Publications by authors named "Dan Deng"

75 Publications

Comprehensive comparison of thirteen kinds of cytokine receptors from the endangered fish Chinese sturgeon (Acipenser sinensis).

Dev Comp Immunol 2021 Oct 24;123:104132. Epub 2021 May 24.

Sturgeon Healthy Breeding and Medicinal Value Research Center, Basic Medical College, Guizhou University of Traditional Chinese Medicine, Guiyang, 550025, China.

The interferon receptor system in teleost fish is more complex than that in mammals. In the present study, we identified 13 cytokine receptor genes (10 interferon receptor genes and 3 IL10R2-like genes) from Chinese sturgeon (Acipenser sinensis) using RNA-sequencing. Sequence analysis indicated that these receptors had conserved domains, including signal peptides, FNⅢ, and transmembrane domains. Phylogenetic analysis suggested that they belonged to the cytokine receptor family. In the present study, we named them IFNAR1-like (CRFB5a, CRFB5b), IFNAR2-like (CRFB3a, CRFB3b), IFNGR1-like (IFNGR1), IFNGR2-like (CRFB6a, CRFB6b/IFNGR2-1, CRFB6c/IFNGR2-2, CRFB6d/IFNGR2-3, CRFB6e/IFNGR2-4) and IL10R2-like (CRFB4a, CRFB4b, CRFB4c), respectively. Constitutive expression analysis revealed that these receptor genes had potential functions in immune and non-immune tissue compartments. After stimulating with Poly (I:C), the expression fold changes of CRFB3a, CRFB4a, CRFB4b, CRFB5b, and CRFB6e/IFNGR2-4 in Chinese sturgeon were higher than those of other receptor genes, which revealed that these five genes had important functions in the immune process to resist virus invasion in the host. After stimulating with IFN gamma, the expression fold changes of CRFB3a, CRFB4a, and CRFB6b/IFNGR2-1 were higher than those other receptor genes. Based on other teleost fish interferon receptor models, we speculated that IFNAR1-like (CRFB5a, CRFB5b) and IFNAR2-like (CRFB3a, CRFB3b), comprised Chinese sturgeon type Ⅰ IFN receptors; and IFNGR1-like (IFNGR1) and IFNGR2-like (CRFB6/IFNGR2) comprised Chinese sturgeon type Ⅱ IFN receptors.
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http://dx.doi.org/10.1016/j.dci.2021.104132DOI Listing
October 2021

Association between gastroesophageal reflux disease and vocal fold polyps.

Medicine (Baltimore) 2021 May;100(20):e25787

Department of Gastroenterology, Second Affiliated Hospital, Chongqing Medical University, Yuzhong District, Chongqing, China.

Abstract: The aim of this study is to explore the relationship between gastroesophageal reflux disease (GERD) and vocal fold polyps (VFPs).This is a Case-Control study and was performed with the help of The Second Affiliated Hospital of Chongqing Medical University.Twenty-seven patients with VFP and 20 controls without VFP were recruited between May and October 2018. All the subjects underwent a saliva pepsin test, completed the GerdQ questionnaire and 24-hour multichannel intraluminal impedance with pH (24-h MII-pH) monitoring. Twenty-five resected VFP specimens were examined with immunohistochemical (IHC) and double immunofluorescence (IF) staining.The incidence of GERD in the VFP group was significantly higher than that in the control group (P = .003). Patients with VFP had significantly higher GerdQ scores, pepsin concentrations, and pepsin-positive rates (P < .05). Moreover, the number of proximal and upright reflux events was significantly higher in the VFP group (P < .05). The pepsin concentration in saliva showed a significant positive correlation with the pepsin levels in tissues (r2 = 0.50, P = .011). Pepsin and TGF-β1-positive cells were colocalized with CD45RO-positive cells. IHC staining showed that the majority of VFP patients had a positive expression of pepsin (20/25, 80%) and pepsin-positive cells were found in both the squamous epithelium and mesenchymal tissues. IHC staining of TGF-β1 in VFP revealed findings similar to those of pepsin staining.GERD is an important risk factor for VFP. Pepsin may promote the aggregation of immune cells, increase the local cytokines, and promote inflammatory reaction, suggesting a potential new pathogenesis for VFP. The saliva pepsin test is a reliable method for GERD diagnosis.
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http://dx.doi.org/10.1097/MD.0000000000025787DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8137056PMC
May 2021

Nuclear Factor-Y in Mouse Pancreatic β-Cells Plays a Crucial Role in Glucose Homeostasis by Regulating β-Cell Mass and Insulin Secretion.

Diabetes 2021 May 12. Epub 2021 May 12.

Molecular Medicine Research Center and National Clinical Research Center for Geriatrics, West China Hospital, and State Key Laboratory of Biotherapy, Sichuan University, Chengdu, China

Pancreatic β-cell mass and insulin secretion are determined by the dynamic change of transcription factor expression levels in response to altered metabolic demand. Nuclear factor-Y (NF-Y) is an evolutionarily conserved transcription factor playing critical roles in multiple cellular processes. However, the physiological role of NF-Y in pancreatic β-cells is poorly understood. The current study was undertaken in a conditional knockout of specifically in pancreatic β-cells ( βKO) to define the essential physiological role of NF-Y in β-cells. βKO mice exhibited glucose intolerance without changes in insulin sensitivity. Reduced β-cell proliferation resulting in decreased β-cell mass was observed in these mice, which was associated with disturbed actin cytoskeleton. NF-Y-deficient β-cells also exhibited impaired insulin secretion with a reduced Ca influx in response to glucose, which was associated with an inefficient glucose uptake into β-cells due to a decreased expression of GLUT2 and a reduction in ATP production resulting from the disruption of mitochondrial integrity. This study is the first to show that NF-Y is critical for pancreatic islet homeostasis and function through regulation in β-cell proliferation, glucose uptake into β-cells, and mitochondrial energy metabolism. Modulating NF-Y expression in β-cells may therefore offer an attractive approach for therapeutic intervention.
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http://dx.doi.org/10.2337/db20-1238DOI Listing
May 2021

Impact of China's zero mark-up drug policy on drug cost of NCDs' outpatients: an interrupted time series analysis.

