Publications by authors named "Dan Tian"

150 Publications

Double-Walled Metal-Organic Framework with Regulable Pore Environments for Efficient Removal of Radioactive Cesium Cations.

Inorg Chem 2021 Aug 4. Epub 2021 Aug 4.

School of Materials Science and Engineering, Tianjin Key Laboratory of Metal and Molecule-Based Material Chemistry, Nankai University, Tianjin 300350, China.

An anion double-walled metal-organic framework [CoLi(BTC)(DMF)(HO)·(CH)N] () based on heterobimetallic Li and Co ions was successfully constructed. Utilizing selective destruction and formation of Co-O/Co-N bonds in the metal chains, [CoLi(BTC)(py)(HO)·(CH)N] () and [CoLi(BTC)(pi)(HO)·(CH)N] () with the same skeleton but distinct pore structures can be surprisingly obtained. Additionally, compounds and can be transformed into [CoLi(BTC)(HO)·(CH)N] () by soaking them in an ethanol solution. This kind of single-crystal-to-single-crystal transformation successfully regulates the pore structure of MOFs and enriches the diversity of the pore wall on the premise of retaining the original framework. Most impressively, compound shows high adsorption capacity for Cs cations and is a good candidate to selectively accommodate Cs among the common alkali metal ions, which is future identified by single-crystal X-ray diffraction and inductively coupled plasma mass spectrometry (ICP-MS) test. Meanwhile, compound can selectively adsorb methylene blue (MB) and crystal violet (CV) molecules over Rhodamine B (RMB).
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http://dx.doi.org/10.1021/acs.inorgchem.1c01260DOI Listing
August 2021

Anti-inflammatory effects of microRNA-223 on sepsis-induced lung injury in rats by targeting the Toll-like receptor signaling pathway.

Exp Ther Med 2021 Sep 7;22(3):964. Epub 2021 Jul 7.

Department of Clinical Laboratory, The Fourth Central Hospital of Baoding City, Baoding, Hebei 072350, P.R. China.

The aim of the present study was to investigate the mediation of micro RNA (miR)-223 on the anti-inflammatory effect of the Toll-like receptor (TLR) signaling pathway on sepsis-induced lung injury in rats via negatively regulating the expression of interleukin (IL)-6. Sprague-Dawley rats were used in the present study. It was determined whether miR-223 is differentially expressed in the lung using reverse transcription-quantitative PCR techniques and the content of cytokines in bronchoalveolar lavage (BAL) fluid was detected. The protein expression levels of TLR4 and nuclear factor (NF)-κB p65 were examined by western blotting and the pathological changes in the lung tissues of the sepsis group were observed. Hematoxylin and eosin was used to stain the lung tissues. The alveoli in the sham group exhibited a normal structure and morphology. In the sepsis group, the alveoli of the lung tissues were surrounded by numerous neutrophils, the mesenchyme was swollen, regions of the alveolar wall exhibited fibrosis and the alveolar wall was thickened. Furthermore, in the sepsis group, miR-223 expression was increased in the lung tissues when compared with that in the sham group. The content of cytokines, IL-6 and IL-1β in the BAL fluid was significantly increased when compared with that of the sham group and TLR4 and NF-κB were also highly expressed. In addition, when compared with RAW264.7 cells that were overexpressing miR-223, the content of IL-6 and IL-1β in the supernatant and protein expression of TLR and NF-κB in cells were markedly decreased. Thus, it was demonstrated that miR-223 negatively regulated the expression of IL-6, mediating the TLR4/NF-κB signaling pathway and exerting an anti-inflammatory effect in sepsis-induced lung injury.
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http://dx.doi.org/10.3892/etm.2021.10396DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8290467PMC
September 2021

Surgical resection of primary tumors improved the prognosis of patients with bone metastasis of non-small cell lung cancer: a population-based and propensity score-matched study.

Ann Transl Med 2021 May;9(9):775

Department of Thoracic Surgery, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China.

Background: Most patients with lung cancer are in an advanced stage at the time of diagnosis due to occult onset. Bone is one of the most common sites of hematogenous metastasis of lung cancer. This study aimed to evaluate the impact of surgical resection of primary tumors on the prognosis of patients with bone metastasis of non-small cell lung cancer, using the Surveillance, Epidemiology, and End Results (SEER) database.

Methods: A total of 9,804 patients with only bone metastasis were identified from the SEER database. Propensity score matching was used to reduce the selection bias. Cancer-specific survival (CSS) and overall survival (OS) were compared between patients with or without primary tumor resection. The Cox regression model was applied to evaluate multiple prognostic factors.

Results: After propensity score matching, 424 patients were selected for survival analysis. No statistically significant differences were found in age, sex, race, tumor location, histology, T stage, and N stage between patients with or without surgical resection of primary tumors. The prognosis of patients who underwent surgical resection of primary tumors was significantly better than that of patients who had not undergone surgery. The surgical resection of primary tumors was an independent prognostic factor. The prognosis of patients who underwent lobectomy/bilobectomy was significantly better compared to other surgical types. Regional lymph node resection during surgery also significantly improved the prognosis of the patients.

Conclusions: For patients with only bone metastasis, surgical resection of primary tumors could significantly improve prognosis. Lobectomy/bilobectomy with regional lymph node resection was the best surgical strategy.
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http://dx.doi.org/10.21037/atm-21-540DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8246174PMC
May 2021

Inhibition of Perforin-Mediated Neurotoxicity Attenuates Neurological Deficits After Ischemic Stroke.

Front Cell Neurosci 2021 28;15:664312. Epub 2021 Jun 28.

Department of Neurology, Beijing Friendship Hospital, Capital Medical University, Beijing, China.

Perforin-mediated cytotoxicity plays a crucial role in microbial defense, tumor surveillance, and primary autoimmune disorders. However, the contribution of the cytolytic protein perforin to ischemia-induced secondary tissue damage in the brain has not been fully investigated. Here, we examined the kinetics and subpopulations of perforin-positive cells and then evaluated the direct effects of perforin-mediated cytotoxicity on outcomes after ischemic stroke. Using flow cytometry, we showed that perforinCD45 immune cells could be detected at 12 h and that the percentage of these cells increased largely until on day 3 and then significantly declined on day 7. Surprisingly, the percentage of PerforinCD45 cells also unexpectedly increased from day 7 to day 14 after ischemic stroke in Perforin1-EGFP transgenic mice. Our results suggested that PerforinCD45 cells play vital roles in the ischemic brain at early and late stages and further suggested that PerforinCD45 cells are a heterogeneous population. Surprisingly, in addition to CD8 T cells, NK cells, and NKT cells, central nervous system (CNS)-resident immune microglia, which are first triggered and activated within minutes after ischemic stroke in mice, also secreted perforin during ischemic brain injury. In our study, the percentage of perforin microglia increased from 12 h after ischemic stroke, increased largely until on day 3 after ischemic stroke, and then moderately declined from days 3 to 7. Intriguingly, the percentage of perforin microglia also dramatically increased from days 7 to 14 after ischemic stroke. Furthermore, compared with wild-type littermates, Perforin 1 mice exhibited significant increases in the cerebral infarct volume, neurological deficits, and neurogenesis and inhibition of neurotoxic astrogliosis. Interestingly, the number of CD45CD3 T cells was significantly decreased in Perforin 1 mice compared with their wild-type littermates, especially the number of γδ T cells. In addition, Perforin 1 mice had lower levels of IL-17 than their wild-type littermates. Our results identified a critical function of perforin-mediated neurotoxicity in the ischemic brain, suggesting that targeting perforin-mediated neurotoxicity in brain-resident microglia and invading perforinCD45 immune cells may be a potential strategy for the treatment of ischemic stroke.
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http://dx.doi.org/10.3389/fncel.2021.664312DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8274971PMC
June 2021

