Publications by authors named "Dan He"

373 Publications

[An epidemiological survey of laryngopharyngeal refux disease in otorhinolaryngology head and neck surgery clinics in Chongqing area].

Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2021 Apr;35(4):351-354;359

Department of Otolaryngology Head and Neck Surgery,Affiliated Hospital of Southwest Medical University,Luzhou,646000,China.

To investigate the prevalence and affecting factors of laryngopharyngeal reflux disease(LPRD) in otolaryngology head and neck surgery in Chongqing,and to provide a basis for the clinical diagnosis and therapy of LPRD. Multi-center cross-sectional survey method and systematic sampling method were used to select patients at fifteen hospitals in Chongqing from August to November in 2019. Then reflux symptom index(RSI) was investigated. At the same time, the information of the relevant dietary habits, including smoking and drinking, spicy diet, high-fat diet, and satiety was collected. Moreover, the factors related to LRPD(gender, age, symptoms, diet and lifestyle) were analyzed. The composition ratio of LPRD was 11.90%(385/3234) in 3234 effective questionnaires and 385 positive ones. The composition ratio was 12.55%(173/1378) in men and 11.42%(212/1856) in women. The difference between the two groups was statistically significant(<0.05). The difference in composition ratio among different age groups was statistically significant(<0.05), with the highest composition ratio between 40 and 59 years old(170/1390). Constant throat-clearing(symptom 2) and globus sensation(symptom 8) were most correlated with LPRD. Logistic regression analysis showed that spicy diet, over eating, and smoking were highly related to LPRD. Globus sensation and constant throat-clearing are the most common symptoms in LPRD patients. Spicy diet, over eating, and smoking are risk factors for LPRD.
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http://dx.doi.org/10.13201/j.issn.2096-7993.2021.04.015DOI Listing
April 2021

Prioritization of schizophrenia risk genes from GWAS results by integrating multi-omics data.

Transl Psychiatry 2021 Mar 17;11(1):175. Epub 2021 Mar 17.

Department of Medical Research Center, Sun Yat-Sen Memorial Hospital, Guangzhou, China.

Schizophrenia (SCZ) is a polygenic disease with a heritability approaching 80%. Over 100 SCZ-related loci have so far been identified by genome-wide association studies (GWAS). However, the risk genes associated with these loci often remain unknown. We present a new risk gene predictor, rGAT-omics, that integrates multi-omics data under a Bayesian framework by combining the Hotelling and Box-Cox transformations. The Bayesian framework was constructed using gene ontology, tissue-specific protein-protein networks, and multi-omics data including differentially expressed genes in SCZ and controls, distance from genes to the index single-nucleotide polymorphisms (SNPs), and de novo mutations. The application of rGAT-omics to the 108 loci identified by a recent GWAS study of SCZ predicted 103 high-risk genes (HRGs) that explain a high proportion of SCZ heritability (Enrichment = 43.44 and [Formula: see text]). HRGs were shown to be significantly ([Formula: see text]) enriched in genes associated with neurological activities, and more likely to be expressed in brain tissues and SCZ-associated cell types than background genes. The predicted HRGs included 16 novel genes not present in any existing databases of SCZ-associated genes or previously predicted to be SCZ risk genes by any other method. More importantly, 13 of these 16 genes were not the nearest to the index SNP markers, and them would have been difficult to identify as risk genes by conventional approaches while ten out of the 16 genes are associated with neurological functions that make them prime candidates for pathological involvement in SCZ. Therefore, rGAT-omics has revealed novel insights into the molecular mechanisms underlying SCZ and could provide potential clues to future therapies.
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http://dx.doi.org/10.1038/s41398-021-01294-xDOI Listing
March 2021

Novel therapies for malignant pleural effusion: Anti‑angiogenic therapy and immunotherapy (Review).

Int J Oncol 2021 Mar 22;58(3):359-370. Epub 2021 Jan 22.

Institute of Drug Clinical Trial/GCP Center, Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, P.R. China.

Patients with a variety of malignancies can develop malignant pleural effusion (MPE). MPE can cause significant symptoms and result in a marked decrease in quality of life and a poor prognosis. MPE is primarily considered as an immune and vascular manifestation of pleural metastases. In the present review, the existing evidence supporting the applicability of anti‑angiogenic therapy and immunotherapy for the treatment of MPE was summarized. Patients with MPE have benefited from anti‑angiogenic agents, including bevacizumab and endostar; however, no relevant prospective phase III trial has, thus far, specifically analyzed the benefit of anti‑angiogenic therapy in MPE. Immunotherapy for MPE may be sufficient to turn a dire clinical situation into a therapeutic advantage. Similar to anti‑angiogenic therapy, more clinical data on the efficiency and safety of immunotherapy for controlling MPE are urgently required. The combined use of anti‑angiogenic therapy and immunotherapy may be a promising strategy for MPE, which requires to be further understood.
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http://dx.doi.org/10.3892/ijo.2021.5174DOI Listing
March 2021

The COMPASS-like complex modulates fungal development and pathogenesis by regulating H3K4me3-mediated targeted gene expression in Magnaporthe oryzae.

Mol Plant Pathol 2021 Apr 8;22(4):422-439. Epub 2021 Feb 8.

Beijing Key Laboratory of New Technology in Agricultural Application, National Demonstration Center for Experimental Plant Production Education, Beijing University of Agriculture, Beijing, China.

Histone-3-lysine-4 (H3K4) methylation is catalysed by the multiprotein complex known as the Set1/COMPASS or MLL/COMPASS-like complex, an element that is highly evolutionarily conserved from yeast to humans. However, the components and mechanisms by which the COMPASS-like complex targets the H3K4 methylation of plant-pathogenic genes in fungi remain elusive. Here we present a comprehensive analysis combining biochemical, molecular, and genome-wide approaches to characterize the roles of the COMPASS-like family in the rice blast fungus Magnaporthe oryzae, a model plant pathogen. We purified and identified six conserved subunits of COMPASS from M. oryzae: MoBre2 (Cps60/ASH2L), MoSpp1 (Cps40/Cfp1), MoSwd2 (Cps35), MoSdc1 (Cps25/DPY30), MoSet1 (MLL/ALL), and MoRbBP5 (Cps50), using an affinity tag on MoBre2. We determined the sequence repeat in dual-specificity kinase splA and ryanodine receptors domain of MoBre2 can interact directly with the DPY30 domain of MoSdc1 in vitro. Furthermore, we found that deletion of the genes encoding COMPASS subunits of MoBre2, MoSPP1, and MoSwd2 caused similar defects regarding invasive hyphal development and pathogenicity. Genome-wide profiling of H3K4me3 revealed that it has remarkable co-occupancy at the transcription start site regions of target genes. Significantly, these target genes are often involved in spore germination and pathogenesis. Decreased gene expression caused by the deletion of MoBre2, MoSwd2, or MoSpp1 was highly correlated with a decrease in H3K4me3. These results suggest that MoBre2, MoSpp1, and MoSwd2 function as a whole COMPASS complex, contributing to fungal development and pathogenesis by regulating H3K4me3-targeted genes in M. oryzae.
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http://dx.doi.org/10.1111/mpp.13035DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7938624PMC
April 2021

Roles of peptidyl-prolyl isomerase Pin1 in disease pathogenesis.

