Publications by authors named "Dan Cai"

58 Publications

Rice Potassium Transporter OsHAK8 Mediates K Uptake and Translocation in Response to Low K Stress.

Front Plant Sci 2021 3;12:730002. Epub 2021 Aug 3.

Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA, United States.

Potassium (K) levels in the soil often limit plant growth and development. As a result, crop production largely relies on the heavy use of chemical fertilizers, presenting a challenging problem in sustainable agriculture. To breed crops with higher K-use efficiency (KUE), we must learn how K is acquired from the soil by the root system and transported to the rest of the plant through K transporters. In this study, we identified the function of the rice K transporter , whose expression level is downregulated in response to low-K stress. When was disrupted by CRISPR/Cas9-mediated mutagenesis, mutant plants showed stunted growth, especially under low-K conditions. Ion content analyses indicated that K uptake and root-to-shoot K transport were significantly impaired in mutants under low-K conditions. As the gene was broadly expressed in different cell types in the roots and its protein was targeted to the plasma membrane, we propose that OsHAK8 serves as a major transporter for both uptake and root-to-shoot translocation in rice plants.
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http://dx.doi.org/10.3389/fpls.2021.730002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8369890PMC
August 2021

Relationship between self-efficacy, social rhythm, and mental health among college students: a 3-year longitudinal study.

Curr Psychol 2021 Aug 14:1-10. Epub 2021 Aug 14.

Education College, Shanghai Normal University, No. 100, Guilin Road, Shanghai, 200234 China.

Although many empirical studies have aimed to find variances in positive mental health among different groups and the relationship between mental health and other variables, few studies examined the developmental trend of mental health levels and the cross-lagged relationship between self-efficacy, social rhythm, and mental health. Furthermore, few of them explored the interrelationship between self-efficacy and social rhythm among college students over time. This study thus aimed to clarify the longitudinal cross-lagged relationship between self-efficacy, social rhythm, and positive mental health among college students. A total of 764 students (627 females, mean age 21.03 ± 0.84 years at T1) participated in a 3-year-long study (T1, T2, T3) and were asked to complete the General Self-Efficacy, the Brief Social Rhythm, and the Positive Mental Health Scales. The results indicate that the development of positive mental health among college students showed an upward trend during those 3 years. There was a significant relationship between positive mental health, social rhythm, and self-efficacy. Positive mental health and social rhythm significantly predicted self-efficacy in the following year. Positive mental health in T2 could predict both T3 social rhythm marginally and T3 self-efficacy significantly. However, T1 social rhythm was not indirectly associated with T3 mental health via T2 self-efficacy, and T1 self-efficacy was not indirectly associated with T3 mental health via T2 social rhythm. This finding clarified the relationship between positive mental health, self-efficacy, and social rhythm, and provided evidence that positive mental health is the basis for self-efficacy and social rhythm among young adults. Therefore, school psychologists in universities should pay close attention to the positive mental health of young adults to form high levels of self-efficacy and social rhythm.
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http://dx.doi.org/10.1007/s12144-021-02160-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8364412PMC
August 2021

The effects of working memory training in children revealed by behavioral responses and ERP.

Brain Behav 2021 08 1;11(8):e2310. Epub 2021 Aug 1.

Department of Psychology, Shanghai Normal University, Shanghai, China.

Background: Recent studies have examined the effect of computerized cognitive training on working memory (WM), but the behavioral and neural effects were uncertain. Also, few studies have explored WM training effects on children using event-related potentials. The purpose of our study was to investigate the effects of WM training in children, including the effects on behavioral performance and neurophysiological outcomes.

Methods: Forty-four healthy children (mean age = 7.76 years, SD = 0.57 years, 18 females) were assigned to the training and control groups. Over 20 training sessions, the training group participated in the computation-span and spatial N-back tasks, whereas the control group joined in normal class activities. They all completed the pre- and post-test evaluation of WM tasks (digit span backwards task and N-back task).

Results: The results showed that WM training led to improved performance in the digit span backwards task and 2-back task of post-test evaluation, shortened P3a and P3b latencies in nontarget trials during the spatial 1-back task, shortened P3a latency in target and nontarget trials, as well as increased P3b amplitude and shortened P3b latency in target trials during the spatial 2-back task.

Conclusions: These results suggested that WM training might enhance children's behavioral performance on WM tasks and brought about neurophysiological changes. This study gives insights into the potential of WM training effects on children's behavioral performance and neurophysiological outcomes.
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http://dx.doi.org/10.1002/brb3.2310DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8413785PMC
August 2021

The value of circulating fibrinogen-to-pre-albumin ratio in predicting survival and benefit from chemotherapy in colorectal cancer.

Ther Adv Med Oncol 2021 28;13:17588359211022886. Epub 2021 Jun 28.

Biological Resource Center, The Second Affiliated Hospital of Nanchang University, No.1 of Minde Road, Nanchang, 330006, China.

Background: To evaluate the prognostic role of circulating fibrinogen-to-pre-albumin (FPR) in colorectal cancer (CRC) with different tumor locations, and its involvement in chemosensitivity and chemoresistance.

Patients And Methods: A total of 2917 eligible CRC patients from multiple centers were enrolled in this prospective study, and 3 years follow-up was carried out to obtain the outcome of these patients. Circulating fibrinogen (Fib), pre-albumin (pAlb), carcinoembryonic antigen (CEA), and carbohydrate antigen 19-9 (CA19-9) were detected, and we calculated FPR according to the detected results. Kaplan-Meier curves, Cox proportional regression, time-dependent receiver operating characteristic curves, Harrell's concordance index, calibration, and decision curves were used to investigate the role of FPR in predicting chemotherapy efficacy and prognosis of CRC patients.

