Publications by authors named "Daniel Araki Ribeiro"

212 Publications

The growing importance of cytogenetic biomonitoring studies in buccal mucosa of dental laboratory technicians.

Arch Environ Occup Health 2021 Nov 24:1-2. Epub 2021 Nov 24.

Departamento de Biociências, Universidade Federal de São Paulo, Baixada Santista, São Paulo, Brazil.

The manuscript emphasizes some issues for the correct understanding of the recent paper accepted for publication in the Archives of Environmental & Occupational Health titled "Determination of cytogenetic abnormalities in buccal mucosa of dental laboratory technicians" by Yuksel and Yildirim.
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http://dx.doi.org/10.1080/19338244.2021.2003646DOI Listing
November 2021

Cytogenetic Biomonitoring in Buccal Mucosa Cells of COVID-19 Patients: Preliminary Findings.

In Vivo 2021 Nov-Dec;35(6):3495-3499

Department of Biosciences, Institute of Health and Society, Universidade Federal de São Paulo (UNIFESP), Santos, SP, Brazil

Background/aim: COVID-19 may lead to progressive respiratory failure as a consequence of alveolar damage, resulting in death. The aim of this study was to evaluate cytogenetic damage in oral cells of COVID-19 patients by micronucleus assay.

Patients And Methods: A total of 11 COVID-19 patients aged 40.7±9.3 years (5 men and 6 women) were included in this study. For the control group, a total of 15 participants not infected with SARS-CoV-2 virus were included. The mean age was 41.6±6.2 years (5 men and 10 women).

Results: The results showed statistically significant differences (p<0.05) in micronucleated buccal mucosa cells of COVID-19 patients. In addittion, a statistically significant increase in karyolysis and karrhyorexis (p<0.05) was observed in COVID-19 patients compared to control.

Conclusion: SARS-CoV-2 virus can induce mutagenesis and cytotoxicity in oral cells.
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http://dx.doi.org/10.21873/invivo.12651DOI Listing
October 2021

Is micronucleus assay in oral exfoliated cells a suitable tool for biomonitoring children exposed to environmental pollutants? A systematic review.

Environ Sci Pollut Res Int 2021 Dec 8;28(46):65083-65093. Epub 2021 Oct 8.

Institute of Health and Society, Department of Biosciences, Federal University of São Paulo, UNIFESP, Rua Silva Jardim, 136, Room 332, Vila Mathias, São Paulo, Santos, SP, 11050-020, Brazil.

The aim of this review was to evaluate if micronucleus assay in oral exfoliated cells is a suitable tool for biomonitoring children exposed to environmental pollutants. Through the electronic databases PubMed/Medline, Scopus, and Web of Science, all published studies until April 2021 that examined the relationship between exposure to environmental pollutants and micronucleus frequency in oral cells were searched. All relevant articles using a combination of the following keywords-"children," "micronucleus," "oral cells," and "environmental pollution"-were considered. A total of 20 papers met the criteria for inclusion in the systematic review. The results regarding the cytogenetic damage induced by environmental pollutants are conflicting. Some authors have demonstrated that environmental pollution induces mutagenesis in oral cells while others did not. Following the parameters of the Project for Effective Public Health Practices (EPHPP) and after extensive reading of all the articles included, a total of 12 articles had moderate and strong scores and 8 had a classification considered weak. Taken together, this review was able to demonstrate the association between micronucleus frequency and exposure to environmental pollutants in oral exfoliated cells of children.
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http://dx.doi.org/10.1007/s11356-021-16810-1DOI Listing
December 2021

Is micronucleus assay suitable for biomonitoring of children undergoing tooth restoration? Some concepts and definitions.

Cent Eur J Public Health 2021 09;29(3):244

Department of Biosciences, Institute of Health and Society, Federal University of Sao Paulo - UNIFESP, Santos, Sao Paulo, Brazil.

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http://dx.doi.org/10.21101/cejph.a6340DOI Listing
September 2021

Articular cartilage degeneration and bone adaptation due to lack of dystrophin in mice.

J Bone Miner Metab 2021 Sep 22. Epub 2021 Sep 22.

Departamento de Biociências, Universidade Federal de São Paulo (UNIFESP), Rua Silva Jardim, 136 Lab 328, CEP: 11015-020, Santos, SP, 11060-001, Brazil.

Introduction: Duchenne muscular dystrophy is caused by the absence of dystrophin. This study aimed to investigate femoral morphological characteristics of lack of dystrophin in MDX mice, considering that this model, different from DMD patient, is not influenced by corticosteroids administration and limited ambulation.

Materials And Methods: Proximal femur of male 16-week-old Control and MDX mice were submitted to histological, morphometric (volume density of articular cartilage, compact bone, trabecular bone and bone marrow; articular cartilage layers area; articular cartilage cell area), and immunohistochemistry analysis for RUNX-2, RANK-L, MMP-2, MMP-9, Caspase-3 and KI-67.

