Publications by authors named "Dan Song"

317 Publications

sp. nov., isolated from Indian Ocean marine sediment.

Int J Syst Evol Microbiol 2021 Jul;71(7)

State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR China.

A novel Gram-stain-positive, catalase-positive, oxidase-negative, aerobic, non-motile, rod-shaped bacterium, designated strain YIM M12148, was isolated from a marine sediment sample collected from the Indian Ocean. The strain grew optimally at 28 °C, pH 8.0 and in the presence of 1-3 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM M12148 belongs to the genus , with the highest sequence similarity to NBRC 15706 (96.12 %). The cell-wall sugars of strain YIM M12148 were rhamnose, ribose, glucose and mannose. The predominant isoprenoid quinones were MK-8 and MK-9. The polar lipids consisted of major amounts of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unknown phospholipid and one unknown lipid. Major fatty acids (>5 % of the total) of the novel isolate were anteiso-C, iso-C, iso-C and anteiso-C. The genomic DNA G+C content of strain YIM M12148 was 67.15 mol%. On the basis of genotypic and phenotypic data, it is apparent that strain YIM M12148 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is YIM M12148 (=KCTC 29660=DSM 29154).
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http://dx.doi.org/10.1099/ijsem.0.004906DOI Listing
July 2021

Hepatoprotective Effects of Against Carbon Tetrachloride-Induced Acute Liver Injury in Rats.

Dose Response 2021 Jul-Sep;19(3):15593258211029510. Epub 2021 Jul 12.

Key Laboratory for Molecular Genetic Mechanisms and Intervention Research on High Altitude Disease of Tibet Autonomous Region, School of Medicine, Xizang Minzu University, Xianyang, Shaanxi, China.

Purpose: To determine the hepatoprotective mechanisms of in rats with acute liver injury induced by carbon tetrachloride (CCl).

Methods: Rats were intragastrically administered twice a day for 14 consecutive days and were intraperitoneally challenged with CCl. Alanine aminotransferase and aspartate aminotransferase were measured to indicate liver injury. Malondialdehyde antioxidant enzyme activity and tumor necrosis factor-alpha and interleukin 6 secretion were measured as liver injury indicators. Histopathological tests were conducted to determine whether ameliorated liver injury.

Results: CCl-induced liver injury led to significant increases in liver injury biochemical indicators transaminase and malondialdehyde activities. pretreatments inhibited these increases. Pathological sections in pretreated samples exhibited reduced vacuole formation, neutrophil infiltration, and necrosis.

Conclusion: increases the antioxidant capacity of the liver and maintains hepatocyte function in the face of CCl-induced injury.
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http://dx.doi.org/10.1177/15593258211029510DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8278464PMC
July 2021

Rapid, label-free, and sensitive point-of-care testing of anti-SARS-CoV-2 IgM/IgG using all-fiber Fresnel reflection microfluidic biosensor.

Mikrochim Acta 2021 07 19;188(8):261. Epub 2021 Jul 19.

School of Environment and Natural Resources, Renmin University of China, Beijing, 100872, China.

The ongoing global pandemic of SARS-CoV-2 has promoted to develop novel serological testing technologies since they can be effectively complementary to RT-PCR. Here, a new all-fiber Fresnel reflection microfluidic biosensor (FRMB) was constructed through combining all-fiber optical system, microfluidic chip, and multimode fiber bio-probe. The transmission of the incident light and the collection and transmission of Fresnel reflection light are achieved using a single-multi-mode fiber optic coupler (SMFC) without any other optical separation elements. This compact design greatly simplifies the whole system structure and improves light transmission efficiency, which makes it suitable for the label-free, sensitive, and easy-to-use point-of-care testing (POCT) of targets in nanoliter samples. Based on Fresnel reflection mechanism and immunoassay principle, both the SARS-CoV-2 IgM and IgG antibodies against the SARS-CoV-2 spike protein could be sensitively quantified in 7 min using the secondary antibodies-modified multimode fiber bio-probe. The FRMB performs in one-step, is accurate, label-free, and sensitive in situ/on-site detection of SARS-CoV-2 IgM or IgG in serum with simple dilution only. The limits of detection of SARS-CoV-2 IgM and SARS-CoV-2 IgG were 0.82 ng/mL and 0.45 ng/mL, respectively. Based on our proposed theory, the affinity constants of SARS-CoV-2 IgM or IgG antibody and their respective secondary antibodies were also determined. The FRMB can be readily extended as a universal platform for the label-free, rapid, and sensitive in situ/on-site measurement of other biomarkers and the investigation of biomolecular interaction.
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http://dx.doi.org/10.1007/s00604-021-04911-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8286882PMC
July 2021

Sleep disturbance mediates the relationship between depressive symptoms and cognitive function in older adults with mild cognitive impairment.

Geriatr Nurs 2021 Jul 10;42(5):1019-1023. Epub 2021 Jul 10.

Shenzhen Qianhai Shekou Free Trade Zone Hospital, No.36, 7th Industrial Road, Nanshan District, Shenzhen City, Guangdong province, 518067, China. Electronic address:

This study examined whether and to what extent sleep disturbance mediates the effects of depressive symptoms on the cognition of individuals with mild cognitive impairment (MCI), who represent a high-risk group for developing dementia. Cross-sectional data were obtained from a sample of 204 Chinese community-dwelling older adults with MCI. MCI subjects were screened using the Montreal Cognitive Assessment, sleep quality was measured using the Pittsburgh Sleep Quality Index, and depressive symptoms were assessed using the Geriatric Depression Scale. Mediation analysis was conducted using the PROCESS macro with 10,000 bootstrap samples. The significant mediating effect of sleep quality on the association between depressive symptoms and cognition (Beta = -0.025; 95% CI, -0.054 to -0.007) explains 26% of the total effect of depressive symptoms on cognition and implies that the timely detection and management of sleep disturbance among the MCI population is highly important, especially for those with depressive symptoms.
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http://dx.doi.org/10.1016/j.gerinurse.2021.06.004DOI Listing
July 2021

Coenzyme Q10 ameliorates BPA-induced apoptosis by regulating autophagy-related lysosomal pathways.

