Publications by authors named "Danni Liu"

75 Publications

Effects of Ultrashort Wave Therapy on Inflammation and Macrophage Polarization after Acute Lung Injury in Rats.

Bioelectromagnetics 2021 Jun 15. Epub 2021 Jun 15.

Rehabilitation Medicine Center, The First Affiliated Hospital of University of South China, Hengyang, People's Republic of China.

Acute lung injury (ALI) features dysregulated pulmonary inflammation. Ultrashort waves (USWs) exert anti-inflammatory effects but no studies have evaluated their activity in ALI. Herein, we used an in vivo lipopolysaccharide (LPS)-induced ALI model to investigate whether the anti-inflammatory activity of USWs is mediated by altering the polarization of M1 to M2 macrophages. Twenty-four male Sprague-Dawley rats were randomly divided into control, untreated ALI, and ALI treated with USW groups (n = 8 in each group). ALI was induced by intratracheal LPS instillation. Rats in the USW group were treated for 15 min at 0, 4, and 8 h after a single LPS intratracheal instillation. Histopathologic examination, wet/dry lung weight ratio, enzyme-linked immunosorbent assay, quantitative real-time polymerase chain reaction, and western blot analyses were performed to evaluate the degree of lung injury and to determine macrophage phenotypes. Histopathologic examination disclosed attenuation of ALI, with reduced alveolar hemorrhage and neutrophilic infiltration in the USW group. Serum levels of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) were significantly decreased after USW therapy. Moreover, the messenger RNA (mRNA) expressions of TNF-α and IL-1β were significantly decreased in the USW group, whereas the mRNA expression of Arginase 1 (Arg1) and the protein expression of mannose receptor significantly increased in comparison with the untreated ALI group. We conclude that USW therapy may attenuate inflammation in LPS-induced ALI through the modulation of macrophage polarization. © 2021 Bioelectromagnetics Society.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/bem.22353DOI Listing
June 2021

Genetic architecture of root and shoot ionomes in rice (Oryza sativa L.).

Theor Appl Genet 2021 Aug 20;134(8):2613-2637. Epub 2021 May 20.

Plant Breeding and Genetics Section, School of Integrative Plant Science, Cornell University, Ithaca, NY, 14853-1901, USA.

Key Message: Association analysis for ionomic concentrations of 20 elements identified independent genetic factors underlying the root and shoot ionomes of rice, providing a platform for selecting and dissecting causal genetic variants. Understanding the genetic basis of mineral nutrient acquisition is key to fully describing how terrestrial organisms interact with the non-living environment. Rice (Oryza sativa L.) serves both as a model organism for genetic studies and as an important component of the global food system. Studies in rice ionomics have primarily focused on above ground tissues evaluated from field-grown plants. Here, we describe a comprehensive study of the genetic basis of the rice ionome in both roots and shoots of 6-week-old rice plants for 20 elements using a controlled hydroponics growth system. Building on the wealth of publicly available rice genomic resources, including a panel of 373 diverse rice lines, 4.8 M genome-wide single-nucleotide polymorphisms, single- and multi-marker analysis pipelines, an extensive tome of 321 candidate genes and legacy QTLs from across 15 years of rice genetics literature, we used genome-wide association analysis and biparental QTL analysis to identify 114 genomic regions associated with ionomic variation. The genetic basis for root and shoot ionomes was highly distinct; 78 loci were associated with roots and 36 loci with shoots, with no overlapping genomic regions for the same element across tissues. We further describe the distribution of phenotypic variation across haplotypes and identify candidate genes within highly significant regions associated with sulfur, manganese, cadmium, and molybdenum. Our analysis provides critical insight into the genetic basis of natural phenotypic variation for both root and shoot ionomes in rice and provides a comprehensive resource for dissecting and testing causal genetic variants.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00122-021-03848-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8277617PMC
August 2021

The mutational landscape and prognostic indicators of pseudomyxoma peritonei originating from the ovary.

Int J Cancer 2021 04 11;148(8):2036-2047. Epub 2021 Jan 11.

Department of Myxoma, Aerospace Center Hospital, Beijing, China.

Pseudomyxoma peritonei (PMP) is a rare disorder with unique pathological and genetic changes. Although several studies have reported the clinical features and mutational changes of PMP that originates from the appendix, few studies on PMP originating from the ovary have been reported due to its extreme rarity. In order to characterize the somatic mutational landscape and to investigate the prognosis predicting factors of ovary-originating PMP, we examined 830 cases of PMP and identified 16 patients with PMP that originated from the ovary. Whole-exome sequencing (WES) was performed on 12 cases using formalin-fixed, paraffin-embedded (FFPE) tissue samples. We found that 25% (3/12) of the patients carried mutations in cancer driver genes, including TP53, ATM and SETD2, and 16.7% (2/12) of the patients carried mutations in cancer driver genes, including ATRX, EP300, FGFR2, KRAS, NOCR1 and RB1. The MUC16 (58.33%), BSN (41.67%), PCNT (41.67%), PPP2R5A (41.67%), PRSS36 (41.67%), PTPRK (41.67%) and SBF1 (41.67%) genes presented the highest mutational frequencies. The PI3K-Akt signaling pathway, human papillomavirus infection pathway, cell skeleton, cell adhesion, and extracellular matrix and membrane proteins were the major pathways or functions that were affected. Patients were followed up to 174 months (median: 48.26 months). The 5-year OS rate for all patients was 71.2% and the median OS was not reached. PTPRK mutations, presurgical CA199 level, completeness of cytoreduction (CCR) and peritoneal cancer index (PCI) were identified as potential predictive factors for patient survival. In conclusion, the mutational landscape for ovary-originating PMP was revealed and exhibited unique features distinct from appendix-originating PMP. PTPRK, CA199, CCR and PCI may predict patient survival.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/ijc.33460DOI Listing
April 2021

Silicon monophosphides with controlled size and crystallinity for enhanced lithium anodic performance.

Nanoscale 2021 Jan 16;13(1):51-58. Epub 2020 Dec 16.

Shenzhen Engineering Center for the Fabrication of Two-Dimensional Atomic Crystals, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, P. R. China.

