Publications by authors named "Dan Chen"

1,043 Publications

  • Page 1 of 1

Cerebral aneurysm image segmentation based on multi-modal convolutional neural network.

Comput Methods Programs Biomed 2021 Jul 17;208:106285. Epub 2021 Jul 17.

Department of Neurosurgery, The Third People's Hospital of Hefei, Hefei Third Clinical College of Anhui Medical University, Hefei 230022, China. Electronic address:

Background And Objective: Accurate segmentation of cerebral aneurysms in computed tomography angiography (CTA) can provide an essential reference for diagnosis and treatment. This study aimed to evaluate a more helpful image segmentation method for cerebral aneurysms.

Methods: Firstly, the original CTA images were filtered by Gaussian and Laplace, and both the processed image and original image constitute multi-modal images as input. Then, through multiple parallel convolution neural networks to multi-modal image segmentation. Eventually, all of the segmentation results were fused by linear regression to extract cerebral aneurysm and adjacent vessels.

Results: The cerebral aneurysm and adjacent vessels were extracted correctly. When the threshold value is about 0.95, the overall performance of the segmentation effect is the best. The dice, accuracy, and recall rate were different in various combinations of the three extraction methods.

Conclusion: Multi-modal convolutional neural network can improve the segmentation accuracy by multi-modal processing of the original brain CTA image.
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http://dx.doi.org/10.1016/j.cmpb.2021.106285DOI Listing
July 2021

[Co-delivery of Pirarubicin and Vinorelbine by Micelles for the Treatment of Breast Cancer].

Sichuan Da Xue Xue Bao Yi Xue Ban 2021 Jul;52(4):612-618

Key Laboratory of Drug Targeting and Delivery System of the Ministry of Education, West China School of Pharmacy, Sichuan University, Chengdu 610041, China.

Objective: To develop a pirarubicin (THP) and vinorelbine (VRL) codelivery nano-micellar system (T+V-CS micelles) of pirarubicin (THP) and vinorelbine (VRL) by using chondroitin sulfate-cholesterol polymers (CS-Chol) and DSPE-mPEG and to evaluate the therapeutic efficacy of the codelivery nano-micelles in breast cancer treatment.

Methods: T+V-CS micelles were prepared by ultrasonic-dialysis method, and the physicochemical characterization were evaluated using multiple technological means. The anti-tumor efficacy of T+V-CS micelles was evaluated by MTT assay and cell cycle arrest analysis. Evaluation of the therapeutic effect of T+V-CS micelles was carried out on xenograft 4T1 murine breast cancer bearing BALB/c mice model.

Results: T+V-CS micelles displayed a nearly spherical shape when observed through transmission electron microscope. The particle size and polydispersity indexes (PDI) of T+V-CS micelles was (155.5±4.5) nm and 0.170±0.003 respectively, while the Zeta potential was (-23.0±0.9) mV. Meanwhile, T+V-CS micelles demonstrated high encapsulation efficiency of (81.87±2.56)% for THP and (87.54±2.82)% for VRL and a high overall drug loading efficiency of (10.20±1.20)%. and studies of the therapeutic efficacy of breast cancer showed that T+V-CS micelles had synergistic anti-tumor effect and induced increased G /M cell cycle arrest in 4T1 cells, which could significantly inhibit tumor growth and prolong survival compared with the therapeutic efficacy of micelles loaded with a single kind of drug or free drug solutions.

Conclusion: The study showed that T+V-CS micelles had excellent anti-tumor effect, offering a reference to the clinical treatment of breast cancer.
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http://dx.doi.org/10.12182/20210760105DOI Listing
July 2021

Endoplasmic reticulum stress inhibits 3D Matrigel-induced vasculogenic mimicry of breast cancer cells via TGF-β1/Smad2/3 and β-catenin signaling.

FEBS Open Bio 2021 Jul 28. Epub 2021 Jul 28.

Radiation Oncology Key Laboratory of Sichuan Province, Sichuan Cancer Hospital & Institute, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China.

Endoplasmic reticulum (ER) stress is a cellular stress condition involving disturbance in the folding capacity of the ER caused by endogenous and exogenous factors. ER stress signaling pathways affect tumor malignant growth, angiogenesis and progression, and promote the anti-tumor effects of certain drugs. However, the impact of ER stress on the vasculogenic mimicry (VM) phenotype of cancer cells has not been well addressed. VM is a phenotype which mimics vasculogenesis by forming patterned tubular networks, which are related to stemness and aggressive behaviors of cancer cells. In this study we employed tunicamycin (TM), a UPR-activating agent, to induce ER stress in aggressive triple negative MDA-MB-231 breast cancer cells, which exhibit a VM phenotype in 3D Matrigel cultures. TM-induced ER stress was able to inhibit the VM phenotype. In addition to the tumorspheroid phenotype observed upon inhibiting the VM phenotype, we observed alterations in glycosylation of integrin β1, loss of VE-cadherin and a decrease in stem cell marker Bmi-1. Further study revealed decreased activated TGF-β1, Smad2/3, Phospho-Smad2 and β-catenin. β-catenin knockdown markedly inhibited the VM phenotype and resulted in the loss of VE-cadherin. The data suggest that the activation of ER stress inhibited VM phenotype formation of breast cancer cells via both the TGF-β1/Smad2/3 and β-catenin signaling pathways. The discovery of prospective regulatory mechanisms involved in ER stress and VM in breast cancer could lead to more precisely targeted therapies that inhibit vessel formation and affect tumor progression.
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http://dx.doi.org/10.1002/2211-5463.13259DOI Listing
July 2021

Hypoxia-induced alteration of RNA modifications in the mouse testis and sperm.

Biol Reprod 2021 Jul 22. Epub 2021 Jul 22.

Laboratory Animal Center, Chongqing Medical University, Yixueyuan Road 1, Yuzhong District, Chongqing, 400016, P. R. China.

Hypobaric hypoxia as an extreme environment in a plateau may have deleterious effects on human health. Studies have indicated that rush entry into a plateau may reduce male fertility and manifest in decreased sperm counts and weakened sperm motility. RNA modifications are sensitive to environmental changes and have recently emerged as novel post-transcriptional regulators in male spermatogenesis and intergenerational epigenetic inheritance. In the present study, we generated a mouse hypoxia model simulating the environment of 5500 meters in altitude for 35 days, which led to compromised spermatogenesis, decreased sperm counts, and an increased sperm deformation rate. Using this hypoxia model, we further applied our recently developed high-throughput RNA modification quantification platform based on LC-MS/MS, which exhibited the capacity to simultaneously examine 25 types of RNA modifications. Our results revealed an altered sperm RNA modifications signature in the testis (6 types) and mature sperm (11 types) under the hypoxia model, with 4 types showing overlap (Am, Gm, m7G, and m22G). Our data first drew the signature of RNA modification profiles and comprehensively analyzed the alteration of RNA modification levels in mouse testis and sperm under a mouse hypoxia model. These data may be highly related to human conditions under a similar hypoxia environment.
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http://dx.doi.org/10.1093/biolre/ioab142DOI Listing
July 2021

The complete mitogenome of Sokolov's Dwarf Hamster () and implication of phylogenetic status.

