Publications by authors named "Dapeng Zhao"

57 Publications

urinary compatibility of Mg-Sr-Ag alloy in swine model.

Bioact Mater 2022 Jan 4;7:254-262. Epub 2021 Jun 4.

Department of Bioengineering, Bourns College of Engineering, University of California at Riverside, CA, 92521, USA.

A biodegradable metallic ureteral stent with suitable mechanical properties and antibacterial activity remains a challenge. Here we reveal the scientific significance of a biodegradable Mg-Sr-Ag alloy with a favorable combination of balanced mechanical properties, adjustable indwelling time in urinary tract and evident antibacterial activity via experiments in a swine model. Attributed to the rheo-solidification process, equiaxial microstructure and significantly refined grains (average grain size: 27.1 μm) were achieved. MgSr and MgAg were found as the primary precipitates in the matrix, due to which the alloy obtained ca. 111% increase in ultimate tensile strength in comparison to pure magnesium. Both the and results demonstrated the satisfactory biocompatibility of the alloy. Histological evaluation and bioindicators analysis suggested that there was no tissue damage, inflammation and lesions in the urinary system caused by the degradation process. The stent also improved the post-operative bladder functions viewed from the urodynamic results. Our findings highlight the potential of this alloy as antibacterial biodegradable urinary implant material.
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http://dx.doi.org/10.1016/j.bioactmat.2021.05.046DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8379362PMC
January 2022

RNA-binding protein 39: a promising therapeutic target for cancer.

Cell Death Discov 2021 Aug 13;7(1):214. Epub 2021 Aug 13.

Bio-Medical Research Canter, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, 730000, China.

RNA-binding motif protein 39 (RBM39), as a key factor in tumor-targeted mRNA and protein expression, not only plays a vital role in tumorigenesis, but also has broad development prospects in clinical treatment and drug research. Moreover, since RBM39 was identified as a target of sulfonamides, it has played a key role in the emerging field of molecule drug development. Hence, it is of great significance to study the interaction between RBM39 and tumors and the clinical application of drug-targeted therapy. In this paper, we describe the possible multi-level regulation of RBM39, including gene transcription, protein translation, and alternative splicing. Importantly, the molecular function of RBM39 as an important splicing factor in most common tumors is systematically outlined. Furthermore, we briefly introduce RBM39's tumor-targeted drug research and its clinical application, hoping to give reference significance for the molecular mechanism of RBM39 in tumors, and provide reliable ideas for in-depth research for future therapeutic strategies.
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http://dx.doi.org/10.1038/s41420-021-00598-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8363639PMC
August 2021

LncRNA CASC15 promotes the proliferation of papillary thyroid carcinoma cells by regulating the miR-7151-5p/WNT7A axis.

Pathol Res Pract 2021 Sep 21;225:153561. Epub 2021 Jul 21.

Department of Endocrinology, Qilu Hospital of Shandong University, Jinan, China. Electronic address:

Long noncoding RNAs (lncRNAs) play crucial roles in the regulation of human thyroid cancer (TC), including papillary thyroid carcinoma (PTC); PTC is the most common pathological subtype of TC. To date, the expression, function, and mechanism of the lncRNA CASC15 in PTC remain unclear. The present study results showed that CASC15 was overexpressed in PTC tissues compared with normal tissues and acted as a potent oncogene to promote the proliferation and tumorigenesis of PTC cells both in vitro and in vivo. Mechanistic studies demonstrated that CASC15 could serve as an endogenous miRNA sponge to absorb and downregulate miR-7151-5p, thereby preventing the inhibition of WNT7A during PTC progression. Furthermore, the study demonstrated that CASC15 activated the WNT/β‑catenin signaling pathway by upregulating WNT7A in PTC. Taken together, our findings identified CASC15 as a potential diagnostic marker or therapeutic target for PTC progression. DATA AVAILABILITY: Please contact the corresponding author for a data request.
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http://dx.doi.org/10.1016/j.prp.2021.153561DOI Listing
September 2021

Aberrant Bcl-x splicing in cancer: from molecular mechanism to therapeutic modulation.

J Exp Clin Cancer Res 2021 Jun 12;40(1):194. Epub 2021 Jun 12.

Department of Heavy Ion Radiation Medicine, Bio-Medical Research Center, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, 730000, China.

Bcl-x pre-mRNA splicing serves as a typical example to study the impact of alternative splicing in the modulation of cell death. Dysregulation of Bcl-x apoptotic isoforms caused by precarious equilibrium splicing is implicated in genesis and development of multiple human diseases, especially cancers. Exploring the mechanism of Bcl-x splicing and regulation has provided insight into the development of drugs that could contribute to sensitivity of cancer cells to death. On this basis, we review the multiple splicing patterns and structural characteristics of Bcl-x. Additionally, we outline the cis-regulatory elements, trans-acting factors as well as epigenetic modifications involved in the splicing regulation of Bcl-x. Furthermore, this review highlights aberrant splicing of Bcl-x involved in apoptosis evade, autophagy, metastasis, and therapy resistance of various cancer cells. Last, emphasis is given to the clinical role of targeting Bcl-x splicing correction in human cancer based on the splice-switching oligonucleotides, small molecular modulators and BH3 mimetics. Thus, it is highlighting significance of aberrant splicing isoforms of Bcl-x as targets for cancer therapy.
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http://dx.doi.org/10.1186/s13046-021-02001-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8196531PMC
June 2021

The response of macrophages and their osteogenic potential modulated by micro/nano-structured Ti surfaces.

Colloids Surf B Biointerfaces 2021 Sep 13;205:111848. Epub 2021 May 13.