Results: MDX showed loss of linearity of articular cartilage with subchondral bone transition and elevation of this subchondral bone to the articular surface when compared with control. In addition, MDX presented morphological difference in the pantographic network of collagen fibers. Volume density of trabecular bone tissue was higher in the MDX than Control, but volume density of articular cartilage was lower in MDX (p < 0.05). The articular cartilage layers and chondrocytes area were significantly smaller in MDX than Control. These results associated to MMPs and osteogenic markers of proximal femur revealed an adaptation process as a consequence of lack of dystrophin.

Conclusions: The morphological changes observed in the bone tissue of the MDX may be not only secondary to muscle weakness or chronic use of corticosteroids but also our results indicate connections between decrease of cartilage thickness, collagen network alteration and consequent subchondral changes that may lead to articular cartilage degeneration and bone adaptation mechanism in MDX mice.
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http://dx.doi.org/10.1007/s00774-021-01270-xDOI Listing
September 2021

Inflammatory activity and apoptosis are associated with tissue degeneration in the submandibular gland of rats submitted to paradoxical sleep deprivation.

Odontology 2021 Sep 18. Epub 2021 Sep 18.

Department of Biosciences, Institute of Health and Society, Federal University of São Paulo, UNIFESP, Rua Silva Jardim, 136, Room 332, Vila Mathias, Santos, SP, 11050-020, Brazil.

The aim of this study was to evaluate if paradoxical sleep deprivation is able to induce tissue degeneration, inflammatory activity and apoptosis in the submandibular gland of rats. A total of 24 male Wistar rats were distributed into the following groups: group 1-control (CTRL; n = 8): the animals were not submitted to any procedures; group 2-sleep deprivation (PS; n = 8): the animals were submitted to paradoxical sleep deprivation for 96 h and group 3-recovery (R; n = 8): the animals were submitted to sleep deprivation for 96 h, followed by a period of 96 h without any intervention. The following parameters were evaluated: histopathological analysis, immunohistochemistry for Ki-67, COX-2 and cleaved caspase-3 and gene expression of TNF-α, Interleukin 6 (IL-6), Interleukin 10 (IL-10) and cytochrome C by real-time PCR. The results pointed out cytoplasmic vacuoles and congested vessels in the parenchyma of submandibular gland the in PS and R groups. The expression of interleukins 6, 10 and TNF-ɑ was differentially expressed in the PS and R groups. Apoptosis was also triggered by means of increasing cleaved caspase-3 and cytochrome c expression. The cellular proliferation (Ki-67 index) was also positive in the R group. Taken together, our results demonstrate that sleep deprivation is capable of promoting tissue degeneration in the submandibular gland, as a result of inflammatory response and cellular death in rats.
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http://dx.doi.org/10.1007/s10266-021-00657-6DOI Listing
September 2021

Does Panoramic X-ray Induce Cytogenetic Damage to Oral Cells? A Systematic Review With Meta-analysis.

Anticancer Res 2021 Sep 1;41(9):4203-4210. Epub 2021 Sep 1.

Department of Biosciences, Institute of Health and Society, Federal University of São Paulo, UNIFESP, Santos, SP, Brazil;

Aim: The aim of this review was to evaluate the scientific literature regarding the cytogenetic damage in oral exfoliated cells of adult patients submitted to panoramic X-ray.

Materials And Methods: An extensive search of the literature was conducted on PubMed, Scopus and Web of Science databases for all studies published until April 2021 using combinations of the following keywords: "panoramic X-ray," "DNA damage," "genetic damage", "genotoxicity", "mutagenicity", cytotoxicity", "buccal cells", "oral mucosa", "tongue", "gingiva", "micronucleus assay", according to the PRISMA guidelines. All clinical studies in English language were included in the study. A total of 10 studies were identified.

Results: As expected, the results regarding the cytogenetic damage induced by panoramic X-ray are conflicting. Some authors have demonstrated that panoramic X-ray induces mutagenesis in oral cells, whereas others did not. After reviewing the 10 studies, two were classified as strong, four were considered moderate, and four were considered weak, according to the quality assessment components of the Effective Public Health Practice Project (EPHPP). Meta-analysis data revealed a negative response related to mutagenicity in oral cells by panoramic X-ray.

Conclusion: Taken together, this review failed to demonstrate the association between micronucleus frequency and panoramic X-ray.
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http://dx.doi.org/10.21873/anticanres.15224DOI Listing
September 2021

In vitro and in vivo biological performance of hydroxyapatite from fish waste.

J Mater Sci Mater Med 2021 Aug 28;32(9):109. Epub 2021 Aug 28.

Department of Biosciences, Federal University of São Paulo, UNIFESP, Santos, SP, Brazil.