Novel Mutations and Clinical Characteristics in Seven Chinese Families With Congenital Nephrogenic Diabetes Insipidus.

Front Endocrinol (Lausanne) 2021 10;12:686818. Epub 2021 Jun 10.

Department of Endocrinology, Key Laboratory of Endocrinology, NHC, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing, China.

Objective: Mutations in lead to rare congenital nephrogenic diabetes insipidus (NDI), which has been limitedly studied in Chinese population.

Methods: Twenty-five subjects from seven families with NDI in a department (Beijing, PUMCH) were screened for mutations. Clinical characteristics were described and genotype-phenotype correlation analysis was performed.

Results: We identified 9 mutations in 13 patients with NDI, including 3 novel mutations (p.G165D, p.Q255RfsTer72 and IVS3-3delC). Missense mutations were the most common mutation type, followed by splicing mutations, and frameshift mutations caused by small deletion or insertion. The onset-age in our patients was younger than 1 year old. Common manifestations included polydipsia, polyuria (7/7) and intermittent fever (6/7). Less common presentations included short stature (3/7) and mental impairment (1/7). High osmotic hypernatremia and low osmotic urine were the main biochemical features. Dilation of the urinary tract was a common complication of NDI (3/6). Level of serum sodium in NDI patients with compound het AQP2 mutations was higher than non-compound het mutations.

Conclusion: In the first and largest case series of NDI caused by mutation in Chinese population, we identified 9 mutations, including 3 novel mutations. Phenotype was found to correlate with genotypes, revealed by higher level of serum sodium in patients with compound het mutations than non-compound het mutations. This knowledge broadens genotypic and phenotypic spectrum for rare congenital NDI and provided basis for studying molecular biology of AQP2.
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http://dx.doi.org/10.3389/fendo.2021.686818DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8225504PMC
June 2021

Fluorine-induced aggregate-interlocking for color-tunable organic afterglow with a simultaneously improved efficiency and lifetime.

Chem Sci 2021 Jan 12;12(10):3580-3586. Epub 2021 Jan 12.

Key Laboratory for Organic Electronics and Information Displays & Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials (IAM), Nanjing University of Posts & Telecommunications 9 Wenyuan Road Nanjing 210023 China

Designing organic afterglow materials with a high efficiency and long lifetime is highly attractive but challenging because of the inherent competition between the luminescence efficiency and lifetime. Here, we propose a simple yet efficient strategy, namely fluorine-induced aggregate-interlocking (FIAI), to realize both an enhanced efficiency and elongated lifetime of afterglow materials by stimulating the synergistic effects of the introduced fluorine atoms to efficiently promote intersystem crossing (ISC) and intermolecular non-covalent interactions for facilitating both the generation of triplet excitons and suppression of non-radiative decays. Thus, the fluorine-incorporated afterglow molecules exhibit greatly enhanced ISC with a rate constant up to 5.84 × 10 s and suppressed non-radiative decay down to 0.89 s, resulting in efficient organic afterglow with a simultaneously improved efficiency up to 10.5% and a lifetime of 1.09 s. Moreover, accompanied by the efficient phosphorescence emission especially at cryogenic temperature, color-tunable afterglow was also observed at different temperatures. Therefore, tri-mode multiplexing encryption devices by combining lifetime, temperature and color, and visual temperature sensing were successfully established. The FIAI strategy by addressing fundamental issues of afterglow emission paves the way to develop high-performance organic afterglow materials, opening up a broad prospect of aggregated and excited state tuning of organic solids for emission lifetime-resolved applications.
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http://dx.doi.org/10.1039/d0sc06025aDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8179499PMC
January 2021

Dietary, physical exercises and mental stress in a Chinese population: a cross-sectional study.

BMC Public Health 2021 06 14;21(1):1138. Epub 2021 Jun 14.

Department of Health Management, School of Public Health, Center for Global Health, Nanjing Medical University, Nanjing, 211166, China.

Background: Mental health is affected by both genetic and environmental factors. However, previous studies have showed conflict findings about the role of lifestyle and little is known about the situation of the Chinese population. The purpose of this study was to explore the relationship between the frequency of food consumption, physical exercise condition and mental health, as well as factors related to mental stress in Chinese.

Methods: We recruited 8160 residents who had health examinations in a public hospital during June 2016 to May 2018. Demographic characteristics, the frequency of food consumption, physical exercise condition and mental health status was collected by a questionnaire. We estimated the association using the odds ratio (OR) and 95% confidence interval (CI) based on Binary or ordinal logistic regression models. A classification and regression tree (CART) demonstrated the prediction of the value of a target variable based on other values.

Results: The logistic regression model and classification tree model both found that the frequency of fresh vegetables or fruit and fried foods consumption and the current state of drinking alcohol were related to mental stress. The degree of mental stress reduced significantly with increasing consumption of fish (OR = 0.80, 95% CI: 0.71-0.90) and regular exercise (OR = 0.55, 95% CI:0.48-0.64) in females and increased consumption of fish (OR = 0.55, 95% CI: 0.48-0.64) and cereal crop (OR = 0.77, 95% CI: 0.68-0.89), fish (OR = 0.87, 95%CI:0.77-0.96) and regular exercise (OR = 0.61, 95%CI:0.53-0.70) in males. On the contrary, the frequency of consumption of desserts (OR = 1.43, 95% CI: 1.26-1.62) and the current of drinking alcohol (OR = 1.47, 95%CI:1.21-1.79) in females and meat (OR = 1.47, 95%CI: 1.31-1.65), pickled and smoked food (OR = 1.18, 95%CI:1.05-1.32) and the current state of drinking alcohol (OR = 1.25, 95%CI:1.12-1.40) in males were related to an increased risk of mental health.