New electrode materials are crucial to high-performance lithium-ion batteries (LIBs). Silicon monophosphides (SiPs), composed of silicon and phosphorus, have a very high theoretical capacity (3060 mA h g), which is more than 8 times that of graphite (372 mA h g). The two-dimensional structure of SiPs also benefits ion transportation and diffusion. In this work, the chemical vapor transport (CVT) method is employed to synthesize SiPs for LIB anodes, and the lithium storage capacity co-affected by size and crystallinity is investigated using controllably synthesized thin belts and bulk crystals. The SiPs prepared by the high-temperature iodine-assisted CVT method have a belt-like morphology about 72 nm thick. After 200 cycles, the stable capacity is about 615 mA h g at 100 mA g, and a reversible capacity of ∼320 mA h g is achieved at a high current density of 5.0 A g. In contrast, the micrometer-thick bulk SiP crystals cannot provide efficient lithium ion extraction. Moreover, the smaller and thinner SiPs obtained at a lower temperature show abnormally high mass transport resistance and low lithium ion diffusivity. These results demonstrate that SiPs are promising LIB anode materials, and the size and crystallinity are closely related to the anodic performance. This new knowledge is valuable for the development of high-performance LIBs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d0nr07386eDOI Listing
January 2021

Dietary taurine modulates hepatic oxidative status, ER stress and inflammation in juvenile turbot (Scophthalmus maximus L.) fed high carbohydrate diets.

Fish Shellfish Immunol 2021 Feb 5;109:1-11. Epub 2020 Dec 5.

The Key Laboratory of Aquaculture Nutrition and Feeds, Ministry of Agriculture and Rural Affairs, The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, 266003, PR China.

This study was conducted to explore the beneficial role of taurine against chronic high carbohydrate diet-induced oxidative stress, endoplasmic reticulum (ER) stress and inflammation, and to understand the underlying molecular mechanisms in turbot. Two 10-week feeding trials were simultaneously conducted. For the one, six experimental diets with graded levels of taurine supplementation (0, 0.4%, 0.8%, 1.2%, 1.6% and, 2.0%, respectively) and 15% of carbohydrate were used. For the other one, three graded levels of dietary taurine supplementation (0.4%, 1.2% and 2.0%, respectively) with 21% of carbohydrate were used. The results showed that higher expression level of inflammation cytokines and ER stress related genes were detected in higher dietary carbohydrate group. In both feeding trials, 1.2% of dietary taurine supplementation improved anti-oxidative status by decreasing the content of malondialdehyde, increasing the catalase activity and total anti-oxidative capacities. In feeding trial 1, appropriate taurine supplementation lowered contents of tumour necrosis factor-a, interleukin-6, aspartate aminotransferase and alkaline phosphatase in plasma, and decreased the expressions of pro-inflammatory cytokines, such as interleukin-8 (il-8) and interferon-γ (ifn-γ). Furthermore, dietary taurine reduced ER stress by decreasing the mRNA levels of activating transcription factor 6, protein kinase R-like endoplasmic reticulum kinase and G protein-coupled receptor 78. The optimal dietary taurine content was estimated as 1.40% based on the analysis of specific growth rate. In feeding trial 2, dietary taurine supplementation attenuated liver inflammation partly referring to significantly down-regulated mRNA levels of nuclear transcription factor-κB p65, ifn-γ, interleukin1β and up-regulate the transcript of ribosomal protein S6 kinase 1. Dietary taurine supplementation in feeding trial 2 significantly increased the Nrf2-related factor 2 protein level and decreased the NFκB p65 protein level only at 21% of dietary carbohydrate level. Taurine can alleviate the oxidative damage and inflammation caused by 21% of dietary carbohydrate to a certain degree. Overall, the present study confirmed that dietary taurine supplementation improved growth performance and anti-oxidative response, and reduced liver inflammatory and ER stress processes induced by high dietary carbohydrate in turbot.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.fsi.2020.11.029DOI Listing
February 2021

Apelin‑36 protects against lipopolysaccharide‑induced acute lung injury by inhibiting the ASK1/MAPK signaling pathway.

Mol Med Rep 2021 01 12;23(1). Epub 2020 Nov 12.

Department of Cardiology, People's Hospital of Ningxia Hui Autonomous Region (Ningxia Medical University Affiliated Autonomous Region People's Hospital), Yinchuan, Ningxia 750002, P.R. China.

Apelin‑36 is able to mediate a range of effects on various diseases, and is upregulated in lipopolysaccharide (LPS)‑induced acute lung injury (ALI). However, to the best of our knowledge, whether apelin‑36 is able to regulate LPS‑induced ALI has yet to be investigated. The present study aimed to investigate the role of apelin‑36 in LPS‑induced ALI, and the putative underlying mechanisms. Rats were assigned to one of four treatment groups: The Control group, apelin‑36 group, LPS group and LPS + apelin‑36 group. At 4 h after intratracheal instillation of LPS (5 mg/kg), rats were intraperitoneally treated with 10 nmol/kg apelin‑36. Subsequently, pathological manifestations and the extent of inflammation and apoptosis of the lung tissues were assessed. Untransfected and apoptosis signal‑regulating kinase 1 (ASK1)‑overexpressing Beas‑2B cells were treated with LPS in the absence or presence of apelin‑36, and subsequently the levels of inflammation and apoptosis were assessed. The results obtained showed that the level of apelin‑36 was increased in the bronchoalveolar lavage fluid (BALF) of LPS‑treated rats. Co‑treatment with apelin‑36 alleviated LPS‑induced lung injury and pulmonary edema, reduced the levels of pro‑inflammatory cytokines, including interleukin‑6, monocyte chemoattractant protein‑1 and tumor necrosis factor‑α, in BALF, and inhibited apoptosis in the lung tissues. The presence of apelin‑36 also blocked the activation of LPS‑induced ASK1, p38, c‑Jun N‑terminal kinase and extracellular signal‑regulated kinase in lung tissues. In vitro studies performed with Beas‑2B cells showed that the addition of apelin‑36 led to an increase in the cell viability of LPS‑induced Beas‑2B cells in a concentration‑dependent manner. Additionally, co‑treatment with 1 µM apelin‑36 prevented LPS‑induced inflammation and apoptosis. However, overexpression of ASK1 significantly reversed the inhibitory effects of apelin‑36 on LPS‑induced inflammation and apoptosis. Taken together, the results of the present study demonstrated that apelin‑36 was able to protect against LPS‑induced lung injury both in vivo and in vitro, and these actions may be dependent on inhibition of the ASK1/mitogen‑activated protein kinase signaling pathway.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3892/mmr.2020.11644DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7673347PMC
January 2021

Metastatic Low-Grade Sarcoma with CARS-ALK Fusion Dramatically Responded to Multiple ALK Tyrosine Kinase Inhibitors: A Case Report with Comprehensive Genomic Analysis.