Results: Our results showed that cancer bulk, its infiltrating depth, and the distal metastasis status of CRC determined circulating FPR levels. A high FPR was associated with a significantly inferior prognosis, while the outcomes of right-sided patients with stage III and IV CRC were worse than left-sided cases. Only FPR was found to be a reliable and independent prognostic factor for each stage of CRC. In addition, the prognostic FPR-contained nomograms were superior to the non-FPR nomograms and FPR in predicting the outcomes in both localized and metastatic CRC patients. The circulating FPR was significantly associated with chemotherapeutic efficacy in stage III and IV CRC patients. In particular, low-grade (FPR < 15) and medium-grade (15 ⩽ FPR < 20) FPR patients exhibited a complete response to chemotherapy and attenuated chemosensitivity, respectively; in contrast, high-grade inflammation (FPR ⩾ 20) conferred resistance to the treatment.

Conclusion: Circulating FPR is a robust and independent prognostic factor, a simple and economically-friendly predictor of chemotherapy efficacy within cases of localized and metastatic CRC. FPR-contained nomograms are more effective in predicting the prognosis of these patients. FPR and the nomogram can be recommended for the evaluation of chemotherapy efficacy and to aid decision-making associated with the management of these patients.
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http://dx.doi.org/10.1177/17588359211022886DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8243139PMC
June 2021

Characterization of the distribution, source, and potential ecological risk of perfluorinated alkyl substances (PFASs) in the inland river basin of Longgang District, South China.

Environ Pollut 2021 Oct 23;287:117642. Epub 2021 Jun 23.

GMU-GIBH Joint School of Life Sciences, Guangzhou Medical University, Guangzhou, 511436, PR China.

Previous studies of perfluorinated alkyl substances (PFASs) in receiving water bodies of typical industrial parks under the low-carbon development mode are scarce. In the present study, 18 PFASs were analyzed in surface water and sediment samples of the inland river basin in Longgang District in 2017. The ΣPFAS concentrations in surface water (drought and rainy periods) and sediment ranged from 15.17 to 948.50 ng/L, 11.56-561.14 ng/L, and 1.07-28.94 ng/g dw, respectively. Perfluorooctane sulfonate (PFOS), perfluorooctanoic acid (PFOA) and perfluorobutane sulfonate (PFBS) were the dominant pollutants in surface water, with maximum concentrations of 867.68 ng/L, 288.28 ng/L, and 245.09 ng/L, respectively. Meanwhile, PFOS, perfluoroundecanoic acid (PFUdA), PFBS, and perfluorodecanoic acid (PFDA) were the major PFASs in the sediment samples, with maximum concentrations of 9.83 ng/g dw, 11.86 ng/g dw, 5.30 ng/g dw, and 5.23 ng/g dw, respectively. In addition, PFOA and PFOS resulted from similar sources in sediment and surface water samples (P < 0.05). The risk quotient value (RQ) results showed that the control of PFOS in the treatment of pollutants in the inland river basin of Longgang District deserves more attention.
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http://dx.doi.org/10.1016/j.envpol.2021.117642DOI Listing
October 2021

Economic evaluation of remdesivir for the treatment of severe COVID-19 patients in China under different scenarios.

Br J Clin Pharmacol 2021 Apr 14. Epub 2021 Apr 14.

School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, China.

Aims: The present study aimed to evaluate the cost-effectiveness of the 5-day remdesivir regimen compared with standard of care among severe COVID-19 patients in China, the evidence on which is essential to inform the necessity of securing access to remdesivir.

Methods: A dynamic transmission model that extended the susceptible-exposed-infected-recovered framework by incorporating asymptomatic, presymptomatic and waiting-to-be-diagnosed patients was constructed to conduct the cost-effectiveness analysis from the healthcare system perspective. To estimate epidemic parameters, the model was first calibrated to the observed epidemic curve in Wuhan from 23 January to 19 March 2020. Following the calibration, the infected compartment was replaced by 3 severity-defined health states to reflect differential costs and quality of life associated with disease gravity. Costs and quality-adjusted life year (QALY) outcomes of 9 million simulated people were accrued across time to evaluate the incremental cost-effectiveness ratio of remdesivir. As robustness checks, an alternative modelling technique using decision tree, additional epidemic scenarios representing different epidemic intensities, and 1-way parameter variations were also analysed.

Results: Remdesivir treatment cost CN¥97.93 million more than standard of care. Also, the net QALY gain from 5-day remdesivir treatment was 6947 QALYs. As such, the incremental cost-effectiveness ratio was CN¥14 098/QALY, substantially lower than the gross domestic product per capita threshold. The peak daily number of severe cases was 19% lower in the remdesivir treatment strategy. Overall, results were robust in alternative scenarios and sensitivity analyses.

Conclusion: Given the cost-effectiveness profile, access to remdesivir for severe COVID-19 patients in China should be considered.
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http://dx.doi.org/10.1111/bcp.14860DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8251382PMC
April 2021

A towering genome: Experimentally validated adaptations to high blood pressure and extreme stature in the giraffe.

Sci Adv 2021 Mar 17;7(12). Epub 2021 Mar 17.

School of Ecology and Environment, Northwestern Polytechnical University, Xi'an 710072, China.

The suite of adaptations associated with the extreme stature of the giraffe has long interested biologists and physiologists. By generating a high-quality chromosome-level giraffe genome and a comprehensive comparison with other ruminant genomes, we identified a robust catalog of giraffe-specific mutations. These are primarily related to cardiovascular, bone growth, vision, hearing, and circadian functions. Among them, the giraffe gene is an outlier with seven unique amino acid substitutions not found in any other ruminant. Gene-edited mice with the giraffe-type show exceptional hypertension resistance and higher bone mineral density, both of which are tightly connected with giraffe adaptations to high stature. Our results facilitate a deeper understanding of the molecular mechanism underpinning distinct giraffe traits, and may provide insights into the study of hypertension in humans.
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http://dx.doi.org/10.1126/sciadv.abe9459DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7968835PMC
March 2021

Lucidumones B-H, racemic meroterpenoids that inhibit tumor cell migration from Ganoderma lucidum.

Bioorg Chem 2021 05 24;110:104774. Epub 2021 Feb 24.