Conclusions: Our study showed that both the frequency of some food consumption and physical exercise condition were associated with mental health and affected the degree of stress, which provided novel insights into interventions.
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http://dx.doi.org/10.1186/s12889-021-11189-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8201724PMC
June 2021

Surgery or Non-surgical Treatment of ≤8 mm Non-small Cell Lung Cancer: A Population-Based Study.

Front Surg 2021 26;8:632561. Epub 2021 May 26.

Department of Thoracic Surgery, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China.

Timing for intervention of small indeterminate pulmonary nodules has long been a topic of debate given the low incidence of malignancy and difficulty in obtaining a definite preoperative diagnosis. We sought to determine survival outcomes of surgical and non-surgical managements in non-small cell lung cancer (NSCLC) ≤8 mm, which may provide a reference for prospective decision-making for patients with suspected NSCLC. A total of 1,652 patients with Stage IA NSCLC ≤8 mm were identified from the Surveillance, Epidemiology, and End Results (SEER) database and categorized into surgery and non-surgery groups. Chi-square test, -test and Mann-Whitney U test were used to compare the baseline characteristics between groups. Survival curves were depicted using Kaplan-Meier method and compared by log-rank test. Cox proportional hazard model was used for univariate and multivariate analyses. Adjustment of confounding factors between groups was performed by propensity score matching. The surgery and non-surgery groups included 1,438 and 208 patients, respectively. Patients in surgery group demonstrated superior survival outcome than patients in non-surgery group both before [overall survival (OS): HR, 16.22; 95% CI, 11.48-22.91, < 0.001; cancer-specific survival (CSS): HR, 49.6; 95% CI, 31.09-79.11, < 0.001] and after (OS: HR, 3.12; 95% CI, 2.40-4.05, < 0.001; CSS: HR, 3.85; 95% CI, 2.74-5.40, < 0.001) propensity score matching. The 30-day mortality rates were 3.1 and 12.0% in surgery and non-surgery groups, respectively. Multivariate analysis suggested age, sex, race, tumor size, grade, pathological stage were all independent prognostic factors in patients with ≤8 mm NSCLC. A comparison of surgical resections revealed a survival superiority of lobectomy over sub-lobectomy. In terms of CSS, no statistically significant difference was found between segmentectomy and wedge resection. The current SEER database showed better prognosis of surgical resection than non-surgical treatment in patients with ≤8 mm NSCLC. However, the factors that should be essentially included in the proper propensity-matched analysis, such as comorbidity, cardiopulmonary function and performance status were unavailable and the true superiority or inferiority should be examined further by ongoing randomized trial, especially comparing surgery and stereotactic body irradiation.
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http://dx.doi.org/10.3389/fsurg.2021.632561DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8187794PMC
May 2021

The VDR gene confers a genetic predisposition to Graves' disease and Graves' ophthalmopathy in the Southwest Chinese Han population.

Gene 2021 Aug 30;793:145750. Epub 2021 May 30.

School of Basic Medical Sciences, Special Key Laboratory of Ocular Diseases of Guizhou Province, Zunyi Medical University, Guizhou 563000, China. Electronic address:

Objective: Graves' disease (GD) is a common autoimmune disease manifesting with diffuse symmetric thyroid gland enlargement, pretibial myxedema, and Graves' ophthalmopathy (GO). Recently, the vitamin D receptor (VDR) gene has been linked to various autoimmune diseases. This study aimed to investigate the association of VDR gene polymorphisms with susceptibility to GD and GO in the Southwest Chinese Han population.

Methods: A two-stage association study was performed in 1,209 controls and 650 GD patients by PCR-RFLP assay. Real-time PCR and ELISA were carried out to quantify gene expression and cytokine production.

Results: The first-stage study showed that the frequency of VDR/Apa I AA genotype was significantly increased in GD (Pc = 1.67 × 10, OR = 1.98). The second-stage and combined studies confirmed the association of VDR/Apa I with GD (AA genotype: Pc = 3.45 × 10, OR = 1.87; A allele: Pc = 2.62 × 10, OR = 1.20). The stratification analysis showed that GO patients had a higher frequency of the VDR/Apa I AA genotype (Pc = 8.69 × 10, OR = 2.84). Functional experiments showed a decreased VDR expression and TGF-β1 production as well as an increased IL-17 production in VDR/Apa I AA genotype carriers.

Conclusion: The VDR/Apa I polymorphism is significantly associated with GD and GO, and it may be involved in the development of GD and GO by influencing VDR mRNA expression levels and the secretion levels of cytokines.
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http://dx.doi.org/10.1016/j.gene.2021.145750DOI Listing
August 2021

Transcriptome landscape of double negative T cells by single-cell RNA sequencing.

J Autoimmun 2021 Jul 19;121:102653. Epub 2021 May 19.

General Surgery Department, Beijing Friendship Hospital, Capital Medical University, Beijing, 100050, China; National Clinical Research Center for Digestive Diseases, Beijing, 100050, China; Beijing Key Laboratory of Tolerance Induction and Organ Protection in Transplantation, Beijing, 100050, China; Immunology Research Center for Oral and Systemic Health, Beijing Friendship Hospital, Capital Medical University, China; Beijing Clinical Research Institute, Beijing, 100050, China. Electronic address:

CD4 and CD8 coreceptor double negative TCRαβ T (DNT) cells are increasingly being recognized for their critical and diverse roles in the immune system. However, their molecular and functional signatures remain poorly understood and controversial. Moreover, the majority of studies are descriptive because of the relative low frequency of cells and non-standardized definition of this lineage. In this study, we performed single-cell RNA sequencing on 28,835 single immune cells isolated from mixed splenocytes of male C57BL/6 mice using strict fluorescence-activated cell sorting. The data was replicated in a subsequent study. Our analysis revealed five transcriptionally distinct naïve DNT cell clusters, which expressed unique sets of genes and primarily performed T helper, cytotoxic and innate immune functions. Anti-CD3/CD28 activation enhanced their T helper and cytotoxic functions. Moreover, in comparison with CD4, CD8 T cells and NK cells, Ikzf2 was highly expressed by both naïve and activated cytotoxic DNT cells. In conclusion, we provide a map of the heterogeneity in naïve and active DNT cells, addresses the controversy about DNT cells, and provides potential transcription signatures of DNT cells. The landscape approach herein will eventually become more feasible through newer high throughput methods and will enable clustering data to be fed into a systems analysis approach. Thus the approach should become the "backdrop" of similar studies in the myriad murine models of autoimmunity, potentially highlighting the importance of DNT cells and other minor lineage of cells in immune homeostasis. The clear characterization of functional DNT subsets into helper DNT, cytotoxic DNT and innate DNT will help to better understand the intrinsic roles of different functional DNT subsets in the development and progression of autoimmune diseases and transplant rejection, and thereby may facilitate diagnosis and therapy.
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http://dx.doi.org/10.1016/j.jaut.2021.102653DOI Listing
July 2021

Utility of feeding jejunostomy in patients with esophageal cancer undergoing esophagectomy with a high risk of anastomotic leakage.