Theranostics 2021 19;11(7):3348-3358. Epub 2021 Jan 19.

The Second Affiliated Hospital of Chengdu Medical College, China National Nuclear Corporation 416 Hospital, Chengdu, Sichuan, China.

Pin1 belongs to the peptidyl-prolyl cis-trans isomerases (PPIases) superfamily and catalyzes the cis-trans conversion of proline in target substrates to modulate diverse cellular functions including cell cycle progression, cell motility, and apoptosis. Dysregulation of Pin1 has wide-ranging influences on the fate of cells; therefore, it is closely related to the occurrence and development of various diseases. This review summarizes the current knowledge of Pin1 in disease pathogenesis.
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http://dx.doi.org/10.7150/thno.45889DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7847688PMC
January 2021

Identification of Genes with Altered Methylation and Its Role in Early Diagnosis of Sepsis-Induced Acute Respiratory Distress Syndrome.

Int J Gen Med 2021 25;14:243-253. Epub 2021 Jan 25.

Department of Emergency Medicine, The Second Affiliated Hospital of Guangxi Medical University, Nanning 530007, People's Republic of China.

Purpose: Early diagnosis of sepsis-induced acute respiratory distress syndrome (ARDS) is critical for effective treatment. We aimed to identify early stage biomarkers.

Materials And Methods: Differentially expressed genes were identified in whole blood samples from patients with sepsis or ARDS based on the Gene Expression Omnibus (GEO) datasets GSE32707, GSE54514 and GSE10361. Functional enrichment analysis explored the biological characteristics of differentially expressed genes. Genes with high functional connectivity based on a protein-protein interaction network were marked as hub genes, which were validated using the GEO dataset GSE76293, and a gene set variation analysis index (GSVA) was assigned. Diagnostic and predictive ability of the hub genes were assessed by receiver operating characteristic (ROC) curve analysis. DNA methylation levels of hub genes were quantified using the GEO dataset GSE67530.

Results: Forty-one differentially expressed genes were shared between sepsis-specific and ARDS-specific datasets. MAP2K2 and IRF7 functional activity was highly connected in sepsis-induced ARDS. Hub genes included RETN, MVP, DEFA4, CTSG, AZU1, FMNL1, RBBP7, POLD4, RIN3, IRF7. ROC curve analysis of the hub gene GSVA index showed good diagnostic ability in sepsis or ARDS. Among genes related to sepsis-induced ARDS, 17 were differentially methylated. Principal component analysis and heatmaps indicated that gene methylation patterns differed significantly between ARDS patients and controls.

Conclusion: We identified a genetic profile specific to early-stage sepsis-induced ARDS. The abnormal expression of these genes may be caused by hypomethylation, which may serve as a biomarker for early diagnosis of ARDS.
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http://dx.doi.org/10.2147/IJGM.S287960DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7847772PMC
January 2021

MiR-29b-3p aggravates cardiac hypoxia/reoxygenation injury via targeting PTX3.

Authors:
Dan He Lei Yan

Cytotechnology 2021 Feb 5;73(1):91-100. Epub 2021 Jan 5.

Department of Thoracic Surgery, Hubei Provincial Hospital of Integrated Chinese & Western Medicine, Wuhan, 430015 Hubei China.

Our current research aimed to decipher the role and underlying mechanism with regard to miR-29b-3p involving in myocardial ischemia/reperfusion (I/R) injury. In the present study, cardiomyocyte H9c2 cell was used, and hypoxia/reoxygenation (H/R) model was established to mimic the myocardial I/R injury. The expressions of miR-29b-3p and pentraxin 3 (PTX3) were quantified deploying qRT-PCR and Western blot, respectively. The levels of LDH, TNF-α, IL-1β and IL-6 were detected to evaluate cardiomyocyte apoptosis and inflammatory response. Cardiomyocyte viability and apoptosis were examined employing CCK-8 assay and flow cytometry, respectively. Verification of the targeting relationship between miR-29b-3p and PTX3 was conducted using a dual-luciferase reporter gene assay. It was found that miR-29b-3p expression in H9c2 cells was up-regulated by H/R, and a remarkable down-regulation of PTX3 expression was demonstrated. MiR-29b-3p significantly promoted of release of inflammatory cytokines of H9c2 cells, and it also constrained the proliferation and promoted the apoptosis of H9c2 cells. Additionally, PTX3 was inhibited by miR-29b-3p at both mRNA and protein levels, and it was identified as a direct target of miR-29b-3p. PTX3 overexpression could reduce the inflammatory response, increase the viability of H9c2 cells, and inhibit apoptosis. Additionally, PTX3 counteracted the function of miR-29b-3p during the injury of H9c2 cells induced by H/R. In summary, miR-29b-3p was capable of aggravating the H/R injury of H9c2 cells by repressing the expression of PTX3.
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http://dx.doi.org/10.1007/s10616-020-00446-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7817735PMC
February 2021

Optimization of Porphyran Extraction from by Response Surface Methodology and Its Lipid-Lowering Effects.

Mar Drugs 2021 Jan 23;19(2). Epub 2021 Jan 23.

College of Life and Environmental Science, Wenzhou University, Wenzhou 325035, China.