School of Pharmaceutical Sciences, Shenzhen University Health Science Center, Shenzhen 518060, People's Republic of China. Electronic address:

Seven new meroterpenoids, lucidumones B-H (1 and 3-8), along with one known meroterpenoid (2), were isolated from the fruiting bodies of Ganoderma lucidum. The structures of the new compounds were assigned by spectroscopic and computational methods. All the isolated compounds were tested for their inhibition on human cancer cell migration. It was found that compounds (-)-1, (+)-2, (-)-4, (+)-6, and (+)-8 could significantly inhibit cell migration in KYSE30 cell line. Further examination disclosed that cell migration inhibition of (+)-6 and (+)-8 might be related with downregulation of N-cadherin.
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http://dx.doi.org/10.1016/j.bioorg.2021.104774DOI Listing
May 2021

The relationship of resting-state EEG oscillations to executive functions in middle childhood.

Int J Psychophysiol 2021 06 26;164:64-70. Epub 2021 Feb 26.

Department of Occupational Therapy, University of Alberta, Edmonton, Canada; Neuroscience and Mental Health Institute, University of Alberta, Edmonton, Canada. Electronic address:

Executive functions (EFs) play important roles in children's development, but their neural mechanisms are rarely investigated, especially for the different components of EFs in middle childhood. Therefore, this study aimed to explore the links between resting-state EEG in the frontal scalp region and EFs in children aged 7-9 years. Fifty-nine typically developing children from the second and third grades performed two core EF tasks, i.e., inhibition and working memory, and a high-level EF task, i.e., planning, followed by the recording of EEG signals during eyes-open and eyes-closed resting states. The results showed that distinct EEG activities in the frontal scalp region predicted different EF components. More specifically, after controlling for age and verbal ability, alpha to theta power ratio (ATR) and beta to theta power ratio (BTR) during the eyes-open resting state positively predicted inhibition, and beta to theta power ratio (BTR) during the eyes-open resting state positively predicted planning. However, we did not find any EEG features related to working memory. Our results contributed to the understanding of inter-individual differences in EFs and provided insights into the regulation of corresponding EEG activities through EEG neurofeedback for enhancing children's EFs.
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http://dx.doi.org/10.1016/j.ijpsycho.2021.02.021DOI Listing
June 2021

Translation and Validation of Fear of Pain-9 Items into Simplified Chinese Version for Mainland China.

J Pain Res 2021 12;14:35-40. Epub 2021 Jan 12.

Nursing Department, Zhongnan Hospital of Wuhan University, Wuhan, People's Republic of China.

Purpose: This study aimed to obtain a translation and validation of the Fear of Pain Questionnaire 9 Items (FOP-9) into simplified Chinese.

Methods: The questionnaire was translated following the forward-backward method. The final version was filled out by (n = 300) patients. Cronbach's coefficient was calculated to test the internal consistency of simplified Chinese version of FOP-9 (sc-FOP-9), and 50 painless patients completed the sc-FOP-9 questionnaire within a 2-weeks interval to evaluate test-retest reliability. To verify the construct validity, exploratory factor analysis was used to explore the factor structure, and confirmatory factor analysis was conducted to evaluate the goodness fit of models.

Results: Satisfactory psychometric qualities were obtained (Cronbach's α of the total score was 0.873 and intraclass correlation coefficient was 0.975). Three first-order models were tested and all show a good model fit and the 3-factor structure may be better due to its higher factor loading.

Conclusion: The sc-FOP-9 is a reliable and valid instrument to evaluate the fear of pain among Chinese patients with or without pain. Fear of pain may have an important effect on perioperative pain and chronic pain, and this tool is a good complement to the measurement in mainland China.
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http://dx.doi.org/10.2147/JPR.S275227DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7811576PMC
January 2021

Extracellular matrix scaffold crosslinked with vancomycin for multifunctional antibacterial bone infection therapy.

Biomaterials 2021 01 17;268:120603. Epub 2020 Dec 17.

Department of Orthopaedic Surgery, Sir Run Run Shaw Hospital, Medical College of Zhejiang University, Hangzhou, China; Key Laboratory of Musculoskeletal System Degeneration and Regeneration Translational Research of Zhejiang Province, China. Electronic address:

The treatment of acute and chronic bone infections remains a major clinical challenge. The various factors released by the bacteria, acidic environment, and bacterial colonies in the bone grooves and implanted synthetic materials collectively promote the formation of biofilms. Dormant bacteria and biofilms cause infections that are difficult to cure and that can develop chronically. Therefore, a new antibacterial material was synthesized in the present study for multifunctional bone infection therapy and consists of specific demineralized extracellular cancellous bone (SDECM) crosslinked with vancomycin (Van) by means of electrostatic interactions and chemical bonds. It was verified in vitro that the new material (Van-SDECM) not only has pH-sensitive release and biofilm inhibition properties, but also maintains sustained bactericidal ability accompanied by the degradation of the scaffold, which does not affect its favorable osteogenic performance. The infectious bone defect in vivo model further confirms the comprehensive anti-infective and osteogenic ability of the Van-SDECM. Further, these favorable properties are due to the pH-sensitive sustained release sterilization and scaffold contact antibacterial properties, accompanied by osteoclast activity inhibition, osteogenesis promotion and immunoregulation effects. This study provides a new drug-scaffold composite preparation method based on a native-derived extracellular matrix scaffold.
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http://dx.doi.org/10.1016/j.biomaterials.2020.120603DOI Listing
January 2021

[A case of glycogen storage disease type Ⅰa with gout as the main clinical manifestation].

Zhonghua Yi Xue Yi Chuan Xue Za Zhi 2020 Oct;37(10):1162-1166

Department of Endocrinology and Metabolism, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, China.

Objective: To explore the genetic etiology of a patient with glycogen accumulation type Ⅰa with gout as the main clinical feature.

Methods: Clinical data of the patient was collected. The patient and her parents were subjected to next generation sequencing (NGS). Suspected pathogenic variation was verified by Sanger sequencing.

