Publications by authors named "Dandan Li"

936 Publications

The role of probiotics in vaginal health.

Front Cell Infect Microbiol 2022 28;12:963868. Epub 2022 Jul 28.

University of Chinese Academy of Sciences, Beijing, China.

Probiotics have been widely used in the treatment of intestinal diseases, but the effect of probiotics on female reproductive tract health is still controversial. is the most abundant microorganism in the vagina, which is related to the vaginal mucosal barrier. adheres to the vaginal epithelium and can competitively antagonize the colonization of pathogens. The factors produced by , such as bacteriocin and hydrogen peroxide (HO), can inhibit the growth of pathogenic microorganisms and maintain the low pH environment of the vagina. Probiotics play an important role in maintaining the stability of vaginal microenvironment, improving immune defense and blocking the progression of cervical cancer. We review the research progress of probiotics represented by in gynecological diseases such as human papilloma virus (HPV) infection, bacterial vaginosis (BV) and Genitourinary Syndrome of Menopause (GSM), so as to provide basis for further exerting the role of probiotics in women's health.
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http://dx.doi.org/10.3389/fcimb.2022.963868DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9366906PMC
August 2022

Prevalence of inappropriate use behaviors of antibiotics and related factors among chinese antibiotic users: an online cross-sectional survey.

BMC Infect Dis 2022 Aug 13;22(1):689. Epub 2022 Aug 13.

School of Health Services Management, Anhui Medical University, Hefei, 230032, People's Republic of China.

Background: Inappropriate use of antibiotics could have a profound negative impact on individual and community. This study aimed to assess the prevalence of inappropriate use behaviors of antibiotics in Chinese antibiotic users and explored their related factors.

Methods: A cross-sectional survey was conducted from July 1, 2018 to September 30, 2018. A structured questionnaire was widely distributed on the online survey platform (Questionnaire Star, https://www.wjx.cn ) and was used to collect data from respondents in China on demographic and sociological characteristics, antibiotic use and related knowledge. Main outcomes included self-medication with antibiotics (SMA), self-storage with antibiotics (SSA), and non-adherence to antibiotic treatment (NAAT). Logistic regression was used to identify the related factors of above inappropriate use behaviors of antibiotics.

Results: Of the 15,526 participants, 37.1% reported SMA in the past 6 months, 67.9% reported SSA in the past 6 months, and 48.3%, 15.2%, 25.5% and 78.0% of respondents said that they had missed antibiotics, increased antibiotic dosage, decreased antibiotic dosage, and discontinued antibiotics once symptoms disappear, respectively. Overall, 53.3% reported NAAT during this period. After adjusting for other variables, multivariate logistic regression analyses showed that those aged 30-44 years old, with higher levels of education, poorer status of self-perceived health, or lower levels of antibiotic knowledge were more likely to have these inappropriate use behaviors of SMA, SSA, and NAAT (P < 0.05).

Conclusions: The prevalence of SMA, SSA, and NAAT is high in China. Developing a nationwide action plan for the rational use of antibiotics among publics, including national media publicity, online and offline health education, and medication guidance from general practitioners, is urgently needed.
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http://dx.doi.org/10.1186/s12879-022-07671-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9375431PMC
August 2022

Hofmeister effect-enhanced gelatin/oxidized dextran hydrogels with improved mechanical properties and biocompatibility for wound healing.

Acta Biomater 2022 Aug 9. Epub 2022 Aug 9.

Department of Orthopedics, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, 301 Yanchang Road, Shanghai 200072, China. Electronic address:

Compared with other types of hydrogels, natural derived hydrogels possess intrinsic advantages of degradability and biocompatibility. However, due to the low mechanical strength, their potential applications in biomedical areas are limited. In this study, Hofmeister effect-enhanced gelatin/oxidized dextran (Gel/O-Dex) hydrogels were designed with improved mechanical properties and biocompatibility to accelerate wound healing. Gel and O-Dex were chemically crosslinked through Schiff base reaction of aldehyde and amino groups. After soaking in kosmotrope solutions physical crosslinking domains were induced by Hofmeister effect including α-helix structures, hydrophobic interaction regions and helical junction zones among Gel molecular chains. The type of anions played different influence on the properties of hydrogels, which was consistent with the order of Hofmeister series. Particularly, HPO treated hydrogels showed enhanced mechanical strength and fatigue resistance superior to that of Gel/O-Dex hydrogels. The underlying mechanism was that the physical crosslinking domains sustained additional mechanical stress and dissipated energy through cyclic association and dissociation process. Furthermore, Hofmeister effect only induced polymer chain entanglements without triggering any chemical reaction. Due to Hofmeister effect of HPO ions, aldehyde groups were embedded in the center of entangled polymer chains that resulted in better biocompatibility. In the full-thickness skin defects of SD rats, Hofmeister effect-enhanced Gel/O-Dex hydrogels by HPO ions accelerated wound healing and exhibited better histological morphology than ordinary hydrogels. Therefore, Hofmeister effect by essential inorganic anions is a promising method of improving mechanical properties and biocompatibility of natural hydrogels to promote medical translation in the field of wound healing from bench to clinic. STATEMENT OF SIGNIFICANCE: Hofmeister effect-enhanced hydrogel mechanical properties, in accordance with the order of Hofmeister series through physical crosslinking, induced α-helix structures, hydrophobic interaction regions and helical junction zones among Gel molecular chains. Due to the Hofmeister effect of HPO ions, aldehyde groups were embedded in the center of entangled polymer chains that resulted in better biocompatibility. Hofmeister effect-enhanced Gel/O-Dex hydrogels through HPO ions accelerated wound healing and exhibited better histological morphology than ordinary hydrogels. Therefore, the Hofmeister effect achieved by essential inorganic anions is a promising method of improving mechanical properties and biocompatibility of natural hydrogels to promote medical translation in the field of wound healing from the bench to the clinic.
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http://dx.doi.org/10.1016/j.actbio.2022.08.009DOI Listing
August 2022

Wide occurrence of seven phthalate plasticizers and two typical microplastics in pig feed.

Chemosphere 2022 Aug 6:135847. Epub 2022 Aug 6.

