Publications by authors named "Dan Peng"

173 Publications

IgG4-Related Disease: A Retrospective Chinese Study of Features and Treatment Response of 98 Patients Including 4 Rare Cases.

Curr Med Sci 2021 Apr 20;41(2):390-397. Epub 2021 Apr 20.

Department of Radiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.

The features and treatment of 98 Chinese patients with immunoglobulin G4 (IgG4)-related disease (IgG4-RD) referred to a single tertiary referring centre were reviewed. Patients diagnosed with IgG4-RD according to the comprehensive diagnostic criteria (CDC) were included in the retrospective study from May 2012 to March 2019. We collected data on clinical, laboratory, imaging, histological features and treatment. Totally, 98 patients with IgG4-RD were enrolled. The common clinical manifestations included abdominal pain, salivary gland swelling and lymphadenopathy. 51% of the patients had multiple organs involvement. Lymph nodes, pancreas and salivary glands were most commonly involved. Four rare sites including ulna, cerebellum, scalp, and mammary gland were found. The serum IgG4 level was increased by 85.7%. The serum IgG4 level was positively correlated with the number of involved organs, IgG and IgG4/IgG. Low C3 and C4 levels were observed in 37.5% and 12.2% patients respectively, and all patients with kidney involvement had hypocomplementemia. A total of 54 patients underwent tissue biopsies, and 55.6%, 31.5% and 11.1% cases were diagnosed as definite, probable and possible IgG4-RD, respectively. Eighty-eight patients received glucocorticoids (GCs) therapy. Five patients underwent radical surgery to remove the lesion. 73% of them presented a complete or partial remission. IgG4-RD is a systemic fibroinflammatory disease with involvement of multiple organs throughout the body including some rare sites. Most IgG4-RD patients had increased serum IgG4 levels and patients with kidney involvement showed hypocomplementemia. GCs therapy is effective. More research is needed to provide a more reliable basis for the diagnosis and treatment of patients.
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http://dx.doi.org/10.1007/s11596-021-2359-5DOI Listing
April 2021

Mechanism insight into the role of clay particles on enhancing phosphate removal by ferrate compared with ferric salt.

Environ Sci Pollut Res Int 2021 Apr 17. Epub 2021 Apr 17.

Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China.

The application of ferrate (Fe(VI)) and ferric chloride as coagulants for treating phosphate wastewater in the presence of kaolin clay particles was comparatively studied. The phosphate removal processes by ferrate and ferric chloride assisted with kaolin clay particles were investigated under different Fe/P molar ratios. At neutral pH, complete removal of phosphates by ferrate and ferric chloride was observed at 2:1 and 6:1 of Fe/P molar ratio, respectively. The effect of kaolin clay particles on the phosphate removal process was discussed by zeta potential, size particle distribution, FTIR and XPS. We showed that with the increase of Fe/P molar ratio, the interaction intensity of kaolin clay particles with Fe flocs was decreased by ferric chloride coagulation while firstly increased and then decreased by ferrate. This depends on the Fe species with positive charge from ferric chloride hydrolysis and ferrate decomposition. Phosphate can inhibit the formation of FeOH and Fe(OH) in the ferric chloride hydrolysis but promote the formation of FeOOH and Fe(OH) in the ferrate decomposition. Kaolin clay particles can more remarkably promote phosphate removal by ferrate than by ferric chloride.
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http://dx.doi.org/10.1007/s11356-021-13436-1DOI Listing
April 2021

Microarray Expression Profile and Analysis of Circular RNA Regulatory Network in Pulmonary Thromboembolism.

Int J Gen Med 2021 9;14:1239-1249. Epub 2021 Apr 9.

Department of Respiratory and Critical Care Medicine, Beijing Luhe Hospital, Capital Medical University, Beijing, People's Republic of China.

Background: Pulmonary thromboembolism (PTE) is a common disease which may be a serious condition and has high mortality. Recently, it has been shown that circRNAs play an important role in the development of various diseases, including thromboembolic disease. However, circRNAs expression profiling is not clear in PTE, this study aims to identify the circRNAs expressed in PTE and to elucidate their possible role in pathophysiology of PTE.

Methods: A total of 5 patients with CTPA-confirmed PTE and 5 healthy controls were recruited for the present study. The circRNAs expression profile was analyzed by microarray.

Results: In total, 256 differentially expressed circRNAs (up 142, down114) and 1162 mRNA (up 446, down 716) were summarized by analyzing the circRNAs microarray data. The top 3 up-regulated and 3 down-regulated circRNAs were validated by Real-Time Polymerase Chain Reaction (qRT-PCR). Two differentially expressed circRNAs (hsa_circ_0000891, hsa_circ_0043506) were selected for further analysis. Finally, we construct a circRNA-miRNA-mRNA ceRNA network with a bioinformatic prediction tool. Pathway analysis shows that the enriched mRNAs targets take part in Protein processing in endoplasmic reticulum, Systemic lupus erythematosus, Endocytosis, Spliceosome, HTLV-I infection and Ubiquitin mediated proteolysis.

Conclusion: Our findings indicated that aberrantly expressed circRNAs (hsa_circ_0000891, hsa_circ_0043506) may be involved in the development of PTE.
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http://dx.doi.org/10.2147/IJGM.S304199DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8043787PMC
April 2021

Multiple adsorption systems and electron-scale insights into the high efficiency coadsorption of a novel assembled cellulose via experiments and DFT calculations.

J Hazard Mater 2021 Mar 27;416:125748. Epub 2021 Mar 27.

College of Environment, Jinan University, Guangzhou 510632, PR China.

In view of the characteristics of heavy metal and antibiotic compound pollution in the Pearl River Basin in Guangzhou. More scientifically modified cellulose, named HVUC, is characterized by multiple hydrophilic groups, long chains and large space and displays highly efficient adsorption of both Cd and sulfamethoxazole (SMZ) and good adaptability in a wide pH range and at high ion strength. Furthermore, the coadsorption mechanism was elaborated from multiple angles. Multiple adsorption experiments explained the competition and synergy effect in the adsorption process. The electrostatic potential maps indicated that HVUC had advantageous adsorption sites for both Cd and SMZ and that electrostatic interactions had the greatest impact on the adsorption of Cd and SMZ. The electron density and differential charge density images proved that Cd more easily overlapped electron clouds and transferred electrons with HVUC and that SMZ and could act as a bridge for SMZ. The equilibrium configuration indicated that the formation of Cd-SMZ complexes led to the bending and folding of SMZ, which was not conducive to overall adsorption when SMZ was close to HVUC and could lead to the release of SMZ when Cd was close to HVUC, which confirmed the proposed mechanism of complexation-decomplexation-complexation.
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http://dx.doi.org/10.1016/j.jhazmat.2021.125748DOI Listing
March 2021

Genome sequencing and comparative genome analysis of 6 hypervirulent Klebsiella pneumoniae strains isolated in China.