State Key Laboratory of Powder Metallurgy, Central South University, Changsha, 410083, PR China. Electronic address:

Current understanding on the interactions between micro/nano-structured Ti surfaces and macrophages is still limited. In this work, TiO nano-structures were introduced onto acid-etched Ti surfaces by alkali-heat treatment, ion exchange and subsequent heat treatment. By adjusting the concentration of NaOH during alkali-heat treatment, nano-flakes, nano-flakes mixed with nano-wires or nano-wires could formed on acid-etched Ti surfaces. The micro- and micro/nano-structured Ti surfaces possessed similar surface chemical and phase compositions. In vitro results indicate that the morphology of macrophages was highly dependent on the morphological features of nano-structures. Nano-flakes and nano-wires were favorable to induce the formation of lamellipodia and filopodia, respectively. Compared to micro-structured Ti surface, micro/nano-structured Ti surfaces polarized macrophages to their M2 phenotype and enhanced the gene expressions of osteogenic growth factors in macrophages. The M2 polarized macrophages promoted the maturation of osteoblasts. Compared to that with nano-flakes or nano-wires, the surface with mixed features of nano-flakes and nano-wires exhibited stronger anti-inflammatory and osteo-immunomodulatory effects. The findings presented in the current work suggest that introducing micro/nano-topographies onto Ti-based implant surfaces is a promising strategy to modulate the inflammatory response and mediate osteogenesis.
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http://dx.doi.org/10.1016/j.colsurfb.2021.111848DOI Listing
September 2021

3D anisotropic structure of the Japan subduction zone.

Sci Adv 2021 Jan 20;7(4). Epub 2021 Jan 20.

Department of Geophysics, Graduate School of Science, Tohoku University, Sendai, Japan.

How mantle materials flow and how intraslab fabrics align in subduction zones are two essential issues for clarifying material recycling between Earth's interior and surface. Investigating seismic anisotropy is one of a few viable technologies that can directly answer these questions. However, the detailed anisotropic structure of subduction zones is still unclear. Under a general hexagonal symmetry anisotropy assumption, we develop a tomographic method to determine a high-resolution three-dimensional (3D) wave anisotropic model of the Japan subduction zone by inverting 1,184,018 travel time data of local and teleseismic events. As a result, the 3D anisotropic structure in and around the dipping Pacific slab is firstly revealed. Our results show that slab deformation plays an important role in both mantle flow and intraslab fabric, and the widely observed trench-parallel anisotropy in the forearc is related to the intraslab deformation during the outer-rise yielding of the subducting plate.
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http://dx.doi.org/10.1126/sciadv.abc9620DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7817110PMC
January 2021

Hesperidin prevents hyperglycemia in diabetic rats by activating the insulin receptor pathway.

Exp Ther Med 2021 Jan 19;21(1):53. Epub 2020 Nov 19.

The First Department of Cardiovascular, First Affiliated Hospital, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150040, P.R. China.

Diabetes, a disease with high prevalence in China, is a major risk factor of cardiovascular disease. Hesperidin is a flavanone glycoside with anti-hyperglycemic and anti-hyperlipidemic activities. Therefore, the present study aimed to investigate the potential preventive effect of hesperidin against type 2 diabetes mellitus (T2DM) using a rat model of alloxan and high fat diet (HFD)-induced insulin resistance. Male Sprague Dawley rats were orally administered with 100 mg/kg hesperidin or vehicle (sodium carboxy methyl cellulose) for 35 days. Insulin resistance was induced by feeding animals a HFD for 3 weeks (from day 7) and then with an alloxan injection on day 28. Results from the study demonstrated that hesperidin improved fasting serum glucose (from 19.8 to 10.6 mmol/l) without changing the fasting insulin level, suggesting that hesperidin prevented the development of insulin resistance and diabetes by improving insulin sensitivity. In the oral glucose tolerance test, the development of impaired glucose tolerance was also prevented by hesperidin treatment. Hesperidin was found to regulate glycolysis and gluconeogenesis by enhancing the activity of glucokinase, inducing the phosphorylation of insulin receptor (IR) and phosphoinositide-dependent kinase 1 (PDK1), while decreasing the activity of glucose-6-phosphatase and phosphoenolpyruvate carboxykinase in the liver. In a cell-based assay, hesperidin increased glucose uptake in primary rat adipocytes. Collectively, the present study identified the potent preventive effect of hesperidin against HFD-induced insulin resistance by activating the IR/PDK1 pathway. The current results may provide a potential strategy lacking sides effects to improve metabolic health and reduce risks.
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http://dx.doi.org/10.3892/etm.2020.9485DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7706385PMC
January 2021

Eye Preference for Emotional Stimuli in Sichuan Snub-Nosed Monkeys.

Folia Primatol (Basel) 2020 16;91(6):630-642. Epub 2020 Sep 16.

Shaanxi Key Laboratory for Animal Conservation, College of Life Sciences, Northwest University, Xi'an, China.

