Publications by authors named "Danjie Zhu"

13 Publications

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[Development of on-line lateral stiffness measurement system for anterior cruciate ligament and its influence on anterior cruciate ligament reconstruction].

Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 2021 Feb;38(1):145-153

Sports Medicine and Joint Surgery Division, Zhejiang Provincial People's Hospital, Hangzhou 310014, P.R.China.

The anterior cruciate ligament (ACL) reconstruction mostly relies on the experience of surgeons. To improve the effectiveness and adaptability of the tension after ACL reconstruction in knee joint rehabilitation, this paper establishes a lateral force measurement model with relaxation characteristics and designs an on-line stiffness measurement system of ACL. In this paper, we selected 20 sheep knee joints as experimental material for the knee joint stability test before the ACL reconstruction operation, which were divided into two groups for a comparative test of single-bundle ACL reconstruction through the anterolateral approach. The first group of surgeons carried out intraoperative detection with routine procedures. The second group used ACL on-line stiffness measurement system for intraoperative detection. After that, the above two groups were tested for postoperative stability. The study results show that the tension accuracy is (- 2.3 ± 0.04)%, and the displacement error is (1.5 ± 1.8)%. The forward stability, internal rotation stability, and external rotation stability of the two groups were better than those before operation ( < 0.05). But the data of the group using the system were closer to the preoperative knee joint measurement index, and there was no significant difference between them ( > 0.05). The system established in this paper is expected to help clinicians judge the ACL reconstruction tension in the operation process and effectively improve the surgical effect.
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http://dx.doi.org/10.7507/1001-5515.202006060DOI Listing
February 2021

Identification and Validation of the Prognostic Stemness Biomarkers in Bladder Cancer Bone Metastasis.

Front Oncol 2021 19;11:641184. Epub 2021 Mar 19.

Department of Orthopedics, Zhejiang Provincial People's Hospital, Hangzhou, China.

Background: Bladder urothelial carcinoma (BLCA) is one of the most common urinary system malignancies with a high metastasis rate. Cancer stem cells (CSCs) play an important role in the occurrence and progression of BLCA, however, its roles in bone metastasis and the prognostic stemness biomarkers have not been identified in BLCA.

Method: In order to identify the roles of CSC in the tumorigenesis, bone metastasis and prognosis of BLCA, the RNA sequencing data of patients with BLCA were retrieved from The Cancer Genome Atlas (TCGA) databases. The mRNA expression-based stemness index (mRNAsi) and the differential expressed genes (DEGs) were evaluated and identified. The associations between mRNAsi and the tumorigenesis, bone metastasis, clinical stage and overall survival (OS) were also established. The key prognostic stemness-related genes (PSRGs) were screened by Lasso regression, and based on them, the predict model was constructed. Its accuracy was tested by the area under the curve (AUC) of the receiver operator characteristic (ROC) curve and the risk score. Additionally, in order to explore the key regulatory network, the relationship among differentially expressing TFs, PSRGs, and absolute quantification of 50 hallmarks of cancer were also identified by Pearson correlation analysis. To verify the identified key TFs and PSRGs, their expression levels were identified by our clinical samples immunohistochemistry (IHC).

Results: A total of 8,647 DEGs were identified between 411 primary BLCAs and 19 normal solid tissue samples. According to the clinical stage, mRNAsi and bone metastasis of BLCA, 2,383 stage-related DEGs, 3,680 stemness-related DEGs and 716 bone metastasis-associated DEGs were uncovered, respectively. Additionally, compared with normal tissue, mRNAsi was significantly upregulated in the primary BLCA and also associated with the prognosis (P = 0.016), bone metastasis (P < 0.001) and AJCC clinical stage (P < 0.001) of BLCA patients. A total of 20 PSRGs were further screened by Lasso regression, and based on them, we constructed the predict model with a relatively high accuracy (AUC: 0.699). Moreover, we found two key TFs (EPO, ARID3A), four key PRSGs (CACNA1E, LINC01356, CGA and SSX3) and five key hallmarks of cancer gene sets (DNA repair, myc targets, E2F targets, mTORC1 signaling and unfolded protein response) in the regulatory network. The tissue microarray of BLCA and BLCA bone metastasis also revealed high expression of the key TFs (EPO, ARID3A) and PRSGs (SSX3) in BLCA.

