Publications by authors named "Dandan Wang"

728 Publications

Efficacy and tolerability of pharmacological interventions on metabolic disturbance induced by atypical antipsychotics in adults: A systematic review and network meta-analysis.

J Psychopharmacol 2021 Jul 27:2698811211035391. Epub 2021 Jul 27.

Schizophrenia Program, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Background: There have been a few systematic reviews and conventional meta-analyses reporting effect of drugs on metabolic disturbance induced by atypical antipsychotics (AAPs). However, few of them provided sufficient and comprehensive comparisons between pharmacological interventions.

Aims: We aimed to qualitatively compare drugs' effect on AAPs-induced metabolic abnormalities by using network meta-analysis (NMA).

Methods: We searched PubMed, EMBASE, Web of Science, Cochrane Controlled Register of Trials (CENTRAL), and PsycINFO on March 26, 2019. Of 5889 records identified, 61 randomized clinical trials including 3467 participants were included. We estimated weighted mean difference (WMD) and odds ratio (OR) using NMA. We assessed the risk of bias of individual studies with the Review Manager 5.3. Primary outcomes included change of body weight and body mass index (BMI). Secondary outcomes included change of other cardiometabolic risk factors, acceptability, and tolerability.

Results: For body weight, topiramate (WMD -5.4, 95% CI -7.12 to -3.68), zonisamide (-3.44, 95% CI -6.57 to -0.36), metformin (-3.01, 95% CI -4.22 to -1.83), glucagon-like peptide-1 receptor agonists (GLP-1RAs) (-3.23, 95% CI -5.47 to -0.96), and nizatidine (-2.14, 95% CI -4.01 to -0.27) were significantly superior to placebo. Results regarding to BMI were similar to that of body weight. With respect to tolerability, only topiramate (OR 24, 95% CI 3.15 to 648) was inferior to placebo.

Conclusions: Considering both efficacy and tolerability, evidence from this NMA indicates zonisamide, metformin, GLP-1RAs, and nizatidine in adults should be the first-line treatment for alleviating AAPs-induced weight gain or elevated BMI.
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http://dx.doi.org/10.1177/02698811211035391DOI Listing
July 2021

Maternal Ethanol Exposure Acutely Elevates Src Family Kinase Activity in the Fetal Cortex.

Mol Neurobiol 2021 Jul 16. Epub 2021 Jul 16.

Department of Neuroscience and Physiology, SUNY Upstate Medical University, 505 Irving Ave, Syracuse, NY, 13210, USA.

Fetal alcohol syndrome (FAS) is characterized by disrupted fetal brain development and postnatal cognitive impairment. The targets of alcohol are diverse, and it is not clear whether there are common underlying molecular mechanisms producing these disruptions. Prior work established that acute ethanol exposure causes a transient increase in tyrosine phosphorylation of multiple proteins in cultured embryonic cortical cells. In this study, we show that a similar tyrosine phosphorylation transient occurs in the fetal brain after maternal dosing with ethanol. Using phospho-specific antibodies and immunohistochemistry, we mapped regions of highest tyrosine phosphorylation in the fetal cerebral cortex and found that areas of dendritic and axonal growth showed elevated tyrosine phosphorylation 10 min after maternal ethanol exposure. These were also areas of Src expression and Src family kinase (SFK) activation loop phosphorylation (pY416) expression. Importantly, maternal pretreatment with the SFK inhibitor dasatinib completely prevents both the pY416 increase and the tyrosine phosphorylation response. The phosphorylation response was observed in the perisomatic region and neurites of immature migrating and differentiating primary neurons. Importantly, the initial phosphotyrosine transient (~ 30 min) targets both Src and Dab1, two critical elements in Reelin signaling, a pathway required for normal cortical development. This initial phosphorylation response is followed by sustained reduction in Ser3 phosphorylation of n-cofilin, a critical actin severing protein and an identified downstream effector of Reelin signaling. This biochemical disruption is associated with sustained reduction of F-actin content and disrupted Golgi apparatus morphology in developing cortical neurons. The finding outlines a model in which the initial activation of SFKs by ethanol has the potential to disrupt multiple developmentally important signaling systems for several hours after maternal exposure.
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http://dx.doi.org/10.1007/s12035-021-02467-xDOI Listing
July 2021

Construction and Validation of a Lung Cancer Diagnostic Model Based on 6-Gene Methylation Frequency in Blood, Clinical Features, and Serum Tumor Markers.

Comput Math Methods Med 2021 26;2021:9987067. Epub 2021 Jun 26.

Department of Pathology, Henan Medical College, Zhengzhou, Henan Province, China 451191.

Lung cancer has a high mortality rate. Promoting early diagnosis and screening of lung cancer is the most effective way to enhance the survival rate of lung cancer patients. Through computer technology, a comprehensive evaluation of genetic testing results and basic clinical information of lung cancer patients could effectively diagnose early lung cancer and indicate cancer risks. This study retrospectively collected 70 pairs of lung cancer tissue samples and normal human tissue samples. The methylation frequencies of 6 genes (FHIT, p16, MGMT, RASSF1A, APC, DAPK) in lung cancer patients, the basic clinical information, and tumor marker levels of these patients were analyzed. Then, the python package "sklearn" was employed to build a support vector machine (SVM) classifier which performed 10-fold cross-validation to construct diagnostic models that could identify lung cancer risk of suspected cases. Receiver operation characteristic (ROC) curves were drawn, and the performance of the combined diagnostic model based on several factors (clinical information, tumor marker level, and methylation frequency of 6 genes in blood) was shown to be better than that of models with only one pathological feature. The AUC value of the combined model was 0.963, and the sensitivity, specificity, and accuracy were 0.900, 0.971, and 0.936, respectively. The above results revealed that the diagnostic model based on these features was highly reliable, which could screen and diagnose suspected early lung cancer patients, contributing to increasing diagnosis rate and survival rate of lung cancer patients.
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http://dx.doi.org/10.1155/2021/9987067DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8257360PMC
June 2021

Emerging Roles of Ubiquitin-Specific Protease 25 in Diseases.

Front Cell Dev Biol 2021 23;9:698751. Epub 2021 Jun 23.

Division of Pulmonary Medicine, The First Affiliated Hospital, Wenzhou Medical University, Wenzhou, China.