The aim of this study was to evaluate biocompatibility of hydroxyapatite (HAP) from fish waste using in vitro and in vivo assays. Fish samples (whitemouth croaker - Micropogonias furnieri) from the biowaste was used as HAP source. Pre-osteoblastic MC3T3-E1 cells were used in vitro study. In addition, bone defects were artificially created in rat calvaria and filled with HAP in vivo. The results demonstrated that HAP reduced cytotoxicity in pre-osteoblast cells after 3 and 6 days following HAP exposure. DNA concentration was lower in the HAP group after 6 days. Quantitative RT-PCR did not show any significant differences (p > 0.05) between groups. In vivo study revealed that bone defects filled with HAP pointed out moderate chronic inflammatory cells with slight proliferation of blood vessels after 7 and 15 days. Chronic inflammatory infiltrate was absent after 30 days of HAP exposure. There was also a decrease in the amount of biomaterial, being followed by newly formed bone tissue. All experimental groups also demonstrated strong RUNX-2 immoexpression in the granulation tissue as well as in cells in close contact with biomaterial. The number of osteoblasts inside the defect area was lower in the HAP group when compared to control group after 7 days post-implantation. Similarly, the osteoblast surface as well as the percentage of bone surface was higher in control group when compared with HAP group after 7 days post-implantation. Taken together, HAP from fish waste is a promising possibility that should be explored more carefully by tissue-engineering or biotechnology.
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http://dx.doi.org/10.1007/s10856-021-06591-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8403112PMC
August 2021

Could parental high-fat intake program the reproductive health of male offspring? A review.

Crit Rev Food Sci Nutr 2021 Aug 26:1-8. Epub 2021 Aug 26.

Departamento de Biociências, Universidade Federal de São Paulo, Santos, São Paulo, Brazil.

High-fat diet (HFD) intake can cause overweight and obesity and has become a global public health concern in recent years. Nutritional adversity at vulnerable windows of development can affect developing cells and their functions, including germ cells. Evidence shows that parental HFD intake prior to conception and/or during gestation and lactation could program the reproductive health of male offspring, ultimately resulting in impairment of the first as well as subsequent generations. In male offspring, adipose tissue and hypothalamic-pituitary-gonadal axis imbalance can impair the production of gonadotropins, leading to dysfunction of testosterone production and pubertal onset. The gonads can be directly impaired through oxidative stress, causing poor testosterone production and spermatogenesis; low sperm count, viability, and motility; and abnormal sperm morphology, which results in low sperm quality. Parental HFD intake could also be a risk factor for prostate hyperplasia and cancer in advanced age. It can impact the reproductive pattern of male offspring resulting in impairments in the subsequent generations. The investigation of semen quality must be extended to epidemiological and clinical studies of the male offspring of overweight and/or obese parents in order to improve the quality of human semen. This review addresses the effects of parental HFD intake on the reproductive parameters of male offspring and discusses the possible underlying mechanisms.
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http://dx.doi.org/10.1080/10408398.2021.1970509DOI Listing
August 2021

Inhibition of Toll Like Signaling Pathway Is Associated With Genomic Instability in Rat Liver Exposed to Crack Cocaine.

In Vivo 2021 Sep-Oct;35(5):2641-2646

Department of Biosciences, Institute of Health and Society, Federal University of Sao Paulo, UNIFESP, Santos, SP, Brazil

Background/aim: The aim of the present study was to investigate the biological effects of subacute crack cocaine exposure in rat liver.

Material And Methods: A total of 32 rats were distributed into four groups (n=8): Experimental group 1 (G1) and Experimental group 2 (G2): rats received 18 mg/kg of body weight (b.w) of crack cocaine for 5 days, once a day, group G2 remained 72 h without exposure after the experimental period (5 days)(abstinence); Experimental group 3 (G3): rats received 36 mg/kg of body weight (b.w) of crack cocaine for 5 days, once a day; Control Group (CTRL): rats received only the vehicle (DMSO) administered by the intraperitoneal (i.p) route for 5 days, once a day.

Results: All groups exposed to crack cocaine had an increase in the number of micronucleated hepatocytes and binucleated cells only in the highest tested dose (36 mg/kg). Karyolysis had an increase in the 18 mg/kg dose, in the abstinence group (G2), and 36 mg/kg group (G3); whereas pyknotic nuclei had an increase in the G2 group. The group exposed to 18 mg/kg of crack cocaine also showed high 8 OHdG expression. The p-NF-κB p65 protein decreased in the groups exposed to crack cocaine at doses of 18 and 36 mg/kg, as well as in the abstinence group. MyD88 was also found decreased in the group exposed to crack cocaine at 18 mg/kg.

Conclusion: Crack cocaine inhibited toll like signaling pathway whilst being associated with genomic instability in rat liver cells.
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http://dx.doi.org/10.21873/invivo.12546DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8408735PMC
August 2021

Sub-lethal combined effects of illicit drug and decreased pH on marine mussels: A short-time exposure to crack cocaine in CO enrichment scenarios.