Arch Microbiol 2021 Apr 3. Epub 2021 Apr 3.

School of Public Health and Management, Chongqing Medical University, NO.1 Yixueyuan Road, Yuzhong District, Chongqing, 400016, China.

Hypervirulent Klebsiella pneumoniae (hvKP) has been increasingly reported over the past three decades and causes severe infections. To increase our understanding of hvKP at the genome level, genome sequencing and comparative genome analysis were performed on 6 hvKPs. The whole genome DNA from 6 hvKPs with different capsular serotypes isolated in China was extracted. The genome sequencing and assembly results showed the genome size of the six hvKPs and GC content. Comparative analyses of the genomes revealed the gene homology and genome rearrangement in the 6 hvKPs compared with Klebsiella pneumonia NTUH-K2044. The phylogenetic tree based on full-genome SNPs of the 7 hvKPs showed that NTUH-K2044 formed a single clade, showing distant evolutionary distances with the other six strains, and the non-K1 hvKP strains had a relatively closer phylogenetic relationship. BLAST comparison analysis found that some selected virulence genes had different degrees of deletion in the non-K1 hvKPs. SNP-based virulence gene mutation analysis showed that some virulence genes had different degrees of SNP mutations. The whole-genome sequencing and comparative genome analysis of six hvKP strains with NTUH-K2044 provide us with a basic understanding of the genome composition, genetic polymorphism, evolution and virulence genes of hvKP and a basis for further research on these genes and the pathogenesis of hvKP.
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http://dx.doi.org/10.1007/s00203-021-02263-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8019302PMC
April 2021

Noninvasive Prenatal Paternity Testing with a Combination of Well-Established SNP and STR Markers Using Massively Parallel Sequencing.

Genes (Basel) 2021 Mar 22;12(3). Epub 2021 Mar 22.

Faculty of Forensic Medicine, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou 510080, China.

Cell-free fetal DNA (cffDNA) from maternal plasma has made it possible to develop noninvasive prenatal paternity testing (NIPPT). However, most studies have focused on customized single nucleotide polymorphism (SNP) typing systems and few have used conventional short tandem repeat (STR) markers. Based on massively parallel sequencing (MPS), this study used a widely-accepted forensic multiplex assay system to evaluate the effect of noninvasive prenatal paternity testing with a combination of well-established SNP and STR markers. Using a ForenSeq DNA Signature Prep Kit, NIPPT was performed in 17 real parentage cases with monovular unborn fetuses at 7 to 24 gestational weeks. Different analytical strategies for the identification of paternally inherited allele (PIA) were developed to deal with SNPs and STRs. Combined paternity index (CPI) for 17 real trios as well as 272 unrelated trios was calculated. With the combination of SNPs and A-STRs, 82.35% (14/17), 88.24% (15/17), 94.12% (16/17), and 94.12% (16/17) of real trios could be accurately determined when the likelihood ratio (LR) threshold for paternity inclusion was set to 10,000, 1000, 100, and 10, respectively. This reveals that simultaneous surveys of SNP and STR markers included in the ForenSeq DNA Signature Prep Kit offer a promising method for NIPPT using MPS technology.
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http://dx.doi.org/10.3390/genes12030454DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8004970PMC
March 2021

Clinical Effect of Traditional Chinese Medicine Shenhuang Granule in Critically Ill Patients with COVID-19: A Single-Centered, Retrospective, Observational Study.

J Microbiol Biotechnol 2021 Mar;31(3):380-386

Department of Critical Care Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, P.R. China.

The coronavirus disease 2019 (COVID-19) pandemic has become a public health emergency of global concern. In China, traditional Chinese medicine has been widely administered to COVID-19 patients without sufficient evidence. To evaluate the efficacy of Shenhuang Granule (SHG) for treating critically ill patients with COVID-19, we included in this study 118 patients who were admitted to the ICU of Tongji Hospital between January 28, 2020 and March 28, 2020. Among these patients, 33 (27.9%) received standard care plus SHG (treatment group) and 85 (72.1%) received standard care alone (control group). Enrolled patients had a median (IQR) age of 68 (57-75) years, and most (79 [67.1%]) were men. At end point of this study, 83 (70.3%) had died in ICU, 29 (24.5%) had been discharged from ICU, and 6 patients (5.2%) were still in ICU. Compared with control group, mortality was significantly lower in treatment group (45.4% vs. 80%, < .001). Patients in treatment group were less likely to develop acute respiratory distress syndrome (ARDS) (12 [36.3%] vs. 54 [63.5%], = 0.012) and cardiac injury (5 [15.1%] vs. 32 [37.6%], = 0.026), and less likely to receive mechanical ventilation (22 [66.7%] vs. 72 [84.7%], = 0.028) than those in control group. The median time from ICU admission to discharge was shorter in treatment group (32 [20-73] days vs. 76 [63-79] days, = 0.0074). These findings suggest that SHG treatment as a complementary therapy might be effective for critically ill adults with COVID-19 and warrant further clinical trials.
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http://dx.doi.org/10.4014/jmb.2009.09029DOI Listing
March 2021

Increasing inundation frequencies enhance the stochastic process and network complexity of soil archaeal community in coastal wetlands.

Appl Environ Microbiol 2021 Mar 19. Epub 2021 Mar 19.

State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, 71 East Beijing Road, Nanjing 210008, China

Coastal wetlands are experiencing frequent flooding because of global climate changes, such as the rising sea level. Despite the key role of archaea in soil biogeochemical cycles, the assembly processes and co-occurrence patterns of archaeal communities in coastal wetlands in response to increasing inundation frequencies remain elusive. Herein, we established an mesocosm with an inundation frequency gradient to investigate the response of soil archaeal community toward increasing inundation frequencies in monocultures of (SA) and a mangrove species, (KO). Both neutral community model and null model analyses suggested that stochastic processes are dominant in governing the archaeal community assembly, and that the stochastic processes are enhanced with increasing inundation frequencies. Increasing inundation frequencies significantly increased the community niche width. Moreover, archaeal community in SA soil displayed lower niche overlap and higher stochasticity than in KO soil. Co-occurrence network analysis revealed that the network complexity increases with increase in the inundation frequencies. Soil water content is the most decisive factor influencing the archaeal communities. Overall, we found that increasing inundation frequencies enhance the stochastic processes and network complexity of the soil archaeal community in coastal wetlands. This study could enhance our understanding on the response of soil archaeal communities in coastal wetlands toward global change.Coastal wetlands, subjected to regular disturbances by periodic tidal, are highly productive and important in the regulation of climate change. However, the assembly mechanisms and co-occurrence patterns of soil archaeal communities in coastal areas remain poorly known, especially for their responses to increasing inundation frequencies. In this study, we aim at unravelling these uncertainties by studying typical estuarine ecosystems in southern China. We show that increasing inundation frequencies enhance the stochastic processes and network complexity of the soil archaeal community. This study offers a new path for an improved understanding of archaeal community assembly and species coexistence in coastal environments, with a special focus on the role of inundation frequency.
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http://dx.doi.org/10.1128/AEM.02560-20DOI Listing
March 2021

Multiscale Dynamics of Blood Pressure Fluctuation Is Associated With White Matter Lesion Burden in Older Adults With and Without Hypertension: Observations From a Pilot Study.