Results: The patient, a 30-year-old women, mainly manifested hyperuricemia, chronic gouty arthritis, fasting hypoglycemia, hypertriglyceridemia, hyperlactatemia, hepatomegaly, urolithiasis, and gradually developed liver nodules and renal dysfunction. NGS revealed that she has carried c.648G>T (exon 5) and c.260delG (exon 2) compound heterozygous variants of the G6PC gene, which were respectively inherited from her father (phenotypically normal) and mother (with hyperuricemia). The c.260delG variant was unreported previously. Bioinformatic analysis indicated that both variants are pathogenic.

Conclusion: The compound heterozygous variants of the G6PC gene probably underlay the glycogen storage disease Ⅰa in this patient. G6PC gene mutations should be excluded in young women with hyperuricemia and /or gout.
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http://dx.doi.org/10.3760/cma.j.cn511374-20190518-00249DOI Listing
October 2020

Effectiveness of remdesivir for the treatment of hospitalized COVID-19 persons: A network meta-analysis.

J Med Virol 2021 02 30;93(2):1171-1174. Epub 2020 Sep 30.

School of Population and Public Health, University of British Columbia, Vancouver, British Columbia, Canada.

Several randomized clinical trials (RCTs) that investigated the effectiveness of remdesivir for the treatment of coronavirus disease-2019 (COVID-19) have generated inconsistent evidence. The present study aimed to synthesize available RCT evidence using network meta-analyses (NMAs). Both blinded and open-label RCTs in PubMed database from inception to 7 June 2020 that contained "remdesivir", "Covid-19", and "trial" in the abstracts conducted on hospitalized COVID-19 persons were identified and screened. The studies must have at least one remdesivir arm and evaluated one of the pre-specified outcomes. The outcomes were clinical improvement between days 10 to 15 after randomization and clinical recovery during the follow-up period. The identified literature was supplemented with relatively recent studies that were known to the researchers if not already included. Frequentist NMAs with random effects were conducted. Both 10-day and 5-day remdesivir regimens were associated with higher odds of clinical improvement (odds ratio [OR] of 10-day regimen: 1.35, 95% confidence interval [CI], 1.09-1.67); OR of 5-day regimen: 1.81, 95% CI, 1.32-2.45, and higher probabilities of clinical recovery (relative risk [RR] of 10-day regimen: 1.24, 95% CI, 1.07-1.43; RR of 5-day regimen: 1.47, 95% CI, 1.16-1.87 compared with placebo. Remdesivir may have clinical benefits among hospitalized COVID-19 persons.
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http://dx.doi.org/10.1002/jmv.26443DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7461548PMC
February 2021

Effect of pullulan concentration and pH on the interactions between whey protein concentrate and pullulan during gelation.

J Sci Food Agric 2021 Jan 26;101(2):659-665. Epub 2020 Aug 26.

College of Food Science and Engineering, Jilin Agricultural University, Changchun, China.

Background: Whey protein concentrate (WPC)/pullulan (PUL) hydrogel is applied as a microencapsulation wall material to protect probiotics. However, the interactions between WPC and PUL during gelation have not been clarified. In the present study, the effects of PUL concentration and pH on the interactions between WPC and PUL during gelation were evaluated with respect to appearance, zeta-potential, sulfhydryl group amount, surface hydrophobicity and infrared spectroscopy measurements. The rheological properties of WPC/PUL gels were also determined.

Results: The results obtained showed that a proper concentration (0.40 g mL ) of PUL could improve the gel by enhancing the strength of hydrogen bonding, electrostatic interactions and exposure of hydrophobic groups, whereas too much PUL inhibited the formation of disulfide bonds. Furthermore, hydrophobic interactions, disulfide bonds and hydrogen bonds were destroyed in varying degrees under an alkaline environment. The rheological results also demonstrated a similar effect of PUL concentration and pH on the storage modulus (G') of WPC/PUL gels.

Conclusion: When the WPC/PUL gel was formed at PUL concentration of 0.40 g mL and pH 7.0, the interaction between WPC and PUL could be enhanced, which is beneficial for the future application of WPC/PUL gels in the food industry. © 2020 Society of Chemical Industry.
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http://dx.doi.org/10.1002/jsfa.10678DOI Listing
January 2021

The cost-effectiveness of conducting three versus two reverse transcription-polymerase chain reaction tests for diagnosing and discharging people with COVID-19: evidence from the epidemic in Wuhan, China.

BMJ Glob Health 2020 07;5(7)

School of Population and Public Health, The University of British Columbia, Vancouver, British Columbia, Canada.

Objectives: The objectives were to evaluate the effectiveness of conducting three versus two reverse transcription-PCR (RT-PCR) tests for diagnosing and discharging people with COVID-19 with regard to public health and clinical impacts by incorporating asymptomatic and presymptomatic infection and to compare the medical costs associated with the two strategies.

Methods: A model that consisted of six compartments was built. The compartments were the susceptible (S), the asymptomatic infective (A), the presymptomatic infective (L), the symptomatic infective (I), the recovered (R), and the deceased (D). The A, L and I classes were infective states. To construct the model, several parameters were set as fixed using existing evidence and the rest of the parameters were estimated by fitting the model to a smoothed curve of the cumulative confirmed cases in Wuhan from 24 January 2020 to 6 March 2020. Input data about the cost-effectiveness analysis were retrieved from the literature.

Results: Conducting RT-PCR tests three times for diagnosing and discharging people with COVID-19 reduced the estimated total number of symptomatic cases to 45‌ 013 from 51 144 in the two-test strategy over 43 days. The former strategy also led to 850.1 quality-adjusted life years (QALYs) of health gain and a net healthcare expenditure saving of CN¥49.1 million. About 100.7 QALYs of the health gain were attributable to quality-adjusted life day difference between the strategies during the analytic period and 749.4 QALYs were attributable to years of life saved.