Mar Pollut Bull 2021 Oct 21;171:112735. Epub 2021 Jul 21.

Department of Ecotoxicology, Santa Cecília University (UNISANTA), Santos, SP, Brazil; Department of Marine Sciences, Federal University of São Paulo (UNIFESP), Santos, SP, Brazil.

The increasing CO-concentrations in the atmosphere promote ocean acidification. Seawater chemistry changes interact with contaminants, such as illicit drugs in the coastal zones. This work evaluates impacts of pH decrease and crack-cocaine exposure on the commercial mussel Perna perna through biomarker responses (lysosomal membrane stability, lipid peroxidation, and DNA strand breaks). The organisms were exposed to different crack-cocaine concentrations (0.5, 5.0, and 50 μg L) combined with different pH values (8.3, 8.0, 7.5, 7.0, 6.5, and 6.0) for 96 h. Crack-cocaine in the different acidification scenarios triggered cyto-genotoxicity, which affected the overall health of mussels exposed to cocaine environmentally relevant concentration. This study produced the first data on biomarker responses associated with CO-induced acidification and illicit drugs (crack-cocaine) in marine organisms.
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http://dx.doi.org/10.1016/j.marpolbul.2021.112735DOI Listing
October 2021

Carotenoids obtained from an ionic liquid-mediated process display anti-inflammatory response in the adipose tissue-liver axis.

Food Funct 2021 Sep 20;12(18):8478-8491. Epub 2021 Sep 20.

Department of Biosciences, Federal University of São Paulo (UNIFESP), Silva Jardim Street, 136, Vila Mathias, 11015-020, Santos, SP, Brazil.

Ionic liquids (ILs) have been proposed as more efficient and sustainable solvents to replace volatile organic solvents (VOSs). However, the drawbacks associated with their use are still limiting the regular application of bioactive compounds obtained from the processes they mediate as food ingredients. It is true that the number of ILs approved by the Food and Drug Administration for food applications is still low and mainly focused on the ones from the quaternary ammonium family. However, this trend is changing, judging from the evidence that industries are surpassing overgeneralization about ILs (on price and toxicity) and starting to consider the potential and performance of ILs as solvents. Despite the examples of industries applying ILs in their processes, the use of bioactive compounds obtained from IL-based processes as ingredients in food formulations is still a big challenge. The positive influence of carotenoids on diseases associated or originating from the inflammatory scenario including, among others, obesity, is not new. Moreover, it is also well known that the poorest population worldwide does not have the recommended intake of carotenoids, especially those pro-vitaminic A. In an attempt to help answer this issue, dietary supplements containing adequate doses of natural carotenoids are expected to be the solution, or at least, part of the solution for a healthier life, but also, to reduce hunger. Thus, complete studies evaluating the toxicological potential and the real viability of adding these bioactive compounds in food formulations proving (or not!) their safety to consumers and handlers are highly demanded. This work proposes to investigate the potential of carotenoids extracted from feedstocks mediated by an ethanolic solution of an imidazolium-based IL. Thus, male Wistar rats were randomized in six different groups, supplemented or not by carotenoids extracted by IL or VOS, and fed by control- and/or high-fat-diets (HFD). The adipose tissue-liver axis was studied as a model to investigate the influence of the carotenoids on the levels of inflammation and oxidative stress markers. The main results showed that animals supplemented with carotenoids extracted with IL displayed improvements in serum parameters, besides lower metabolic efficiency, and antioxidant response on the liver, even when fed with HFD. However, animals supplemented with carotenoids extracted by VOS showed higher levels of pro-inflammatory markers and huge oxidative stress on the liver.
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http://dx.doi.org/10.1039/d1fo01429cDOI Listing
September 2021

Resveratrol effects in oral cancer cells: a comprehensive review.

Med Oncol 2021 Jul 17;38(8):97. Epub 2021 Jul 17.

Department of Biosciences, Institute of Health and Society, Federal University of São Paulo, Rua Silva Jardim, UNIFESP, 136, Room 332, Vila Mathias, Santos, SP, 11050-020, Brazil.

Oral cancer is a very common tumor worldwide with high incidence and mortality. The treatment of oral cancer involves surgery, radio- and chemotherapy; however, high failure rates and toxicity are noticed. Thus, the search of new drugs aiming a more effective treatment is welcomed. Natural products present chemopreventive and anti-cancer effects. Resveratrol is a naturally occurring antioxidant that contains several health benefits, including anti-inflammatory and antiproliferative activities. This review discusses the different action mechanisms of resveratrol related in the in vitro and in vivo studies using models of oral cancer.
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http://dx.doi.org/10.1007/s12032-021-01548-0DOI Listing
July 2021

Dimethoate induces genotoxicity as a result of oxidative stress: in vivo and in vitro studies.