Front Cardiovasc Med 2021 26;8:636702. Epub 2021 Feb 26.

Hinda and Arthur Marcus Institute for Aging Research, Hebrew SeniorLife, Boston, MA, United States.

White matter lesions (WMLs) are highly prevalent in older adults, and hypertension is one of the main contributors to WMLs. The blood pressure (BP) is regulated by complex underlying mechanisms over multiple time scales, thus the continuous beat-to-beat BP fluctuation is complex. The association between WMLs and hypertension may be manifested as diminished complexity of BP fluctuations. The aim of this pilot study is to explore the relationships between hypertension, BP complexity, and WMLs in older adults. Fifty-three older adults with clinically diagnosed hypertension and 47 age-matched older adults without hypertension completed one MRI scan and one BP recording of 10-15 min when sitting quietly. Their cerebral WMLs were assessed by two neurologists using the Fazekas scale based on brain structural MRI of each of their own. Greater score reflected higher WML grade. The complexity of continuous systolic (SBP) and diastolic (DBP) BP series was quantified using multiscale entropy (MSE). Lower MSE reflected lower complexity. Compared to the non-hypertensive group, hypertensives had significantly greater Fazekas scores ( > 5.3, < 0.02) and lower SBP and DBP complexity ( > 8.6, < 0.004). Both within each group (β < -0.42, < 0.01) and across groups (β < -0.47, < 0.003), those with lower BP complexity had higher Fazekas score. Moreover, complexity of both SBP and DBP mediated the influence of hypertension on WMLs (indirect effects > 0.25, 95% confidence intervals = 0.06 - 0.50). These results suggest that diminished BP complexity is associated with WMLs and may mediate the influence of hypertension on WMLs. Future longitudinal studies are needed to examine the causal relationship between BP complexity and WMLs.
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http://dx.doi.org/10.3389/fcvm.2021.636702DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7952298PMC
February 2021

Advances in the role of auxin for transcriptional regulation of lignin biosynthesis.

Funct Plant Biol 2021 Mar 5. Epub 2021 Mar 5.

Lignin is a natural polymer interlaced with cellulose and hemicellulose in secondary cell walls (SCWs). Auxin acts via its signalling transduction to regulate most of plant physiological processes. Lignification responds to auxin signals likewise and affects the development of anther and secondary xylem in plants. In this review, the research advances of AUXIN RESPONSE FACTOR (ARF)-dependent signalling pathways regulating lignin formation are discussed in detail. In an effort to facilitate the understanding of several key regulators in this process, we present a regulatory framework that comprises protein-protein interactions at the top and protein-gene regulation divided into five tiers. This characterises the regulatory roles of auxin in lignin biosynthesis and links auxin signalling transduction to transcriptional cascade of lignin biosynthesis. Our works further point to several of significant problems that need to be resolved in the future to gain a better understanding of the underlying mechanisms through which auxin regulates lignin biosynthesis.
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http://dx.doi.org/10.1071/FP20381DOI Listing
March 2021

Crm1-Dependent Nuclear Export of Bach1 is Involved in the Protective Effect of Hyperoside on Oxidative Damage in Hepatocytes and CCl-induced Acute Liver Injury.

J Inflamm Res 2021 25;14:551-565. Epub 2021 Feb 25.

Department of Pharmacy, Daping Hospital, Army Medical University, Chongqing, 400042, People's Republic of China.

Background: Nrf2-Bach1 antioxidant signaling pathway is considered as one of the most important mechanisms of cellular resistance to oxidative injury. The effect of hyperoside (Hyp) on the expression and distribution of Bach1, the relationship of Hyp's antioxidative effect and the influence of Bach1 remains unclear.

Purpose: The aim of this study was to investigate the role and mechanisms of Bach1 in the protective effect of Hyp on oxidative liver injury.

Methods: The protective effect of Hyp on oxidative stress injury was observed in vivo and in vitro. Next, the influence of Hyp on Bach1 expression and distribution, and competitive combination of Nrf2-Bach1 with ARE in HO-induced L02 cell was studied by Western blot, RT-PCR, immunofluorescence and CHIP assay. Finally, the expressions of Crm1, ERK and p38 and their roles on Hyp mediated nuclear export of Bach1 were investigated by Western blot.

Results: Hyp ameliorated the pathological damage, reduced the liver index, AST, ALT and MDA activities, and increased SOD and GSH levels in the CCl-induced acute liver injury mouse model. Hyp attenuated HO-induced oxidative stress injury in L02 cells. Hyp promoted the early rapid redistribution of Bach1 from nucleus to cytoplasm. CHIP analyses demonstrated that Hyp enhanced the levels of Nrf2-ARE complex, and weakened the levels of Bach1-ARE complex within three hours. In addition, Hyp enhanced transport protein Crm1 expression and ERK1/2 activity. And LMB, a Crm1 inhibitor, attenuated the effect of Hyp on Bach1 nuclear export and anti-oxidation. U0126, an ERK1/2 inhibitor, reduced the effect of Hyp on Crm1 expression and the Bach1 redistribution.

Conclusion: The hepatoprotective mechanism of Hyp was related to improve Bach1 nuclear export depending on ERK1/2-Crm1 to upregulate the level of Nrf2 binding to ARE.
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http://dx.doi.org/10.2147/JIR.S279249DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7920627PMC
February 2021

Effective multi-functional biosorbent derived from corn stalk pith for dyes and oils removal.

Chemosphere 2021 Jun 12;272:129963. Epub 2021 Feb 12.

College of Natural Resources and Environment, Northwest A&F University, Yangling, 712100, China.