J Gastrointest Oncol 2021 Apr;12(2):433-445

Department of Thoracic Surgery, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China.

Background: Feeding jejunostomy is widely used for enteral nutrition (EN) after esophagectomy; however, its risks and benefits are still controversial. We aimed to evaluate the short-term and long-term outcomes of feeding jejunal tube (FJT) in patients undergoing esophagectomy for esophageal squamous cell carcinoma (ESCC) who were deemed high-risk for anastomotic leakage.

Methods: We retrospectively analyzed 716 patients who underwent esophagectomy with (FJT group, n=68) or without (control group, n=648) intraoperative placement of FJT. Propensity score matching (PSM) was used for the adjustment of confounding factors. Risk level for anastomotic leakage was determined for every patient after PSM.

Results: Patients in the FJT group were at higher risk of anastomotic leakage (14.9% 11.3%), and had a statistically non-significant increase of postoperative complications [31.3% 21.8%, odds ratio (OR) =1.139, 95% confidence interval (CI), 0.947-1.370, P=0.141] after PSM. Medical expenditure, length of postoperative hospital stay, and short-term mortality were similar between the FJT and control groups. Placement of FJT appeared to accelerate the recovery of anastomotic leakage (27.2 37.4 d, P=0.073). Patients in FJT group achieved comparable overall survival (OS) both before [hazard ratio (HR) =0.850, P=0.390] and after (HR =0.797, P=0.292) PSM.

Conclusions: FJT showed acceptable safety profile along with potential benefits for ESCC patients with a high presumed risk of anastomotic leakage. While FJT does not impact OS, placement of FJT should be considered in esophagectomy patients and tailored to individual patients based on their leak-risk profile.
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http://dx.doi.org/10.21037/jgo-21-133DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107594PMC
April 2021

A validated nomogram integrating hematological indicators to predict response to neoadjuvant therapy in esophageal squamous cell carcinoma patients.

Ann Transl Med 2021 Apr;9(8):703

Department of Thoracic Surgery, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China.

Background: The prognoses for advanced Esophageal squamous cell cancer (ESCC) was very poor. Neoadjuvant therapy was shown to improve overall survival of ESCC patients. However, there is still no effective indicator to predict the efficacy of neoadjuvant therapy. The present study intended to investigate the correlation between hematological parameters and the efficacy of neoadjuvant therapy so as to provide a reference for the prediction of cancer response to neoadjuvant therapy.

Methods: This study included 197 ESCC patients in our center from January 2010 to December 2018. Response evaluation criteria in solid tumors (RECIST) criteria were used for the treatment evaluation. The results of univariate and multivariate logistic regression analysis were used to select independent factors for construction of the prediction model. The concordance index (C-index), receiver operating characteristic (ROC) curve, and calibration curve were used to evaluate the robustness of the model, while the bootstrap method was used for internal validation.

Results: Among the 197 included ESCC patients, 94 patients achieved partial remission, 80 patients were in stable condition, and 23 patients had disease progression, 123 of whom underwent surgery. The comparisons of the dynamic hematological test results before and after treatment show that pre-PLT, pre-MONO%, post-Hb, △WBC, and the option of undergoing neoadjuvant chemoradiation were the potential predictors for the effectiveness of neoadjuvant therapy. The model in which the C-index was 0.803 (95% confidence interval: 0.742-0.864) showed good prediction performance, and still reach a C-index of 0.764 when internally validated.

Conclusions: For the neoadjuvant treatment of ESCC, hematological indexes are closely related to the efficacy of neoadjuvant therapy. The nomogram can be used to easily predict the efficacy of neoadjuvant therapy in patients.
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http://dx.doi.org/10.21037/atm-21-1628DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8106100PMC
April 2021

APOBEC3B deletion polymorphism and lung cancer risk in the southern Chinese population.

Ann Transl Med 2021 Apr;9(8):656

Department of Epidemiology, School of Medicine, Jinan University, Guangzhou, China.

Background: Approximately 80-85% of lung cancer is the non-small cell lung cancer (NSCLC) subtype, which ranks as the leading cause of cancer deaths worldwide. (A3B) was reported to be a key source of mutations in NSCLC. However, the role of the A3B deletion polymorphism in the etiology of NSCLC has not been well-documented.

Methods: A case-control study with 317 NSCLC patients and 334 healthy controls was conducted to explore the association between the A3B deletion polymorphism and the risk of NSCLC. The unconditional logistic regression model was performed to calculate the odds ratio (OR) and the 95% confidence interval (CI), and the confounding factors were adjusted, including age, gender, and smoking status, to estimate the risk. An analysis of gene-environment interactions was performed using multifactor dimensionality reduction (MDR) software.

Results: We found that the del/del genotype of A3B deletion significantly increased NSCLC risk. Compared with individuals carrying the ins/ins genotype of A3B deletion, individuals with the del/del genotype had a 2.36 times increased risk of developing NSCLC after adjusting for confounding factors (OR =2.71, 95% CI: 1.67-4.42, P<0.001). A 3-factor gene-environment (A3B deletion, gender, and smoking) interaction model was found for NSCLC (OR =4.407, 95% CI: 1.174-16.549, P=0.028).

Conclusions: We propose that the A3B deletion polymorphism can increase the risk of developing NSCLC, and their interactions with gender and smoking may contribute to the risk of NSCLC in the southern Chinese population.
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http://dx.doi.org/10.21037/atm-21-989DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8105993PMC
April 2021

Diagnostic Value of Circulating Progranulin and Its Receptor EphA2 in Predicting the Atheroma Burden in Patients with Coronary Artery Disease.

Dis Markers 2021 21;2021:6653501. Epub 2021 Apr 21.

Department of Pharmacy, Zhongshan Hospital, Fudan University, Shanghai, China.

Background: Progranulin (PGRN) and its potential receptor Eph-receptor tyrosine kinase-type A2 (EphA2) are inflammation-related molecules that present on the atherosclerotic plaques. However, the roles of circulating PGRN and EphA2 in coronary artery disease (CAD) remain unclear.

Objective: To study the clinical significance of circulating PGRN and EphA2 levels in Chinese patients undergoing coronary angiography.