Mitochondrial DNA B Resour 2021 6;6(8):2269-2271. Epub 2021 Jul 6.

College of Life Sciences, Sichuan Normal University, Chengdu, China.

There is still an obvious lack of information on Sokolov's Dwarf Hamster () which belongs to subfamily Cricetinae because the species is only rarely found in Gobi desert. In this study, we obtained the complete mitochondrial genome sequences of The genome is 16,292bp in length and has a base composition of 33.5% A, 30.5% T, 22.9% C, and 13.1% G. The mitogenome structure, consisting of 13 protein-coding genes, two rRNA genes (12S rRNA and 16S rRNA), 22 tRNA genes, and one control region, is similar to that of typical vertebrate mitochondrial genomes of other rodents. We restructured a Bayesian phylogenetic tree by using 12 species belonging to subfamily Cricetinae. As indicated by the phylogenetic tree, genus is polyphyletic group, and is the closest relative of . The mitochondrial genome can provide basic data for further study on the phylogenetic relationship of subfamily Cricetinae.
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http://dx.doi.org/10.1080/23802359.2021.1914212DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8266243PMC
July 2021

Current Advances of Nanocarrier Technology-Based Active Cosmetic Ingredients for Beauty Applications.

Clin Cosmet Investig Dermatol 2021 13;14:867-887. Epub 2021 Jul 13.

College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, Hubei, People's Republic of China.

Nanocarrier technology has been effectively applied to the development of drug delivery systems to overcome the limitations of traditional preparation. Its application has been extended to various pharmaceutical fields from injection preparation to oral preparation and external preparation, and now it has appeared in the field of cosmetics for beauty applications. The widespread influence of nanocarrier in the cosmetics industry is due to the fact that nanocarrier can effectively promote the percutaneous penetration and significantly increase skin retention of active components in functional cosmetics. Meanwhile, nanocarrier can effectively improve the water dispersion of insoluble active cosmetic ingredients, enhance the stability of efficacy components and achieve the codelivery of diverse cosmetics active ingredients. In this review, we summarized the current progress of nanocarrier technology in the functional cosmetics, including the types and the routes of dermal/transdermal drug delivery nanocarriers used in the functional cosmetics, the mechanism of nanocarriers promoting the percutaneous penetration of active cosmetic ingredients, the application and efficacy evaluation of different active cosmetic ingredients in nanocarriers and discussing the potential risks to human. This will provide a useful reference for the further development of nanocarriers in the field of functional cosmetics.
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http://dx.doi.org/10.2147/CCID.S313429DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8286087PMC
July 2021

Stability of trivalent human papillomavirus (types 16, 18, 58) recombinant vaccine (Escherichia coli).

Chin Med J (Engl) 2021 Jul 19. Epub 2021 Jul 19.

Quality Department of Beijing Health Guard Biotechnology Inc., BDA, Daxing District, Beijing 100176, China National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China School of Chemical and Engineering, University of Chinese Academy of Sciences, Beijing 100049, China Research and Development Department of Beijing Health Guard Biotechnology Inc., BDA, Daxing District, Beijing 100176, China.

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http://dx.doi.org/10.1097/CM9.0000000000001659DOI Listing
July 2021

Reductive potential from cathode electrode as an option for the achievement of short-cut nitrification in bioelectrochemical systems.

Bioresour Technol 2021 Jul 13;338:125553. Epub 2021 Jul 13.

Jiangsu Key Laboratory of Chemical Pollution Control and Resources Reuse, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, Jiangsu Province 210094, China.

Nitrogen removal based on short-cut nitrification (SCN) have attract more attentions, in which stable nitrite accumulation is prerequisite. In this study, different reductive potential was applied to inhibit nitrite oxidizing bacteria for achievement of SCN in aerobic cathode chamber of bioelectrochemical systems with dissolved oxygen concentration of 3.5 mg/L. The results demonstrated that the applied potential facilitated nitrite accumulation with high ammonia oxidation rates. The maximum nitrate accumulation rate of 87.61% was obtained at -800 mV. The abundance of Nitrosomonas and Thauera increased while Nitrospira abundance declined with more negative reductive potentials. The activity of nitric oxide reductase was also evidently inhibited. The above-mentioned three genera were the keystone taxa in co-occurrence network with high degree and closeness centrality. Interestingly, total nitrogen (TN) removal was enhanced simultaneously in the absence of external organic carbon. Reductive potential would be a promising approach for achieving SCN and simultaneously TN removal.
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http://dx.doi.org/10.1016/j.biortech.2021.125553DOI Listing
July 2021

The role of informal caregivers for patients with glioma: a systematic review and meta-synthesis of qualitative studies.

Ann Transl Med 2021 Jun;9(12):1020

Department of Neurosurgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, China.

Background: This study aimed to systematically review, appraise, and synthesize the current evidence on the experiences and needs encountered by informal caregiver of patients with glioma throughout the disease trajectory and to provide a set of practical implications for health professionals.

Methods: Seven English databases and four Chinese databases were searched in this systematic review and meta-analysis. Additional manual searches were completed to identify primary studies, with the language limited by English and Chinese. The Joanna Briggs Institute (JBI) Critical Appraisal Checklist for Qualitative Research was used to appraise the methodological quality of each study.

Results: The systematic review included 16 papers that yielded 71 findings and 6 categories. Finally, 2 synthesized findings were extracted: (I) role transition of caregivers for glioma patients throughout the disease trajectory; (II) support and information need by caregivers of glioma patients. Accordingly, there is a need to recognize the importance of permanent and tailored support for caregivers by providing accurate, practical, and evidence-based information.

Discussion: This is the first attempt to systematically evaluate the breadth and quality of the literature concerning the experiences of caregivers with glioma patients. The results generated from the review may shed some light on problems encountered by glioma patients and their families. A limitation of this review is that in most selected studies, the reflexivity of interviewees is not addressed, which may influence the interpretation of the findings. Moreover, the selected studies were reported in English or Chinese, therefore, caution is needed in interpreting the results.
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http://dx.doi.org/10.21037/atm-21-2761DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8267327PMC
June 2021

Application of humin-immobilized biocathode in a continuous-flow bioelectrochemical system for nitrate removal at low temperature.

