Publications by authors named "Dan Yue"

68 Publications

Metronomic capecitabine as adjuvant therapy in locoregionally advanced nasopharyngeal carcinoma: a multicentre, open-label, parallel-group, randomised, controlled, phase 3 trial.

Lancet 2021 07 7;398(10297):303-313. Epub 2021 Jun 7.

Department of Radiation Oncology, Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangdong Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and Therapy, Guangzhou, China.

Background: Patients with locoregionally advanced nasopharyngeal carcinoma have a high risk of disease relapse, despite a high proportion of patients attaining complete clinical remission after receiving standard-of-care treatment (ie, definitive concurrent chemoradiotherapy with or without induction chemotherapy). Additional adjuvant therapies are needed to further reduce the risk of recurrence and death. However, the benefit of adjuvant chemotherapy for nasopharyngeal carcinoma remains controversial, highlighting the need for more effective adjuvant treatment options.

Methods: This multicentre, open-label, parallel-group, randomised, controlled, phase 3 trial was done at 14 hospitals in China. Patients (aged 18-65 years) with histologically confirmed, high-risk locoregionally advanced nasopharyngeal carcinoma (stage III-IVA, excluding T3-4N0 and T3N1 disease), no locoregional disease or distant metastasis after definitive chemoradiotherapy, an Eastern Cooperative Oncology Group performance status of 0 or 1, sufficient haematological, renal, and hepatic function, and who had received their final radiotherapy dose 12-16 weeks before randomisation, were randomly assigned (1:1) to receive either oral metronomic capecitabine (650 mg/m body surface area twice daily for 1 year; metronomic capecitabine group) or observation (standard therapy group). Randomisation was done with a computer-generated sequence (block size of four), stratified by trial centre and receipt of induction chemotherapy (yes or no). The primary endpoint was failure-free survival, defined as the time from randomisation to disease recurrence (distant metastasis or locoregional recurrence) or death due to any cause, in the intention-to-treat population. Safety was assessed in all patients who received at least one dose of capecitabine or who had commenced observation. This trial is registered with ClinicalTrials.gov, NCT02958111.

Findings: Between Jan 25, 2017, and Oct 25, 2018, 675 patients were screened, of whom 406 were enrolled and randomly assigned to the metronomic capecitabine group (n=204) or to the standard therapy group (n=202). After a median follow-up of 38 months (IQR 33-42), there were 29 (14%) events of recurrence or death in the metronomic capecitabine group and 53 (26%) events of recurrence or death in the standard therapy group. Failure-free survival at 3 years was significantly higher in the metronomic capecitabine group (85·3% [95% CI 80·4-90·6]) than in the standard therapy group (75·7% [69·9-81·9]), with a stratified hazard ratio of 0·50 (95% CI 0·32-0·79; p=0·0023). Grade 3 adverse events were reported in 35 (17%) of 201 patients in the metronomic capecitabine group and in 11 (6%) of 200 patients in the standard therapy group; hand-foot syndrome was the most common adverse event related to capecitabine (18 [9%] patients had grade 3 hand-foot syndrome). One (<1%) patient in the metronomic capecitabine group had grade 4 neutropenia. No treatment-related deaths were reported in either group.

Interpretation: The addition of metronomic adjuvant capecitabine to chemoradiotherapy significantly improved failure-free survival in patients with high-risk locoregionally advanced nasopharyngeal carcinoma, with a manageable safety profile. These results support a potential role for metronomic chemotherapy as an adjuvant therapy in the treatment of nasopharyngeal carcinoma.

Funding: The National Natural Science Foundation of China, the Key-Area Research and Development Program of Guangdong Province, the Natural Science Foundation of Guangdong Province, the Innovation Team Development Plan of the Ministry of Education, and the Overseas Expertise Introduction Project for Discipline Innovation.

Translation: For the Chinese translation of the abstract see Supplementary Materials section.
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http://dx.doi.org/10.1016/S0140-6736(21)01123-5DOI Listing
July 2021

Piston Error Measurement for Segmented Telescopes with an Artificial Neural Network.

Sensors (Basel) 2021 May 12;21(10). Epub 2021 May 12.

College of Science, Changchun University of Science and Technology, Changchun 130022, China.

A piston error detection method is proposed based on the broadband intensity distribution on the image plane using a back-propagation (BP) artificial neural network. By setting a mask with a sparse circular clear multi-subaperture configuration in the exit pupil plane of a segmented telescope to fragment the pupil, the relation between the piston error of segments and amplitude of the modulation transfer function (MTF) sidelobes is strictly derived according to the Fourier optics principle. Then the BP artificial neural network is utilized to establish the mapping relation between them, where the amplitudes of the MTF sidelobes directly calculated from theoretical relationship and the introduced piston errors are used as inputs and outputs respectively to train the network. With the well trained-network, the piston errors are measured to a good precision using one in-focused broadband image without defocus division as input, and the capture range achieving the coherence length of the broadband light is available. Adequate simulations demonstrate the effectiveness and accuracy of the proposed method; the results show that the trained network has high measurement accuracy, wide detection range, quite good noise immunity and generalization ability. This method provides a feasible and easily implemented way to measure piston error and can simultaneously detect the multiple piston errors of the entire aperture of the segmented telescope.
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http://dx.doi.org/10.3390/s21103364DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8151092PMC
May 2021

Erratum to "ILC2 Proliferated by IL-33 Stimulation Alleviates Acute Colitis in Rag1 Mouse through Promoting M2 Macrophage Polarization".

J Immunol Res 2021 11;2021:9843489. Epub 2021 May 11.

