Publications by authors named "Dandan Zhao"

272 Publications

Effects of COVID-19 vaccination status, vaccine type, and vaccination interval on IVF pregnancy outcomes in infertile couples.

J Assist Reprod Genet 2022 Jun 27. Epub 2022 Jun 27.

Center of Reproductive Medicine, Shengjing Hospital of China Medical University, No. 39 Huaxiang Road, Tiexi District, Shenyang, 110072, Liaoning Province, China.

Purpose: This study aimed to explore whether the coronavirus disease (COVID-19) vaccination of both partners in infertile couples, different types of COVID-19 vaccines, and the interval between complete vaccination and oocyte retrieval or embryo transfer (ET) affect the quality of embryos and pregnancy rates in in vitro fertilization (IVF).

Methods: This was a prospective cohort study, comprising 735 infertile couples conducted between December 6, 2021, and March 31, 2022, in a single university hospital-based IVF center. The patients were divided into different groups according to the vaccination status of both partners in infertile couples, type of vaccine, and interval between complete vaccination and IVF treatment. The embryo quality and pregnancy rates were compared among different groups.

Results: The results showed that embryo quality and pregnancy rates had no significant differences among different groups. The multivariate regression model showed that the vaccination status of both infertile couples, types of vaccines, and intervals had no significant effects on the clinical pregnancy rate.

Conclusions: The vaccination status of both partners in infertile couples, different types of vaccines, and time intervals have no effect on embryo quality and pregnancy rates in IVF. This is the first study to compare the vaccination status of both partners in infertile couples and the impact of different vaccine types on pregnancy rates and embryo quality in detail. Our findings provide evidence of vaccine safety for infertile couples wishing to undergo IVF treatment. This evidence is crucial for decision-making by clinicians and policymakers involved in IVF cycles.
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http://dx.doi.org/10.1007/s10815-022-02543-8DOI Listing
June 2022

A novel star-shaped trinuclear platinum(II) complex based on a 1,3,5-triazine core displaying potent antiproliferative activity against TNBC by the mitochondrial injury and DNA damage mechanism.

Dalton Trans 2022 Jun 22. Epub 2022 Jun 22.

Key Laboratory of Optoelectronic Chemical Materials and Devices of Ministry of Education, School of Optoelectronic Materials and Technologies, Jianghan University, Wuhan 430056, P. R. China.

Polynuclear platinum(II) complexes represent a class of great prospective Pt-based antitumor drugs that may expand the antitumor spectrum and overcome the clinical problems of drug resistance and side effects of platinum-based drugs. Herein, a novel star-shaped trinuclear platinum(II) complex [Pt(L-3H)Cl] (1, L = 2,4,6-tris[(2-hydroxybenzyl)(2-pyridylmethyl)amine]-1,3,5-triazine) and its monomer [Pt(L'-H)Cl] (2, L' = (2-hydroxybenzyl)(2-pyridylmethyl)amine) were synthesized and characterized. The antiproliferative activities of complexes 1 and 2 against a panel of human cancer cell lines including MDA-MB-231 (triple-negative breast cancer, TNBC), MCF-7 (breast), HepG-2 (liver), and A549 (lung) were investigated. The results revealed that 1 exhibited much higher antiproliferative properties than its monomer 2 against the tested cell lines. Importantly, 1 possessed 3.3-fold higher antiproliferative activity as compared with cisplatin against the TNBC cell line MDA-MB-231. Another TNBC cell line MDA-MB-468 is also sensitive to 1. The results indicated that 1 might have the potential to act as a candidate for the treatment of TNBC. Cellular uptake and distribution studies showed that 1 could pass through the membrane of cells and enter into cells and mainly accumulate in the nuclei and mitochondria. 1 could bind to DNA in a cooperative groove-electrostatic-platinating binding mode and induce stronger DNA double-strand breaks (DSBs) and damaging effects on MDA-MB-231 than cisplatin (upregulation of γ-H2AX). Moreover, the DNA damage could not be easily repaired (upregulation of p53), which would exert a much positive influence on the overcoming of drug resistance. Additionally, flow cytometry studies showed that 1 arrested the cell cycle in the G0/G1 phase, induced mitochondrial membrane depolarization, increased ROS generation, and induced cell apoptosis. The results demonstrated that 1 could target simultaneously mitochondria and nuclei that gave rise to mitochondrial injury and DNA damage and ultimately efficiently promote the apoptotic death of tumor cells. Further mechanistic studies showed that 1 induced MDA-MB-231 cell apoptosis the p53-mediated mitochondrial pathway by upregulating Bax and cytochrome and downregulating Bcl-2 proteins, leading to the activation of caspase-3 and upregulation of the cleaved-PARP level. Taken together, 1 with such a synergic mechanism has great potential to be an effective anticancer agent that can overcome treatment resistance in TNBC.
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http://dx.doi.org/10.1039/d2dt00895eDOI Listing
June 2022

High-Quality Genome Assembly of subsp. Provides Insights Into Its Resistance to Fungal Diseases in the Summer Rain Belt in East Asia.

Front Plant Sci 2022 17;13:879822. Epub 2022 May 17.

Key Laboratory of Silviculture of the State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.

The olive tree ( L.) is the most iconic fruit crop of the Mediterranean Basin. Since the plant was introduced to China in the 1960s, the summer rain climate makes it susceptible to pathogens, leading to some olive diseases. L. subsp. is natively distributed in the Yunnan province of China. It has a smaller fruit size, lower oil content, and higher resistance compared to subsp. , which makes subsp. a critical germplasm resource to be investigated. Here, a high-quality genome of subsp. with 1.38 Gb in size was assembled and anchored onto 23 pseudochromosomes with a mounting rate of 85.57%. It represents 96.6% completeness [benchmarking universal single-copy orthologs (BUSCO)] with a contig N50 of 14.72 Mb and a scaffold N50 of 52.68 Mb, which shows a significant improvement compared with other olive genomes assembled. The evaluation of the genome assembly showed that 92.31% of resequencing reads and an average of 96.52% of assembled transcripts could be aligned to the assembled genome. We found that a positively selected gene, , was shared with the results of transcriptome analysis. This gene belongs to the susceptible gene and negatively regulates the disease resistance process. Furthermore, we identified the genus which causes the leaf spot disease in the infected leaves. The high-quality chromosome-level genomic information presented here may facilitate the conservation and utilization of germplasm resources of this subspecies and provide an essential genetic basis for further research into the differences in oil content and resistance between subsp. and .
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http://dx.doi.org/10.3389/fpls.2022.879822DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9152427PMC
May 2022

Establishment of an induced pluripotent stem cell (iPSC) line (INNDSUi001-A) from a healthy female Chinese Han.