The balance of ubiquitination and deubiquitination plays diverse roles in regulating protein stability and cellular homeostasis. Deubiquitinating enzymes catalyze the hydrolysis and removal of ubiquitin chains from target proteins and play critical roles in various disease processes, including cancer, immune responses to viral infections and neurodegeneration. This article aims to summarize roles of the deubiquitinating enzyme ubiquitin-specific protease 25 (USP25) in disease onset and progression. Previous studies have focused on the role of USP25 in antiviral immunity and neurodegenerative diseases. Recently, however, as the structural similarities and differences between USP25 and its homolog USP28 have become clear, mechanisms of action of USP25 in cancer and other diseases have been gradually revealed.
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http://dx.doi.org/10.3389/fcell.2021.698751DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8262611PMC
June 2021

Comparative Microbial Profiles of Caries and Black Extrinsic Tooth Stain in Primary Dentition.

Caries Res 2021 Jul 9:1-12. Epub 2021 Jul 9.

Department of Paediatric Dentistry, Peking University School and Hospital of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology, Beijing, China.

Extrinsic black tooth stain (BS) is a common oral disease associated with lower caries experience in preschool children, although the microbiotic features contributing to the low risk of caries in this group remain elusive. In this study, we aimed at identifying the dominant bacteria in dental plaque to indicate the incidence of caries in the primary dentition. Subjects were divided into 3 groups based on the clinical examination: group CF, children without pigment who had no caries lesions or restorations (n = 18); group CS, children who were diagnosed with severe early childhood caries (n = 17); and group BS, children with pigment (black extrinsic stain) without caries or restorations (n = 15). The total microbial genomic DNA was extracted and subjected to bacterial 16S ribosomal RNA gene sequencing using an Illumina HiSeq platform. The differential dominant bacteria were determined using Wilcoxon rank-sum testing and linear discriminant analysis effect size (LEfSe). Co-occurrence network analysis was performed using sparse correlations for compositional data, calculation and functional features were predicted using PICRUSt. Interestingly, our results showed that the relative abundance of Pseudopropionibacterium, Actinomyces, Rothia, and Cardiobacterium was from high to low and that of Porphyromonas was low to high in the BS, CF, and CS groups, consistent with the clinical incidence of caries in the 3 groups. Moreover, an increased level of Selenomonas_3, Fusobacterium, and Leptotrichia was associated with high caries prevalence. We found that the interactions among genera in the BS and CS plaque communities are less complex than those in the CF communities at the taxon level. Functional features, including cofactor and vitamin metabolism, glycan biosynthesis and metabolism, and translation, significantly increased in caries plaque samples. These bacterial competition- and commensalism-induced changes in microbiota would result in a change of their symbiotic function, finally affecting the balance of oral microflora.
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http://dx.doi.org/10.1159/000517006DOI Listing
July 2021

MyD88 is an essential regulator of NK cell-mediated clearance of MCMV infection.

Mol Immunol 2021 Jul 6;137:94-104. Epub 2021 Jul 6.

Laboratory of Molecular Immunology and Immunotherapy, Blood Research Institute, Versiti, Milwaukee, WI, 53226, USA; Department of Microbiology and Immunology, Medical College of Wisconsin, Milwaukee, WI, 53226, USA; Department of Pediatrics, Medical College of Wisconsin, Milwaukee, WI, 53226, USA; Department of Medicine, Medical College of Wisconsin, Milwaukee, WI, 53226, USA. Electronic address:

The signaling adapter MyD88 is critical for immune cell activation in response to viral or bacterial pathogens via several TLRs, IL-1βR and IL-18R. However, the essential role of MyD88 during activations mediated by germline-encoded NK cell receptors (NKRs), such as Ly49H or NKG2D, has yet to be investigated. To define the NK cell-intrinsic function of MyD88, we generated a novel NK cell conditional knockout mouse for MyD88 (Myd88Ncr1). Phenotypic characterization of these mice demonstrated that MyD88 is dispensable for NK cell development and maturation. However, the MyD88-deficient NK cells exhibited significantly reduced cytotoxic potentials in vivo. In addition, the lack of MyD88 significantly reduced the NKG2D-mediated inflammatory cytokine production in vitro. Consistent with this, mice lacking MyD88 were unable to respond and clear MCMV infection. Transcriptomic analyses of splenic NK cells following MCMV infection revealed that inflammatory gene signatures were upregulated in Ly49H. In contrast, Ly49H NK cells have significant enrichment in G2M checkpoint genes, revealing distinct transcriptomic profiles of these subsets. Our results identify a central role for MyD88 in Ly49H-dependent gene signatures, including alterations in genes regulating proliferation in Ly49H NK cells. In summary, our study reveals a previously unknown function of MyD88 in Ly49H-dependent signaling and in vivo functions of NK cells.
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http://dx.doi.org/10.1016/j.molimm.2021.07.001DOI Listing
July 2021

Malignant transformation of hepatic endometriosis: a case report and literature review.

BMC Womens Health 2021 06 21;21(1):249. Epub 2021 Jun 21.

Department of Pathology, Shengjing Hospital of China Medical University, No. 36 Sanhao Street, Heping District, Shenyang, 110004, China.

Background: Extrapelvic endometriosis is defined as the presence of ectopic endometrial tissue in structures outside the pelvis. Although extra-pelvic endometriosis is generally considered benign conditions, malignant potential within endometriotic foci occurs even after definitive surgery. Malignant transformation of hepatic endometriosis is extremely rare. Preoperative diagnosis of this cancer is difficult, and no guidelines on the optimal management currently exist. Here, we present a case report of malignant transformation of hepatic endometriosis and a brief literature review to highlight the current knowledge of the prevalence, clinical features, diagnosis, and management of this condition.

Case Presentation: A 50-year-old woman with a 2-year duration of progressive right upper quadrant abdominal pain was admitted to the hospital. She underwent hysterectomy and bilateral salpingo-oophorectomy for benign conditions 4 years prior. Tumor markers demonstrated elevated carbohydrate antigen (CA)-199 112U/mL (normal range: 0-35U/mL) only. Radiological imaging suggested the presence of a 10.7 × 7.7-cm mass in the right lobe of the liver extending to the diaphragm. The intraoperative frozen sections suggested malignant tumor. Right hepatectomy with infiltrating diaphragm resection was performed. The final pathology with immunohistochemistry staining confirmed endometrioid adenocarcinoma in the liver originating from preexisting hepatic endometriosis. After the multidisciplinary team meeting, the consensus was surgery followed by adjuvant chemotherapy. To our knowledge, this is the first case of Chinese woman of a malignant liver tumor originating from endometriosis ever reported by reviewing the current English medical literature.