Methods: Levels of circulating EphA2 fragments and PGRN were examined in 201 consecutive individuals who underwent coronary angiography for suspected CAD in our center from Jan 2020 to Oct 2020. Demographic characteristics, results of biochemical and auxiliary examinations, and other relevant information were collected. The coronary atheroma burden was quantified by the Gensini score and the existence of chronic total occlusion (CTO). Univariate analysis and multivariate logistic regression analysis were used to analyze the risk factors for acute coronary syndrome (ACS). In patients with ACS and SAP, a receiver operating characteristic (ROC) curve was generated to detect the accuracy and discriminative ability of levels of EphA2 and PGRN, the Gensini score, and cardiac injury biomarkers as surrogate endpoints for CTO.

Results: Circulating EphA2 levels were significantly higher in patients with ACS than in subjects with stable angina pectoris (SAP) or control subjects ( < 0.001). A positive linear correlation was verified between EphA2 levels and the Gensini score ( = 0.306, < 0.001), and negative correlation was detected with the left ventricular ejection fraction (LVEF) ( = -0.405, < 0.001). Both PGRN and EphA2 were positively associated with cardiac injury biomarkers (i.e., NT-proBNP, cTnT, and hs-CRP) ( < 0.05). The area under the ROC curve of PGRN and EphA2 was 0.604 and 0.686, respectively ( < 0.01).

Conclusions: Higher circulating EphA2 and PGRN levels were detected in patients with ACS than in patients with SAP. Circulating EphA2 and PGRN levels might be diagnostic factors for predicting the atheroma burden in patients with CAD.
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http://dx.doi.org/10.1155/2021/6653501DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8084646PMC
April 2021

New Thio-Compounds and Monoterpenes With Anti-inflammatory Activities From the Fungus sp. CYH26.

Front Microbiol 2021 25;12:668938. Epub 2021 Mar 25.

Key Laboratory of Chemistry and Engineering of Forest Products, State Ethnic Affairs Commission, Guangxi Key Laboratory of Chemistry and Engineering of Forest Products, Guangxi Collaborative Innovation Center for Chemistry and Engineering of Forest Products, School of Chemistry and Chemical Engineering, Guangxi University for Nationalities, Nanning, China.

Two new thio-compounds named aspergerthinol A and B ( and ) and two new monoterpenes named aspergerthinacids A and B ( and ) were isolated from the fungus sp. CYH26 from the rhizosphere soil of Decne. The structures of compounds were elucidated by spectroscopic data and quantum NMR and ECD calculations. Compounds and represented a new family of sulfur containing natural products with a 3-methyl-4-cyclopenta[b]thiophen-4-one skeleton. Compounds showed inhibitory activities against nitric oxide (NO) with IC values of 38.0, 19.8, 46.3, and 56.6 μM, respectively.
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http://dx.doi.org/10.3389/fmicb.2021.668938DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8025228PMC
March 2021

ARRDC3 as a Diagnostic and Prognostic Biomarker for Epithelial Ovarian Cancer Based on Data Mining.

Int J Gen Med 2021 22;14:967-981. Epub 2021 Mar 22.

Department of Gynecologic Oncology, Guangxi Medical University Cancer Hospital, Nanning, Guangxi, People's Republic of China.

Purpose: The dysregulation of arrestin domain containing 3 (ARRDC3) has an important effect on oncogenesis and tumor progression in many cancers, including renal cell carcinoma and breast cancer. However, the role of ARRDC3 in ovarian cancer (OC) has not been reported.

Methods: The present study explored the diagnostic and prognostic roles of ARRDC3 in ovarian cancer using GEPIA, ONCOMINE, GEO, and Kaplan-Meier Plotter databases for training and validation. Then, we conducted a stratified analysis for clinicopathological factors using Kaplan-Meier Plotter and GEPIA databases. To further explore the mechanisms, we also used the MIST database to visualize the protein-protein interaction network of ARRDC3 associated with OC. The gene-gene interaction network was visualized by GeneMANIA plugin in Cytoscape 3.8.0 software, and the associated co-expression genes of ARRDC3 were analyzed by the cBioPortal database. The 100 top co-expression genes chosen for gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were used by the DAVID website.

Results: A significant difference in ARRDC3 mRNA expression was found between OC and normal ovary tissues. ARRDC3 could potentially be implicated in the diagnosis of OC. A high ARRDC3 mRNA expression level was associated with poor overall survival and progression-free survival. However, no significance was reported in respect to post progression survival. Except for histology, which had no prognostic value for PPS in stratified analysis, stratified analysis of other factors had prognostic value for OS, PFS, and PPS. Interestingly, we found a positive correlation between ARRDC3 expression and CD8+ T cells, macrophages, neutrophils, and dendritic cells, indicating that ARRDC3 might be associated with immune infiltration of these immune cells. Co-expression genes enrichment analysis found that they were involved in the Renin-angiotensin system pathway.

Conclusion: Differentially expressed ARRDC3 might be a potential prognostic and diagnostic marker in Ovarian Cancer.
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http://dx.doi.org/10.2147/IJGM.S302012DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7997607PMC
March 2021

Propofol Protects Regulatory T Cells, Suppresses Neurotoxic Astrogliosis, and Potentiates Neurological Recovery After Ischemic Stroke.

Neurosci Bull 2021 May 20;37(5):725-728. Epub 2021 Mar 20.

Department of Anesthesiology, Beijing Chaoyang Hospital, Capital Medical University, Beijing, 100020, China.

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http://dx.doi.org/10.1007/s12264-021-00653-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8099970PMC
May 2021

Nacre-Inspired Strong and Multifunctional Soy Protein-Based Nanocomposite Materials for Easy Heat-Dissipative Mobile Phone Shell.

Nano Lett 2021 04 19;21(7):3254-3261. Epub 2021 Mar 19.

Department of Biochemistry and Molecular Biology, Michigan State University, 603 Wilson Road, East Lansing, Michigan 48824, United States.

Inspired by the hierarchically ordered "brick and mortar" (BM) architecture of natural nacre, in this study a rational assembly of boron nitride (BN) nanosheets was introduced into a mixture of trimethylolpropane triglycidyl ether (TTE) and soy protein isolate (SPI), and a strong and multifunctional SPI-based nanocomposite film with multinetwork structure was synthesized. At a low BN loading (<0.5%), the resulting multifunctional film was flexible, antiultraviolet, and nearly transparent and also displayed good thermal diffusion ability and exhibited an excellent combination of high tensile strength (36.4 MPa) and thermal conductivity (TC, 2.40 W·m·K), surpassing the performances of various types of petroleum-based plastics (displayed a tensile strength ranging from 1.9 to 21 MPa and TC ranging from 0.55-2.13 W·m·K), including nine different types of materials currently utilized for mobile phone shells, suggesting its vast potential in practical applications.
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http://dx.doi.org/10.1021/acs.nanolett.1c00542DOI Listing
April 2021

Artificial intelligence-assisted reduction in patients' waiting time for outpatient process: a retrospective cohort study.