Conclusions: More accurate strategies and methods of testing for the control of COVID-19 may reduce both the number of infections and the total medical costs. Increasing the number of tests should be considered in regions with relatively severe epidemics when existing tests have moderate sensitivity.
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http://dx.doi.org/10.1136/bmjgh-2020-002690DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7385750PMC
July 2020

Endoscopic Management of Ganglioneuroma in the Stomach.

ACG Case Rep J 2020 May 6;7(5):e00382. Epub 2020 May 6.

Division of Gastroenterology and Hepatology, Case Western Reserve University at MetroHealth Medical Center, Cleveland, OH.

Ganglioneuromas are rare benign tumors of neuroblastic origin that can occur anywhere along the sympathetic chain. They are rarely found in the gastrointestinal tract and can appear as solitary polypoid lesions or diffuse as part of a familial syndrome. They are often asymptomatic but may present with nonspecific symptoms depending on their anatomic location. They have no special endoscopic appearance and are diagnosed by histology and immunohistochemistry staining. We present a case of solitary gastric ganglioneuroma treated successfully with endoscopic resection.
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http://dx.doi.org/10.14309/crj.0000000000000382DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7289278PMC
May 2020

Preparation and characterization of a novel porous whey protein concentrate/pullulan gel induced by heating for Cu absorption.

Food Chem 2020 Aug 7;322:126772. Epub 2020 Apr 7.

College of Resources and Environment, Jilin Agriculture University, Changchun, China. Electronic address:

Whey protein concentrate (WPC) and pullulan (PUL) were used to prepare a hydrogel (WPC/PUL) as a novel Cu adsorbent. The scanning electron microscopy, rheology, zeta-potential, sulfhydryl groups, surface hydrophobicity, infrared spectroscopy and thermal gravimetric analysis measurements were used to characterize WPC/PUL. The results showed the mechanism of gel formation may depend on the combination of electrostatic interaction, disulfide bond, hydrophobic interaction and hydrogen bond. The drying kinetic test was taken, and the drying process of WPC/PUL followed Page Model and the activation energy was 56.73 kJ/mol. Furthermore, WPC/PUL was used as a Cu adsorbent and the absorption can reach more than 98% of the equilibrium adsorption (Q) after shaking in Cu solution for 10 min and Q based on the pseudo-second-order kinetic model was 81.66 mg/g. Because the materials were safe and non-toxic, WPC/PUL could be used as a Cu adsorbent during the food industry production process in the future.
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http://dx.doi.org/10.1016/j.foodchem.2020.126772DOI Listing
August 2020

Transplacental Transfer of Per- and Polyfluoroalkyl Substances (PFASs): Differences between Preterm and Full-Term Deliveries and Associations with Placental Transporter mRNA Expression.

Environ Sci Technol 2020 04 1;54(8):5062-5070. Epub 2020 Apr 1.

School of Environment and Guangdong Key Laboratory of Environmental Pollution and Health, Jinan University, Guangzhou 510632, China.

Previous studies demonstrated that per- and polyfluoroalkyl substances (PFASs) can cross the human placental barrier. However, their transplacental transfer efficiencies (TTEs) have not been investigated in preterm delivery, and the role of placental transport proteins has rarely been explored. Our study hypothesized that the TTEs of PFASs could differ between preterm and full-term deliveries, and some placental transporters could be involved in active maternofetal PFAS transfer. In the present study, the median TTEs of 16 individual PFAS chemicals or isomers were determined to be 0.23 to 1.72 in matched maternal-cord serum pairs with preterm delivery ( = 86), which were significantly lower than those (0.35 to 2.26) determined in full-term delivery ( = 187). Significant associations were determined between the TTEs of several PFASs and the mRNA expression levels of selected transporters located on the brush border membrane. The association patterns also significantly differed between preterm and full-term deliveries and exhibited a chemical-specific manner. For example, the expression of exhibited significantly positive associations with the TTEs of linear and branched perfluorooctanesulfonic acid (PFOS) isomers in full-term delivery, but negative, nonsignificant associations were observed in preterm delivery. This is the first study to compare the transplacental transfer of PFASs between preterm and full-term deliveries and indicate that some placental transport proteins could be involved in active transmission. The mechanisms underlying the cross-placental transfer of PFASs require further investigations to better elucidate their risks to fetal health and birth outcomes.
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http://dx.doi.org/10.1021/acs.est.0c00829DOI Listing
April 2020

Meroterpenoids from the Fungus and First Absolute Configuration Clarification of Zizhine H.

Molecules 2019 Dec 31;25(1). Epub 2019 Dec 31.

School of Pharmaceutical Sciences, Shenzhen University Health Science Center, Shenzhen 518060, China.

Five new meroterpenoids, zizhines P-S and U (-,), together with two known meroterpenoids ( and ) were isolated from . Their structures including absolute configurations were assigned by using spectroscopic, computational, and chemical methods. Racemics zizhines P and Q were purified by HPLC on chiral phase. Biological evaluation found that , and are cytotoxic toward human cancer cells (A549, BGC-823, Kyse30) with IC values in the range of 63.43-80.83 μM towards A549, 59.2 ± 2.73 μM and 64.25 ± 0.37 μM towards BGC-823, 76.28 ± 1.93 μM and 85.42 ± 2.82 μM towards Kyse30.
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http://dx.doi.org/10.3390/molecules25010158DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6983046PMC
December 2019

High trans-placental transfer of perfluoroalkyl substances alternatives in the matched maternal-cord blood serum: Evidence from a birth cohort study.

Sci Total Environ 2020 Feb 2;705:135885. Epub 2019 Dec 2.

Guangdong Provincial Engineering Technology Research Center of Environmental Pollution and Health Risk Assessment, Department of Occupational and Environmental Health, School of Public Health, Sun Yat-sen University, Guangzhou 510080, China. Electronic address:

Background: Recent studies suggest that perfluoroalkyl substances (PFAS) and PFAS alternatives can cross the placental barrier. However, little is known on the differential patterns of trans-placental transfer (TPT) among conventional PFAS and PFAS alternatives in epidemiological study.

