methyl jasmonate.

Sci Rep 2020 06 1;10(1):8884. Epub 2020 Jun 1.

Department of Plant Breeding, Swedish University of Agricultural Sciences, Växtskyddsvägen 1, SE-230 53, Alnarp, Sweden.

Capparis spinosa is an edible medicinal plant which is considered as an excellent source of rutin. Rutin is a glycoside of the flavonoid quercetin that has been reported to have a beneficial role in controlling various diseases such as hypertension, arteriosclerosis, diabetes, and obesity. In this study, the partial cDNA of four genes involved in the rutin biosynthetic pathway including 4-coumaroyl CoA ligase (4CL), flavonoid 3'-hydroxylase (F3'H), flavonol synthase (FLS) and flavonol-3-O-glucoside L-rhamnosyltransferase (RT) were identified in C.spinosa plants for the first time. The protein sequences of these genes shared high similarity with the same proteins in other plant species. Subsequently, the expression patterns of these genes as well as rutin accumulation in C.spinosa leaves treated with different concentrations of salicylic acid (SA) and methyl jasmonate (MeJA) and also in different tissues of Caper plants treated with 100 mgL SA and 150 μM MeJA were evaluated. The expression of all four genes was clearly up-regulated and rutin contents increased in response to MeJA and SA treatments after 24 h. The highest rutin contents (5.30 mgg DW and 13.27 mgg DW), as well as the highest expression levels of all four genes, were obtained using 100 mgL SA and 150 μM MeJA, respectively. Among the different tissues, the highest rutin content was observed in young leaves treated with 150 μM MeJA, which corresponded to the expression of related genes, especially RT, as a key gene in the rutin biosynthetic pathway. These results suggest that rutin content in various tissues of C. spinosa can be enhanced to a significant extent by MeJA and SA treatments and the gene expression patterns of rutin-biosynthesis-related genes are regulated by these elicitors.
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http://dx.doi.org/10.1038/s41598-020-65815-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7264309PMC
June 2020

Aerial Parts of Peucedanum chenur Have Anti-Cancer Properties through the Induction of Apoptosis and Inhibition of Invasion in Human Colorectal Cancer Cells.

Iran Biomed J 2020 09 4;24(5):314-23. Epub 2020 Mar 4.

Research Center for Molecular Medicine and Genetics, Hamadan University of Medical Sciences, Hamadan, Iran.

Background: The Peucedanum species have many pharmacological effects due to the presence of coumarins, flavonoids, phenolic compounds, and essential fatty acids in these species. In this study, for the first time, the anticancer activity of Peucedanum chenur methanolic extract via the induction of apoptosis and inhibition of invasion in HCT-116 human colon cancer cells was investigated.

Methods: P. chenur methanolic extract effect on HCT-116 cells viability and antioxidant activity were evaluated using MTT assay, 1,1-Diphenyl-2-picrylhydrazyl, and iron chelating tests, respectively. Changes in mRNA expression level in a panel of relevant genes were assessed by the quantitative real-time PCR. Also, apoptosis was assessed by cell cycle analysis and Annexin V/PI (propidium iodide) method, and the effect on cell migration was tested using scratch test.

Results: P. chenur methanolic extract increased significantly the expression of BAX while decreased the expression of BCL-2, AKT1, FAK, RhoA, and matrix metalloproteinase (MMP) genes compared to the control group. BAX/BCL-2 ratio and apoptosis elevated, whereas cell migration reduced significantly. Besides, our extract showed an appropriate antioxidant activity.

Conclusion: P. chenur may be introduced as a new chemopreventive agent in medicine due to its notable power in terms of induction of apoptosis and inhibition of invasion.
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http://dx.doi.org/10.29252/ibj.24.5.309DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7392135PMC
September 2020

Antihyperlipidemic and Antioxidative Properties of subsp. in Streptozotocin-Induced Diabetic Mice.

Diabetes Metab Syndr Obes 2020 20;13:1231-1236. Epub 2020 Apr 20.

Nutritional Sciences Department, Research Center for Environmental Determinants of Health (RCEDH), Health Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran.

Introduction: subsp. is an important food source and a well-known medicinal plant in the Zagros Mountains of Iran. The present study aimed to investigate the effect of extract and essential oil in streptozotocin-induced diabetic mice.

Materials And Methods: Different doses of hydroalcoholic extract and essential oil of subsp. (50, 100, and 200 mg/kg) were given to streptozotocin-induced diabetic mice in separate groups for three weeks. At the end of treatment, blood samples were collected; then, oxidative stress markers, TNF-α, and lipid profile were determined in its serum samples.