MOE Key Laboratory on Pollution Processes and Environmental Criteria, College of Environmental Science and Engineering, Nankai University, Tianjin 300350, China. Electronic address:

Plastics are widely used as packaging and engineering materials in feed processing, which leads to the potential contamination of plasticizers and microplastics (MPs) in animal feeds. In this study, the concentrations of two typical MPs, i.e., polyethylene terephthalate (PET) and polycarbonate (PC), and seven phthalates (PAEs) as well as their corresponding monoester metabolites (mPAEs) in 45 pig feed samples in China were analyzed by mass spectrometers. Among PAEs, dibutyl phthalate (DBP) and bis(2-ethylhexyl) phthalate (DEHP) were detected in all samples, and DEHP showed the highest concentrations of 8.26-2464 μg/kg, which accounted for 65.6% of the total detected PAEs. PET MPs (
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http://dx.doi.org/10.1016/j.chemosphere.2022.135847DOI Listing
August 2022

Multi-platform-based characterization of ferroptosis in human colorectal cancer.

iScience 2022 Aug 15;25(8):104750. Epub 2022 Jul 15.

Department of Clinical Medical Research Center, The Second Clinical Medical College, Jinan University (Shenzhen People's Hospital), Shenzhen 518020, China.

Ferroptosis is a type of programmed cell death potentially playing an important role in colorectal cancer (CRC) development. However, comprehensive investigations toward ferroptosis in human CRC are lacking. Here, we performed multiple investigations on cancer and para-cancer tissues. We demonstrated that the changes of structural variation and chromatin accessibility in CRC were more associated with the altered mRNA expression of ferroptosis-related genes (FRGs), and the expression of , , , and was related to the overall survival rates. Subsequently, we revealed that and were potentially the hub genes, and that and were potentially FRGs' upstream transcription factors. Finally, we depicted the crosstalk between ferroptosis and necrosis, autophagy, and apoptosis. Based on multi-dimensional analyses, we characterized ferroptosis, probable core genes, and the upstream regulators in human CRC. The findings here may improve our understanding of ferroptosis in CRC and provide new opportunities for clinical diagnosis and treatment.
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http://dx.doi.org/10.1016/j.isci.2022.104750DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9356096PMC
August 2022

Proteomics analysis of lysine crotonylation and 2-hydroxyisobutyrylation reveals significant features of systemic lupus erythematosus.

Clin Rheumatol 2022 Aug 8. Epub 2022 Aug 8.

Clinical Medical Research Center, the Second Clinical Medical College of Jinan University, Shenzhen People's Hospital, Shenzhen, 518020, Guangdong, China.

Introduction/objectives: To seek significant features of systemic lupus erythematosus (SLE) by utilizing bioinformatics analysis.

Method: Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to quantify lysine crotonylation (Kcr) and lysine 2-hydroxyisobutyrylation (Khib) in peripheral blood mononuclear cells (PBMCs) of systemic lupus erythematosus (SLE) patients and normal controls.

Results: Seventy-six differentially modified proteins (DMPs) dually modified by Kcr and Khib were identified between SLE patients and healthy people. GO enrichment analysis prompted significant enrichment of seventy-six DMPs in MHC class II protein complex binding and leukocyte migration. KEGG pathways were enriched in antigen processing and presentation pathway and leukocyte transendothelial migration pathway. Six DMPs (CLTC, HSPA1B, HSPA8, HSP90AB1, HSPD1, and PDIA3) were identified in antigen processing and presentation pathway, of which HSPA8 was the core protein. Significant changes of Kcr and Khib in HSPA8 may increase ATP hydrolysis and promote antigen binding to MHC II molecule. In leukocyte transendothelial migration pathway, 7 DMPs (ACTN1, ACTN4, EZR, MSN, RAC1, RHOA, and VCL) were identified. MSN was the protein with the most modification sites in this pathway. In amino terminal ferm region of MSN, Kcr and Khib expression change may lead to the adhesion between leukocytes and endothelial cells, which was an important step of leukocyte migration.

Conclusion: Kcr and Khib may promote the antigen presentation and jointly regulate the tissue damage mediated by leukocyte migration in SLE patients, which may play key roles in the pathogenesis of SLE probably. Key Points • Antigen processing and presentation and leukocyte transendothelial migration may play key roles in the pathogenesis of SLE.
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http://dx.doi.org/10.1007/s10067-022-06254-4DOI Listing
August 2022

Deep learning predicts resistance to neoadjuvant chemotherapy for locally advanced gastric cancer: a multicenter study.

Gastric Cancer 2022 Aug 6. Epub 2022 Aug 6.

Department of Radiology, Shanxi Province Cancer Hospital, Shanxi Hospital Affiliated to Cancer Hospital, Chinese Academy of Medical Sciences, Cancer Hospital, Affiliated to Shanxi Medical University, Taiyuan, 030013, Shanxi, China.

Background: Accurate pre-treatment prediction of neoadjuvant chemotherapy (NACT) resistance in patients with locally advanced gastric cancer (LAGC) is essential for timely surgeries and optimized treatments. We aim to evaluate the effectiveness of deep learning (DL) on computed tomography (CT) images in predicting NACT resistance in LAGC patients.

Methods: A total of 633 LAGC patients receiving NACT from three hospitals were included in this retrospective study. The training and internal validation cohorts were randomly selected from center 1, comprising 242 and 104 patients, respectively. The external validation cohort 1 comprised 128 patients from center 2, and the external validation cohort 2 comprised 159 patients from center 3. First, a DL model was developed using ResNet-50 to predict NACT resistance in LAGC patients, and the gradient-weighted class activation mapping (Grad-CAM) was assessed for visualization. Then, an integrated model was constructed by combing the DL signature and clinical characteristics. Finally, the performance was tested in internal and external validation cohorts using area under the receiver operating characteristic (ROC) curves (AUC).

Results: The DL model achieved AUCs of 0.808 (95% CI 0.724-0.893), 0.755 (95% CI 0.660-0.850), and 0.752 (95% CI 0.678-0.825) in validation cohorts, respectively, which were higher than those of the clinical model. Furthermore, the integrated model performed significantly better than the clinical model (P < 0.05).

Conclusions: A CT-based model using DL showed promising performance for predicting NACT resistance in LAGC patients, which could provide valuable information in terms of individualized treatment.
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http://dx.doi.org/10.1007/s10120-022-01328-3DOI Listing
August 2022

Are all fibres created equal with respect to lipid lowering? Comparing the effect of viscous dietary fibre to non-viscous fibre from cereal sources: a systematic review and meta-analysis of randomised controlled trials.

Br J Nutr 2022 Aug 5:1-13. Epub 2022 Aug 5.

Clinical Nutrition and Risk Factor Modification Centre, St. Michael's Hospital, Unity Health, Toronto, ON, Canada.