In this study, malic acid-modified corn stalk pith (MA-CSP) was prepared as an environmentally friendly multi-functional bio-sorbent for adsorbing of dyes and oils. The sorption capacity of the MA-CSP for single and binary dyes is 328.46 mg/g - 566.27 mg/g. In addition, the MA-CSP also had good sorption for lubricating oil, soybean oil, diesel oil, and isopropyl alcohol, which were 37.2 g/g, 44.1 g/g, 33.8 g/g, and 29.3 g/g, respectively. Physical and statistical models were used to analyze the adsorption behavior of methylene blue (MB) and crystal violet (CV). And its sorption behavior for dyes was also affected by the co-existing salts in water. The sorption mechanism of the dye was mainly electrostatic attraction and hydrogen bonding action. The sorption of oil was primarily via the role of van der Waals force and hydrophobic interaction. The MA-CSP, as an eco-friendly, economical and efficient multi-functional sorbent, holds promise for effective dyes and oil removal from contaminated water, and its application in other fields is also highly anticipated.
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http://dx.doi.org/10.1016/j.chemosphere.2021.129963DOI Listing
June 2021

Characteristics and removal efficiency of microplastics in sewage treatment plant of Xi'an City, northwest China.

Sci Total Environ 2021 Jun 23;771:145377. Epub 2021 Jan 23.

College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi 712100, China; Key Laboratory of Plant Nutrition and the Agro-environment in Northwest China, Ministry of Agriculture, Yangling, Shaanxi 712100, China. Electronic address:

Wastewater treatment plants (WWTP) are the important source of microplastics (MPs) in the environment, but the distribution and property of MPs in WWTPs are not fully understood. The study systematically investigated the transportation, characteristics and fate of MPs in both sewage and sludge of WWTP in Xi'an, a western region of China to complement the research on the fate of MPs in WWTP in inland China. The consequence was abundance, size, shape, color and type of MPs in sewage and sludge of different treatment processes were analyzed and compared by the metallurgical microscope and Fourier Transform Infrared (FTIR) spectrometer. Results showed that the MP abundance decreased from 288.5 n/L of the influent to 22.9 n/L of the effluent, corresponding to the removal rate of 92.1%. Among them, the secondary treatment has the best removal effect, with a removal rate of 72.1%. With the treatment process, the distribution of MPs with different characteristics is constantly changing, which leads to differences in the distribution of MPs in sludge and sewage. It is speculated from the characteristics of MPs that domestic sewage is still the main source of microplastics in Beishiqiao WWTP. Restricting the use of plastic products in daily life or finding alternatives is of great significance to the construction of MPs pollution.
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http://dx.doi.org/10.1016/j.scitotenv.2021.145377DOI Listing
June 2021

Developmental validation of the MGIEasy Signature Identification Library Prep Kit, an all-in-one multiplex system for forensic applications.

Int J Legal Med 2021 May 1;135(3):739-753. Epub 2021 Feb 1.

Faculty of Forensic Medicine, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, People's Republic of China.

Analyzing genetic markers in nuclear and mitochondrial genomes is helpful in various forensic applications, such as individual identifications and kinship analyses. However, most commercial kits detect these markers separately, which is time-consuming, laborious, and more error-prone (mislabelling, contamination, ...). The MGIEasy Signature Identification Library Prep Kit (hereinafter "MGIEasy identification system"; MGI Tech, Shenzhen, China) has been designed to provide a simple, fast, and robust way to detect appropriate markers in one multiplex PCR reaction: 52 autosomal STRs, 27 X-chromosomal STRs, 48 Y-chromosomal STRs, 145 identity-informative SNPs, 53 ancestry-informative SNPs, 29 phenotype-informative SNPs, and the hypervariable regions of mitochondrial DNA (mtDNA). Here, we validated the performance of MGIEasy identification system following the guidelines of the Scientific Working Group on DNA Analysis Methods (SWGDAM), assessing species specificity, sensitivity, mixture identification, stability under non-optimal conditions (degraded samples, inhibitor contamination, and various substrates), repeatability, and concordance. Libraries prepared using MGIEasy identification system were sequenced on a MGISEQ-2000 instrument (MGI Tech). MGIEasy-derived STR, SNP, and mtDNA genotypes were highly concordant with CE-based STR genotypes (99.79%), MiSeq FGx-based SNP genotypes (99.78%), and Sanger-based mtDNA genotypes (100%), respectively. This system was strongly human-specific, resistant to four common PCR inhibitors, and reliably amplified both low quantities of DNA (as low as 0.125 ng) and degraded DNA (~ 150 nt). Most of the unique alleles from the minor contributor were detected in 1:10 male-female and male-male mixtures; some minor Y-STR alleles were even detected in 1:1000 male-female mixtures. MGIEasy also successfully directly amplified markers from blood stains on FTA cards, filter papers, and swabs. Thus, our results demonstrated that MGIEasy identification system was suitable for use in forensic analyses due to its robust and reliable performance on samples of varying quality and quantity.
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http://dx.doi.org/10.1007/s00414-021-02507-0DOI Listing
May 2021

Identification and sequencing of 59 highly polymorphic microhaplotypes for analysis of DNA mixtures.

Int J Legal Med 2021 Jan 27. Epub 2021 Jan 27.

Faculty of Forensic Medicine, Zhongshan School of Medicine, Sun Yat-sen University, No. 74 Zhongshan Road II, Guangzhou, 510080, Guangdong, People's Republic of China.

Mixture detection remains one of the major challenges within a forensic science context. In recent years, microhaplotypes were proposed to have great potential in mixture detection, although many of them are not as polymorphic as widely used short tandem repeat (STR) markers. In this study, 59 new highly polymorphic microhaplotypes were identified and sequenced with the NextSeq 500 Sequencer. Based on the whole 1000 Genomes Project dataset, the average effective number of alleles (Ae) of the 59 microhaplotypes was 5.44, and the Ae values of 36 of these microhaplotypes were > 5.00. Their genetic variations in 187 Han Chinese individuals were evaluated. The average allele coverage ratio (ACR) of heterozygotes across all loci was 0.96 ± 0.05. The number of observed alleles varied from 4 to 23, with an average of 8.8 alleles per microhaplotype locus. The average observed heterozygosity (Ho) of 59 loci was 0.77 ± 0.05, and the Ho values of 15 of these loci were > 0.80. All loci showed high polymorphisms with a discrimination power (DP) ranging from 0.80 to 0.97, and the average DP was 0.92 ± 0.03. The analysis of simulated mixtures demonstrated that the microhaplotypes reported here were highly polymorphic and performed well in forensic DNA mixture analysis. This study not only demonstrated the applicability of microhaplotypes in mixture analysis but also provided new choices for highly polymorphic microhaplotypes because after adding the markers identified here, the number of microhaplotypes with Ae values of > 4.00 will increase from ~ 50 to ~ 110.
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http://dx.doi.org/10.1007/s00414-020-02483-xDOI Listing
January 2021

Iron Sucrose: A Double-Edged Sword in High Phosphate Media-Induced Vascular Calcification.