Results: Our findings showed that the administration of extract for three consecutive weeks significantly improved the lipid profile, oxidative stress, and inflammation process by reducing lipid peroxidation and increasing total antioxidant capacity.

Conclusion: This study showed that subsp. has antioxidant and blood lipid-lowering effects that can be used as a supplement to improve diabetes complications.
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http://dx.doi.org/10.2147/DMSO.S250417DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7182454PMC
April 2020

To be ionized or not to be ionized: the vital role of physicochemical properties of galbanic acid derivatives in AChE assay.

J Biomol Struct Dyn 2020 May 15:1-9. Epub 2020 May 15.

Department of Pharmacognosy, School of Pharmacy, Medicinal Plants and Natural Products Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.

Alzheimer's disease is a progressive neurodegenerative disorder and patients suffer from memory loss, a decline in language skill and impairment in other cognitive functions. In the cholinergic hypothesis, dysfunction of cholinergic neurons especially in the hippocampus and cerebral cortex contributes to cognitive decline in patients. So agents that enhance acetylcholine concentration could improve cognitive function. AChEIs are among the most studied anti-Alzheimer agents. Galbanic acid as a natural compound with a sesquiterpene coumarin scaffold is a weak inhibitor of AChE. In the present contribution, we discussed the impact of carboxylic group ionization on inhibitory effects. We performed and studies on galbanic acid, methyl and ethyl galbanates as AChE inhibitors. The order of inhibitory effect on AChE was obtained as ethyl galbanate ∼ methyl galbanate > galbanic acid. Our study highlights the important role of the physicochemical properties of natural lead compounds in each specific assay.Communicated by Ramaswamy H. Sarma.
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http://dx.doi.org/10.1080/07391102.2020.1764391DOI Listing
May 2020

Phytochemical, antioxidant, enzyme activity and antifungal properties of and explants stimulated by some chemical elicitors.

Pharm Biol 2020 Dec;58(1):286-296

Department of Biochemistry and Biotechnology, University of Thessaly, Larissa, Greece.

Jamzad. (Lamiaceae), is known for its antifungal and antioxidant compounds, especially rosmarinic acid (RA). The study examines the effect of elicitors on RA production and phytochemical properties of plants were treated with methyl jasmonate (MeJA) and multi-walled carbon nanotubes (MWCNTs). plants were treated with MWCNTs and salicylic acid (SA). RA was measured by HPLC. Catalase (CAT), guaiacol peroxidase (POD) and ascorbate peroxidase (APX) were quantified. DPPH and β-carotene were assayed in extracts. The antifungal effects of extracts were evaluated against K FsK). The highest RA contents of plants were 50 mg/L MeJA (140.99 mg/g DW) and 250 mg/L MWCNTs (140.49 mg/g DW). The highest were 24 h MWCNTs (7.13 mg/g DW) and 72 h SA (9.12 mg/g DW). The maximum POD and APX activities were at 100 mg/L MeJA (5 and 4 mg protein, respectively). CAT had the highest activities at 50 mg/L MeJA (2 mg protein). DPPH and β-carotene showed 50% and 80% inhibition, respectively. The FsK aggregation was the lowest for extract in number of conidia [1.82 × 10], fresh weight (6.51 g) and dry weight (0.21 g) that proved RA inhibitory effects. The callus reduces FsK growth diameter to 2.75 on the 5th day. Application of MeJA, SA, and MWCNTSs could increase RA in and highlighted potential characteristics in pharmaceutical and antifungal effects.
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http://dx.doi.org/10.1080/13880209.2020.1743324DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7178849PMC
December 2020

In Vitro Activity of and Its Main Essential Oil Component Trans-Anethole on .

Iran J Parasitol 2019 Oct-Dec;14(4):631-638

Department of Medical Parasitology and Mycology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.

Background: Trichomoniasis is one of the most common nonviral sexually transmitted infections worldwide which drug-resistant cases of the infection are rising. The aim of the study was to assessment the in vitro activity of and its main essential oil component on . Also phytochemical investigation of essential oil was performed.

Methods: Five isolates subjected to susceptibility testing against essential oil and extracts of and anethole using microtiter plate method. The minimum lethal concentration (MLC) of the natural products was assessed in comparison with metronidazole. Gas chromatography-mass spectrometry and gas chromatography-flame ionization detector was applied for chemical investigation of the essential oil.

Results: After 48 hours incubation, the most potent antitrichomonal agents were the methanolic and hexanic extract with MLC of 360 μg/ml and followed by the essential oil and anethole (1600 μg/ml). The isolates were sensitive to metronidazole with a mean MLC of 13.7 μg/ml. E-Anethole (88.41 %) was the major constituent of essential oil.