Macroalgae polysaccharides are phytochemicals that are beneficial to human health. In this study, response surface methodology was applied to optimize the extraction procedure of porphyran (PYP). The optimum extraction parameters were: 100 °C (temperature), 120 min (time), and 29.32 mL/g (liquid-solid ratio), and the maximum yield of PYP was 22.15 ± 0.55%. The physicochemical characteristics of PPYP, purified from PYP, were analyzed, along with its lipid-lowering effect, using HepG2 cells and larvae. PPYP was a β-type sulfated hetero-rhamno-galactan-pyranose with a molecular weight of 151.6 kDa and a rhamnose-to-galactose molar ratio of 1:5.3. The results demonstrated that PPYP significantly reduced the triglyceride content in palmitic acid (PA)-induced HepG2 cells and high-sucrose-fed larvae by regulating the expression of lipid metabolism-related genes, reducing lipogenesis and increasing fatty acid β-oxidation. To summarize, PPYP can lower lipid levels in HepG2 cells and larval fat body (the functional homolog tissue of the human liver), suggesting that PPYP may be administered as a potential marine lipid-lowering drug.
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http://dx.doi.org/10.3390/md19020053DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7911723PMC
January 2021

Advances in Fusarium drug resistance research.

J Glob Antimicrob Resist 2021 Mar 15;24:215-219. Epub 2021 Jan 15.

Department of Pathogenobiology, Jilin University Mycology Research Center, College of Basic Medical Sciences, Jilin University, Changchun, 130021, China. Electronic address:

Fusarium species cause many diseases in plants and humans, which results in a great number of economic losses every year. The management of plant diseases and related human diseases caused by Fusarium is challenging as many kinds of Fusarium may be intrinsically resistant to antifungal drugs, not to mention the fact that they can acquire drug resistance, which is common in clinical practice. To date, the drug resistance of Fusarium is mainly related to target alterations, drug efflux and biofilm formation. This article reviews recent studies related to the mechanism of Fusarium resistance, and summarizes the key molecules affecting resistance.
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http://dx.doi.org/10.1016/j.jgar.2020.12.016DOI Listing
March 2021

Study on the spatial-temporal differences and evolution of ecological security in the typical area of the Loess Plateau.

Environ Sci Pollut Res Int 2021 Jan 15. Epub 2021 Jan 15.

School of Environmental and Chemical Engineering, Xi'an polytechnic University, Xi'an, Shaanxi, China.

The development and utilization of energy in the Loess Plateau has caused a wide range of ecological security issues, and Yan'an has become a typical area for ecological security research on the Loess Plateau. Ecological security evaluation research can provide data support and scientific reference value for the sustainable development, which is of great significance to the overall social and economic development of the region. In this study, the pressure-state-response (PSR) model was used to establish the evaluation index system in the evaluation of ecological security in Yan'an region (YAR), then the fuzzy analytic hierarchy process (FAHP) was used to determine the internal index weight of each element, and finally the ecological security index value (ESI) was calculated. The GIS technology was used to simulate the distribution map of ecological security in YAR and then analyzed the temporal and spatial changes and evolution of ecological security in YAR. The results showed that from 1997 to 2016, the ecological security in the western part of Luochuan County and the eastern part of Yanchuan County was still very high, while the ecological security index was relatively low in the southern part of Huanglong County. The ecological security index of Baota District had increased significantly, from 1.85 in 1997 to 2.76 in 2016. The proportion of III and IV ecological security regions had increased significantly, and the ecological security of the entire YAR was generally in a good situation. This study could clarify the temporal and spatial characteristics of ecological security and provided some reference for the study of ecological security evolution in typical regions of the Loess Plateau.
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http://dx.doi.org/10.1007/s11356-021-12372-4DOI Listing
January 2021

Low temperature performance and sulfur resistance enhancement of Mn-Ce oxides supported on W-modified MWCNT for NH-SCR removal of NO.

J Air Waste Manag Assoc 2021 Feb 5:1-12. Epub 2021 Feb 5.

School of Land Resources and Environment, Key Laboratory of Agricultural Resource and Ecology in the Poyang Lake Basin of Jiangxi Province, Jiangxi Agricultural University , Nanchang, People's Republic of China.

To eliminate nitrogen oxides (NO), composite carrier-supported catalysts (Mn-Ce/MWCNT-W) and traditional catalysts (Mn-Ce/MWCNT and W-Mn-Ce/MWCNT) were prepared using an ultrasonic impregnation method that preformed low-temperature ammonia-selective catalytic reduction (SCR) removal of NO. The promotion effects of MWCNT-W composite carriers for low temperature SCR activities and SO tolerance of the catalysts were systematically investigated. Compared to traditional catalyst, Mn-Ce/MWCNT-W catalyst, with a 30% WO/MWCNT mass ratio, demonstrated improved SCR activity and high N-selectivity from 100°C to 200°C. A series of characterizations were carried out and it was found that there were more redox sites and the stronger the NH adsorption capacity over the composite carrier-supported catalysts than traditional catalysts. Also, with this composite carrier-supported catalyst, the improvement of SCR reaction was considered to be from the abundance of high valence state Mn and well dispersed active components. Notably, compared to traditional catalyst, the composite carrier-supported catalyst exhibited the stronger sulfur resistance. Thus, using MWCNT-W composite carriers to prepare Mn-Ce/MWCNT-W catalysts resulted in excellent NO conversion and SO resistance at low temperatures. : LT NH-SCR of NO is an effective way to remove NO from stationary sources. The physicochemical properties of the support not only affect a catalyst's LT SCR activity but also affect the catalyst's anti-poisoning performance. The modified carriers could promote active component dispersion, which is conducive to SCR reaction. However, for LT SCR reactions, few reports have addressed the design and preparation of composite carrier-supported catalysts. The goal of this study was to design and synthesize Mn-Ce/MWCNT-W catalysts and to observe the influence of composite support in Mn-based catalysts on LT SCR activity and sulfur resistance.
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http://dx.doi.org/10.1080/10962247.2021.1873205DOI Listing
February 2021

Infiltration of plasma cells in colorectal adenocarcinoma associated with autoimmune encephalitis.

Ann Clin Transl Neurol 2021 02 5;8(2):498-503. Epub 2021 Jan 5.

Priority Area Asthma and Allergy, Research Center Borstel, Airway Research Center North (ARCN), German Center for Lung Research (DZL), Borstel, Germany.