Environ Res 2021 Jul 15;202:111677. Epub 2021 Jul 15.

College of Urban Construction, Nanjing Tech University, Nanjing, 211816, China. Electronic address:

Solid-phase humic substances (humin) can work as an additional electron donor to support the low temperature denitrification but the reducing capacity of its non-reduced form is limited. In this study, a continuous-flow denitrifying BES with a humin-immobilized biocathode (H-BioC) was established. Humin was expected to function as a redox mediator and be persistently reduced on the cathode to provide reducing power to a denitrifying biofilm. Results showed that the H-BioC maintained a stable denitrification capacity with low nitrite accumulation for more than 100 days at 5 °C, and the specific microbial denitrification rate and electron transfer rate were 3.97-fold and 1.75-fold higher than those of the unaltered cathode. The results of repeated cycles of humin reduction and oxidation experiments further suggested that the redox activity of humin was stable. Acidovorax was the most dominant genus in both H-BioC biofilm and unaltered cathodic biofilm, while Rhodocyclaceae (unclassified_f_) was more enriched in H-BioC biofilm. Further Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) analyses indicated that biofilm formation, electron transfer, and nitrate reduction functions were more abundant in H-BioC, suggesting a possible enhancement mechanism by humin. The results of this study raise the possibility that immobilization of solid-phase humin may be a useful strategy for electrostimulated heterotrophic denitrification in groundwater where the indigenous bacteria have poor electroactivity.
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http://dx.doi.org/10.1016/j.envres.2021.111677DOI Listing
July 2021

GPS-ZTD data assimilation and its impact on wintertime haze prediction over North China Plain using WRF 3DVAR and CMAQ modeling system.

Environ Sci Pollut Res Int 2021 Jul 17. Epub 2021 Jul 17.

State Key Laboratory of Sever Weather, Chinese Academy of Meteorological Science, Beijing, 100081, China.

Severe haze frequently hits the North China Plain (NCP), especially in winter during recent years. Meteorological factors affect aerosol formation and its optical properties, and accurate meteorological fields are imperative for accurate aerosol simulations. The impacts of Global Positioning System Zenith Total Delay (GPS-ZTD) data assimilation on meteorology and aerosol simulations were evaluated in this study using the WRF-CMAQ (the Weather Research and Forecasting model and Community Multiscale Air Quality) modelling system over the NCP during 01-31 December 2019. After bias correction, GSP-ZTD data were assimilated into the WRF model using the 3DVAR technique. Two sensitivity tests (CTR and ZTD) were conducted. The WRF model had generally acceptable performance for surface and upper air meteorological variables, PM and visibility. From the aspect of BIAS, STDE, RMSE, and R, the assimilation of ZTD data improved the underestimation of ground relative humidity (RH). The improvement was more pronounced in the first 18 forecast hours. The mean RH BIAS decreased by 8%. Surface pressure was also improved in ZTD. The influence of ZTD data assimilation on ground temperature and wind tended to be neutral. The BIAS of ZTD decreased by 3% after data assimilation while STED or RMSE increased slightly. After ZTD data assimilation, the PM underestimation decreased by 3.4% over NCP. And station mean BIAS or RMSE of PM decreased at more than 70% stations. After ZTD data assimilation, the visibility overestimation was reduced by 2.5%. And more than 81% stations over had lower visibility BIAS or RMSE. Station mean PM mass concentration increased by 1.5% in ZTD. The primary aerosol species increased by approximately 1%, and most secondary aerosol species increased by greater than 2% affected by both aerosol physical and chemical process. Although the improvement of PM seems marginal from the perspective of regional or temporal average, the contribution of ZTD data assimilation on specific pollution episodes at specific stations can be great. The improvement of PM troughs was in the range of 1-5 μg/m, while the overestimation of PM peaks was reduced by few up to dozens μg/m. This will contribute to the extreme value prediction during pollution episode.
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http://dx.doi.org/10.1007/s11356-021-15248-9DOI Listing
July 2021

[Detection of drug-resistance genes of in bronchoalveolar lavage fluid of children with refractory pneumonia].

Zhongguo Dang Dai Er Ke Za Zhi 2021 Jul;23(7):707-712

Department of General Internal Medicine, Children's Hospital Affiliated to Zhengzhou University/Henan Children's Hospital/Zhengzhou Children's Hospital, Zhengzhou 450000, China.

Objective: To study the drug resistance of (MP) in the bronchoalveolar lavage fluid (BALF) of children with refractory pneumonia (RMPP) and the distribution of drug-resistance genes, as well as the association of alleles at 2063 locus of 23SrRNA V region central ring with resistance to antimicrobial drugs.

Methods: BALF specimens were collected from 245 children with RMPP who were admitted to the Children's Hospital Affiliated to Zhengzhou University from March 2016 to December 2020. A rapid cultured drug sensitivity assay was used to detect the resistance of MP isolates to nine commonly used antimicrobial drugs. The real-time PCR was used to measure MP DNA. The direct sequencing was used to detect gene mutations in MP 23SrRNA V region central ring.

Results: Among the 245 BALF specimens, 207 tested positive for MP DNA, with a positive rate of 84.5%. The results of drug susceptibility test showed that the children with RMPP had a resistance rate of > 70% to macrolide antimicrobial drugs, with the highest resistance rate to clarithromycin, followed by roxithromycin, clindamycin, acetylspiramycin, erythromycin, and azithromycin, and these children had a resistance rate of < 5% to quinolone antimicrobial drugs. Among the 207 MP DNA-positive specimens, 41 (19.8%) had no drug-resistance gene mutations and 166 (80.2%) had drug-resistance gene mutations, among which 154 (74.4%) had an A→G mutation at 2063 locus of 23SrRNA V region central ring, 7 (3.4%) had an A→G mutation at 2064 locus, and 5 (2.4%) had mutations in both 2063 and 2064 loci. Among the 166 specimens with point mutations of the MP 23SrRNA gene, 159 (95.8%) had point mutations at 2063 locus. The A→G point mutation at 2063 locus of 23SrRNA V region central ring had a great impact on resistance to macrolide antimicrobial drugs. There was a significant difference in the distribution of alleles at 2063 locus between the children with resistance to clarithromycin, roxithromycin, clindamycin, acetylspiramycin, erythromycin, and azithromycin ( < 0.05).