Oncologist 2021 04 16;26(4):e524-e529. Epub 2020 Oct 16.

Department of Medical Oncology, Changzheng Hospital, Shanghai, People's Republic of China.

This article reports a case of advanced metastatic low-grade sarcoma. The patient was diagnosed with an inoperable large (14 × 12 cm) lesion on his neck in September 2015 and underwent two ineffective chemotherapies in the following 4 months. Interestingly, although several pathologists could not agree on the histopathological diagnosis, the precise molecular pathological diagnosis was obtained using next-generation sequencing (NGS) and finally brought excellent therapeutic effects. The patient was detected to have CARS-ALK fusion by NGS and then was successfully treated with crizotinib orally. He received surgical resection of primary and metastatic lesions after tumor shrinkage. The combined treatment brought a durable response for 40 months. Although the tumor recurred in July 2019, the patient has been responding well to the second-line ALK tyrosine kinase inhibitor alectinib to date. We performed whole genome sequencing on the patient's primary, metastatic, and recurrent tumors and did comprehensive genomic analysis. Furthermore, our analysis results revealed that a whole genome duplication event might have happened during tumorigenesis of this case. KEY POINTS: To our best knowledge, this is the first report of a very successful treatment with first- and second-line ALK tyrosine kinase inhibitors for CARS-ALK fusion-positive metastatic low-grade sarcoma. Molecular pathological result can guide precision treatment for sarcoma, even when the exact histopathology cannot be obtained. Multiple samples from this patient were analyzed using whole genome sequencing. Results provided detailed genomic characteristics and showed tumor evolution of this low-grade sarcoma case. A whole genome duplication event might have happened during tumorigenesis of this low-grade sarcoma case.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/onco.13543DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8018318PMC
April 2021

Next-Generation Sequencing Analysis of ctDNA for the Detection of Glioma and Metastatic Brain Tumors in Adults.

Front Neurol 2020 21;11:544. Epub 2020 Aug 21.

Department of Neurosurgery, China-Japan Friendship Hospital, Beijing, China.

The next-generation sequencing technologies and their related assessments of circulating tumor DNA in both glioma and metastatic brain tumors remain largely limited. Based largely on a protocol approved by the institutional review board at Peking University International Hospital, the current retrospective, single-center study was conducted. Genomic DNA was extracted from blood samples or tumor tissues. With the application of NextSeq 500 instrument (Illumina), Sequencing was performed with an average coverage of 550-fold. Paired-end sequencing was employed utilized with an attempt to achieve improved sensitivity of duplicate detection and therefore to increase the detection reliability of possible fusions. A total of 28 patients (21 men and 7 women) with brain tumors in the present study were involved in the study. The patients enrolled were assigned into two groups, including glioma group ( = 21) and metastatic brain tumor group ( = 7). The mean age of metastatic brain tumor group (59.86 ± 8.85 y), (43.65 ± 13.05 y) reported significantly higher results in comparison to that of glioma group (45.3 ± 12.3 years) ( < 0.05). The mutant genes in metastatic brain tumor group included and ; however, there were no glioma-related mutant genes including , IDH2, , and BRAF et al. Interesteringly, only two patient (28.3%) was detected blood ctDNA in metastatic brain tumor group; In contrast, blood ctDNA was found in ten glioma patients (47.6%) including 1p/19q, . The characterizations of mutations in the glioma included mutation (p.R132H) and IDH2 mutation (p.R172K). The mutation rate of IDH in tumor tissues was 37.06 ± 8.32%, which was significantly higher than blood samples ( < 0.05). The present study demonstrated that the mutant genes among glioma and metastatic brain tumors are shown to be different. Moreover, the ctDNAs in the metastatic brain tumors included and , and glioma-related ctDNAs included 1p/19q and followed by frequencies of . These ctDNAs might be biomarkers and therapeutic responders in brain tumor.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fneur.2020.00544DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7473301PMC
August 2020

Intraindividual reaction time variability, respiratory sinus arrhythmia, and children's externalizing problems.

Int J Psychophysiol 2020 11 1;157:1-10. Epub 2020 Sep 1.

School of Psychology, Shaanxi Normal University, Shaanxi Provincial Key Research Center of Child Mental and Behavioral Health, Xi'an, China. Electronic address:

Intraindividual reaction time variability (IIRTV) is a unique cognitive function index that is independent of accuracy and has been found to be related to children's externalizing problems. Respiratory sinus arrhythmia (RSA) reactivity to challenge tasks was also found to be a potential influence factor of children's externalizing problems. The present study examined whether IIRTV interacts with RSA reactivity to relate to externalizing problems in children. The flanker task was administered to 168 early elementary school-age children to assess their IIRTV, and the Child Behavior Checklist-Chinese version (CBCL-CV) was completed by their parents to assess children's externalizing problems. Electrocardiogram data were collected during the resting and task period to calculate resting RSA and RSA reactivity. The results indicated that children's IIRTV was correlated with their externalizing problems. Importantly, IIRTV interacted with RSA reactivity to relate to externalizing problems. IIRTV was positively related to externalizing problems in children with RSA suppression but was not significantly related to externalizing problems in children with RSA augmentation during the task. These findings suggest that poor attentional or cognitive control, as indexed by greater IIRTV, is related to more externalizing problems. Moreover, during mild cognitive stress, greater RSA suppression might indicate poor physiological regulation, which is more likely to increase externalizing problems for children with poor attentional or cognitive control.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijpsycho.2020.08.001DOI Listing
November 2020

Over high or low dietary protein levels depressed the growth, TOR signaling, apoptosis, immune and anti-stress of abalone Haliotis discus hannai.

Fish Shellfish Immunol 2020 Nov 8;106:241-251. Epub 2020 Aug 8.

The Key Laboratory of Aquaculture Nutrition and Feeds (Ministry of Agriculture and Rural Affairs), The Key Laboratory of Mariculture (Ministry of Education), The Ocean University of China, Qingdao, 266003, China.