BMC Health Serv Res 2021 Apr 29;21(1):404. Epub 2021 Apr 29.

School of Public Health and Management, Chongqing Medical University, Chongqing, China.

Background: China proposed the Zero Markup Drug Policy (ZMDP), which popularized in tertiary hospitals across the country in 2017, to control drug expenditures' rapid growth further and reduce the public's medical burden. This study aims to evaluate the impact of ZMDP on the drug cost of chronic disease outpatients in the tertiary hospital in Chongqing.

Methods: We collected and described the drug-cost data for outpatients with chronic diseases in a Chongqing's tertiary hospital from 2015 to 2019. The instantaneous and long-term changes of the outpatient volume and average drug cost after the ZMDP were evaluated using interrupted time series (ITS). We also analyzed the policy's impact under the stratification of gender, age, and basic medical insurance types.

Results: A total of 350,848 outpatients were collected from January 2015 to February 2019. After the ZMDP, the outpatient volume for diabetes, hypertension, and coronary heart disease (CHD) all showed a downward trend, with a decrease of 53.04 (P = 0.012), 142.19 (P < 0.01) and 12.16 (P < 0.001) per month. Simultaneously, the average drug cost decreased by 4.44 yuan (P = 0.029), 5.87 yuan (P < 0.001) and 10.23 yuan (P = 0.036) per month, respectively. By gender, the average drug cost of diabetes in males had the most considerable instantaneous change, reducing by 51.21 yuan (P = 0.017); the decline of CHD in women is the most obvious, with an average monthly decrease of 12.51 yuan (P < 0.001). By age, the instantaneous change of CHD was the greatest for those older than 65 years old, with a decrease of 102.61 yuan (P = 0.030). CHD in 46-65 years old showed the most significant reduction, with an average monthly decline of 11.70 yuan (P < 0.01). BMIUE's hypertension had the most considerable instantaneous change, which decreased 59.63 yuan (P = 0.010). BMIUE's CHD showed the most apparent downward trend, with an average monthly decrease of 10.02 yuan (P = 0.010).

Conclusion: The ITS analysis is an effective method of health policy evaluation. The implementation of the ZMDP can reduce the drug cost for chronic disease outpatients in the tertiary hospital and their economic burden. Follow-up policies still require targeted price adjustments in the health service system to adjust the drug cost-effectively.
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http://dx.doi.org/10.1186/s12913-021-06414-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8086109PMC
April 2021

A multi-tissue multi-omics analysis reveals distinct kineztics in entrainment of diurnal transcriptomes by inverted feeding.

iScience 2021 Apr 19;24(4):102335. Epub 2021 Mar 19.

Department of Cardiology and the Center for Circadian Metabolism and Cardiovascular Disease, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, 400038, China.

Time of eating synchronizes circadian rhythms of metabolism and physiology. Inverted feeding can uncouple peripheral circadian clocks from the central clock located in the suprachiasmatic nucleus. However, system-wide changes of circadian metabolism and physiology entrained to inverted feeding in peripheral tissues remain largely unexplored. Here, we performed a 24-h global profiling of transcripts and metabolites in mouse peripheral tissues to study the transition kinetics during inverted feeding, and revealed distinct kinetics in phase entrainment of diurnal transcriptomes by inverted feeding, which graded from fat tissue (near-completely entrained), liver, kidney, to heart. Phase kinetics of tissue clocks tracked with those of transcriptomes and were gated by light-related cues. Integrated analysis of transcripts and metabolites demonstrated that fatty acid oxidation entrained completely to inverted feeding in heart despite the slow kinetics/resistance of the heart clock to entrainment by feeding. This multi-omics resource defines circadian signatures of inverted feeding in peripheral tissues (www.CircaMetDB.org.cn).
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http://dx.doi.org/10.1016/j.isci.2021.102335DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8050734PMC
April 2021

The predictive value of brain natriuretic peptide or N-terminal pro-brain natriuretic peptide for weaning outcome in mechanical ventilation patients: Evidence from SROC.

J Renin Angiotensin Aldosterone Syst 2021 Jan-Dec;22(1):1470320321999497

Department of Critical Care Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, P.R. China.

Objective: Mechanical ventilation is an important treatment for critically ill patients. Physicians generally perform a spontaneous breathing trial (SBT) to determine whether the patients can be weaned from mechanical ventilation, but almost 17% of the patients who pass the SBT still require respiratory support. Cardiac dysfunction is an important cause of weaning failure. The use of brain natriuretic peptide or N-terminal pro-BNP is a simple method to assess cardiac function. We performed a systematic review of investigations of brain natriuretic peptide or N-terminal pro-BNP as predictors of weaning from mechanical ventilation.

Data Sources: PubMed (1950 to December 2020), Cochrane, and Embase (1974 to December 2020), and some Chinese databases for additional articles (China Biology Medicine (CBM), China Science and Technology Journal Database (CSTJ), and Wanfang Data and China National Knowledge Infrastructure (CNKI)).

Study Selection: We systematically searched observation studies investigating the predictive value of brain natriuretic peptide or N-terminal pro-brain natriuretic peptide in weaning outcome of patients with mechanical ventilation.

Data Extraction: Two independent reviewers extracted data. The differences are resolved through consultation.

Data Synthesis: We included 18 articles with 1416 patients and extracted six index tests with pooled sensitivity and specificity for each index test. For the BNP change rate predicting weaning success, the pooled sensitivity was 89% (83%-94%) and the pooled specificity was 82% (72%-89%) with the highest pooled AUC of 0.9511.

Conclusions: The brain natriuretic peptide change rate is a reliable predictor of weaning outcome from mechanical ventilation.
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http://dx.doi.org/10.1177/1470320321999497DOI Listing
March 2021

Roles of the H19/microRNA‑675 axis in the proliferation and epithelial‑mesenchymal transition of human cutaneous squamous cell carcinoma cells.