Conclusion: The results suggested in vitro antiprotozoal properties of and anethole against . Therefore further studies are needed to evaluate their in vivo effects and toxicity.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7028236PMC
February 2020

Hepatoprotective potential and antioxidant activity of in acetaminophen-induced oxidative damage.

Res Pharm Sci 2019 Dec 11;14(6):488-495. Epub 2019 Dec 11.

Medicinal Plants and Natural Products Research Center, Hamadan University of Medical Sciences, Hamadan, I.R. Iran.

() is a species of wild native to northwest Iran that its hepatoprotective effects have not yet been confirmed. This study investigated the effect of plant against acetaminophen (APAP)-induced acute liver damage. After preliminary studies, the methanol fraction (ATMF) was selected for study. Thirty-six rats were divided into six groups of 6 each and treated by gavage as follows: groups 1 and 2 received normal saline; group 3 received 400 mg/kg of ATMF; and groups 4-6 were treated with 100, 200, and 400 mg/kg of ATMF, respectively. After two consecutive weeks, except groups 1 and 3, rats were administered with an oral single dose of APAP (2 g/kg). After 48 h, blood and liver samples were collected for histological and biochemical examinations. The results showed that APAP caused a significant increase in alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase and alkaline phosphatase serum levels, lipid peroxidation (all with < 0.001) and hepatic nitric oxide ( < 0.01). In addition, APAP led to the depletion of the total antioxidant capacity, total thiol group (both with < 0.001), and structural alterations in the hepatic tissue. Following administration of ATMF extract, a significant improvement was observed in the functional and oxidative stress markers of hepatic tissue alongside histopathologic changes. In conclusion, the present study showed that the administration of ATMF might prevent hepatic oxidative damage by improving oxidant/antioxidant balance in animals exposed to APAP.
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http://dx.doi.org/10.4103/1735-5362.272535DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6937746PMC
December 2019

Extract Improve Acetaminophen-Induced Hepatic Failure through Inhibition of Oxidative/Nitrosative Stress.

J Pharmacopuncture 2019 Dec 31;22(4):239-247. Epub 2019 Dec 31.

Medicinal Plants and Natural Products Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.

Objectives: Allium jesdianum (Aj) is a medicinal plant that has highlighted pharmacological features. In this study, the effects of Aj extract were examined on acetaminophen (APAP)-induced hepatic failure in rats.

Methods: Methanolic fraction of hydro-alcoholic extract of Aj was obtained by silica gel column chromatography method. Animals were randomly divided into four groups each containing six rats and treated by gavage as follows: the first and second groups received normal saline, the third and fourth groups were received with 50 and 100 mg/kg of Aj extract, respectively. After two consecutive weeks, the groups 2-4 were given a single dose of APAP (2 g/kg). After 48 hours, blood and liver samples were collected for biochemical and histological examinations.

Results: The findings of the study demonstrated that APAP caused a significant increase in ALT (P < 0.001), AST (P < 0.001), LDH (P < 0.001), ALP (P < 0.001) serum levels, hepatic lipid peroxidation (LPO; P < 0.001) and nitric oxide (NO; P < 0.001). In this regard, APAP led to the depletion of the total antioxidant capacity (TAC; P < 0.001), glutathione and total thiol groups (TTGs; P < 0.001), and structural change in the liver. In the Aj extract groups, a considerable improvement was found in the hepatic function alongside the histopathologic changes.

Conclusion: This investigation indicated that the influential effects of Aj extract in APAP-induced hepatic failure might depend on its effect on improving oxidant/antioxidant balance in hepatic tissue.
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http://dx.doi.org/10.3831/KPI.2019.22.032DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6970575PMC
December 2019

Investigating the effect of radiosensitizer for Ursolic Acid and Kamolonol Acetate ‌ on HCT-116 cell line.

Bioorg Med Chem 2020 01 16;28(1):115152. Epub 2019 Oct 16.

Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran. Electronic address:

Purpose: The aim of this study was evaluating the cytotoxic and radiosensitizing effects of Ursolic Acid (UA) and Kamolonol Acetate (KA) on HCT116 cell line and finally investigating the functional role of NF-κB and CCND1 genes in the radiosensitizing activity of UA and KA.

Materials And Method: The cytotoxic effects of UA and KA by MTT assay was evaluated on HCT-116. Clonogenic assay was performed to investigate of radiosensitizing effects of UA and KA on HCT116. To assessment the expression levels of NF-κB and CCND1 genes, real-time PCR method was used.

Results: The results of MTT assay revealed that UA and KA have cytotoxic effects on HCT116 cell line. According to clonogenic assay, survival fraction of treated cells with UA and KA has been decreased compared to the survival fraction of untreated cells. UA and KA lead to the decrease in the expression level of NF-κB. Synergistic effect of radiosensitizing agents with radiation was only approved for UA and 2 Gy of radiation.