This study presents the first evidence of effects of applying both positive and negative stimuli simultaneously on visual laterality in Old World monkeys. Thirteen captive individuals of Sichuan snub-nosed monkeys (Rhinopithecus roxellana) were chosen as focal subjects in the monocular box task. In total, 4 emotional categories (the preferred, the novel, the neutral, and the fearful) of visual stimuli were applied, and eye preference was recorded when individuals looked at each stimulus through an observation hole in the box. We found evidence of visual laterality at the individual level, but not at the group level for each stimulus. For the preferred stimulus, 9 individuals showed significant right-eye preference while 4 individuals showed significant left-eye preference. For the other 3 stimuli, 7 individuals displayed significant right-eye preference while 6 individuals displayed significant left-eye preference. Totally, 11 of 13 individuals showed consistency in the visual laterality direction (7 right-eye preference and 4 left-eye preference) across the 4 stimuli. The remaining 2 individuals displayed right-eye preference for the preferred stimulus while they showed left-eye preference for the other 3 stimuli. There was no significant difference among various stimuli regarding the direction of visual laterality. However, there was a significant difference in the strength of visual laterality among various stimulus categories. The strength of visual laterality for the preferred stimulus was significantly lower than that for the other 3 stimuli. The strength of visual laterality for the fearful stimulus was significantly higher than that for the novel stimulus and the neutral stimulus. Furthermore, the looking duration for the preferred stimulus was significantly higher than that for the other 3 stimuli. The looking duration for the novel stimulus was significantly higher than that for the neutral stimulus and the fearful stimulus. The looking duration for the neutral stimulus was significantly higher than that for the fearful stimulus. Our findings indicate emotional valence of stimuli significantly influence eye looking duration and the strength of visual laterality but not for the direction of visual laterality in this species. Taken together, emotional valence of stimuli plays an important role in the eye use of R. roxellana.
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http://dx.doi.org/10.1159/000509442DOI Listing
May 2021

An in situ electrical transport measurement system under ultra-high vacuum.

Rev Sci Instrum 2020 Jun;91(6):063902

State Key Laboratory of Low Dimensional Quantum Physics, Department of Physics, Tsinghua University, Beijing 100084, China.

Low-dimensional materials exhibit exotic properties and have attracted widespread attention. However, many low-dimensional materials are highly sensitive to air, making it challenging to investigate their intrinsic properties with ex situ measurements. To overcome such challenges, here, we developed a system combined with sample growth, electrode deposition, and in situ electrical transport measurement under ultra-high vacuum condition. The in situ deposition of electrodes enables desired ohmic electrical contacts between the probes and samples, which allows continuous temperature dependent resistance (R-T) measurements. Combined with a scanning tunneling microscope, surface morphology, electronic structure, and electrical transport properties of the same sample can be systematically investigated. We demonstrate the performance of this in situ electrical transport measurement system with three-unit-cell thick FeSe films grown on Nb-doped SrTiO(001) substrates, where a low-noise R-T curve with a zero-resistance superconducting transition temperature of ∼30 K is observed.
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http://dx.doi.org/10.1063/5.0004304DOI Listing
June 2020

Oleanolic Acid Attenuates Renal Fibrosis through TGF-/Smad Pathway in a Rat Model of Unilateral Ureteral Obstruction.

Evid Based Complement Alternat Med 2020 30;2020:2085303. Epub 2020 Mar 30.

The Department of Nephropathy, First Affiliated Hospital, Heilongjiang University of Chinese Medicine, No. 26 Heping Road, Xiangfang District, Harbin, Heilongjiang 150040, China.

Renal fibrosis is a common final pathological process in the progression of kidney disease. Oleanolic acid is a bioactive pentacyclic triterpenoid and is widely found in medicinal herbs around the world. In this study, we explored the effect of oleanolic acid on renal fibrosis and the underlying molecular mechanisms by using a rat model of unilateral ureteral obstruction (UUO). Male Sprague-Dawley rats were orally administered with oleanolic acid (6 mg/kg/d) or vehicle (olive oil) for 21 days after the UUO surgery. Upon termination, urine and blood were collected for renal function analysis, and kidneys were harvested for pathological analysis by using hematoxylin-eosin and Masson trichrome staining. Changes of extracellular matrix mRNA expressions and TGF-/Smad signaling in the kidneys were also determined. As a result, oleanolic acid significantly reduced the kidney index, the level of serum creatinine and blood urea nitrogen, and the urinary level of microalbumin, 1-microglobulin, and -acetyl--glucosaminidase. Masson trichrome staining showed significantly less collagen deposition in the UUO rats with oleanolic acid treatment. Diminished mRNA expressions of collagen I, collagen III, fibronectin, and -SMA in the kidney tissues were observed after the treatment. Oleanolic acid led to decreased protein expressions of TGF-, TGF- receptor I, and TGF- receptor II, as well as the phosphorylation of Smad2. Our current study suggested that oleanolic acid could be a complementary and alternative therapy for renal fibrosis potentially by targeting the TGF-/Smad pathway.
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http://dx.doi.org/10.1155/2020/2085303DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7149435PMC
March 2020

Tomography of the source zone of the great 2011 Tohoku earthquake.

Nat Commun 2020 Mar 3;11(1):1163. Epub 2020 Mar 3.

School of Earth Sciences, Zhejiang University, Hangzhou, Zhejiang, 310027, China.

The mechanism and rupture process of the giant 2011 Tohoku-oki earthquake (Mw 9.0) are still poorly understood due to lack of permanent near-field observations. Using seismic arrival times recorded by dense seismograph networks on land and at ocean floor, we determine a detailed seismic tomography model of the megathrust zone beneath the Tohoku forearc. Our results show that the coseismic slip of the 2011 Tohoku-oki earthquake initiated at a boundary between a down-dip high-velocity anomaly and an up-dip low-velocity anomaly. The slow anomaly at shallow depths near the Japan trench may reflect low-rigidity materials that are close to the free surface, resulting in large slip and weak high-frequency radiation. Our new tomographic model can account for not only large slip near the trench but also weak high-frequency radiation from the shallow rupture areas.
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http://dx.doi.org/10.1038/s41467-020-14745-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7054414PMC
March 2020

Foam cells promote atherosclerosis progression by releasing CXCL12.

Biosci Rep 2020 01;40(1)

The Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Health, The State and Shandong Province Joint Key Laboratory of Translational Cardiovascular Medicine, Qilu Hospital of Shandong University, Jinan, China.

Background: Atherosclerosis (AS) is a chronic inflammatory disease that contributes to multiple cardiovascular diseases (CVDs), and foam cell formation plays important roles in the progression of AS. There is an urgent need to identify new molecular targets for treating AS, and thereby improve the quality of life and reduce the financial burden of individuals with CVD.