Oncol Rep 2021 04 2;45(4). Epub 2021 Mar 2.

Department of Dermatology, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200092, P.R. China.

The long non‑coding RNA (lncRNA) H19 and microRNA(miR)‑675 were reported to serve an important role in the tumorigenesis and metastasis of numerous cancer types by promoting the epithelial‑mesenchymal transition (EMT) process; however, the underlying mechanisms of action of H19 and miR‑675 in cutaneous squamous cell carcinoma (cSCC) remain unknown. The mRNA expression levels of H19 and miR‑675 were analyzed using reverse transcription‑quantitative PCR, and Cell Counting Kit‑8, wound healing and Transwell assays were performed to analyze the cell proliferation, migration and invasion of cSCC cells, respectively. The levels of cell apoptosis were also determined using a TUNEL assay. Protein expression levels of p53 and marker proteins related to the EMT process were analyzed using western blotting. In addition, a dual luciferase reporter assay was performed to determine the interactions between H19, miR‑675 and p53. The results of the present study revealed that the expression levels of H19 and miR‑675 were upregulated in cSCC tissues and cSCC cell lines. The knockdown of H19 or miR‑675 expression inhibited cell proliferation, migration and invasion, but induced cell apoptosis. In addition, the expression levels of EMT‑related markers were also downregulated. The overexpression of H19 upregulated the expression levels of its predicted target, miR‑675, which subsequently promoted the EMT process and downregulated the expression levels of p53. Conversely, the genetic silencing of H19 or miR‑675 inhibited proliferation and invasion in SCL1 and A431 cSCC cell lines. In conclusion, the findings of the present study provided novel insight into the potential role of H19 and miR‑675 in the development, metastasis and progression of cSCC, which may help the development of treatments for cSCC.
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http://dx.doi.org/10.3892/or.2021.7990DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7905556PMC
April 2021

Pharmacokinetics and Pharmacodynamics of Esomeprazole/Sodium Bicarbonate Immediate-Release Capsules in Healthy Chinese Volunteers: A Cross-Over, Randomized Controlled Trial.

Adv Ther 2021 03 11;38(3):1660-1676. Epub 2021 Feb 11.

Clinical Pharmacology Centre, Beijing Anzhen Hospital, Capital Medical University, Beijing, China.

Introduction: Esomeprazole delayed release tablets (ESO) are one of the most effective treatments for acid-related disorders. The purpose of this study is to compare the safety, pharmacokinetics (PK) and pharmacodynamics (PD) of an immediate-release capsule formulation containing esomeprazole 20 mg and sodium bicarbonate 1100 mg (IR-ESO) compared to those of the esomeprazole delayed release tablet 20 mg (ESO). In addition, the impact of CYP2C19 gene polymorphisms on PK and PD was evaluated.

Methods: A single-center, open-label, randomized, 2-treatment, 2-sequence, and 2-period crossover study was conducted in 40 healthy Chinese subjects. Subjects received either IR-ESO or ESO for 5 days. After single- and multiple-dosing administration, blood samples were collected for PK analysis, and intragastric pH was assessed by 24-h pH monitoring. The CYP2C19 gene polymorphisms were analyzed by Sanger sequencing.

Results: The geometric mean ratios (90% confidence interval) [GMR (95%CI)] of IR-ESO/ESO for AUC [single dose: 103.60% (96.58%, 111.14%), multiple doses: 101.65% (97.88%, 105.57%)] were within the range of 80.00-125.00%. The AUC showed an increasing trend between CYP2C19 extensive metabolizer (EM), intermediate metabolizer (IM), and poor metabolizer (PM) after single-dose and multiple-dose administration (p < 0.05). The GMR (95%CI) of IR-ESO/ESO for 24-h integrated gastric acidity from baseline [single dose: 101.07% (96.56%, 105.78%), multiple doses: 101.24% (97.74%, 104.86%)] were within the range of 80.00-125.00%. The percentage changes in 24-h integrated gastric acidity from baseline was significant difference between EM, IM, and PM after single-dose IR-ESO and ESO (p < 0.05). Drugs were all well tolerated, and there were no significant differences in adverse events between IR-ESO and ESO.

Conclusion: This study showed that IR-ESO can inhibit the secretion of gastric acid rapidly and continuously, and that the PK and PD of IR-ESO are affected by CYP2C19 genotypes. The GMR (95% CI) of IR-ESO/ESO for AUC and the percentage changes in 24-h integrated gastric acidity from baseline were all within the range of 80.00-125.00%.

Trial Registration: Chinese Clinical Trial Registry: ChiCTR1900024935.
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http://dx.doi.org/10.1007/s12325-021-01644-7DOI Listing
March 2021

Evaluate the performance of four artificial intelligence-aided diagnostic systems in identifying and measuring four types of pulmonary nodules.

J Appl Clin Med Phys 2021 Jan 24;22(1):318-326. Epub 2020 Dec 24.

School of Public Health and Management, Chongqing Medical University, Chongqing, China.

Purpose: This study aims to evaluate the performance of four artificial intelligence-aided diagnostic systems in identifying and measuring four types of pulmonary nodules.

Methods: Four types of nodules were implanted in a commercial lung phantom. The phantom was scanned with multislice spiral computed tomography, after which four systems (A, B, C, D) were used to identify the nodules and measure their volumes.

Results: The relative volume error (RVE) of system A was the lowest for all nodules, except for small ground glass nodules (SGGNs). System C had the smallest RVE for SGGNs, -0.13 (-0.56, 0.00). In the Bland-Altman test, only systems A and C passed the consistency test, P = 0.40. In terms of precision, the miss rate (MR) of system C was 0.00% for small solid nodules (SSNs), ground glass nodules (GGNs), and solid nodules (SNs) but 4.17% for SGGNs. The comparable system D MRs for SGGNs, SSNs, and GGNs were 71.30%, 25.93%, and 47.22%, respectively, the highest among all the systems. Receiver operating characteristic curve analysis indicated that system A had the best performance in recognizing SSNs and GGNs, with areas under the curve of 0.91 and 0.68. System C had the best performance for SGGNs (AUC = 0.91).