Although compelling evidence from observational studies supports a positive association between consumption of cereal fibre and CVD risk reduction, randomised controlled trials (RCT) often target viscous fibre type as the prospective contributor to lipid lowering to reduce CVD risk. The objective of our study is to compare the lipids-lowering effects of viscous dietary fibre to non-viscous, cereal-type fibre in clinical studies. RCT that evaluated the effect of viscous dietary fibre compared with non-viscous, cereal fibre on LDL cholesterol and alternative lipid markers, with a duration of ≥ 3 weeks, in adults with or without hypercholesterolaemia were included. Medline, EMBASE, CINAHL and the Cochrane Central Register were searched through October 19, 2021. Data were extracted and assessed by two independent reviewers. The generic inverse variance method with random effects model was utilised to pool the data which were expressed as mean differences (MD) with 95 % CI. Eighty-nine trials met eligibility criteria ( 4755). MD for the effect of viscous dietary fibre compared with non-viscous cereal fibre were LDL cholesterol (MD = -0·26 mmol/l; 95 % CI: -0·30, -0·22 mmol/l; < 0·01), non-HDL cholesterol (MD = -0·33 mmol/l; 95 % CI: -0·39, -0·28 mmol/l; < 0·01) and Apo-B (MD = -0·04 g/l; 95 % CI: -0·06, -0·03 g/l; < 0·01). Viscous dietary fibre reduces LDL cholesterol and alternative lipid markers relative to the fibre from cereal sources, hence may be a preferred type of fibre-based dietary intervention targeting CVD risk reduction.
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http://dx.doi.org/10.1017/S0007114522002355DOI Listing
August 2022

Development and validation of a maternal anxiety for neonatal jaundice scale in China.

BMC Psychiatry 2022 08 4;22(1):526. Epub 2022 Aug 4.

Department of Social Medicine and Health Management, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, No.13 Hangkong Road, 430030, Wuhan, P. R. China.

Background: Maternal anxiety induced by neonatal jaundice has adverse effects on maternal and infant health, but there was no specific tool to identify the anxiety level of mothers. This study aims to develop a Maternal Anxiety for Neonatal Jaundice Scale (MANJS) and to validate it in the target population.

Methods: An initial 11-items MANJS was developed through literature review, expert panel consultation, and a pilot-test. Subsequently, mothers of neonates with jaundice were recruited from the Maternal and Child Health Hospital of Hainan Province, China, from June to December 2018, for a formal questionnaire survey. Based on the data collected, the scale was validated for construct validity, convergent validity, discriminant validity, content validity, and internal consistency reliability after the items screening.

Results: The reliability and validity of MANJS were validated in 1127 mothers of jaundiced neonates. After the item with cross-loadings was removed using exploratory factor analysis, MANJS consisted of two dimensions and 10 items, with a cumulative variance contribution of 74.36% and factor loadings above 0.6 for all items. The confirmatory factor analysis identified three items with cross-factor loading or error correlation and then they were removed orderly. The further confirmatory factor analysis showed a good construct validity for the 7-item MANJS, with standardized root mean square residual (SRMR) = 0.029, root mean square error of approximation (RMSEA) = 0.068, comparative fit index (CFI) = 0.961, Tucker-Lewis index (TLI) = 0.937, incremental fit index (IFI) = 0.961, normed fit index (NFI) = 0.954, goodness of fit index (GFI) = 0.998, adjusted goodness of fit index (AGFI) = 0.996, respectively. The average variance extracted values (AVE) of the two factors were 0.80 and 0.72, and the combined reliability (CR) were 0.94 and 0.88, respectively. Cronbach's alpha was 0.90 for the MANJS, and split-half reliability was 0.72.

Conclusions: MANJS was demonstrated to have satisfactory reliability and validity in evaluating maternal anxiety caused by neonatal jaundice among Chinese postpartum women.
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http://dx.doi.org/10.1186/s12888-022-04161-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9351162PMC
August 2022

CRISPR/Cas9-mediated epigenetic editing tool: An optimized strategy for targeting de novo DNA methylation with stable status via homology directed repair pathway.

Biochimie 2022 Jul 31. Epub 2022 Jul 31.

National Center for Clinical Laboratories, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing Hospital/National Center of Gerontology, PR China; Graduate School of Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, PR China; Beijing Engineering Research Center of Laboratory Medicine, Beijing Hospital, Beijing, PR China. Electronic address:

With the development of epigenetic engineering tools for manipulating DNA methylation at the desired locus, the stable maintenance of targeted methylation status in edited cells after division poses a major obstacle. Transient methylation levels, along with the reversible inhibition of the desired genes, by some epigenetic editing techniques, limit their further application in functional and therapeutic studies of critical regulators in the cancer epigenome. In this study, we utilized CRISPR/Cas9-mediated the homology-directed repair (HDR) pathway following double-strand breaks (DSBs) information to target de novo methylation of the vital epigenetic biomarker, O-methylguanine-DNA methyltransferase (MGMT). Based on synthetic single and double stranded methylated repair templates, DNA methylation patterns can be incorporated into the endogenous MGMT gene. In addition, we observed upregulation of DNA methyltransferases (DNMTs) in edited HeLa cells. In particular, we employed an optimized method of using the DNA ligase IV inhibitor SCR7 to markedly enhance the insertion efficiency induced by the long methylated repair template, contributing to the induction of stable methylation alterations through epigenetic recombination after cell division. Additionally, our study confirmed that CRISPR/Cas9-mediated target methylation in a site-specific manner enabled the maintenance of gene silencing in vitro and in vivo. Collectively, we show that a combination of CRISPR/Cas9 components, SCR7 treatment, and the long methylated HDR template can enhance CRISPR/Cas9-directed epigenomic editing efficiency and further induce stable effects on methylation modifications and transcriptional suppression.
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http://dx.doi.org/10.1016/j.biochi.2022.07.013DOI Listing
July 2022

Partial Linker Substitution Strategy to Construct a Quaternary HKUST-like MOF for Efficient Acetylene Storage and Separation.

ACS Appl Mater Interfaces 2022 Aug 3;14(32):36882-36889. Epub 2022 Aug 3.

Fujian Provincial Key Laboratory of Polymer Materials, College of Chemistry and Materials Science, Fujian Normal University, Fuzhou 350007, P. R. China.