Methods: An in vitro model of AS was generated by treating THP-1 cells and human aortic vascular smooth muscle cells (HA-VSMCs) with oxidized low-density lipoproteins (ox-LDLs). HA-VSMC proliferation and foam cell formation were detected by the MTT assay and Oil Red O staining. C-X-C motif chemokine 12 (CXCL12) expression was suppressed by siRNA. An AS rat model was established by feeding rats a high-fat diet and vitamin D2 for 3 weeks. Histopathology examinations were conducted by Hematoxylin and Eosin (H&E) staining and the levels ionized calcium-binding adapter molecule 1 (IBA1) and α smooth muscle actin (α-SMA) expression were determined by ELISA assays and immunohistochemistry.

Results: An in vitro model of AS was established with THP-1 cells. CXCL12 expression in the model THP-1 cells was significantly increased when compared with its expression in control cells. Suppression of CXCL12 expression reduced the progression of AS in the cell model. Moreover, CXCL12 promoted AS in the in vivo rat model.

Conclusion: Our results suggest that CXCL12 plays an important role in promoting the progression of AS. Furthermore, inhibition of CXCL12 might suppress the development of AS by inhibiting HA-VSMC proliferation and their transformation to foam cells.
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http://dx.doi.org/10.1042/BSR20193267DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6970083PMC
January 2020

How Target Animacy Affects Manual Laterality in Hylobatidae: The First Evidence in Northern White-Cheeked Gibbons (Nomascus leucogenys).

Folia Primatol (Basel) 2020 30;91(5):445-451. Epub 2019 Oct 30.

Shaanxi Key Laboratory for Animal Conservation, College of Life Sciences, Northwest University, Xi'an, China.

Our study presents the first evidence on how target animacy impacts on manual laterality in the Hylobatidae and contributes to filling the knowledge gap between monkeys and great apes in primate evolution of emotional lateralization. Eleven captive individuals of northern white-cheeked gibbons (Nomascus leucogenys) were chosen as focal subjects. There were significantly more ambipreferent individuals than left/right-handed individuals for both inanimate (χ2(1, n = 11) = 7.364, p = 0.007) and animate (χ2(1, n = 11) = 4.455, p = 0.035) targets, meaning no significant group-level hand preference. The right hand was more frequently used than the left hand for inanimate targets whereas the left hand was more frequently used than the right hand for animate targets, although the interaction between target animacy and hand use was not significant (proportion: F1, 10 = 0.283, p = 0.607; rate: F1, 10 = 0.228, p = 0.643). Our findings in N. leucogenys could not fully support either the tool use theory or the right hemisphere hypothesis.
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http://dx.doi.org/10.1159/000503344DOI Listing
October 2020

Arc-arc collision caused the 2018 Eastern Iburi earthquake (M 6.7) in Hokkaido, Japan.

Sci Rep 2019 Sep 26;9(1):13914. Epub 2019 Sep 26.

Department of Geophysics, Graduate School of Science, Tohoku University, Sendai, 980-8578, Japan.

Inland crustal earthquakes usually occur in the brittle upper crust (0-20 km depths), but the 6 September 2018 Eastern Iburi earthquake (M 6.7) took place in southern Hokkaido with a focal depth of ~37 km, causing 41 fatalities and serious damage to the local infrastructure. The reason why this event was so deep and its causal mechanism are still unclear. In this work we study the three-dimensional P and S wave seismic attenuation (1/Q) structure in the source zone of the 2018 Iburi earthquake. Our results show that this event occurred at the boundary between the Sorachi-Yezo belt (low Q) and the dipping Northeastern (NE) Japan arc (high Q) that is descending beneath the Kuril arc. The collision between the NE Japan and Kuril arcs as well as fluids from dehydration of the subducting Pacific plate caused this big event and its unusual focal depth. Similar attenuation structures are revealed in source zones of the 1970 Hidaka earthquake (M 6.7) and the 1982 Urakawa-oki earthquake (M 7.1), suggesting that they were caused by similar processes. We think that large earthquakes will take place again on the active thrust faults in southern Hokkaido in the coming decades. Hence, we should pay much attention to the seismic risk and prepare for reduction of earthquake hazards there.
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http://dx.doi.org/10.1038/s41598-019-50305-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6763458PMC
September 2019

Rubidium-containing mesoporous bioactive glass scaffolds support angiogenesis, osteogenesis and antibacterial activity.

Mater Sci Eng C Mater Biol Appl 2019 Dec 1;105:110155. Epub 2019 Sep 1.

Department of Materials Engineering, Monash University, Victoria 3800, Australia.

In this study, rubidium-containing mesoporous bioglass (Rb-MBG) scaffolds were formed with the investigation of the influence of Rb addition on angiogenic and osteogenic differentiation abilities of hBMSC. The phase composition, microstructure, pore size distribution, ion release, biological activity, drug loading rate, and release rate of Rb-MBG were characterized. The proliferation and differentiation of hBMSC, the markers of bone formation (ALP, COL-1) and angiogenesis (VEGF, HIF-1α), and wnt/β-catenin related-signaling pathway gene were studied by cell culture. Rb-MBG loaded with antibacterial agents enoxacin (ENX), coliforms and Staphylococcus aureus were cultured together to study the antibacterial effects. The results indicate that the samples have a 350-550 μm large pore structure and 4.5-5.5 nm mesoporous size. Adding Rb can increase the activity of ALP, the secretion of VEGF and COLI, and the expression of HIF-1α of hBMSCs. Rb containing MBG is likely to enhance the proliferation and differentiation of hBMSCs through the influence of Wnt/ß-catenin signal path. Rb-MBG scaffold can load effectively and release Rb ions and ENX continuously to damage the bacterial cell membrane with the synergistic effect, and therefore achieve antibacterial results. In conclusion, adding Rb to MBG supports angiogenesis and osteogenesis of hBMSCs, as well as antibacterial activity.
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http://dx.doi.org/10.1016/j.msec.2019.110155DOI Listing
December 2019

miR-466 and NUS1 Regulate the AKT/Nuclear Factor kappa B (NFκB) Signaling Pathway in Intrauterine Adhesions in a Rat Model.