A 120-day feeding trial was conducted to investigate the effects of relative higher and lower dietary protein levels on the growth, immunity and anti-stress of abalone Haliotis discus hannai fed diets with 17.64% (low), 30.49% (normal) and 43.27% (high) of proteins, respectively. The results showed that compared with 30.49% of dietary protein, 17.64% and 43.27% of dietary protein levels significantly decreased the weight gain rate and the activities of α-amylase, trypsin, alanine aminotransferase and aspartate aminotransferase in the hepatopancreas and serum of abalone (P < 0.05). Abalone fed 30.49% of dietary protein had the highest activity of superoxidase, acid phosphatase, alkaline phosphatase, lysozyme and the total anti-oxidative capacity, and the lowest content of malondialdehyde in the serum and hepatopancreas (P < 0.05). The gene expressions of TOR, S6k, Bcl-2, IκB, NfκB, TNF-α and Nrf were significantly up-regulated in the group with 30.49% of dietary protein (P < 0.05). Pathological abnormalities in hepatocyte cells of abalone were found in the groups with 17.64% and 43.27% of dietary protein. Meanwhile, accumulative mortalities of abalone after the Vibrio parahaemolyticus challenge test and heat stress test were significantly increased within these two groups (P < 0.05). In conclusion, the excessive (43.27) or deficient (17.64) dietary protein levels depressed the growth and immunity of abalone. Combined with the stress tests results, 17.63% or 43.27% of dietary protein contents are not recommended to the abalone facing the stress of vibriosis or high-water temperature (≥28 °C).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.fsi.2020.08.004DOI Listing
November 2020

Linewidth-related residual intensity modulation in lithium niobate phase modulators.

Appl Opt 2020 May;59(15):4739-4743

We present a modified model for residual intensity modulation (RIM) observed in lithium niobate phase modulators, which is suitable for both narrow linewidth and wide linewidth lasers. This model is based on two key points leading to RIM: one is the optical propagation loss, which is proportional to the applied voltage, and the other is the interference between an injected wave and its reflection from the lithium niobate substrate. In order to verify the model, the RIM is measured accurately with different linewidths of input lasers respectively. The experimental results are in good agreement with the theoretical model as the values of fitting determination coefficient R-square are all above 0.995. The results have revealed that the chief reasons causing RIM are different. When using a narrow linewidth laser, the interference is the dominant reason leading to RIM as the ratio of the reflection-related coefficient including linewidth effects to optical loss reaches 34.33. However, the optical loss is the dominant reason leading to RIM with the ratio mentioned above reaching 0.31 when using a wide linewidth laser.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1364/AO.388681DOI Listing
May 2020

Crystalline Red Phosphorus Nanoribbons: Large-Scale Synthesis and Electrochemical Nitrogen Fixation.

Angew Chem Int Ed Engl 2020 Aug 9;59(34):14383-14387. Epub 2020 Jul 9.

Shenzhen Engineering Center for the Fabrication of Two-Dimensional Atomic Crystals, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, P. R. China.

Two dimensional (2D) nanoribbons constitute an emerging nanoarchitecture for advanced microelectronics and energy conversion due to the stronger size confinement effects compared to traditional nanosheets. Triclinic crystalline red phosphorus (cRP) composed by a layered structure is a promising 2D phosphorus allotrope and the tube-like substructure is beneficial to the construction of nanoribbons. In this work, few-layer cRP nanoribbons are synthesized and the effectiveness in the electrochemical nitrogen reduction reaction (NRR) is investigated. An iodine-assisted chemical vapor transport (CVT) method is developed to synthesize circa 10 g of bulk cRP lumps with a yield of over 99 %. With the aid of probe ultrasonic treatment, high-quality cRP microcrystals are exfoliated into few-layer nanoribbons (cRP NRs) with large aspect ratios. As non-metallic materials, cRP NRs are suitable for the electrochemical nitrogen reduction reaction. The ammonia yield is 15.4 μg h  mg at -0.4 V vs. reversible hydrogen electrode in a neutral electrolyte under ambient conditions and the Faradaic efficiency is 9.4 % at -0.2 V. Not only is cRP a promising catalyst, but also the novel strategy expands the application of phosphorus-based 2D structures beyond that of traditional nanosheets.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/anie.202006679DOI Listing
August 2020

Interferometric optical gyroscope based on an integrated silica waveguide coil with low loss.

Opt Express 2020 May;28(10):15718-15730

An interferometric optical gyro (IOG) based on integrated devices are a promising alternative for miniaturized inertial sensors. However, improving their accuracy, which is determined by the sensing coil insertion loss, is crucial. In this work, an IOG is built using an integrated sensing coil produced from a 2.14-m-long SiO waveguide, the minimum bend radius and spacing of which are chosen to minimize the sensing coil insertion loss. The coil length is chosen by considering optimal detection limit constraints. Sinusoidal wave biasing modulation improves the system detection sensitivity. Finally, the IOG realizes the best yet reported bias drift of 7.32°/h.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1364/OE.392510DOI Listing
May 2020

Black Phosphorus Based Multicolor Light-Modulated Transparent Memristor with Enhanced Resistive Switching Performance.

ACS Appl Mater Interfaces 2020 Jun 20;12(22):25108-25114. Epub 2020 May 20.

Shenzhen Engineering Center for the Fabrication of Two-Dimensional Atomic Crystals, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, P. R. China.

Light-modulated transparent memristors combining photoresponse and data storage are promising as multifunctional devices. Herein, a multicolor light-modulated transparent memristor based on black phosphorous (BP) is designed, fabricated, and investigated. BP is a class of emerging two-dimensional (2D) materials with a natural direct band gap and a broad light absorption. Herein, BP nanosheets ([email protected] NSs) coated with polystyrene (PS) are prepared and serve as the resistive switching (RS) layer in the ITO/[email protected]/ITO memristor, which shows >75% transmittance between 350 and 1100 nm. With the aid of PS, the [email protected] memristor has excellent RS characteristics such as no initial preforming, low operating voltage, and long retention time. According to the energy band model, the RS mechanism of the high and low resistance states contributes to the transformation from ohmic contact to Schottky contact. During light illumination ranging from ultraviolet (380 nm) to near infrared (785 nm), the Schottky barrier height is elevated further so that the resetting voltages and power consumption decrease. Moreover, the ON/OFF ratios are improved and the maximum enhancement is demonstrated to be more than 10 times. BP is a promising RS material in light-modulated memristors, and the novel device configuration provides insights into the development of multifunctional microelectronic devices based on 2D materials.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.0c04493DOI Listing
June 2020

Large-scale identification of expression quantitative trait loci in Arabidopsis reveals novel candidate regulators of immune responses and other processes.