Ecotoxicol Environ Saf 2021 Sep 26;221:112450. Epub 2021 Jun 26.

Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Zhejiang Provincial Engineering Laboratory for Animal Health and Internet Technology, College of Animal Science and Technology, Zhejiang A&F University, Hangzhou 311300, China. Electronic address:

Bisphenol A (BPA) is a widely distributed environmental endocrine disruptor. The accumulation of BPA has been proved that produce various toxic effects both on human and animals. However, the strategies to reduce the damage of BPA on the body and related mechanisms remain to be studied. Coenzyme Q10 (CoQ10), as a powerful antioxidant, is ubiquitous in many eukaryotic cells, which can improve the integrity of lysosomal membrane, lysosomal degradation function and promote autophagy. Here, we examined the ability of CoQ10 to alleviate oxidative stress and apoptosis in BPA-induced damages in C2C12 cells, and how to alleviate it. Our results showed that BPA treatment significantly reduced cell viability, increased the number of cell apoptosis and ROS production, decreased mitochondrial membrane potential, and inhibited the gene expression of mitochondria biogenesis. Moreover, we demonstrated that exposure to BPA increased expression levels of autophagy protein (LC3-II, p62), inhibited autophagy flux, and disrupted the acidic pH environment of lysosomes. Importantly, CoQ10 supplementation effectively restored these abnormalities caused by BPA. CoQ10 significantly decreased the apoptotic incidence and ROS levels, improved mitochondrial membrane potential. Moreover, CoQ10 improved lysosome function and enhanced autophagy flux. Taken together, our results indicate that CoQ10 supplementation is a feasible and effective way to promote the level of autophagy by improving lysosomal function, thereby reducing the apoptosis caused by BPA accumulation. This study aims to provide evidence for the role of CoQ10 in repairing BPA-induced cell damage in clinical practice.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112450DOI Listing
September 2021

Application of fluorescent dextrans to the brain surface under constant pressure reveals AQP4-independent solute uptake.

J Gen Physiol 2021 Aug 15;153(8). Epub 2021 Jun 15.

Departments of Medicine and Physiology, University of California San Francisco, San Francisco, CA.

Extracellular solutes in the central nervous system are exchanged between the interstitial fluid, the perivascular compartment, and the cerebrospinal fluid (CSF). The "glymphatic" mechanism proposes that the astrocyte water channel aquaporin-4 (AQP4) is a major determinant of solute transport between the CSF and the interstitial space; however, this is controversial in part because of wide variance in experimental data on interstitial uptake of cisternally injected solutes. Here, we investigated the determinants of solute uptake in brain parenchyma following cisternal injection and reexamined the role of AQP4 using a novel constant-pressure method. In mice, increased cisternal injection rate, which modestly increased intracranial pressure, remarkably increased solute dispersion in the subarachnoid space and uptake in the cortical perivascular compartment. To investigate the role of AQP4 in the absence of confounding variations in pressure and CSF solute concentration over time and space, solutes were applied directly onto the brain surface after durotomy under constant external pressure. Pressure elevation increased solute penetration into the perivascular compartment but had little effect on parenchymal solute uptake. Solute penetration and uptake did not differ significantly between wild-type and AQP4 knockout mice. Our results offer an explanation for the variability in cisternal injection studies and indicate AQP4-independent solute transfer from the CSF to the interstitial space in mouse brain.
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http://dx.doi.org/10.1085/jgp.202112898DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8210582PMC
August 2021

BAFF signaling drives interstitial transformation of mouse renal tubular epithelial cells in a Pin1-dependent manner.

In Vitro Cell Dev Biol Anim 2021 Jun 14. Epub 2021 Jun 14.

Urology Department, Third Affiliated Hospital of Soochow University, 185# Juqian Street, Changzhou, 213003, China.

Aberrant expression of B cell-activating factor belonging to TNF superfamily (BAFF) and its receptors results in abnormal biological activities in hematopoietic and non-hematopoietic cells and is closely associated with the occurrence and development of various diseases. However, the biological significance and potential mechanisms underlying BAFF signaling in renal tubular epithelial cells (RTECs) remain unknown. This study aimed to investigate the biological role of BAFF signaling in RTECs. Mice primary RTECs were applied. The proliferation status and apoptotic rates were examined by MTS assay and flow cytometry, respectively. The expression of BAFF and its receptors was analyzed via flow cytometry and sodium ion transport function, and cytokeratin-18 expression was detected through immunofluorescence staining. In addition, Pin1 was knocked down via siRNA and its expression was assessed through reverse transcription PCR. Lastly, western blotting was performed to analyze E-cadherin, ɑ-SMA, and Pin1 expression. Results suggested that BAFF-R was significantly upregulated upon IFN-γ stimulation, and enhancement of BAFF signaling promoted cell survival and reduced their apoptotic rate, while simultaneously reducing the epithelial phenotype and promoting the interstitial transformation of cells. Furthermore, Pin1 was significantly increased, along with the upregulation of BAFF signaling in the RTECs, and participated in interstitial transformation induced by BAFF signaling. Collectively, the present results elucidate the potential mechanism of loss of normal function of RTECs under long-term high dose of BAFF stimulation provides a potential therapeutic target for renal interstitial fibrosis, and underlining mechanisms of shortening of long-term outcomes of kidney allografts via augmenting of BAFF signaling.
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http://dx.doi.org/10.1007/s11626-021-00598-yDOI Listing
June 2021

Hypertrophic cardiomyopathy β-cardiac myosin mutation (P710R) leads to hypercontractility by disrupting super relaxed state.