Conclusions: MP in the BALF of children with RMPP has a relatively high resistance rate to macrolide antimicrobial drugs. Resistance to macrolide antimicrobial drugs is closely associated with the A→G point mutation in the 23SrRNA gene, and the point mutation at 2063 locus of 23SrRNA V region central ring may affect the drug-resistance mechanism of MP.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8292659PMC
July 2021

Quality of life among older Chinese people living with HIV/AIDS: a cross-sectional study in Hunan.

Trans R Soc Trop Med Hyg 2021 Jul 15. Epub 2021 Jul 15.

Xiang Ya School of Public Health, Central South University, Changsha, Hunan 410078, People's Republic of China.

Background: There is little research to date in the area of quality of life (QoL) among older Chinese people living with HIV/AIDS (PLWHA). This study aims to explore the QoL and its influential factors among older PLWHA in China.

Methods: Cross-sectional data of 242 adults with HIV/AIDS aged 50-84 y from Hunan were analyzed to evaluate the associations of demographic characteristics, illness-related factors, HIV-related stigma and social support with QoL. The WHO QOL-HIV BREF questionnaire was used to measure QoL on six domains (physical, psychological, independence, social relationships, environment and spirituality).

Results: A total of 242 older PLWHA completed the survey, of whom 150 (62.0%) were males. Multivariate linear regression analyses identified that females, side effects of highly active antiretroviral therapy, chronic non-communicable diseases and higher levels of perceived negative self-image were negatively associated with QoL, especially in the physical, independence and spirituality domains; whereas higher education or annual income, retired and elevated levels of subjective social support or support availability were positively associated with QoL, especially in the psychological, independence, social relationships and environment domains.

Conclusions: The study findings suggest that interventions to improve QoL should focus on reducing HIV-related stigma and side effects and on enhancing economic situations and social support.
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http://dx.doi.org/10.1093/trstmh/trab101DOI Listing
July 2021

Isoflurane impairs oogenesis through germ cell apoptosis in C. elegans.

Sci Rep 2021 Jul 14;11(1):14481. Epub 2021 Jul 14.

Geriatric Anesthesia Research Unit, Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital and Harvard Medical School, 149 13th Street, Room 4310, Charlestown, MA, 02129-2060, USA.

Anesthetic isoflurane has been reported to induce toxicity. However, the effects of isoflurane on fecundity remain largely unknown. We established a system in C. elegans to investigate the effects of isoflurane on oogenesis. Synchronized L4 stage C. elegans were treated with 7% isoflurane for 4 h. Dead cells, ROS, embryos, and unfertilized eggs laid by hermaphrodites were measured by fluorescence imaging and counting. The C. elegans with losses of ced-3, cep-1, abl-1, male C. elegans, and oxidative stress inhibitor N-acetyl-cysteine were used in the interaction studies. We found that isoflurane decreased the numbers of embryos and unfertilized eggs and increased the levels of dead cells and ROS in C. elegans. The isoflurane-induced impairment of oogenesis was associated with abl-1, ced-3, but not cep-1. N-acetyl-cysteine attenuated the isoflurane-induced impairment of oogenesis in C. elegans. Mating with male C. elegans did not attenuate the isoflurane-induced changes in oogenesis. These findings suggest that isoflurane may impair oogenesis through abl-1- and ced-3-associated, but not cep-1-associated, germ cell apoptosis and oxidative stress, pending further investigation. These studies will promote more research to determine the potential effects of anesthesia on fecundity.
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http://dx.doi.org/10.1038/s41598-021-93737-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8280124PMC
July 2021

Genetic counseling and prenatal decision for hemoglobin H disease caused by the rare α2 codon 30 (-GAG) (HBA2: c.91_93delGAG) mutation and the SEA deletion: Case series study.

Taiwan J Obstet Gynecol 2021 Jul;60(4):763-765

Prenatal Diagnosis Center, The First Women and Children's Hospital of Huizhou, Huizhou, Guangdong Province, China. Electronic address:

Objective: We report a rare mutation on the α2-globin gene, HBA2: c.91_93delGAG and its potential functions.

Case Report: We mainly described four patients with hemoglobin (Hb) H disease caused by the rare mutation and the SEA deletion but diversity in clinical presentation. Two had survived to adulthood with normal physical and mental development, except for mild anemia. However, two were children, who had more severe clinical manifestations. One child had developmental disorders of speech and language and mild growth retardation, and the other child suffered from severe hemolytic crises precipitated by infection and received blood transfusion.

Conclusion: This study is of great significance for clinicians to provide genetic counseling to couples at-risk of having offspring with Hb H disease and let them make the pregnancy decision, particularly reduce the occurrence of severe Hb H disease.
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http://dx.doi.org/10.1016/j.tjog.2021.05.031DOI Listing
July 2021

A predictive clinical-radiomics nomogram for diagnosing of axial spondyloarthritis using MRI and clinical risk factors.

Rheumatology (Oxford) 2021 Jul 10. Epub 2021 Jul 10.

Department of Rheumatology, First Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, China.

Objectives: Construct and validate a nomogram model integrating the radiomics features and the clinical risk factors to differentiating axial spondyloarthritis (axSpA) in low back pain patients undergone sacroiliac joint (SIJ)- magnetic resonance imaging (MRI).

Methods: 638 patients confirmed as axSpA (n= 424) or non-axSpA (n = 214) who were randomly divided into training (n = 447) and validation cohorts (n = 191). Optimal radiomics signatures were constructed from the 3.0T SIJ-MRI using maximum relevance-minimum redundancy (mRMR) and the least absolute shrinkage and selection operator (LASSO) algorithm in the training cohort. We also included six clinical risk predictors to build clinical model. Incorporating the independent clinical factors and Rad-score, a nomogram model was constructed by multivariable logistic regression analysis. The performance of the clinical, Rad-score, and nomogram model were evaluated by ROC analysis, calibration curve and decision curve analysis (DCA).

Results: 1316 features were extracted and reduced to 15 features to build the Rad-score. The Rad-score allowed a good discrimination in the training (AUC, 0.82; 95% CI, 0.77-0.86) and the validation cohort (AUC, 0.82; 95% CI, 0.76-0.88). The clinical-radiomics nomogram model also showed favorable discrimination in the training (AUC, 0.90; 95% CI, 0.86-0.93) and the validation cohort (AUC, 0.90; 95% CI, 0.85-0.94). Calibration curves (p > 0.05) and DCA demonstrated the nomogram was useful for axSpA diagnosis in the clinical environment.

Conclusion: The study proposed a radiomics model was able to separate axSpA and non-axSpA. The clinical-radiomics nomogram can increase the efficacy for differentiating axSpA, which might facilitate clinical decision-making process.
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http://dx.doi.org/10.1093/rheumatology/keab542DOI Listing
July 2021

Lab-on-fiber sensing system based on responsive Fabry-Perot optical resonance cavities prepared throughconstruction strategy.