Conclusion: Based on our study, UA and KA have cytotoxic effects on HCT116 cell line. Furthermore, UA may lead to radiosensitization of human colorectal tumor cells by NF-κB1 and CCND1signaling pathways.
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http://dx.doi.org/10.1016/j.bmc.2019.115152DOI Listing
January 2020

Molecular characterization of clinical and environmental isolated in a burn center.

Saudi J Biol Sci 2019 Nov 20;26(7):1731-1736. Epub 2018 Jul 20.

Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.

In burn centers, acts as a major cause of nosocomial infections. Therefore, this study aimed to characterize molecularly isolates collected from environmental samples and burn patients. A total of 78 strains (including 58 clinical and 20 environmental isolates) of the were collected from Beasat hospital of Hamadan, west of Iran, and was identified using API 20NE. The disk diffusion method according to the CLSI was applied for determination of the antimicrobial resistance. Moreover, the microtiter plate test was used for the quantification of Biofilm formation. The genomic features of the isolated strains was evaluated using Pulsed Field Gel Electrophoresis (PFGE). We found that 94.8% of clinical and 80% environmental isolates were capable of forming biofilm. The rate of MDR in clinical and environmental isolates was 51.7% and 40%, respectively. A significant relationship was observed between biofilm formation capability and multiple drug resistance (). PFGE typing showed 11 different clusters with two major clusters A with 30 (38.5%) and B with 14 (17.9%) members, containing up to 56.4% of all isolates. There was no relationship between biofilm formation ability and antibiotic resistance patterns with PFGE patterns. According to the results, the clonal spread of environmental isolates is associated with clinical isolates, and both environmental and clinical isolates are attributed to a high prevalence of the antibiotic resistance and biofilm formation ability. This study highlighted that the prevention programs should be implemented in the hospital environment to control the spread of in burn units.
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http://dx.doi.org/10.1016/j.sjbs.2018.07.009DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6864211PMC
November 2019

The cytotoxicity of malathion and essential oil of (lamiales: lamiaceae) against vertebrate and invertebrate cell lines.

Pan Afr Med J 2019 6;33:285. Epub 2019 Aug 6.

Medicinal Plants and Natural Products Research Center, School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran.

Introduction: Pesticides are used as essential tools to control vector-borne diseases and agricultural pests and maintain quality and quantity crop production. Scientists attempt to use derived plant natural products due to environmental safety and low mammalian toxicity. Therefore, the cytotoxicity of malathion and essential oil against vertebrate L929 and invertebrate Sf9 cell lines were investigated.

Methods: About 2×10 cells were placed into the wells of a 96-well plate experiments. Then appropriate concentrations of malathion and essential oil added to the wells. The cells were allowed to grow for 3-5 days and estimated the cell numbers. Control cell wells contained only cells with DMSO. All treatments and controls repeated at least four replicates.

Results: About 2×10 cells were placed into the wells of a 96-well plate experiments. Then appropriate concentrations of malathion and essential oil added to the wells. The cells were allowed to grow for 3-5 days and estimated the cell numbers. Control cell wells contained only cells with DMSO. All treatments and controls repeated at least four replicates.

Conclusion: Plant essential oil not only had no negative effects but also had boosting effects on the L929 cell viability. essential oil had negative effects on the Sf9 cell viability with the differences that derived plant natural products containing environmentally friendly and readily biodegradable derivatives, hydrolyzing rapidly in nature and nearly having no destructive effects on mammals and environment.
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http://dx.doi.org/10.11604/pamj.2019.33.285.18776DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6815490PMC
November 2019

Hairy root induction and Farnesiferol B production of endemic medicinal plant .

3 Biotech 2019 Nov 22;9(11):407. Epub 2019 Oct 22.

1Department of Agronomy and Plant Breeding, University of Kurdistan, 416, Sanandaj, Iran.

The effects of medium, gibberellic acid (GA) and stratification treatments on the seed germination of were evaluated. Filter paper medium, 500 micro molar GA and 8 week chilling treatment were resulted in significantly more seed germination than others. was also transformed by . Explants from young leaves, stems, cotyledon, and embryo were inoculated with strains ATCC 15834, 1724, A4, LB9402 and Ar318. Hairy roots were induced only from 10 to 12-days embryo explants using strains ATCC 15824 and 1724. Although, the transformation efficiency of ATCC 15834 (4%) strain was higher than 1724 (2%). Maximum hairy root transformation frequency (25%) was obtained in infection time of 10 min compared to that of 20 (20%) and 30 (5%) min. In addition, the transformation rate was significantly higher at the inoculation time of 72 h (29%) compared to that of 48 h (22%) and 24 h (6%). Transgenic hairy root lines were confirmed by PCR amplification of B gene. Hairy root lines were produced higher biomass in half B5 medium compared to that of half MS medium. Hairy roots lines from the strain ATCC 15834 produced more hairy root numbers and fresh and dried biomass compared to that of the strain 1724. Analyses of transgenic hairy root and natural roots extracts using HPLC showed that all the hairy root lines produced farnesiferol B.
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http://dx.doi.org/10.1007/s13205-019-1935-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6805845PMC
November 2019