Department of Immunology, China Medical University, No. 77 Puhe Road, Shenyang North New Area, Shenyang, Liaoning Province, China.

[This corrects the article DOI: 10.1155/2020/5018975.].
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http://dx.doi.org/10.1155/2021/9843489DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8131135PMC
May 2021

Expression of G3BP1 in benign and malignant human prostate tissues.

Transl Androl Urol 2021 Apr;10(4):1665-1675

Department of Urology, The Second Hospital of Tianjin Medical University, Tianjin Institute of Urology and School of Medical Laboratory, Tianjin Medical University, Tianjin, China.

Background: Prostate cancer (PCa) is the world's leading type of cancer in men. GTPase-activating protein SH3 domain-binding protein 1 (G3BP1) is overexpressed in a variety of tumors. However, there are limited studies in PCa concerning G3BP1. This present study was to investigates the expression of G3BP1 and the mechanism of action on PCa.

Methods: We explored the G3BP1 expression in PCa using the TCGA database and verified it using clinical samples by immunohistochemistry (IHC) methods. G3BP1 and Androgen receptor (AR) status of 104 human PCa and 50 benign prostate hyperplasia (BPH) samples were analyzed by IHC and the association between G3BP1 expression and biochemical recurrence was determined. Moreover, we generated G3BP1 knockdown cell lines in human PCa LNCaP cell lines, to observe AR changes.

Results: G3BP1 and AR were overexpressed in PCa compared to BPH tissues. The expression of G3BP1 and AR was positively correlated with the malignant degree of the tumor. Higher G3BP1 expression showed a trend toward biochemical recurrence. Western blot showed downregulation of G3BP1 affected AR expression levels.

Conclusions: Our study suggested that G3BP1 was frequently upregulated in PCa and closely related to AR expression and tumor metastasis. Besides, G3BP1 might be associated with biochemical recurrence. These results supply potential target for the management of the PCa.
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http://dx.doi.org/10.21037/tau-20-1450DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8100838PMC
April 2021

The genus (Hymenoptera, Apoidea, Crabronidae) in China, with description of two new species.

Zookeys 2021 18;1025:21-34. Epub 2021 Mar 18.

Department of Entomology, College of Plant Protection, Yunnan Agricultural University, Kunming, Yunnan, 650201, China Yunnan Agricultural University Kunming China.

Two new species of the genus Lepeletier de Saint Fargeau & Brullé (Crabronidae, Crabroninae, Crabronini) from China are described and illustrated, namely Yue & Ma, from Yunnan, and Yue & Li, from Hainan. In addition, Leclercq and Leclercq are recorded for the first time from China. A key to the species of from China is provided.
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http://dx.doi.org/10.3897/zookeys.1025.59920DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7994288PMC
March 2021

Anthropomorphic Strategies Promote Wildlife Conservation through Empathy: The Moderation Role of the Public Epidemic Situation.

Int J Environ Res Public Health 2021 03 30;18(7). Epub 2021 Mar 30.

Key Laboratory of Behavioral Science, Institute of Psychology, Chinese Academy of Sciences, Beijing 100101, China.

The global illegal wildlife trade directly threatens biodiversity and leads to disease outbreaks and epidemics. In order to avoid the loss of endangered species and ensure public health security, it is necessary to intervene in illegal wildlife trade and promote public awareness of the need for wildlife conservation. Anthropomorphism is a basic and common psychological process in humans that plays a crucial role in determining how a person interacts with other non-human agents. Previous research indicates that anthropomorphizing nature entities through metaphors could increase individual behavioral intention of wildlife conservation. However, relatively little is known about the mechanism by which anthropomorphism influences behavioral intention and whether social context affects the effect of anthropomorphism. This research investigated the impact of negative emotions associated with a pandemic situation on the effectiveness of anthropomorphic strategies for wildlife conservation across two experimental studies. Experiment 1 recruited 245 college students online and asked them to read a combination of texts and pictures as anthropomorphic materials. The results indicated that anthropomorphic materials could increase participants' empathy and decrease their wildlife product consumption intention. Experiment 2 recruited 140 college students online and they were required to read the same materials as experiment 1 after watching a video related to epidemics. The results showed that the effect of wildlife anthropomorphization vanished if participants' negative emotion was aroused by the video. The present research provides experimental evidence that anthropomorphic strategies would be useful for boosting public support for wildlife conservation. However, policymakers and conservation organizations must be careful about the negative effects of the pandemic context, as the negative emotions produced by it seems to weaken the effectiveness of anthropomorphic strategies.
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http://dx.doi.org/10.3390/ijerph18073565DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8037496PMC
March 2021

The Effect of Residual Triton X-100 on Structural Stability and Infection Activity of Adenovirus Particles.

Mol Ther Methods Clin Dev 2020 Dec 20;19:35-46. Epub 2020 Aug 20.

State Key Laboratory of Biotherapy and Cancer Center/Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, PR China.

To ensure the high purity and biological activity of the adenovirus vector to be used for clinical applications, a stable and linearly scalable preparation method is highly imperative. During the adenovirus-harvesting process, the Triton X-100-based lysis method possesses the advantages of higher efficiency as well as easier linearization and amplification. Most Triton X-100 can be removed from the adenovirus sample by chromatographic purification. However, there is no report that a small amount of residual Triton X-100, present in adenovirus sample, can affect the particle integrity, infectivity, and structure of adenoviruses. Here, we found that although residual Triton X-100 affected the short-term stability, purity, infectivity, and structure of adenoviruses at 37°C, it did not hamper these properties of adenoviruses at 4°C. This study suggests that although the Triton X-100-based lysis method is a simple, efficient, and easy-to-scale process for lysing host cells to release the adenovirus, the storage conditions of adenovirus products must be taken into consideration.
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http://dx.doi.org/10.1016/j.omtm.2020.08.013DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7490641PMC
December 2020

Long non-coding RNA metastasis-associated lung adenocarcinoma transcript 1 regulates renal cancer cell migration via cofilin-1.