J Integr Plant Biol 2020 Oct 4;62(10):1469-1484. Epub 2020 May 4.

Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, Indiana, 47907, USA.

The extensive phenotypic diversity within natural populations of Arabidopsis is associated with differences in gene expression. Transcript levels can be considered as inheritable quantitative traits, and used to map expression quantitative trait loci (eQTL) in genome-wide association studies (GWASs). In order to identify putative genetic determinants for variations in gene expression, we used publicly available genomic and transcript variation data from 665 Arabidopsis accessions and applied the single nucleotide polymorphism-set (Sequence) Kernel Association Test (SKAT) method for the identification of eQTL. Moreover, we used the penalized orthogonal-components regression (POCRE) method to increase the power of statistical tests. Then, gene annotations were used as test units to identify genes that are associated with natural variations in transcript accumulation, which correspond to candidate regulators, some of which may have a broad impact on gene expression. Besides increasing the chances to identify real associations, the analysis using POCRE and SKAT significantly reduced the computational cost required to analyze large datasets. As a proof of concept, we used this approach to identify eQTL that represent novel candidate regulators of immune responses. The versatility of this approach allows its application to any process that is subjected to natural variation among Arabidopsis accessions.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/jipb.12930DOI Listing
October 2020

Visual Field Changes Over 5 Years in Patients Treated With Panretinal Photocoagulation or Ranibizumab for Proliferative Diabetic Retinopathy.

JAMA Ophthalmol 2020 03;138(3):285-293

Beetham Eye Institute, Joslin Diabetes Center, Department of Ophthalmology, Harvard, Boston, Massachusetts.

Importance: Preservation of peripheral visual field (VF) is considered an advantage for anti-vascular endothelial growth factor agents compared with panretinal photocoagulation (PRP) for treatment of proliferative diabetic retinopathy. Long-term data on VF are important when considering either treatment approach.

Objective: To further evaluate changes in VF throughout 5 years among eyes enrolled in the Protocol S clinical trial, conducted by the DRCR Retina Network.

Design, Setting, And Participants: Post hoc analyses of an ancillary study within a multicenter (55 US sites) randomized clinical trial. Individuals with eyes with proliferative diabetic retinopathy enrolled in Protocol S were included. Data were collected from February 2012 to February 2018. Analysis began in June 2018.

Interventions: Panretinal photocoagulation or intravitreous injections of 0.5-mg ranibizumab. Diabetic macular edema, whenever present, was treated with ranibizumab in both groups. Panretinal photocoagulation could be administered to eyes in the ranibizumab group when failure or futility criteria were met.

Main Outcomes And Measures: Mean change in total point score on VF testing with the Humphrey Field Analyzer 30-2 and 60-4 test patterns.

Results: Of 394 eyes enrolled in Protocol S, 234 (59.4%) were targeted for this ancillary study. Of these, 167 (71.4%) had VF meeting acceptable quality criteria at baseline (median [interquartile range] age, 50 [43-58] years; 90 men [53.9%]). At 5 years, 79 (33.8%) had results available. The mean (SD) change in total point score in the PRP and ranibizumab groups was -305 (521) dB and -36 (486) dB at 1 year, respectively, increasing to -527 (635) dB and -330 (645) dB at 5 years, respectively (P = .04). After censoring VF results after PRP treatments in the ranibizumab group, the 5-year mean change in total point score was -201 (442) dB. In a longitudinal regression analysis of change in total point score including both treatment groups, laser treatment was associated with a mean point decrease of 208 (95% CI, 112-304) dB for the initial PRP session, 77 (95% CI, 21-132) dB for additional PRP sessions, and 325 (95% CI, 211-439) dB for endolaser. No association was found between change in point score and the number of ranibizumab injections during the previous year (-9 per injection [95% CI, -22 to 3]).

Conclusions And Relevance: The limited data available from Protocol S suggest that there are factors besides PRP associated with VF loss in eyes treated for proliferative diabetic retinopathy. Further clinical research is warranted to clarify the finding.

Trial Registration: ClinicalTrials.gov identifier: NCT01489189.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1001/jamaophthalmol.2019.5939DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7042909PMC
March 2020

Chinese college students' parental attachment, peer attachment, and prosocial behaviors: The moderating role of respiratory sinus arrhythmia.

Biol Psychol 2020 02 15;150:107844. Epub 2020 Jan 15.

Shaanxi Normal University, Shaanxi Provincial Key Research Center of Child Mental and Behavioral Health, No. 199, South Chang'an Road, Yanta District, Xi'an 710062, China. Electronic address:

The goal of this study was to examine whether the links between Chinese college students' parental attachment, peer attachment, and prosocial behaviors were moderated by a physiological factor-baseline respiratory sinus arrhythmia (RSA). The simplified version of the Inventory of Parental and Peer Attachment (IPPA-R) and the Chinese version of the Prosocial Tendencies Measure (PTM-R) were administered to one hundred forty-four undergraduate students (M = 18.96 years, SD = 1.06 years; 30 % men) to assess parental and peer attachment and prosocial behaviors. Baseline RSA was calculated by electrocardiogram (ECG) data collected during a resting period in the laboratory. The results showed that both parental and peer attachment were positively associated with college students' global prosocial behaviors. Moreover, peer attachment interacted with baseline RSA to predict college students' global prosocial behaviors. Specifically, peer attachment was positively related to global prosocial behaviors among college students with low baseline RSA, while peer attachment was not related to global prosocial behaviors among college students with high baseline RSA. In addition, the examination with submeasures of prosocial behaviors revealed that parental and peer attachment and their interaction with baseline RSA have different effects on these different types of prosocial behaviors. The current findings highlight the importance of the consideration of psychosocial factors in conjunction with physiological factors to predict college students' prosocial behaviors.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biopsycho.2020.107844DOI Listing
February 2020

Rapid Activation of Platinum with Black Phosphorus for Efficient Hydrogen Evolution.

Angew Chem Int Ed Engl 2019 Dec 6;58(52):19060-19066. Epub 2019 Nov 6.

Materials and Interfaces Center, Institute of Advanced Materials Science and Engineering, Chinese Academy of Sciences, Shenzhen, 518055, P. R. China.