Multicomponent metal-organic frameworks (MOFs) have received much attention as emerging materials capable of precisely programing exquisite structures and specific functions. Here, we applied a partial linker substitution strategy to compile an HKUST-1-like quaternary MOF by introducing a bifunctional ligand into the well-known HKUST-1 structure. , a new HKUST-like topology MOF, was assembled with paddlewheel [Cu(COO)], triangular metallocycle pyrazole cluster Cu(μ-OH) (NN) building blocks, and two distinct linkers. exhibited good mechanical stability, water stability, and chemical stability (pH = 3-12) in aqueous solutions. Moreover, the porous environments created by this multicomponent primitive endow with high CH storage and significantly selective separation performance of CH/CO. Dynamic breakthrough experiments and ideal adsorbed solution theory calculations further demonstrate that can selectively capture CH from CH/CO mixtures under ambient conditions. Based on grand canonical Monte Carlo simulations, the high CH separation performance of is attributed to the π-complex formed between the CH molecule and the trinuclear metallocycle clusters on the wall, which provides stronger affinity for CH recognition than the CO molecule.
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http://dx.doi.org/10.1021/acsami.2c10346DOI Listing
August 2022

Targeting FHL1 impairs cell proliferation and differentiation of acute myeloid leukemia cells.

Biochem Cell Biol 2022 Aug 2. Epub 2022 Aug 2.

Department of Hematology, the First Affiliated Hospital of Harbin Medical University, No. 23, Youzheng Street, Nangang District, Harbin, Heilongjiang, China.

The four and a half LIM domains 1 (FHL1) is considered to play important roles in tumors. This study aims to investigate the role and precise mechanisms of FHL1 in acute myeloid leukemia (AML). Here, we found that FHL1 was highly expressed in AML. CCK8, flow cytometry, and Western blot analysis of cell cycle-related proteins showed that overexpression of FHL1 promoted proliferation and accelerated cell cycle progression in HL-60 cells. Conversely, knockdown of FHL1 inhibited the proliferation and induced cell cycle arrest in KG-1 cells. Furthermore, knockdown of FHL1 promoted cell differentiation, while overexpression of FHL1 restrained all-trans retinoic acid induced cell differentiation in HL-60 cells, revealed by Wright-Giemsa staining and cell surface antigen analysis. Moreover, in vivo experiments revealed that depletion of FHL1 inhibited tumor growth and led to increased levels of CD11b and CD14. Here, we first identify an unexpected and important role of FHL1 that contributes to the AML progression, indicating that FHL1 may be a potential therapeutic target for AML.
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http://dx.doi.org/10.1139/bcb-2021-0507DOI Listing
August 2022

Maximum surgical blood order schedule for flap reconstruction in oral and maxillofacial cancer patients.

BMC Oral Health 2022 08 1;22(1):322. Epub 2022 Aug 1.

Department of Oral and Maxillofacial Surgery, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, 107th Yanjiang Xi Road, Guangzhou, 510120, Guangdong, China.

Background: We established a MSBOS for flap reconstruction in oral and maxillofacial cancer patients.

Method: We enrolled 2080 cases of oral and maxillofacial flap reconstruction from January 1, 2010 to December 31, 2021. Patient data were collected, including age, sex, BMI, preoperative Hb levels, ASA grade, T stage, flap type, tumor location, and bone flap. Scoring criteria were established based on a multivariate model of independent risk variables and their odds ratios. Two flap-type groups were divided into low-risk, intermediate-risk and high-risk groups by the scoring criteria, and analyzed using univariate and multivariate logistic regression. Perioperative transfusion analysis identified independent risk factors at various Hb levels. The cumulative percentage of patients requiring perioperative blood transfusion for each surgical procedure was calculated to establish the MSBOS.

Results: (1) Regression analysis showed that BMI, tumor T staging, ASA grade, preoperative Hb level (male: Hb < 130 g/L, female: Hb < 120 g/L), and bone flap were independent risk factors for perioperative blood transfusion. (2) Regression analysis showed that independent risk factors for perioperative transfusion included the following: BMI, tumor T3-T4 stage, ASA III, IV grade, and free flap/pediculated flap/bone flap in patients with different Hb levels; T3-T4 stage, ASA grade III-IV in mildly anemic patients; and ASA grade III-IV in moderately anemic patients. (3) A MSBOS was established for flap reconstruction in head and neck cancer patients.

Conclusion: A MSBOS for head and neck cancer procedures was reduced by approximately 30% perioperative blood preparation while ensuring that clinical blood use standards were met. It help optimize blood inventory, and save blood resources.
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http://dx.doi.org/10.1186/s12903-022-02357-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9341105PMC
August 2022

Characterization of a novel chitinolytic Serratia marcescens strain TC-1 with broad insecticidal spectrum.

AMB Express 2022 Jul 30;12(1):100. Epub 2022 Jul 30.

School of Life Science and Agricultural Engineering, Nanyang Normal University, Nanyang, 473061, China.

The Gram-negative rod-shaped bacterium Serratia marcescens is an opportunistic pathogen of many organisms, including insects. We report the identification and optimal in vitro chitinase production conditions of a novel chitinolytic S. marcescens strain TC-1 isolated from a naturally infected white grub (Anomala corpulenta) collected from a peanut field at Nanyang city, Henan province, China. Strain identification was conducted by morphological, physiological, biochemical and molecular analyses. The amplified 16S rRNA gene of TC-1 showed a similarity greater than 99% with multiple strains of S. marcescens. Based on Neighbor-joining phylogenetic tree analysis of bacterial 16S rRNA gene sequences, TC-1 formed a clade with S. marcescens, clearly separated from other Serratia spp. The strain TC-1 showed larvicidal activities against five insect species (A. corpulenta, Plutella xylostella, Spodoptera exigua, Helicoverpa armigera, Bombyx mori) and the nematode Caenorhabditis elegans, but not against S. litura. The operating parameters of chitinase production by TC-1 were optimized by response surface methodology using a three-factor, three-level Box-Behnken experimental design. The effects of three independent variables i.e. colloidal chitin concentration (7-13 g l), incubation time (24-72 h) and incubation temperature (24-32 °C) on chitinase production by TC-1 were investigated. A regression model was proposed to correlate the independent variables for an optimal chitinase activity predicted as 20.946 U ml, using a combination of colloidal chitin concentration, incubation time and incubation temperature of 9.06 g l, 63.83 h and 28.12 °C, respectively. The latter agreed well with a mean chitinase activity of 20.761 ± 0.102 U ml measured in the culture supernatants of TC-1 grown under similar conditions with a colloidal chitin concentration, incubation time and incubation temperature of 9 g l, 64 h and 28 °C, respectively. Our study revealed the S. marcescens strain TC-1 with potential as a biocontrol agent of insect pests and nematodes and demonstrated the proposed regression model's potential to guide chitinase production by this strain.
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http://dx.doi.org/10.1186/s13568-022-01442-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9339060PMC
July 2022

A Bibliometric Analysis of Research on the Links Between Gut Microbiota and Atherosclerosis.

Front Cardiovasc Med 2022 12;9:941607. Epub 2022 Jul 12.