Nanotechnology 2021 Jul 22;32(41). Epub 2021 Jul 22.

College of Sciences, Northeastern University, Shenyang 110819, People's Republic of China.

For decades, lab-on-fiber (LOF) sensing systems have become an emerging optical sensing platform due to the features of small size and light weight. Herein, a simple and efficientconstruction strategy was reported for the preparation of LOF sensing platform based on the integration of responsive Fabry-Perot optical resonance cavity on optical fibers. The responsive Fabry-Perot optical resonance cavity with thermal poly(N-isopropylacrylamide) polymer brush layer sandwiched by two silver layers was constructed on the end surface of the optical fiber through combiningsurface-initiated polymerization and metal film deposition techniques. Owing to the thermo-responsiveness of the intermediate layer, the as-prepared LOF sensing system shows a sensitive response towards the environmental temperature. Importantly, the as-prepared LOF sensing system also possesses excellent repeatability and rapid response rate. Together with the features of high sensitivity, excellent repeatability and rapid response rate, we believe such LOF sensing system will provide a foundation for the future applications of medical diagnosis,detection and public security.
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http://dx.doi.org/10.1088/1361-6528/ac121dDOI Listing
July 2021

Tunicamycin-induced endoplasmic reticulum stress inhibits chemoresistance of FaDu hypopharyngeal carcinoma cells in 3D collagen I cultures and in vivo.

Exp Cell Res 2021 Aug 3;405(2):112725. Epub 2021 Jul 3.

Radiation Oncology Key Laboratory of Sichuan Province, Sichuan Cancer Hospital & Institute, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China. Electronic address:

The prognosis in patients with advanced head and neck squamous cell carcinoma (HNSCC) is widely affected by the resistance to chemotherapy. As a culture scaffold, collagen I was showed to promote CSC (cancer stem cell) properties of cancer cells which could be used as in vitro models to study the chemoresistance in HNSCC. Endoplasmic reticulum (ER) stress is a cellular stress condition which could affect tumor progression and promote the anti-tumor effects of certain drugs. However, the impact of ER stress on collagen I induced CSC properties and chemoresistance of HNSCC cells has not been addressed. In this study we investigated the effects of tunicamycin (TM) induced ER stress on the stemness and sensitivity to chemotherapeutic drugs of FaDu hypopharyngeal carcinoma cells in 3D (three-dimensional) collagen I cultures and mouse xenograft models. Our study revealed that Collagen I scaffold promoted CSC properties and increased G1 population of FaDu cells in 3D cultures, accompanied by maturation of integrin β1 and enhanced activated TGF-β1 concentration. Compared to 2D (two-dimensional) cultured cells, cells in 3D Collagen I scaffold exhibited significantly increased resistance to chemotherapeutic drugs of cisplatin and paclitaxel. Further analysis revealed that TM induced ER stress preferentially attenuated chemoresistance of FaDu cells in 3D collagen I, downregulated their CSC properties and TGF-β1 concentration and resulted in deglycosylation of integrin β1. TM was further evaluated in the mouse xenograft models and showed significant tumor growth inhibition in combination with paclitaxel than either TM or paclitaxel alone. Taken together, Our findings suggest that TM-induced ER stress potentiates anticancer efficacy of FaDu cells in 3D cultures and in vivo, and highlight implications for targeting chemotherapy-resistant cancer stem cells under ER stress conditions.
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http://dx.doi.org/10.1016/j.yexcr.2021.112725DOI Listing
August 2021

Identification of Cross-Reactive CD8 T Cell Receptors with High Functional Avidity to a SARS-CoV-2 Immunodominant Epitope and Its Natural Mutant Variants.

Genes Dis 2021 Jun 29. Epub 2021 Jun 29.

Department of Immunology, College of Basic Medicine, Chongqing Medical University, 1 Yixueyuan Road, Yuzhong District, Chongqing, 400016, PR China.

Despite the growing knowledge of T cell responses in COVID-19 patients, there is a lack of detailed characterizations for T cell-antigen interactions and T cell functions. Here, with a predicted peptide library from SARS-CoV-2 S and N proteins, restricted to three of the most prominent HLA-A alleles in the Asian population, we found that specific CD8 T cell responses were identified in over 75% of COVID-19 convalescent patients (15/20). A total of 15 SARS-CoV-2 epitopes from the S and N proteins were identified, and among them, 3 dominant epitopes were further characterized. We found that an epitope from the N protein, N (KTFPPTEPK), was the most dominant epitope from our selected peptide library. Importantly, we discovered 2 N-specific T cell receptors (TCRs) with high functional avidity that were independent of the CD8 co-receptor. These TCRs exhibited complementary cross-reactivity to several presently reported N mutant variants, as to the wild-type epitope. Further, the natural functions of these TCRs in the cytotoxic immunity against SARS-CoV-2 were determined with dendritic cells (DCs) and the lung organoid model. We found that the N epitope could be normally processed and endogenously presented by these different types of antigen presenting cells, to elicit successful activation and effective cytotoxicity of CD8 T cells . Our study evidenced potential mechanisms of cellular immunity to SARS-CoV-2, and illuminated potential ways of viral clearance in COVID-19 patients. These results indicate that utilizing CD8-independent TCRs against SARS-CoV-2-associated antigens may provide functional superiority that is beneficial for the adoptive cell immunotherapies based on natural or genetically engineered T cells. Additionally, this information is highly relevant for the development of the next-generation vaccines with protections against continuously emerged SARS-CoV-2 mutant strains.
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http://dx.doi.org/10.1016/j.gendis.2021.05.006DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8240504PMC
June 2021

[Screening and quantitative analysis of nine illicit antiallergics in emulsion cosmetics by ultra-high performance liquid chromatography-quadrupole-time-of-flight high-resolution mass spectrometry].

Se Pu 2020 Dec;38(12):1423-1430

Wuhan Institute for Food and Cosmetic Control, Wuhan 430014, China.