Conclusion: Our study identifies mRNAsi as a reliable index in predicting the tumorigenesis, bone metastasis and prognosis of patients with BLCA and provides a well-applied model for predicting the OS for patients with BLCA based on 20 PSRGs. Besides, we also identified the regulatory network between key PSRGs and cancer gene sets in mediating the BLCA bone metastasis.
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http://dx.doi.org/10.3389/fonc.2021.641184DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8017322PMC
March 2021

A study of knee anterior cruciate ligament biomechanics with respect to energy and relaxation.

Clin Biomech (Bristol, Avon) 2020 12 29;80:105159. Epub 2020 Aug 29.

Department of Computer and Information Sciences, Virginia Military Institute, Lexington, VA, USA.

Background: This research aimed to study the biomechanical properties of sheep tendon under torsion and the tendon energy absorption performance with an externally imposed initial force.

Methods: Tendons of nine healthy knees of sheep were investigated. In both tests, we investigated energy and relaxation at rotations of 0°, 90°, 180°, and 360°. For both tensile force and tensile displacement at a sampling period of 100 milliseconds, the maximum value of 89 N was selected as the maximum tension state for 600 s of relaxation duration for testing relaxation, and analysed of the average force of the last 30 s.

Findings: The difference of energy levels of the tendons are significant between twisted groups (180° and 360°) and untwisted group (0°) (P < 0.05); The relaxation force decreases significantly with twisted groups (90°,180°, and 360°) and untwists group (0°) (P < 0.05). The nine-group tendons show no significant difference at torsion 90° and 180° (P = 0.466). Peak force test shows significant differences between the twisted groups (90°,180°, and 360°) and untwisted group (0°) (P < 0.05).

Interpretation: The torsion tendon has lower energy absorption and relaxation than the untwisted counterparts; thus, it may be more prone to damage. These results are useful for providing guidance on anterior cruciate ligament reconstruction.
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http://dx.doi.org/10.1016/j.clinbiomech.2020.105159DOI Listing
December 2020

miR-29a-5p Targets SATB2 and Regulates the SIRT1/Smad3 Deacetylation Pathway to Inhibit Thoracic Ligamentum Flavum Cell Osteogenesis.

Spine (Phila Pa 1976) 2020 Sep;45(17):E1057-E1065

Department of Orthopedics, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou, PR China.

Study Design: Experimental analysis of the thoracic ligamentum flavum cell osteogenic differentiation process.

Objective: This study aimed to explore the role of miR-29a-5p and special AT-rich sequence-binding protein 2 (SATB2) in a pathological osteogenic process.

Summary Of Background Data: Thoracic ossification of the ligamentum flavum (TOLF) is an uncommon disease wherein ligaments within the spine undergo progressive ossification, resulting in stenosis of the spinal canal and myelopathy. MiR-29a-5p was found to be downregulated in ligament cells from ossified ligament tissue in a previous study. However, whether miR-29a-5p is involved in the process of TOLF has not been investigated.

Methods: The expression of miR-29a-5p in ligament tissues or in the context of TOLF osteogenic cell differentiation was measured via qRT-PCR. Alkaline phosphatase activity assay and Alizarin red staining were used to analyze cellular osteogenesis. The protein-level expression of SATB2, SIRT1, and Smad3 were measured via immunohistochemistry or western blotting. Dual luciferase reporter assays and western blotting were used to confirm that miR-29a targets SATB2.

Results: SATB2 was found to be upregulated and miR-29a-5p was downregulated in TOLF tissue. We additionally observed decreased miR-29a-5p expression during the process of TOLF osteogenic cell differentiation, and there was a marked reduction in the expression of key mediators of osteogenesis when miR-29a-5p was overexpressed. Consistent with this, when miR-29a-5p was inhibited this led to enhanced osteogenic cell differentiation of these cells. We further found miR-29a-5p to directly target and suppress the expression of SATB2. Knock-down of SATB2 was sufficient to reduce the ability of miR-29a-5p to inhibit osteogenesis, and this also led to decreased SIRT1 expression and Smad3 acetylation.