Stem Cell Res 2022 Jul 23;62:102819. Epub 2022 May 23.

Research Institute of Neuromuscular and Neurodegenerative Diseases and Department of Neurology, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan 250012, Shandong, People's Republic of China. Electronic address:

We have established the human induced pluripotent stem cell (hiPSC) line (INNDSUi001-A) from skin fibroblasts of a healthy 24-year-old female individual by using non-integrating reprogramming method. The resulted cells had typical features of embryonic stem cell as indicated by expression of specific pluripotency markers and had the capability of in vitro differentiation into the three germ layers. This iPSC line can be used as a healthy control for disease study.
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http://dx.doi.org/10.1016/j.scr.2022.102819DOI Listing
July 2022

A Bivalent COVID-19 Vaccine Based on Alpha and Beta Variants Elicits Potent and Broad Immune Responses in Mice against SARS-CoV-2 Variants.

Vaccines (Basel) 2022 Apr 29;10(5). Epub 2022 Apr 29.

Beijing Engineering Research Center of Protein and Antibody, Sinocelltech Ltd., Beijing 100176, China.

With the emergence and rapid spread of new pandemic variants, especially variants of concern (VOCs), the development of next-generation vaccines with broad-spectrum neutralizing activities is of great importance. In this study, SCTV01C, a clinical stage bivalent vaccine based on trimeric spike extracellular domain (S-ECD) of SARS-CoV-2 variants Alpha (B.1.1.7) and Beta (B.1.351) with a squalene-based oil-in-water adjuvant was evaluated in comparison to its two corresponding (Alpha and Beta) monovalent vaccines in mouse immunogenicity studies. The two monovalent vaccines induced potent neutralizing antibody responses against the antigen-matched variants, but drastic reductions in neutralizing antibody titers against antigen-mismatched variants were observed. In comparison, the bivalent vaccine SCTV01C induced relatively higher and broad-spectrum cross-neutralizing activities against various SARS-CoV-2 variants, including the D614G variant, VOCs (B.1.1.7, B.1.351, P.1, B.1.617.2, B.1.1.529), variants of interest (VOIs) (C.37, B.1.621), variants under monitoring (VUMs) (B.1.526, B.1.617.1, B.1.429, C.36.3) and other variants (B.1.618, 20I/484Q). All three vaccines elicited potent Th1-biased T-cell immune responses. These results provide direct evidence that variant-based multivalent vaccines could play important roles in addressing the critical issue of reduced protective efficacy against the existing and emerging SARS-CoV-2 variants.
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http://dx.doi.org/10.3390/vaccines10050702DOI Listing
April 2022

Retraction notice to "Luteolin retards CXCL12-induced Jurkat cells migration by disrupting transcription of CXCR4" [Experimental and Molecular Pathology 113 (2020) 104370].

Exp Mol Pathol 2022 06 23;126:104773. Epub 2022 May 23.

Department of Hematology, Jining No.1 People's Hospital, Jining, 272011, China.

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http://dx.doi.org/10.1016/j.yexmp.2022.104773DOI Listing
June 2022

Identification and Validation of Novel Biomarkers for Hepatocellular Carcinoma, Liver Fibrosis/Cirrhosis and Chronic Hepatitis B via Transcriptome Sequencing Technology.

J Hepatocell Carcinoma 2022 9;9:389-403. Epub 2022 May 9.

Department of Traditional and Western Medical Hepatology, Third Hospital of Hebei Medical University, Shijiazhuang, Hebei, People's Republic of China.

Purpose: The aim of this study was to identify and validate novel biomarkers for distinguishing among hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC), liver fibrosis/liver cirrhosis (LF/LC) and chronic hepatitis B (CHB).

Patients And Methods: Transcriptomic sequencing was conducted on the liver tissues of 5 patients with HCC, 5 patients with LF/LC, 5 patients with CHB, and 4 healthy controls. The expression levels of selected mRNAs and proteins were assessed by quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemical (IHC) staining, and were verified in validation set (n=200) and testing set (n=400) via enzyme-linked immunosorbent assay (ELISA).

Results: A total of 9 hub mRNAs were identified by short time-series expression miner and weighted gene co-expression network analysis. Of note, the results of qRT-PCR and IHC staining demonstrated that SHC adaptor protein 1 (SHC1), SLAM family member 8 (SLAMF8), and interleukin-32 (IL-32) exhibited gradually increasing trends in the four groups. Subsequent ELISA tests on the validation cohort indicated that the plasma levels of SHC1, SLAMF8 and IL-32 also gradually increased. Furthermore, a diagnostic model APFSSI (age, PLT, ferritin, SHC1, SLAMF8 and IL-32) was established to distinguish among CHB, LF/LC and HCC. The performance of APFSSI model for discriminating CHB from healthy subjects (AUC=0.966) was much greater compared to SHC1 (AUC=0.900), SLAMF8 (AUC=0.744) and IL-32 (AUC=0.821). When distinguishing LF/LC from CHB, APFSSI was the most outstanding diagnostic parameter (AUC=0.924), which was superior to SHC1, SLAMF8 and IL-32 (AUC=0.812, 0.684 and 0.741, respectively). Likewise, APFSSI model with the greatest AUC value displayed an excellent performance for differentiating between HCC and LF/LC than other variables (SHC1, SLAMF8 and IL-32) via ROC analysis. Finally, the results in the test set were consistent with those in the validation set.

Conclusion: SHC1, SLAMF8 and IL-32 can differentiate among patients with HCC, LF/LC, CHB and healthy controls. More importantly, the APFSSI model greatly improves the diagnostic accuracy of HBV-associated liver diseases.
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http://dx.doi.org/10.2147/JHC.S357380DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9112460PMC
May 2022

Green Space, Air Pollution, Weather, and Cognitive Function in Middle and Old Age in China.

Front Public Health 2022 2;10:871104. Epub 2022 May 2.

Department of Biostatistics, Shandong University, Jinan, China.