The spatio-temporal expression of some genes involved in the biosynthetic pathways of terpenes/phenylpropanoids in yarrow (Achillea millefolium).

Plant Physiol Biochem 2019 Sep 29;142:43-52. Epub 2019 Jun 29.

Department of Agronomy and Plant Breeding, University of Kurdistan, Sanandaj, Iran.

Yarrow (Achillea millefolium) is a medicinal plant from the Asteracea which biosynthesize different secondary metabolites especially terpenes and phenylpropanoids. To improve our understanding of the regulatory mechanisms behind the biosynthesis of these compounds we analyzed the expression of some genes associated with the biosynthesis of terpenes and phenylpropanoids in different tissues and in response to trans-cinnamic acid (tCA) as an inhibitor of PAL activity. Isolation and expression analysis of DXR, GPPS, PAL and CHS genes together with linalool synthase (LIS) as monoterpene synthase was conducted in different developmental stages of leaves, flowers and in response to trans-cinnamic acid (tCA). Differential expression of these genes observed in different tissues. tCA up-regulated the biosynthetic genes of monterpenes and down-regulated the biosynthetic genes of phenylpropanoids. Gene expression analysis in intact leaves and leaves without glandular trichomes showed that DXR, LIS, PAL and CHS are highly expressed in glandular trichomes while GPPS expressed ubiquitously. Analysis of essential oils composition showed that sesquiterpenes and monoterpenes are main compounds; in which from 57 identified compounds the highest were germacreneD (% 11.5), guaiol (%10.38), spatulenol (%8.73) and caryophyllene oxide (%7.48).
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http://dx.doi.org/10.1016/j.plaphy.2019.06.036DOI Listing
September 2019

Protective effects of Allium hirtifolium Boiss extract on cadmium-induced renal failure in rats.

Environ Sci Pollut Res Int 2019 Jun 10;26(18):18886-18892. Epub 2019 May 10.

Medicinal Plants and Natural Products Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.

Cadmium (Cd), as a toxic metal, can accumulate in kidneys and induce renal failure. This study was undertaken to evaluate the protective effects of Allium hirtifolium Boiss bulbs against Cd-induced renal failure in rats. Thirty-six rats were divided into 6 groups: group 1, 2, and 3 received vehicle, Cd (100 mg/L/day by drinking water), and AhB extract (200 mg/kg/day; orally), respectively. Groups 4, 5, and 6 were Cd groups which treated AhB extract (50, 100, and 200 mg/kg/day, respectively). After 2 weeks, renal function and oxidative stress markers were determined by using colorimetric methods. Our findings showed that Cd caused a significant increase in creatinine (Cr; p<0.05), uric acid (p<0.01), BUN (p<0.05), serum levels, lipid peroxidation (LPO; p<0.01), and nitric oxide (NO; p<0.01); the depletion of the total antioxidant capacity (TAC; p<0.01) and total thiol molecules (TTM; p<0.001); and structural alterations in the renal tissue. Following AhB extract administration, a remarkable improvement was observed in the functional and oxidative stress markers of renal tissue.This study suggests that AhB may prevent progression of Cd-induced renal failure via improvement of oxidative/antioxidant balance in renal tissue.
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http://dx.doi.org/10.1007/s11356-019-04656-7DOI Listing
June 2019

Clinical, in vitro and phytochemical, studies of Scrophularia striata mouthwash on chronic periodontitis disease.

J Ethnopharmacol 2019 Jul 13;239:111872. Epub 2019 Apr 13.

Medicinal Plants and Natural Products Research Center, Hamadan University of Medical Sciences, Hamadan, Iran; Department of Pharmacognosy and Pharmaceutical Biotechnology, School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran. Electronic address:

Ethnopharmacological Relevance: Chronic periodontitis is an inflammatory process of the oral cavity that is caused by different reasons. Streptococcus mutans bacteria is the main cause of the disease. Scrophularia striata in Iranian traditional medicine is used to heal inflammation of gum and mouth. Also, In vitro anti-bacterial effect of S. striata in S. mutans have been demonstrated.