Med Sci Monit 2019 Jun 2;25:4094-4103. Epub 2019 Jun 2.

Department of Obstetrics and Gynecology, Affiliated Qianfoshan Hospital of Shandong University, Jinan, Shandong, China (mainland).

BACKGROUND Intrauterine adhesions (IUAs) are one of the most common reproductive system diseases in women worldwide. Emerging evidence has demonstrated that the upregulation or downregulation of genes plays an important role in IUAs. The aim of this study was to evaluate the role of NUS1 in IUAs in a rat model. MATERIAL AND METHODS The expression of miR-466 in intrauterine adhesions tissues was detected by using RT-qPCR assay. RT-qPCR, IHC, and Western blot were used to investigate mRNA and proteins expression, respectively, of NUS1. MTT and colony-formation assays were used to evaluate cell growth. Transwell assays were used to detect cell migration and invasion. To investigate miR-466 and NUS1 functions in vivo, we established a rat model. The level of epithelial-to-mesenchymal transition (EMT)-related markers was analyzed by Western blot assay. RESULTS NUS1 was upregulated in IUAs tissues, and the high expression level of NUS1 was positively correlated with the severity of IUAs. NUS1 promoted cell proliferation in vitro. NUS1 overexpression on cell migration and invasion promoted the EMT process in vitro and in vivo. NUS1 acted as a target of miR-466 and played the stimulative role by regulating AKT/NFkappaB pathway. CONCLUSIONS Our data suggest that miR-466 and NUS1 regulate proliferation and the EMT process through the AKT/NFkappaB pathway in IUAs in a rat model.
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http://dx.doi.org/10.12659/MSM.914202DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6561142PMC
June 2019

Buoyant hydrous mantle plume from the mantle transition zone.

Sci Rep 2019 Apr 25;9(1):6549. Epub 2019 Apr 25.

Graduate School of Science, Hokkaido University, Sapporo, Japan.

Magmatism at some intraplate volcanoes and large igneous provinces (LIPs) in continental areas may originate from hydrous mantle upwelling (i.e. a plume) from the mantle transition zone (MTZ) at 410-660 km depths in the Earth's deep interior. However, the ultimate origin of the magmatism, i.e. why mantle plumes could have been generated at the MTZ, remains unclear. Here, we study the buoyancy of a plume by investigating basalts from the Changbaishan volcano, beneath which a mantle plume from the hydrous MTZ is observed via seismology. Based on carefully determined water contents of the basalts, the potential temperature of the source mantle is estimated to be 1310-1400 °C, which is within the range of the normal upper mantle temperature. This observation suggests that the mantle plume did not have a significant excess heat, and that the plume upwelled because of buoyancy resulting from water supplied from the Pacific slab in the MTZ. Such a hydrous mantle plume can account for the formation of extremely hydrous LIP magmatism. The water was originally sourced from a stagnant slab and stored in the MTZ, and then upwelled irrespective of the presence or absence of a deep thermal plume.
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http://dx.doi.org/10.1038/s41598-019-43103-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6484022PMC
April 2019

Tomography of the 2016 Kumamoto earthquake area and the Beppu-Shimabara graben.

Sci Rep 2018 Oct 19;8(1):15488. Epub 2018 Oct 19.

Department of Geophysics, Tohoku University, Sendai, 980-8578, Japan.

Detailed three-dimensional images of P and S wave velocity and Poisson's ratio (σ) of the crust and upper mantle beneath Kyushu in SW Japan are determined, with a focus on the source area of the 2016 Kumamoto earthquake (M 7.3) that occurred in the Beppu-Shimabara graben (BSG) where four active volcanoes and many active faults exist. The 2016 Kumamoto earthquake took place in a high-velocity and low-σ zone in the upper crust, which is surrounded and underlain by low-velocity and high-σ anomalies in the upper mantle. This result suggests that, in and around the source zone of the 2016 Kumamoto earthquake, strong structural heterogeneities relating to active volcanoes and magmatic fluids exist, which may affect the seismogenesis. Along the BSG, low-velocity and high-σ anomalies do not exist everywhere in the upper mantle but mainly beneath the active volcanoes, suggesting that hot mantle upwelling is not the only cause of the graben. The BSG was most likely formed by joint effects of northward extension of the Okinawa Trough, westward extension of the Median Tectonic Line, and hot upwelling flow in the mantle wedge beneath the active volcanoes.
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http://dx.doi.org/10.1038/s41598-018-33805-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6195613PMC
October 2018

Zero-dimensional, one-dimensional, two-dimensional and three-dimensional biomaterials for cell fate regulation.

Adv Drug Deliv Rev 2018 07 30;132:33-56. Epub 2018 Jun 30.