A rapid and accurate analysis method based on ultra-high performance liquid chromatography coupled with quadrupole-time-of-flight high-resolution mass spectrometry (UPLC-Q-TOF-HRMS) was developed to screen and determine nine antiallergy drugs in emulsion cosmetics. First, a standard library of the target compounds was established. The library contained the TOF-MS information and secondary MS information such as retention time, ion addition mode, mass error, isotope distribution, mass-to-charge ratio of the parent ion, and fragment ion distribution. According to the European Union regulation (SANTE/11945/2015), the standard for the qualitative determination by HRMS was determined; that is, each compound was confirmed by two ions with a mass error below 5%, and the abundance ratio of the two ions was less than 30%. Second, the instrument conditions and sample pretreatment conditions for the determination of different compounds were optimized, and the influence of different levels of quantitative ions on the matrix effect was compared. The following observations were made:(1) the addition of 0.1% formic acid to the water phase improved the response of the chromatographic peaks; (2) among the various solvent amounts tested (4, 5, 6, 8 mL acetonitrile and 4, 5, 6, 8 mL methanol), 4 mL acetonitrile showed the best extraction efficiency; (3) PRiME HLB had a better purification effect than the other two purification columns (C18 and HLB solid-phase extraction cartridges), thus reducing the interference of impurities and ensuring good recovery of the target compounds; (4) the use of two pairs of secondary product ion quantification could significantly reduce the matrix effect of anti-allergic compounds and improve the quantification accuracy. Finally, based on the above findings, the experimental procedure was established. The lotion samples were first ultrasonically extracted with acetonitrile and purified on the PRiME HLB column. Chromatographic separation was performed on a Waters XBridge C18 column with gradient elution using 0.1% (v/v) formic acid in water and acetonitrile. Finally, the sequential window acquisition of all theoretical mass spectra (SWATH), which shows obvious advantages in continuous and high-throughput acquisition, was selected for MS data acquisition. The retention time, mass accuracy, isotope distribution, and fragment ion matching ratio were used for fast qualitative screening, while the peak areas of characteristic product ions were used for precise quantification. All the calibration curves showed good linearity (>0.99) within the tested ranges (5-100 μg/L) under the optimum conditions. The limits of quantification (LOQs) were in the range of 0.05-0.10 mg/kg. The recoveries were in the range of 65.3%-107% at three spiked levels (0.10, 0.20, and 0.60 mg/kg), with relative standard deviations (RSDs, =6) below 20%. Compared with the existing ion exchange column methods, the proposed "one-step" purification method based on PRiME HLB is simpler and more rapid, where the extraction solution is filtered directly after allowing it to pass through the column, without any subsequent washing and elution procedures. In addition, the LOQs of this method are lower than those of other LC-MS/MS methods, indicating that the proposed method has higher sensitivity. The application of SWATH data acquisition makes it possible to achieve quantification with two pairs of product ions, thus reducing the matrix effect and ensuring accuracy of the quantitative results. Therefore, the proposed method is less time-consuming and operationally convenient, and it can be used for the rapid screening and accurate quantification of antiallergics in lotion samples.
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http://dx.doi.org/10.3724/SP.J.1123.2020.06017DOI Listing
December 2020

Improvement of Metabolic Syndrome in High-Fat Diet-Induced Mice by Yeast β-Glucan Is Linked to Inhibited Proliferation of and in Gut Microbiota.

J Agric Food Chem 2021 Jul 1;69(27):7581-7592. Epub 2021 Jul 1.

Institute of Marine Drugs, Guangxi University of Chinese Medicine, Nanning 530200, Guangxi, China.

There is growing evidence that prevention of metabolic syndrome (MS) by dietary fibers is intricately linked to gut microbiota. In the present work, the mice were fed a high-fat diet (HFD) and orally treated with yeast β-glucan to further examine the effects of β-glucan on MS and gut microbiota and the potential relationship between gut microbiota and its activity. After intervention for 10 weeks, it was found that the treatment of yeast β-glucan could significantly improve the HFD-induced MS. Furthermore, pro-inflammatory cytokines in plasma including IL-6 and IL-1β were decreased. Yeast β-glucan could regulate the diversity and composition of HFD-induced gut microbiota. Moreover, the relative abundances of and , having significant positive correlation with metabolic changes, were decreased by β-glucan, which might play a critical role in attenuation of MS. Our findings suggest that yeast β-glucan shows promising application as a prebiotic for preventing MS and regulating gut microbiota.
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http://dx.doi.org/10.1021/acs.jafc.1c00866DOI Listing
July 2021

Application of Vinamidinium Salt Chemistry for a Palladium Free Synthesis of Anti-Malarial MMV048: A "Bottom-Up" Approach.

Org Lett 2021 Jul 29;23(14):5400-5404. Epub 2021 Jun 29.

Medicines for All Institute, 737 North Fifth Street, Box 980100, Richmond, Virginia 23298, United States.

MMV390048 () is a clinical compound under investigation for antimalarial activity. A new synthetic route was developed which couples two aromatic fragments while forming the central pyridine ring over two steps. This sequence takes advantage of raw materials used in the existing etoricoxib supply chain and eliminates the need for palladium catalysts, which were projected to be major cost-drivers.
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http://dx.doi.org/10.1021/acs.orglett.1c01725DOI Listing
July 2021

Effect of using Celosia argentea grown from seeds treated with a magnetic field to conduct Cd phytoremediation in drought stress conditions.

Chemosphere 2021 Oct 3;280:130724. Epub 2021 May 3.

College of Resources and Environment, Yangtze University, Wuhan, China. Electronic address:

The mechanisms of the stimulatory effect of external magnetic fields on plant growth have been revealed; however, the role of magnetic fields in the efficiency of phytoremediation with Celosia argentea grown under drought stress which results in detrimental influences on food security has not been reported. Therefore, this study evaluated the physiological responses of C. argentea to the interactions between exposure to a magnetic field and drought stress. Compared with a control, a drought treatment negatively affected the dry weight, transpiration rate, and Cd extraction efficiency of the species and caused oxidative damage in plant cells, as manifested by the increase in malondialdehyde levels and antioxidant enzyme activities. The biomass production, pigment levels, Cd content, and phytoremediation efficiency of the plant were positively affected by all magnetic field treatments compared to the control. All magnetic treatments, except those at 30 mT, alleviated the detrimental effects induced by a 10-day irrigation regime by enhancing the dry weight, chlorophyll content, and activities of antioxidant enzymes in the leaves of the plant. In terms of the interaction between pre-sowing magnetic field seed treatment and drought stress, a 100 mT treatment increased most of the measured parameters, particularly under a 3-day irrigation regime; this corresponded to the optimal phytoremediation efficiency. The results suggest that magnetic field treatment is a novel, economical, and practicable strategy by which to increase the efficiency of phytoremediation using C. argentea under drought stress.
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http://dx.doi.org/10.1016/j.chemosphere.2021.130724DOI Listing
October 2021

Safety and efficacy of S1 monotherapy or combined with nab-paclitaxel in advanced elderly pancreatic cancer patients: A meta-analysis.