Conclusion: Though rare, extrapelvic endometriosis-associated cancers should be considered as differentiated diagnosis even after hysterectomy and bilateral salpingo-oophorectomy. This case highlights the importance of collaborative efforts across multiple disciplines for accurate diagnosis and appropriate treatment of malignant transformation of hepatic endometriosis.
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http://dx.doi.org/10.1186/s12905-021-01366-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8218461PMC
June 2021

Serum galectin-3 level as a marker for diagnosis and prognosis of neonatal necrotising enterocolitis: a cohort study.

Am J Transl Res 2021 15;13(5):5731-5737. Epub 2021 May 15.

Department of Gastroenterology, The Second Affiliated Hospital of Zhengzhou University Zhengzhou 450000, Henan, China.

Objective: Neonatal necrotising enterocolitis (NEC) is a leading factor in neonatal mortality. Diagnosing NEC is difficult since it presents with various clinical appearances with divergent symptoms. This study determines the value of galectin-3 (GAL-3) for diagnosing NEC.

Methods: Seventy-two newborn patients with NEC and 64 preterm infants with jaundice (control group) were prospectively enrolled. The levels of white blood cells (WBC), C-reactive protein (CRP), procalcitonin (PCT), intestinal fatty acid binding protein (I-FABP), serum cytosolic β-glycosidase (CBG), and GAL-3 in the serum were measured. In addition, the diagnostic values of GAL-3 for diagnosing early and severe NEC were analysed by a receiver operating characteristic curve.

Results: WBC, CRP, PCT, I-FABP, CBG, and GAL-3 showed an increasing trend in the control, NEC I, and NEC II+III groups. Moreover, in the diagnosis of early and severe NEC, GAL-3 had a higher sensitivity and specificity than WBC, CRP, PCT, I-FABP, and CBG. The results also suggest that the GAL-3 level is an independent prognostic measure to indicate poor prognosis in NEC.

Conclusion: GAL-3 is a useful marker for diagnosing and prognosis of neonatal necrotising enterocolitis.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8205674PMC
May 2021

COVID-19 Information Dissemination Using the WeChat Communication Index: Retrospective Analysis Study.

J Med Internet Res 2021 07 16;23(7):e28563. Epub 2021 Jul 16.

School of Management, Weifang Medical University, Weifang, Shandong, China.

Background: The COVID-19 outbreak has tremendously impacted the world. The number of confirmed cases has continued to increase, causing damage to society and the economy worldwide. The public pays close attention to information on the pandemic and learns about the disease through various media outlets. The dissemination of comprehensive and accurate COVID-19 information that the public needs helps to educate people so they can take preventive measures.

Objective: This study aimed to examine the dissemination of COVID-19 information by analyzing the information released by the official WeChat account of the People's Daily during the pandemic. The most-read COVID-19 information in China was summarized, and the factors that influence information dissemination were studied to understand the characteristics that affect its dissemination. Moreover, this was conducted in order to identify how to effectively disseminate COVID-19 information and to provide suggestions on how to manage public opinion and information governance during a pandemic.

Methods: This was a retrospective study based on a WeChat official account. We collected all COVID-19-related information, starting with the first report about COVID-19 from the People's Daily and ending with the last piece of information about lifting the first-level emergency response in 34 Chinese provinces. A descriptive analysis was then conducted on this information, as well as on Qingbo Big Data's dissemination index. Multiple linear regression was utilized to study the factors that affected information dissemination based on various characteristics and the dissemination index.

Results: From January 19 to May 2, 2020, the People's Daily released 1984 pieces of information; 1621 were related to COVID-19, which mainly included headline news items, items with emotional content, and issues related to the pandemic's development. By analyzing the dissemination index, seven information dissemination peaks were discerned. Among the three dimensions of COVID-19 information-media salience, content, and format-eight factors affected the spread of COVID-19 information.

Conclusions: Different types of pandemic-related information have varying dissemination power. To effectively disseminate information and prevent the spread of COVID-19, we should identify the factors that affect this dissemination. We should then disseminate the types of information the public is most concerned about, use information to educate people to improve their health literacy, and improve public opinion and information governance.
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http://dx.doi.org/10.2196/28563DOI Listing
July 2021

MPDZ as a novel epigenetic silenced tumor suppressor inhibits growth and progression of lung cancer through the Hippo-YAP pathway.

Oncogene 2021 Jul 9;40(26):4468-4485. Epub 2021 Jun 9.

Institute of Toxicology, College of Preventive Medicine, Third Military Medical University (Army Medical University), Chongqing, PR China.

MPDZ also named MUPP1 is involved in signal transduction mediated by the formation of protein complexes. However, the expression regulation, clinical significance, potential function, and mechanism of this gene in lung cancer remain unclear. Methylation status of MPDZ was measured by methylation-specific PCR and bisulfite genomic sequencing. Kaplan-Meier and Cox regression analyses were performed to identify the prognostic value of MPDZ. The tumor suppressing effects of MPDZ were determined in vitro and in vivo. The target molecules and signaling pathway that mediated the function of MPDZ were also identified. MPDZ methylation was identified in 61.2% of primary lung cancer tissues and most lung cancer cell lines but not in normal lung tissues. MPDZ expression was significantly downregulated in lung cancer tissues and negatively associated with DNA hypermethylation, and attenuated MPDZ expression predicted a poor outcome. Furthermore, MPDZ overexpression prominently dampened cell growth, migration, and invasion of tumor cells. Conversely, MPDZ knockdown promoted cell proliferation, migration, and invasion in vitro and in vivo. Moreover, MPDZ deficiency promotes tumor metastasis and reduces the survival of MPDZ knockout mice. Importantly, MPDZ promotes tumor suppressor ability that depends on the Hippo pathway-mediated repression of YAP. MPDZ activates the phosphorylation of YAP (Ser127) and inhibits YAP expression through stabilizing MST1 and interaction with LATS1. We first identified and validated that MPDZ methylation and expression could be a good diagnostic marker and independent prognostic factor for lung cancer. MPDZ functions as a tumor suppressor by inhibiting cell proliferation, migration, and invasion through regulating the Hippo-YAP signaling pathway.
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http://dx.doi.org/10.1038/s41388-021-01857-8DOI Listing
July 2021

Global investigation of estrogen-responsive genes regulating lipid metabolism in the liver of laying hens.