Objectives: We aimed to characterize comprehensive TPT patterns in conventional PFAS and PFAS alternatives using matched maternal-cord blood serum from a birth cohort.

Methods: A total of 424 mother-fetus pairs were recruited from the Maoming Birth Cohort during 2015-2018. We detected 20 PFAS in cord and maternal serum using an ultraperformance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). TPT of PFAS was calculated via cord to maternal serum concentration ratios.

Results: Both of PFOS alternatives (chlorinated polyfluorinated ether sulfonates, Cl-PFESAs) and PFOA short-chain alternative (perfluorobutanoic acid, PFBA) were widely detected in the cord and maternal serum. In cord serum, the predominant PFAS was PFOS (1.93 ng/mL), followed by PFBA (1.45 ng/mL), PFOA (0.75 ng/mL) and 6:2 Cl-PFESA (0.32 ng/mL). We found that the PFAS alternatives had higher TPT than PFOS and PFOA, such as PFBA vs. PFOA (median: 1.41 vs. 0.73, P < 0.001) and 8:2 Cl-PFESA vs. PFOS (median: 0.98 vs. 0.42, P < 0.001). Moreover, the TPT of 8:2 Cl-PFESA was higher than the precursor, linear and isomeric PFOS, respectively (P < 0.01). Furthermore, we found a U-shaped pattern for TPT in perfluorocarboxylic acid compounds (PFCAs) across different length of carbon chain.

Conclusion: Our findings suggest that PFAS alternatives may be more easily across the placenta than conventional PFAS. Given the widespread usage of PFAS alternatives, our results indicate that more research is needed to assess the potential health risks of prenatal exposure to PFAS alternatives in children.
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http://dx.doi.org/10.1016/j.scitotenv.2019.135885DOI Listing
February 2020

Influence of multiple freezing/thawing cycles on a structural, rheological, and textural profile of fermented and unfermented corn dough.

Food Sci Nutr 2019 Nov 13;7(11):3471-3479. Epub 2019 Oct 13.

College of Food Science and Engineering Jilin Agricultural University Changchun China.

In the current study, the impact of fermentation and freezing/thawing treatment on corn flour was studied. Fermentation revealed an increase (12%) in amylose content, while freezing reflected a loss of amylose. The results of scanning electron microscope (SEM) revealed more grooves, indentations, and the irregular shape of particles. Rapid Visco Analyzer (RVA) exhibited different pasting behavior on the dough. The molecular structure had similar profiles but showed several discernible absorbance at the different wavelengths. Differential scanning calorimetry (DSC) showed an increase in melting temperature range due to fermentation and freezing/thawing treatment attributed to more heterogeneous morphology. Overall, the results of this research showed the insight alterations that induce the changes in corn flour leading to improvement in some properties and it may enhance the acquaintance about the upright revolution in the profile of corn dough and its potential usage in industry and homes.
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http://dx.doi.org/10.1002/fsn3.1193DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6848845PMC
November 2019

Effect on Viability of Microencapsulated with the Whey Protein-pullulan Gels in Simulated Gastrointestinal Conditions and Properties of Gels.

Food Sci Anim Resour 2019 Jun 30;39(3):459-473. Epub 2019 Jun 30.

College of Food Science and Engineering, Jilin Agricultural University, Changchun, China.

GG (LGG) has low resistance to low pH and bile salt in the gastrointestinal juice. In this study, the gel made from whey protein concentrate (WPC) and pullulan (PUL) was used as the wall material to prepare the microencapsulation for LGG protection. The gelation process was optimized and the properties of gel were also determined. The results showed the optimal gel was made from 10% WPC and 8.0% PUL at pH 7.5, which could get the best protective effect; the viable counts of LGG were 6.61 Log CFU/g after exposure to simulated gastric juice (SGJ) and 9.40 Log CFU/g to simulated intestinal juice (SIJ) for 4 h. Sodium dodecyl sulphite polyacrylamide gel electrophoresis (SDS-PAGE) confirmed that the WPC-PUL gel had low solubility in SGJ, but dissolved well in SIJ, which suggested that the gel can protect LGG under SGJ condition and release probiotics in the SIJ. Moreover, when the gel has highest hardness and water-holding capacity, the viable counts of LGG were not the best, suggesting the relationship between the protection and the properties of the gel was non-linear.
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http://dx.doi.org/10.5851/kosfa.2019.e42DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6612782PMC
June 2019

Ginsenoside Rg2 inhibits adipogenesis in 3T3-L1 preadipocytes and suppresses obesity in high-fat-diet-induced obese mice through the AMPK pathway.

Food Funct 2019 Jun;10(6):3603-3614

College of Life Science, Jilin Agricultural University, Changchun, Jilin 130118, China.

Ginsenoside Rg2 is one of the specific ginsenosides in red ginseng, and has been reported to exhibit protective effects against neurotoxicity and memory impairment, and also inhibition of hepatic glucose production. However, the effect of Rg2 on the prevention of obesity has not been investigated. In this study, we evaluated the anti-obesity and anti-adipogenic effects of Rg2 in high-fat diet-induced obese mice (HFD mice) and 3T3-L1 preadipocytes. Oral administration of Rg2 (10 mg kg-1) to HFD mice significantly decreased body weight gain, total triglycerides, and free fatty acid levels. In 3T3-L1 preadipocytes, Rg2 (80 μM) inhibited adipocyte differentiation and reduced the accumulation of intracellular lipids. Quantitative PCR and western blot analysis revealed that Rg2 decreased the expression levels of adipogenic transcription factors (PPARγ, C/EBPα, and SREBP1-c), and then regulated target genes such as acetyl-CoA carboxylase (ACC) and fatty acid synthase (FAS). Rg2 significantly promoted AMP-activated protein kinase (AMPK) both in vivo and in vitro, which is known to suppress adipogenesis. It was also found that pretreating with compound C, a typical inhibitor of AMPK, attenuated the inhibitory effect of Rg2 on AMPK phosphorylation. These findings suggested that Rg2-induced activation of AMPK leads to a decrease in the expression of adipogenic transcription factors, and suppression of adipogenesis in vivo and in vitro. Hence, Rg2 has the potential for the development of healthy foods and the prevention of obesity.
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http://dx.doi.org/10.1039/c9fo00027eDOI Listing
June 2019

Adrenal cavernous hemangioma: A rare tumor that mimics adrenal cortical carcinoma.