Department of Biomedical Engineering, College of Biology, Hunan University, Changsha 410082, China; Shenzhen Research Institute of Hunan University, Nanshan Hi-new Technology and Industry Park, Shenzhen 518057, China. Electronic address:

The interaction of biological cells with artificial biomaterials is one of the most important issues in tissue engineering and regenerative medicine. The interaction is strongly governed by physical and chemical properties of the materials and displayed with differentiated cellular behaviors, including cell self-renewal, differentiation, reprogramming, dedifferentiation, or transdifferentiation as a result. A number of engineered biomaterials with micro- or nano-structures have been developed to mimic structural components of cell niche and specific function of extra cellular matrix (ECM) over past two decades. In this review article, we briefly introduce the fabrication of biomaterials and their classification into zero-dimensional (0D), one-dimensional (1D), two-dimensional (2D) and three-dimensional (3D) ones. More importantly, the influence of different biomaterials on inducing cell self-renewal, differentiation, reprogramming, dedifferentiation, and transdifferentiation was discussed based on the progress at 0D, 1D, 2D and 3D levels, following which the current research limitations and research perspectives were provided.
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http://dx.doi.org/10.1016/j.addr.2018.06.020DOI Listing
July 2018

Upper and lower plate controls on the great 2011 Tohoku-oki earthquake.

Authors:
Xin Liu Dapeng Zhao

Sci Adv 2018 06 20;4(6):eaat4396. Epub 2018 Jun 20.

Department of Geophysics, Tohoku University, Sendai 980-8578, Japan.

The great 2011 Tohoku-oki earthquake [moment magnitude () 9.0)] is the best-documented megathrust earthquake in the world, but its causal mechanism is still in controversy because of the poor state of knowledge on the nature of the megathrust zone. We constrain the structure of the Tohoku forearc using seismic tomography, residual topography, and gravity data, which reveal a close relationship between structural heterogeneities in and around the megathrust zone and rupture processes of the 2011 Tohoku-oki earthquake. Its mainshock nucleated in an area with high seismic velocity, low seismic attenuation, and strong seismic coupling, probably indicating a large asperity (or a cluster of asperities) in the megathrust zone. Strong coseismic high-frequency radiations also occurred in high-velocity patches, whereas large afterslips took plate in low-velocity areas, differences that may reflect changes in fault friction and lithological variations. These structural heterogeneities in and around the Tohoku megathrust originate from both the overriding and subducting plates, which controlled the nucleation and rupture processes of the 2011 Tohoku-oki earthquake.
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http://dx.doi.org/10.1126/sciadv.aat4396DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6010320PMC
June 2018

Inhibition of HeLa cell growth by doxorubicin-loaded and tuftsin-conjugated arginate-PEG microparticles.

Bioact Mater 2018 Mar 6;3(1):48-54. Epub 2017 May 6.

Institute of Bionanotechnology and Tissue Engineering, College of Life Sciences, Hunan University, Changsha, 410082, China.

In order to improve the release pattern of chemotherapy drug and reduce the possibility of drug resistance, poly(ethylene glycol amine) (PEG)-modified alginate microparticles (ALG-PEG MPs) were developed then two different mechanisms were employed to load doxorubicin (Dox): 1) forming Dox/ALG-PEG complex by electrostatic attractions between unsaturated functional groups in Dox and ALG-PEG; 2) forming Dox-ALG-PEG complex through EDC-reaction between the amino and carboxyl groups in Dox and ALG, respectively. Additionally, tuftsin (TFT), a natural immunomodulation peptide, was conjugated to MPs in order to enhance the efficiency of cellular uptake. It was found that the Dox-ALG-PEG-TFT MPs exhibited a significantly slower release of Dox than Dox/ALG-PEG-TFT MPs in neutral medium, suggesting the role of covalent bonding in prolonging Dox retention. Besides, the release of Dox from these MPs was pH-sensitive, and the release rate was observably increased at pH 6.5 compared to the case at pH 7.4. Compared with Dox/ALG-PEG MPs and Dox-ALG-PEG MPs, their counterparts further conjugated with TFT more efficiently inhibited the growth of HeLa cells over a period of 48 h, implying the effectiveness of TFT in enhancing cellular uptake of MPs. Over a period of 48 h, Dox-ALG-PEG-TFT MPs inhibited the growth of HeLa cells less efficiently than Dox/ALG-PEG-TFT MPs but the difference was not significant ( > 0.05). In consideration of the prolonged and sustained release of Dox, Dox-ALG-PEG-TFT MPs possess the advantages for long-term treatment.
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http://dx.doi.org/10.1016/j.bioactmat.2017.04.007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5935659PMC
March 2018

Inhibitory effect of the mitogen activated protein kinase specific inhibitor PD98059 on Mtb-Ag-activated γδΤ cells.

Int J Clin Exp Pathol 2017 1;10(9):9644-9648. Epub 2017 Sep 1.

Department of Breast Surgery, The Affiliated Hospital of Taishan Medical University Tai'an, Shandong Province, China.

Objective: This study aimed to observe the inhibitory effects of mitogen activated protein kinase (MAPK/ERK) specific inhibitor PD98059 on Mtb-Ag activated γδΤ cells and to investigate the role of MAPK in MAPK/ERK pathway of γδΤ cells activated by Mtb-Ag.

Methods: Healthy human peripheral blood mononuclear cell (PBMC) was isolated from the peripheral blood, and then stimulated by phorbol esters (PMA), ionomycin (IM) and Mtb-Ag. PBMC of the experimental group was pretreated by PD98059 of 0, 1, 10 or 100 μmol/L while the control group was given no pretreatment. Flow cytometry was applied to detect the expression of CD69 in γδΤ cells at 0 h, 6 h, 12 h, 24 h, 48 h and 72 h after treatment respectively. The influence of PD98059 on the amplification of γδΤ cells was also detected.