Environ Sci Pollut Res Int 2021 Aug 29;28(32):43274-43286. Epub 2021 Jun 29.

Institute of Heath and Society, Department of Biosciences, Federal University of São Paulo, UNIFESP, Rua Silva Jardim, 136, Room 332, Vila Mathias, Santos, SP, 11050-020, Brazil.

Dimethoate ([O,O-dimethyl S-(N-methylcarbamoylmethyl) phosphorodithioate]) is an organophosphate insecticide and acaricide widely used for agricultural purposes. Genotoxicity refers to the ability of a chemical agent interact directly to DNA or act indirectly leading to DNA damage by affecting spindle apparatus or enzymes involved in DNA replication, thereby causing mutations. Taking into consideration the importance of genotoxicity induced by dimethoate, the purpose of this manuscript was to provide a mini review regarding genotoxicity induced by dimethoate as a result of oxidative stress. The present study was conducted on studies available in MEDLINE, PUBMED, EMBASE, and Google scholar for all kind of articles (all publications published until May, 2020) using the following key words: dimethoate, omethoate, DNA damage, genetic damage, oxidative stress, genotoxicity, mutation, and mutagenicity. The results showed that many studies were published in the scientific literature; the approach was clearly demonstrated in multiple tissues and organs, but few papers were designed in humans. In summary, new studies within the field are important for better understanding the pathobiological events of genotoxicity on human cells, particularly to explain what cells and/or tissues are more sensitive to genotoxic insult induced by dimethoate.
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http://dx.doi.org/10.1007/s11356-021-15090-zDOI Listing
August 2021

The importance of biomonitoring studies for detecting cytogenetic damage in cervical biopsy samples infected with human papillomavirus type 16.

Diagn Cytopathol 2021 Sep 24;49(9):1070-1071. Epub 2021 Jun 24.

Department of Biosciences, Institute of Health and Society, Federal University of Sao Paulo, UNIFESP, Sao Paulo, Brazil.

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http://dx.doi.org/10.1002/dc.24814DOI Listing
September 2021

Association between occupational exposure to domestic solid waste and dental caries: a cross-sectional study.

Environ Sci Pollut Res Int 2021 Nov 23;28(43):60717-60725. Epub 2021 Jun 23.

Department of Oral Pathology, School of Dentistry, Federal University of Bahia, Salvador, Bahia, Brazil.

Studies have shown that domestic waste collectors are exposed to toxicants including infectious pathogens, which may be linked to their oral health conditions. This cross-sectional study evaluated the dental caries and its associated factors among domestic waste collectors. A total of 301 adult men who worked for a waste collection corporation were included; 171 men worked in direct contact with domestic solid waste and 130 did not. Sociodemographic data, working, and medical history were assessed. The decayed, missing, and filled permanent teeth (DMFT) index was examined. Logistic regression analysis was used to identify factors associated with dental status with a significance level of 5%. The overall mean DMFT score was 8.36±5.64. The mean DMFT, missing teeth (MT), and filled teeth (FT) were significantly higher in workers who did not have any direct contact with waste (p≤0.04). In the logistic analysis, DMFT ≥8 was only associated with older age (OR=8.41 [95% confidence interval (95%CI), 5.01-14.12], p<0.001). Decayed teeth (DT) ≥2 was associated with no previous oral hygiene instruction (OR=2.70 [1.50-4.81], p=0.001) and no daily dental flossing (OR=4.26 [1.92-9.43], p<0.001). MT ≥9 was associated with lower education level (OR=3.33 [1.57-7.10], p= 0.002). FT≥3 had a negative association with low income (OR= 0.42 [0.25-0.70], p<0.001) and no daily flossing (OR=0.42 [0.23-0.76], p=0.004). In conclusion, occupational exposure to domestic solid waste was not associated with poor dental status. Instead, age, education, income level, and oral hygiene were associated with dental health status. Missing teeth constituted the major component of the DMFT index. Therefore, prevention and oral rehabilitation programs are necessary to improve dental health.
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http://dx.doi.org/10.1007/s11356-021-14890-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8221742PMC
November 2021

experimental study to investigate cytogenotoxicity of a contaminated estuary from Southeastern Brazilian Coast.

J Environ Sci Health A Tox Hazard Subst Environ Eng 2021 21;56(5):525-536. Epub 2021 May 21.

Department of Biosciences, Federal University of Sao Paulo, UNIFESP, Santos, SP, Brazil.