BMC Genomics 2021 Jun 9;22(1):428. Epub 2021 Jun 9.

College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450002, China.

Background: Estrogen plays an essential role in female development and reproductive function. In chickens, estrogen is critical for lipid metabolism in the liver. The regulatory molecular network of estrogen in chicken liver is poorly understood. To identify estrogen-responsive genes and estrogen functional sites on a genome-wide scale, we determined expression profiles of mRNAs, lncRNAs, and miRNAs in estrogen-treated ((17β-estradiol)) and control chicken livers using RNA-Sequencing (RNA-Seq) and studied the estrogen receptor α binding sites by ChIP-Sequencing (ChIP-Seq).

Results: We identified a total of 990 estrogen-responsive genes, including 962 protein-coding genes, 11 miRNAs, and 17 lncRNAs. Functional enrichment analyses showed that the estrogen-responsive genes were highly enriched in lipid metabolism and biological processes. Integrated analysis of the data of RNA-Seq and ChIP-Seq, identified 191 genes directly targeted by estrogen, including 185 protein-coding genes, 4 miRNAs, and 2 lncRNAs. In vivo and in vitro experiments showed that estrogen decreased the mRNA expression of PPARGC1B, which had been reported to be linked with lipid metabolism, by directly increasing the expression of miR-144-3p.

Conclusions: These results increase our understanding of the functional network of estrogen in chicken liver and also reveal aspects of the molecular mechanism of estrogen-related lipid metabolism.
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http://dx.doi.org/10.1186/s12864-021-07679-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8190866PMC
June 2021

A catalytic triplex DNAzyme for porphyrin metalation.

Chem Commun (Camb) 2021 Jul;57(53):6499-6502

Key Laboratory of the Ministry of Education for Advanced Catalysis Materials, Institute of Physical Chemistry, College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, Zhejiang, China.

Trihydroxyphenyl porphyrin (POH3) was designed to specifically bind with a triplex DNA resulting in a turn-on fluorescence response. This ensemble can be developed into a catalytic triplex DNAzyme towards porphyrin metalation. The catalytic activity is initiated by the enhanced basicity of POH3 upon binding with the triplex DNA.
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http://dx.doi.org/10.1039/d1cc01955dDOI Listing
July 2021

Comparative analysis of hypothalamus transcriptome between laying hens with different egg-laying rates.

Poult Sci 2021 Jul 11;100(7):101110. Epub 2021 Mar 11.

College of Animal Science, Henan Agricultural University, Zhengzhou 450046, China; Henan Innovative Engineering Research Center of Poultry Germplasm Resource, Henan Agricultural University, Zhengzhou 450046, China; International Joint Research Laboratory for Poultry Breeding of Henan, Henan Agricultural University, Zhengzhou 450046, China. Electronic address:

Egg-laying performance is one of the most important economic traits in the poultry industry. Commercial layers can lay one egg almost every day during their peak-laying period. However, many Chinese indigenous chicken breeds show a relatively low egg-laying rate, even during their peak-laying period. To understand what makes the difference in egg production, we compared the hypothalamus transcriptome profiles of Lushi blue-shelled-egg chickens (LBS), a Chinese indigenous breed with low egg-laying rate and Rhode Island Red chickens (RIR), a commercial layer with relatively high egg-laying rate using RNA-seq. A total of 753 differentially expressed genes (DEGs) were obtained. Of these DEGs, 38 genes were enriched in 2 Gene Ontology (GO) terms, namely reproduction term and the reproductive process term, and 6 KEGG pathways, namely Wnt signaling pathway, Oocyte meiosis, GnRH signaling pathway, Thyroid hormone signaling pathway, Thyroid hormone synthesis and MAPK signaling pathway, which have been long known to be involved in egg production regulation. To further determine the core genes from the 38 DEGs, protein-protein interaction (PPI) network, co-expression network and transcriptional regulatory network analyses were carried out. After integrated analysis and experimental validation, 4 core genes including RAC1, MRE11A, MAP7 and SOX5 were identified as the potential core genes that are responsible for the laying-rate difference between the 2 breeds. These findings paved the way for future investigating the mechanism of egg-laying regulation and enriched the chicken reproductive regulation theory.
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http://dx.doi.org/10.1016/j.psj.2021.101110DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8187251PMC
July 2021

Study on the prognosis predictive model of COVID-19 patients based on CT radiomics.

Sci Rep 2021 06 2;11(1):11591. Epub 2021 Jun 2.

Department of Radiology, Longhua Hospital,, Shanghai University of Traditional Chinese Medicine, No.725, South Wanping Road, Shanghai, 200032, China.

Making timely assessments of disease progression in patients with COVID-19 could help offer the best personalized treatment. The purpose of this study was to explore an effective model to predict the outcome of patients with COVID-19. We retrospectively included 188 patients (124 in the training set and 64 in the test set) diagnosed with COVID-19. Patients were divided into aggravation and improvement groups according to the disease progression. Three kinds of models were established, including the radiomics, clinical, and combined model. Receiver operating characteristic curves, decision curves, and Delong's test were used to evaluate and compare the models. Our analysis showed that all the established prediction models had good predictive performance in predicting the progress and outcome of COVID-19.
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http://dx.doi.org/10.1038/s41598-021-90991-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8172890PMC
June 2021

Analysis of the Storage Stability Property of Carbon Nanotube/Recycled Polyethylene-Modified Asphalt Using Molecular Dynamics Simulations.

Polymers (Basel) 2021 May 20;13(10). Epub 2021 May 20.

College of Civil Engineering, Henan University of Technology, Zhengzhou 450001, China.