Conclusion: Together our findings indicate that miR-29a-5p is able to prevent thoracic ligamentum flavum cell osteogenesis at least in part via targeting SATB2 and thereby suppressing the SIRT1/Smad3 deacetylation pathway.

Level Of Evidence: N/A.
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http://dx.doi.org/10.1097/BRS.0000000000003505DOI Listing
September 2020

High glucose promotes annulus fibrosus cell apoptosis through activating the JNK and p38 MAPK pathways.

Biosci Rep 2019 07 8;39(7). Epub 2019 Jul 8.

Department of Orthopaedic Surgery, Zhejiang Provincial People's Hospital, Hangzhou 310014, Zhejiang, China.

Diabetes mellitus (DM) is an important risk factor of intervertebral disc degeneration. A high glucose niche-mediated disc cell apoptosis is an implicate causative factor for the spine degenerative diseases related with DM. However, the effects of a high glucose niche on disc annulus fibrosus (AF) cell apoptosis and the potential signaling transduction pathway is unclear. The present study is to investigate the effects of high glucose on disc AF cell apoptosis and the role of two potential signaling pathways in this process. Rat AF cells were cultured in baseline medium or medium with different concentrations (0.1 and 0.2 M) of glucose for 3 days. Flow cytometry was used to assess the degree of apoptosis. Activity of caspase 3/9 was evaluated by chemical kit. Expression of pro-apoptotic and anti-apoptotic molecules was analyzed by real-time polymerase chain reaction and Western blot. In addition, activity of the C-Jun NH-terminal kinases (JNK) pathway and p38 mitogen-activated protein kinase (MAPK) pathway was evaluated by Western blot. Compared with the control group, high glucose culture increased cell apoptosis ratio and caspase-3/9 activity, up-regulated expression of bax, caspase-3, cleaved caspase-3 and cleaved PARP, and down-regulated expression of bcl-2 in a glucose concentration-dependent manner. Additionally, high glucose culture increased expression of the p-JNK and p-p38 MAPK in a concentration-dependent manner. Further results showed that inhibition of the JNK or p38 MAPK pathway attenuated the effects of high glucose on AF cell apoptosis. Together, high glucose promoted disc AF cell apoptosis through regulating the JNK pathway and p38 MAPK pathway in a glucose concentration-dependent manner.
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http://dx.doi.org/10.1042/BSR20190853DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6614572PMC
July 2019

Corrigendum to "17β-Estradiol/extrogen receptor β alleviates apoptosis and enhances matrix biosynthesis of nucleus pulposus cells through regulating oxidative damage under a high glucose condition" [Biomed. Pharmacother. 207 (2018) 1004-1009].

Biomed Pharmacother 2019 04 7;112:108739. Epub 2019 Mar 7.

Department of Orthopaedic Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, 215006, China. Electronic address:

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http://dx.doi.org/10.1016/j.biopha.2019.108739DOI Listing
April 2019

17β-Estradiol/extrogen receptor β alleviates apoptosis and enhances matrix biosynthesis of nucleus pulposus cells through regulating oxidative damage under a high glucose condition.

Biomed Pharmacother 2018 Nov 24;107:1004-1009. Epub 2018 Aug 24.

Department of Orthopaedic Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, 215006, China. Electronic address:

Background: Hyperglycemia in the Diabetes mellitus (DM) patients is a potential etiology of disc degeneration. 17β-estradiol (17β-E) supplementation plays an anti-diabetic role in DM patients.

Objective: This study was aimed to investigate the role and mechanism of 17β-E in regulating nucleus pulposus (NP) cell apoptosis and NP matrix production under a high glucose condition.

Methods: Rat NP cells were cultured in medium with a high glucose concentration (0.2 M). 17β-E was added into the culture medium to investigate its protective effects. The ERβ inhibitor PHTPP and ERβ activator ERB041 were used to investigate the effects of ERβ. NP cell apoptosis was analyzed by flow cytometry and expression of apoptosis-related molecules. NP matrix production was evaluated by expression of matrix macromolecules. Additionally, intracellular reactive oxygen species (ROS) content was also detected.