Modulation of the electronic structure of metal catalysts is an effective approach to optimize the electrocatalytic activity. Herein, we show a surprisingly strong activation effect of black phosphorus (BP) on platinum (Pt) catalysts to give greatly enhanced catalytic activity in the hydrogen evolution reaction (HER). The unique and negative binding energy between BP and Pt leads to spontaneous formation of Pt-P bonds producing strong synergistic ligand effects on the Pt nanoparticles. No Pt-P bonds are formed with red phosphorus which is another allotrope of P. By controlling the number of Pt-P bonds, 3.5-fold enhancement in the HER activity can be achieved from the BP-activated Pt catalyst and the activity is 6.1 times higher than that of the state-of-the-art commercial Pt/C catalyst. The BP-activated Pt catalyst exhibits a current density of 82.89 mA cm with only 1 μg of Pt in 1 m KOH at an overpotential of 70 mV.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/anie.201911696DOI Listing
December 2019

Modulation of Phosphorene for Optimal Hydrogen Evolution Reaction.

ACS Appl Mater Interfaces 2019 Oct 4;11(41):37787-37795. Epub 2019 Oct 4.

Shenzhen Institutes of Advanced Technology , Chinese Academy of Sciences , Shenzhen 518055 , P. R. China.

Economical and highly effective catalysts are crucial to the electrocatalytic hydrogen evolution reaction (HER), and few-layer black phosphorus (phosphorene) is a promising candidate because of the high carrier mobility, large specific surface area, and tunable physicochemical characteristics. However, the HER activity of phosphorene is limited by the weak hydrogen adsorption ability on the basal plane. In this work, optimal active sites are created to modulate the electronic structure of phosphorene to improve the HER activity and the effectiveness is investigated theoretically by density-functional theory calculation and verified experimentally. The edges and defects affect the electronic density of states, and a linear relationship between the HER activity and lowest unoccupied states (ε) is discovered. The medium ε value corresponds to the suitable hydrogen adsorption strength. Experiments are designed and performed to verify the prediction, and our results show that a smaller phosphorene moiety with more edges and defects exhibits better HER activity and surface doping with metal adatoms improves the catalytic performance. The results suggest that modified phosphorene has large potential in efficient HER and provides a convenient standard to explore ideal electrocatalysts.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.9b13666DOI Listing
October 2019

Facile mass production of self-supported two-dimensional transition metal oxides for catalytic applications.

Chem Commun (Camb) 2019 Sep 4;55(76):11406-11409. Epub 2019 Sep 4.

Material Interfaces Center, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.

We report a novel metal-corrosion route beyond traditional top-down or bottom-up strategies for the mass production of 2D TMOs with a self-supported structure. The self-supported 2D CoO, a typical TMO, exhibits excellent electrocatalytic activity and stability in the oxygen evolution reaction surpassing those of commercial precious metal RuO catalysts at high currents.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/c9cc06261kDOI Listing
September 2019

An amperometric biosensor based on [email protected] nanocomposites for the detection of galectin-1 via lactose-galectin interactions.

Nanotechnology 2019 11 29;30(48):485706. Epub 2019 Nov 29.

Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, People's Republic of China. Department of Medical Laboratory, The Second Clinical Medical College (Shenzhen People's Hospital), Jinan University, Shenzhen 518020, People's Republic of China.

In this work, a novel label-free electrochemical biosensor is developed for the detection of galectin-1 (Gal-1) based on gold nanoparticle (AuNP) loaded octahedral CuO ([email protected]) nanocomposites. The AuNPs on the surface of the CuO nanocrystals not only enhance the electrochemical performance, but also serve as the binding sites for the lactose ligand which can specifically bind with Gal-1. The [email protected] nanocomposites provide the synergic effect of electrochemical signal amplification and lactose-galectin reaction as the recognition strategy. Under optimal conditions, the proposed biosensor exhibits a variation of electrochemical responses to different concentrations of Gal-1 ranging from 0.1 pg ml to 10 ng ml. This work presents an alternative electrochemical biosensor for the detection of tumor biomarkers based on a simple and economical lactose ligand incorporated [email protected] biosensor platform.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1088/1361-6528/ab3cdeDOI Listing
November 2019

Two-Step Maternal-to-Zygotic Transition with Two-Phase Parental Genome Contributions.

Dev Cell 2019 06 9;49(6):882-893.e5. Epub 2019 May 9.

State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, China. Electronic address:

The maternal-to-zygotic transition (MZT) is an essential developmental turning point in both plants and animals. In plants, the timing of MZT and parental contributions to the zygotic transcriptome remain unclear. Here, by overcoming technical limitations, we characterize the Arabidopsis egg cell, zygote, and embryo transcriptomes across multiple stages. Using these datasets, we demonstrate that MZT occurs during zygote development and is a two-step interrelated process of rapid maternal transcript degradation followed by large-scale de novo transcription. Parental contributions to the zygotic transcriptome are stage-dependent: the spherical zygote is characterized by a maternally dominated transcriptome, whereas the elongated zygote transcriptome shows equal parental contributions. Our results show that plant MZT is similar to that in animals, showing a typical two-step process, and that zygotic genome activation is required for zygote elongation and division, indicating that de novo transcripts are essential for the establishment of zygote polarity and embryogenesis promotion.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.devcel.2019.04.016DOI Listing
June 2019

Protective effects of Rosavin on bleomycin-induced pulmonary fibrosis via suppressing fibrotic and inflammatory signaling pathways in mice.

Biomed Pharmacother 2019 Jul 23;115:108870. Epub 2019 Apr 23.