Conclusion: Among four types nodules, SGGNs are the most difficult to recognize, indicating the need to improve higher accuracy and precision of artificial systems. System A most accurately measured nodule volume. System C was most precise in recognizing all four types of nodules, especially SGGN.
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http://dx.doi.org/10.1002/acm2.13142DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7856495PMC
January 2021

Porous Aromatic Framework Nanosheets Anchored with Lewis Pairs for Efficient and Recyclable Heterogeneous Catalysis.

Adv Sci (Weinh) 2020 Nov 1;7(22):2000067. Epub 2020 Oct 1.

Key Laboratory of Polyoxometalate Science of Ministry of Education Northeast Normal University Renmin Avenue Changchun 130024 China.

Lewis pairs (LPs) with outstanding performance for nonmetal-mediated catalysis reactions have high fundamental interest and remarkable application prospects. However, their solubility characteristics lead to instability and deactivation upon recycling. Here, the layered porous aromatic framework (PAF-6), featuring two kinds of Lewis base sites (N and N), is exfoliated into few-layer nanosheets to form the LP entity with the Lewis acid. After comparison with various porous networks and verification by density functional theory (DFT) calculations, the N atom in the specific spatial environment is determined to preferably coordinate with the electron-deficient boron compound in a sterically hindered pattern. LP-bare porous product displays high catalytic activity for the hydrogenation of both olefin and imine compounds, and demonstrates ≈100% activity after 10 successful cycles in hydrogenation reactions. Considering the natural advantage of porous organic frameworks to construct LP groups opens up novel prospects for preparing other nonmetallic heterogeneous catalysts for efficient and recyclable catalysis.
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http://dx.doi.org/10.1002/advs.202000067DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7675047PMC
November 2020

Correlation Between the Clinical Severity, Bacterial Load, and Inflammatory Reaction in Children with Mycoplasma Pneumoniae Pneumonia.

Curr Med Sci 2020 Oct 29;40(5):822-828. Epub 2020 Oct 29.

Department of Pulmonology, Children's Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing, 400014, China.

Given the lack of defining features in the clinical manifestations and radiographic findings for children with mycoplasma pneumoniae pneumonia (MPP), quantitative polymerase chain reaction (qPCR) has become a useful diagnostic method. This study was performed to explore the relationship between the qPCR findings, clinical symptoms, and inflammatory markers in children with MPP. Four hundred children with MPP have been enrolled in this retrospective analysis. All clinical and analytical information, including mycoplasma pneumoniae (MP) PCR results, has been collected. Based on the PCR results, the patients were divided into groups with load values (copy number) < 10 (54 cases), ≥10 and <10 (71 cases), ≥10 and <10 (112 cases), ≥10 and ≤10 (114 cases), and >10 (49 cases). The clinical features (including symptoms and signs) and inflammatory indicators were compared among the groups. The incidence of high fever (above 39°C), thermal peak during the entire hospitalization period, fever duration, days of hospitalization, and plasma lactate dehydrogenase (LDH) levels were statistically correlated with the MP PCR load value in children with MPP. The analysis of relevance degree showed the correlative order as a thermal peak of hospitalization > duration of fever > period of hospitalization > LDH value > C-reactive protein value. The host immune response was significantly greater in the complication group than in the non-complication group.
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http://dx.doi.org/10.1007/s11596-020-2261-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7595045PMC
October 2020

Kutane Metastasen eines dreifachen primären extramammären Morbus Paget.

J Dtsch Dermatol Ges 2020 Oct;18(10):1169-1172

Department of Dermatology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.

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http://dx.doi.org/10.1111/ddg.14189_gDOI Listing
October 2020

Enzyme-Inspired Assembly: Incorporating Multivariate Interactions to Optimize the Host-Guest Configuration for High-Speed Enantioselective Catalysis.

ACS Appl Mater Interfaces 2020 Oct 7;12(42):47966-47974. Epub 2020 Oct 7.

Key Laboratory of Polyoxometalate Science of Ministry of Education, Northeast Normal University, Changchun 130024, China.

To achieve a rapid asymmetry conversion, the substrate objects suffer from accelerated kinetic velocity and random rotation at the cost of selectivity. Inspired by natural enzymes, optimizing the host-guest configuration will realize the high-performance enantioselective conversion of chemical reactions. Herein, multivariate binding interactions were introduced into the 1D channel of a chiral catalyst to simulate the enzymatic action. An imidazolium group was used to electrophilically activate the C═O unit of a ketone substrate, and the counterion binds the hydrogen donor isopropanol. This binding effect around the catalytic center produces strong stereo-induction, resulting in high conversion (99.5% yield) and enantioselectivity (99.5% ee) for the asymmetric hydrogenation of biomass-derived acetophenone. In addition, the turnover frequency of the resulting catalyst (5160 h TOF) is more than 58 times that of a homogeneous Ru-TsDPEN catalyst (88 h TOF) under the same condition, which corresponds to the best performance reported till date among all existing catalysts for the considered reaction.
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http://dx.doi.org/10.1021/acsami.0c13802DOI Listing
October 2020

Cutaneous metastases from triple primary extramammary Paget's disease.

J Dtsch Dermatol Ges 2020 10 27;18(10):1169-1172. Epub 2020 Jul 27.

Department of Dermatology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.

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http://dx.doi.org/10.1111/ddg.14189DOI Listing
October 2020

Physiological response and oxidative transformation of 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) by a Chlorella isolate.

Sci Total Environ 2020 Nov 14;744:140869. Epub 2020 Jul 14.