Institute of Geriatric, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, China.

Background: Emerging evidence has linked gut microbiota (GM) and its related metabolites to atherosclerosis (AS). This study aimed to analyze the evolution of GM in AS in the past decades, and provide valuable insights in this field.

Methods: Web of Science Core Collection (WoSCC) was applied to retrieve the publications related to GM in AS from their inception until 2 December 2021, and the data was analyzed in Microsoft Excel, Scimago Graphica, CiteSpace, and VOSviewer.

Results: In total, 560 documents were extracted from the WoSCC databases. The publications have shown rapid growth since 2008. China and Cleveland Clin were the most prolific country and institution, respectively. The journal with the most publications is , and was the most co-cited journal. Among 3556 related authors, Hazen, Stanley L., Tang, W. H. Wilson, and Wang, Zeneng were the top 3 contributing authors in this field. Aside from "gut microbiota," "atherosclerosis," the terms "TMAO," "metabolite," "obesity," and "phosphatidylcholine" were frequently occurred in the abstract and title of articles. Burst detection of keywords indicated that "metabolic syndrome," "acid," and "bile acid" were hot topics in recent years. According to the co-citation analysis of references, the research focus in this area has changed over time, and recent researches focus on choline, hypertension, butyrate, and berberine.

Conclusion: Our study showed that the researches of GM in AS have been flourishing, and the content themes were constantly deepened. Human GM is critical to atherosclerotic diseases, and this hot topic is still worthy of more focus in the future.
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http://dx.doi.org/10.3389/fcvm.2022.941607DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9314574PMC
July 2022

Reprogramming of Human B Cells from Secreting IgG to IgM by Genome Editing.

CRISPR J 2022 Jul 27. Epub 2022 Jul 27.

National Center for Clinical Laboratories, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing Hospital/National Center of Gerontology, PR China.

B lymphocytes are activated and regulated by their interactions with T cells, a process that results in one-way class switching of immunoglobulins (ig) from IgM to IgG, IgE, or IgA. In this study, we show the application of clustered regularly interspaced short palindromic repeat-Cas9-induced nonhomologous end joining in B cells to achieve reverse-directional Ig class switching. By electroporating Cas9 and guide RNA and a Cμ encoding donor into cells, we engineered IgG-secreting human B cell lines to switch to express IgM antibody. This approach offers a new potential path for the production of IgM antibodies.
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http://dx.doi.org/10.1089/crispr.2021.0093DOI Listing
July 2022

Effects of Land Use on the Soil Microbial Community in the Songnen Grassland of Northeast China.

Front Microbiol 2022 8;13:865184. Epub 2022 Jul 8.

Department of Animal Science and Technology, Northeast Agricultural University, Harbin, China.

Land use change obviously changes the plant community composition and soil properties of grasslands and thus affects multiple functions and services of grassland ecosystems. However, the response mechanisms of soil microorganisms, key drivers of the nutrient cycle and other soil functions during changes in grassland use type and associated vegetation are not well understood. In this study, Illumina high-throughput sequencing was used to analyze the changes in the soil microbial community structure of four grassland use types: exclosure (EL), mowed land (ML), grazed land (GL), and farmland (FL) in the Songnen Plain of Northeast China. The results showed that the FL and EL had significantly higher soil total nitrogen (TN) and lower soil electrical conductivity (EC) and pH than GL and ML. In contrast, the GL and ML had higher soil bulk density (BD) and organic matter, respectively, than the other land use types. In addition, the values of the Shannon diversity and Pielou's evenness indexes were highest in the EL of all the land use types. Based on the high-throughput sequencing results, we observed high levels of α diversity in the FL for both bacteria and fungi. A structural equation model (SEM) revealed that pH and EC had a direct and positive effect on the bacterial community structure and composition. In addition, plant taxonomic diversity (according to the Shannon diversity and Pielou's evenness indexes) indirectly affected the bacterial community composition via soil pH and EC. Notably, fungal composition was directly and positively correlated with soil nutrients and the value of Pielou's evenness index changed with land use type. In conclusion, soil properties and/or plant diversity might drive the changes in the soil microbial community structure and composition in different grassland use types.
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http://dx.doi.org/10.3389/fmicb.2022.865184DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9307977PMC
July 2022

Correlation Analysis Between Required Surgical Indexes and Complications in Patients With Coronary Heart Disease.

Front Surg 2022 6;9:948666. Epub 2022 Jul 6.

Department of Nursing, Hunan Provincial People's Hospital (The First-Affiliated Hospital of Hunan Normal University), Changsha, China.

A total of 215 patients with coronary heart disease (CHD) were analyzed with SPSS. Samples of different genders showed significance in the obtuse marginal branch of the left circumflex branch × 1, the diagonal branch D1 × 1, and the ms PV representation. Patients with left circumflex branch occlusion are more male and tend to be younger. Age displayed a positive correlation with left intima-media thickness (IMT) and right IMT. This indicated that as age increases, the values of left IMT and right IMT increase. Samples of different CHD types showed significance in the obtuse marginal branch of the left circumflex branch × 1, the middle part of RCA × 1, and the middle part of the left anterior descending branch × 1.5. For non-ST-segment elevation angina pectoris with acute total vascular occlusion, the left circumflex artery is the most common, followed by the right coronary artery and anterior descending branch. Ultrasound of carotid IMT in patients with CHD can predict changes in left ventricular function, but no specific correlation between left and right common carotid IMT was found. Samples with or without the medical history of ASCVD showed significance in the branch number of coronary vessel lesions. The value of the branch number of coronary vessel lesions in patients with atherosclerotic cardiovascular disease (ASCVD) was higher than in those without ASCVD. The occurrence of complication is significantly relative with the distance of left circumflex branch × 1, the middle segment of left anterior descending branch × 1.5, and the distance of left anterior descending branch × 1. For patients without complications, the values in the distal left circumflex branch × 1, the middle left anterior descending branch × 1.5, and the distal left anterior descending branch × 1 were higher than those for patients with complications. The VTE scores showed a positive correlation with the proximal part of RCA × 1, the branch number of coronary vessel lesions, the posterior descending branch of left circumflex branch × 1, the distal part of left circumflex branch × 1, and the middle part of left anterior descending branch × 1.5.
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http://dx.doi.org/10.3389/fsurg.2022.948666DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9299069PMC
July 2022

Characterization of the Immunologic Phenotype of Dendritic Cells Infected With Herpes Simplex Virus 1.

Front Immunol 2022 5;13:931740. Epub 2022 Jul 5.

Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, China.