Paraneoplastic autoimmune encephalitis (PAE) represents a group of rare neurological syndromes associated with neoplastic diseases. Here, we report a case that multiple anti-neuronal antibodies were present in a patient with PAE who developed both small cell lung cancer and colorectal adenocarcinoma. Furthermore, the immunopathological investigation of the colorectal adenocarcinoma revealed the formation of abnormal neuronal antigens and a massive infiltration of plasma cells in the tumor tissue. These findings support the hypothesis that expression of neuronal antigens in neoplasm initiates autoimmune responses in PAE.
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http://dx.doi.org/10.1002/acn3.51283DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7886041PMC
February 2021

Catanionic Hybrid Lipid Nanovesicles for Improved Bioavailability and Efficacy of Chemotherapeutic Drugs.

Methods Mol Biol 2021 ;2211:57-68

Chongqing Research Center for Pharmaceutical Engineering, Chongqing Medical University, Chongqing, China.

Catanionic nanovesicles are attractive as a novel class of delivery vehicle because they can increase the stability, adsorption, and cellular uptake of a broad range of drugs. These hybrid lipid nanocarriers consist of solid and liquid lipids, which are biocompatible and biodegradable. Since liquid lipid is added to the nanocarrier, the lipids are present in a crystalline defect or amorphous structure state. As a result, hybrid lipid nanocarriers have a higher drug loading capability and suffer less drug leakage during preparation and storage compared to the pure lipid nanocarriers. Catanionic nanovesicles have been shown to increase stability, adsorption, cellular uptake, apoptosis induction, tumor cell cytotoxicity, and antitumorigenic effect, making it a highly desirable vehicle for drug delivery. For example, the anticancer compound curcumin (CC) have shown great promise to cure cancers such as lung cancer, breast cancer, stomach cancer, and colon cancer. However, like many potential antitumor drugs, CC on its own has poor water solubility, easy photodegradation, chemical instability, low bioavailability, rapid metabolism, and fast systematic clearance, which severely limits its clinical applications. In this chapter, we demonstrate the use of catanionic nanovesicles to improve the bioavailability and efficacy of CC for anticancer applications. This technique can be easily adapted for delivery and evaluation of other bioactive compounds.
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http://dx.doi.org/10.1007/978-1-0716-0943-9_5DOI Listing
March 2021

Microcystin-LR heterologous genetically engineered antibody recombinant and its binding activity improvement and application in immunoassay.

J Hazard Mater 2021 Mar 3;406:124596. Epub 2020 Dec 3.

College of Life Sciences, Nanjing Normal University, Nanjing 210023, China. Electronic address:

Microcystin-LR (MC-LR) is a high-toxic biohazard that pollutes ecological environment and agroproducts. In this study, a newly recombined genetically engineered antibody (AV-MV) with higher thermal stability and binding activity was designed by chain shuffling and based on our previously obtained anti-MC-LR scFv and nanobody. Based on AV-MV template, a capacity of 8.99 × 10 CFU/mL of phage display AV-MV mutagenesis library was constructed by site-directed mutagenesis in MV-CDR3 region, and then used for ultrasensitive mutants screening. Afterwards, a total of five positive AV-MV mutants were isolated from the mutagenesis library, and their binding activity was higher than AV-MV for MC-LR. The AV-MV mutant 3 was cloned into pET-25b vector for soluble expression, and the concentration of target protein expressed in culture system was 43.5 mg/L. An indirect competitive enzyme-linked immunosorbent assay (IC-ELISA) was established based on purified AV-MV mutant 3 protein, and it showed ultrasensitive binding activity for MC-LR with the detection limit of 0.0075 μg/L, which was far below the maximum residue limit standard of 1.0 μg/L in drinking water proposed by World Health Organization. The established IC-ELISA shows good accuracy, repeatability, stability and applicability for MC-LR spiked samples, and it is promising for MC-LR ultrasensitive monitoring.
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http://dx.doi.org/10.1016/j.jhazmat.2020.124596DOI Listing
March 2021

Curcuma's extraction attenuates propranolol-induced psoriasis like in mice by inhibition of keratin, proliferating cell nuclear antigen and toll-like receptor expression.

Pak J Pharm Sci 2020 May;33(3):1033-1048

Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, Chengdu, China.

Curcuma was the dried rhizomes of Curcuma kwangsiensis S.G. Lee et C.F. Liang (Chinese name: e zhu), have been used in China for thousands of years. There are some reports have shown that curcumin, the major component of curcuma, has a good curative effect on psoriasis, but the mechanism is still unknown, so the present study was designed to investigate the effect of curcuma's extraction on psoriasis-like mouse, and to explore the mechanisms of therapy. First, we observed that curcuma's extractions effect on mitosis of mouse vaginal epithelial cells; then making psoriasis like model and measuring the score of skin damage on days 7 and 14; finally, we observed the expression of immune factors (CK14, CK16, CK17, PCNA, TLR-2, TLR-4, TLR-9) in propranolol induced psoriasis like rats. Curcuma's extraction prohibited the mitosis of mouse vaginal epithelial cells; curcuma's extractions have a significantly efficacy and dose dependent inhibition on imiquimod induced psoriasis like rats; and the expression of immune factors (CK14, CK16, CK17, PCNA, TLR-2, TLR-4, TLR-9) was decreasing in the curcuma's extraction treated groups compared with normal groups. Our research proved that curcuma's extractions have a significantly efficacy on psoriasis like rats, thus, curcuma's extractions can be a potential novel treatment for psoriasis. Furthermore, the expression of immune factors was decreasing after treatment with curcuma's extraction suggest us cytokines has strong relation with the mechanism of therapy for psoriasis. Our results contribute towards validation of curcuma in the treatment of psoriasis and other joint disorders.
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May 2020

The role of non-coding RNA network in atherosclerosis.

Life Sci 2021 Jan 13;265:118756. Epub 2020 Nov 13.

Institute of Heart and Vessel Diseases, The Second Affiliated Hospital of Dalian Medical University, Dalian Medical University, Dalian 116023, People's Republic of China; Department of Cardiology, The Second Affiliated Hospital of Dalian Medical University, Dalian 116023, People's Republic of China. Electronic address:

Atherosclerosis is the primary culprit of cardiovascular and cerebrovascular diseases. Also, atherogenesis and the development of atherosclerosis involve endothelial cells, monocytes/macrophages, smooth myocytes, and others. Increasingly, studies have found that non-coding RNA (ncRNA) which can regulate apoptosis, pyroptosis, autophagy, proliferation, and monocyte migration participates in atherogenesis and progress of atherosclerosis by the above. The ncRNA networks may be essential in regulating the complicated process of atherosclerosis. Accordingly, this review delves into the regulatory roles of ncRNA, which were introduced previously. The answer above is particularly crucial to explain further the regulatory mechanism of ncRNA in cardiovascular disorders. Furthermore, we discuss the possibility and related research of ncRNAs as a biomarker and therapeutic target for the prevention, diagnosis, and treatment of atherosclerosis.
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http://dx.doi.org/10.1016/j.lfs.2020.118756DOI Listing
January 2021

iTRAQ‑based proteomic analysis of the interaction of A549 human lung epithelial cells with Aspergillus fumigatus conidia.