Prior research has shown that environmental hazards, such as limited green space, air pollution, and harmful weather, have the strong adverse impact on older adults' cognitive function; however, most of the studies were conducted in developed countries and limited to cross-sectional analyses. China has the largest aging population in the world so the research evidence from it can offer an insight to the study in other developing countries facing similar issues and inform future public health policy and disease control. This study examined the long-term impact of environmental factors, namely, green space coverage, air pollution, and weather conditions on cognitive function using a nationally representative sample consisting of adults aged 45 years and older selected from the China Health and Retirement Longitudinal Study (CHARLS 2011-2018), the China City Statistical Yearbook, and other sources. Multilevel growth curve models were utilized for analysis and the mediator effects of physical activity and social engagement on the relationship between environmental factors and cognitive function were examined. Findings of this study showed that after controlling for sociodemographic characteristics, annual precipitation of 80 cm or more, living in areas with July temperature of 28°C or higher, urban community, and green space coverage were positively associated with cognition score at the baseline and lower precipitation, urban community, and greater green space coverage were associated with slower cognitive decline over a 7-year period. The impact of gross domestic product (GDP) seemed to take into effect more and more over time. These effects did not substantially change after weekly total hours of physical activities and levels of social engagement were added. More research on the mechanisms of the effect of environmental factors on cognition is needed such as the subgroup analyses and/or with more aspects of environmental measures.
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http://dx.doi.org/10.3389/fpubh.2022.871104DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9108722PMC
May 2022

Clinicopathologic Profiles of Sporadic Late-Onset Nemaline Myopathy: Practical Importance of Anti-α-Actinin Immunostaining.

Neurol Neuroimmunol Neuroinflamm 2022 Jul 17;9(4). Epub 2022 May 17.

From the Department of Neurology (B.Z., C.Z., L.L., Y.S., Y.Z., C.Y.), Qilu Hospital (Qingdao), Cheeloo College of Medicine, Shandong University; Research Institute of Neuromuscular and Neurodegenerative Diseases and Department of Neurology (T.D., D.Z., F.L., C.Y.), Qilu Hospital, Shandong University; Department of Medicine Experimental Center (X.M.), Qilu Hospital (Qingdao), Cheeloo College of Medicine, Shandong University; Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry (Y.Y.), College of Life Sciences, Shanxi Normal University, Xi'an, China; Division of Neuropathology (J.-Q.L.), Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario, Canada; Mitochondrial Medicine Laboratory (C.Y.), Qilu Hospital (Qingdao); and Brain Science Research Institute (C.Y.), Shandong University, Jinan, China.

Background And Objectives: Sporadic late-onset nemaline myopathy (SLONM) is a treatable or otherwise fatal myopathy. Diagnosis of SLONM is still challenging, and no therapeutic consensus has been achieved. Here, we reported the clinicopathologic features and long-term follow-up data of SLONM in a Chinese cohort.

Methods: We performed a retrospective evaluation of clinical, pathologic, and treatment outcomes of 17 patients with SLONM diagnosed between March 1986 and April 2021 at our neuromuscular center. Immunohistochemistry (IHC) with antibodies against 5 Z-disc-associated proteins was performed in the muscle biopsies of SLONM to identify a potential pathologic marker in aid of diagnosis. In comparison, we also performed muscle IHC in patients with selective type II fiber atrophy (n = 22), neurogenic atrophy (n = 22), mitochondrial myopathy (n = 5), immune-mediated necrotizing myopathy (n = 5), and normal controls (n = 5).

Results: Most of the patients exhibited asymmetric limb muscles weakness (71%, 12/17) and neck extensor weakness (53%, 9/17). Immunofixation electrophoresis was performed in 11 patients, and 4 of them were identified with monoclonal gammopathy of undetermined significance (MGUS). EMG from 16 patients demonstrated a myopathic pattern with spontaneous activities in 69% (11/16) of them. Muscle MRI showed preferential involvement of paraspinal, gluteus minimus and medius, semimembranosus, and soleus muscles. Suspected nemaline bodies on modified Gomori trichrome were confirmed by IHC using anti-α-actinin antibody (100%, 17/17), anti-myotilin antibody (94%, 16/17), anti-desmin antibody (94%, 16/17), anti-α-B crystallin antibody (65%, 11/17), and anti-telethonin antibody (18%, 3/17) with various positive rates. Notably, anti-α-actinin IHC showed the highest percentage of strongly positive staining (77%, 13/17), being the only one without negative results. Moderate improvement following autologous stem cell transplantation (ASCT) was noted in 3/4 patients with MGUS; favorable outcomes were also achieved in 6/7 patients without MGUS, including 3 patients with complete recovery who were given a combined treatment of prednisone and another immunosuppressant.

Discussion: SLONM is a treatable myopathy with ASCT or traditional immunotherapy, especially when combined with steroids and immunosuppressants. Anti-α-actinin immunostaining is the most reliable pathologic marker to identify rod-bearing fibers, and it should be performed routinely in adult patients with undiagnosed nonnecrotic myopathies.
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http://dx.doi.org/10.1212/NXI.0000000000001184DOI Listing
July 2022

Translation and validation of the Chinese version of Patient-completed Asthma Knowledge Questionnaire and its implementation in patient education.

J Thorac Dis 2022 Apr;14(4):905-918

Department of Pulmonary and Critical Care Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Background: Poor control of asthma results from many factors, partly due to inadequate knowledge towards asthma among patients. It is necessary to know patients' knowledge level before education. However, there is no accepted instrument to evaluate knowledge of asthma in Chinese patients with asthma. The study aims to develop a Chinese version of Patient-completed Asthma Knowledge Questionnaire (PAKQ) to assess its reliability, validity, and responsiveness for testing its clinical application in Chinese adult patients with asthma.

Methods: After translation, back-translation, and cross-cultural adaptation of the PAKQ into Chinese version, a survey of patients with asthma (n=464) in China was conducted. Demographics and clinical data were collected in addition to questionnaires concerning cognition of asthma, education, history, and asthma control test score. The PAKQ was then completed. 14±4 days after the initial assessment, the participants completed the retested questionnaire and again completed the questionnaire immediately after education. The reliability and the construct validity were evaluated. The optimal cut-off points for predicting disease knowledge among asthma patients were determined using the Youden index method.

Results: The Chinese version of PAKQ showed high internal consistency (Cronbach's alpha =0.888) at baseline and an acceptable 2-week test-retest reliability (ICC =0.932, r=0.874). On the basis of large modification indices (>10), this four-factor questionnaire was found to fit the data satisfactorily (χ/df =1.695, RMSEA =0.039, GFI =0.856, CFI =0.885, and SRMR =0.058). Paired -tests showed significant changes on pre-educational and post-educational tests (t=22.83, df=463, P<0.0001). The optimal cut-off value of the PAKQ total score for assessing patients' knowledge level was 35 points (AUC =0.757).

Conclusions: The Chinese version of the PAKQ questionnaire was developed and validated in terms of reliability and validity as an effective instrument for the insight into asthma knowledge of adult patients with asthma in China. Future research will evaluate the utility of the instrument in clinical practice.
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http://dx.doi.org/10.21037/jtd-21-1604DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9096276PMC
April 2022

Long non-coding RNA MIR4435-2HG/microRNA-125a-5p axis is involved in myocardial ischemic injuries.