Due to viral envelope glycoprotein D binding to cellular membrane HVEM receptor, HSV-1 can infect certain dendritic cells, which becomes an event in the viral strategy to interfere with the host's immune system. We previously generated the HSV-1 mutant strain M6, which produced an attenuated phenotype in mice and rhesus monkeys. The attenuated M6 strain was used to investigate how HSV-1 infection of dendritic cells interferes with both innate and adaptive immunity. Our study showed that dendritic cells membrane HVEM receptors could mediate infection of the wild-type strain and attenuated M6 strain and that dendritic cells infected by both viruses in local tissues of animals exhibited changes in transcriptional profiles associated with innate immune and inflammatory responses. The infection of pDCs and cDCs by the two strains promoted cell differentiation to the CD103 phenotype, but varied transcriptional profiles were observed, implying a strategy that the HSV-1 wild-type strain interferes with antiviral immunity, probably due to viral modification of the immunological phenotype of dendritic cells during processing and presentation of antigen to T cells, leading to a series of deviations in immune responses, ultimately generating the deficient immune phenotype observed in infected individuals in the clinical.
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http://dx.doi.org/10.3389/fimmu.2022.931740DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9294641PMC
July 2022

DNA hypermethylation promotes the flowering of orchardgrass during vernalization.

Plant Physiol 2022 Jul 21. Epub 2022 Jul 21.

College of Grassland Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.

Vernalization, influenced by environmental factors, is an essential process associated with the productivity of temperate crops, during which epigenetic regulation of gene expression plays an important role. Although DNA methylation is one of the major epigenetic mechanisms associated with the control of gene expression, global changes in DNA methylation in the regulation of gene expression during vernalization-induced flowering of temperate plants remain largely undetermined. To characterize vernalization-associated DNA methylation dynamics, we performed whole-genome bisulphite-treated sequencing and transcriptome sequencing in orchardgrass (Dactylis glomerata) during vernalization. The results revealed that increased levels of genome DNA methylation during early vernalization of orchardgrass were associated with transcriptional changes in DNA methyltransferase and demethylase genes. Up-regulated expression of vernalization-related genes during early vernalization was attributable to an increase in mCHH in the promoter regions of these genes. Application of an exogenous DNA methylation accelerator or overexpression of orchardgrass NUCLEAR POLY(A) POLYMERASE (DgPAPS4) promoted earlier flowering, indicating that DNA hypermethylation plays an important role in vernalization-induced flowering. Collectively, our findings revealed that vernalization-induced hypermethylation is responsible for floral primordium initiation and development. These observations provide a theoretical foundation for further studies on the molecular mechanisms underlying the control of vernalization in temperate grasses.
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http://dx.doi.org/10.1093/plphys/kiac335DOI Listing
July 2022

Transanal full-thickness pull-through approach in the treatment of anastomotic leakage after operation for Hirschsprung disease.

Pediatr Surg Int 2022 Sep 19;38(9):1263-1271. Epub 2022 Jul 19.

Department of Pediatric Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Ave, Wuhan, 430030, China.

Background: Anastomotic leakage (AL) is one of the most perplexing complications that can occur following a radical operation to treat Hirschsprung disease (HSCR). The purpose of this study was to document our experience with anastomotic leakage following radical HSCR surgery to enhance therapeutic effect and prognosis.

Methods: Between January 2007 and April 2021, a retrospective study was conducted on 12 children who developed anastomotic leakage following radical surgery for HSCR. Medical records were analyzed to determine the clinical manifestations, primary surgical procedures, evaluation methods, surgical plans, and outcomes of the patients. To assess postoperative bowel function, the Rintala score was used.

Results: The Soave procedure was used as the primary surgical method in seven cases (58.3%), the Swenson procedure was used in four cases (33.3%), and the Rehbein procedure was used in one case (8.3%). Enterostomy (10, 83.3%) and conservative treatment (2, 16.7%) were performed when anastomotic leakage was diagnosed. Two patients who directly closed stoma without redoing pull-through both accepted enterostomy within 48 h. One female with anastomotic fistula who was closed leakage or fistula in situ had to endure lifelong stoma. Other patients who underwent redo pull-through procedures had normal bowel function. Seven patients underwent a redo pull-through procedure. Three of them preferred the transanal full-thickness pull-through (FTPT) approach, while four preferred the Soave technique. Three children had mild postoperative soiling, which improved with conservative treatment. Bowel function score was 17.5 ± 1.1.

Conclusion: Enterostomy should be performed immediately if anastomotic leakage occurs. After leakage, it is necessary to redo the pull-through procedure in an anastomotic fistula or anastomotic stricture. Transanal FTPT reconstruction is an effective method for repairing anastomoses and leakage.
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http://dx.doi.org/10.1007/s00383-022-05164-5DOI Listing
September 2022

Modulation of the Fluorination Site on Side-Chain Thiophene Improved Efficiency in All-Small-Molecule Organic Solar Cells.

ACS Appl Mater Interfaces 2022 Jul 14. Epub 2022 Jul 14.

Zhejiang Engineering Research Center for Energy Optoelectronic Materials and Devices, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo 315201, China.

Fine-tuning the phase-separated morphology is of great importance to achieve efficient all-small-molecule organic solar cells (ASM-OSCs). In this work, a pair of isomers are designed and synthesized, namely, BDT-UF and BDT-DF, in which the fluorine atom in BDT-UF is close to the alkyl chain of side-chain thiophene, while that in BDT-DF is close to the center core. Owing to the noncovalent interaction between fluorine and hydrogen, BDT-DF shows a smaller dihedral angle between the thiophene side chain and the BDT core, which causes better molecular planarity. When mixed with N3, BDT-UF shows better miscibility, higher crystallinity, and more ordered molecule stacking in the blend film. Finally, the device of BDT-DF:N3 gains a power conversion efficiency (PCE) of 14.5%, while that of BDT-UF:N3 shows an increase in , , and FF and gains a PCE of 15.1%. Our work exhibits a way of adjusting the substitution site of fluorine atoms to improve the PCE of ASM-OSCs.
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http://dx.doi.org/10.1021/acsami.2c07791DOI Listing
July 2022

Methylation-blocked cascade strand displacement amplification for rapid and sensitive fluorescence detection of DNA methyltransferase activity.

J Pharm Biomed Anal 2022 Sep 7;219:114935. Epub 2022 Jul 7.