Proc Natl Acad Sci U S A 2021 Jun;118(24)

Department of Pediatrics (Cardiology), Stanford University School of Medicine, Palo Alto, CA 94304;

Hypertrophic cardiomyopathy (HCM) is the most common inherited form of heart disease, associated with over 1,000 mutations, many in β-cardiac myosin (MYH7). Molecular studies of myosin with different HCM mutations have revealed a diversity of effects on ATPase and load-sensitive rate of detachment from actin. It has been difficult to predict how such diverse molecular effects combine to influence forces at the cellular level and further influence cellular phenotypes. This study focused on the P710R mutation that dramatically decreased in vitro motility velocity and actin-activated ATPase, in contrast to other MYH7 mutations. Optical trap measurements of single myosin molecules revealed that this mutation reduced the step size of the myosin motor and the load sensitivity of the actin detachment rate. Conversely, this mutation destabilized the super relaxed state in longer, two-headed myosin constructs, freeing more heads to generate force. Micropatterned human induced pluripotent derived stem cell (hiPSC)-cardiomyocytes CRISPR-edited with the P710R mutation produced significantly increased force (measured by traction force microscopy) compared with isogenic control cells. The P710R mutation also caused cardiomyocyte hypertrophy and cytoskeletal remodeling as measured by immunostaining and electron microscopy. Cellular hypertrophy was prevented in the P710R cells by inhibition of ERK or Akt. Finally, we used a computational model that integrated the measured molecular changes to predict the measured traction forces. These results confirm a key role for regulation of the super relaxed state in driving hypercontractility in HCM with the P710R mutation and demonstrate the value of a multiscale approach in revealing key mechanisms of disease.
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http://dx.doi.org/10.1073/pnas.2025030118DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8214707PMC
June 2021

Fluorescence enhancement of CdSe/ZnS quantum dots induced by mercury ions and its applications to the on-site sensitive detection of mercury ions.

Mikrochim Acta 2021 May 29;188(6):215. Epub 2021 May 29.

School of Environment and Natural Resources, Renmin University of China, Beijing, 100872, China.

The significant fluorescence enhancement of CdSe/ZnS quantum dots (QDs) induced by Hg was observed for the first time based on a CdSe/ZnS QD-modified fiber nanoprobe. The fluorescence enhancement mechanism contributed to the Zn-to-Hg cation exchange in the ZnS shell, which allowed to form a HgZnS/CdSe heterojunction and increase the separation of electrons and holes and reduce the recombination rate. High concentrations of Hg accelerated the generation of the fluorescence signal and lead to higher fluorescence intensity. The maximum fluorescence intensity increased more than eight times when Hg concentration was 1 µM. The characteristic time (θ), i.e., the rising time to achieve the maximum fluorescence intensity, was linearly dependent on  initial concentration of Hg solution in accordance with our proposed theory. When the evanescent wave optofluidic fluorescence platform was used, the linear detection range and detection limit of Hg were 5.0-1000 nM and 0.80 nM, respectively. The fiber nanoprobe can be applied to the rapid, sensitive, and accurate on-site detection of Hg in real water samples without significant matrix effect. Our work paves a novel way to develop a simple and reliable nanoprobe for mercuric pollution control, and achieve the high quantum efficiency of QDs by limiting the diffusion of Hg in the ZnS shell.
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http://dx.doi.org/10.1007/s00604-021-04871-5DOI Listing
May 2021

Regioselective C-H dithiocarbamation of indolizines with tetraalkylthiuram disulfide under metal-free conditions.

Org Biomol Chem 2021 Jun 28;19(24):5284-5288. Epub 2021 May 28.

School of Chemistry and Chemical Engineering and Guangdong Cosmetics Engineering & Technology Research Center, Guangdong Pharmaceutical University, Zhongshan 528458, China.

An efficient and straightforward metal-free regioselective C-H dithiocarbamation of indolizines with tetraalkylthiuram disulfide has been described. A series of indolizine-dithiocarbamate derivatives were easily accessed in moderate to good yields with a broad scope. In addition, imidazo[1,2-a]pyridines were also well tolerated to afford diverse imidazoheterocycle-dithiocarbamate products, which are expected to be utilized for drug discovery. Of note, the reaction could be readily scaled up, and shows its practical value in organic synthesis.
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http://dx.doi.org/10.1039/d1ob00701gDOI Listing
June 2021

Sensitizing the Luminescence of Lanthanide-Doped Nanoparticles over 1500 nm for High-Contrast and Deep Imaging of Brain Injury.

Anal Chem 2021 06 25;93(22):7949-7957. Epub 2021 May 25.

College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.

Real-time and in situ visualization of cerebrovascular dysfunction is significant for studying brain injury, which however, is restricted by the complex brain structure and limited imaging strategies. Luminescence imaging in NIR-IIb region (1500-1700 nm) is a promising tool owing to its merits including deep penetration, high resolution, and fast data acquisition. Unfortunately, a luminescent material in this region with sufficient brightness and biocompatibility is scarce. Herein, AgSe quantum dot-sensitized lanthanide-doped nanocrystals (QDs-LnNCs) with emission beyond 1500 nm were fabricated to image the cerebrovascular structure and hemodynamics in ischemic stroke and traumatic brain injury. The sensitization by QDs provided an over 100-fold enhanced brightness of LnNCs and a remarkable penetration depth of 11 mm. Dynamic information of blood perfusion and flow rates were acquired and the damage of the blood-brain barrier in the two injury models was investigated. Our results proved QDs-LnNCs as a kind of competent nanomaterial for noninvasive brain imaging.
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http://dx.doi.org/10.1021/acs.analchem.1c00731DOI Listing
June 2021

Laparoscopic gastrectomy for elderly gastric-cancer patients: comparisons with laparoscopic gastrectomy in non-elderly patients and open gastrectomy in the elderly.

Gastroenterol Rep (Oxf) 2021 Apr 10;9(2):146-153. Epub 2020 Sep 10.

Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi'an, Shanxi, P. R. China.

Background: The benefits of laparoscopic gastrectomy (LG) in elderly gastric-cancer patients still remain unclear. The purpose of this study was to evaluate the feasibility and safety of LG in elderly gastric-cancer patients.

Methods: We retrospectively evaluated patients who underwent LG or open gastrectomy (OG) between June 2009 and July 2015 in a single high-volume center. We compared surgical, short-term, and long-term survival outcomes among an elderly (≥70 years old) LG (ELG) group (=114), a non-elderly (<70 years old) LG (NLG) group (=740), and an elderly OG (EOG) group (=383).