Surg Open Sci 2019 Jul 27;1(1):7-13. Epub 2019 Apr 27.

Department of Surgery, MetroHealth Medical Center, Case Western Reserve University School of Medicine, Cleveland, OH.

Background: Adrenal cavernous hemangioma is a rare tumor with only 60 cases previously reported. The aim of this study was to determine the frequency and clinical significance of adrenal cavernous hemangioma at our institution.

Methods: A retrospective review of consecutive patients undergoing adrenalectomy from 1994 to 2018 was completed to determine the frequency of cavernous hemangioma, characterize the clinical presentation, imaging and pathologic features and review the operative management and outcome.

Results: Of 144 consecutive patients who underwent adrenalectomy by a single surgeon, 5 (3.5%) had an adrenal cavernous hemangioma. All were incidentally discovered, nonfunctional adrenal masses varying in size from 7 to 12 cm with imaging features that were indeterminate in differentiating a benign adenoma from an adrenal cortical carcinoma. Attenuation values for the adrenal masses on noncontrast computed tomography varied from 28 to 34 Hounsfield units. All adrenal cavernous hemangiomas were large, heterogeneous, complex masses with a variable presence of calcification, hemorrhage, and necrosis. These features, along with tumor enlargement were concerning for adrenal cortical carcinoma. During the operation, there was no local invasion and all adrenal tumors were successfully removed laparoscopically without tumor rupture or bleeding. All patients had an uneventful postoperative course without complications.

Conclusion: Adrenal cavernous hemangioma is a rare tumor that can grow to a very large size without causing symptoms, making it difficult to differentiate from adrenal cortical carcinoma clinically or radiographically. Despite its large size, adrenal cavernous hemangioma can be safely resected laparoscopically.
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http://dx.doi.org/10.1016/j.sopen.2019.04.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7391906PMC
July 2019

Zeaxanthin promotes mitochondrial biogenesis and adipocyte browning via AMPKα1 activation.

Food Funct 2019 Apr;10(4):2221-2233

College of Food Science and Engineering, Jilin Agricultural University, Changchun, Jilin 130118, China.

Zeaxanthin (ZEA), a type of oxygenated carotenoid with strong antioxidant activity, has previously been found to exhibit an anti-lipogenesis effect. In the present study, we investigated the effect of ZEA on brown-like adipocyte formation and mitochondrial biogenesis in 3T3-L1 adipocytes. Brown adipocyte-specific markers, mitochondrial biogenesis and oxidative stress, and the involvement of AMP-activated protein kinase (AMPK) α1 were assessed. ZEA treated adipocytes demonstrated a brown-like pattern, with upregulated expression of uncoupling protein 1 (UCP1) and other brown adipocyte markers. In addition, ZEA intervention induced a dramatic increase in mitochondrial DNA (mtDNA) content and in the mRNA levels of genes associated with mitochondrial biogenesis. Furthermore, ZEA attenuated mitochondrial oxidative damage caused by lipid peroxidation in adipocytes, significantly improved the mitochondrial membrane potential (MMP), and scavenged intracellular reactive oxygen species (ROS) and mitochondrial superoxide. Finally, we concluded that AMPKα1 mediated the ZEA-caused inhibition of lipid accumulation and promotion of brown and beige adipocyte-biomarker expression, as the positive effects of ZEA were diminished by Prkaa1 (AMPKα1) knockdown. These findings demonstrated that ZEA promoted the expression of brown and beige adipogenesis markers and mitochondrial biogenesis, which involved AMPKα1 activation, thus contributing to the anti-obesity effects of ZEA.
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http://dx.doi.org/10.1039/c8fo02527dDOI Listing
April 2019

Measurement Invariance of the Depression Anxiety Stress Scales-21 Across Gender in a Sample of Chinese University Students.

Front Psychol 2018 31;9:2064. Epub 2018 Oct 31.

Department of Clinical Psychology and Psychotherapy, Mental Health Research and Treatment Center, Ruhr-Universität Bochum, Bochum, Germany.

The Depression Anxiety Stress Scales-21 (DASS-21) has three 7-item subscales (depression, anxiety, and stress). The current study aims assess the gender-based measurement invariance of the DASS-21 questionnaire in a Chinese university student sample from five different cities. The sample was composed of 13208 participants (62.3% female, mean age of 19.7 years, and age = 1.8). Multi-group confirmatory factor analysis supported full measurement invariance for the three subscales. The findings support the measurement invariance of DASS-21 scores across gender. Future research on the DASS should include additional validation across ethnicities and testing of all versions of the DASS.
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http://dx.doi.org/10.3389/fpsyg.2018.02064DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6220040PMC
October 2018

The Role of Approximate Number System in Different Mathematics Skills Across Grades.

Front Psychol 2018 18;9:1733. Epub 2018 Sep 18.

Department of Educational Psychology, University of Alberta, Edmonton, AB, Canada.