Department of Chemistry, City University of Hong Kong, Tat Chee Avenue, Kowloon Tong, Kowloon, Hong Kong, China; State Key Laboratory in Marine Pollution, City University of Hong Kong, Tat Chee Avenue, Kowloon Tong, Kowloon, Hong Kong, China. Electronic address:

Polybrominated diphenyl ethers (PBDEs) are ubiquitous, toxic and persistent pollutants in environments. Microalgae frequent exposed to these pollutants may possess defense mechanisms against their toxicity and have the ability to metabolize them, thus are important in bioremediation. This study investigated the mechanism of a Chlorella isolate to degrade BDE-47, a common PBDE congener, and its subcellular responses to BDE-47 stress. Results showed that 86-98% of the spiked BDE-47 was removed by Chlorella via adsorption, uptake and metabolism. BDE-47 was metabolized through debromination, hydroxylation and methoxylation. The oxidative transformation to hydroxylated products was the initial and main metabolic process. BDE-47 induced the production of hydrogen peroxide (HO) in cell wall, plasma membrane and chloroplast of Chlorella, and such increase was regulated by nicotinamide adenine dinucleotide phosphate oxidase and HO-producing peroxidases (PODs). The activity of HO-consuming PODs and the content of glutathione were also significantly enhanced to detoxify the oxidative stress.
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http://dx.doi.org/10.1016/j.scitotenv.2020.140869DOI Listing
November 2020

Effects of genetic variations in Acads gene on the risk of chronic obstructive pulmonary disease.

IUBMB Life 2020 09 27;72(9):1986-1996. Epub 2020 Jun 27.

Molecular Medicine Research Center and National Clinical Research Center for Geriatrics, West China Hospital, State Key Laboratory of Biotherapy, Sichuan University, Chengdu, China.

Short-chain acyl-CoA dehydrogenase (SCAD), encoded by the Acads gene, functions in the mitochondrial β-oxidation of saturated short-chain fatty acids. SCAD deficiency results in mitochondrial dysfunction, which is one underlying biological mechanism of chronic obstructive pulmonary disease (COPD) pathogenesis. In this case-control study, we aimed to examine the effects of Acads gene polymorphisms on the susceptibility to COPD. A total of 16 tagging single-nucleotide polymorphisms (SNPs) in Acads gene region was identified and genotyped in 646 unrelated ethnic Chinese Han individuals including 279 patients with COPD and 367 healthy controls, their allelic and genotypic associations with COPD were determined by different genetic models. Furthermore, we estimated the linkage disequilibrium and haplotypes from these tested variants and determined the effects of haplotypes on the risk of COPD. The allelic and genotypic frequencies of SNPs rs2239686 and rs487915 in Acads gene were significantly different between COPD patients and controls, no statistically significant results were observed for other SNPs. Minor alleles A of rs2239686 and T of rs487915 were associated with a decreased pulmonary function and an increased COPD risk in a dominant manner. Functional analysis indicated that the risk allele A of rs2239686 could increase Acads expressions and the intracellular reactive oxygen species content. Haplotype analysis revealed that the haplotypes CTCCT in block 2 (rs3794216-rs3794215-rs34491494-rs558314-rs7312316) as well as GC in block 3 (rs2239686-rs487915) were protective against COPD, while haplotypes CTCGC in block 2 and AT in block 3 exhibited significant associations with the increased susceptibility to COPD. Our results suggest that Acads gene could potentially be a risk factor of COPD and thus its genetic variants might be as genetic biomarkers to predict the COPD susceptibility.
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http://dx.doi.org/10.1002/iub.2336DOI Listing
September 2020

Next-generation sequencing through multigene panel testing for the diagnosis of hereditary epidermolysis bullosa in Chinese population.

Clin Genet 2020 08 21;98(2):179-184. Epub 2020 Jun 21.

Department of Dermatology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.

Epidermolysis bullosa (EB) is a heritable blistering disorder. We performed a next-generation sequencing-based multigene panel test and successfully predicted 100% of the EB types, including, 36 EB simplex (EBS), 13 junctional EB (JEB), 86 dystrophic EB (DEB), and 3 Kindler EB. Chinese JEB and recessive DEB (RDEB) patients have relatively mild phenotypes; for severe type separately accounts for 45.5% and 23.8%, respectively. We identified 96 novel and 49 recurrent pathogenic variants in 11 genes, although we failed to detect the second mutation in one JEB and five RDEB patients. We identified one novel p.E475K mosaic mutation in the clinically normal mother of one out of 13 EBS patients with KRT5 mutations, one recurrent p.G2034R mosaic mutation, and one novel p.G2043R mosaic mutation in the clinically normal relatives of two out of 19 dominant DEB patients. This study shows that next-generation technology could be an effective tool in diagnosing EB.
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http://dx.doi.org/10.1111/cge.13791DOI Listing
August 2020

Modulation of Donor Alkyl Terminal Chains with the Shifting Branching Point Leads to the Optimized Morphology and Efficient All-Small-Molecule Organic Solar Cells.

ACS Appl Mater Interfaces 2020 Jun 19;12(22):25100-25107. Epub 2020 May 19.

CAS Key Laboratory of Nanosystem and Hierarchical Fabrication, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing 100190, China.

Terminal group modification is one of the most influential factors for small-molecular donors compared with their polymer counterparts, resulting in an opportunity to optimize the morphology of all-small-molecule organic solar cells (ASM-OSCs). In this article, we report three novel small-molecular donors with branching points at the 1-, 2-, and 3-positions in alkyl terminal chains, called , , and , respectively. Using IDIC-4Cl as the acceptor, the subtle branching position shift achieves a dramatic disparity in photovoltaic parameters, as indicated by the short circuit current () changing from 4.9 to 20.1 to 14.2 mA cm and the fill factor varying from 33.9 to 71.3 to 67.0% for , , and , respectively. The best device performance of 12.40% is obtained by the :IDIC-4Cl system, which not only ranks among the top values reported to date but also exhibits low energy loss in systems that use IDIC as acceptors. The notable device performance based on is attributed to the optimized phase morphology caused by the strong molecular crystallinity and suitable intermolecular interaction with IDIC-4Cl. These results demonstrate that suitably tuning the branching position of terminal groups could promote the high performance of ASM-OSCs.
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http://dx.doi.org/10.1021/acsami.0c03977DOI Listing
June 2020

Porous Organic Frameworks Featured by Distinct Confining Fields for the Selective Hydrogenation of Biomass-Derived Ketones.