Bioengineered 2022 04;13(4):10707-10720

Department of Cardiology, The First Affiliated Hospital of China Medical University, Shenyang110001, Liaoning Province, China.

This study aimed to investigate whether and how long non-coding RNA (lncRNA) MIR4435-2 host gene (MIR4435-2HG) involved in acute myocardial ischemia/reperfusion (I/R). Blood samples were collected from acute myocardial infarction (AMI) patients to detect MIR4435-2HG expression. myocardial I/R mice model and HO-induced oxidative stress model were established. Echocardiography, TUNEL assay and lactate dehydrogenase (LDH) detection were performed to assess heart infarction and myocardium apoptosis. Relationship among microRNA-125a-5p (miR-125a-5p), MIR4435-2HG and Mitochondrial fission protein 1 (MTFP1) was predicted by Targetscan and verified by luciferase reporter assay. MIR4435-2HG was notably upregulated in AMI patients, myocardial I/R mice and HO-treated cells. Knockdown of MIR4435-2HG notably alleviated infraction volume, ejection fraction (EF) and fractional shortening (FS) levels, cell apoptosis portion and pro-apoptotic cleaved-caspase-3 and Cyt c expression caused by myocardial I/R and oxidative stress, as well as improved cardiomyocytes viability. Transfection with miR-125a-5p alleviated MIR4435-2HG-caused cardiomyocytes apoptosis during oxidative stress. MiR-125a-5p overexpression decreased luciferase activity of the wild-type MIR4435-2HG compared with the mutated MIR4435-2HG. The expression levels of MTFP1 were elevated in myocardium from MI mice model and HO-treated AC16 cardiomyocytes. In addition, miR-125a-5p overexpression inhibited MTFP1 expression, and could stimulate the wild-type MTFP1 promoter luciferase activity but not the mutated one. Our findings revealed the role of MIR4435-2HG in MI-induced myocardium injury and cardiomyocytes apoptosis, disclosed a novel MIR4435-2HG/miR-125a-5p regulatory axis during myocardial I/R, and thus identified a potential target for the therapy of myocardial IR injury.
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http://dx.doi.org/10.1080/21655979.2022.2051259DOI Listing
April 2022

Corrigendum: Propofol Alleviates DNA Damage Induced by Oxygen Glucose Deprivation and Reperfusion FoxO1 Nuclear Translocation in H9c2 Cells.

Front Physiol 2022 29;13:805972. Epub 2022 Mar 29.

Department of Internal and Emergency Medicine, Shanghai General Hospital, Shanghai Jiao Tong University, Shanghai, China.

[This corrects the article DOI: 10.3389/fphys.2019.00223.].
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http://dx.doi.org/10.3389/fphys.2022.805972DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9004669PMC
March 2022

Identification of potential plasma markers for hepatitis B virus-related chronic hepatitis and liver fibrosis/cirrhosis.

J Med Virol 2022 Aug 14;94(8):3900-3910. Epub 2022 Apr 14.

Department of Traditional and Western Medical Hepatology, Third Hospital of Hebei Medical University, Shijiazhuang, China.

The aim of this study was to identify potential plasma biomarkers for hepatitis B virus (HBV)-related liver diseases. High-throughput transcriptome sequencing analysis was performed on five patients with chronic hepatitis B (CHB), five patients with HBV-associated liver fibrosis/liver cirrhosis (LF/LC), and four healthy participants. By short time-series expression miner and functional analysis, aquaporin 1 (AQP1), dystroglycan 1 (DAG1), and hemoglobin subunit beta (HBB) were identified as potential biomarkers. Immunohistochemical analysis revealed that the expression levels of AQP1, DAG1, and HBB were upregulated in the three groups. Subsequent enzyme-linked immunosorbent assay tests on the training cohort (n = 150) indicated that the plasma levels of AQP1 and DAG1 were highest in LF/LC patients, followed by those in CHB patients, and the lowest in healthy controls. APAD model, a diagnostic panel incorporating age, platelet, AQP1, and DAG1 levels, exhibited the strongest stratification ability to distinguish LF/LC patients from CHB patients, and to differentiate CHB patients from healthy controls. Furthermore, the diagnostic accuracies of the biomarkers and APAD model were verified in an independent cohort consisting of 230 participants. In conclusion, both AQP1 and DAG1 have good diagnostic values and APAD model greatly enhances the diagnostic accuracy for HBV-related hepatic diseases.
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http://dx.doi.org/10.1002/jmv.27761DOI Listing
August 2022

MicroRNA networks in FLT3-ITD acute myeloid leukemia.

Proc Natl Acad Sci U S A 2022 04 11;119(16):e2112482119. Epub 2022 Apr 11.

Gehr Family Center for Leukemia Research, City of Hope Medical Center, Hematologic Malignancies Research Institute and Center for Stem Cell Transplantation, Duarte, CA 91010.

MiR-126 and miR-155 are key microRNAs (miRNAs) that regulate, respectively, hematopoietic cell quiescence and proliferation. Herein we showed that in acute myeloid leukemia (AML), the biogenesis of these two miRNAs is interconnected through a network of regulatory loops driven by the FMS-like tyrosine kinase 3-internal tandem duplication (FLT3-ITD). In fact, FLT3-ITD induces the expression of miR-155 through a noncanonical mechanism of miRNA biogenesis that implicates cytoplasmic Drosha ribonuclease III (DROSHA). In turn, miR-155 down-regulates SH2-containing inositol phosphatase 1 (SHIP1), thereby increasing phosphor-protein kinase B (AKT) that in turn serine-phosphorylates, stabilizes, and activates Sprouty related EVH1 domain containing 1 (SPRED1). Activated SPRED1 inhibits the RAN/XPO5 complex and blocks the nucleus-to-cytoplasm transport of pre-miR-126, which cannot then complete the last steps of biogenesis. The net result is aberrantly low levels of mature miR-126 that allow quiescent leukemia blasts to be recruited into the cell cycle and proliferate. Thus, miR-126 down-regulation in proliferating AML blasts is downstream of FLT3-ITD–dependent miR-155 expression that initiates a complex circuit of concatenated regulatory feedback (i.e., miR-126/SPRED1, miR-155/human dead-box protein 3 [DDX3X]) and feed-forward (i.e., miR-155/SHIP1/AKT/miR-126) regulatory loops that eventually converge into an output signal for leukemic growth.
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http://dx.doi.org/10.1073/pnas.2112482119DOI Listing
April 2022

Immunogenicity correlation in cynomolgus monkeys between Luminex-based total IgG immunoassay and pseudovirion-based neutralization assay for a 14-valent recombinant human papillomavirus vaccine.

J Med Virol 2022 Aug 21;94(8):3946-3955. Epub 2022 Apr 21.