College of Chemistry and Bioengineering, Guilin University of Technology, Guangxi 541004, China. Electronic address:

DNA methylation catalyzed by DNA adenine methylation methyltransferase (Dam MTase) is strongly connected with a variety of biological processes, hence, monitoring Dam MTase activity is of great importance. Here, we developed a rapid and sensitive fluorescence sensing strategy for the detection of Dam MTase activity based on methylation-blocked enzymatic recycling amplification. In this fluorescence sensing system, Dam MTase-induced methylation blocked the subsequent reactions. In contrast, in the absence of Dam MTase, the unmethylated probe initiated the cascade strand displacement amplification for significant signal amplification. Under optimized conditions, this method has a lower detection limit of 0.67 U/mL and a shorter assay time (90 min) compared with previously reported similar methodologies.
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http://dx.doi.org/10.1016/j.jpba.2022.114935DOI Listing
September 2022

Inflammation accelerates BCR-ABL1+ B-ALL development through upregulation of AID.

Blood Adv 2022 07;6(13):4060-4072

Department of Pathogenic Biology and Immunology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an, China.

Inflammation contributes to the initiation and disease progression of several lymphoid malignancies. BCR-ABL1-positive B-cell acute lymphoblastic leukemia (BCR-ABL1+ B-ALL) is triggered by the malignant cloning of immature B cells promoted by the BCR-ABL1 fusion gene. However, it is unclear whether the mechanism driving the disease progression of BCR-ABL1+ B-ALL involves inflammatory stimulation. Here, we evaluate BCR-ABL1+ B-ALL cells' response to inflammatory stimuli lipopolysaccharide (LPS) in vitro and in vivo. The results indicate that LPS promotes cell growth and genomic instability in cultured BCR-ABL1+ B-ALL cells and accelerates the BCR-ABL1+ B-ALL development in a mouse model. We show that the LPS-induced upregulation of activation-induced deaminase (AID) is required for the cell growth and disease progression of BCR-ABL1+ B-ALL. Moreover, AID modulates the expression of various genes that are dominated by suppressing apoptosis genes and upregulating DNA damage-repair genes. These genes lead to facilitation for BCR-ABL1+ B-ALL progression. The heat shock protein 90 (Hsp90) inhibitors significantly reduce AID protein level and delay the disease progression of BCR-ABL1+ B-ALL upon inflammatory stimulation. The present data demonstrate the causative role of AID in the development and progression of BCR-ABL1+ B-ALL during inflammation, thus highlighting potential therapeutic targets.
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http://dx.doi.org/10.1182/bloodadvances.2021005017DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9278295PMC
July 2022

Characterization and Identification of Aptamers against CD49c for the Detection, Capture, and Release of Cancer Cells.

ACS Appl Bio Mater 2022 07 6;5(7):3461-3468. Epub 2022 Jul 6.

Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.

As a kind of recognition molecule, aptamers can be inserted into some regulatory sequences for the smart response of their targets. However, the molecular engineering might lead to the change of the binding affinity. Here, we present a stable aptamer ZAJ-2c and an environmentally sensitive aptamer ZAJ-2d optimized from an original cell-binding aptamer ZAJ-2, and the molecular target was further identified as CD49c on the cell membrane. ZAJ-2c was characterized with high binding ability independent of the presence of divalent cations at a temperature range from 4 to 37 °C, showing promise for measuring the expression of CD49c on cancer cells. Moreover, ZAJ-2d had a nanomolar binding affinity in the binding buffer at 4 °C, the same as ZAJ-2c, but lost the binding ability in a PBS buffer supplemented with 5 mM EDTA at 37 °C. This aptamer variant proved to selectively capture and release the CD49c positive cells by simply adjusting the temperatures and divalent cations. This set of aptamers might provide a toolbox for monitoring and operating of a wide range of cancer cells with CD49c expression on the surface, which will be helpful for the studying the heterogeneity of rare cells.
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http://dx.doi.org/10.1021/acsabm.2c00389DOI Listing
July 2022

Advances in the Application of Radionuclide-Labeled HER2 Affibody for the Diagnosis and Treatment of Ovarian Cancer.

Front Oncol 2022 15;12:917439. Epub 2022 Jun 15.

Department of Nuclear Medicine, Affiliated Hospital of Zunyi Medical University, Zunyi, China.

Human epidermal growth factor receptor 2 (HER2) is a highly expressed tumor marker in epithelial ovarian cancer, and its overexpression is considered to be a potential factor of poor prognosis. Therefore, monitoring the expression of HER2 receptor in tumor tissue provides favorable conditions for accurate localization, diagnosis, targeted therapy, and prognosis evaluation of cancer foci. Affibody has the advantages of high affinity, small molecular weight, and stable biochemical properties. The molecular probes of radionuclide-labeled HER2 affibody have recently shown broad application prospects in the diagnosis and treatment of ovarian cancer; the aim is to introduce radionuclides into the cancer foci, display systemic lesions, and kill tumor cells through the radioactivity of the radionuclides. This process seamlessly integrates the diagnosis and treatment of ovarian cancer. Current research and development of new molecular probes of radionuclide-labeled HER2 affibody should focus on overcoming the deficiencies of non-specific uptake in the kidney, bone marrow, liver, and gastrointestinal tract, and on reducing the background of the image to improve image quality. By modifying the amino acid sequence; changing the hydrophilicity, surface charge, and lipid solubility of the affibody molecule; and using different radionuclides, chelating agents, and labeling conditions to optimize the labeling method of molecular probes, the specific uptake of molecular probes at tumor sites will be improved, while reducing radioactive retention in non-target organs and obtaining the best target/non-target value. These measures will enable the clinical use of radionuclide-labeled HER2 affibody molecular probes as soon as possible, providing a new clinical path for tumor-specific diagnosis, targeted therapy, and efficacy evaluation. The purpose of this review is to describe the application of radionuclide-labeled HER2 affibody in the imaging and treatment of ovarian cancer, including its potential clinical value and dilemmas.
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http://dx.doi.org/10.3389/fonc.2022.917439DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9240272PMC
June 2022

Diversity and Biogeography of Human Oral Saliva Microbial Communities Revealed by the Earth Microbiome Project.

Front Microbiol 2022 13;13:931065. Epub 2022 Jun 13.

National Administration of Health Data, Jinan, China.