Carbon nanotubes (CNTs) can improve the storage properties of modified asphalt by enhancing the interfacial adhesion of recycled polyethylene (RPE) and base asphalt. In this study, the interaction of CNT/RPE asphalt was investigated using molecular dynamics simulation. The base asphalt was examined using a 12-component molecular model and verified by assessing the following properties: its four-component content, elemental contents, radial distribution function (RDF) and glass transition temperature. Then, the adhesion properties at the interface of the CNT/RPE-modified asphalt molecules were studied by measuring binding energy. The molecular structural stability of CNTs at the interface between RPE and asphalt molecules was analyzed through the relative concentration distribution. The motion of molecules in the modified asphalt was studied in terms of the mean square displacement (MSD) and diffusion coefficient. The results showed that CNTs improved the binding energy between RPE and base asphalt. CNTs not only weakened the repulsion of RPE with asphaltenes and resins, but also promoted the interaction of RPE with light components, which facilitated the compatibility of RPE with the base asphalt. The change in the interaction affected the molecular motion, and the molecular diffusion coefficient in the CNT/RPE-modified asphalt system was significantly smaller than that of RPE-modified asphalt. Moreover, the distribution of the asphaltene component was promoted by CNTs, resulting in the enhancement of the storage stability of RPE-modified asphalt. The property indexes indicated that the storage stability was significantly improved by CNTs, and better viscoelastic properties were also observed. Our research provides a foundation for the application of RPE in pavement engineering.
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http://dx.doi.org/10.3390/polym13101658DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8160613PMC
May 2021

The integration of transcriptomic and transgenic analyses reveals the involvement of the SA response pathway in the defense of chrysanthemum against the necrotrophic fungus Alternaria sp.

Hortic Res 2020 Jun 1;7(1):80. Epub 2020 Jun 1.

College of Life Sciences, Henan Normal University, Xinxiang, 453007, China.

Chrysanthemum morifolium cv. 'Huaihuang' has ornamental, edible, medicinal, and tea product uses. However, its field growth, yield, and quality are negatively affected by black spot disease caused by Alternaria sp. (Strain: HQJH10092301; GenBank accession number: KF688111). In this study, we transcriptionally and transgenically characterized a new cultivar, 'Huaiju 2' (Henan Traditional Chinese Medicine Plant Cultivar identification number: 2016002), which was bred from 'Huaihuang' and shows resistance to Alternaria sp. Numerous 'Huaiju 2' plants were inoculated with Alternaria sp. for three or five days. Metabolic analysis showed increases in both salicylic acid (SA) and jasmonic acid (JA) in infected plants compared to the control. Protein activity analysis also revealed a significant increase in defense enzyme activities in infected plants. RNA-Seq of plants infected for 3 or 5 days produced a total of 58.6 GB of clean reads. Among these reads, 16,550 and 13,559 differentially expressed genes (DEGs) were identified in Cm_3 dpi (sample from 3 days post-inoculation labeled as Cm_3 dpi) and Cm_5 dpi (sample from 5 days post-inoculation labeled as Cm_5 dpi), respectively, compared with their controls (Cm_0 d: a mixture samples from 0 d (before inoculation) and those treated with sterile distilled water at 3 dpi and 5 dpi). Gene annotation and cluster analysis of the DEGs revealed a variety of defense responses to Alternaria sp. infection, which were characterized by increases in resistance (R) proteins and the reactive oxygen species (ROS), Ca, mitogen-activated protein kinase (MAPK), and JA signaling pathways. In particular, SA signaling was highly responsive to Alternaria sp. infection. The qPCR analysis of 12 DEG candidates supported their differential expression characterized by using the RNA-Seq data. One candidate was CmNPR1 (nonexpressor of pathogenesis-related gene 1), an important positive regulator of SA in systemic acquired resistance (SAR). Overexpression of CmNPR1 in 'Huaiju 2' increased the resistance of transgenic plants to black spot. These findings indicate that the SA response pathway is likely involved in the defense of 'Huaiju 2' against Alternaria sp. pathogens.
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http://dx.doi.org/10.1038/s41438-020-0297-1DOI Listing
June 2020

Relationship between individual alpha peak frequency and attentional performance in a multiple object tracking task among ice-hockey players.

PLoS One 2021 27;16(5):e0251443. Epub 2021 May 27.

School of Psychology, Shanghai University of Sport, Shanghai, China.

Individual alpha peak frequency (IAPF), the discrete frequency with the highest power value in the alpha oscillation range of the electroencephalogram, is a stable neurophysiological marker and is closely associated with various cognitive functions, including aspects of attention and working memory. However, the relationship between IAPF and attentional performance as well as the effects of engaging attention on IAPF are unknow. Here, we examined whether IAPF values were associated with attentional performance by evaluating accuracy during the performance of a multiple object tracking (MOT) task, a well-established paradigm for investigating goal-driven attention in dynamic environments, and whether engagement in the task affected IAPF values. In total, 18 elite players and 20 intermediate players completed the study. Resting electroencephalogram recordings were obtained for 120 s while players kept their eyes open and an additional 120 s while players' eyes were closed, before and again after performing the MOT task. Tracking accuracy in the MOT task and IAPF values before and after the MOT task were analyzed. As expected, tracking accuracies were higher in elite players than in intermediate-level players. Baseline IAPF values were significantly and positively correlated with the accuracy of target tracking in the MOT task. IAPF values were higher in elite than intermediate players in both the eyes open and closed conditions and both before and after MOT task performance. Interindividual IAPF values did not differ before and after the MOT task. These findings indicate that IAPF is a stable marker, without intraindividual changes associated with engagement in the MOT task. Elite players had higher IAPF values and exhibited more accurate MOT performance than intermediate-level players; thus, baseline IAPF values may be useful to predict attentional performance in the MOT task among athletes.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0251443PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8158945PMC
May 2021

cRGD peptide-conjugated polyethylenimine-based lipid nanoparticle for intracellular delivery of siRNA in hepatocarcinoma therapy.

Drug Deliv 2021 Dec;28(1):995-1006

School of Pharmacy, Shanxi Medical University, Taiyuan, China.