BMC Health Serv Res 2021 Mar 17;21(1):237. Epub 2021 Mar 17.

School of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Background: Many studies suggest that patient satisfaction is significantly negatively correlated with the waiting time. A well-designed healthcare system should not keep patients waiting too long for an appointment and consultation. However, in China, patients spend notable time waiting, and the actual time spent on diagnosis and treatment in the consulting room is comparatively less.

Methods: We developed an artificial intelligence (AI)-assisted module and name it XIAO YI. It could help outpatients automatically order imaging examinations or laboratory tests based on their chief complaints. Thus, outpatients could get examined or tested before they went to see the doctor. People who saw the doctor in the traditional way were allocated to the conventional group, and those who used XIAO YI were assigned to the AI-assisted group. We conducted a retrospective cohort study from August 1, 2019 to January 31, 2020. Propensity score matching was used to balance the confounding factor between the two groups. And waiting time was defined as the time from registration to preparation for laboratory tests or imaging examinations. The total cost included the registration fee, test fee, examination fee, and drug fee. We used Wilcoxon rank-sum test to compare the differences in time and cost. The statistical significance level was set at 0.05 for two sides.

Results: Twelve thousand and three hundred forty-two visits were recruited, consisting of 6171 visits in the conventional group and 6171 visits in the AI-assisted group. The median waiting time was 0.38 (interquartile range: 0.20, 1.33) hours for the AI-assisted group compared with 1.97 (0.76, 3.48) hours for the conventional group (p < 0.05). The total cost was 335.97 (interquartile range: 244.80, 437.60) CNY (Chinese Yuan) for the AI-assisted group and 364.58 (249.70, 497.76) CNY for the conventional group (p < 0.05).

Conclusions: Using XIAO YI can significantly reduce the waiting time of patients, and thus, improve the outpatient service process of hospitals.
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http://dx.doi.org/10.1186/s12913-021-06248-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7966905PMC
March 2021

3D segmentation of lungs with juxta-pleural tumor using the improved active shape model approach.

Technol Health Care 2021 ;29(S1):385-398

College of Information and Engineering, Shenyang University, Shenyang, Liaoning, China.

Background And Objective: At present, there are many methods for pathological lung segmentation. However, there are still two unresolved problems. (1) The search steps in traditional ASM is a least square optimization method, which is sensitive to outlier marker points, and it makes the profile update to the transition area in the middle of normal lung tissue and tumor rather than a true lung contour. (2) If the noise images exist in the training dataset, the corrected shape model cannot be constructed.

Methods: To solve the first problem, we proposed a new ASM algorithm. Firstly, we detected these outlier marker points by a distance method, and then the different searching functions to the abnormal and normal marker points are applied. To solve the second problem, robust principal component analysis (RPCA) of low rank theory can remove noise, so the proposed method combines RPCA instead of PCA with ASM to solve this problem. Low rank decompose for marker points matrix of training dataset and covariance matrix of PCA will be done before segmentation using ASM.

Results: Using the proposed method to segment 122 lung images with juxta-pleural tumors of EMPIRE10 database, got the overlap rate with the gold standard as 94.5%. While the accuracy of ASM based on PCA is only 69.5%.

Conclusions: The results showed that when the noise sample is contained in the training sample set, a good segmentation result for the lungs with juxta-pleural tumors can be obtained by the ASM based on RPCA.
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http://dx.doi.org/10.3233/THC-218037DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8150541PMC
January 2021

Association between vaccinations and clinical manifestations in children with COVID-19.

Transl Pediatr 2021 Jan;10(1):17-25

Institute of Maternal and Child Health, Wuhan Children's Hospital, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, China.

Background: The infection rate of Coronavirus Disease 2019 (COVID-19) in children was less than that in adults. However, the underlining reason is not well known.

Methods: Children with COVID-19 were recruited from two Children's Hospitals in Wuhan and Shanghai in this case-control study. The associations of initial symptoms with age, vaccinations of Bacillus Calmette Guerin (BCG), and influenza and pathogens were determined by Chi-square -test.

Results: We evaluated 248 confirmed cases, and 56 suspected cases with COVID-19. The median age was 6.82 years old, and 118 cases (38.82%) were girls. Furthermore, 30.26% of all patients were asymptomatic cases. The percentage of asymptomatic cases vaccinated with BCG was not significantly higher than that without BCG vaccination [86/280 (30.71%) 6/13 (46.15%), P=0.203], and initial symptoms were not related with immunized influenza vaccine (P=0.267). Compared to parameters in pediatric patients with normal body temperatures, patients with fever had higher C reactive protein (CRP) (P<0.001).

Conclusions: Pediatric COVID-19 patients with BCG vaccinations exhibit similar clinical manifestations compared to those without BCG vaccinations, and the severity of symptoms in pediatric patients may be related to the maturity of immune function.
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http://dx.doi.org/10.21037/tp-20-225DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7882301PMC
January 2021

Synergistically coupling of Fe-doped CoP nanocubes with CoP nanosheet arrays towards enhanced and robust oxygen evolution electrocatalysis.

J Colloid Interface Sci 2021 Jun 1;591:67-75. Epub 2021 Feb 1.

Institute for Energy Research, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, China. Electronic address:

The rational design of high-performance and low-cost oxygen evolution reaction (OER) electrocatalysts for water splitting is of vital importance for development of renewable hydrogen energy. Herein, we demonstrate an interfacial engineering strategy to prepare Fe-doped CoP nanocubes/CoP nanosheet arrays heterostructure supported on carbon cloth (denoted as CoFeP/CoP/CC). The resultant CoFeP/CoP/CC heterostructure catalyst possesses abundant heterogeneous interfaces, which enables the exposure of reaction active sites and possibly modulation of electronic structure of the catalyst. Furthermore, this strong interfacial coupling of CoFeP and CoP as well as the integration structure on the carbon cloth guarantee high electronic conductivity and enhanced mechanical stability. Benefiting from these advantages, the CoFeP/CoP/CC-heterostructure exhibits high electrocatalytic OER performance with a low overpotential of 240 mV for reaching a current density of 10 mA cm, which outperforms the commercial noble metal RuO (255 mV) and many reported TMPs-based electrocatalysts. Moreover, this CoFeP/CoP/CC catalyst shows a remarkable OER catalytic stability over 100 h. This work provides an effective avenue for the design of the high-performance OER catalyst by interfacial engineering strategy.
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http://dx.doi.org/10.1016/j.jcis.2021.01.084DOI Listing
June 2021

Liraglutide monotherapy and add on therapy on obese women with polycystic ovarian syndromes: a systematic review and meta-analysis.

Minerva Med 2021 Feb 8. Epub 2021 Feb 8.