Medicine (Baltimore) 2021 Jun;100(25):e26342

Department of Hepatobiliary Surgery, the First Affiliated Hospital of Dalian Medical University.

Objective: To evaluate the therapeutic efficacy and safety of S1 monotherapy or combination with nab-paclitaxel for the treatment of elderly patients with metastatic or locally advanced pancreatic adenocarcinoma.

Method: PubMed, Embase, Cochrane Central Library, China Biology Medicine, and China National Knowledge Infrastructure databases were searched without time limits according to the inclusion criteria. RevMan (Version 5.3) software was used for data extraction and meta-analysis. Objective response rate (ORR) and disease control rate (DCR) were used to evaluate therapeutic effects while side effects including leukopenia, thrombocytopenia, neurotoxicity, vomit, and alopecia were extracted for evaluation. There was no need for ethical review in this study because no ethical experiments were conducted and all data used were public data. All relevant data are within the paper and its Supporting Information files.

Results: Four retrospective studies comprising 308 elderly patients with metastatic or locally advanced pancreatic adenocarcinoma were included in the analysis. One hundred fifty-one patients underwent S1 monotherapy and 157 received S1 combined nab-paclitaxel. Meta-analysis indicated that compared with S1 monotherapy, S1 combined with nab-paclitaxel had higher ORR (OR 2.25, 95% CI: 1.42-3.55; P = .0005) and DCR (OR 2.94, 95% CI: 1.55-5.58; P = .0009). The adverse reaction of leukopenia was higher in the combined therapy group (OR 1.85, 95% CI: 1.09-3.13, P = .02), but no significant difference was found in thrombocytopenia, neurotoxicity, vomiting, and alopecia between the 2 groups (P > .05).

Conclusion: Nab-paclitaxel plus S1 was more efficient in terms of ORR and DCR than S1 monotherapy in elderly pancreatic ductal adenocarcinoma patients while the side effect was controllable with a higher probability of leukopenia. Thus, combined nab-paclitaxel and S1 could be safely used in elderly patients.
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http://dx.doi.org/10.1097/MD.0000000000026342DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8238291PMC
June 2021

Engineering circular RNA regulators to specifically promote circular RNA production.

Theranostics 2021 24;11(15):7322-7336. Epub 2021 May 24.

Second Affiliated Hospital, Institute of Cancer Stem Cell, Dalian Medical University, Dalian, China 116044.

A large number of circular RNAs (circRNAs) have been discovered in the mammalian transcriptome with high abundance, which play vital roles in gene regulation, thereby participating in the development of multiple diseases. However, the biogenesis, regulation, and especially manipulation of circRNAs still remain largely unknown. Engineering circRNA regulators (ECRRs) were developed to promote circRNA biogenesis. Multiple circRNA mini-gene reporters were generated to evaluate the regulatory role of ECRRs. RT-PCR, qRT-PCR, northern blot, western blot, and flow cytometry assays were applied to assess the efficiency of artificial circRNA regulators on circRNA production in the presence or absence of RNase R treatment. We engineered circRNA regulators by combining sequence-specific RNA binding motifs of human Pumilio 1 with functional domains that could form dimerization. We applied these engineered regulators to promote the circRNA production of the exogenous circRNA minigene reporter circGFP, thereby stimulating the functional GFP protein generation. Crucially, such regulation is in time-course dependent and dose-dependent manners with designed specificity. Moreover, the application of ECRRs could also stimulate circRNA biogenesis of another minigene reporter circScreen, suggesting that ECRRs can be commonly used to promote circRNA generation of exogenous reporters. Most importantly, ECRRs could be utilized to specifically promote the production of the endogenous circRNAs circ10720 and circBIRC6 as well. Our approach allows the creation of engineered regulators to target virtually any pre-mRNA , offering a novel avenue to investigate circRNA biogenesis and manipulate disease-related circRNA production.
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http://dx.doi.org/10.7150/thno.56990DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8210604PMC
July 2021

[Effect of hypoxia inducible factor 1α overexpression on differentiation of stem cells derived from human exfoliated deciduous teeth into vascular endothelial cells].

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2021 Jun;35(6):761-768

Department of Stomatology, Qingyang District Hospital of Traditional Chinese Medicine, Chengdu Sichuan, 610036, P.R.China.

Objective: To investigate the effects of hypoxia inducible factor 1α (HIF-1α) overexpression on the differentiation of stem cells derived from human exfoliated deciduous teeth (SHED) into vascular endothelial cells.

Methods: SHED was isolated from the retained primary teeth donated by healthy children by using collagenase digestion method. The third generation cells were identified by flow cytometry and alizarin red and alkaline phosphatase (ALP) staining after osteogenic differentiation culture. The SHED were divided into blank control group (SHED without any treatment), empty group (SHED infected with empty lentivirus), HIF-1α overexpression group (SHED infected with HIF-1α overexpression lentivirus), Wnt inhibitor group (SHED interfered by IWR-1), and combination group (HIF-1α overexpressed SHED interfered by IWR-1). Real-time fluorescence quantitative PCR (qRT-PCR) and Western blot were used to analyze the expressions of HIF-1α mRNA and protein in the SHED of blank control group, empty group, and HIF-1α overexpression group. Then the SHED in 5 groups were induced differentiation into vascular endothelial cells for 14 days. The expressions of cell surface marker molecule [von Willebrand factor (vWF) and CD31] were detected by flow cytometry. The mRNA expressions of vascular cell adhesion protein 1 (VCAM-1), KDR (Kinase-inserted domain containing receptor), and VE-cadherin (VE) were analyzed by qRT-PCR. The protein expressions of phosphate-glycogen synthasc kinase 3β (p-GSK3β) and β-catenin were analyzed by Western blot. The tube forming ability of induced cells was detected by Matrigel tube forming experiment. The ability of endothelial cells to phagocytic lipid after differentiation was detected by DiI-labeled acetylated low density lipoprotein (DiI-Ac-LDL) phagocytosis.

Results: After identification, the cells were SHED. After lentivirus transfection, compared with the blank control group and the empty group, the expressions of HIF-1α mRNA and protein in the HIF-1α overexpression group increased significantly ( <0.05). Compared with the blank control group and the empty group, the expressions of VCAM-1, KDR, and VE mRNA, the percentages of vWF positive cells and CD31 positive cells, and the relative expression of β-catenin protein were significantly higher ( <0.05), the relative expression of p-GSK3β protein was significantly lower ( <0.05), the number of tubules formed and the ability to phagocytic lipids significantly increased ( <0.05) in the HIF-1α overexpression group; while the indicators in the Wnt inhibitor group were opposite to those in the HIF-1α overexpression group ( <0.05). Compared with the HIF-1α overexpression group, the expressions of VCAM-1, KDR, and VE mRNA, the percentages of vWF positive cells and CD31 positive cells, and the relative expression of β-catenin protein were significantly lower ( <0.05), the relative expression of p-GSK3β protein was significantly higher, and the number of tubules formed and the ability of phagocytosis of lipids significantly reduced, showing significant differences between groups ( <0.05).