Aim Of The Study: Clinical, in vitro and chemical constituent studies of S. striata on chronic periodontitis disease.

Materials And Methods: This study is a randomized clinical trial that was performed in Dental College, Hamadan University of Medical Sciences. Fifty patients with chronic periodontitis (20-50 years old) were selected and the study was approved and registered in Iranian Registry of Clinical Trials as number IRCT2015081323468N1 prior to the experiment. The 50 patients were divided into two groups. One group used herbal mouthwash that was made from hydro alcoholic extract of S. striata and another group used Irsha mouthwash (Iranian form of Listerine mouthwash). Patients were followed up after two weeks and four weeks of using mouthwash for assessment of plaque index (PI), pocket depth (PD), bleeding on probing (BOP) and counting Streptococcus mutans. The mouthwash was standardized according to gallic acid, quercetin and apigenin reference using the RP-HPLC method. The total phenolic and flavonoids contents were also determined spectrophotometerically.

Results: S. striata in the short term, improve plaque index, pocket depth and bleeding on probing but the number of Streptococcus mutans changed significantly in the long term and is more potent in comparison to Irsha mouthwash. The mouthwash was standardized according to gallic acid, quercetin and apigenin standard (3, 24, 11 μg, respectively). The total phenolic and flavonoid content of mouthwash were 120.7 μg GAE/100 cc and 78.6 μg QE/100 cc respectively.

Conclusions: S. striata mouthwash is effective in the treatment of chronic periodontitis disease and is more potent comparing the Iranian Irsha mouthwash.
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http://dx.doi.org/10.1016/j.jep.2019.111872DOI Listing
July 2019

The occurrence of acetaminophen/codeine as an adulterant in herbal analgesic supplements in Hamadan, Iran: A pilot study.

Complement Ther Med 2019 Feb 20;42:223-225. Epub 2018 Nov 20.

Medicinal Plants and Natural Products Research Center, Hamadan University of Medical Sciences, Hamadan, Iran; Department of Pharmacognosy and Pharmaceutical Biotechnology, School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran. Electronic address:

Analgesics, such as acetaminophen (APAP) and codeine (COD), are used to adulterate medicinal herbs and/or herbal supplements. This study evaluated the APAP and COD levels in 60 herbal supplement and/or herb-based medicine samples collected from apothecaries in Hamadan, Iran. The samples were analysed using a high-performance liquid chromatography (HPLC) system. The results showed that 15% of the samples contained 38900-165200 ng/g and 31.1-603.3 ng/g of APAP and COD, respectively. Due to the side-effects of analgesic drugs in human, control of these drugs is recommended in herbal supplements.
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http://dx.doi.org/10.1016/j.ctim.2018.11.018DOI Listing
February 2019

Anticancer activity, calf thymus DNA and human serum albumin binding properties of Farnesiferol C from .

J Biomol Struct Dyn 2019 Jul 22;37(11):2789-2800. Epub 2018 Oct 22.

a Research Center for Molecular Medicine , Hamadan University of Medical Sciences , Hamadan , Iran.

In this study, Farnesiferol C was introduced as an anti-colon cancer agent. Its cytotoxicity was investigated on two cancer cell lines, HCT116 and CT26, and mesenchymal stem cells (MSCs) as normal cells employing MTT assay. Moreover, Farnesiferol C interactions with ct-DNA and HSA were investigated by various techniques. The IC values of Farnesiferol C on HCT116 and CT26 cells were 42 and 46 μM, respectively, while its IC value on MSCs cells was 92 μM, indicating that Farnesiferol C was more efficacious against cancer cell lines than normal cells. DNA competitive binding studies, viscosity and zeta potential measurements confirmed that Farnesiferol C bound to DNA through intercalation binding. HSA binding investigations revealed that there were two different binding sites for Far C on HSA with higher binding affinity in site 2 compared to site 1. Furthermore, Farnesiferol C could bind to HSA and quench its intrinsic fluorescence in a static quenching mechanism, with a distance of 2.54 nm. Competitive binding in the presence of warfarin and ibuprofen was carried out and the resulting quenching constant was strongly changed in the presence of warfarin. Consequently, Farnesiferol C most probably will be located within sub-domain IIA. In this study, molecular modeling buttressed and confirmed our laboratory results. Conclusively, we proposed that DNA is an appropriate target for Farnesiferol C. Therefore, Farnesiferol C and its semisynthetic analogues can be one of the priority innovations in research on anticancer drugs.
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http://dx.doi.org/10.1080/07391102.2018.1497543DOI Listing
July 2019

Ferula pseudalliacea induces apoptosis in human colorectal cancer HCT-116 cells via mitochondria-dependent pathway.