Results: Except for extended time to first flatus, the surgical and short-term outcomes of the ELG group were similar to those of the NLG group. The ELG group revealed comparable disease-specific survival (DSS) rates to the NLG group (64.9% vs 66.2%, =0.476), although the overall survival (OS) rate was lower (57.0% vs 65.5%, <0.001) in the ELG group than in the NLG group. The ELG group showed longer operation time than the EOG group (236.4 ± 77.3 vs 179 ± 52.2 min, <0.001). The ELG group had less estimated blood loss (174.0 ± 88.4 vs 209.3 ± 133.8, =0.008) and shorter post-operative hospital stay (8.3 ± 2.5 vs 9.2 ± 4.5, =0.048) than the EOG group. The severity of complications was similar between the ELG and NLG groups. Multivariate analysis confirmed that LG was not a risk factor for post-operative complications.

Conclusions: LG is a feasible and safe procedure for elderly patients with acceptable short- and long-term survival outcomes.
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http://dx.doi.org/10.1093/gastro/goaa041DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8128003PMC
April 2021

Genomic inbreeding and population structure of northern pike () in Xinjiang, China.

Ecol Evol 2021 May 6;11(10):5657-5668. Epub 2021 Apr 6.

Heilongjiang River Fisheries Research Institute Chinese Academy of Fishery Sciences Harbin China.

Northern pike () was widely distributed in the high latitudes of the northern hemisphere. In China, northern pike was originally distributed only in the upper reaches of the Irtysh River in Xinjiang and has appeared in many water bodies outside the Irtysh River Basin in Northern Xinjiang. A total of four populations were collected from north to south in Xinjiang, including Irtysh River (RIR), Ulungu Lake (LUL), a small lake nearby Ulungu River (LJD), and Bosten Lake (LBO). We estimated population genomic parameters, performed gene flow analysis, and estimated the effective population size of each population. The proportion of individuals with high inbreeding coefficient ( ≥ 0.0625) accounted for 36.4% (44/121) of all sequenced individuals, approximately 4.5% (1/22) in LUL, 25.9% (7/27) in LBO, 42.9% (18/42) in RIR, and 60% (18/30) in LJD. RIR had the highest mean of genomic relatedness (coancestry coefficient = 0.025 ± 0.040, IBD = 0.036 ± 0.078). Gene flow results showed that the population spreading was from RIR into two branches, one was LBO, and the other continued to split into LUL and LJD, and migration signal from LBO to LUL was detected. Our results suggested that the extinction risk of northern pike was very low in Xinjiang of China, and the controlled capture fishery of northern pike could be developed reasonably.
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http://dx.doi.org/10.1002/ece3.7469DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8131772PMC
May 2021

IL-26 mediates epidermal growth factor receptor-tyrosine kinase inhibitor resistance through endoplasmic reticulum stress signaling pathway in triple-negative breast cancer cells.

Cell Death Dis 2021 May 21;12(6):520. Epub 2021 May 21.

Department of Therapy Development and Innovation for Immune Disorders and Cancers, Graduate School of Medicine, Juntendo University, 2-1-1, Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan.

Triple-negative breast cancer (TNBC) has a poor prognosis compared to other breast cancer subtypes. Although epidermal growth factor receptor (EGFR) is overexpressed in TNBC, clinical trials with EGFR inhibitors including tyrosine kinase inhibitors (EGFR-TKI) in TNBC have heretofore been unsuccessful. To develop effective EGFR-targeted therapy for TNBC, the precise mechanisms of EGFR-TKI resistance in TNBC need to be elucidated. In this study, to understand the molecular mechanisms involved in the differences in EGFR-TKI efficacy on TNBC between human and mouse, we focused on the effect of IL-26, which is absent in mice. In vitro analysis showed that IL-26 activated AKT and JNK signaling of bypass pathway of EGFR-TKI in both murine and human TNBC cells. We next investigated the mechanisms involved in IL-26-mediated EGFR-TKI resistance in TNBC. We identified EphA3 as a novel functional receptor for IL-26 in TNBC. IL-26 induced dephosphorylation and downmodulation of EphA3 in TNBC, which resulted in increased phosphorylation of AKT and JNK against EGFR-TKI-induced endoplasmic reticulum (ER) stress, leading to tumor growth. Meanwhile, the blockade of IL-26 overcame EGFR-TKI resistance in TNBC. Since the gene encoding IL-26 is absent in mice, we utilized human IL-26 transgenic (hIL-26Tg) mice as a tumor-bearing murine model to characterize the role of IL-26 in the differential effect of EGFR-TKI in human and mice and to confirm our in vitro findings. Our findings indicate that IL-26 activates the bypass pathway of EGFR-TKI, while blockade of IL-26 overcomes EGFR-TKI resistance in TNBC via enhancement of ER stress signaling. Our work provides novel insights into the mechanisms of EGFR-TKI resistance in TNBC via interaction of IL-26 with its newly identified receptor EphA3, while also suggesting IL-26 as a possible therapeutic target in TNBC.
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http://dx.doi.org/10.1038/s41419-021-03787-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8139965PMC
May 2021

HSP90-dependent PUS7 overexpression facilitates the metastasis of colorectal cancer cells by regulating LASP1 abundance.

J Exp Clin Cancer Res 2021 May 14;40(1):170. Epub 2021 May 14.

State key Laboratory of Cancer Biology, National Clinical Research Center for Digestive Diseases and Xijing Hospital of Digestive Diseases, Fourth Military Medical University, 710032, Xi'an, Shaanxi Province, China.

Background: Pseudouridine synthase (PUS) 7 is a member of the PUS family that catalyses pseudouridine formation. It has been shown to be involved in intellectual development and haematological malignancies. Nevertheless, the role and the underlying molecular mechanisms of PUS7 in solid tumours, such as colorectal cancer (CRC), remain unexplored. This study elucidated, for the first time, the role of PUS7 in CRC cell metastasis and the underlying mechanisms.