Oncol Lett 2020 Oct 27;20(4):53. Epub 2020 Jul 27.

School of Pharmaceutical Science and Technology, Health Science Platform, Tianjin University, Tianjin 300072, P.R. China.

Long non-coding RNA (lncRNA) metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) is upregulated in numerous types of cancer, and is implicated in various cellular processes associated with cancer progression. However, the underlying molecular mechanisms by which MALAT1 regulates metastasis remain unclear. The present study investigated the expression of MALAT1 across a range of different cancer types by analyzing RNA sequencing data from The Cancer Genome Atlas database. The results indicate that the expression of MALAT1 is highly tissue-dependent and that MALAT1 is significantly overexpressed in renal clear cell carcinoma (KIRC). The biological role of MALAT1 in regulating KIRC cell migration was further investigated using molecular and cellular assays. The results demonstrate that MALAT1 regulates the expression of cofilin-1 (CFL1), potentially by regulating RNA splicing. MALAT1 knockdown decreased the expression of CFL1 at both the mRNA and protein levels, and affected cytoskeletal rearrangement by regulating the levels of F-actin via CFL1, leading to significantly decreased cellular migration. Clinical analysis confirmed a significant correlation between MALAT1 and CFL1 expression, implicating both genes as biomarkers for poor prognosis in KIRC. The present study demonstrates a novel mechanism by which MALAT1 regulates cell migration, which may be exploited to develop novel therapeutic strategies for managing renal cancer metastasis.
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http://dx.doi.org/10.3892/ol.2020.11914DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7416383PMC
October 2020

ILC2 Proliferated by IL-33 Stimulation Alleviates Acute Colitis in Rag1 Mouse through Promoting M2 Macrophage Polarization.

J Immunol Res 2020 25;2020:5018975. Epub 2020 Jun 25.

Department of Immunology, China Medical University, No. 77 Puhe Road, Shenyang North New Area, Shenyang, Liaoning Province, China.

This study was to identify functions of ILC2, a newly found innate lymphoid cell which mainly locates in mucosa organs like lungs and intestines, in IBD. We injected rIL-33 protein to C57/BL6 mouse to explore how IL-33 induces ILC2 proliferation. The results showed that ILC2 reached a proliferation peak at day 5 and expressed multiple surface markers like CD127, C-kit, CD69, CD44, ST2, CD27, DR3, MHCII, and CD90.2. ILC2 also expressed high quantity of IL-13 and IL-5 and few IL-17A which indicates a potentially immunological function in IBD development. Afterwards, we transferred sort purified ILC2 to Rag1 mouse given DSS to induce acute colitis in order to explore the innate function of ILC2. Data showed that ILC2 alleviates DSS-induced acute innate colitis by repairing epithelial barrier and restore body weight. Furthermore, we found that ILC2 can cause macrophages polarizing to M2 macrophages in the gut. Therefore, we concluded that ILC2 played a therapeutic role in mouse acute colitis.
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http://dx.doi.org/10.1155/2020/5018975DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7334786PMC
May 2021

Crystal structure of bovine herpesvirus 1 glycoprotein D bound to nectin-1 reveals the basis for its low-affinity binding to the receptor.

Sci Adv 2020 May 13;6(20):eaba5147. Epub 2020 May 13.

West China Hospital Emergency Department (WCHED), State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, and Collaborative Innovation Center of Biotherapy, Chengdu, Sichuan 610041, China.

Bovine herpesvirus 1 (BHV-1) has received increasing attention for its potential oncolytic applications. BHV-1 recognizes nectin-1 for cell entry via viral glycoprotein D (gD) but represents a low-affinity nectin-1 binding virus. The molecular basis underlying this low receptor-binding affinity, however, remains unknown. Here, the crystal structures of BHV-1 gD in the free and nectin-1-bound forms are presented. While showing an overall resembled nectin-1 binding mode to other alphaherpesvirus gDs, BHV-1 gD has a unique G-strand/α2-helix interloop that disturbs gD/nectin-1 interactions. Residue R188 residing in this loop is observed to otherwise cause strong steric hindrance with the bound receptor, making a large conformational change of the loop a prerequisite for nectin-1 engagement. Subsequently, substitution of R188 with glycine markedly enhances the affinity of the BHV-1-gD/nectin-1 interaction (by about fivefold). These structural and functional data delineate the receptor-recognition basis for BHV-1, which might facilitate BHV-1-based oncolytic design in the future.
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http://dx.doi.org/10.1126/sciadv.aba5147DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7220272PMC
May 2020

Solvent triggering structural changes for two terbium-based metal-organic frameworks and their photoluminescence sensing.

Chem Commun (Camb) 2020 Apr 19;56(31):4320-4323. Epub 2020 Mar 19.

School of Materials and Chemical Engineering, Henan International Joint Laboratory of Rare Earth Composite Materials, Henan University of Engineering, Zhengzhou, 451191, P. R. China.

Two terbium-based metal-organic frameworks (TbPDBA-8 and TbPDBA-9) based on the same ligand have been successfully obtained. Only by changing the solvent content, TbPDBA-9 can be transformed into TbPDBA-8, and the addition of sodium formate will accelerate the process. They showed the detection performance of ascorbic acid and HO content respectively.
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http://dx.doi.org/10.1039/d0cc00353kDOI Listing
April 2020

Structural Analysis of Rabies Virus Glycoprotein Reveals pH-Dependent Conformational Changes and Interactions with a Neutralizing Antibody.