Calcif Tissue Int 2021 Jan 13. Epub 2021 Jan 13.

Neonatology Department, The First People's Hospital of Jingmen, Jingmen, 448000, Hubei, People's Republic of China.

The high incidence of vascular calcification (VC) in patients with chronic kidney disease (CKD) has become an important clinical subject. Hyperphosphatemia is a primary cause of CKD-related VC. Intravenous iron sucrose (IS) is commonly used to treat anemia in CKD patients, and is effective and well tolerated worldwide. However, the interaction between iron and VC remains controversial, and the underlying mechanisms are yet to be clarified. In the present study, ex vivo normal rat aortic rings were cultured with various concentrations of phosphate and IS, and the levels of calcium and iron depositions, oxidative injury, as well as phenotypic marker genes were detected. To the best of our knowledge, the present study is the first to report that IS is a double-edged sword in high phosphate media-induced VC which not only alleviates VC in a dose-dependent manner but also leads to iron overload in vasculature when in high concentration. IS is a promising agent for VC prevention in patients with hyperphosphatemia and iron deficiency. Meanwhile, the appropriate blood concentration of IS in patients with hyperphosphatemia needs to be explored clinically.
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http://dx.doi.org/10.1007/s00223-020-00804-1DOI Listing
January 2021

Key Role of CD151-integrin Complex in Lung Cancer Metastasis and Mechanisms Involved.

Curr Med Sci 2020 Dec 11;40(6):1148-1155. Epub 2021 Jan 11.

Division of Cardiology, Department of Internal Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.

Tetraspanin CD151 was found to be upregulated in malignant cell types and has been identified as a tumor metastasis promoter. In this study, we aimed to examine the role of the CD151-integrin complex in lung cancer metastasis and the underlying mechanisms. CD151 QRD →AAA mutant was generated and used to transfect A549 human lung adenocarcinoma cells. We found that there was no significant difference in CD151 protein expression between CD151 and CD151-AAA mutant groups. In vitro, CD151-AAA mutant delivery abrogated the migration and invasion of A549 cells, which was promoted by CD151 gene transfer. Furthermore, CD151-AAA delivery failed to activate FAK and p130Cas signaling pathways. Western blot and immunohistochemical staining showed strong CD151 expression in lung cancerous tissues but not in adjacent normal tissues. Increased level of CD151 protein was observed in 20 of the patients and the positive rate of CD151 protein in specimens was 62.5% (20/32). In addition, CD151 was co-localized with α3 integrin at the cell-cell contact site in carcinoma tissues. These results suggested that the disruption of the CD151-α3 integrin complex may impair the metastasis-promoting effects and signaling events induced by CD151 in lung cancer. Our findings identified a key role for CD151-α3 integrin complex as a promoter in the lung cancer.
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http://dx.doi.org/10.1007/s11596-020-2297-7DOI Listing
December 2020

LncRNA DANCR represses Doxorubicin-induced apoptosis through stabilizing MALAT1 expression in colorectal cancer cells.

Cell Death Dis 2021 01 6;12(1):24. Epub 2021 Jan 6.

Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Center for Stem Cell Biology and Tissue Engineering, Zhongshan School of Medicine, Sun Yat-Sen University, 510080, Guangzhou, China.

Long non-coding RNA (lncRNA) DANCR has been reported to participate in key processes such as stem cell differentiation and tumorigenesis. In a high throughput screening for lncRNAs involved in Doxorubicin-induced apoptosis, we found DANCR was suppressed by Doxorubicin and it acted as an important repressor of apoptosis in colorectal cancer. Further studies demonstrated that DANCR promoted the oncogenic lncRNA MALAT1 expression via enhancing the RNA stability of MALAT1 to suppress apoptosis. MALAT1 could efficiently mediate the suppressive function of DANCR on apoptosis. Mechanistic studies found the RNA-binding protein QK served as an interacting partner of both DANCR and MALAT1, and the protein level of QK was subjected to the regulation by DANCR. Furthermore, QK was able to modulate the RNA stability of MALAT1, and the interaction between QK and MALAT1 was controlled by DANCR. In addition, QK could mediate the function of DANCR in regulating the expression of MALAT1 and suppressing apoptosis. These results revealed DANCR played a critical role in Doxorubicin-induced apoptosis in colorectal cancer cells, which was achieved by the interaction between DANCR and QK to enhance the expression of MALAT1.
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http://dx.doi.org/10.1038/s41419-020-03318-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7791116PMC
January 2021

Preparation of Alkaline Polyelectrolyte Membrane Based on Quaternary Ammonium Salt-Modified Cellulose and Its Application in Zn-Air Flexible Battery.

Polymers (Basel) 2020 Dec 22;13(1). Epub 2020 Dec 22.

Advanced Materials Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China.

In this study, a type of alkaline solid polyelectrolyte (ASPE) membrane was developed via the introduction of microcrystalline cellulose (MCC) and its modified product (QMCC) into the polyvinyl alcohol (PVA) matrix. In this process, green NaOH/urea-based solvent was used to achieve a good dispersion of MCC in the PVA matrix; meanwhile, the OH groups in the NaOH/urea-based solvent provided an alkaline environment for good ion conductivity. Compared to the MCC-incorporated ASPE, further improved conductivity was achieved when the MCC was modified with quantitative quaternary ammonium salt. TGA showed that the addition of QMCC improved the water retention of the matrix, which was beneficial to the OH conduction in the system. Compared to the control (50 mS cm), a maximum conductivity of 238 mS cm was obtained after the incorporation of QMCC in the PVA matrix. Moreover, the tensile strength of the polymer electrolyte were also significantly increased with the addition of QMCC. Finally, this developed ASPE membrane was used in assembling a flexible Zn-air battery and showed a promising potential in the development of flexible electronic devices.
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http://dx.doi.org/10.3390/polym13010009DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7792967PMC
December 2020

Distinct Community Assembly Processes of Abundant and Rare Soil Bacteria in Coastal Wetlands along an Inundation Gradient.

mSystems 2020 Dec 22;5(6). Epub 2020 Dec 22.