Results: After stimulated by PMA and IM for 6 hours, the expression of CD69 in γδΤ reached the peak of (99.3±1.09)%, while in the Mtb-Ag stimulation group, it reached the highest level of 75.2% at 24 hours. The level of CD69 in two group was significant difference between the two groups at each time respectively (p<0.05). Pretreated with PD98059 of 0, 1, 10, 100 μmol/L, the level of CD 69 expression in Mtb-Ag stimulated γδΤ was 79±0.8%, 75±0.7%, 54±0.5% and 17±0.2% respectively; In PMA+IM stimulated group, CD69 expression were all more than 98% in different concentration of PD98059. After treated with PD98059 of 0, 1, 10, 100 μmol/L, total cell number increased from 1.5×10 to (10.3±2.5)×10, (9.5±2.1)×10, (5.8±1.8)×10 and (2.1±0.5)×10 respectively. Number of γδΤ cells reached to (6.2±0.9)×10, (5.02±0.8)×10, (2.05±0.5)×10 and (0.41±0.1)×10 respectively.

Conclusion: γδΤ cells were specifically activated by Mtb-Ag, and the activation was depended on MAPK/ERK pathway. The activation effects of Mtb-Ag on γδΤ cells can be obviously inhibited by PD98059.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6965926PMC
September 2017

Seismic anisotropy evidence for dehydration embrittlement triggering intermediate-depth earthquakes.

Sci Rep 2017 06 1;7(1):2613. Epub 2017 Jun 1.

Key Laboratory of Earth and Planetary Physics, Institute of Geology and Geophysics, Chinese Academy of Sciences, Beijing, 100029, China.

It has been proposed that dehydration embrittlement of hydrous materials can trigger intermediate-depth earthquakes and form a double seismic zone in a subducting slab. Seismic anisotropy may provide a possible insight into intermediate-depth intraslab seismicity, because anisotropic properties of minerals change with varying water distribution, temperature and pressure. Here we present a high-resolution model of P-wave radial anisotropy tomography of the Japan subduction zone down to ~400 km depth, which is obtained using a large number of arrival-time data of local earthquakes and teleseismic events. Our results reveal a close correlation between the pattern of intermediate-depth seismicity and anisotropic structures. The seismicity occurs in portions of the Pacific and Philippine Sea slabs where positive radial anisotropy (i.e., horizontal velocity being faster than vertical one) dominates due to dehydration, whereas the inferred anhydrous parts of the slabs are found to be aseismic where negative radial anisotropy (i.e., vertical velocity being faster than horizontal one) dominates. Our anisotropic results suggest that intermediate-depth earthquakes in Japan could be triggered by dehydration embrittlement of hydrous minerals in the subducting slabs.
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http://dx.doi.org/10.1038/s41598-017-02563-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5453959PMC
June 2017

Tomography of the subducting Pacific slab and the 2015 Bonin deepest earthquake (Mw 7.9).

Sci Rep 2017 03 15;7:44487. Epub 2017 Mar 15.

Department of Geophysics, Tohoku University, Sendai 980-8578, Japan.

On 30 May 2015 an isolated deep earthquake (~670 km, Mw 7.9) occurred to the west of the Bonin Islands. To clarify its causal mechanism and its relationship to the subducting Pacific slab, we determined a detailed P-wave tomography of the deep earthquake source zone using a large number of arrival-time data. Our results show that this large deep event occurred within the subducting Pacific slab which is penetrating into the lower mantle. In the Izu-Bonin region, the Pacific slab is split at ~28° north latitude, i.e., slightly north of the 2015 deep event hypocenter. In the north the slab becomes stagnant in the mantle transition zone, whereas in the south the slab is directly penetrating into the lower mantle. This deep earthquake was caused by joint effects of several factors, including the Pacific slab's fast deep subduction, slab tearing, slab thermal variation, stress changes and phase transformations in the slab, and complex interactions between the slab and the ambient mantle.
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http://dx.doi.org/10.1038/srep44487DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5353660PMC
March 2017

Estimation of stature by using the dimensions of the right hand and right foot in Han Chinese adults.

Sci China Life Sci 2017 Jan 21;60(1):81-90. Epub 2016 Dec 21.

Institute of Biological Anthropology in Liaoning Medical University, Jinzhou, 121000, China.

The Han Chinese people are the main ethnic group in China and the largest ethnic group in the world. The dimensions of the hands and feet have been successfully used for the estimation of stature. A total of 26,927 healthy adult subjects, comprising 13,221 men and 13,706 women, were recruited. The survey samples were chosen through random cluster sampling. The mean values were significantly higher in men than those in women for all measurements (P<0.001). All the measurements showed a statistically significant correlation with stature (P<0.01). The combination of the right hand length and the right foot length was the best predictor of stature because it had the lowest standard error of estimate. The use of multiple regression equations yielded better results than did the use of linear regression equations. The accuracy of stature prediction ranged from ±4.81 to ±6.39 cm. The present study was of great importance with regards to improving the physical anthropology database of ethnic groups in China.
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http://dx.doi.org/10.1007/s11427-016-0051-8DOI Listing
January 2017

Groundwater helium anomaly reflects strain change during the 2016 Kumamoto earthquake in Southwest Japan.

Sci Rep 2016 11 29;6:37939. Epub 2016 Nov 29.

Department of Geophysics, Tohoku University, Sendai, Miyagi 980-8578, Japan.

Geochemical monitoring of groundwater and soil gas emission pointed out precursor and/or coseismic anomalies of noble gases associated with earthquakes, but there was lack of plausible physico-chemical basis. A laboratory experiment of rock fracturing and noble gas emission was conducted, but there is no quantitative connection between the laboratory results and observation in field. We report here deep groundwater helium anomalies related to the 2016 Kumamoto earthquake, which is an inland crustal earthquake with a strike-slip fault and a shallow hypocenter (10 km depth) close to highly populated areas in Southwest Japan. The observed helium isotope changes, soon after the earthquake, are quantitatively coupled with volumetric strain changes estimated from a fault model, which can be explained by experimental studies of helium degassing during compressional loading of rock samples. Groundwater helium is considered as an effective strain gauge. This suggests the first quantitative linkage between geochemical and seismological observations and may open the possibility to develop a new monitoring system to detect a possible strain change prior to a hazardous earthquake in regions where conventional borehole strain meter is not available.
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http://dx.doi.org/10.1038/srep37939DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5126567PMC
November 2016

Preliminary study on hand preference in captive northern white-cheeked gibbons (Nomascus leucogenys).