Cell Host Microbe 2020 03 30;27(3):441-453.e7. Epub 2020 Jan 30.

West China Hospital Emergency Department (WCHED), State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Collaborative Innovation Center of Biotherapy, Chengdu, Sichuan 610041, China. Electronic address:

Rabies virus (RABV), the etiological agent for the lethal disease of rabies, is a deadly zoonotic pathogen. The RABV glycoprotein (RABV-G) is a key factor mediating virus entry and the major target of neutralizing antibodies. Here, we report the crystal structures of RABV-G solved in the free form at ∼pH-8.0 and in the complex form with a neutralizing antibody 523-11 at ∼pH-6.5, respectively. RABV-G has three domains, and the basic-to-acidic pH change results in large domain re-orientations and concomitant domain-linker re-constructions, switching it from a bent hairpin conformation into an extended conformation. During such low-pH-induced structural transitions, residues located in the domain-linker are found to play important roles in glycoprotein-mediated membrane fusion. Finally, the antibody interacts with RABV-G mainly through its heavy chain and binds to a bipartite conformational epitope in the viral protein for neutralization. These structures provide valuable information for vaccine and drug design.
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http://dx.doi.org/10.1016/j.chom.2019.12.012DOI Listing
March 2020

Nomogram predicting overall survival of rectal squamous cell carcinomas patients based on the SEER database: A population-based STROBE cohort study.

Medicine (Baltimore) 2019 Nov;98(46):e17916

Department of Radiation Oncology, Jilin Cancer Hospital, Changchun, Jilin, China.

We aimed to evaluate the prognostic value of clinical and pathologic factors in rectal squamous cell carcinomas (SCC) and to construct a nomogram for their outcome prediction.The study cohort was selected from Surveillance, Epidemiology, and End Results (SEER) program between January 2004 and December 2013. Univariate and multivariate analyses were performed using Cox proportional hazards regression model to evaluate the prognostic value of involved variables. All prognostic factors were combined to construct a nomogram to predict the overall survival (OS), followed by discrimination as well as calibration plots and receiver operating characteristic (ROC) curves for assessing the predictive accuracy of the nomogram.We identified 806 patients with a median follow-up time of 35 months. Multivariate analyses revealed that marital status (P < .001), age (P < .001), T stage (P = .008), M stage (P < .001), surgery (P = .004), chemotherapy (P = .003) and radiotherapy (P = .016) were independent prognostic factors of OS. Finally, the 7 variables were combined to construct a 3-year and 5-year OS nomogram. The concordance indexes (C-indexes) of OS were 0.756 (95% CI, 0.726-0.786) for the internal validation and 0.729 (95% CI, 0.678-0.780) for the external validation. Additionally, there was superior discrimination power of the nomogram over the SEER stage or the 8th edition AJCC TNM staging classification (P < .001). Calibration plots further showed good consistency between the nomogram prediction and actual observation. The area under the curve (AUC) of ROC curves for 3-year OS was 0.811 (95% CI: 0.769-0.853) in the training cohort and 0.748 (95% CI: 0.681-0.815) in the validation cohort. The AUC for 5-year OS was 0.770 (95% CI: 0.721-0.819) in the training cohort and 0.797 (95% CI: 0.731-0.863) in the validation cohort. Finally, Kaplan-Meier analysis further validates the predictive potential of the nomogram.Marital status, age, T stage, M stage, surgery, chemotherapy and radiotherapy were significantly associated with OS of patients with rectal SCC. This predictive model has the potential to provide an individualized risk estimate of survival in patients with rectal SCC.
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http://dx.doi.org/10.1097/MD.0000000000017916DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6867783PMC
November 2019

The genus Passaloecus Shuckard (Hymenoptera: Crabronidae) from China with four new species and two new records.

Zootaxa 2019 Jul 8;4629(3):zootaxa.4629.3.10. Epub 2019 Jul 8.

Department of Entomology, College of Plant Protection, Yunnan Agricultural University, Kunming, Yunnan, 650201, P. R. China.

Four new species of genus Passaloecus Shuckard, (Crabronidae: Pemphredoninae: Pemphredonini) from China are described, namely P. bisulcatus Bashir Ma, sp. nov., P. profundesulcatus Bashir Ma, sp. nov., P. tuberangustus Bashir Ma, sp. nov., and P. tuberculiformis Bashir Ma, sp. nov. In addition, eight Palearctic species are reported, of which P. turanicus Gussakovskij and P. turionum Dahlbom are first records for China. A key to the species of Passaloecus from China is provided.
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http://dx.doi.org/10.11646/zootaxa.4629.3.10DOI Listing
July 2019

The interaction of YBX1 with G3BP1 promotes renal cell carcinoma cell metastasis via YBX1/G3BP1-SPP1- NF-κB signaling axis.

J Exp Clin Cancer Res 2019 Sep 3;38(1):386. Epub 2019 Sep 3.

The Second Hospital of Tianjin Medical University, Tianjin Institute of Urology and School of Medical Laboratory, Tianjin Medical University, Tianjin, 300070, China.

Background: Renal cell carcinoma (RCC) is a deadly urological tumor that remains largely incurable. Our limited understanding of key molecular mechanisms underlying RCC invasion and metastasis has hampered efforts to identify molecular drivers with therapeutic potential. With evidence from our previous study revealing that nuclear overexpression of YBX1 is associated with RCC T stage and metastasis, we investigated the effects of YBX1 in RCC migration, invasion, and adhesion, and then characterized its interaction with RCC-associated proteins G3BP1 and SPP1.