Adv Mater 2020 Jun 22;32(22):e1908243. Epub 2020 Apr 22.

Key Laboratory of Polyoxometalate Science of Ministry of Education, Northeast Normal University, Renmin Avenue, Changchun, 130024, China.

The asymmetric hydrogenation of biomass-derived molecules for the preparation of single enantiomer compounds is an effective method to reduce the rapid consumption of fossil resources. Porous organic frameworks (POFs) with pure organic surfaces may provide unusual confinement effects for organic substrates in chiral catalysis. Here, a series of POF catalysts are designed with chiral active centers decorated into sharply defined one-dimensional channels with diameters in the range of 1.2-2.9 nm. Due to the synergistic effect originating from the conjugated inner wall, the POF material (aperture size 2.4 nm) concentrates over 90% of aromatic species into the porous architecture, and its affinity is one or two orders of magnitude higher than those of classical porous solids. As determined by PBE+D3 calculation, the phenyl fragment reveals strong π-π interaction for steric hindrance around the metal active site to achieve stronger asymmetric induction. Therefore, this POF catalyst achieves high conversion (>99% yield) and enantioselectivity (>99% ee) for various substrates. The advantages of using the POF platform as a chiral catalyst can provide new perspectives on POF-based solid-state host-guest chemistry and asymmetric heterogeneous catalysis.
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http://dx.doi.org/10.1002/adma.201908243DOI Listing
June 2020

Deep Learning-Based Secure MIMO Communications with Imperfect CSI for Heterogeneous Networks.

Sensors (Basel) 2020 Mar 20;20(6). Epub 2020 Mar 20.

Department of Computing and Mathematics, Manchester Metropolitan University, Manchester M15 6BH, UK.

Perfect channel state information (CSI) is required in most of the classical physical-layer security techniques, while it is difficult to obtain the ideal CSI due to the time-varying wireless fading channel. Although imperfect CSI has a great impact on the security of MIMO communications, deep learning is becoming a promising solution to handle the negative effect of imperfect CSI. In this work, we propose two types of deep learning-based secure MIMO detectors for heterogeneous networks, where the macro base station (BS) chooses the null-space eigenvectors to prevent information leakage to the femto BS. Thus, the bit error rate of the associated user is adopted as the metric to evaluate the system performance. With the help of deep convolutional neural networks (CNNs), the macro BS obtains the refined version from the imperfect CSI. Simulation results are provided to validate the proposed algorithms. The impacts of system parameters, such as the correlation factor of imperfect CSI, the normalized doppler frequency, the number of antennas is investigated in different setup scenarios. The results show that considerable performance gains can be obtained from the deep learning-based detectors compared with the classical maximum likelihood algorithm.
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http://dx.doi.org/10.3390/s20061730DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7146336PMC
March 2020

Synthesis of [email protected] Core-Shell Nanoparticles with Tunable Shell Thickness and Their Degradation Mechanism in Aqueous Solutions.

Langmuir 2020 Apr 25;36(13):3386-3392. Epub 2020 Mar 25.

Division of Materials Science and Engineering, Faculty of Engineering, Hokkaido University, Kita 13 Nishi 8, Kita-ku, Sapporo, Hokkaido 060-8628, Japan.

[email protected] core-shell nanoparticles (NPs) are widely used in photocatalysts, sensors, and optical applications owing to their unique metal-semiconductor interface and the integration of the properties from both core and shell materials. Although many efforts have been made toward the precise synthesis of [email protected] core-shell structures, the chemical stability of [email protected] aqueous suspensions, which is of great significance in many related applications, is not mentioned in any published research. Herein we report the synthesis of [email protected] core-shell NPs with small shell thickness from 2 to 40 nm through a wet-chemistry method. The UV-vis absorption properties are found to be tunable with CuO thickness in the range of 2-40 nm. Furthermore, the chemical stability of [email protected] core-shell nanoparticle suspensions in water/ethanol mixed solvents is investigated. It is found that water/ethanol mixed solvents with a larger amount of water are more likely to deteriorate the stability of [email protected] NPs by oxidizing CuO to CuO. The results from this work may provide useful information for the preparation of [email protected] water-based suspensions that are expected to be used for SERS, photocatalyst, or photothermal applications.
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http://dx.doi.org/10.1021/acs.langmuir.0c00382DOI Listing
April 2020

Silencing MEG3 protects PC12 cells from hypoxic injury by targeting miR-21.

Authors:
Dan Deng Hui Liang

Artif Cells Nanomed Biotechnol 2020 Dec;48(1):610-619

Department of Neurology, Hainan General Hospital, Haikou, China.

Increasing number of literatures highlighted lncRNA maternally expressed gene 3 (MEG3) as an emerging target for hypoxic-ischaemic brain damage (HIBD). This study attempted to assess the role of MEG3 in a cell model of HIBD. Expression of MEG3 in PC12 cells was suppressed by siRNA-mediated transfection, after which the cells were subjected to hypoxia. Cell viability, apoptosis, migration and the expression of related proteins were assessed. Furthermore, the downstream gene of MEG3 and its downstream signalling pathways were explored. We found that, down-regulation of MEG3 prevented hypoxic injury in PC12 cells, as hypoxia induced viability loss, apoptosis and migration repression were attenuated by transfection with MEG3 siRNA. Meanwhile, MEG3 acted as a miR-21 sponge. The neuroprotective functions of MEG3 silence were flattened when miR-21 was suppressed. Moreover, the deactivation of PI3K/AKT pathway and the activation of NF-κB pathway induced by hypoxia were attenuated by MEG3 silence. As expected, the effects of MEG3 silence on these two signalling were miR-21. In conclusion, the neuroprotective effects of MEG3 silence on PC12 cells injured by hypoxia were observed in this study. Mechanistically, the neuroprotective effects of MEG3 silence on PC12 cells were sponging miR-21 and thus regulating PI3K/AKT and NF-κB pathways.HIGHLIGHTSMEG3 is highly expressed in PC12 cells following hypoxic injury;Silence of MEG3 prevents hypoxia-induced cell damage in PC12 cells;MEG3 acts as a miR-21 sponge;MEG3 sponges miR-21 to regulate PI3K/AKT and NF-κB pathways.
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http://dx.doi.org/10.1080/21691401.2020.1725533DOI Listing
December 2020

Electrospun metformin-loaded polycaprolactone/chitosan nanofibrous membranes as promoting guided bone regeneration membranes: Preparation and characterization of fibers, drug release, and osteogenic activity in vitro.