Key Laboratory of Xinjiang Phytomedicine Resource and Utilization, Ministry of Education, College of Pharmacy, Shihezi University, Shihezi, 832002, China; Shenzhen Honghui Biopharmaceutical Co., Ltd. Shenzhen 518000, China. Electronic address:

Idiopathic Pulmonary fibrosis (IPF) is diagnosed as a life-threatening, progressive and incurable lung disease characterized by accumulation of extracellular matrix and myofibroblasts, resulting in the function degradation and structural alterations in normal lung parenchyma. Notably, Pulmonary Fibrosis has been considering as a difficult problem in clinical with high mortality and effective treatment strategies. Rosavin, a benzylPropylene glycoside, is isolated from Rhodiola rosea L., exhibiting nootropic, anti-depressant, anti-cancer, anti-inflammatory and anti-oxidative activities. In this study, we attended to elucidate the pharmacological activity of Rosavin for treatment of pulmonary fibrosis induced by bleomycin in mice. The results indicated that Rosavin could significantly ameliorate the lung index and Pathological structure of mice with Pulmonary fibrosis by bleomycin-induced. Additionally, Rosavin could evidently decreased inflammatory cells infiltration in bronchoalveolar lavage fluid and pro-inflammatory cytokines expression in lung tissue specimens induced by bleomycin. Rosavin could down-regulate the expression of hydroxyproline and malondialdehyde and increased the activities of superoxide dismutase, glutathione peroxidase in lung tissue. The expression of Nrf2 were increased, and the expression of NF-κB p65, TGF-β1 and α-SMA were inhibited. The findings revealed the protective effects and the primary mechanism of rosavin on bleomycin-induced pulmonary fibrosis, which provided a scientific foundation for Rosavin as a promising candidate for Pulmonary fibrosis treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biopha.2019.108870DOI Listing
July 2019

Modification of Layered Graphitic Carbon Nitride by Nitrogen Plasma for Improved Electrocatalytic Hydrogen Evolution.

Nanomaterials (Basel) 2019 Apr 8;9(4). Epub 2019 Apr 8.

Department of Physics and Department of Materials Science and Engineering, City University of Hong Kong Tat Chee Avenue, Kowloon 999077, Hong Kong, China.

As a layered nano-sheet material, layered graphitic carbon nitride (g-C₃N₄) has attracted attention in multifunctional photocatalytic applications. However, g-C₃N₄ is electrochemically inert consequently hampering electrochemical applications. In this work, low-temperature nitrogen plasma processing was conducted to modify g-C₃N₄ to enhance the electrocatalytic performance in the hydrogen evolution reaction (HER). The plasma produced significant morphological and chemical changes on the surface of g-C₃N₄ via active species, and nitrogen atoms were incorporated into the surface while the bulk properties did not change. The modification improved the surface hydrophilicity and electrocatalytic HER activity, as well as excellent stability in HER after 2000 cycles. Our results revealed that plasma treatment was a promising technique to improve the HER of carbon-based layered nano-sheet materials.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/nano9040568DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6523230PMC
April 2019

Template growth of Au/Ag nanocomposites on phosphorene for sensitive SERS detection of pesticides.

Nanotechnology 2019 Jul 25;30(27):275604. Epub 2019 Mar 25.

Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, People's Republic of China. Department of Physics and Department of Materials Science and Engineering, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong, People's Republic of China.

We report template growth and dense packing of noble metallic nanoparticles (NPs) on few-layer phosphorene for sensitive surface-enhanced Raman scattering (SERS) detection. Phosphorene obtained by electrochemical exfoliation serves as both the template and reductant in the fabrication of noble metallic NPs which are dispersed on phosphorene without aggregation or pile-up. The BP/Ag/Au and BP/Au/Ag nanocomposites with a nanogap structure exhibit excellent SERS sensitivity and reproducibility with respect to Rhodamine 6G. The BP/Au/Ag nanocomposite is further utilized as the SERS substrate for the detection of two fungicides, thiabendazole and thiram, and detection limits of 10 and 10 M are achieved. Template growth of Au/Ag nanocomposites is demonstrated to be a green strategy for the fabrication of SERS substrates and has large potential in applications such as pesticide detection.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1088/1361-6528/ab12fbDOI Listing
July 2019

Hybrid sequencing reveals insight into heat sensing and signaling of bread wheat.

Plant J 2019 06 23;98(6):1015-1032. Epub 2019 Apr 23.

State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Sciences, Northwest A&F University, Yangling, 712100, Shaanxi, China.

Wheat (Triticum aestivum L.), a globally important crop, is challenged by increasing temperatures (heat stress, HS). However its polyploid nature, the incompleteness of its genome sequences and annotation, the lack of comprehensive HS-responsive transcriptomes and the unexplored heat sensing and signaling of wheat hinder our full understanding of its adaptations to HS. The recently released genome sequences of wheat, as well as emerging single-molecular sequencing technologies, provide an opportunity to thoroughly investigate the molecular mechanisms of the wheat response to HS. We generated a high-resolution spatio-temporal transcriptome map of wheat flag leaves and filling grain under HS at 0 min, 5 min, 10 min, 30 min, 1 h and 4 h by combining full-length single-molecular sequencing and Illumina short reads sequencing. This hybrid sequencing newly discovered 4947 loci and 70 285 transcripts, generating the comprehensive and dynamic list of HS-responsive full-length transcripts and complementing the recently released wheat reference genome. Large-scale analysis revealed a global landscape of heat adaptations, uncovering unexpected rapid heat sensing and signaling, significant changes of more than half of HS-responsive genes within 30 min, heat shock factor-dependent and -independent heat signaling, and metabolic alterations in early HS-responses. Integrated analysis also demonstrated the differential responses and partitioned functions between organs and subgenomes, and suggested a differential pattern of transcriptional and alternative splicing regulation in the HS response. This study provided comprehensive data for dissecting molecular mechanisms of early HS responses in wheat and highlighted the genomic plasticity and evolutionary divergence of polyploidy wheat.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/tpj.14299DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6850178PMC
June 2019

A Low-Cost Metal-Free Photocatalyst Based on Black Phosphorus.

Adv Sci (Weinh) 2019 Jan 9;6(1):1801321. Epub 2018 Nov 9.

Center for Biomedical materials and Interfaces Shenzhen Institutes of Advanced Technology Chinese Academy of Sciences Shenzhen 518055 P. R. China.

An efficient metal-free photocatalyst composed of black phosphorus (BP) and graphitic carbon nitride (CN) is prepared on a large scale by ball milling. Using economical urea and red phosphorus (RP) as the raw materials, the estimated materials cost of BP/CN is 0.235 Euro per gram. The BP/CN heterostructure shows efficient charge separation and possesses abundant active sites, giving rise to excellent photocatalytic H evolution and rhodamine B (RhB) degradation efficiency. Without using a co-catalyst, the metal-free BP/CN emits H consistently at a rate as large as 786 µmol h g and RhB is decomposed in merely 25 min during visible-light irradiation. The corresponding electron/hole transfer and catalytic mechanisms are analyzed and described. The efficient metal-free catalyst is promising in visible-light photocatalysis and the simple ball-milling synthetic method can be readily scaled up.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/advs.201801321DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6325597PMC
January 2019

Self-assembled GFFYK peptide hydrogel enhances the therapeutic efficacy of mesenchymal stem cells in a mouse hindlimb ischemia model.