Methods: Renal cancer cell lines, human embryonic kidney cells, and clinical samples were analyzed to investigate the functional role of YBX1 in RCC metastasis. YBX1 knockdown cells were established via lentiviral infection and subjected to adhesion, transwell migration, and invasion assay. Microarray, immunoprecipitation, dual-luciferase reporter assay, and classical biochemical assays were applied to characterize the mechanism of YBX1 interaction with RCC-associated proteins G3BP1 and SPP1.

Results: Knockdown of YBX1 in RCC cells dramatically inhibited cell adhesion, migration, and invasion. Mechanistic investigations revealed that YBX1 interaction with G3BP1 upregulated their downstream target SPP1 in vitro and in vivo, which led to an activated NF-κB signaling pathway. Meanwhile, knockdown of SPP1 rescued the YBX1/G3BP1-mediated activation of NF-κB signaling pathway, and RCC cell migration and invasion. We further showed that YBX1 expression was positively correlated with G3BP1 and SPP1 expression levels in clinical RCC samples.

Conclusions: YBX1 interacts with G3BP1 to promote metastasis of RCC by activating the YBX1/G3BP1-SPP1-NF-κB signaling axis.
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http://dx.doi.org/10.1186/s13046-019-1347-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6720408PMC
September 2019

Photo-induced electron transfer in a metal-organic framework: a new approach towards a highly sensitive luminescent probe for Fe.

Chem Commun (Camb) 2019 Sep;55(75):11231-11234

State Key Laboratory of Silicon Materials, Cyrus Tang Center for Sensor Materials and Applications, School of Materials Science and Engineering, Zhejiang University, Hangzhou 310027, China.

A Co-based mixed-ligand metal-organic framework (MOF), ZJU-109, is reported by utilizing 6-(4-pyridyl)-terephthalic acid (H2pta) and 4,4'-bis(imidazolyl)biphenyl (4,4'-bimbp) as double linkers. By tuning the d-PET process between ligands 4,4'-bimbp and H2pta, a fluorescent turn-on sensor for Fe3+ with a detection limit as low as 0.053 μM has been demonstrated.
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http://dx.doi.org/10.1039/c9cc05019aDOI Listing
September 2019

Development of a new monoclonal antibody specific to mouse Vγ6 chain.

Life Sci Alliance 2019 06 7;2(3). Epub 2019 May 7.

Division of Host Defense, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan.

There are seven Vγ gene segments in the TCR γ chain loci of mice. We developed monoclonal antibodies (mAbs) specific to the Vγ6 chain (Heilig & Tonegawa nomenclature). By immunizing Vγ4/6 KO mice with complementarity-determining region peptides in Vγ6 chains, we generated three hybridomas. These hybridomas produced mAbs capable of cell surface staining of Vγ6/Vδ1 gene-transfected T-cell line lacking TCR as well as of Vγ1 Vγ4 Vγ5 Vγ7 γδ T cells and the CD3 TCRδ γδ T cells in various organs. The location of Vγ6 γδ T cells, which peaked in the newborn thymus, was associated with mTEC. In vivo administration of clone 1C10-1F7 mAb impaired protection against infection but ameliorated psoriasis-like dermatitis induced by imiquimod treatment. These new mAbs are useful to elucidate the development, location, and functions of Vγ6 γδ T cells in mice.
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http://dx.doi.org/10.26508/lsa.201900363DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6504751PMC
June 2019

CD30L/CD30 protects against psoriasiform skin inflammation by suppressing Th17-related cytokine production by Vγ4 γδ T cells.

J Autoimmun 2019 07 18;101:70-85. Epub 2019 Apr 18.

Department of Immunology, China Medical University, No.77 Puhe Road, Shenyang North New Area, Shenyang, Liaoning Province, PR China. Electronic address:

Psoriasis is a common, autoimmune, chronic inflammatory skin disease. It has been demonstrated that cutaneous T17 cells play an important pro-inflammatory role in the pathogenesis of psoriasis, through the production of various Th17-related cytokines. Our previous studies have demonstrated that CD30L/CD30 signal plays a pivotal role in the differentiation of CD4 Th17 cells and Vγ6γδ T17 cells in the gut-associated lymphoid tissues of mouse. However, its effect on the pathogenesis of psoriasis is unknown. Here, we fully prove that CD30L/CD30 signaling plays a novel protective role in the development of psoriasis in mice, through selective inhibition of CCR6 expression and Th17-related cytokine synthesis in the Vγ4γδ T17 cell subset. Meanwhile, treatment with agonistic anti-CD30 mAb had a significant therapeutic effect on our psoriasis mouse model. Therefore, the CD30L/CD30 signaling pathway is an ideal target for antibody therapy, which may become a new approach for the immunobiological treatment of psoriasis.
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http://dx.doi.org/10.1016/j.jaut.2019.04.009DOI Listing
July 2019

CUL4B promotes aggressive phenotypes of HNSCC via the activation of the Wnt/β-catenin signaling pathway.

Cancer Med 2019 05 18;8(5):2278-2287. Epub 2019 Mar 18.

Department of Laboratory Medicine, ShengJing Hospital of China Medical University, Shenyang, China.