Arch Physiol Biochem 2019 Jul 27;125(3):284-291. Epub 2018 Mar 27.

a Research Center for Molecular Medicine , Hamadan University of Medical Sciences , Hamadan , Iran.

Ferula species have diverse biological functions. This study set out to investigate the anti-proliferative effects of methanolic extract of F. pseudalliacea against human colon cancer HCT-116 cell line. Cytotoxic effects of F. pseudalliacea on HCT-116 cells was estimated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) assay. Real-time polymerase chain reaction (PCR) and Western blot were employed to analyze BAX and Bcl2 expression. Cell cycle analysis and apoptosis were conducted using flowcytometry and Annexin V/ propidium iodide (PI) staining. Rhodamine 123 staining and enzyme-linked immunosorbent assay (ELISA) assay were employed to quantify the mitochondria membrane potential (MMP) and caspase 3 activity, respectively. F. pseudalliacea markedly decreased HCT-116 cells viability. The gene and protein expression of BAX were increased, whereas Bcl-2 was decreased in F. pseudalliacea treated cells. F. pseudalliacea induced apoptosis via promotion of cell cycle arrest, caspase 3 activation, and destruction of MMP. These results demonstrate that F. pseudalliacea extract is able to induce apoptosis in HCT-116 cells mainly by activation of the mitochondrial pathway.
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http://dx.doi.org/10.1080/13813455.2018.1455710DOI Listing
July 2019

Chemical composition and biological properties of Maroofi.

J Food Sci Technol 2017 Mar 6;54(3):727-734. Epub 2017 Feb 6.

Medicinal Plants and Natural Products Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.

is an indigenous plant which is frequently used as a spice in Avraman-Kurdistan region of Iran. The present study aimed to investigate the chemical composition, antimicrobial and antioxidant properties of the . In addition, rosmarinic acid and total phenolic content of was assessed by spectrophotometric method and HPTLC. The essential oil and methanolic extract were isolated by hydrodistillation and maceration methods, respectively. A total of 32 compounds representing 98.6% of the essential oil were identified by GC-MS and GC-FID. The main constituents were -pentacosane (23.8%), spathulenol (11.5%), β-bourbonen (11.3%) and -docosane (11.0%). The antibacterial activity of samples were carried out by disc diffusion method and evaluate the minimal inhibitory concentration (MIC) essential oil and methanolic extract were found to be effective against , and . The highest scavenging activity was found for methanolic extract of (21.58 µg/mL) and the total phenolics of methanolic extract of was 95.3 mg GAE/g. The rosmarinic acid content of methanolic extract was 0.83 mg/g plant. Antioxidant activity and rosmarininc acid content of suggests that the essential oil and methanolic extract of has great potential for application as a natural antimicrobial and antioxidant agent to preserve food.
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http://dx.doi.org/10.1007/s13197-017-2512-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5334231PMC
March 2017

Chemical Composition, Antioxidant, and Antimicrobial Activities on Laserpitium carduchorum Hedge & Lamond Essential Oil and Extracts During Various Growing Stages.

Chem Biodivers 2016 Oct;13(10):1397-1403

Research Center of Agriculture and Natural Resources, Forked Road of Jame-Jam, Sanandaj, Iran.

Laserpitium carduchorum is frequently used as a spice, and in Bane folk medicine, the aerial parts of this are used to treat urinary infections. Variation in the quantity and quality of the essential oil of Iranian L. carduchorum at different developmental growth stages including vegetative, flowering, and seed ripening is reported. In total, 33 compounds were identified and quantified in the oils of vegetative, flowering, and seed ripening stages, representing 97.8%, 98.8%, and 98.7% of the oils, respectively. α-Pinene (45.1, 61.4, and 46.4%), sabinene (16.5, 10.3, and 17.5%), and limonene (6.4, 8.5, and 20.4%) were the main compounds in all samples. The antioxidant activities of different extracts of L. carduchorum at different developmental growth stages were examined by employing various established in vitro experiments including DPPH, FRAP, and TEAC assays. The amounts of total phenolics were also determined spectrophotometerically. Antimicrobial activities of different extracts and essential oils of L. carduchorum at different developmental growth stages were examined against five Gram-positive and four Gram-negative bacteria, as well as two fungi. The results showed that maximum antioxidant and antimicrobial activity of extracts were at the flowering stage of the plant. Maximum antimicrobial activity of essential oils was at seed ripening stage.
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http://dx.doi.org/10.1002/cbdv.201600087DOI Listing
October 2016

New coumarin derivatives from Ferula pseudalliacea with antibacterial activity.