Mol Med Rep 2020 Dec 11;22(6):4601-4610. Epub 2020 Oct 11.

Department of Pathogenobiology, Jilin University Mycology Research Center, College of Basic Medical Sciences, Jilin University, Changchun, Jilin 130021, P.R. China.

Severe invasive aspergillosis infection occurs when human immune function is impaired. The interaction between Aspergillus fumigatus (A. fumigatus) conidia and type II lung epithelial cells serves an important role in disease progression. The present study compared the proteomes of A549 human lung epithelial cells with and without A. fumigatus infection. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) and protein interaction analyses were performed, and differential protein expression was verified by western blotting and reverse transcription‑quantitative PCR (RT‑qPCR). In addition, the RNA interference method, an internalization assay and ELISA were performed. Isobaric tags for relative and absolute quantification analysis detected a total of 1,582 proteins, from which 111 proteins with differential expression were obtained (fold change >1.5 or <0.75). Among them, 18 proteins were upregulated and 93 proteins were downregulated in A549 cells challenged with A. fumigatus. GO and KEGG analyses revealed that the altered proteins were mainly involved in biological functions, such as cell metabolism, synthesis, the cellular stress response, metabolic pathways and pyruvate metabolism. N‑myc downstream‑regulated gene 1 (NDRG1) expression was upregulated 1.88‑fold, while CD44 expression was downregulated 0.47‑fold following A. fumigatus infection. The expression levels of specific proteins were verified by western blotting and RT‑qPCR. The internalization efficiency was affected by NDRG1 gene silencing. The secretion of IL‑6 and IL‑8 was affected when CD44 was inhibited. These results indicated that A. fumigatus affects lung epithelial cell metabolism and biological synthetic functions. A number of novel molecules, including NDRG1 and CD44, were found to be related to A. fumigatus infection.
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http://dx.doi.org/10.3892/mmr.2020.11582DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7646843PMC
December 2020

Systematic analysis to identify transcriptome-wide dysregulation of Alzheimer's disease in genes and isoforms.

Hum Genet 2021 Apr 2;140(4):609-623. Epub 2020 Nov 2.

Department of Medical Research Center, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, 107 Yan Jiang West Road, Guangzhou, 500001, People's Republic of China.

Alzheimer's disease (AD) is one of the most common neurodegeneration diseases caused by multiple factors. The mechanistic insight of AD remains limited. To disclose molecular mechanisms of AD, many studies have been proposed from transcriptome analyses. However, no analysis across multiple levels of transcription has been conducted to discover co-expression networks of AD. We performed gene-level and isoform-level analyses of RNA sequencing (RNA-seq) data from 544 brain tissues of AD patients, mild cognitive impaired (MCI) patients, and healthy controls. Gene and isoform levels of co-expression modules were constructed by RNA-seq data. The associations of modules with AD were evaluated by integrating cognitive scores of patients, Genome-wide association studies (GWAS), alternative splicing analysis, and dementia-related genes expressed in brain tissues. Totally, 29 co-expression modules were found with expressions significantly correlated with the cognitive scores. Among them, two isoform modules were enriched with AD-associated SNPs and genes whose mRNA splicing displayed significant alteration in relation to AD disease. These two modules were further found enriched with dementia-related genes expressed in four brain regions of 125 AD patients. Analyzing expressions of these two modules revealed expressions of 39 isoforms (corresponding to 35 genes) significantly correlated with cognitive scores of AD patients, in which 38 isoforms were significantly up-regulated in AD patients comparing to controls, and 33 isoforms (corresponding to 29 genes) were not reported as AD-related previously. Employing the co-expression modules and the drug-induced gene expression data from Connectivity Map (CMAP), 12 drugs were predicted as significant in restoring the gene expression of AD patients towards health, which include nine drugs reported for relieving AD. In comparison, four of the top 12 significant drugs were known for relieving AD if the drug prediction was performed by the genes expressed significantly different in AD and healthy controls. Analysis of multiple levels of the transcriptomic organization is useful in suggesting AD-related co-expression networks and discovering drugs.
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http://dx.doi.org/10.1007/s00439-020-02230-7DOI Listing
April 2021

A Design Principle for Polar Assemblies with C -Sym Bowl-Shaped π-Conjugated Molecules.

Angew Chem Int Ed Engl 2021 Feb 10;60(6):3261-3267. Epub 2020 Dec 10.

RIKEN Center for Emergent Matter Science, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan.

Polar materials attract wide research interest due to their unique properties, such as ferroelectricity and the bulk photovoltaic effect (BPVE), which are not accessible with nonpolar materials. However, in general, rationally designing polar materials is difficult because nonpolar materials are more favorable in terms of dipole-dipole interactions. Here, we report a rational strategy to form polar assemblies with bowl-shaped π-conjugated molecules and a molecular design principle for this strategy. We synthesized and thoroughly characterized 12 single crystals with the help of various theoretical calculations. Furthermore, we demonstrated that it can be possible to predict whether polar assemblies become more favorable or not by estimating their lattice energies. We believe that this study contributes to the development of organic polar materials and their related studies.
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http://dx.doi.org/10.1002/anie.202013333DOI Listing
February 2021

Interferon-α inducible protein 6 (IFI6) confers protection against ionizing radiation in skin cells.

J Dermatol Sci 2020 Nov 24;100(2):139-147. Epub 2020 Sep 24.

Second Affiliated Hospital of Chengdu Medical College, China National Nuclear Corporation 416 Hospital, Chengdu, China; West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu, China. Electronic address:

Background: Radiation-induced skin injury is one of the main adverse effects and a dose-limiting factor of radiotherapy without feasible treatment. The underlying mechanism of this disease is still limited.

Objective: To investigate the potential molecular pathways and mechanisms of radiation-induced skin injury.