Previous studies have revealed that CUL4B is overexpressed in various types of cancer and that its overexpression is related to the progression and metastasis of tumors. However, the biological functions of CUL4B in the progression of head and neck squamous cell carcinoma (HNSCC) are still not well understood. In the current study, we aimed to determine the changes in biological functions and molecular events that are related to CUL4B overexpression. Interestingly, our results showed that CUL4B is upregulated in HNSCC and that its upregulation is associated with poor survival and worse histological grade. Overexpression of CUL4B promoted cancer cell growth, invasion, and migration, as well as epithelial-mesenchymal transition, whereas the loss of CUL4B abrogated these malignant phenotypes. Moreover, our mechanistic investigations suggest that the Wnt/β-catenin signaling pathway was activated by CUL4B overexpression. Treatment with a Wnt/β-catenin inhibitor decreased CUL4B-induced migration and invasion, establishing a key role of Wnt/β-catenin signaling in mediating the effects of CUL4B expression. Together, these results demonstrate a key contribution of CUL4B overexpression in the malignant behavior of HNSCC cells, at least in part through the stimulation of angiogenesis and the activation of the Wnt/β-catenin signaling pathway.
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http://dx.doi.org/10.1002/cam4.1960DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6536945PMC
May 2019

Isostructural Tb/Eu Co-Doped Metal-Organic Framework Based on Pyridine-Containing Dicarboxylate Ligands for Ratiometric Luminescence Temperature Sensing.

Inorg Chem 2019 Feb 31;58(4):2637-2644. Epub 2019 Jan 31.

State Key Laboratory of Silicon Materials, Cyrus Tang Center for Sensor Materials and Applications, School of Materials Science and Engineering , Zhejiang University , Hangzhou 310027 , China.

In this work, we prepared two types of isostructural Ln-based metal-organic frameworks (LnMOFs) under solvothermal conditions, where two structurally similar pyridine-containing dicarboxylate ligands, 6-(4-carboxyphenyl)nicotinic acid and [2,2'-bipyridine]-5,5'-dicarboxylic acid, were used as the organic linkers. The as-synthesized LnMOF compounds were characterized using single-crystal X-ray diffraction (XRD), powder XRD, and thermogravimetric analysis. With the lanthanide co-doping approach, two mixed LnMOFs, TbEucpna and TbEubpydc, were obtained and evaluated for application as potential ratiometric luminescence thermometers. The temperature-dependent luminescence of the two materials was investigated, and their emission intensities, luminescence lifetimes, and thermometric parameters were compared. They exhibit an excellent S-shaped response for temperatures in the range of 25-300 K, with favorable relative sensitivity and temperature uncertainty. Moreover, their color changes from green at 25 K to red at 300 K, so that they are also suitable as colorimetric luminescent probes.
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http://dx.doi.org/10.1021/acs.inorgchem.8b03225DOI Listing
February 2019

Ixazomib promotes CHOP-dependent DR5 induction and apoptosis in colorectal cancer cells.

Authors:
Dan Yue Xun Sun

Cancer Biol Ther 2019 25;20(3):284-294. Epub 2018 Oct 25.

b Department of Immunology , China Medical University , Shenyang , China.

Background: Ixazomib (Ninlaro), a novel proteasome inhibitor, has been developed for the treatment of many cancers and has demonstrated anti-tumor efficacy against various malignancies. However, the mechanism of the anti-tumor effect of ixazomib in colorectal cancer (CRC) cells remains unclear.

Methods: MTS and flow cytometry were performed to determine the effect of ixazomib on CRC cells. Western blotting and real-time RT-PCR were performed to detect ixazomib-induced DR5 upregulation. ChIP was performed to detect CHOP binding to DR5 promoter. Finally, xenograft experiments were carried out to measure the antitumor effect of ixazomib in vivo.

Results: In this study, we revealed the mechanism by which ixazomib inhibits the growth of CRC cells. Our findings indicated that ixazomib treatment induces CHOP-dependent DR5 induction, irrespective of p53 status. Furthermore, DR5 is necessary for ixazomib-mediated apoptosis. Ixazomib also synergized with TRAIL to induce marked apoptosis via DR5 in CRC cells.

Conclusions: Our findings further suggested that ixazomib sensitizes TRAIL/death receptor signaling pathway-targeted CRC and suggested that DR5 induction could be a valuable indicator of ixazomib sensitivity.
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http://dx.doi.org/10.1080/15384047.2018.1529095DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6370389PMC
May 2020

The Role of YB1 in Renal Cell Carcinoma Cell Adhesion.

Int J Med Sci 2018 6;15(12):1304-1311. Epub 2018 Aug 6.

Department of Urology, The Second Hospital of Tianjin Medical University, Tianjin Institute of Urology and Department of Microbiology, School of Medical Laboratory, Tianjin Medical University, Tianjin 300070, China.

Y-box binding protein 1 (YB1) is a multifunctional protein involved in many processes related to cancer progression and metastasis. In this study, we constructed YB1 knockdown stable renal cell carcinoma (RCC) cell line 786-0. The gene expression profile of 786-0 was performed by DNA microarray analysis to identify genes that were regulated by YB1. Real-time PCR and western blotting were used to test the genes and proteins expression. Transforming growth factor-β (TGF-β) activity was detected by dual-luciferase reporter assay. Cell adhesion assay was used to determine RCC cell adhesion ability. Pathway analysis revealed that YB1 knockdown influenced cell adhesion molecules (CAMs). We further verified four genes ( and ) related to CAMs by real-time PCR, and confirmed that YB1 regulated the expression of ITGB8 in RCC. Functional assays demonstrated that knockdown of YB1 significantly inhibited the cell adhesion of 786-0 cells . In addition, YB1 affected TGF-β activation. Our study demonstrated that YB1 modulated the adhesion ability of renal cell carcinoma cells by regulating ITGB8 and TGF-β.
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http://dx.doi.org/10.7150/ijms.25580DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6158664PMC
January 2019

Cryogenic Luminescent Tb/Eu-MOF Thermometer Based on a Fluorine-Modified Tetracarboxylate Ligand.