State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, China

Microbial communities commonly consist of a large number of rare taxa (RT) and few abundant taxa (AT), and it is important to identify the differences of the community assembly processes between RT and AT in response to environmental changes. However, the community assembly processes governing AT and RT in coastal wetland soils along an inundation gradient remain elusive. Here, an mesocosm, with continuous inundation gradients and native mangrove or exotic cordgrass , was established to determine the patterns and driving factors of community turnover and assembly processes of AT and RT. We found that RT exhibited a remarkably lower turnover rate than AT, and the niche breadth of RT was significantly narrower than that of AT. In comparison with AT, RT presented stronger phylogenetic signals for ecological preferences across environmental gradients. Null model analyses revealed that RT were more phylogenetically clustered and primarily governed by homogeneous selection, while AT were more overdispersed and dominated by dispersal limitation. Soil water content was the most decisive factor for community turnover and assembly processes of both AT and RT. Structural equation modeling analysis showed that RT were strongly associated with biomass rather than biomass, suggesting a strong relationship between RT and the growth of mangrove Overall, our study revealed distinct assembly processes of soil AT and RT communities in coastal wetlands, which is crucial for mechanistic understanding of the establishment and maintenance of soil microbial diversity in coastal wetlands under conditions of global environmental changes. Coastal wetlands are one of the important ecosystems that play a crucial role in the regulation of climate change. Rare taxa (RT) exist in one habitat along with abundant taxa (AT). In this study, we found that RT exhibited narrower niche breadth and stronger phylogenetic signals than AT. Null model analyses showed that RT were more phylogenetically clustered and primarily governed by homogeneous selection, while AT were more overdispersed and dominated by dispersal limitation. Revealing the differences in the community assembly processes between AT and RT in coastal wetlands is critical to understand the establishment and maintenance of soil microbial diversity in coastal wetlands with regard to environmental changes.
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http://dx.doi.org/10.1128/mSystems.01150-20DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7762797PMC
December 2020

Biosorbent with superhydrophobicity and superoleophilicity for spilled oil removal.

Ecotoxicol Environ Saf 2021 Feb 22;209:111803. Epub 2020 Dec 22.

School of Environment, South China Normal University, Guangzhou Higher Education Mega Center, Guangzhou 510006, PR China. Electronic address:

The development of efficient and sustainable sorbents for emergent oil cleanup has attracted tremendous attention. In this study, the feasibility of enzymatic grafting of octadecylamine (ODA) on corn stalk pith (CSP) by laccase-TEMPO (2,2,6,6-tetramethylpiperidine-1-oxyl) treatment for enhanced oil-water separation was investigated. The dynamic contact angle tests suggest that the modified CSP (LCSP) had higher hydrophobicity (WCA, 157.2˚) and lipophilicity (OCA, 0˚) than the CSP did. In addition, the introduction of ODA onto the surface of modified CSP was verified by a variety of characterization techniques including SEM, FT-IR, and XPS. Compared with the control, laccase-TEMPO treatment of CSP coupled with octadecylamine grafting greatly improved the oil sorption capacity from 13.24 g/g to 44.25 g/g, while substantially reduced the water sorption capacity from 15.52 g/g to 2.76 g/g. LCSP has fast kinetic (sorption equilibrium reached before 60 min) and high fits to the pseudo-second-order kinetic model. The results obtained in this study reveal the feasibility of using Laccase-TEMPO treatment to graft the ODA onto the surface of CSP, thereby enhancing the rate and capacity of oil separation from oily water. The method and sorbent developed in this study hold promise for green, simple and cost-effective oil cleanup during oil spillage emergency events.
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http://dx.doi.org/10.1016/j.ecoenv.2020.111803DOI Listing
February 2021

Solution pH affects single, sequential and binary systems of sulfamethoxazole and cadmium adsorption by self-assembled cellulose: Promotion or inhibition?

J Hazard Mater 2021 01 23;402:124084. Epub 2020 Sep 23.

Department of Transportation and Environment, Shenzhen Institute of Information Technology, Shenzhen 518172, PR China. Electronic address:

A new self-assembled cellulose (SACS) containing multi-functional amine, carboxyl and hydroxyl groups was successfully obtained through etherification, cross-linking and grafting processes. Then, the adsorption of sulfamethoxazole (SMZ) and Cd(II) onto SACS at pH values of 3, 5.7 and 7.5 was systematically investigated by batch experiments of single, sequential and binary systems, characterization and density functional theory (DFT) calculations. The presence of Cd(II) decreased the adsorption of SMZ because of hydrophilic site competition, while SMZ inversely increased the adsorption of Cd(II), which was attributed to bridging and especially to electrostatic shielding effects; moreover, both the inhibitory and synergistic effects were more obvious in the binary system and at a pH of 7.5. There was a dynamic balance between the inhibitory and synergistic effects that depended on the system, pH value and concentration ratio. DFT results further indicated that SMZ more easily coordinated with Cd(II) at sulfonyl oxygen and nitrogen sites, and the cationic bridge of Cd(II) with SMZ mainly occurred in the sequential system. Moreover, a complexation-decomplexation-complexation balance of SMZ and Cd(II) probably occurred in the binary system.
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http://dx.doi.org/10.1016/j.jhazmat.2020.124084DOI Listing
January 2021

Reversible Mechanochromic Luminescence of Tetranuclear Cuprous Complexes.

Inorg Chem 2020 Dec 18;59(23):17213-17223. Epub 2020 Nov 18.

Department of Materials, Metallurgy and Chemistry, Jiangxi University of Science and Technology, Ganzhou 341000, People's Republic of China.

Mechanochromic luminescence materials have attracted rapidly growing interest. Nevertheless, the designed synthesis of such materials remains a challenge, and there have been few examples based on weak intramolecular interactions. Herein, we report a new approach for preparing mechanochromic luminescence materials of Cu(I) complexes, i.e., constructing a photoluminescence system that bears a large coplanar multinuclear Cu(I) unit showing weak intramolecular π···π interactions with the planar rings of the coordinated ligands in the molecule. Using it, a series of novel mechanochromic luminescent tetranuclear Cu(I) complexes have been successfully designed and synthesized. As revealed by single-crystal X-ray crystallography, these Cu(I) complexes share an identical {Cu[μ-η(N,N),η(N),η(N)-pyridyltetrazole]} planar fragment whose coplanar pyridyl rings exhibit weak intramolecular π···π interactions with the phenyl rings of the coordinated phosphine ligands in the molecule. All of these Cu(I) complexes exhibit reversible mechanochromic luminescence, which can be attributed to the change in the rigidity of the molecular structure resulting from the disruption and restoration of intramolecular π···π interactions between the pyridyl and phenyl rings triggered by grinding and CHCl vapor, as supported by powder X-ray diffraction and Fourier transform infrared spectrometry. In addition, the results might provide a new route for developing mechanochromic luminescence materials of Cu(I) complexes for intelligent responsive luminescent devices.
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http://dx.doi.org/10.1021/acs.inorgchem.0c02445DOI Listing
December 2020

Serum metabolomic profiling reveals important difference between infants with and without subsequent recurrent wheezing in later childhood after RSV bronchiolitis.