Results: Compared with the control NP cells, 17β-E decreased NP cell apoptosis ratio, down-regulated gene expression of Bax and caspase-3, up-regulated gene expression of Bcl-2, increased protein expression of cleaved PARP and cleaved caspase-3, and increased expression of matrix molecules (aggrecan and collagen II). Moreover, 17β-E decreased ROS content. Further analysis showed that ERβ inhibition partly reversed these effects of 17β-E whereas ERβ activation further promoted its effects.

Conclusion: 17β-E/ERβ interaction attenuates apoptosis and promotes matrix biosynthesis of NP cells through alleviating oxidative damage under a high glucose condition. This study provides new knowledge on strategies for retarding disc degeneration.
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http://dx.doi.org/10.1016/j.biopha.2018.08.084DOI Listing
November 2018

Heterogeneous expression and biological function of SOX18 in osteosaroma.

J Cell Biochem 2018 05 22;119(5):4184-4192. Epub 2018 Jan 22.

Department of Orthopedics, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou, China.

Osteosaroma (OS) is a primary bone malignancy and is associated with high morbidity. Sex determining region Y-box 18 (SOX18) is identified overexpressed in OS. However, the molecular mechanism underlying the biological function of SOX18 in OS is still unclear. The aim of the current study was to determine the SOX18 expression in patients with OS and its effect on tumor cell malignant phenotypes. Our results showed that SOX18 was overexpressed in OS patients from both E-MEXP-3628 database and independent samples from our hospital and in OS cell lines. SOX18 silencing significantly induced G0-G1 phase cell cycle arrest and apoptosis and inhibited U-2OS cell migration and invasion and cell growth both in vitro and in vivo. However, SOX18 overexpression remarkably promoted 143B cell proliferation, migration and invasion and inhibited cell cycle arrest and apoptosis. The protein expression levels of p53, p21, Bax, Bcl-2, and Caspase-3 were also regulated by SOX18. Moreover, SOX18 was found negative correlated with the expression of HERC1, HER2, HERC3, HERC4, HERC5, and HERC6 in OS patients and in OS cells, with the most significant correlation detected in HERC2 expression, which was following found interacted with SOX18 in OS cells. Taken together, our results suggest that SOX18 is overexpressed in OS and plays an important role in proliferation, apoptosis, migration and invasion of OS cells, and may provide a novel and promising thera-peutic strategy for OS.
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http://dx.doi.org/10.1002/jcb.26635DOI Listing
May 2018

Lumbar idiopathic intervertebral disc calcification associated with ossification of the ligamentum flavum in adult: a case report.

Authors:
Danjie Zhu Yu Chen

Br J Neurosurg 2018 Oct 13;32(5):579-581. Epub 2017 Jul 13.

a Department of Orthopaedics , Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College , Hangzhou , Zhejiang , China.

A 23 year-old female was diagnosed with lumbar idiopathic intervertebral disc calcification associated with ossification of OLF. The patient underwent posterior decompression and posterolateral fusion with pedicle screw fixation, which achieved excellent clinical improvement. Surgical intervention of a posterior approach for decompression and instrumented fusion is indicated in cases that the spinal cord compression with neurologic deficits was involved.
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http://dx.doi.org/10.1080/02688697.2017.1354120DOI Listing
October 2018

An Twist Fatigue Test of Fabric Stent-Grafts Supported by Z-Stents Ringed Stents.

Materials (Basel) 2016 Feb 16;9(2). Epub 2016 Feb 16.

Department of Surgery, Laval University and Axe of Regenerative Medicine, Centre de Recherche du CHU, Québec, QC G1V 0A6, Canada.