The effective delivery system plays an important role in the application of siRNA in the antitumor study. However, until now, researches on the delivery systems targeting hepatocarcinoma cells are still being explored. Here we designed and prepared a novel siRNA delivery system, cRGD-PSH-NP, which was based on a modified polyethyleneimine (PSH) and DSPE-PEG-cRGD. cRGD-PSH-NP loaded with survivin siRNA (cRGD-PSH-NP/S) was composed of egg phosphatidylcholine, cationic PSH, PEGylated lipids, survivin siRNA, and cRGD peptide as a targeting ligand. The formulations of cRGD-PSH-NP/S were optimized and characterized. investigations showed excellent gene silencing and antitumor activity compared with the unmodified nanoparticles in HepG2 cells. antitumor efficacy of cRGD-PSH-NP/S exhibited potent tumor inhibition (74.71%) in HepG2-bearing nude mice without inducing toxicity. These data suggested further research of cRGD-PSH-NP/S in hepatocarcinoma therapy.
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http://dx.doi.org/10.1080/10717544.2021.1928794DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8168781PMC
December 2021

Reduction of N to NH by TiO-supported Ni cluster catalysts: a DFT study.

Phys Chem Chem Phys 2021 May 25. Epub 2021 May 25.

Department of Chemical Engineering, University of Waterloo, 200 University Avenue West, Waterloo, ON N2L3G1, Canada.

Electrochemical techniques for ammonia synthesis are considered as an encouraging energy conversion technology to efficiently meet the challenge of nitrogen cycle balance. Herein, we find that TiO2(101)-supported Ni4 and Ni13 clusters can serve as efficient catalysts for electrocatalytic N2 reduction based on theoretical calculations. Electronic property calculations reveal the formation of electron-deficient Ni clusters on the TiO2 surface, which provides multiple active sites for N2 adsorption and activation. Theoretical calculation identifies the strongest activated configuration of N2* on the catalysts and confirms the potential-limiting step in the nitrogen reduction reaction (NRR). On Ni4-TiO2(101), N2* → NNH* is the potential-limiting step with a very small free energy increase (ΔG) of 0.24 eV (the corresponding overpotential is 0.33 V), while on Ni13-TiO2(101) the potential-limiting step occurs at NH* → NH2* with ΔG of 0.49 eV (the corresponding overpotential is 0.58 V). Moreover, the Nin-TiO2(101) catalyst, especially Ni13-TiO2(101), involves in a highly selective NRR even at the corresponding NRR overpotential. This work will enlighten material design to construct metal oxide supported transition metal clusters for the highly efficient NRR and NH3 synthesis.
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http://dx.doi.org/10.1039/d1cp00859eDOI Listing
May 2021

Co-infection analysis of bacterial and viral respiratory pathogens from clinically healthy swine in Eastern China.

Vet Med Sci 2021 May 25. Epub 2021 May 25.

Institute of Veterinary Medicine, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing, China.

Porcine respiratory disease complex (PRDC) is one of the most challenging health concerns for pig production worldwide. The aim of the present study was to determine the prevalence of pathogens associated with PRDC, including porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2) and bacterial agents, such as Streptococcus suis, Haemophilus parasuis and Actinobacillus pleuropneumoniae, in clinically healthy pigs in Eastern China. Molecular detection revealed positive single-pathogen detection rates of 59.9%, 27.2%, 52.3%, 33.2% and 0.4% for PCV2, PRRSV, S. suis, H. parasuis and A. pleuropneumoniae, respectively. Co-infection with more than one pathogen was frequently detected in these samples, with PCV2/S. suis, H. parasuis and PCV2/H. parasuis mixed infection rates of 35.4%, 33.2% and 21.6%, respectively, and PCV2/S. suis/H. parasuis and PRRSV/PCV2/S. suis co-infection rates of 21.6% and 6.2%, respectively. These results suggest that mixed infections are prevalent among PRDC cases in swine, which may pose a greater threat to the health of herds compared with single-pathogen infections.
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http://dx.doi.org/10.1002/vms3.533DOI Listing
May 2021

Improving stability and oral bioavailability of hydroxycamptothecin via nanocrystals in microparticles (NCs/MPs) technology.

Int J Pharm 2021 Jul 23;604:120729. Epub 2021 May 23.

School of Pharmacy, Shanghai University of Medicine & Health Sciences, Shanghai 201318, China. Electronic address:

This study developed a nanocrystals-in-microparticles (NCs/MPs) technology for improving dissolution and oral absorption of poorly soluble drugs. Hydroxycamptothecin (HCPT) was selected as a model drug and prepared to be nanocrystals (HCPT-NCs) by acid-alkali based precipitation. The HCPT-NCs were rod like shape with the length of 250 nm and the width of 50 nm. Then, the chitosan and sodium alginate were selected as microparticles matrix to encapsulate the HCPT-NCs. The HCPT-NCs were entrapped in microparticles with a D value of 15 µm. The drug loading capacity of microparticles achieved more than 40% (w/w) by NCs/MPs technology. The powder X-ray diffraction showed the crystal structure of HCPT in microparticles was same as nanocrystals, indicating that the preparation of microparticles could not destroy the nanocrystals. The in vitro release demonstrated that microparticles could protect the NCs in gastric fluid and release NCs in intestinal fluid. Furthermore, the oral bioavailability of HCPT in NCs/MPs was improved by 18-fold compared to bulk HCPT and 2.1-fold compared to HCPT-NCs as tested by a rat model. Therefore, NCs/MPs technology is a promising and high effective approach to improve the oral bioavailability of poorly soluble drugs.
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http://dx.doi.org/10.1016/j.ijpharm.2021.120729DOI Listing
July 2021

Quantitative proteomic analysis reveals that serine/threonine kinase is involved in Streptococcus suis virulence and adaption to stress conditions.

Arch Microbiol 2021 May 24. Epub 2021 May 24.

Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, People's Republic of China.

The eukaryotic-type serine/threonine kinase of Streptococcus suis serotype 2 (SS2) performs critical roles in bacterial pathogenesis. In this study, isobaric tags for relative and absolute quantification (iTRAQ) MS/MS were used to analyze the protein profiles of wild type strain SS2-1 and its isogenic STK deletion mutant (Δstk). A total of 281 significant differential proteins, including 147 up-regulated and 134 down-regulated proteins, were found in Δstk. Moreover, 69 virulence factors (VFs) among these 281 proteins were predicted by the Virulence Factor Database (VFDB), including 38 downregulated and 31 up-regulated proteins in Δstk, among which 15 down regulated VFs were known VFs of SS2. Among the down-regulated proteins, high temperature requirement A (HtrA), glutamine synthase (GlnA), ferrichrome ABC transporter substrate-binding protein FepB, and Zinc-binding protein AdcA are known to be involved in bacterial survival and/or nutrient and energy acquisition under adverse host conditions. Overall, our results indicate that STK regulates the expression of proteins involved in virulence of SS2 and its adaption to stress environments.
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http://dx.doi.org/10.1007/s00203-021-02369-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8141825PMC
May 2021

Epidemiological Characteristics of Influenza A and B in Macau, 2010-2018.