APMIS 2021 Mar 25;129(3):128-137. Epub 2020 Dec 25.

Department of Medical Microbiology, Capital Medical University, Beijing, China.

We aimed to use serum metabolomics to discriminate infants with severe respiratory syncytial virus (RSV) bronchiolitis who later developed subsequent recurrent wheezing from those who did not and to investigate the relationship between serum metabolome and host immune responses with regard to the subsequent development of recurrent wheezing. Fifty-one infants who were hospitalized during an initial episode of severe RSV bronchiolitis at 6 months of age or less were included and followed for up to the age of 3 years. Of them, 24 developed subsequent recurrent wheezing and 27 did not. Untargeted serum metabolomics was performed by ultraperformance liquid chromatography coupled with high-resolution mass spectrometry (UPLC-MS/MS). Cytokines were measured by multiplex immunoassay. Difference in serum metabolomic profiles was observed between infants who developed recurrent wheezing and those who did not. L-lactic acid level was significantly higher in infants with recurrent wheezing than those without. Pyrimidine metabolism, glycerophospholipid metabolism, and arginine biosynthesis were identified as the most significant changed pathways between the two groups. Moreover, L-lactic acid level was positively associated with serum CXCL8 level. This exploratory study showed that differential serum metabolic signatures during severe RSV bronchiolitis in early infancy were associated with the development of subsequent recurrent wheezing in later childhood.
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http://dx.doi.org/10.1111/apm.13095DOI Listing
March 2021

The clinical efficacy and adverse effects of Entecavir plus Thymosin alpha-1 combination therapy versus Entecavir Monotherapy in HBV-related cirrhosis: a systematic review and meta-analysis.

BMC Gastroenterol 2020 Oct 19;20(1):348. Epub 2020 Oct 19.

Department of Pharmacy, Daping Hospital, Army Medical University, Chongqing, 400042, China.

Background: Previous studies have demonstrated the benefits of thymosin alpha-1 (Tα1) in anti-virus, immunological enhancement and anti-inflammation. However, it is controversial about the efficacy and safety of entecavir (ETV) plus Tα1 combination therapy versus ETV monotherapy in cirrhotic patients with hepatitis B virus (HBV) infection.

Methods: The systematic review and meta-analysis of randomized clinical trials (RCTs) were performed to evaluate the efficacy and safety of ETV plus Tα1 combination therapy versus ETV monotherapy in HBV-related patients with cirrhosis. We performed a systematic literature search via PubMed, Web of Science, Cochrane Central Register of Controlled Trials (CENTRAL), EMBASE, China National Knowledge Infrastructure (CNKI), Chinese Science and Technology Journals Database (VIP), and Chinese Biological Medicine database (CBM). Relative risk (RR) and standardized mean difference (SMD) with a fixed- or random- effect model were calculated. Heterogeneity was assessed through a Cochrane Q-test and I values.

Results: Seven RCTs involving 1144 subjects were included in the systematic review and meta-analysis. Compared with ETV monotherapy, ETV plus Tα1 combination therapy led to a higher complete response (RR = 1.18; 95% CI, 1.07-1.30). In post treatment for 24 weeks, the HBV DNA undetectable rate and HBeAg loss rate were higher in ETV plus Tα1 group than in ETV alone group (RR = 1.91; 95% CI, 1.56-2.35; RR = 2.05; 95% CI, 1.62-2.60). However, after 48 and 52 weeks of treatment, there was no significant difference between the combination therapy and ETV monotherapy (RR = 1.07; 95% CI, 0.96-1.18; RR = 1.17; 95% CI, 0.89-1.55). At week 52 of treatment, the HBsAg loss rate of ETV plus Tα1 group was no significance with that of ETV alone group (RR = 1.03; 95% CI, 0.15-7.26). In comparison with ETV alone, the some biochemical parameters and liver fibrosis were obviously improved by ETV plus Tα1, and there was significant heterogeneity. In addition, the number of adverse events was significantly reduced by ETV plus Tα1, compared to ETV alone (RR = 0.48; 95% CI, 0.24-0.95).

Conclusions: ETV plus Tα1 might lead to a higher clinical response and a lower comprehensive adverse reaction rate in HBV-related patients with cirrhosis, compared to ETV alone. However, the whole patients included in this meta-analysis were from Chinese mainland, so that more worldwide RCTs with a larger sample size are needed to verify the current findings.
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http://dx.doi.org/10.1186/s12876-020-01477-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7574490PMC
October 2020

Childhood Abuse Classes for Incarcerated Men and Women: Are There Unique Gender Patterns in Abuse Classes?

J Interpers Violence 2020 Oct 18:886260520965974. Epub 2020 Oct 18.

Rutgers, The State University of New Jersey, NJ, USA.

Childhood adversity is predictive of poorer health and behavioral health outcomes in adulthood. Males and females are known to experience different types of childhood adversity, with females experiencing more sexual and emotional harm in childhood. Latent class analysis (LCA) has been used to identify patterns among types of childhood adversity. These studies have constructed class structures using single gender or blended gender samples. Class structures based on blended gender samples, however, may misrepresent the nuances of gender-specific adversity histories through averaging, potentially distorting the relative need for gender-specific types of intervention. This study investigated whether latent class structures of childhood abuse are equivalent for incarcerated males and females. Our sample included 4,204 residents (3,986 males, 218 females) drawn from a single prison system. Residents completed an hour-long audio computer-assisted self-interview that included questions on 10 types of childhood abuse, depression, and anxiety symptoms, the Beck Hopelessness Scale (BHS), Buss-Perry Aggression Questionnaire, and Criminal Sentiments Scale-Modified (CSS-M). Overall, female residents were both more likely to experience childhood abuse and have more extensive victimization experiences. Small subgroups of males, however, had even more extensive victimization experiences. Abuse patterns for males and females, while optimally clustering in four classes, are rather unique, especially for higher abuse classes, in terms of distribution of membership and types of abuse. These differences may matter in terms of identifying the relative need for therapeutic intervention among incarcerated males and females and targeting those interventions in ways that reflect the gradient and density of therapeutic need. The next step is to test whether using blended or gendered latent class structures matters in terms of predicting outcomes, such as prison-based behavioral health problems, suicidality, and victimization.
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http://dx.doi.org/10.1177/0886260520965974DOI Listing
October 2020

Dramatic change of bacterial assembly process and co-occurrence pattern in Spartina alterniflora salt marsh along an inundation frequency gradient.