Methods: We conducted immunohistochemistry, qPCR, and western blotting to quantify the expression of PUS7 in CRC tissues as well as cell lines. Besides, diverse in vivo and in vitro functional tests were employed to establish the function of PUS7 in CRC. RNA-seq and proteome profiling analysis were also applied to identify the targets of PUS7. PUS7-interacting proteins were further uncovered using immunoprecipitation and mass spectrometry.

Results: Overexpression of PUS7 was observed in CRC tissues and was linked to advanced clinical stages and shorter overall survival. PUS7 silencing effectively repressed CRC cell metastasis, while its upregulation promoted metastasis, independently of the PUS7 catalytic activity. LASP1 was identified as a downstream effector of PUS7. Forced LASP1 expression abolished the metastasis suppression triggered by PUS7 silencing. Furthermore, HSP90 was identified as a client protein of PUS7, associated with the increased PUS7 abundance in CRC. NMS-E973, a specific HSP90 inhibitor, also showed higher anti-metastatic activity when combined with PUS7 repression. Importantly, in line with these results, in human CRC tissues, the expression of PUS7 was positively linked to the expression of HSP90 and LASP1, and patients co-expressing HSP90/PUS7/LASP1 showed a worse prognosis.

Conclusions: The HSP90-dependent PUS7 upregulation promotes CRC cell metastasis via the regulation of LASP1. Thus, targeting the HSP90/PUS7/LASP1 axis may be a novel approach for the treatment of CRC.
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http://dx.doi.org/10.1186/s13046-021-01951-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8120699PMC
May 2021

Simultaneous and sensitive determination of Escherichia coli O157:H7 and Salmonella Typhimurium using evanescent wave dual-color fluorescence aptasensor based on micro/nano size effect.

Biosens Bioelectron 2021 Aug 30;185:113288. Epub 2021 Apr 30.

School of Environment and Natural Resources, Renmin University of China, Beijing, 100874, China. Electronic address:

The simultaneous and sensitive determination of two common pathogenic bacteria, Escherichia coli O157:H7 (E. coli O157:H7) and Salmonella Typhimurium (S. Typhimurium) was achieved using evanescent wave dual-color fluorescence aptasensor and the fiber nanoprobe through combining the micro/nano size and time-resolved effect. Two fluorescence labeled aptasensors, Cy3-apt-E and Cy5.5-apt-S, were regarded as biorecognition elements and signal reporters for E. coli O157:H7 and S. Typhimurium, which were alternatively excited by evanescent waves originated from 520 nm to 635 nm excitation lights, respectively. The fiber nanoprobe with in-situ etched nanopores was used for distinguishing free aptasensors and aptasensors bound to pathogenic bacteria based on the limited penetrated depth of evanescent wave and the significant size difference of bacteria and nanopore. The E. coli O157:H7 and S. Typhimurium were directly and simultaneously quantitated in less than 35 min without the requirement of the complex immobilization of biorecognition molecules and bacteria enrichment/separation processes. The limits of detection of E. coli O157:H7 and S. Typhimurium were 340 CFU/mL and 180 CFU/mL, respectively. The satisfied recovery rate of real samples testing verified the feasibility and accuracy of the proposed method. Our strategy not only greatly simplifies the detection and identification process of multiple pathogenic bacteria, but also is easy to extend as a universal technology for sensitive determination of other bacteria using their respective biorecognition molecules.
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http://dx.doi.org/10.1016/j.bios.2021.113288DOI Listing
August 2021

Corrigendum: Dual NDP52 Function in Persistent CSFV Infection.

Front Microbiol 2021 12;12:673468. Epub 2021 Apr 12.

College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.

[This corrects the article DOI: 10.3389/fmicb.2019.02962.].
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http://dx.doi.org/10.3389/fmicb.2021.673468DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8072382PMC
April 2021

Dexmedetomidine protects SH-SY5Y cells against MPP -induced declining of mitochondrial membrane potential and cell cycle deficits.

Eur J Neurosci 2021 Apr 27. Epub 2021 Apr 27.

Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Dexmedetomidine (Dex), an adrenergic α2 receptor agonist, is commonly used in deep-brain stimulation surgery for Parkinson's disease (PD). However, there is evidence that the use of anaesthetics may accelerate the progression of neurodegenerative diseases. The effect of Dex on PD remains unclear. Here, we cultured the all-trans-retinoicacid (ATRA) differentiated SH-SY5Y cells in vitro and then treated with MPP (1.5mM) with or without Dex (10nM) or Dex combined with Atipamezole (Ati,100nM, adrenergic α2 receptor inhibitor). The ratio of apoptotic cells, mitochondrial membrane potential (Δψm), reactive oxygen species (ROS), cell cycle and apoptotic markers (Cleaved caspase-3, 9) were analysed by flow cytometry and immunofluorescence. We found that the levels of apoptotic ratio and cleaved caspase-3, 9 increased, ROS accumulated, and mitochondrial membrane potential decreased after MPP+treatment, while these changes were partially reversed by Dex. Dex also prevented MPP induced cell arrest by increasing G1 phase cells, decreasing S phase cells, and decreasing the expression of cyclinD1 and Cdk4. Moreover the effects of Dex were partially reversed by Ati. These findings reveal that Dex attenuated MPP -induced apoptosis of SH-SY5Y cells by preventing the loss of Δψm, reducing ROS, and regulating the cell cycle. Our findings indicated that Dex is more likely to be a potential drug for the treatment of PD.
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http://dx.doi.org/10.1111/ejn.15252DOI Listing
April 2021

Clinical characteristics and outcomes of infective endocarditis: impact of haemodialysis status, especially vascular access infection on short-term mortality.

Infect Dis (Lond) 2021 Sep 26;53(9):669-677. Epub 2021 Apr 26.

Department of Internal Medicine, Division of Cardiology, Soonchunhyang University Seoul Hospital, Seoul, Republic of Korea.

Background: Patients on haemodialysis (HD) are at high risk of infective endocarditis (IE). Research comparing the microbiological features as well as clinical characteristics and outcomes of HD and non-HD patients with IE is limited. Specifically, no data focussed on vascular access infections (VAIs) have been reported.