Beijing Engineering Research Center of Protein and Antibody, Sinocelltech Ltd., Beijing, China.

A new virus-like particle based vaccine covering 14 types of high-risk and disease-inducing human papillomavirus (HPV) can offer better coverage against HPV-induced diseases, particularly cervical cancers. However, the assessment of immunogenicity of the vaccine is an important task, representing not only its significant clinical characteristics, but also a major challenge, in terms of both the suitability of methods and the clinical sample testing throughput supporting clinical development. This work covers the development and evaluation of a method based on Luminex technology (a coded-bead and flow-cytometric approach) to assess the HPV-type specific total immunoglobulin G (IgG). This method can evaluate the antibodies in sera post immunization against multiple types of HPV simultaneously (i.e., with multiplexing capability), save time and cost, and improve test throughput with higher sensitivity and precision than the classical, plate-based enzyme-linked immunoassay and competitive Luminex-based immunoassays. Using cynomolgus monkeys as model, we demonstrated the good correlation between the results from the pseudovirion-based neutralization assay (PBNA), and the Luminex-based total IgG assay, supporting that the latter method can be considered as a viable, dependable replacement method for the PBNA supporting immunogenicity evaluation of HPV vaccine in preclinical development and clinical investigation.
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http://dx.doi.org/10.1002/jmv.27763DOI Listing
August 2022

Nanopore-Based Single-Entity Electrochemistry for the Label-Free Monitoring of Single-Molecule Glycoprotein-Boronate Affinity Interaction and Its Sensing Application.

Anal Chem 2022 04 1;94(14):5715-5722. Epub 2022 Apr 1.

Anhui Key Laboratory of Chemo/Biosensing, College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, P. R. China.

Nanopipettes provide a promising confined space that enables advances in single-molecule analysis, and their unique conical tubular structure is also suitable for single-cell analysis. In this work, functionalized-nanopore-based single-entity electrochemistry (SEE) analysis tools were developed for the label-free monitoring of single-molecule glycoprotein-boronate affinity interaction for the first time, and immunoglobulin G (IgG, one of the important biomarkers for many diseases such as COVID-19 and cancers) was employed as the model glycoprotein. The principle of this method is based on a single glycoprotein molecule passing through 4-mercaptophenylboronic acid (4-MPBA)-modified nanopipettes under a bias voltage and in the meantime interacting with the boronate group from modified 4-MPBA. This translocation and affinity interaction process can generate distinguishable current blockade signals. Based on the statistical analysis of these signals, the equilibrium association constant (κ) of single-molecule glycoprotein-boronate affinity interaction was obtained. The results show that the κ of IgG in the confined nanopore at the single-molecule level is much larger than that measured in the open system at the ensemble level, which is possibly due to the enhanced multivalent synergistic binding in the restricted space. Moreover, the functionalized-nanopore-based SEE analysis tools were further applied for the label-free detection of IgG, and the results indicate that our method has potential application value for the detection of glycoproteins in real samples, which also paves way for the single-cell analysis of glycoproteins.
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http://dx.doi.org/10.1021/acs.analchem.2c00860DOI Listing
April 2022

B-Cell Lymphoma Associated with COVID 19 Infection: A Case Report.

Curr Med Imaging 2022 Mar 29. Epub 2022 Mar 29.

Department of Radiology, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310003, China.

Purpose: The most commen publications are related about COVID-19 diagnose in hematological malignancy patients, However, here we report a case involving a patient who was diagnosed with B-cell lymphoma while undergoing treatment for COVID-19, including the changes in major clinical symptoms and medical examinations, then explain of the probable causes of the case.

Case Presentation: A 74-year-old woman with a previous history of oesophageal cancer was admitted to the hospital after having cough and sputum for 15 days. Despite the COVID-19 symptoms, this patient did not have fever at time of the onset. Results of routine blood tests were normal at first but then declined with persistent fever, A whole-body CT examination ruled out the possibility of tumor-metastasis-related fever. This patient had no hepatosplenomegaly or regional lymphadenopathy, and there was no concrete evidence of haemophagocytic lymphohistiocytosis or lymphoma until bone marrow biopsy results confirmed the latter.

Conclusion: We describe an uncommen case of COVID-19 who was finally diagnosed with B-cell lymphoma. An awareness of persistent fever and declined routine blood tests caused by haematological malignancies instead of COVID-19 itself can aid in providing appropriate guidelines for management and treatment.
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http://dx.doi.org/10.2174/1573405618666220329210311DOI Listing
March 2022

Surface-Displayed ASFV (p14.5) Can Stimulate Immune Responses in Mice.

Vaccines (Basel) 2022 Feb 24;10(3). Epub 2022 Feb 24.

College of Veterinary Medicine, Jilin Agricultural University, Changchun 130118, China.

African Swine Fever Virus (ASFV) has spread worldwide, and the lack of vaccines severely negatively impacts the pig industry. In this study, the p14.5 protein encoded by ASFV was used as the antigen, and the p14.5 gene was expressed in vitro using the expression system. Three new functionally recombinant () were constructed and the expressions of the p14.5 protein, p14.5-IL-33-Mus fusion protein and CTA1-p14.5-D-D fusion protein were successfully detected using Western blot analysis. After oral immunization of SPF mice with recombinant , flow cytometry and ELISA were performed to detect the differentiation and maturity of T lymphocytes, B lymphocytes and DCs of the mice, which were higher than those of the control group. Specific antibodies were produced. The immunogenicity of the adjuvant group was stronger than that of the single antigen group, and the IL-33 adjuvant effect was stronger than that of the CTA1-DD adjuvant.
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http://dx.doi.org/10.3390/vaccines10030355DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8950097PMC
February 2022

Local and non-local drivers of consumption-based water use in China during 2007-2015: Perspective of metacoupling.

J Environ Manage 2022 Jun 21;312:114940. Epub 2022 Mar 21.

Laboratory for Earth Surface Processes, Ministry of Education, College of Urban and Environmental Sciences, Peking University, Beijing, 100871, China. Electronic address:

Increasingly growing consumption-based water use (WU) combined with climate change have exacerbated water stress globally and regionally, yet little is known about how the WU change is affected by metacoupled processes which involve human-nature interactions across space; within and across adjacent and distant places. This study aims to unveil the spatio-temporal pattern of China's WUs during 2007-2015 and its underlying local and non-local drivers. Results show that China's total WU exhibited an upward trend from 386.7 billion m³; in 2007 to 431.2 billion m³ in 2012 but dropped to 412.6 billion m³ by 2015. Widespread and continuous water use efficiency improvement contributed most to offsetting the increase in WU driven by the rising affluence and growing population in the context of rapid urbanization and industrialization. Economic structure drove a relatively large WU reduction (responsible for -23.7% of the WU change during 2007-2015), in line with China's ongoing transform from a capital investment-driven economy to a consumption-driven one and decoupling economic growth from environmental pressure. The population share representing the non-local factor of migration effect was large enough to be seen clearly in the changing WUs across China: the WUs of coastal areas ascended while inland areas descended, which was in accordance with migration patterns. Our findings could make a valuable contribution to decision-making in identifying hotspot areas, charting systematic courses for sustainable water use, and combining demand-oriented and supply-oriented measures.
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http://dx.doi.org/10.1016/j.jenvman.2022.114940DOI Listing
June 2022

LuQi Formula Ameliorates Myocardial Fibrosis by Suppressing TLR4/MyD88/NF-B Pathway and NLRP3 Inflammasome Activation in Mice with Myocardial Infarction.