Sci Total Environ 2021 Feb 28;755(Pt 1):142546. Epub 2020 Sep 28.

State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, 71 East Beijing Road, Nanjing 210008, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:

Exotic Spartina alterniflora has become widely distributed along most of the coastlines in China in a wide range of inundation frequencies. However, the assembly processes and co-occurrence patterns of the bacterial community in S. alterniflora wetlands under different inundation frequencies remain elusive. In this study, an in-situ mesocosm was established to investigate the changes in soil bacterial community. We found that soil water content was the most decisive factor in influencing the bacterial community. Balanced variation, rather than abundance gradients, accounted for the major shifts in bacterial communities and was significantly and positively correlated with the changes in water content, suggesting that species substitution was facilitated by the increased water content. Deterministic processes were dominant in community assembly, and a large degree of change in water content increased variable selection. Co-occurrence network revealed that increasing water content significantly decreased the average degree and the relative abundance of keystone species, resulting in a network with less complexity. Structural equation modelling suggests that increasing inundation frequency has strong impacts on bacterial community, primarily by altering water content, network degree, and the relative abundance of keystone species. Overall, our results illustrate that increasing inundation frequency significantly influences the bacterial community assembly processes and co-occurrence patterns.
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http://dx.doi.org/10.1016/j.scitotenv.2020.142546DOI Listing
February 2021

Study on the Synthesis and Thermal Stability of Silicone Resin Containing Trifluorovinyl Ether Groups.

Polymers (Basel) 2020 Oct 5;12(10). Epub 2020 Oct 5.

School of Materials Science and Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, China.

Silicone resin is a high-temperature resistant material with excellent performance. The improvement of its thermal stability has always been the pursuit of researchers. In this paper, a sequence of silicone resins containing trifluorovinyl ether groups were prepared by the co-hydrolysis-polycondensation of methyl alkoxysilane monomers and {4-[trifluorovinyl(oxygen)]phenyl}methyldiethoxysilane. The structures of the silicone resins were characterized by FT-IR and H NMR. The curing process of them was studied by DSC and FT-IR spectra, and results showed that the curing of the resins included the condensation of the Si-OH groups and the [2 + 2] cyclodimerization reaction of the TFVE groups, which converted to perfluorocyclobutane structure after curing. The thermal stability and thermal degradation behavior of them was studied by TGA and FT-IR spectra. Compared with the pure methyl silicone resin, silicone resins containing TFVE groups showed better thermal stability under both N and air atmosphere. Their hydrophobic properties were characterized by contact angle test. Results showed that PFCB structure also improved the hydrophobicity of the silicone resin.
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http://dx.doi.org/10.3390/polym12102284DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7601899PMC
October 2020

Sorption of pharmaceuticals and personal care products on soil and soil components: Influencing factors and mechanisms.

Sci Total Environ 2021 Jan 26;753:141891. Epub 2020 Aug 26.

College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi 712100, China; Key Laboratory of Plant Nutrition and the Agro-environment in Northwest China, Ministry of Agriculture, Yangling, Shaanxi 712100, China. Electronic address:

The sorption of pharmaceuticals and personal care products (PPCPs) on soil and soil components makes an important contribution to the fate, migration and bioavailability of PPCPs. Previous reviews have mostly focused on the sorption of PPCPs on single soil components (e.g., minerals and soil organic matter). However, the sorption of PPCPs within the whole soil system has not been systematically analyzed. This paper reviews the recent progress on PPCP sorption on soil and soil components. We have evaluated the sorption of a wide range of PPCPs in research fields that are usually considered in isolation (e.g., humic acids (HAs), montmorillonite, kaolinite, and goethite), and established a bridge between PPCPs and sorbent. The sorption mechanisms of PPCPs, e.g., cation exchange, surface complexation, electrostatic interaction and hydrogen bonding, are discussed and critically evaluated. We also assessed the influence of environmental factors (pH, ionic strength, organic matter and temperature) on sorption. This review summarizes the knowledge of PPCPs sorption on soil gained in recent years, which can provide new strategies for solving the problem of antibiotic pollution.
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http://dx.doi.org/10.1016/j.scitotenv.2020.141891DOI Listing
January 2021

Alteration of the fatty acid composition of Brassica napus L. via overexpression of phospholipid: Diacylglycerol acyltransferase 1 from Sapium sebiferum (L.) Roxb.

Plant Sci 2020 Sep 12;298:110562. Epub 2020 Jun 12.

Faculty of Bioscience and Biotechnology of Central South University of Forestry and Technology, 410004, Changsha, China; Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, 410018, Changsha, China. Electronic address:

Sapium sebiferum (L.) Roxb. plays an important role in traditional Chinese medicine and is one of major woody oil tree in China. Phospholipid: diacylglycerol acyltransferase 1 (PDAT1), as an important catalytic enzyme for the formation of triacylglycerol (TAG), is mainly responsible for the transfer of an acyl group from the sn-2 position of phospholipids to the sn-3 position of sn-1, 2-diacylglycerol (DAG) to produce TAG and sn-1 lysophospholipids. The importance of PDAT1 in triacylglycerol biosynthesis has been illustrated in previous research, and at least 67 PDAT1 sequences have been identified from 31 organisms. However, little is known about the gene encoding PDAT1 in S. sebiferum (SsPDAT1), which is involved in seed oil biosynthesis. To explore the functional characteristics of SsPDAT1, we cloned and analyzed the full-length cDNA in the coding region of SsPDAT1, which consists of 2040 bp and encodes a putative protein of 680 amino acid (aa) residues. Thin-layer chromatography (TLC) analysis showed that recombinant SsPDAT1 could restore TAG accumulation in TAG-deficient mutant yeast (Saccharomyces cerevisiae) H1246, which revealed the enzyme activity of SsPDAT1. Moreover, transgenic Brassica napus L. W10 plants overexpressing SsPDAT1 showed significant increases of 19.6-28.9 % in linoleic acid levels but decreases of 27.3-37.1 % in linolenic acid. Furthermore, the total oil content increased by 8.1 %-10.8 % in SsPDAT1 transgenic seeds. These results confirmed the role of SsPDAT1 in stabilizing oil biosynthesis and suggested that SsPDAT1 could be exploitable to specifically regulate the oil composition of plants. These experimental results provide a new concept that may enable the industrial development of plants with high-linoleic-acid oil through overexpression of SsPDAT1 in S. sebiferum L.
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http://dx.doi.org/10.1016/j.plantsci.2020.110562DOI Listing
September 2020