Virol Sin 2021 May 20. Epub 2021 May 20.

Faculty of Health Sciences, University of Macau, Macau, 999078, Macau.

Influenza is one of the major respiratory diseases in humans. Macau is a tourist city with high density of population and special population mobility. The study on the epidemiological characteristics of influenza in Macau should bring great value for preventing influenza in tourist cities like Macau in the world. In this study, we collected a total of 104,874 samples with influenza-like illness (ILI) in Macau from 2010 to 2018. Chi-square test and binary multivariable logistic regression were used to investigate the epidemiological characteristics of influenza A and B in Macau. Among these ILI samples, the overall positive rate is 17.17% for influenza A and 6.97% for influenza B. The epidemics of influenza in three years (i.e., 2012, 2017 and 2018) differ from the remaining years (i.e., normal years). In a normal year, influenza A occurs year-round whereas influenza B is seasonal. Our research shows significant differences in influenza infections between different age groups in normal years. Interestingly, our analysis shows no significant difference between locals and tourists in influenza A and B infection in a normal year, whereas the odds of influenza A in tourists were significantly higher than those in locals in July 2017 and the odds of influenza B in tourists were significantly higher than those in locals in January-February 2012 and January-February 2018. This is possibly attributed by the policy of free vaccination to everyone in Macau. These findings should be valuable for preventing influenza in not only Macau but also the world.
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http://dx.doi.org/10.1007/s12250-021-00388-6DOI Listing
May 2021

The ROBINS-I and the NOS had similar reliability but differed in applicability: A random sampling observational studies of systematic reviews/meta-analysis.

J Evid Based Med 2021 May 18;14(2):112-122. Epub 2021 May 18.

Department of Evidence-based Medicine and Clinical Epidemiology, West China Hospital, Sichuan University, Chengdu, Sichuan, China.

Objective: There is a lack of evidence on the usage of the quality assessment tool-the Risk Of Bias In Nonrandomized Studies-of Interventions (ROBINS-I). This article aimed to measure the reliability, criterion validity, and feasibility of the ROBINS-I and the Newcastle-Ottawa Scale (NOS).

Methods: A sample of systematic reviews or meta-analyses of observational studies were selected from Medline (2013-2017) and assessed by two reviewers using ROBINS-I and the NOS. We reported on reliability in terms of the first-order agreement coefficient (AC1) statistic. Correlation coefficient statistic was used to explore the criterion validity of the ROBINS-I. We compared the feasibility of the ROBINS-I and NOS by recording the time to complete an assessment and the instances where assessing was difficult.

Results: Five systematic reviews containing 41 cohort studies were finally included. Interobserver agreement on the individual domain of the ROBINS-I as well as the NOS was substantial with a mean AC1 statistic of 0.67 (95% CI: 0.50-0.83) and 0.73 (95% CI: 0.65-0.81), respectively. The criterion validity of the ROBNS-I was moderate (K = 0.52) against NOS. The time in assessing a single study by ROBINS-I varied from 7 hours initially to 3 hours compared with 30 minutes for the NOS. Both reviewers rated "bias due to departure from the intended interventions" the most time-consuming domain in the ROBINS-I, items in the NOS were equal.

Conclusions: The ROBINS-I and the NOS seem to provide the same reliability but vary in applicability. The over-complicated feature of ROBINS-I may limit its usage and a simplified version is needed.
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http://dx.doi.org/10.1111/jebm.12427DOI Listing
May 2021

Multiscale mechanisms of asphalt performance enhancement by crumbed waste tire rubber: insight from molecular dynamics simulation.

J Mol Model 2021 May 17;27(6):170. Epub 2021 May 17.

School of Civil and Architectural Engineering, Shandong University of Technology, Zibo, 255049, China.

The recycling of waste tires is a major environmental problem facing mankind, and the addition of crumbed waste tire rubber (CWTB) to the base asphalt is an extremely promising recycling method. However, the modification mechanism of CWTB to asphalt is not well understood, which restricts the development of CWTB modified asphalt. In this study, the mechanism of CWTB modification of asphalt was explored by dynamic mechanical analysis (DMA), fluorescence microscopy, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and molecular dynamic (MD) simulations. After verifying the asphalt reasonableness using glass transition temperature, CWTB modified asphalt was simulated and experimented. The results showed that CWTB enhanced the high temperature performance of the base asphalt. The microscopic mechanism by which this phenomenon occurs is that CWTB has the largest binding energy with the aromatics (1150-1350 kcal/mol), followed by the saturates (740-830 kcal/mol), followed by the resins (90-330 kcal/mol), and the smallest binding energy with the asphaltenes (100-140 kcal/mol), which causes CWTB to absorb the light components of the asphalt (aromatics and saturates). In addition, the introduction of CWTB reduces the diffusion coefficient of asphalt. In this process, CWTB will gradually swell, which causes CWTB to bind more and more tightly with the base asphalt, and eventually the good high temperature performance of CWTB is transferred to the base asphalt. The macroscopic manifestation of this process is that the rutting factor of CWTB-modified asphalt is significantly higher than that of virgin asphalt. This study can provide basic theoretical support for the application of CWTB-modified asphalt.
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http://dx.doi.org/10.1007/s00894-021-04786-1DOI Listing
May 2021

Adipose-mesenchymal stromal cells suppress experimental Sjögren syndrome by IL-33-driven expansion of ST2 regulatory T cells.

iScience 2021 May 16;24(5):102446. Epub 2021 Apr 16.

Mucosal Immunology Section, NIDCR, NIH, Bethesda, MD 20892, USA.