Department of Pharmacy, Zhongshan Hospital, Fudan University, Shanghai, China -

Introduction: A meta-analysis was carried out to systematically evaluate the effects of liraglutide on waist circumference, BMI, weight, insulin resistance (IR), Follicle-Stimulating Hormone (FSH), and Luteinizing hormone (LH) levels in obese women with polycystic ovarian syndromes (PCOS).

Evidence Acquisition: Randomized and non-randomized control trials of liraglutide monotherapy and add-on therapy were searched through PUBMED, Web of Science, Cochrane Library, EBSCO, CNKI (China National Knowledge Infrastructure), the Wanfang database, and Google Scholar databases up to May 30, 2020. Information about the impact of liraglutide on polycystic ovarian syndromes (PCOS) related to obese patients was extracted by two reviewers independently, and Revman 5.3 was used for meta-analysis.

Evidence Synthesis: A total of six studies with 401 women were included in this research. Waist circumference, BMI, weight was significantly reduced by -6.28 cm (95%CI = -7.89, -4.67; P < 0.00001), -2.53 kg/m2 (95%CI = -2.79, -2.27; P < 0.00001) and -4.33 kg (95%CI = -6.05, -2.61; P < 0.00001) after treatment with liraglutide monotherapy or add on therapy. There was a moderate declination in HOMA-IR scores following liraglutide treatment with -0.81 (95%CI = -0.97, -0.64; P < 0.00001). FSH and LH show a decline in the liraglutide group with high heterogeneity (I2 > 95%).

Conclusions: For women with PCOS, BMI, weight, and waist circumference were significantly decreased after 12~24 weeks of treatment with liraglutide monotherapy and add-on therapy. Insulin resistance might be improved with the use of liraglutide. Nevertheless, large scale randomized control trials should be carried out to elucidate the long-term outcome in improving the insulin sensitivity of liraglutide treatment.
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http://dx.doi.org/10.23736/S0026-4806.21.07085-3DOI Listing
February 2021

Homocysteine Impairs Endothelial Cell Barrier Function and Angiogenic Potential via the Progranulin/EphA2 Pathway.

Front Pharmacol 2020 8;11:614760. Epub 2021 Jan 8.

Department of Pharmacy, Zhongshan Hospital, Fudan University, Shanghai, China.

Hyperhomocysteinemia is a well-recognized independent risk factor for cardiovascular disease. To date, the mechanism of pathological plasma homocysteine (Hcy) level elevation remains to be elucidated. We aimed to investigate the levels of progranulin (PGRN), Eph-receptor tyrosine kinase-type A2 (EphA2), vascular cell adhesion molecule-1 (VCAM-1), and Hcy in patients with arteriosclerosis and investigate their functions in Hcy-injured human umbilical vein endothelial cells (HUVECs). EphA2 knockdown was induced in HUVECs by shRNA lentivirus infection with EphA2-RNAi, and bulk RNA-seq assay was performed. Then we investigated the mechanism underlying the effect of recombinant human PGRN (rhPGRN) combined with shRNA interference of EphA2 on cell proliferation, migration, and angiogenesis in Hcy-injured HUVECs. Results showed that serum EphA2, VCAM-1, and Hcy levels in acute coronary syndrome patients were significantly higher than those in chronic coronary syndrome patients ( = 0.000; = 0.000; = 0.033, respectively). , we demonstrated that knockdown of EphA2 significantly impaired cell adhesion and inhibited HUVECs migration and angiogenesis ( < 0.001), which was associated with reduction in VCAM1 and VE-cadherin ( < 0.05). Hcy modulated the expression of PGRN and EphA2 in a time-and dose-dependent manner. However, rhPGRN ameliorated the Hcy-induced reduction in cell viability and migration ( < 0.05). Mechanistically, we found that PGRN/EphA2 and its downstream AKT/NF-κB signaling might be the primary signal transduction pathways underlying Hcy-induced injury. The present study illustrated that PGRN plays a previously unrecognized role in Hcy-induced endothelial injury, which is achieved through its interaction with EphA2 signaling, implying a promising therapeutic target for cardiovascular disease.
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http://dx.doi.org/10.3389/fphar.2020.614760DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7836014PMC
January 2021

CD4 derived double negative T cells prevent the development and progression of nonalcoholic steatohepatitis.

Nat Commun 2021 01 28;12(1):650. Epub 2021 Jan 28.

General Surgery Department, Beijing Friendship Hospital, Capital Medical University, Beijing, China.

Hepatic inflammation is the driving force for the development and progression of NASH. Treatment targeting inflammation is believed to be beneficial. In this study, adoptive transfer of CD4 T cells converted double negative T cells (cDNT) protects mice from diet-induced liver fat accumulation, lobular inflammation and focal necrosis. cDNT selectively suppress liver-infiltrating Th17 cells and proinflammatory M1 macrophages. IL-10 secreted by M2 macrophages decreases the survival and function of cDNT to protect M2 macrophages from cDNT-mediated lysis. NKG2A, a cell inhibitory molecule, contributes to IL-10 induced apoptosis and dampened suppressive function of cDNT. In conclusion, ex vivo-generated cDNT exert potent protection in diet induced obesity, type 2 diabetes and NASH. The improvement of outcome is due to the inhibition on liver inflammatory cells. This study supports the concept and the feasibility of potentially utilizing this autologous immune cell-based therapy for the treatment of NASH.
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http://dx.doi.org/10.1038/s41467-021-20941-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7844244PMC
January 2021

MicroRNA-21 Contributes to Acute Liver Injury in LPS-Induced Sepsis Mice by Inhibiting PPAR Expression.

PPAR Res 2020 22;2020:6633022. Epub 2020 Dec 22.

Department of Critical Care Medicine, Renmin Hospital of Wuhan University, 238 Jiefang Road, Wuchang, Wuhan, Hubei 430060, China.

The severity of sepsis may be associated with excessive inflammation, thus leading to acute liver injury. MicroRNA-21 is highly expressed in the liver of a variety of inflammation-related diseases, and PPAR is also proved to participate in regulating inflammation. In the present study, the LPS-induced sepsis model was established. We found that microRNA-21 expression was upregulated in the liver of sepsis mice, and microRNA-21 inhibition significantly reduced the liver injury. The expression of liver injury markers, inflammation cytokines, and PPAR in the septic mice was higher than in antagomir-21 treated septic mice. In addition, we also found that PPAR is the target gene of microRNA-21; PPAR antagonist GW6471 could reverse the effect of antagomir-21. In conclusion, our study illustrated that microRNA-21 exacerbate acute liver injury in sepsis mice by inhibiting PPAR expression.
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http://dx.doi.org/10.1155/2020/6633022DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7772039PMC
December 2020

Association of three missense mutations in the homocysteine-related MTHFR and MTRR gene with risk of polycystic ovary syndrome in Southern Chinese women.