Methods: The medical records of patients with IE were retrospectively reviewed from January 2010 to February 2020 in a referral hospital in Korea. Those with definite or possible IE by modified Duke criteria were included in the study. The clinical characteristics, microbiological features, echocardiographic findings and outcomes of the patients were analysed.

Results: Of the 80 patients with IE, 34 had undergone HD and 46 had not. HD patients with IE had a higher in-hospital mortality rate (50% vs. 17.4%,  = .004) than non-HD patients. In multivariable stepwise Cox proportional hazards regression analysis, HD (hazard ratio = 2.633; 95% confidential interval: 1.053-6.582;  = .038) was predictors of 60-day mortality in IE patients. In HD patients, the presence of VAI was associated with a high in-hospital mortality rate (70.59% vs. 29.41%,  = .039) and all of the patients with VAIs (100%) had methicillin-resistant (MRSA) as a causative pathogen.

Conclusions: HD patients with IE showed high in-hospital mortality. HD, high C-reactive protein levels and lower left ventricular ejection fraction were predictors of 60-day mortality in IE patients. In particular, HD patients with VAIs had higher mortality rates and MRSA should be considered as the causative microorganism.
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http://dx.doi.org/10.1080/23744235.2021.1916587DOI Listing
September 2021

Applications of brain organoids in neurodevelopment and neurological diseases.

J Biomed Sci 2021 Apr 22;28(1):30. Epub 2021 Apr 22.

Department of Neurosurgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, 150086, China.

A brain organoid is a self-organizing three-dimensional tissue derived from human embryonic stem cells or pluripotent stem cells and is able to simulate the architecture and functionality of the human brain. Brain organoid generation methods are abundant and continue to improve, and now, an in vivo vascularized brain organoid has been encouragingly reported. The combination of brain organoids with immune-staining and single-cell sequencing technology facilitates our understanding of brain organoids, including the structural organization and the diversity of cell types. Recent publications have reported that brain organoids can mimic the dynamic spatiotemporal process of early brain development, model various human brain disorders, and serve as an effective preclinical platform to test and guide personalized treatment. In this review, we introduce the current state of brain organoid differentiation strategies, summarize current progress and applications in the medical domain, and discuss the challenges and prospects of this promising technology.
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http://dx.doi.org/10.1186/s12929-021-00728-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8063318PMC
April 2021

DAN: Deep-Attention Network for 3D Shape Recognition.

IEEE Trans Image Process 2021 21;30:4371-4383. Epub 2021 Apr 21.

Due to the wide applications in a rapidly increasing number of different fields, 3D shape recognition has become a hot topic in the computer vision field. Many approaches have been proposed in recent years. However, there remain huge challenges in two aspects: exploring the effective representation of 3D shapes and reducing the redundant complexity of 3D shapes. In this paper, we propose a novel deep-attention network (DAN) for 3D shape representation based on multiview information. More specifically, we introduce the attention mechanism to construct a deep multiattention network that has advantages in two aspects: 1) information selection, in which DAN utilizes the self-attention mechanism to update the feature vector of each view, effectively reducing the redundant information, and 2) information fusion, in which DAN applies attention mechanism that can save more effective information by considering the correlations among views. Meanwhile, deep network structure can fully consider the correlations to continuously fuse effective information. To validate the effectiveness of our proposed method, we conduct experiments on the public 3D shape datasets: ModelNet40, ModelNet10, and ShapeNetCore55. Experimental results and comparison with state-of-the-art methods demonstrate the superiority of our proposed method. Code is released on https://github.com/RiDang/DANN.
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http://dx.doi.org/10.1109/TIP.2021.3071687DOI Listing
April 2021

Impact of Laparoscopic Converted to Open Gastrectomy on Short- and Long-Term Outcomes of Patients with Locally Advanced Gastric Cancer: A Propensity Score-Matched Analysis.

J Gastrointest Surg 2021 Apr 5. Epub 2021 Apr 5.

Department of Surgery, Xijing Hospital of Digestive Diseases, Fourth Military Medical University, No. 127 Changle West Road, 710032, Xi'an, China.

Background: It remains unclear whether laparoscopic conversation to open gastrectomy causes higher morbidity and has an adverse effect on the long-term survival outcomes of patients with gastric cancer. This study was designed to evaluate the impact of the conversion on short and long-term outcomes of patients with locally advanced gastric cancer (AGC).

Methods: We retrospectively investigated 871 patients who initially underwent laparoscopic gastrectomy (LG) for pathologically confirmed diagnosis of AGC between February 2009 and April 2018. The patients were grouped as the conversion (CONV) group and completed laparoscopic (LAP) group. The 1:2 propensity score matching was performed to reduce the effect of bias due to the imbalanced baseline features between the two groups. Multivariate analyses were performed to identify risk factors for conversion and poor survival.

Results: After propensity-score matching, 168 patients (56 in the CONV group and 112 in the LAP group) were studied. The CONV group was associated with significantly longer operation time (252.4 vs. 216.7 min, P < 0.001) and greater estimated blood loss (234.8 vs. 171.2 ml, P < 0.001) as compared with the LAP group. The time to first flatus (3.8 vs. 3.3 days, P = 0.043), time to start a liquid diet (4.1 vs. 3.5 days, P = 0.021), and postoperative hospital stay (8.7 vs. 7.6 days, P = 0.020) were significantly longer in the CONV group than that in the LAP group. The overall complication rate did not differ significantly between the CONV group and the LAP group (16.1% vs. 12.5%, P = 0.692). Both 5-year overall survival (OS) and 5-year disease-free survival (DFS) did not differ significantly between the CONV group and the LAP group (P = 0.805, P = 0.945, respectively). Multivariate analysis showed that lymphovascular invasion and stage III were independent prognostic factors for poor OS and DFS, whereas conversion was not.

Conclusions: The conversion from laparoscopic to open gastrectomy had no negative impact on morbidity and long-term survival outcomes for patients with locally AGC.
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http://dx.doi.org/10.1007/s11605-021-04975-6DOI Listing
April 2021

A Novel Finger Vein Recognition Method Based on Aggregation of Radon-Like Features.