Methods: mRNA expression profiles were determined by Affymetrix Human HTA2.0 microarray.IFI6 overexpression and knockdown were mediated by lentivirus. The functional changes of skin cells were measured by flow cytometry, ROS probe and Edu probe. Protein distribution was detected by immunofluorescence experiment, and IFI6-interacting proteins were detected by immunoprecipitation (IP) combined with mass spectrometry. The global gene changes in IFI6-overexpressed skin cells after irradiation were detected by RNA-seq.

Results: mRNA expression profiling showed 50 upregulated and 13 down regulated genes and interferon alpha inducible protein 6 (IFI6) was top upregulated. Overexpression of IFI6 promoted cell proliferation and reduced cell apoptosis as well as ROS production following radiation, and conversely, increased the radiosensitivity of HaCaT and human skin fibroblast (WS1). IFI6 was translocated into nucleus in irradiated skin cells and the interacting relationship with mitochondrial single-stranded DNA-binding protein 1 (SSBP1), which could enhance the transcriptional activity of heat shock transcription factor 1 (HSF1).IFI6 augmented HSF1 activity following radiation in HaCaT and WS1 cells. RNA-seq analysis showed IFI6 modulated virus infection and cellular response to stress pathways, which may help to further explore how IFI6 regulate the transcriptional activity of HSF1.

Conclusion: This study reveals that IFI6 is induced by ionizing radiation and confers radioprotection in skin cells.
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http://dx.doi.org/10.1016/j.jdermsci.2020.09.003DOI Listing
November 2020

Bioglass enhances the production of exosomes and improves their capability of promoting vascularization.

Bioact Mater 2021 Mar 30;6(3):823-835. Epub 2020 Sep 30.

Shanghai Jiao Tong University Affiliated Sixth People's Hospital, School of Biomedical Engineering, Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai, 200030, China.

Recently, exosomes have been extensively applied in tissue regeneration. However, their practical applications are severely restricted by the limited exosome secretion capability of cells. Therefore, developing strategies to enhance the production of exosomes and improve their biological function attracts great interest. Studies have shown that biomaterials can significantly enhance the paracrine effects of cells and exosomes are the main signal carriers of intercellular paracrine communication, thus biomaterials are considered to affect the exosome secretion of cells and their biological function. In this study, a widely recognized biomaterial, 45S5 Bioglass® (BG), is used to create a mild and cell-friendly microenvironment for mesenchymal stem cells (MSCs) with its ion products. Results showed that BG ion products can significantly improve exosome production of MSCs by upregulating the expression of neutral sphingomyelinase-2 (nSMase2) and Rab27a which enhanced the nSMases and Rab GTPases pathways, respectively. Besides, microRNA analysis indicates that BG ion products can modulate the cargoes of MSCs-derived exosomes by decreasing microRNA-342-5p level while increasing microRNA-1290 level. Subsequently, the function of exosomes is modified as their capabilities of promoting the vascularization of endothelial cells and facilitating the intradermal angiogenesis are enhanced. Taken together, BG ion products are confirmed to enhance exosome production and simultaneously improve exosome function, suggesting a feasible approach to improve the practical application of exosomes in regenerative medicine.
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http://dx.doi.org/10.1016/j.bioactmat.2020.09.011DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7530219PMC
March 2021

Distinct RNA demethylation pathways catalyzed by nonheme iron ALKBH5 and FTO enzymes enable regulation of formaldehyde release rates.

Proc Natl Acad Sci U S A 2020 10 28;117(41):25284-25292. Epub 2020 Sep 28.

Department of Chemistry, University of California, Berkeley, CA 94720;

The AlkB family of nonheme Fe(II)/2-oxoglutarate-dependent oxygenases are essential regulators of RNA epigenetics by serving as erasers of one-carbon marks on RNA with release of formaldehyde (FA). Two major human AlkB family members, FTO and ALKBH5, both act as oxidative demethylases of 6-methyladenosine (m6A) but furnish different major products, 6-hydroxymethyladenosine (hm6A) and adenosine (A), respectively. Here we identify foundational mechanistic differences between FTO and ALKBH5 that promote these distinct biochemical outcomes. In contrast to FTO, which follows a traditional oxidative -demethylation pathway to catalyze conversion of m6A to hm6A with subsequent slow release of A and FA, we find that ALKBH5 catalyzes a direct m6A-to-A transformation with rapid FA release. We identify a catalytic R130/K132/Y139 triad within ALKBH5 that facilitates release of FA via an unprecedented covalent-based demethylation mechanism with direct detection of a covalent intermediate. Importantly, a K132Q mutant furnishes an ALKBH5 enzyme with an m6A demethylation profile that resembles that of FTO, establishing the importance of this residue in the proposed covalent mechanism. Finally, we show that ALKBH5 is an endogenous source of FA in the cell by activity-based sensing of FA fluxes perturbed via ALKBH5 knockdown. This work provides a fundamental biochemical rationale for nonredundant roles of these RNA demethylases beyond different substrate preferences and cellular localization, where m6A demethylation by ALKBH5 versus FTO results in release of FA, an endogenous one-carbon unit but potential genotoxin, at different rates in living systems.
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http://dx.doi.org/10.1073/pnas.2007349117DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7568336PMC
October 2020

Intestinal Barrier Breakdown and Mucosal Microbiota Disturbance in Neuromyelitis Optical Spectrum Disorders.

Front Immunol 2020 2;11:2101. Epub 2020 Sep 2.

Multiple Sclerosis Center, Department of Neurology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.

Background And Purpose: The mechanism underlying the pathology of neuromyelitis optica spectrum disorders (NMOSD) remains unclear even though antibodies to the water channel protein aquaporin-4 (AQP4) on astrocytes play important roles. Our previous study showed that dysbiosis occurred in the fecal microbiota of NMOSD patients. In this study, we further investigated whether the intestinal barrier and mucosal flora balance are also interrupted in NMOSD patients.

Methods: Sigmoid mucosal biopsies were collected by endoscopy from six patients with NMOSD and compared with samples from five healthy control (HC) individuals. These samples were processed for electron microscopy and immunohistochemistry to investigate changes in ultrastructure and in the number and size of intestinal inflammatory cells. Changes in mucosal flora were also analyzed by high-throughput 16S ribosomal RNA gene amplicon sequencing.