Nat Prod Res 2016 Dec 8;30(24):2747-2753. Epub 2016 Mar 8.

b Department of Phytochemistry, Medicinal Plants and Drugs Research Institute , Shahid Beheshti University, G. C., Evin , Tehran , Iran.

In this study, the antibacterial activity of disesquiterpene coumarin and sesquiterpene coumarins obtained from Ferula pseudalliacea roots was evaluated by determination of minimum inhibitory concentration using the broth micro-dilution method against seven pathogenic bacterial strains (Staphylococcus aureus ATCC 25,923, vancomycin resistant clinical strain of Enterococcus faecium, Bacillus cereus PTCC 1015, Escherichia coli ATCC 25,922, Pseudomonas aeruginosa PTCC 1430, clinical strain of Klebsiella pneumoniae and a clinical strain of Helicobacter pylori). The overall inhibitory activities of the compounds were higher against Gram positive tested bacteria. Sanandajin and ethyl galbanate demonstrated significant activity against H. pylori strain, as well as S. aureus strain in concentration of 64 μg/ml. Methyl galbanate inhibited vancomycin resistant strain of E. faecium in concentration of 64 μg/ml. The results of the present investigation indicated that disesquiterpene and sesquiterpene coumarins isolated from F. pseudalliacea root extract can be considered as potent antibacterial agents for pharmaceutical and food industries.
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http://dx.doi.org/10.1080/14786419.2016.1149705DOI Listing
December 2016

Bioactive sesquiterpene coumarins from Ferula pseudalliacea.

Planta Med 2014 Aug 19;80(13):1118-23. Epub 2014 Aug 19.

Division of Pharmaceutical Biology, University of Basel, Basel, Switzerland.

One new and five known sesquiterpene coumarins were isolated from the roots of Ferula pseudalliacea. The structures were elucidated by 1D and 2D NMR, and HR-ESIMS data as 4'-hydroxy kamolonol acetate (1), kamolonol (2), szowitsiacoumarin A (3), farnesiferon B (4), farnesiferol C (5), and flabellilobin A (6). The absolute configuration of compounds 1, 2, and 4 was established by comparison of experimental and simulated electronic circular dichroism spectra using time dependence density function theory. 4'-Hydroxy kamolonol acetate and kamolonol showed antibacterial activity against Heliobacter pylori and Staphylococcus aureus at a concentration of 64 µg/mL. Kamolonol, 4'-hydroxy kamolonol acetate, and farnesiferon B displayed a cytotoxic activity in HeLa cells, with an IC50 of 3.8, 4.5, and 7.7 µM, respectively.
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http://dx.doi.org/10.1055/s-0034-1382996DOI Listing
August 2014

Disesquiterpene and sesquiterpene coumarins from Ferula pseudalliacea, and determination of their absolute configurations.

Phytochemistry 2012 Jun 20;78:170-8. Epub 2012 Mar 20.

Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G. C., Evin, Tehran, Iran.

The first disesquiterpene coumarin, sanandajin, five sesquiterpene coumarins, kamolonol acetate, fekrynol acetate, ethyl galbanate, methyl galbanate, farnesiferol B, and a sesquiterpene, aristolone, were isolated from a n-hexane extract of Ferula pseudalliacea roots. The structures were elucidated by 1D and 2D NMR, HR-ESIMS data, and kamolonol acetate was confirmed by single-crystal X-ray analysis. The absolute configuration of compounds was established by comparison of experimental and simulated ECD spectra using time dependence density function theory (TDDFT). In vitro antiplasmodial activity against Plasmodium falciparum K1 strain was determined. sanandajin, kamolonol acetate and methyl galbanate showed moderate antiplasmodial activity, with IC(50) values of 2.6, 16.1 and 7.1 μM, respectively.
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http://dx.doi.org/10.1016/j.phytochem.2012.02.016DOI Listing
June 2012

Chemical composition and antioxidant activity of the essential oil of aerial parts of Petasites albus from Iran: a good natural source of euparin.

Nat Prod Res 2012 11;26(4):291-7. Epub 2011 Jul 11.

Department of Chemistry, Faculty of Science, Shahid Beheshti University, GC, Tehran, Iran.

The chemical composition of the essential oil of Petasites albus (known as 'Baba Adam' in Iran) was investigated by capillary gas chromatography and gas chromatography-mass spectrometry for the first time. Twenty components were identified, accounting for 99.7% of the oil composition. The major compounds were euparin (73%), α-eudesmol (13.2%) and β-selinene (4.5%). Euparin, the main component of the essential oil, was isolated and characterised by spectroscopic techniques. The antioxidant activities of the essential oil and euparin were evaluated by using the 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay and are shown to exhibit a moderate antioxidant activity.
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http://dx.doi.org/10.1080/14786410903374819DOI Listing
August 2012