Whereas buckling can cause type III endoleaks, long-term twisting of a stent-graft was investigated here as a mechanism leading to type V endoleak or endotension. Two experimental device designs supported with Z-stents having strut angles of 35° or 45° were compared to a ringed control under accelerated twisting. Damage to each device was assessed and compared after different durations of twisting, with focus on damage that may allow leakage. Stent-grafts with 35° Z-stents had the most severe distortion and damage to the graft fabric. The 45° Z-stents caused less fabric damage. However, consistent stretching was still seen around the holes for sutures, which attach the stents to the graft fabric. Larger holes may become channels for fluid percolation through the wall. The ringed stent-graft had the least damage observed. Stent apexes with sharp angles appear to be responsible for major damage to the fabrics. Device manufacturers should consider stent apex angle when designing stent-grafts, and ensure their devices are resistant to twisting.
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http://dx.doi.org/10.3390/ma9020113DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5456472PMC
February 2016

FS-3D-FISP for the diagnosis of ankle impingement syndrome and the evaluation of clinical outcomes of arthroscopic surgery.

Eur J Orthop Surg Traumatol 2013 Oct 22;23(7):839-42. Epub 2012 Sep 22.

Department of Orthopedics and Joint Surgery, Zhejiang Provincial People's Hospital, Hangzhou, 310014, China.

This study aimed to assess the diagnostic value of three types of MRI sequences and to observe the clinical outcomes of arthroscopy surgery for ankle joint impingement syndrome. Ankle joint impingement syndrome was confirmed by FSE-T2WI, FSE-PDWI, and FS-3D-FISP MRI in 23 patients with arthroscopically proven ankle impingement. All 23 patients underwent arthroscopic surgery and the ankle joint function was evaluated before, 1 week after and 6 months after the operation. The patients were followed-up for 12-64 months (average 28 months). There was no significant difference in ankle function score between preoperatively and 1 week postoperatively, but 86.96 % patients got overall excellent or good scores 6 months after the surgery, significantly higher than before the surgery. The FS-3D-FISP MRI exhibited a good consistency with arthroscopic examination and had higher sensitivity and specificity for the diagnosis of ankle impingement than FSE-T2WI and FSE-PDWI. In summary, arthroscopy surgery for ankle impingement syndrome has several advantages such as good efficacy, minimal trauma, quick recovery, and much less complications. The preoperative FS-3D-FISP MRI allows accurate diagnosis and positioning of ankle impingement syndrome.
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http://dx.doi.org/10.1007/s00590-012-1078-9DOI Listing
October 2013

Robust high-speed binocular 3D eye movement tracking system using a two-radii eye model.

Conf Proc IEEE Eng Med Biol Soc 2006;2006:5302-6

Department of Computer & Information Science, Brooklyn College of City University of New York, Brooklyn, NY 11210, USA.

Existing video-based eye movement tracking systems measure three-dimensional eye orientations by assuming the eye is a sphere that rotates around its center at a fixed radius. We found this model inaccurate. We have developed a system that uses a two-radii eye model, which assumes that the eye rotates around two different centers with different radii horizontally and vertically. We found this two-radii model more accurate in estimating the three-dimensional eye positions than the traditional one-radius eye model.
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http://dx.doi.org/10.1109/IEMBS.2006.259359DOI Listing
March 2008

Robust and real-time torsional eye position calculation using a template-matching technique.

Comput Methods Programs Biomed 2004 Jun;74(3):201-9

Department of Computer and Information Science, Brooklyn College of the City University of New York, 2900 Bedford Avenue, Brooklyn, NY 11210, USA.

Computation of eye rotation about the line of sight (torsion) using image processing techniques has traditionally used cross-correlation of iral signatures sampled from circular arcs centered on the pupil. We have developed a new algorithm that utilizes a template-matching technique to calculate torsional eye position. Iral signatures are obtained from two annuli centered on the pupil center. By assuming that torsional rotation of the eye is constrained between successive video frames (<2 degrees), only a small window of the previous reference signature is necessary to determine relative torsional eye displacement. This dramatically reduces the number of pixels needed for computing torsion. This algorithm is considerably faster, attains a higher accuracy, and exhibits considerably less noise than the cross-correlation technique. Running on a 800 MHz Intel-based Dual Processor Pentium III, with a Matrox frame grabber, the system is capable of processing three-dimensional eye position at a rate of 120 frames/s.
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http://dx.doi.org/10.1016/S0169-2607(03)00095-6DOI Listing
June 2004