J Biomater Appl 2020 04 21;34(9):1282-1293. Epub 2020 Jan 21.

Department of Implantology, State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.

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http://dx.doi.org/10.1177/0885328220901807DOI Listing
April 2020

Epidemiological characteristics of bacillary dysentery from 2009 to 2016 and its incidence prediction model based on meteorological factors.

Environ Health Prev Med 2019 Dec 28;24(1):82. Epub 2019 Dec 28.

School of Public Health and Management, Research Center for Medicine and Social Development, Innovation Center for Social Risk Governance in Health, Chongqing Medical University, Chongqing, 400016, China.

Background: This study aimed to analyse the epidemiological characteristics of bacillary dysentery (BD) caused by Shigella in Chongqing, China, and to establish incidence prediction models based on the correlation between meteorological factors and BD, thus providing a scientific basis for the prevention and control of BD.

Methods: In this study, descriptive methods were employed to investigate the epidemiological distribution of BD. The Boruta algorithm was used to estimate the correlation between meteorological factors and BD incidence. The genetic algorithm (GA) combined with support vector regression (SVR) was used to establish the prediction models for BD incidence.

Results: In total, 68,855 cases of BD were included. The incidence declined from 36.312/100,000 to 23.613/100,000, with an obvious seasonal peak from May to October. Males were more predisposed to the infection than females (the ratio was 1.118:1). Children < 5 years old comprised the highest incidence (295.892/100,000) among all age categories, and pre-education children comprised the highest proportion (34,658 cases, 50.335%) among all occupational categories. Eight important meteorological factors, including the highest temperature, average temperature, average air pressure, precipitation and sunshine, were correlated with the monthly incidence of BD. The obtained mean absolute percent error (MAPE), mean squared error (MSE) and squared correlation coefficient (R) of GA_SVR_MONTH values were 0.087, 0.101 and 0.922, respectively.

Conclusion: From 2009 to 2016, BD incidence in Chongqing was still high, especially in the main urban areas and among the male and pre-education children populations. Eight meteorological factors, including temperature, air pressure, precipitation and sunshine, were the most important correlative feature sets of BD incidence. Moreover, BD incidence prediction models based on meteorological factors had better prediction accuracies. The findings in this study could provide a panorama of BD in Chongqing and offer a useful approach for predicting the incidence of infectious disease. Furthermore, this information could be used to improve current interventions and public health planning.
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http://dx.doi.org/10.1186/s12199-019-0829-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6935186PMC
December 2019

All-small-molecule organic solar cells with over 14% efficiency by optimizing hierarchical morphologies.

Nat Commun 2019 11 26;10(1):5393. Epub 2019 Nov 26.

CAS key laboratory of nanosystem and hierarchical fabrication, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, 100190, Beijing, China.

The high efficiency all-small-molecule organic solar cells (OSCs) normally require optimized morphology in their bulk heterojunction active layers. Herein, a small-molecule donor is designed and synthesized, and single-crystal structural analyses reveal its explicit molecular planarity and compact intermolecular packing. A promising narrow bandgap small-molecule with absorption edge of more than 930 nm along with our home-designed small molecule is selected as electron acceptors. To the best of our knowledge, the binary all-small-molecule OSCs achieve the highest efficiency of 14.34% by optimizing their hierarchical morphologies, in which the donor or acceptor rich domains with size up to ca. 70 nm, and the donor crystals of tens of nanometers, together with the donor-acceptor blending, are proved coexisting in the hierarchical large domain. All-small-molecule photovoltaic system shows its promising for high performance OSCs, and our study is likely to lead to insights in relations between bulk heterojunction structure and photovoltaic performance.
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http://dx.doi.org/10.1038/s41467-019-13292-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6879588PMC
November 2019

Valproic acid promotes the epithelial-to-mesenchymal transition of breast cancer cells through stabilization of Snail and transcriptional upregulation of Zeb1.

Eur J Pharmacol 2019 Dec 19;865:172745. Epub 2019 Oct 19.

Department of Breast Surgery, The Central Hospital of Yongzhou, Yongzhou, 425000, China.

Histone deacetylases (HDACs) can regulate cancer progression and its inhibitors (HDACIs) have been widely used for cancer therapy. Valproic acid (VPA, 2-propylpentanoic acid) can inhibit the class I HDAC and suppress the malignancy of solid cancers. Our present study revealed that 1 mM VPA, which has no effect on cell proliferation, can significantly increase the migration and induce epithelial to mesenchymal transition (EMT) like properties of breast cancer cells. Further, VPA increased the expression of EMT-transcription factors (EMT-TFs) Snail and Zeb1. Knockdown of Snail and Zeb1 can attenuate VPA induced cell migration and EMT. Mechanistically, VPA increased the protein stability of Snail via suppression its phosphorylation at Ser 11. As to Zeb1, VPA can increase its promoter activity and transcription via a HDAC2 dependent manner. Over expression of HDAC2 can block VPA induced expression of Zeb1. Collectively, our data revealed that VPA can trigger the EMT of breast cancer cells via upregulation of Snail and Zeb1. It indicated that more attention should be paid to the effects of VPA on the clinical therapy of breast cancer.
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http://dx.doi.org/10.1016/j.ejphar.2019.172745DOI Listing
December 2019

A Molecular Coordination Template Strategy for Designing Selective Porous Aromatic Framework Materials for Uranyl Capture.

ACS Cent Sci 2019 Aug 19;5(8):1432-1439. Epub 2019 Jul 19.

Key Laboratory of Polyoxometalate Science of Ministry of Education, Northeast Normal University, Changchun 130024, P. R. China.