Acta Biomater 2019 02 11;85:94-105. Epub 2018 Dec 11.

Nankai University School of Medicine, Tianjin 300071, China; The Key Laboratory of Bioactive Materials, Ministry of Education, Nankai University, The College of Life Science, Tianjin 300071, China. Electronic address:

Mesenchymal stem cell (MSC) transplantation has emerged as a very promising strategy for the treatments of peripheral artery disease (PAD). However, MSC-based therapies are limited by low cell retention and survival rate in the ischemic zone. Small molecular (SM) hydrogels have shown attractive abilities to enhance the therapeutic effects of human MSCs via promoting their proliferation or maintaining their differentiation potential. Here, we designed and synthesized a new bioactive and biocompatible hydrogel, Nap-GFFYK-Thiol, using disulfide bonds as cleavable linkers to control the molecular self-assembly and we hypothesized this hydrogel could enhance the retention and engraftment of human placenta-derived MSCs (hP-MSCs) in a mouse ischemic hindlimb model. In vitro results demonstrated that the Nap-GFFYK-Thiol hydrogel increased cell viability through paracrine effects. Moreover, it enhanced the proangiogenic and anti-apoptotic effects of hP-MSCs. In vivo, Nap-GFFYK-Thiol hydrogel improved the hP-MSC retention in the murine ischemic hindlimb model as visualized by bioluminescence imaging. Furthermore, cotransplantation of hP-MSCs with hydrogel improved blood perfusion, leading to superior limb salvage. These therapeutic effects may attribute to reduced inflammatory cell infiltration, enhanced angiogenesis as well as suppressed collagen deposition. In conclusion, the Nap-GFFYK-Thiol hydrogel fabricated using disulfide bonds as cleavable linkers serves as an artificial niche for promoting hP-MSC survival and proangiogenic factor secretion in PAD therapy and thereby provide an alternative strategy for PAD therapy. STATEMENT OF SIGNIFICANCE: Although several phase I/II clinical trials of MSC-based treatments for critical limb ischemia (CLI) are ongoing, MSC-based therapies are still challenged by the low quality and quantity of cells in the ischemic zone, especially in cases of extensive or irreversible damage. Hydrogels have favorable biocompatibility and safety records in the medical field. In the current study, we engineered a new bioactive and biocompatible hydrogel, Nap-GFFYK-Thiol, using disulfide bonds as cleavable linkers to enhance the therapeutic efficacy of human placenta-derived MSCs (hP-MSCs) in mouse limb ischemia model. Notably, Nap-GFFYK-Thiol hydrogel acts as an artificial niche for promoting hP-MSC survival and proangiogenic factor secretion in PAD therapy, which further promoted the restoration of blood perfusion and regeneration of muscle cells. Considering the proangiogenic effect of Nap-GFFYK-Thiol on hP-MSCs, our results may provide a new strategy for the treatment of PAD.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.actbio.2018.12.015DOI Listing
February 2019

Reply.

Ophthalmology 2018 11;125(11):e82

Feinberg School of Medicine, Northwestern University, Chicago, Illinois.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ophtha.2018.05.004DOI Listing
November 2018

Five-Year Outcomes of Panretinal Photocoagulation vs Intravitreous Ranibizumab for Proliferative Diabetic Retinopathy: A Randomized Clinical Trial.

JAMA Ophthalmol 2018 10;136(10):1138-1148

Jaeb Center for Health Research, Tampa, Florida.

Importance: Ranibizumab is a viable treatment option for eyes with proliferative diabetic retinopathy (PDR) through 2 years. However, longer-term results are needed.

Objective: To evaluate efficacy and safety of 0.5-mg intravitreous ranibizumab vs panretinal photocoagulation (PRP) over 5 years for PDR.

Design, Setting, And Participants: Diabetic Retinopathy Clinical Research Network multicenter randomized clinical trial evaluated 394 study eyes with PDR enrolled February through December 2012. Analysis began in January 2018.

Interventions: Eyes were randomly assigned to receive intravitreous ranibizumab (n = 191) or PRP (n = 203). Frequency of ranibizumab was based on a protocol-specified retreatment algorithm. Diabetic macular edema could be managed with ranibizumab in either group.

Main Outcomes And Measures: Mean change in visual acuity (intention-to-treat analysis) was the main outcome. Secondary outcomes included peripheral visual field loss, development of vision-impairing diabetic macular edema, and ocular and systemic safety.

Results: The 5-year visit was completed by 184 of 277 participants (66% excluding deaths). Of 305 enrolled participants, the mean (SD) age was 52 (12) years, 135 (44%) were women, and 160 (52%) were white. For the ranibizumab and PRP groups, the mean (SD) number of injections over 5 years was 19.2 (10.9) and 5.4 (7.9), respectively; the mean (SD) change in visual acuity letter score was 3.1 (14.3) and 3.0 (10.5) letters, respectively (adjusted difference, 0.6; 95% CI, -2.3 to 3.5; P = .68); the mean visual acuity was 20/25 (approximate Snellen equivalent) in both groups at 5 years. The mean (SD) change in cumulative visual field total point score was -330 (645) vs -527 (635) dB in the ranibizumab (n = 41) and PRP (n = 38) groups, respectively (adjusted difference, 208 dB; 95% CI, 9-408). Vision-impairing diabetic macular edema developed in 27 and 53 eyes in the ranibizumab and PRP groups, respectively (cumulative probabilities: 22% vs 38%; hazard ratio, 0.4; 95% CI, 0.3-0.7). No statistically significant differences between groups in major systemic adverse event rates were identified.

Conclusions And Relevance: Although loss to follow-up was relatively high, visual acuity in most study eyes that completed follow-up was very good at 5 years and was similar in both groups. Severe vision loss or serious PDR complications were uncommon with PRP or ranibizumab; however, the ranibizumab group had lower rates of developing vision-impairing diabetic macular edema and less visual field loss. Patient-specific factors, including anticipated visit compliance, cost, and frequency of visits, should be considered when choosing treatment for patients with PDR. These findings support either anti-vascular endothelial growth factor therapy or PRP as viable treatments for patients with PDR.

Trial Registration: ClinicalTrials.gov Identifier: NCT01489189.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1001/jamaophthalmol.2018.3255DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6233839PMC
October 2018
-->