Publications by authors named "Daniel Freire de Sousa"

3 Publications

  • Page 1 of 1

Comparison of early cardiovascular risk among Brazilian and African university students.

Clin Biochem 2020 Jan 1;75:7-14. Epub 2019 Nov 1.

Department of Clinical and Toxicological Analysis, Federal University of Ceará, Fortaleza, Ceará, Brazil.

Cardiovascular diseases are among the main causes of mortality worldwide, and dyslipidemia is a principal factor risk. Hence the study of biochemical markers is necessary for early diagnosis.

Objectives: Evaluate biomarkers to diagnose the risks of cardiovascular diseases in healthy Brazilian and African young adults.

Design & Methods: Weight, height, waist circumference, percentage of body fat and systemic blood pressure were measured; and fasting blood samples were taken for biochemical analysis. Triglycerides, total cholesterol, HDL-c, and apolipoproteins A-I and B were measured on automated equipment using commercially available kits, in addition to the tests of antioxidant capacity of HDL and the enzymatic activity of Paraoxonase 1.

Results: After statistical analysis, it was found that BMI, WC, fat (%), triglycerides, ApoB/ApoA-I ratio and Vmax were higher in Brazilians, while HDL-c, ApoA-I, Lag Time, Vmax and PON1 activity were higher in Africans. In Brazilians, the ApoB/ApoA-I ratio was related to obesity factors and lipid profile, but in Africans it was related only to lipids. The antioxidant capacity of HDL and PON1 activity was better in Africans. Through independence testing, we observed an association with moderate risk of myocardial infarction with gender in Africans. In the binary logistic regression analysis, it was found that men in general - and particularly African men - have higher risk of myocardial infarction than women; Odds Ratio 2144 (CI: 1343-3424) and 2281 (CI: 1082-4811), respectively.

Conclusions: The anthropometric and biochemical parameters of Brazilians, especially men, predispose them to greater risks of cardiovascular diseases.
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January 2020

The renal effects and initial characterization of venom from Steindachner, 1870.

Toxicol Rep 2014 2;1:812-819. Epub 2014 Oct 2.

Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil.

The venom of the snake is a mixture of proteins and toxic peptides with several important local and systemic actions, which are similar to those occurring in snake bites. The mechanisms involved in the local and systemic actions of this venom are unknown. The aims of the work were to initial characterization of venom and investigate the effects of the poison in the renal perfusion system and in cultured renal tubular cells of the type MDCK (Madin-Darby canine kidney). The venom is composed majority of proteins (86.3%) and this poison promoted changes in all the evaluated renal parameters, mainly decreasing renal perfusion pressure (PP) and renal vascular resistance (RVR) and increasing urine flow (UF) and glomerular filtration rate (GFR). The most relevant result was that this venom was highly detrimental to the renal tubules independent of the PP reduction, which was shown by a decrease in sodium (Na), potassium (K) and chloride (Cl) electrolyte transport in the studied concentrations. The glomeruli and tubules contain protein bodies and blood extravasation, which were observed by histological analysis. The venom of reduced viability of the MDCK cells only at high concentrations (50 and 100 μg/mL) with an IC of 169.5 μg/mL.
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October 2014

The renal effects of alginates isolated from brown seaweed Sargassum vulgare.

J Appl Toxicol 2008 Apr;28(3):364-9

Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Universidade Federal do Ceará, P.O. Box-3157, 60430-270, Fortaleza, Ceará, Brasil.

Alginates isolated from Sargassum vulgare, present a strong antitumor activity, associated with kidney reversible damage, as analysed by histopathology of treated animals. In the present study, the renal alteration mechanisms of S. vulgare alginates were investigated using the isolated perfused rat kidney and the isolated perfused rat mesenteric blood vessel methods. The results showed that the effects of Sargassum vulgare low viscosity (SVLV) alginate were more potent than those of Sargassum vulgare high viscosity (SVHV) alginate in the isolated rat kidney. The SVLV alginate caused considerable changes in renal physiology, as shown by an increase in parameters such as perfusion pressure, renal vascular resistance, glomerular filtration rate, urinary flow and sodium, potassium and chloride excretion and by reduction of chloride tubular transport. The effects of SVHV were weaker than those of SVLV. The effects of SVLV on kidney could be related to direct vascular action as demonstrated with SVLV alginate on mesenteric blood vessels. In conclusion, the Sargassum vulgare alginate altered the renal function parameters evaluated. S. vulgare low viscosity alginate renal effects were more potent than S. vulgare high viscosity alginate. It is suggested that physicochemical differences between SVHV and SVLV could explain the differences found in the results.
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April 2008