The aim of this study was to evaluate cytogenotoxicity in mammalian cells induced by ingestion of superficial water from SESS. For this purpose, surface water was collected from two points of SESS: São Vicente Channel (SVC) and Piaçaguera Channel (PIC). Four groups (n = 5) of adult male Wistar (8 weeks old) received for 5 days: (a) filtered tap water (water control), (b) tap water with 2.4% of NaCl (saline control), (c) estuarine water from PIC and (d) estuarine water from SVC. Results demonstrated that Ki67 immunoexpression was higher in hepatocytes exposed to both sampling site, while demonstrated downregulation in rat liver exposed to estuarine water. There was also significant increase in micronuclei frequency in bone marrow cells and hepatocytes, and DNA damage in blood and liver of rats exposed to estuarine water from SVC and PIC. In summary, studies with complex mixtures, such as contaminated estuarine water are important since this work confirmed by experiments using mammalian cells of rats that SESS water are genotoxic, mutagenic and cytotoxic, denoting concern for environmental health.
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http://dx.doi.org/10.1080/10934529.2021.1892016DOI Listing
June 2021

Comment on "Micronucleus Evaluation in Exfoliated Human Buccal Epithelium Cells among E-Waste Workers in Payatas, the Philippines".

J Health Pollut 2021 Mar 25;11(29):210309. Epub 2021 Feb 25.

Department of Biosciences, Federal University of Sao Paulo-UNIFESP, Rua Silva Jardim, 136, Room 332, Vila Mathias, Santos-SP, 11050-020, Brazil, Tel. +55 1332290156

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http://dx.doi.org/10.5696/2156-9614-11.29.210309DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8009652PMC
March 2021

Sleep Deprivation Interferes with JAK/STAT Signaling Pathway and Myogenesis in the Masseter Muscle of Rats.

Med Princ Pract 2021 18;30(3):253-261. Epub 2021 Feb 18.

Department of Biosciences, Federal University of São Paulo, UNIFESP, Santos, Brazil.

Objectives: The aim of the study was to study the Janus kinase/tyrosine kinase-activated transduction factor (JAK/STAT) signaling pathway and myogenesis on the masseter muscle after sleep deprivation and to investigate the role of stress in this scenario.

Subjects And Methods: A total of 18 male Wistar rats were divided into the following groups: control (n = 6): animals were not submitted to any procedures, and paradoxical sleep deprivation and vehicle (PSD + V; n = 6): animals were subjected to PSD for 96 h and (PSD + MET; n = 6): animals were subjected to PSD for 96 h with administration of metyrapone. Paradoxical sleep deprivation was performed by the modified multiple platforms method. Histopathological analysis, histomorphometry, and immunohistochemistry were performed.

Results: The results showed the presence of inflammatory infiltrate in the PSD + V and PSD + MET groups and atrophy. Histomorphometry showed that the cellular profile area decreased, while cellular density increased in both experimental groups. Expression of p-STAT 3, MyoD, and MyoG increased in the PSD + V group, while the PSD + MET group showed increased expression of IL-6 and p-STAT 3.

Conclusion: Our results suggest that sleep deprivation induces an inflammatory response and atrophy in the masseter muscle of rats.
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http://dx.doi.org/10.1159/000515307DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8280453PMC
February 2021

Repeated crack cocaine administration alters panic-related responses and delta FosB immunoreactivity in panic-modulating brain regions.

Exp Brain Res 2021 Apr 10;239(4):1179-1191. Epub 2021 Feb 10.

Departamento de Biociências, Universidade Federal de São Paulo (UNIFESP), Santos, SP, Brazil.

Crack cocaine is the crystal form of cocaine, produced by adding sodium bicarbonate to cocaine base paste. Brazil is the largest consumer of crack cocaine in the world. Users of crack cocaine show important physiological and behavioral alterations, including neuropsychiatric symptoms, such as anxiety-related symptoms. Nevertheless, few pre-clinical studies have been previously performed to understand the neurobiological effects of crack cocaine. The purpose of the present study was to investigate effects of the subchronic treatment (5 days, IP) of rats with crack cocaine in an animal model of anxiety/panic, the elevated T-maze (ETM). The ETM model allows the measurement of two behavioral defensive responses, avoidance and escape, in clinical terms, respectively, associated to generalized anxiety and panic disorder, the two main psychiatric conditions that accompany substance use disorders. Immediately after the ETM model, animals were tested in an open field for locomotor activity assessment. Analysis of delta FosB protein immunoreactivity was used to map areas activated by crack cocaine exposure. Results showed that crack treatment selectively altered escape displayed by rats in the ETM test, inducing either a panicolytic (18 mg/kg IP) or a panicogenic-like effect (25 and 36 mg/kg IP). These effects were followed by the altered functioning of panic-modulating brain regions, i.e., the periaqueductal gray and the dorsal region and lateral wings of the dorsal raphe nucleus. Treatment with 36 mg/kg of crack cocaine also increased locomotor activity. These are the first observations performed with crack cocaine in a rodent model of anxiety/panic and contribute to a better understanding of the behavioral and neurobiological effects of crack cocaine.
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http://dx.doi.org/10.1007/s00221-020-06031-2DOI Listing
April 2021

Genotoxicity, oxidative stress, and inflammatory response induced by crack-cocaine: relevance to carcinogenesis.