Uranium capture from seawater could solve increasing energy demand and enable a much needed relaxing from fossil fuels. Low concentration (∼3 ppb), competing cations (especially vanadium) and pH-dependent speciation prohibit highly efficient uranium uptake. Despite intensive research, selective extraction of uranyl ions over vanadyl units remains a tremendous challenge. Here, we adopted a molecular coordination template strategy to design a uranyl-specific bis-salicylaldoxime entity and decorated it into a highly porous aromatic framework (PAF-1) by programmable assembly. The superstructure (MISS-PAF-1) gives a strong affinity that removes 99.97% of uranium in 120 min. Notably, it binds to the uranyl ion at least 100 times more selectively than 14 different cations tested, including the vanadyl ion, in simulated seawater at ambient pH. Real seawater samples collected from the Bohai Sea achieve 5.79 mg g of uranium capacity over 56 days without PAF degradation, exceeding a 4-fold higher amount than commercial adsorbents.
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http://dx.doi.org/10.1021/acscentsci.9b00494DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6716130PMC
August 2019

Electrogenerated alkaline hydrogen peroxide for rice straw pretreatment to enhance enzymatic hydrolysis.

Bioresour Technol 2019 Nov 28;292:122077. Epub 2019 Aug 28.

Key Laboratory of Green Chemical Process of Ministry of Education, Key Laboratory of Novel Reactor and Green Chemical Technology of Hubei Province, School of Chemical Engineering and Pharmacy, Wuhan Institute of Technology, Wuhan 430205, China.

In this work, alkaline hydrogen peroxide (AHP) solution with 1 wt% HO was electrogenerated by oxygen reduction with a current efficiency of 75.2% in a home-made gas diffusion electrode-based electrochemical cell and used for rice straw pretreatment (0.1 g HO/g rice straw, 10% (w/v) biomass loading, 55 °C, 2 h). Results showed that the AHP pretreatment removed 97.56% of the initial lignin, 85.75% of the initial hemicellulose, and only 0.56% of the initial cellulose, and the specific surface area and porosity of the AHP pretreated rice straw (AHP-RS) were greatly increased. Saccharification results showed that after 48 h of enzymatic hydrolysis AHP-RS achieved a 3.2-fold increase in reducing sugar concentration compared to the untreated rice straw (5.81 and 1.81 g L), highlighting the potential use of this AHP solution for lignocellulose pretreatment.
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http://dx.doi.org/10.1016/j.biortech.2019.122077DOI Listing
November 2019

Enhanced osteogenesis and angiogenesis by PCL/chitosan/Sr-doped calcium phosphate electrospun nanocomposite membrane for guided bone regeneration.

J Biomater Sci Polym Ed 2019 11 13;30(16):1505-1522. Epub 2019 Aug 13.

Department of Implantology, State Key Laboratory of Oral Diseases, West China College of Stomatology, Sichuan University , Chengdu , China.

Membranes play pivotal role in guided bone regeneration (GBR) technique for reconstruction alveolar bone. GBR membrane that is able to stimulate both osteogenic and angiogenic differentiation of cells may be more effective in clinic practice. Herein, we fabricated the Sr-doped calcium phosphate/polycaprolactone/chitosan (Sr-CaP/PCL/CS) nanohybrid fibrous membrane by incorporating 20 wt% bioactive Sr-CaP nanoparticles into PCL/CS matrix via one-step electrospinning method, in order to endow the membrane with stimulation of osteogenesis and angiogenesis. The physicochemical properties, mechanical properties, Sr release behavior, and the membrane stimulate bone mesenchymal stem cell (BMSCs) differentiation were evaluated in comparison with PCL/CS and CaP/PCL/CS membranes. The SEM images revealed that the nanocomposite membrane mimicked the extracellular matrix structure. The release curve presented a 28-day long continuous release of Sr and concentration which was certified in an optimal range for positive biological effects at each timepoint. The in vitro cell culture experiments certified that the Sr-CaP/PCL/CS membrane enjoyed excellent biocompatibility and remarkably promoted rat bone mesenchymal stem cell (BMSCs) adhesion and proliferation. In terms of osteogenic differentiation, BMSCs seeded on the Sr-CaP/PCL/CS membrane showed a higher ALP activity level and a better matrix mineralization. What's more, the synergism of the Sr and CaP from the Sr-CaP/PCL/CS membrane enhanced BMSCs angiogenic differentiation, herein resulting in the largest VEGF secretion amount. Consequently, the Sr-CaP/PCL/CS nanohybrid electrospun membrane has promising applications in GBR.
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http://dx.doi.org/10.1080/09205063.2019.1646628DOI Listing
November 2019

Excessive physical activity duration may be a risk factor for hypertension in young and middle-aged populations.

Medicine (Baltimore) 2019 May;98(18):e15378

Department of Cardiology, The First Affiliated Hospital of Chongqing Medical University.

Physical inactivity is known to cause many health problems globally each year. However, evidence regarding the interaction between excessive physical activity (PA) and blood pressure in young and middle-aged populations is limited.A multistage, stratified, random cluster sampling design was adopted to recruit representative samples. Participants were asked to complete a questionnaire and undergo physical examinations. Associations between prehypertension, hypertension and PA durations were examined by multivariable logistic regression.Overall, 8206 subjects (4110 men, 50.1%) aged 15 to 45 years were enrolled. The prevalence rates of prehypertension and hypertension were 45.7% and 5.0%, respectively. Among the 1913 participants who performed moderate-intensity PA for more than 700 min/wk, 118 had hypertension (6.2%) and 845 had prehypertension (44.2%). Among the 1003 participants who performed vigorous-intensity PA for more than 450 min/wk, 82 had hypertension (8.2%) and 479 had prehypertension (47.8%).Long-term and sustained PA may increase the risk for hypertension in young and middle-aged subjects. An appropriate recommendation of PA duration should be encouraged in this cohort.
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http://dx.doi.org/10.1097/MD.0000000000015378DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6504303PMC
May 2019