Sensors (Basel) 2021 Mar 8;21(5). Epub 2021 Mar 8.

Artificial Intelligence and Computer Vision Laboratory, University of Electronic Science and Technology of China, Zhongshan Institute, Zhongshan 528402, China.

Finger vein (FV) biometrics is one of the most promising individual recognition traits, which has the capabilities of uniqueness, anti-forgery, and bio-assay, etc. However, due to the restricts of imaging environments, the acquired FV images are easily degraded to low-contrast, blur, as well as serious noise disturbance. Therefore, how to extract more efficient and robust features from these low-quality FV images, remains to be addressed. In this paper, a novel feature extraction method of FV images is presented, which combines curvature and radon-like features (RLF). First, an enhanced vein pattern image is obtained by calculating the mean curvature of each pixel in the original FV image. Then, a specific implementation of RLF is developed and performed on the previously obtained vein pattern image, which can effectively aggregate the dispersed spatial information around the vein structures, thus highlight vein patterns and suppress spurious non-boundary responses and noises. Finally, a smoother vein structure image is obtained for subsequent matching and verification. Compared with the existing curvature-based recognition methods, the proposed method can not only preserve the inherent vein patterns, but also eliminate most of the pseudo vein information, so as to restore more smoothing and genuine vein structure information. In order to assess the performance of our proposed RLF-based method, we conducted comprehensive experiments on three public FV databases and a self-built FV database (which contains 37,080 samples that derived from 1030 individuals). The experimental results denoted that RLF-based feature extraction method can obtain more complete and continuous vein patterns, as well as better recognition accuracy.
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http://dx.doi.org/10.3390/s21051885DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7962657PMC
March 2021

Alkaline phosphatase-regulated in situ formation of chromogenic probes for multicolor visual sensing of biomarkers.

Talanta 2021 Jun 19;228:122222. Epub 2021 Feb 19.

Research Center for Analytical Sciences, Northeastern University, Shenyang, 110819, PR China. Electronic address:

Alkaline phosphatase (ALP), as an immunological label, is widely used in biochemical assays. Here, a simple yet effective strategy for ALP activity detection was proposed on the basis of in situ formation of Prussian blue nanoparticles and polychromatic superposition effect. Firstly, ascorbic acid, a product from ALP-catalyzed hydrolysis of 2-phospho-l-ascorbic acid (AAP), converted yellow ferricyanide into ferrocyanide. Then, the specific reaction between ferrocyanide and ferric ions (Fe) initiated the generation of Prussian blue nanoparticles in situ. Meanwhile, the residual AAP chelated with Fe, and a stable Fe-AAP complex was quickly formed. When Prussian blue nanoparticles mixed with brown Fe-AAP complex and yellow ferricyanide at different ratios, a distinct color variation was presented. Therefore, a sensitive multicolor assay of ALP activity with a detection limit of 1.0 U/L was realized by simply blending commercially available reagents. Furthermore, magnetic sandwich and competitive sensing platforms for multiple biomarkers detection were constructed by combining the ALP-regulated multicolor system with the well-developed aptasensor. The feasibility of the sensors was convincingly demonstrated by using thrombin and prostate specific antigen as model targets. In addition, the proposed multicolor strategy was employed for evaluating inhibition efficiency, and shows potential in visual screening of enzyme inhibitors. Such a facile, sensitive and low-cost sensing strategy provides a new perspective to develop universal platforms of point-of-care testing.
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http://dx.doi.org/10.1016/j.talanta.2021.122222DOI Listing
June 2021

Simple, rapid, and sensitive on-site detection of Hg in water samples through combining portable evanescent wave optofluidic biosensor and fluorescence resonance energy transfer principle.

Anal Chim Acta 2021 Apr 25;1155:338351. Epub 2021 Feb 25.

State Key Laboratory of NBC Protection for Civilian, Beijing, 102205, China. Electronic address:

Rapid and sensitive detection of Hg in the environment and drinking water is vital because of its non-degradability, bioaccumulation, and high toxicity. Herein, we report a portable evanescent wave optofluidic biosensor (EWOB) for simple sensitive detection of Hg using fluorescence labeled poly-A DNA strand (CY-A14) and quencher labeled poly-T DNA strand (BQ-T14) as signal reporter and biorecognition element, respectively. Both CY-A14 and Hg can competitively bind with BQ-T14 based on DNA hybridization and the specifical binding of Hg and T bases of DNA to form T-Hg-T mismatch structure, respectively. Higher concentration of Hg lead to less CY-A14 bound to BQ-T14 and thus a higher fluorescence intensity. The influence of several key environmental factors on Hg biosensor, such as pH, temperature, and ionic strength, was investigated in details because they were essential for practical applications of Hg biosensor. Under optimal conditions, a detection cycle for a single sample, including the measurement and regeneration, was less than 10 min with a Hg detection limit of 8.5 nM. The high selectivity of the biosensor was showed by evaluating its response to various potentially interfering metal ions. Our results clearly demonstrated that the portable EWOB could serve as a powerful tool for rapid and sensitive on-site detection of Hg in real water samples. The EWOB is also potentially applicable to detect other heavy metal ions or small molecule targets for which DNA/aptamers could be applied as specific biosensing probes.
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http://dx.doi.org/10.1016/j.aca.2021.338351DOI Listing
April 2021

Gene expression profile of the taimen Hucho taimen in response to acute temperature changes.

Comp Biochem Physiol Part D Genomics Proteomics 2021 06 15;38:100824. Epub 2021 Mar 15.

Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China; Key Laboratory of Aquatic Animal Diseases and Immune Technology of Heilongjiang Province, Harbin 150070, China. Electronic address:

The endangered cold-water fish species taimen (Hucho taimen) suffer acute temperature changes in culture and wild conditions. Understanding the effects of acute temperature changes on physiological processes of this species is essential for aquaculture practices and conservation. Liver transcriptomic profiles of the taimen (n = 24) exposed to acute temperature decrease (from 20 °C to 10 °C) and acute temperature increase (from 10 °C to 20 °C) was evaluated using high-throughput RNA-Sequencing. Samples were collected at day 0, 1, 7 and 35 in both treatments. Compared to day 0, the total numbers of differentially expressed genes (DEGs) in the taimen after acute temperature decrease were 173, 226 and 42 at day 1, 7 and 35, respectively, and the total numbers of DEGs following acute temperature increase were 260, 253 and 282 at day 1, 7 and 35, respectively. Particularly, 14 key regulatory genes were commonly found between both acute temperature treatments. Functional analysis based on the commonly identified DEGs revealed important metabolic pathways related to metabolism and immune function, suggesting a specific response mechanism of taimen against cold and heat shock. The results may assist in developing management strategies for stress mediation caused by acute temperature changes in the taimen and other cold water fish.
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http://dx.doi.org/10.1016/j.cbd.2021.100824DOI Listing
June 2021

Resveratrol protects MC3T3-E1 cells against cadmium-induced suppression of osteogenic differentiation by modulating the ERK1/2 and JNK pathways.

Ecotoxicol Environ Saf 2021 May 5;214:112080. Epub 2021 Mar 5.

Department of Chinese Medicine, The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong 510630, PR China; School of Traditional Chinese Medicine, Jinan University, Guangzhou, Guangdong 510630, PR China. Electronic address:

Resveratrol (RES) is a natural polyphenolic compound with a broad range of physiological and pharmacological properties. Previous studies have shown that RES also plays an important role in protecting and promoting early bone metabolism and differentiation. The accumulation of cadmium (Cd), one of the world's most poisonous substances, can inhibit skeletal growth and bone maturation, thus causing osteoporosis. However, whether RES can prevent the Cd-induced inhibition of osteogenic differentiation remains unknown. In this study, we found that RES promoted the early maturity of osteoblastic MC3T3-E1 cells, as demonstrated by the significantly increased mRNA and protein expression of a range of differentiation markers, including alkaline phosphatase (ALP), collagen 1 (COL1), bone morphogenetic protein-2 (BMP-2), and runt-related transcription factor 2 (RUNX2). In contrast, we found that cadmium chloride (CdCl) inhibited the viability and osteogenic maturity of MC3T3-E1 cells. We also demonstrated that RES pretreatment for 30 min provided significant protection against Cd-induced apoptosis and attenuated the inhibition of osteogenic differentiation induced by Cd by modulating ERK1/2 and JNK signaling. In conclusion, our results indicate that RES is a potential femoral protectant that not only enhance the viability and early differentiation of osteoblasts, but also protect osteoblasts from cadmium damage.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112080DOI Listing
May 2021

Polidocanol Sclerotherapy for the Treatment of Pyogenic Granuloma in Children.

Dermatol Surg 2021 Jun;47(6):802-804

All authors are affiliated with the Department of Vascular Anomalies and Interventional Radiology, Qilu Children's Hospital of Shandong University, Jinan City, Shandong, China.

Background: Pyogenic granuloma (PG) is a benign vascular lesion that is commonly observed in the skin and mucosa. Sclerotherapy is the first-line conservative treatment option for PG.

Objective: This retrospective study aimed to evaluate the efficacy of sclerotherapy with 1.0% polidocanol for the treatment of PG.

Materials And Methods: All patients who were clinically diagnosed with PG consecutively at the Pediatric Outpatient Service of Qilu Children's Hospital of Shandong University from March 2018 to October 2019 received sclerotherapy with 1.0% polidocanol.

Result: The procedure resulted in the complete excision of PG, with inconspicuous scars. The procedure was well-tolerated, and recurrence was not observed. Allergic reactions, cutaneous necrosis, and pigmentary changes were not observed.

Conclusion: Sclerotherapy with 1.0% polidocanol is considered an effective treatment for PGs in children. Early treatment was associated with a more favorable outcome.
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http://dx.doi.org/10.1097/DSS.0000000000002967DOI Listing
June 2021

Transcriptome analysis of Cyclocarya paliurus flavonoids regulation of differently expressed genes in Enterococcus faecalis under low pH stress.

Arch Microbiol 2021 Jul 21;203(5):2147-2155. Epub 2021 Feb 21.

Department of Food Science and Engineering, Ningbo University, Ningbo, 315211, People's Republic of China.

Enterococcus faecalis (E. faecalis) is an indigenous intestinal bacterium and has potential to be applied as probiotic supplement. Low pH is one of the main stresses that E. faecalis has to deal with to colonize in the gastrointestinal tract. Previous study indicated low concentration of flavonoids may enhance the tolerance of probiotic to environmental stress. In the present research, transcriptome analysis was employed to investigate the influence of Cyclocarya paliurus flavonoids (CPF) on E. faecalis exposed to low pH environment. The results revealed that under the stress of low pH, genes related to cell wall and membrane, transmembrane transport, metabolism process, energy production, and conversion stress proteins were significantly differentially expressed. And certain undesired changes of which (such as genes for MFS transporter were downregulated) could be partially mitigated by CPF intervention, indicating their capacity to improve the low pH tolerance of E. faecalis. Results from this study deepened our understanding of the beneficial role of CPF on the probiotic in the gastrointestinal environment.
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http://dx.doi.org/10.1007/s00203-021-02215-8DOI Listing
July 2021

Visible-Light-Driven, Photocatalyst-Free Cascade to Access 3-Cyanoalkyl Coumarins from -Hydroxycinnamic Esters.

J Org Chem 2021 Mar 19;86(5):4245-4253. Epub 2021 Feb 19.

College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, P. R. China.

A visible-light-driven, photocatalyst-free route starting from easily accessed -hydroxycinnamic esters and -perfluoropyridin-4-yl oximes has been successfully developed to rapidly assemble a wide range of 3-cyanoalkyl coumarins. This process does not require addition of external photocatalysts, exhibiting beneficial features including mild reaction conditions, synthetic simplicity, and excellent substrate compatibility. Extensive mechanistic investigations revealed that the in situ generated phenolate anions served as photosensitizers to drive this photoinduced transformation.
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http://dx.doi.org/10.1021/acs.joc.1c00024DOI Listing
March 2021
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