Environ Sci Pollut Res Int 2021 Mar 3;28(12):14285-14292. Epub 2021 Feb 3.

Department of Biosciences, Federal University of São Paulo, UNIFESP, Rua Silva Jardim, 136, Room 332, Vila Mathias, Santos, SP, 11050-020, Brazil.

Crack-cocaine is a cocaine by-product widely consumed by general population in developing countries. The drug is low cost and is associated with more intense effects when compared to other illicit drugs. Genotoxicity, oxidative stress, and inflammatory response are considered crucial events in carcinogenesis, since they actively participate in the multistep process. The purpose of this paper was to provide a mini review regarding the relationship between carcinogenesis and genotoxicity, oxidative stress, and inflammation induced by crack-cocaine. The present study was conducted on search of the scientific literature from the published studies available in PubMed, MEDLINE, Scopus, and Google Scholar for all kind of articles (all publications to November 2020) using the following key words: crack-cocaine, DNA damage, genotoxicity, cellular death, cytotoxicity, mutation, oxidative stress, inflammation, and mutagenicity. The results showed that published papers available were almost all in vivo test system being conducted in humans or rodents. Crack-cocaine was able to induce genotoxicity and oxidative stress in mammalian cells. However, the role of inflammatory response after exposure to crack-cocaine was not conclusive so far. In summary, this study is consistent with the notion that crack-cocaine is a chemical carcinogen as a result of genotoxicity and oxidative stress induced in mammalian and non-mammalian cells.
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http://dx.doi.org/10.1007/s11356-021-12617-2DOI Listing
March 2021

Histopathological changes and oxidative damage in type I and type II muscle fibers in rats undergoing paradoxical sleep deprivation.

Cell Signal 2021 05 30;81:109939. Epub 2021 Jan 30.

Department of Biosciences, Post Graduate Program of Interdisciplinary Health Sciences, Universidade Federal de São Paulo, Santos, Brazil. Electronic address:

Background: previous studies have shown that muscle atrophy is observed after sleep deprivation (SD) protocols; however, the mechanisms responsible are not fully understood. Muscle trophism can be modulated by several factors, including energy balance (positive or negative), nutritional status, oxidative stress, the level of physical activity, and disuse. The metabolic differences that exist in different types of muscle fiber may also be the result of different adaptive responses. To better understand these mechanisms, we evaluated markers of oxidative damage and histopathological changes in different types of muscle fibers in sleep-deprived rats.

Methods: Twenty male Wistar EPM-1 rats were randomly allocated in two groups: a control group (CTL group; n = 10) and a sleep deprived group (SD group; n = 10). The SD group was submitted to continuous paradoxical SD for 96  h; the soleus (type I fibers) and plantar (type II fiber) muscles were analyzed for histopathological changes, trophism, lysosomal activity, and oxidative damage. Oxidative damage was assessed by lipid peroxidation and nuclear labeling of 8-OHdG.

Results: The data demonstrated that SD increased the nuclear labeling of 8-OHdG and induced histopathological changes in both muscles, being more evident in the soleus muscle. In the type I fibers there was signs of tissue degeneration, inflammatory infiltrate and tissue edema. Muscle atrophy was observed in both muscles. The concentration of malondialdehyde, and cathepsin L activity only increased in type I fibers after SD.

Conclusion: These data indicate that the histopathological changes observed after 96 h of SD in the skeletal muscle occur by different processes, according to the type of muscle fiber, with muscles predominantly composed of type I fibers undergoing greater oxidative damage and catabolic activity, as evidenced by a larger increase in 8-OHdG labeling, lipid peroxidation, and lysosomal activity.
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http://dx.doi.org/10.1016/j.cellsig.2021.109939DOI Listing
May 2021

The importance of genotoxicity studies for biomonitoring children exposed to X-ray.

Eur J Radiol 2021 Feb 11;135:109454. Epub 2020 Dec 11.

Department of Biosciences, Federal University of Sao Paulo, UNIFESP, SP, Brazil. Electronic address:

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http://dx.doi.org/10.1016/j.ejrad.2020.109454DOI Listing
February 2021

COVID-19 in age-related neurodegenerative diseases: is there a role for vitamin D3 as a possible therapeutic strategy?

Rev Neurosci 2021 02 12;32(2):235-247. Epub 2020 Nov 12.

Department of Physiology and Pharmacology, Federal University of Ceará (UFC), Fortaleza60430-275, Ceará, Brazil.