Inorg Chem 2018 Oct 26;57(20):12596-12602. Epub 2018 Sep 26.

State Key Laboratory of Silicon Materials, Cyrus Tang Center for Sensor Materials and Applications, School of Materials Science and Engineering , Zhejiang University , Hangzhou 310027 , China.

A fluorine-modified tetracarboxylic acid ligand, namely, 2'-fluoro-[1,1':4',1''-terphenyl]-3,3'',5,5''-tetracarboxylic acid (HFTPTC), with suitable triplet energy excited state, was designed and applied to construct the luminescent lanthanide metal-organic frameworks (LnMOFs) for cryogenic temperature sensing. With the lanthanides codoping strategy, we developed a new Tb/Eu mixed LnMOF system TbEu FTPTC ( x = 0.05, 0.1, 0.2), which feature excellent linear responses to temperature with high relative sensitivity in the cryogenic range of 25-125 K. It was found that the relative sensitivity of such mixed LnMOF could readily be tuned by adjusting the incorporation amount of Eu ions in the host framework. In addition, the energy transfer efficiency between the Tb and Eu ions in the framework with different Tb/Eu ratios are also investigated and discussed.
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http://dx.doi.org/10.1021/acs.inorgchem.8b01746DOI Listing
October 2018

Idelalisib promotes Bim-dependent apoptosis through AKT/FoxO3a in hepatocellular carcinoma.

Authors:
Dan Yue Xun Sun

Cell Death Dis 2018 09 17;9(10):935. Epub 2018 Sep 17.

Department of Immunology, China Medical University, Shenyang, China.

Idelalisib, a selective PI3Kδ inhibitor, has been approved by the FDA for chronic lymphocytic leukemia/small lymphocytic lymphoma treatment and for follicular lymphoma treatment when combined with rituximab. However, the mechanisms of effective action of idelalisib in hepatocellular carcinoma (HCC) remain unclear. In the current study, we aimed to investigate how idelalisib inhibits the growth of HCC cells and enhances the effects of other chemotherapeutic drugs. Our results show that idelalisib treatment promotes Bim induction in HCC via the FoxO3a pathway following PI3K/AKT inactivation. Moreover, our results show that Bim is required for idelalisib-mediated apoptosis in HCC. Idelalisib also synergizes with sorafenib or doxorubicin to induce significant apoptosis in HCC, and Bim is also necessary for the induction of apoptosis by cotreatment. Furthermore, a xenograft experiment reveals that the Bim deficiency abolishes apoptosis and antitumor effects of idelalisib in vivo. In summary, our results indicate a key role of Bim in mediating the antitumor effects of idelalisib in HCC. Our results also support the clinical significance of the drug.
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http://dx.doi.org/10.1038/s41419-018-0960-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6141589PMC
September 2018

Quantitative Proteomics Implicates Rictor/mTORC2 in Cell Adhesion.

J Proteome Res 2018 10 10;17(10):3360-3369. Epub 2018 Sep 10.

Department of Genetics, School of Basic Medical Sciences , Tianjin Medical University , Tianjin 300070 , P.R. China.

The mammalian target of rapamycin complex 2 (mTORC2) plays critical roles in various biological processes. To better understand the functions of mTORC2 and the underlying molecular mechanisms, we established a stable cell line with reduced Rictor, a specific component in mTORC2, and investigated the quantitative changes of the cellular proteome. As a result, we observed that 101 proteins were down-regulated and 50 proteins were up-regulated in Rictor knockdown cells. A protein-protein interaction network regulated by Rictor/mTORC2 was established, showing that Rictor/mTORC2 was involved in various cellular processes. Intriguingly, gene ontology analysis indicated that the proteome regulated by Rictor/mTORC2 was significantly involved with cell adhesion. Rictor knockdown affected the expressions of multiple cell adhesion associated molecules, e.g. integrin α-5 (ITGA5), transforming growth factor beta-1-induced transcript 1 protein (TGFB1I1), lysyl oxidase homologue 2 (LOXL2), etc. Further study suggested that Rictor/mTORC2 may regulate cell adhesion and invasion by modulating the expressions of these cell adhesion molecules through AKT. Taken together, this study maps the proteome regulated by Rictor/mTORC2 and reveals its role in promoting renal cancer cell invasion through modulating cell adhesion and migration.
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http://dx.doi.org/10.1021/acs.jproteome.8b00218DOI Listing
October 2018

A luminescent turn-up metal-organic framework sensor for tryptophan based on singlet-singlet Förster energy transfer.

J Mater Chem B 2018 Aug 31;6(31):5174-5180. Epub 2018 Jul 31.

State Key Laboratory of Silicon Materials, Cyrus Tang Center for Sensor Materials and Applications, School of Materials Science and Engineering, Zhejiang University, Hangzhou 310027, China.