Results: The results from bacterial rRNA gene sequencing showed that bacterial diversity was decreased, but and were abundant in the colonic mucosa specimens of NMOSD patients compared to the HC individuals. The intercellular space between epithelia of the colonic mucosa was wider in NMOSD patients compared to the HC subjects ( < 0.01), and the expression of tight junction proteins [occludin, claudin-1 and zonula occludens-1 (ZO-1)] in NMOSD patients significantly decreased compared to that in the HC subjects. We also found numerous activated macrophages with many inclusions within the cytoplasm, mast cells with many particles in their cytoplasm, and enlarged plasma cells with rich developed rough endoplasmic reticulum in the lamina propria of the mucosa of the patients with NMOSD. Quantitative analysis showed that the percentages of small CD38+ and CD138+ cells (plasma cells) were lower, but the percentage of larger plasma cells was higher in NMOSD patients.

Conclusion: The present study demonstrated that the intestinal barrier was disrupted in the patients with NMOSD, accompanied by dysbiosis and inflammatory activation of the gut. The mucosal microbiota imbalance and inflammatory responses might allow pathogens to cross the damaged intestinal barrier and participate in pathological process in NMOSD. However, further study on the pathological mechanism of NMOSD underlying gut dysbiosis is warranted in the future.
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http://dx.doi.org/10.3389/fimmu.2020.02101DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7492665PMC
September 2020

Effects of forced swimming stress on expression and phosphorylation of PI3K/Akt signal pathway in pancreas of type 2 diabetic rats.

Ann Transl Med 2020 Aug;8(16):1006

Department of Geriatrics, Shenzhen People's Hospital, The First Affiliated Hospital of Southern University of Science and Technology, The Second Clinical Medical College of Jinan University, Shenzhen, China.

Background: To study the effects of forced swimming stress on blood glucose and insulin levels and the expression and phosphorylation of pancreatic PI3K/Akt signal pathway in type 2 diabetic rats.

Methods: Thirty adult SD rats (8-week-old, male) were randomly divided into three groups: control group, diabetic model group, and diabetic model stress group. The diabetic model group was established by feeding with the high-fat and high-glucose diet for four weeks, and then the rats were injected with low-dose streptozotocin (STZ, 25 mg/kg, once a day for 2 days). The rats in the diabetic model group were subjected to forced swimming stress for seven days, which was a diabetic model stress group. Twenty-four hours after the last forced swimming stress, the rats fasted, and blood samples were collected to detect blood glucose, insulin, triglycerides (TGs), free fatty acids (FFAs), high- and low-density lipoprotein cholesterol (HDLC and LDLC) levels. The western blot was applied to detect the expression of PI3K, Akt, p-Akt, and mTOR in the pancreas of rats in each group.

Results: Blood glucose and insulin levels in the diabetic model stress group were significantly lower than those in the diabetic model group, and the protein levels of PI3K, p-AKT, and mTOR in the pancreas of the diabetic model stress group were significantly higher than those of the diabetic model group.

Conclusions: One-week swimming stress can decrease the blood glucose level and improve insulin indexes in type 2 diabetic rats and increase the expression and phosphorylation of pancreatic PI3K/Akt signal pathway proteins.
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http://dx.doi.org/10.21037/atm-20-5304DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7475502PMC
August 2020

Establishment of a low-tumorigenic MDCK cell line and study of differential molecular networks.

Biologicals 2020 Nov 11;68:112-121. Epub 2020 Sep 11.

College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, 730030, PR China. Electronic address:

Influenza is an acute respiratory infection caused by the influenza virus, and vaccination against influenza is considered the best way to prevent the onset and spread. MDCK (Madin-Darby canine kidney) cells are typically used to isolate the influenza virus, however, their high tumorigenicity is the main controversy in the production of influenza vaccines. Here, MDCK-C09 and MDCK-C35 monoclonal cell lines were established, which were proven to be low in tumorigenicity. RNA-seq of MDCK-C09, MDCK-C35, and MDCK-W73 cells was performed to investigate the putative tumorigenicity mechanisms. Tumor-related molecular interaction analysis of the differentially expressed genes indicates that hub genes, such as CUL3 and EGFR, may play essential roles in tumorigenicity differences between MDCK-C (MDCK-C09 and MDCK-C35) and MDCK-W (MDCK-W73) cells. Moreover, the analysis of cell proliferation regulation-associated molecular interaction shows that downregulated JUN and MYC, for instance, mediate increased proliferation of these cells. The present study provides a new low-tumorigenic MDCK cell line and describes the potential molecular mechanism for the low tumorigenicity and high proliferation rate.
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http://dx.doi.org/10.1016/j.biologicals.2020.07.003DOI Listing
November 2020

Potentially inappropriate medications in Chinese older adults: a comparison of two updated Beers criteria.

Int J Clin Pharm 2021 Feb 13;43(1):229-235. Epub 2020 Sep 13.

Department of Pharmacy, Drum Tower Hospital Affiliated to the Medical School of Nanjing University, No. 321 of Zhongshan Road, Gulou District, 210008, Nanjing, China.

Background Beers criteria have been into the mainstay to characterize the potentially inappropriate medication since its first publication, but the recent version, Beers 2019, is yet to be validated by clinical studies nationally. Objective To identify the prevalence and the predictors of potentially inappropriate medications in hospitalized geriatric patients based on the Beers 2019 and 2015 criteria. Setting Nanjing Drum Tower Hospital, a 3000-bed tertiary care teaching hospital in China. Method We conducted a cross-sectional study from July 1, 2018 to December 31, 2018. Data from all hospitalized patients aged ≥ 65 years were collected from the hospital database. Inappropriate prescriptions were identified using the Beers 2019 criteria and the Beers 2015 criteria. Main outcome measure Prevalence Ratio (PR) and predictors of potentially inappropriate medications. Results The prevalence of inappropriate prescriptions based on the Beers 2019 criteria was 64.80%. This result was slightly higher than that of the Beers 2015 criteria (64.31%). The most commonly encountered inappropriate prescriptions identified using the two criteria were proton-pump inhibitors. The kappa coefficient was 0.826 (p < 0.001) indicating a strong coherence between the two criteria. The most important factor associated with inappropriate medications use was the number of prescribed drugs (PR 5.17, 95% CI 2.89-8.43; PR 4.58, 95% CI 1.93-7.25). Conclusion This study showed a high prevalence of potentially inappropriate medication in the Chinese geriatric population, which was associated with the number of prescribed drugs. The predictors identified in this research might help pharmacists to detect high-risk drugs and intervene in time.
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http://dx.doi.org/10.1007/s11096-020-01139-5DOI Listing
February 2021

A Network Pharmacology Analysis of the Active Components of the Traditional Chinese Medicine Zuojinwan in Patients with Gastric Cancer.