Adipose-derived mesenchymal stromal cells (ADSCs) play important roles in the alleviation of inflammation and autoimmune diseases. Interleukin-33 (IL-33), a member of the IL-1 family, has been shown to regulate innate and adaptive immunity. However, it is still unknown whether ADSCs regulate immune responses via IL-33. We show here that ADSCs produced IL-33 in response to IL-1β stimulation, which depended on TAK1, ERK, and p38 pathways. ADSCs-derived IL-33 drove the proliferation of CD4Foxp3ST2 regulatory T cells (Tregs) and alleviated experimental autoimmune Sjögren syndrome in mice. Importantly, human ADSCs also produced IL-33 in response to IL-1β. Thus, we have revealed a previously unrecognized immunoregulatory function of ADSCs by IL-33 production in experimental autoimmunity, which may have clinical applications for human immunopathology.
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http://dx.doi.org/10.1016/j.isci.2021.102446DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8105666PMC
May 2021

The transcriptional regulator Zur regulates the expression of ZnuABC and T6SS4 in response to stresses in Yersinia pseudotuberculosis.

Microbiol Res 2021 Aug 10;249:126787. Epub 2021 May 10.

State Key Laboratory of Crop Stress Biology for Arid Areas, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University, Yangling, 712100, China. Electronic address:

Zinc homeostasis is crucial for the development and stress resistance of bacteria in the environment. Serial zinc sensing transcriptional regulators, zinc transporters and zinc binding proteins were found to maintain the zinc homeostasis in bacteria. Zur is a zinc uptake regulator that is widely distributed in species, and ZnuABC, as well as the Type VI Secretion System (T6SS4) function in zinc acquisition. Here, we report that the regulator Zur inhibits the expression of the ZnuABC which inhibition could be eliminated at low zinc level, and upregulates the T6SS4 operon in Yersinia pseudotuberculosis to facilitate Zn uptake and oxidative stress resistance. Zur regulates the expression of ZnuABC and T6SS4 by directly binding to their promoter regions. Zur senses the Zn concentration and represses ZnuABC in a Zn-containing environment. Zur works as an auxiliary regular activator of T6SS4, facilitating oxidative stress resistance. This study revealed the dual function of regulator Zur on ZnuABC and T6SS4, and enriched the knowledge of Zn homeostasis maintenance in Y. pseudotuberculosis.
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http://dx.doi.org/10.1016/j.micres.2021.126787DOI Listing
August 2021

Effects of a one-year physical activity intervention on fundamental movement skills of boys with severe intellectual disabilities.

Res Dev Disabil 2021 Jul 10;114:103980. Epub 2021 May 10.

School of Physical Education and Sport Training, Shanghai University of Sport, Shanghai 200438, China. Electronic address:

Background: Little is known about the effectiveness of using physical activity-based interventions to improve fundamental movement skills (FMS) of children with severe intellectual disabilities (ID).

Aims: The purpose of this study was to examine the effect of a one-year physical activity intervention on FMS among boys with severe ID.

Methods And Procedures: Forty-two boys with severe ID (M= 9.6 years were randomized into experimental and control groups. The experimental group participated in a semi-structured physical activity intervention, while the control group engaged in a supervised free play program, for 60 min per meeting, five times per week, over 12 months. Participants' FMS were assessed for pretest, 6-month midterm, and posttest; their age, body mass index (BMI) were also collected.

Outcomes And Results: Controlling for participants' BMI and pretest scores, the intervention significantly improved posttest FMS in the experimental group compared to the comparison group. No significant improvement was found from midterm to posttest for the comparison group. No significant improvement was found from pretest to midterm FMS performance for both groups.

Conclusions And Implications: The results suggest that the fundamental movement skill performance of children with severe ID can be improved, although the significant effects may only be evident after long (1 year), rather than short-term (6 months), time periods.
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http://dx.doi.org/10.1016/j.ridd.2021.103980DOI Listing
July 2021

Body Image Dissatisfaction and Impulse Buying: A Moderated Mediation Model.

Front Psychol 2021 26;12:653559. Epub 2021 Apr 26.

School of Psychology, Central China Normal University, Wuhan, China.

This study investigated the mediating roles of self-acceptance and self-esteem in the relationship between body image dissatisfaction and impulse buying, and tested the moderating effect of gender on the relationships. A sample of 374 college students and graduate students (33.4% male, 66.6% female) participated in the study. Results revealed that (a) body image dissatisfaction positively associated with impulse buying; (b) self-esteem plays a mediating role between body image dissatisfaction and impulsive buying; (c) a serial indirect pathway (i.e., Body image dissatisfaction → self-acceptance → self-esteem → impulse buying) emerged; (d) the mediation path from self-acceptance to self-esteem was stronger for female than that from male. The results underscore the importance of identifying the mechanisms that moderate the mediated path between body image dissatisfaction and impulse buying among students. These findings point to the potential implications about how to reduce impulse buying through improving body image satisfaction.
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http://dx.doi.org/10.3389/fpsyg.2021.653559DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107384PMC
April 2021

Genome-wide characterization of bZIP transcription factors and their expression patterns in response to drought and salinity stress in Jatropha curcas.

Int J Biol Macromol 2021 Jun 8;181:1207-1223. Epub 2021 May 8.

Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China. Electronic address:

The basic leucine zipper (bZIP) family is one of the largest families of transcription factors (TFs) in plants and is responsible for various functions, including regulating development and responses to abiotic/biotic stresses. However, the roles of bZIPs in the regulation of responses to drought stress and salinity stress remain poorly understood in Jatropha curcas L., a biodiesel crop. In the present study, 50 JcbZIP genes were identified and classified into ten groups. Cis-element analysis indicated that JcbZIP genes are associated with abiotic stress. Gene expression patterns and quantitative real-time PCR (qRT-PCR) showed that four JcbZIP genes (JcbZIPs 34, 36, 49 and 50) are key resistance-related genes under both drought and salinity stress conditions. On the basis of the results of cis-element and phylogenetic analyses, JcbZIP49 and JcbZIP50 are likely involved in responses to drought and salinity stress; moreover, JcbZIP34 and JcbZIP36 might also play important roles in seed development and response to abiotic stress. These findings advance our understanding of the comprehensive characteristics of JcbZIP genes and provide new insights for functional validation in the further.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.05.027DOI Listing
June 2021
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