The oral cavity is an important window for microbial communication between the environment and the human body. The oral microbiome plays an important role in human health. However, compared to the gut microbiome, the oral microbiome has been poorly explored. Here, we analyzed 404 datasets from human oral saliva samples published by the Earth Microbiome Project (EMP) and compared them with 815 samples from the human gut, nose/pharynx, and skin. The diversity of the human saliva microbiome varied significantly among individuals, and the community compositions were complex and diverse. The saliva microbiome showed the lowest species diversity among the four environment types. Human oral habitats shared a small core bacterial community containing only 14 operational taxonomic units (OTUs) under 5 phyla, which occupied over 75% of the sequence abundance. For the four habitats, the core taxa of the saliva microbiome had the greatest impact on saliva habitats than other habitats and were mostly unique. In addition, the saliva microbiome showed significant differences in the populations of different regions, which may be determined by the living environment and lifestyle/dietary habits. Finally, the correlation analysis showed high similarity between the saliva microbiome and the microbiomes of Aerosol (non-saline) and Surface (non-saline), i.e., two environment types closely related to human, suggesting that contact and shared environment being the driving factors of microbial transmission. Together, these findings expand our understanding of human oral diversity and biogeography.
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http://dx.doi.org/10.3389/fmicb.2022.931065DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9234457PMC
June 2022

Association between GNAQ Gene DNA Methylation and Vascular Recurrence in Patients with Acute Ischemic Stroke or Transient Ischemic Attack.

Cerebrovasc Dis 2022 Jun 28:1-10. Epub 2022 Jun 28.

Department of Interventional Neuroradiology, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.

Purpose: We aimed to assess whether the aberrant methylation of GNAQ gene, which may involve in the clopidogrel resistance (CR), was associated with a higher risk of recurrent ischemic events in clopidogrel-treated acute ischemic stroke or transient ischemic attack (TIA) patients.

Methods: This is a nested case-control study, 152 clopidogrel-treated acute ischemic stroke or TIA patients that were propensity-matched were included in the final analysis, including 36 patients with vascular recurrence set as cases. Methylation levels of GNAQ gene were identified with MassARRAY EpiTYPER assays. Univariate and multivariate logistic regression analyses were conducted to explore the predictive value of CpG units for recurrent ischemic events within 1 year.Mediation analysis was performed to assess the role of CR in describing the effect of GNAQ methylation on recurrent ischemic events.

Results: A total of 16 differentially methylated CpG units were identified. Multivariate logistic analysis indicated that the average methylation of CpG 32-39 of GNAQ was associated with a significantly higher risk of ischemic events (p < 0.001). When transformed into dichotomous variables with the receiver operating characteristic curve, hypomethylation (<0.31) of CpG 32-39 of GNAQ significantly increased the risk of vascular recurrence (odds ratio 73.82, 95% confidence interval 20.33-268.01). The mediation effect of CR for recurrent ischemic events was not identified.

Conclusions: Hypomethylation of CpG 32-39 of GANQ gene was associated with a higher risk of ischemic events for clopidogrel-treated acute ischemic stroke or TIA patients. Further studies were warranted to explain the possible mechanism.
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http://dx.doi.org/10.1159/000524416DOI Listing
June 2022

Immunological Study of Combined Administration of SARS-CoV-2 DNA Vaccine and Inactivated Vaccine.

Vaccines (Basel) 2022 Jun 10;10(6). Epub 2022 Jun 10.

Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Diseases, Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming 650118, China.

Objective: We constructed two DNA vaccines containing the receptor-binding domain (RBD) genes of multiple SARS-CoV-2 variants and used them in combination with inactivated vaccines in a variety of different protocols to explore potential novel immunization strategies against SARS-CoV-2 variants.

Methods: Two DNA vaccine candidates with different signal peptides (namely, secreted and membrane signal peptides) and RBD protein genes of different SARS-CoV-2 strains (Wuhan-Hu-1, B.1.351, B.1.617.2, C.37) were used. Four different combinations of DNA and inactivated vaccines were tested, namely, Group A: three doses of DNA vaccine; B: three doses of DNA vaccine and one dose of inactivated vaccine; C: two doses of inactivated vaccine and one dose of DNA vaccine; and D: coadministration of DNA and inactivated vaccines in two doses. Subgroups were grouped according to the signal peptide used (subgroup 1 contained secreted signal peptides, and subgroup 2 contained membrane signal peptides). The in vitro expression of the DNA vaccines, the humoral and cellular immunity responses of the immunized mice, the immune cell population changes in local lymph nodes, and proinflammatory cytokine levels in serum samples were evaluated.

Results: The antibody responses and cellular immunity in Group A were weak for all SARS-CoV-2 strains; for Group B, there was a great enhancement of neutralizing antibody (Nab) titers against the B.1.617.2 variant strain. Group C showed a significant increase in antibody responses (NAb titers against the Wuhan-Hu-1 strain were 768 and 1154 for Group C1 and Group C2, respectively, versus 576) and cellular immune responses, especially for variant B.1.617.2 (3240 ( < 0.001) and 2430 ( < 0.05) for Group C1 and Group C2, versus 450); Group D showed an improvement in immunogenicity. Group C induced higher levels of multiple cytokines.

Conclusion: The DNA vaccine candidates we constructed, administered as boosters, could enhance the humoral and cellular immune responses of inactivated vaccines against COVID-19, especially for B.1.617.2.
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http://dx.doi.org/10.3390/vaccines10060929DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9228235PMC
June 2022

Frequency-Specific Analysis of the Dynamic Reconfiguration of the Brain in Patients with Schizophrenia.

Brain Sci 2022 Jun 1;12(6). Epub 2022 Jun 1.

College of Information and Computer, Taiyuan University of Technology, No. 209, Daxue Street, Yuci District, Jinzhong 030024, China.

The analysis of resting-state fMRI signals usually focuses on the low-frequency range/band (0.01-0.1 Hz), which does not cover all aspects of brain activity. Studies have shown that distinct frequency bands can capture unique fluctuations in brain activity, with high-frequency signals (>0.1 Hz) providing valuable information for the diagnosis of schizophrenia. We hypothesized that it is meaningful to study the dynamic reconfiguration of schizophrenia through different frequencies. Therefore, this study used resting-state functional magnetic resonance (RS-fMRI) data from 42 schizophrenia and 40 normal controls to investigate dynamic network reconfiguration in multiple frequency bands (0.01-0.25 Hz, 0.01-0.027 Hz, 0.027-0.073 Hz, 0.073-0.198 Hz, 0.198-0.25 Hz). Based on the time-varying dynamic network constructed for each frequency band, we compared the dynamic reconfiguration of schizophrenia and normal controls by calculating the recruitment and integration. The experimental results showed that the differences between schizophrenia and normal controls are observed in the full frequency, which is more significant in slow3. In addition, as visual network, attention network, and default mode network differ a lot from each other, they can show a high degree of connectivity, which indicates that the functional network of schizophrenia is affected by the abnormal brain state in these areas. These shreds of evidence provide a new perspective and promote the current understanding of the characteristics of dynamic brain networks in schizophrenia.
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http://dx.doi.org/10.3390/brainsci12060727DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9221032PMC
June 2022
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