Evid Based Complement Alternat Med 2022 11;2022:5867987. Epub 2022 Mar 11.

Institute of Cardiovascular Disease of Integrated Traditional Chinese and Western Medicine, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, China.

Background: Myocardial fibrosis caused by myocardial infarction (MI) is the key factor leading to cardiac remodeling; nod-like receptor family pyrin domain-containing 3 (NLRP3) plays an important role in regulation of myocardial injury; however, its relationship with TLR4/MyD88/NF-B signaling pathway is largely unreported. In recent years, traditional Chinese medicine (TCM) prevention and treatment of cardiovascular diseases has shown its unique advantages and broad application prospects. LuQi Formula (LQF) has been used for more than 20 years in Shuguang Hospital (Shanghai, China), and it was confirmed that it can improve the clinical symptoms of patients after MI. Here, we investigated the mechanism of LQF by suppressing NLRP3 inflammasome activation and TLR4/MyD88/NF-B pathway in mice with MI.

Purpose: The purpose of this study was to verify the positive effects of the LQF in ameliorating myocardial fibrosis and inflammasome infiltration in the MI mice in vivo.

Methods: Forty mice were randomized into four groups: the sham group, the MI group, the LQF group, and the perindopril group ( = 10 per group). Left anterior descending (LAD) coronary artery ligation was performed in all groups except the sham group. The mice were treated with LQF after MI. After 4 weeks, LDH, cTnI, IL-1, and IL-18 were measured by enzyme-linked immunosorbent assay (ELISA) kit, and cardiac function was evaluated by echocardiography. Hematoxylin and eosin (H&E) and Masson staining were used to evaluate the myocardial injury and fibrosis. Western blot was used to evaluate the expression of collagen I, -SMA, NLRP3 inflammasome, and TLR4/MyD88/NF-B signaling pathway. Immunohistochemical analysis was used to further detect the expression of Fibronectin, -SMA, collagen I, collagen III, NLRP3, and NF-B in myocardial tissue.

Results: Compared with the MI group, the ejection fraction (EF) and fractional shortening (FS) in the LQF group were significantly improved, while the left ventricular end diastolic diameter (LVEDd) and left ventricular internal dimension systole (LVIDs) were significantly decreased. The representative staining of H&E and Masson showed that treatment with LQF could effectively reduce myocardial injury and fibrosis. ELISA results showed that serum LDH, cTnI, TNF-, IL-18, and IL-1 in LQF group were significantly lower than those in MI group. The western blot results showed that the expressions of collagen I and -SMA were decreased significantly in the LQF group. Moreover, the expressions of NLRP3 inflammasome and TLR4/MyD88/NF-B signaling pathway were downregulated in the LQF treatment group.

Conclusion: Our results suggested that LQF could significantly improve cardiac function and ameliorate myocardial fibrosis. In addition, we found that LQF could downregulate the TLR4/MyD88/NF-B signaling pathway and then inhibit the activation of NLRP3 inflammasome, suggesting that LQF alleviated cardiac fibrosis by decreasing the TLR4/MyD88/NF-B signaling pathway and then inhibited NLRP3 inflammasome activation in MI mice, which indicates potential therapeutic effect of LQF on patients with MI.
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http://dx.doi.org/10.1155/2022/5867987DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8933100PMC
March 2022

Effect of copper on bioconcentration of benzotriazole ultraviolet stabilizers (BUVSs) in common carp (Cyprinus carpio).

Environ Res 2022 Aug 12;211:113121. Epub 2022 Mar 12.

Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Linggong Road 2, Dalian, 116024, China. Electronic address:

Benzotriazole ultraviolet stabilizers (BUVSs) have received increasing attention as emerging contaminants. However, most of the existing relevant studies focused on the adverse ecological effect of BUVSs under their single exposure, information about the bioconcentration potential of BUVSs and their joint exposure with heavy metals remains scarce. In this study, we investigated the bioconcentration kinetics of 6 frequently reported BUVSs in four main tissues of common carp under different Cu concentration. The bioconcentration factors (BCFs) and half-lives (t) in the fish tissues ranged from 5.73 (UV-PS in kidney) to 1076 (UV-327 in liver), and 2.19 (UV-PS in kidney) to 31.5 (UV-320 in liver) days, respectively. Under the effect of Cu, an increase in BCF values was observed, which is mainly due to the decreased depuration rate (k). These results indicated that BUVSs accumulated in fish and that Cu can affect the bioconcentration of BUVSs. This study provides important insight into the co-exposure of heavy metal and BUVSs, contributing to the perfection of BUVSs risk assessment.
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http://dx.doi.org/10.1016/j.envres.2022.113121DOI Listing
August 2022

How far are we from possible ideal virtual water transfer? Evidence from assessing vulnerability of global virtual water trade.

Sci Total Environ 2022 Jul 10;828:154493. Epub 2022 Mar 10.

Laboratory for Earth Surface Processes, Ministry of Education, College of Urban and Environmental Sciences, Peking University, Beijing 100871, China. Electronic address:

With the increasing contradiction between water demand and supply in a telecoupled society where nature and human interplay intensively over distance, virtual water trade (VWT) plays an indispensable role in global water use sustainability. There has been little quantitative analysis of global water use sustainability depicting both overall system characteristics and flows between subsystems. In such a context, the extent to which virtual water transfer deviates from possible ideal expectations (i.e., virtual water flows from water-abundant regions to water-scarce ones), and its impact on global water use sustainability, are not well evaluated. Therefore, the global VWT vulnerability framework is proposed to delineate the gap between the real VWT and the possible optimal scenario, providing potential space for future optimization and regulation. Represented by the ratio of weighted total virtual water volume to the original one, the vulnerability is assessed from 2005 to 2015 based on the Eora input-output database and Virtual Water Transfer Multiplier which assigns differentiated weights to per unit volume of virtual water transfer based on the water stress levels of importers and those of exporters. Results show that the global VWT vulnerability has increased by 18.9% during the study period, with Africa and Southern and Central Asia making the biggest contribution. Developed countries contributed around 80% of the increased global VWT vulnerability. However, the proportion has fallen a bit, in terms of the conventional view of developed countries taking the approach of transferring responsibility to developing countries. Instead, the proportions of transferring responsibility between developing countries have rose by 10% to 30% during 2005-2015, partially due to stronger trade ties among developing countries. Our findings support policy decisions on tracing environmental responsibility for water scarcity and call for action to prevent water ecological downsides due to international trade.
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http://dx.doi.org/10.1016/j.scitotenv.2022.154493DOI Listing
July 2022

Utilization of Hematite Particles for Economical Removal of -xylene in a High-Temperature Gas-Solid Reactor.