Reprod Biol Endocrinol 2021 Jan 7;19(1). Epub 2021 Jan 7.

Department of Gynecology, Obstetrics and Gynecology Center, Zhujiang Hospital, Southern Medical University, No.253, Gongye Middle Avenue, Haizhu District, 510280, Guangzhou, Guangdong, China.

Background: The etiology between homocysteine and polycystic ovary syndrome (PCOS) is unclear. In humans, the level of homocysteine is mainly affected by two enzymes: methylene tetrahydrofolate reductase (MTHFR) and methionine synthase reductase (MTRR). While the activity of these two enzymes is mainly affected by three missense mutations, namely C677T (MTHFR), A1298C (MTHFR), and A66G (MTRR). This study aims to examine the association between the three missense mutations and PCOS and investigate whether the three missense mutations exerted their effect on PCOS by affecting the homocysteine level.

Methods: A case-control study was designed, comprising 150 people with PCOS and 300 controls. Logistic regression analysis was used to assess the association between the three missense mutations and PCOS. Linear regression analysis was used to assess the association between the three missense mutations and the homocysteine level. Mediation analysis was used to investigate whether the three missense mutations exerted their effect on PCOS by affecting the homocysteine level.

Results: Following adjustments and multiple rounds of testing, MTHFR A1298C was found to be significantly associated with PCOS in a dose-dependent manner (compared to AA, OR = 2.142 for AC & OR = 3.755 for CC; P < 0.001). MTRR A66G was nominally associated with PCOS. Mutations in MTHFR A1298C and MTRR A66G were significantly associated with the homocysteine level. Mediation analysis suggested the effect of MTHFR A1298C on PCOS was mediated by homocysteine.

Conclusions: MTHFR A1298C and MTRR A66G were associated with PCOS, and MTHFR A1298C might affect the risk of PCOS by influencing the homocysteine level.
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http://dx.doi.org/10.1186/s12958-020-00688-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7789417PMC
January 2021

Characterization of the complete chloroplast genome of cv. Acutifolia (Apocynaceae).

Mitochondrial DNA B Resour 2020 Jan 29;5(1):927-928. Epub 2020 Jan 29.

Guangxi Botanical Garden of Medicinal Plants, Nanning, Guangxi, China.

cv. Acutifolia is a widely planted landscape tree in the subtropics. In this study, the complete chloroplast genome of cv. Acutifolia was determined through Illumina sequencing method. The complete chloroplast genome has a length of 153,912 bp, containing a small single-copy region (18,036 bp), a large single-copy region (84,852 bp), and a pair of IR regions (25,512 bp). The chloroplast genome possesses 130 genes, including 85 CDS, 37 tRNA genes and 8 rRNA genes.  cv. Acutifolia exhibited the closest relationship with in phylogenetic analysis.
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http://dx.doi.org/10.1080/23802359.2020.1721023DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7748865PMC
January 2020

Improvement of Postharvest Anthracnose Resistance in Mango Fruit by Nitric Oxide and the Possible Mechanisms Involved.

J Agric Food Chem 2020 Dec 15;68(52):15460-15467. Epub 2020 Dec 15.

School of Environmental and Safety Engineering, Changzhou University, Changzhou, Jiangsu 213164, People's Republic of China.

The anthracnose rot of postharvest mango fruit is a devastating fungal disease often resulting in tremendous quality deterioration and postharvest losses. Nitric oxide (NO), as an important signaling molecule, is involved in the responses to postharvest fruit diseases. In the present study, the effectiveness of NO donor sodium nitroprusside (SNP) to prevent anthracnose of "Tainong" mango fruit caused by was evaluated through in vivo and in vitro tests. Results from in vivo test showed that SNP treatment effectively inhibited the lesion diameter and disease incidence on inoculated mango fruit during storage. SNP treatment could regulate hydrogen peroxide levels by reinforcing the activities of catalase, peroxidase, superoxide dismutase, and ascorbate peroxidase. Furthermore, SNP elevated the accumulation of lignin, total phenolics, anthocyanin, and flavonoids and the activities of chitinase and β-1,3-glucanase. In addition, in vitro tests indicated that SNP markedly suppressed mycelial growth and spore germination of through damaging plasma membrane integrity and increasing the leakage of soluble sugar and protein. Our results suggested that SNP could suppress anthracnose decay in postharvest mango fruit, possibly by directly suppressing pathogen growth and indirectly triggering host defense responses.
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http://dx.doi.org/10.1021/acs.jafc.0c04270DOI Listing
December 2020

Inhibition of NOX4/ROS Suppresses Neuronal and Blood-Brain Barrier Injury by Attenuating Oxidative Stress After Intracerebral Hemorrhage.

Front Cell Neurosci 2020 13;14:578060. Epub 2020 Nov 13.

Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, Department of Neurosurgery, Zhujiang Hospital, The National Key Clinical Specialty, The Neurosurgery Institute of Guangdong Province, The Engineering Technology Research Center of Education Ministry of China, Southern Medical University, Guangzhou, China.

Intracerebral hemorrhage (ICH) is a common and severe neurological disorder that can effectively induce oxidative stress responses. NADPH oxidase 4 (NOX4) is a member of the NOX family of oxidases. It is expressed in the brain normally and involved in cell signal transduction and the removal of harmful substances. In some pathological conditions, it mediates inflammation and the aging of cells. However, few studies have focused on whether NOX4 is involved in brain injury caused by ICH. Therefore, this study aimed to clarify the role of NOX4 in the pathological process that occurs after ICH and the potential mechanism underlying its role. A rat model of ICH was established by the injection of collagenase type IV, and the expression of NOX4 was then determined. Further, siRNA-mediated protein expression knockdown technology was used for NOX4 knockdown, and western immunoblotting, immunohistochemistry, immunofluorescence, enzyme-linked immunosorbent assay (ELISA), and other molecular biological techniques were performed to assess the effects of NOX4 knockdown. Neurobiological scoring, brain water content determination, and other brain injury detection methods were also performed to assess the role of NOX4 following ICH. We found that the expression of NOX4 increased in the brains of rats after ICH, and that it was mainly expressed in neurons, astrocytes, vascular endothelial cells and microglia. Following NOX4 knockdown, the level of oxidative stress in the brain decreased considerably, the neurobehavioral scores improved, the levels of neuronal apoptosis reduced markedly, and the impairment of blood-brain barrier function was significantly ameliorated in rats with ICH. In conclusion, this study suggests that NOX4 expression is upregulated after ICH, which may cause an imbalance in the oxidative stress of relevant cells in the brain, leading to subsequent apoptosis of neurons and damage to the blood-brain barrier due to secondary brain injury following ICH.
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http://dx.doi.org/10.3389/fncel.2020.578060DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7691600PMC
November 2020
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