Conclusion: Overexpression of HIF-1α can promote SHED to differentiate into vascular endothelial cells by activating Wnt/β-catenin signaling pathway.
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http://dx.doi.org/10.7507/1002-1892.202012024DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8218175PMC
June 2021

EGCG synergizes the therapeutic effect of irinotecan through enhanced DNA damage in human colorectal cancer cells.

J Cell Mol Med 2021 Jun 16. Epub 2021 Jun 16.

Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu, China.

Irinotecan is a kind of alkaloid with antitumour activity, but its low solubility and high toxicity limit its application. Epigallocatechin-3-gallate (EGCG) is one of the main bioactive components in tea. The epidemiological investigation and animal and cell experiments show that EGCG has a preventive and therapeutic effect on many kinds of tumours. Here, colorectal cancer cells RKO and HCT116 were employed, and the CCK8 proliferation test was used to screen the appropriate concentration of EGCG and irinotecan, and the effects of single and/or combined drugs on migration, invasion, DNA damage, cell cycle and autophagy of tumour cells were investigated. The results showed that EGCG combined with irinotecan (0.5 μmol L ) not only had a stronger inhibitory effect on tumour cells than EGCG or irinotecan alone but also prevented tumour cell migration and invasion. EGCG alone did not cause DNA damage in colorectal cancer cells, but its combination with irinotecan could induce S or G2 phase arrest by inhibiting topoisomerase I to cause more extensive DNA damage. EGCG also induced apoptosis by promoting autophagy with irinotecan synergistically. These results indicated that EGCG in combination with irinotecan could be a promising strategy for colorectal cancer.
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http://dx.doi.org/10.1111/jcmm.16718DOI Listing
June 2021

Ultrasonic Features of Uncommon Congenital Heterotopic Colon and Pancreas in the Neck: An Extremely Rare Case Report.

Front Pediatr 2021 28;9:655142. Epub 2021 May 28.

Department of Ultrasound, Guangdong Women and Children Hospital, Guangzhou, China.

Congenital heterotopic colon and pancreas localized to the neck has not been reported. Herein, we describe an extremely uncommon case of congenital heterotopic colon and pancreas aberrantly presented within a cyst on the neck, and the thickened wall of the cyst on ultrasound may represent an important ultrasonic feature.
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http://dx.doi.org/10.3389/fped.2021.655142DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8192969PMC
May 2021

The relation between aortic arch branching types and the location of large vessel occlusion in cardioembolic stroke.

J Clin Neurosci 2021 Jul 12;89:171-176. Epub 2021 May 12.

Chongqing Key Laboratory of Cerebrovascular Disease Research, No. 439, Xuanhua Road, Yongchuan, Chongqing 402160, People's Republic of China; Department of Neurology, Yongchuan Hospital of Chongqing Medical University, No. 439, Xuanhua Road, Yongchuan, Chongqing 402160, People's Republic of China; Department of Internal Medicine, Changdu People's Hospital, Changdu 854000, People's Republic of China. Electronic address:

Cardiac embolism is the leading etiology of ischemic strokes. There are arguments about the left-right propensity of cardioembolic strokes.This study aimed to reveal the relationship between the different aortic arch types and the location of large vessel occlusion (LVO) in cardioembolic stroke.We retrospectively identified all patients with acute ischemic stroke admitted to our comprehensive stroke center who had medium- to high-risk cardioembolicsources according to the TOAST classification.Only those with LVO and available images of the aortic arch were included. Patients were classified into 3 groups according to the aortic arch types: Type I (n = 44), Type II (n = 105), Type III (n = 36).The thrombus was divided into large thrombus or small thrombus based on the location of LVO.Overall, left-sided strokes (50.8%) were almost equal to right-sided (49.2%). There was a growing tendency for the percentage of left-sided infarcts with advancement of the aortic arch types either in the total cases or in the atrial fibrillation cases, with no statistical difference between the 3 aortic arch types.In type III aortic arch, left-sided strokes (69.0%) were twice than right-sided (31%) in large thrombus (P < 0.05), while right-sided strokes (85.7%) were more common than left-sided (14.3%) in small thrombus (P < 0.05).Conversely, in type Ⅰ and II aortic arches, left-sided strokes were more common than right-sided in small thrombus, while right-sided strokes were more common than left-sided in large thrombus (P < 0.05). The left-right propensity of cardioembolic stroke is related to the proximity of clot lodging in different aortic arch types.
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http://dx.doi.org/10.1016/j.jocn.2021.05.013DOI Listing
July 2021

Children ASD Evaluation Through Joint Analysis of EEG and Eye-Tracking Recordings With Graph Convolution Network.

Front Hum Neurosci 2021 25;15:651349. Epub 2021 May 25.

School of Computer Science, Wuhan University, Wuhan, China.

Recent advances in neuroscience indicate that analysis of bio-signals such as rest state electroencephalogram (EEG) and eye-tracking data can provide more reliable evaluation of children autism spectrum disorder (ASD) than traditional methods of behavior measurement relying on scales do. However, the effectiveness of the new approaches still lags behind the increasing requirement in clinical or educational practices as the "bio-marker" information carried by the bio-signal of a single-modality is likely insufficient or distorted. This study proposes an approach to joint analysis of EEG and eye-tracking for children ASD evaluation. The approach focuses on deep fusion of the features in two modalities as no explicit correlations between the original bio-signals are available, which also limits the performance of existing methods along this direction. First, the synchronization measures, information entropy, and time-frequency features of the multi-channel EEG are derived. Then a random forest applies to the eye-tracking recordings of the same subjects to single out the most significant features. A graph convolutional network (GCN) model then naturally fuses the two group of features to differentiate the children with ASD from the typically developed (TD) subjects. Experiments have been carried out on the two types of the bio-signals collected from 42 children (21 ASD and 21 TD subjects, 3-6 years old). The results indicate that (1) the proposed approach can achieve an accuracy of 95% in ASD detection, and (2) strong correlations exist between the two bio-signals collected even asynchronously, in particular the EEG synchronization against the face related/joint attentions in terms of covariance.
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http://dx.doi.org/10.3389/fnhum.2021.651349DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8185139PMC
May 2021
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