The coronavirus disease (COVID-19), identified in Wuhan, China, on December 2019, was declared a pandemic by the World Health Organization, on March, 2020. Since then, efforts have been gathered to describe its clinical course and to determine preventive measures and treatment strategies. Adults older than 65 years of age are more susceptible to serious clinical symptoms and present higher mortality rates. Angiotensin-converting enzyme 2 (ACE2) is a major receptor for some coronavirus infection, including SARS-COV-2, but is also a crucial determinant in anti-inflammation processes during the renin-angiotensin system (RAS) functioning - converting angiotensin II to angiotensin 1-7. The decline in ACE2 expression that occurs with aging has been associated to the higher morbidity and mortality rates in older adults. These observations highlight the importance of investigating the association between COVID-19 and age-related neurodegenerative disorders, i.e., Parkinson's and Alzheimer's diseases. A possible option to reduce the risk of COVID-19 is vitamin D supplementation, due to its anti-inflammatory and immune-system-modulating effects. It has also been suggested that vitamin D supplementation plays a role in slowing progression of Parkinson and Alzheimer. The present study is a literature review of articles published on the theme COVID-19, Parkinson and Alzheimer's diseases, and the role played by vitamin D. PUBMED, MEDLINE, and EMBASE databases were consulted. Results confirm neurodegenerative and neuroinflammatory effects of COVID-19, aggravated in Parkinson's and Alzheimer's patients, and the important role of vitamin D as a possible therapeutic strategy. Nevertheless, randomized controlled trials and large population studies are still warranted.
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http://dx.doi.org/10.1515/revneuro-2020-0074DOI Listing
February 2021

Letter to the Editor.

Biotech Histochem 2020 11;95(8):563-564

Department of Biosciences, Federal University of Sao Paulo, UNIFESP , Sao Paulo, Brazil.

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http://dx.doi.org/10.1080/10520295.2020.1821092DOI Listing
November 2020

Comments on "Monitoring human genotoxicity risk associated to urban and industrial Buenos Aires air pollution exposure" by Los A Gutiérrez et al. (2020).

Environ Sci Pollut Res Int 2021 01 29;28(4):4881-4882. Epub 2020 Aug 29.

Departments of Biosciences, Federal University of São Paulo - UNIFESP, Rua Silva Jardim, 136, Room 332, Vila Mathias, Santos, SP, 11050-020, Brazil.

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http://dx.doi.org/10.1007/s11356-020-10630-5DOI Listing
January 2021

Biomonitoring of workers occupationally exposed to hazardous condition: the importance of genotoxicity studies.

Int J Pharm Pract 2020 10 26;28(5):546-547. Epub 2020 Aug 26.

Department of Biosciences, Federal University of São Paulo, UNIFESP, Santos, SP, Brazil.

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http://dx.doi.org/10.1111/ijpp.12664DOI Listing
October 2020

Protective Effect of Grape and Apple Juices against Cadmium Intoxication in the Kidney of Rats.

Drug Res (Stuttg) 2020 Nov 20;70(11):503-511. Epub 2020 Aug 20.

Department of Biosciences, Federal University of São Paulo, UNIFESP, SP, Brazil.

Background: The objective of this study was to evaluate protective effect of grape and apple juices against toxicity induced by cadmium in the kidney of rats.

Methods: A total of 20 male-Wistar rats were distributed into four groups (n=5): Control group: animals received an intraperitoneal (i.p.) injection of 0.9% saline solution and after 15 days, 1 mL of water was administered for 15 days, via gavage; Cadmium group: animals received an intraperitoneal injection of cadmium chloride (1.2 mg/kg) and after 15 days, 1 mL of water was administered for 15 days via gavage; Cadmium+Grape Juice: animals received an i.p. injection of cadmium chloride (1.2 mg/kg), and after 15 days, 0.8 mL of grape juice was administered for 15 days, via gavage; Cadmium+Apple Juice: animals received i.p. injection of cadmium chloride (1.2 mg/kg) and after 15 days, 1.0 mL of apple juice was administered for 15 days, via gavage.

Results: Histopathological analysis revealed severe tubular lesion and necrosis in the group exposed to cadmium, while animals exposed to grape or apple juices showed a significant reduction of tissue injury. 8-OHdG immunoexpression, DNA damage, cytochrome C and catalase gene expressions and Toll like signaling pathway (TLR2, and pIKKα/β) decreased in animals treated with grape juice when compared to cadmium group.

Conclusion: Taken together, we conclude that grape and apple juices had a protective effect by means of antioxidant, antigenotoxic actions and for promoting tissue regeneration in the kidney of rats following cadmium intoxication.
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http://dx.doi.org/10.1055/a-1221-4733DOI Listing
November 2020

Cytogenetic Biomonitoring on Oral Mucosa Cells of Hookah Users: Is It Possible?

Asian Pac J Cancer Prev 2020 07 27;21(7):1849. Epub 2020 Jul 27.

Department of Biosciences, Federal University of Sao Paulo, UNIFESP, SP, Brazil.

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http://dx.doi.org/10.31557/APJCP.2020.21.7.1849DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7573397PMC
July 2020
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