Molecules 2022 Feb 23;27(5). Epub 2022 Feb 23.

School of Environmental Science and Engineering, Hebei University of Science and Technology, Shijiazhuang 050018, China.

To establish a novel approach for VOCs resource utilization, coupled -xylene oxidation and hematite reduction was investigated in this study in a high-temperature gas-solid reactor in the temperature range 300-700 °C. As the -xylene-containing inert gas (N) stream traveled through the hematite particle bed, its reaction behavior was determined in programmed heating and constant temperature modes. Consequently, the effect of bed temperature, flow rate and -xylene inlet concentration on both -xylene removal performance and degree of hematite reduction was studied. The raw hematite and solid products were analyzed by TGA, XRF, XRD and SEM-EDS. The results showed that a temperature above 300 °C was required to completely eliminate -xylene by hematite, and both -xylene removal capacity and degree of hematite reduction at 5% breakthrough points enhanced on increasing the temperature and decreasing the flow rate. The increment in temperature from 300 °C to 700 °C led to a gradual reduction of FeO to FeO, FeO and metallic iron. Thus, this study provides a novel, economic and promising technology for treating the VOC pollutants.
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http://dx.doi.org/10.3390/molecules27051509DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8911638PMC
February 2022

Research Progress and Trends of Phenylethanoid Glycoside Delivery Systems.

Foods 2022 Mar 7;11(5). Epub 2022 Mar 7.

Institute of Agro-Production Storage and Processing, Xinjiang Academy of Agricultural Sciences, Ürümqi 830091, China.

Background: Phenylethanoid glycosides (PhGs) are obtained from a wide range of sources and show strong biological and pharmacological activities, such as antioxidant, antibacterial and neuroprotective effects. However, intestinal malabsorption and the low bioavailability of PhGs seriously affect their application. Delivery systems are an effective method to improve the bioavailability of active substances. Scope and approach: In this article, the biological activities of and delivery systems for PhGs are introduced. The application statuses of delivery systems for echinacoside, acteoside and salidroside are reviewed. Finally, the problems of the lack of uniform standards for delivery systems and the poor targeted delivery accuracy of PhGs in the current research are proposed and suggestions for future research are put forward based on those problems.

Key Findings And Conclusions: Although there are still some problems in the delivery system of phenylethanoside, such as inconsistent standards and inaccurate delivery, phenylethanoside itself has been proven to have a variety of physiological activities. Therefore, the action mechanism and application of phenylethanoside and its delivery system should be studied further.
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http://dx.doi.org/10.3390/foods11050769DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8909102PMC
March 2022

Lycopene Improves Bone Quality and Regulates AGE/RAGE/NF-кB Signaling Pathway in High-Fat Diet-Induced Obese Mice.

Oxid Med Cell Longev 2022 17;2022:3697067. Epub 2022 Feb 17.

Diabetes Research Center, Traditional Chinese Medicine School, Beijing University of Chinese Medicine, Beijing 100029, China.

Objective: This study was aimed at examining the effects of lycopene on bone metabolism in high-fat diet (HFD)- induced obese mice and to identify the potential underlying mechanisms.

Methods: Mice were fed a HFD for 12 weeks and then continue with or without lycopene intervention (15 mg/kg) for additional 10 weeks. The effects of lycopene on blood glucose and lipid metabolism, as well as serum levels of total antioxidant capacity (T-AOC), superoxide dismutase (SOD), and malondialdehyde (MDA) were determined by biochemical assays. Bone histomorphological features and osteoclast activity were assessed by hematoxylin/eosin and tartrate-resistant acid phosphatase staining. Bone microstructure at the proximal tibial metaphysis and diaphysis was determined by microcomputed tomography. Tibial biomechanical strength and material profiles were measured by a three-point bending assay and Fourier transform infrared spectroscopy. Protein expressions involved in the AGE/RAGE/NF-кB signaling pathway were determined by western blot and/or immunohistochemical staining.

Results: Lycopene consumption reduced body weight gain and improved blood glucose and lipid metabolism in HFD-induced obese mice. In addition, lycopene treatment preserved bone biomechanical strength, material profiles, and microarchitecture in obese mice. Moreover, these alterations were associated with an increase in serum levels of T-AOC and SOD, and a decline in serum levels of MDA, as well as a reduction of AGEs, RAGE, cathepsin K, and p-NF-кBp65 and NF-кBp65 expressions in the femurs and tibias of obese mice.

Conclusion: Lycopene may improve bone quality through its antioxidant properties, which may be linked with the regulation of the AGE/RAGE/NF-кB signaling pathway in obese mice. These results suggest that lycopene consumption may be beneficial for the management of obesity-induced osteoporosis.
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http://dx.doi.org/10.1155/2022/3697067DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8872668PMC
March 2022

African swine fever virus MGF360-11L negatively regulates cGAS-STING-mediated inhibition of type I interferon production.

Vet Res 2022 Jan 24;53(1). Epub 2022 Jan 24.

College of Veterinary Medicine, Jilin Agricultural University, Changchun, China.