A water stable metal-organic framework, ZJU-108, was designed and successfully synthesized as a luminescent turn-up sensor, and it can discriminate tryptophan from other natural amino acids in aqueous solution. Because of the apparent lower singlet level of tryptophan compared with other amino acids, the singlet-singlet Förster energy transfer mechanism was applied to design a luminescent sensor. As a result, a selective MOF sensor for tryptophan was realized by choosing the ligand with the appropriate singlet energy level. As expected, ZJU-108 shows an excellent luminescence enhancement response to tryptophan over other natural amino acids with a detection limit of 42.9 nM. This work presents a new insight for designing turn-up MOF sensors and demonstrates the potential of ZJU-108 as a luminescent probe for the detection of tryptophan.
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http://dx.doi.org/10.1039/c8tb01592aDOI Listing
August 2018

Co-expression of ApGSMT2g and ApDMT2g in cotton enhances salt tolerance and increases seed cotton yield in saline fields.

Plant Sci 2018 Sep 15;274:369-382. Epub 2018 Jun 15.

The Key Laboratory of the Plant Cell Engineering and Germplasm Innovation, School of Life Science, Shandong University, Qingdao 266237, Shandong Province, China. Electronic address:

Salinity is a major factor limiting plant growth and agricultural production worldwide. Glycine betaine (GB) is one of the most universal osmoprotectants that protects plants from environmental stresses. In this study, transgenic cotton co-expressing ApGSMT2g and ApDMT2g was generated by Agrobacterium-mediated transformation. Compared with wild-type (WT), co-expression of ApGSMT2g and ApDMT2g in cotton results in higher GB amounts, higher relative water content (RWC), lower osmotic potential, more K, and less Na under salt stress, which contributes to maintaining intracellular osmoregulation and K/Na homeostasis and thus confers higher salt tolerance and more vigorous growth. Furthermore, co-expressing ApGSMT2g and ApDMT2g in cotton leads to better performance of PSII, greater photosynthesis capacity, and finally, improves plant growth and increases cotton seed yield compared to WT under salt stress. The reason for the better performance of PSII in transgenic cotton is the higher quantum yield and a more reasonable quantum ratio distribution than WT under salt stress. Co-expressing ApGSMT2g and ApDMT2g in cotton also reduces membrane damage and increases superoxide dismutase (SOD) activity compared to WT under salt stress. Our results indicate that transgenic ApGSMT2g and ApDMT2g cotton shows higher salt tolerance and more seed cotton yield in saline fields compared to wild-type.
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http://dx.doi.org/10.1016/j.plantsci.2018.06.007DOI Listing
September 2018

Flexible Metal-Organic Framework-Based Mixed-Matrix Membranes: A New Platform for H S Sensors.

Small 2018 Sep 26;14(37):e1801563. Epub 2018 Jul 26.

State Key Laboratory of Silicon Materials, Cyrus Tang Center for Sensor Materials and Applications, School of Materials Science and Engineering, Zhejiang University, Hangzhou, 310027, China.

Metal-organic framework (MOF)-polymer mixed-matrix membranes (MMMs) have shown great potential and superior performance in gas separations. However, their sensing application has not been fully established yet. Herein, a rare example of using flexible MOF-based MMMs as a fluorescent turn-on sensor for the detection of hydrogen sulfide (H S) is reported. These MOF-based MMMs are readily prepared by mixing a highly stable aluminum-based nano-MOF (Al-MIL-53-NO ) into poly(vinylidene fluoride) with high loadings up to 70%. Unlike the intrinsic fragility and poor processability of pure-MOF membranes, these MMMs exhibit desirable flexibility and processability that are more suitable for practical sensing applications. The uniform distribution of Al-MIL-53-NO particles combined with the permanent pores of MOFs enable these MMMs to show good water permeation flux and consequently have a full contact between the analyte and MOFs. The developed MMM sensor (70% MOF loading) thus shows a highly remarkable detection selectivity and sensitivity for H S with an exceptionally low detection limit around 92.31 × 10 m, three orders of magnitude lower than the reported powder-form MOFs. This work demonstrates that it is feasible to develop flexible luminescent MOF-based MMMs as a novel platform for chemical sensing applications.
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http://dx.doi.org/10.1002/smll.201801563DOI Listing
September 2018

miR-182 regulates trastuzumab resistance by targeting MET in breast cancer cells.

Cancer Gene Ther 2019 02 21;26(1-2):1-10. Epub 2018 Jun 21.

Laboratory Medicine Department, Sheng Jing Hospital of China Medical University, 36 Sanhao St, Heping Qu, 110003, Shenyang, China.

It has been found that microRNAs (miRNAs) play a key role in drug resistance. The purpose of the current study was to investigate the function of miR-182 in trastuzumab resistance in breast cancer cells. The results showed that both breast cancer SKBR3 trastuzumab-resistant cells (SKBR3/TR) and BT474 trastuzumab-resistant cells (BT474/TR) were associated with miR-182 downregulation compared with their parental cells. Ectopic expression of the miR-182 mimic inhibited trastuzumab resistance, decreasing the invasion and migration of these trastuzumab-resistant cells. However, the miR-182 inhibitor increased trastuzumab resistance, cell invasion, and migration in the parental cells. In addition, MET is a directly targeted gene of miR-182 in breast cancer cells. MET knockdown showed an inhibitory effect of trastuzumab resistance on trastuzumab-resistant cells. In contrast, MET overexpression in SKBR3 cells produced an effect that promotes resistance to trastuzumab. Moreover, we revealed that overexpression of miR-182 reduced trastuzumab resistance in trastuzumab-resistant cells due in part to MET/PI3K/AKT/mTOR signaling pathway inactivation. Furthermore, miR-182 could also sensitize SKBR3/TR cells to trastuzumab in vivo. In conclusion, our results suggest that the activation of miR-182 or inactivation of its target gene pathway could be used as a new method to reverse trastuzumab resistance in breast cancer.
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http://dx.doi.org/10.1038/s41417-018-0031-4DOI Listing
February 2019
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