Med Sci Monit 2020 Aug 31;26:e923327. Epub 2020 Aug 31.

Department of Integrated Traditional Chinese and Western Medicine, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China (mainland).

BACKGROUND Zuojinwan (ZJW) is a traditional Chinese prescription normally used for gastritis. Several studies indicated that it could fight against gastric cancer. This study was designed to determine the potential pharmacological mechanism of ZJW in the treatment of gastric cancer. MATERIAL AND METHODS Bioactive compounds and potential targets of ZJW and related genes of gastric cancer were retrieved from public databases. Pharmacological mechanisms including crucial ingredients, potential targets, and signaling pathways were determined using protein-protein interaction (PPI) and Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. Virtual docking was performed to validate the findings. RESULTS Network analysis identified 47 active ZJW compounds, and 48 potential ZJW target genes linked to gastric cancer. Quercetin, beta-sitosterol, isorhamnetin, wogonin, and baicalein were identified as potential candidate agents. Our PPI analysis results combined with previously published results indicated that matrix metalloproteinases family members MMP9, MMP1, and MMP3 may play key roles in the anti-gastric cancer effect of ZJW. Molecular docking analysis showed that these crucial targets had good affinity for the representative components in ZJW. GO and KEGG enrichment analysis showed that ZJW target genes functioned in multiple pathways for treating gastric cancer, including interleukin-17 signaling and platinum drug resistance. CONCLUSIONS Our results illuminate the active ingredients, associated targets, biological processes, and signaling pathways of ZJW in the treatment of gastric cancer. This study enhances our understanding of the potential effects of ZJW in gastric cancer and demonstrates a feasible method for discovering potential drugs from Chinese medicinal formulas.
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http://dx.doi.org/10.12659/MSM.923327DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7482508PMC
August 2020

A GC-MS-Based Metabolomics Investigation of the Protective Effect of Liu-Wei-Di-Huang-Wan in Type 2 Diabetes Mellitus Mice.

Int J Anal Chem 2020 13;2020:1306439. Epub 2020 Aug 13.

Hunan Academy of Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410013, China.

Materials And Methods: MKR mice were used for the development of diabetes with high-fat diet feeding. These mice were further injected with streptozocin (STZ) to aggravate kidney failure. Fasting blood glucose (FBG) and urinary albumin-to-creatinine ratio (ACR values) were determined to validate the successful establishment of diabetic models with desired kidney dysfunction. Metabolomics approach coupled with gas chromatography-mass spectrometry (GC-MS) and random forest (RF) algorithm was proposed to discover the metabolic differences among model group and control group as well as to examine the therapeutic efficacy of traditional Chinese medicine, Liu-Wei-Di-Huang-Wan (LWDHW), in diabetes and associated kidney failure.

Results: Some metabolites such as 3-hydroxybutyric acid, citric acid, hexadecanoic acid, and octadecanoic acid showed significant differences between the control group and model group. Treatment with LWDHW resulted in a significant decrease in FBG and ACR values. These results suggested that LWDHW could have beneficial effects in diabetes-associated renal failure.
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http://dx.doi.org/10.1155/2020/1306439DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7443003PMC
August 2020

Gamma-irradiation degraded sulfated polysaccharide from a new red algal strain Sookwawon 104 with antiproliferative activity.

Oncol Lett 2020 Oct 6;20(4):91. Epub 2020 Aug 6.

Zhejiang Provincial Key Laboratory for Water Environment and Marine Biological Resources Protection, College of Life and Environmental Science, Wenzhou University, Wenzhou, Zhejiang 325035, P.R. China.

Sookwawon 104 is a newly cultivated strain of red marine algae. The present study aimed to investigate the antiproliferative activity of sulfated polysaccharide extracted from Sookwawon 104 (PYSP), as well as that of its low molecular weight (Mw) derivatives. PYSP is a heterogeneous sulfated polysaccharide mainly composed of galactose, glucose and fucose. PYSP was degraded by gamma-irradiation at doses of 20 and 100 kGy to produce two derivatives, named as PYSP-20 and PYSP-100, respectively. Comparison of PYSP, PYSP-20 and PYSP-100 revealed clear differences in their molecular weight (Mw) distributions, and distinct antiproliferative activities against Hep3B, MDA-MB-231 and HeLa cancer cell lines. PYSP-20 and PYSP-100 exhibited stronger antiproliferative effects than PYSP, suggesting that the reduction in Mw may have increased the antiproliferative activity. Furthermore, the mRNA expression levels of the antitumor gene and cell cycle-associated genes , Cyclin B1 and cyclin dependent kinase 1 () were further analyzed by reverse transcription-quantitative PCR in PYSP-20 and PYSP-100-treated cancer cells. PYSP and its derivatives were shown to inhibit the proliferation of tumor cells by regulating the expression of , Cyclin B1 and . In conclusion, low-Mw polysaccharide derivatives prepared from Sookwawon 104 by gamma-irradiation exhibit significant inhibition effects on cancer cell proliferation and may be a novel source of potential anticancer therapeutic agents.
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http://dx.doi.org/10.3892/ol.2020.11952DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7439133PMC
October 2020

Ligand-Controlled Palladium-Catalyzed Chemoselective Multicomponent Reaction of Olefin-Tethered Aryl Halides, Isocyanides, and Carboxylic Acids: Diversified Synthesis of Imides.

Org Lett 2020 Sep 20;22(17):6784-6789. Epub 2020 Aug 20.

Shaanxi Key Laboratory of Chemical Additives for Industry, College of Chemistry and Chemical Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China.

The first palladium-catalyzed, ligand-controlled chemoselective synthesis of imides has been achieved. An orthogonal set of conditions has been developed for multicomponent reaction of various olefin-tethered aryl iodides, isocyanides, and carboxylic acids. Alkylimides ("cyclization on" products) via arylimidation of tethered unactivated alkenes and aryl imides ("cyclization off" products) via direct imidation of aryl iodides were obtained in good to excellent yields with good to excellent selectivity. Computational studies were performed to gain insight into the origin of the high levels of chemoselectivity observed.
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http://dx.doi.org/10.1021/acs.orglett.0c02297DOI Listing
September 2020