The type I interferon (IFN-I) signaling pathway is an important part of the innate immune response and plays a vital role in controlling and eliminating pathogens. African swine fever virus (ASFV) encodes various proteins to evade the host's natural immunity. However, the molecular mechanism by which the ASFV-encoded proteins inhibit interferon production remains poorly understood. In the present study, ASFV MGF360-11L inhibited cGAS, STING, TBK1, IKKε, IRF7 and IRF3-5D mediated activation of the IFN-β and ISRE promoters, accompanied by decreases in IFN-β, ISG15 and ISG56 mRNA expression. ASFV MGF360-11L interacted with TBK1 and IRF7, degrading TBK1 and IRF7 through the cysteine, ubiquitin-proteasome and autophagy pathways. Moreover, ASFV MGF360-11L also inhibited the phosphorylation of TBK1 and IRF3 stimulated by cGAS-STING overexpression. Truncation mutation analysis revealed that aa 167-353 of ASFV MGF360-11L could inhibit cGAS-STING-mediated activation of the IFN-β and ISRE promoters. Finally, the results indicated that ASFV MGF360-11L plays a significant role in inhibiting IL-1β, IL-6 and IFN-β production in PAM cells (PAMs) infected with ASFV. In short, these results demonstrated that ASFV MGF360-11L was involved in regulating IFN-I expression by negatively regulating the cGAS signaling pathway. In summary, this study preliminarily clarified the molecular mechanism by which the ASFV MGF360-11L protein antagonizes IFN-I-mediated antiviral activity, which will help to provide new strategies for the treatment and prevention of ASF.
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http://dx.doi.org/10.1186/s13567-022-01025-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8785597PMC
January 2022

Photocatalysis of aqueous PFOA by common catalysts of InO, GaO, TiO, CeO and CdS: influence factors and mechanistic insights.

Environ Geochem Health 2022 Jan 22. Epub 2022 Jan 22.

School of Public Health and Emergency Management, Southern University of Science and Technology, Shenzhen, 518055, China.

Gallium oxide (GaO), titanium dioxide (TiO), cerium dioxide (CeO), indium oxide (InO) and cadmium sulfide (CdS) were commonly used under UV light as photocatalysis system for the pollutants' degradation. In this study, these five catalysts were applied for the photodegradation of perfluorooctanoic acid (PFOA), a well-known perfluoroalkyl substance (PFAS). As a result, the PFOA photodegradation performance was sequenced as: GaO > TiO > CeO > InO > CdS. To further explain the photocatalysis mechanism, the effects of initial pH, photon energy and band gap were evaluated. The initial pH of 3 ± 0.2 hinders the catalytic reaction of CdS, resulting in low degradation of PFOA, while it has no significant effect on GaO, TiO, CeO and InO. In addition, quantum yield was sequenced as TiO > CeO > GaO > InO, which may not be the main factor determining the degradation effect. Notably, the band gap energy from large to narrow was as: GaO > TiO > CeO > InO > CdS, which exactly matched their degradation performance.
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http://dx.doi.org/10.1007/s10653-021-01127-2DOI Listing
January 2022

Retraction of "Long non-coding RNA TUG1 knockdown hinders the tumorigenesis of multiple myeloma by regulating microRNA-34a-5p/NOTCH1 signaling pathway".

Open Life Sci 2021 21;16(1):1321. Epub 2021 Dec 21.

Department of Hematology, Ji'ning No. 1 People's Hospital, Ji'ning, Shandong, China.

[This retracts the article DOI: 10.1515/biol-2020-0025.].
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http://dx.doi.org/10.1515/biol-2021-0132DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8692659PMC
December 2021

Livelihoods, Technological Constraints, and Low-Carbon Agricultural Technology Preferences of Farmers: Analytical Frameworks of Technology Adoption and Farmer Livelihoods.

Int J Environ Res Public Health 2021 12 18;18(24). Epub 2021 Dec 18.

Grain Research Center, Nanjing Agricultural University, Nanjing 210005, China.

In the context of achieving carbon neutrality, it is scientifically important to quantitatively explore the relationships among livelihoods, technological property constraints, and the selection of low-carbon technologies by farmers to promote agricultural modernization and carbon neutrality in the agricultural sector of China. Based on the scientific classifications of farmer capital and low-carbon agricultural technologies, a farmer technology selection theory model considering capital constraints was developed in this study. Microcosmic survey data were collected from farmers in the Jiangsu province for empirical testing and analyses. A total of four low-carbon technologies related to fertilizer usage and three types of farmers' livelihoods and their relationships were examined by using a logistic model. The results showed the existence of a significant coupling relationship between the intrinsic decision mechanism involved in selecting low-carbon agricultural technology and the properties of low-carbon agricultural technology for different types of farmers. Significant differences exist in the selection of different low-carbon technologies among large-scale farmers, mid-level part-time farmers, and low-level (generally small) part-time farmers. (1) When selecting technology, large-scale farmers are more inclined to accept capital-intensive, low-carbon technologies, such as new varieties, straw recycling, soil testing, and formulated fertilization. Mid-level part-time farmers are more inclined to accept capital intensive, labor saving, or low risk low-carbon agricultural technologies. In contrast, low-level part-time farmers are inclined to accept labor intensive technologies to reduce capital constraints and agricultural risks. (2) Large-scale farmers and low-level part-time farmers are influenced by household and plot characteristics, while mid-level part-time farmers are more influenced by plot characteristics. (3) Households with capital constraints created by differentiated livelihoods face challenges adopting capital-intensive low-carbon agricultural technologies, such as straw recycling, new varieties, soil testing, and formulated fertilization. However, farmers with stronger constraints in the areas of land and labor are more inclined to accept labor-saving technologies, such as soil testing and formulated fertilization technology. Moreover, farmers with stronger risk preferences tend to accept high-risk technologies, such as new technologies like straw recycling. The results of this study can provide a scientific basis for formulating carbon emission reduction policies and low-carbon technology policies for the agricultural sector.
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http://dx.doi.org/10.3390/ijerph182413364DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8703629PMC
December 2021

Proteomic Changes in Sarcoplasmic and Myofibrillar Proteins Associated with Color Stability of Ovine Muscle during Post-Mortem Storage.

Foods 2021 Dec 3;10(12). Epub 2021 Dec 3.

College of Food Science and Nutritional Engineering, China Agricultural University, No. 17 Qinghua East Road, Haidian District, Beijing 100083, China.

The objective of this study was to investigate the proteomic characteristics for the sarcoplasmic and myofibrillar proteomes of (LL) and (PM) from Sheep. During post-mortem storage periods (1, 3, and 5 days), proteome analysis was applied to elucidate sarcoplasmic and myofibrillar protein changes in skeletal muscles with different color stability. Proteomic results revealed that the identified differentially abundant proteins were glycolytic enzymes, energy metabolism enzymes, chaperone proteins, and structural proteins. Through Pearson's correlation analysis, a few of those identified proteins (Pyruvate kinase, Adenylate kinase isoenzyme 1, Creatine kinase M-type, and Carbonic anhydrase 3) were closely correlated to representative meat color parameters. Besides, bioinformatics analysis of differentially abundant proteins revealed that the proteins mainly participated in glycolysis and energy metabolism pathways. Some of these proteins may have the potential probability to be predictors of meat discoloration during post-mortem storage. Within the insight of proteomics, these results accumulated some basic theoretical understanding of the molecular mechanisms of meat discoloration.
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http://dx.doi.org/10.3390/foods10122989DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8700786PMC
December 2021
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