Primates 2017 Jan 14;58(1):75-82. Epub 2016 Sep 14.

Ministry of Education, Key Laboratory of Biodiversity Science and Ecological Engineering, College of Life Sciences, Beijing Normal University, Beijing, People's Republic of China.

The postural origin hypothesis and the task complexity hypothesis propose that hand preference in non-human primates evolved in association with body posture and task complexity, respectively. The results of previous studies testing these two hypotheses, however, vary greatly with the different primate species and methods used. To investigate the effect of body posture and task complexity on hand preference, we recorded bouts of hand usage in nine captive northern white-cheeked gibbons (Nomascus leucogenys) housed at Beijing Zoo as they reached for food items in a ground-reaching task, a box task, and a tube task. The results showed that four to seven of the nine gibbons displayed a hand preference at the individual level in different tasks, and that hand preference in individuals was task-specific; there was no group-level hand preference in any task. The box task seemed to elicit a greater strength of hand preference than the ground-reaching task at the individual level. Although the small sample size rules out drawing any strong conclusions concerning hand preference at the group level, our results suggest that the suspensory reaching posture might increase the expression of hand preference at the individual level. This study provides preliminary information on hand preference in captive northern white-cheeked gibbons, and will be helpful for future studies addressing the origin and evolution of hand preference in small apes.
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http://dx.doi.org/10.1007/s10329-016-0573-8DOI Listing
January 2017

Hand preference during bimanual coordinated task in northern pig-tailed macaques .

Curr Zool 2016 Aug 27;62(4):385-391. Epub 2016 Jul 27.

Tianjin Key Laboratory of Animal and Plant Resistance, College of Life Sciences, Tianjin Normal University, Tianjin, 300387.

In humans, handedness is one defining characteristic regardless of cultures and ethnicity. Population-level right handedness is considered to be related with the evolution of left hemisphere for manual control and language. In order to further understand evolutionary origins of human cerebral lateralization and its behavioral adaptation, standardized measures on hand preference are required to make reliable comparison in nonhuman primate species. In this study, we present the first evidence on hand preference during bimanual coordinated tasks in northern pig-tailed macaques . The classical TUBE task was applied to examine hand preference among nine individuals from Tianjin Zoo of China. We recorded and made analysis on both frequency and bout data on manual laterality. The results consistently show that subjects displayed strong individual hand preferences, whereas no significant group-level handedness was found. There were no sex and age significant differences on both direction and strength of hand preference. The preferred to use the index finger to extract the baited food inside the tube. Our findings fill the knowledge gap on primate handedness, and efficiently affirm the robustness of the TUBE task as one efficient measure of hand preference in primates.
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http://dx.doi.org/10.1093/cz/zow064DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5804279PMC
August 2016

Depth variations of P-wave azimuthal anisotropy beneath Mainland China.

Sci Rep 2016 07 19;6:29614. Epub 2016 Jul 19.

Key Laboratory of Computational Geodynamics, Chinese Academy of Sciences, Beijing 100049, China.

A high-resolution model of P-wave anisotropic tomography beneath Mainland China and surrounding regions is determined using a large number of arrival-time data recorded by the China seismic network, the International Seismological Centre (ISC) and temporary seismic arrays deployed on the Tibetan Plateau. Our results provide important new insights into the subducted Indian plate and mantle dynamics in East Asia. Our tomographic images show that the northern limit of the subducting Indian plate has reached the Jinsha River suture in eastern Tibet. A striking variation of P-wave azimuthal anisotropy is revealed in the Indian lithosphere: the fast velocity direction (FVD) is NE-SW beneath the Indian continent, whereas the FVD is arc parallel beneath the Himalaya and Tibetan Plateau, which may reflect re-orientation of minerals due to lithospheric extension, in response to the India-Eurasia collision. There are multiple anisotropic layers with variable FVDs in some parts of the Tibetan Plateau, which may be the cause of the dominant null splitting measurements in these regions. A circular pattern of FVDs is revealed around the Philippine Sea slab beneath SE China, which reflects asthenospheric strain caused by toroidal mantle flow around the edge of the subducting slab.
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http://dx.doi.org/10.1038/srep29614DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4949423PMC
July 2016

Effect of target animacy on hand preference in Sichuan snub-nosed monkeys (Rhinopithecus roxellana).

Anim Cogn 2016 09 6;19(5):977-85. Epub 2016 Jun 6.

College of Life Sciences, Northwest University, Xi'an, China.

Twenty-eight captive Sichuan snub-nosed monkeys (Rhinopithecus roxellana) were involved in the current study. Many individuals showed handedness, with a modest tendency toward left-hand use especially for animate targets, although no group-level handedness was found. There was no significant gender difference in the direction and strength of hand preference for both targets. Females showed a significantly higher overall rate of actions toward animate targets than inanimate targets for both hands, whereas males displayed almost the reversed pattern. There were no significant interactions between lateral hand use and target animacy for either males or females. Most individuals showed rightward or leftward laterality shift trends between inanimate and animate targets. These findings to some extent support the existence of a potential trend concerning a categorical neural distinction between targets demanding functional manipulation (inanimate objects) and those demanding social manipulation (animate objects), even though specialized hand preference based on target animacy has not been fully established in this arboreal Old World monkey species.
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http://dx.doi.org/10.1007/s10071-016-1002-5DOI Listing
September 2016
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