Although approximate number system (ANS) has been found to predict mathematics ability, it remains unclear if both aspects of ANS (symbolic and non-symbolic estimation) contribute equally well to mathematics performance and if their contribution varies as a function of the mathematics outcome and grade level. Thus, in this study, we examined the effects of both aspects of ANS on different mathematics skills across three grade levels. Three hundred eleven children (100 children from kindergarten, 107 children from Grade 2, and 104 children from Grade 4) from two kindergartens and three elementary schools in Shanghai, China, were assessed on measures of ANS (dot estimation and number line estimation), general cognitive ability (nonverbal intelligence, inhibition, and working memory), and mathematics abilities (numerical operations and mathematical problem solving in all grades, early mathematical skills in kindergarten, and calculation fluency in Grades 2 and 4). Results of hierarchical regression analyses showed that, in kindergarten, non-symbolic estimation predicted all mathematics skills even after controlling for age, gender, and general cognitive ability. In Grades 2 and 4, symbolic estimation accounted for unique variance in mathematical problem solving, but not in calculation fluency. Symbolic estimation also predicted numerical operations in Grade 4. Taken together, these findings suggest that in the early phases of mathematics development different aspects of ANS contribute to different mathematics skills.
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http://dx.doi.org/10.3389/fpsyg.2018.01733DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6153330PMC
September 2018

Antiobesity Effects of Ginsenoside Rg1 on 3T3-L1 Preadipocytes and High Fat Diet-Induced Obese Mice Mediated by AMPK.

Nutrients 2018 Jun 27;10(7). Epub 2018 Jun 27.

National Engineering Laboratory for Wheat and Corn Deep Processing, Changchun, Jilin 130118, China.

Ginsenosides Rg1 is one of the major pharmacologically active saponins in ginseng, which as an antioxidant reduces oxidative damage in the liver and can also be used to prevent cardiovascular diseases and diabetes. However, there is no research targeting the effect of lipid metabolism in high-fat diet (HFD)-induced mice. In this study, we evaluated the anti-obesity effects of Rg1 in 3T3-L1 adipocyte cells and HFD-induced obese C57BL/6J mice. Administration of Rg1 to HFD-induced obese mice significantly decreased body weight, total cholesterol, and total triglyceride levels. In addition to effects in 3T3-L1 cells, Rg1 reduced the accumulation of lipid droplets in a dose-dependent manner. Furthermore, Rg1 exhibits an anti-adipogenic effect via regulation of the expression of the transcriptional factors and lipid metabolism-related genes in vivo and in vitro. We observed that Rg1 administration significantly increased the phosphorylation level of AMP-activated protein kinase (AMPK) in both epididymal white adipose tissue and 3T3-L1 cells. These results indicated that Rg1 works both in an anti-adipogenic and anti-obesity manner through inducing AMPK activation, inhibiting lipogenesis, and decreasing intracellular lipid content, adipocyte size, and adipose weight.
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http://dx.doi.org/10.3390/nu10070830DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6073290PMC
June 2018

A novel uromodulin mutation in autosomal dominant tubulointerstitial kidney disease: a pedigree-based study and literature review.

Ren Fail 2018 Nov;40(1):146-151

a Department of Endocrinology and Metabolism , West China Hospital of Sichuan University , Chengdu , P. R. China.

Autosomal dominant tubulointerstitial kidney disease caused by mutations in uromodulin gene (ADTKD-UMOD) is a spectrum of hereditary renal disorders, characterized by early-onset hyperuricemia, gout and progressive nephropathy. This study presented a novel UMOD mutation in an ADTKD pedigree and reviewed studies in Chinese population. The index patient is a 16-year-old girl with hypertension, hyperuricemia and normal serum creatinine level. Four affected and six unaffected members were available for genetic screen. The mutation analysis was performed by next-generation sequencing and direct sequencing. A literature research was conducted to review Chinese ADTKD-UMOD cases. MEDLINE and Chinese Biomedicine Databases were searched with 'uromodulin', 'juvenile gout' and their related terms. Genetic sequencing revealed a de novo mutation within exon 3 (Cys223Gly), which was co-segregating with phenotype in this pedigree. In the review, four studies and our study involving a total of 67 ADTKD patients from 11 families were identified. Of these patients, 27 were confirmed to carry UMOD mutations. Mutations occurred in exon 3 were commonly observed, while mutations within exon 4, 5 and 9 occurred less frequently in Chinese ADTKD-UMOD cases. Among these cases, median age of symptom onset was 26.5 years, median age of end-stage renal diseases (ESRD) or death by ESRD was 41.9 years without renal replacement treatment. Phenotype caused by mutations in D8C domain seemed to be severe than those in GPI domain. Compared with patients of other race, Chinese ADTKD-UMOD patients advanced more aggressively to ESRD.
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http://dx.doi.org/10.1080/0886022X.2018.1450757DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6014484PMC
November 2018

Number Line Estimation Predicts Mathematical Skills: Difference in Grades 2 and 4.

Front Psychol 2017 12;8:1576. Epub 2017 Sep 12.

Department of Occupational Therapy, Neuroscience and Mental Health Institute, University of Alberta, EdmontonAB, Canada.

Studies have shown that number line estimation is important for learning. However, it is yet unclear if number line estimation predicts different mathematical skills in different grades after controlling for age, non-verbal cognitive ability, attention, and working memory. The purpose of this study was to examine the role of number line estimation on two mathematical skills (calculation fluency and math problem-solving) in grade 2 and grade 4. One hundred and forty-eight children from Shanghai, China were assessed on measures of number line estimation, non-verbal cognitive ability (non-verbal matrices), working memory (N-back), attention (expressive attention), and mathematical skills (calculation fluency and math problem-solving). The results showed that in grade 2, number line estimation correlated significantly with calculation fluency ( = -0.27, < 0.05) and math problem-solving ( = -0.52, < 0.01). In grade 4, number line estimation correlated significantly with math problem-solving ( = -0.38, < 0.01), but not with calculation fluency. Regression analyses indicated that in grade 2, number line estimation accounted for unique variance in math problem-solving (12.0%) and calculation fluency (4.0%) after controlling for the effects of age, non-verbal cognitive ability, attention, and working memory. In grade 4, number line estimation accounted for unique variance in math problem-solving (9.0%) but not in calculation fluency. These findings suggested that number line estimation had an important role in math problem-solving for both grades 2 and 4 children and in calculation fluency for grade 2 children. We concluded that number line estimation could be a useful indicator for teachers to identify and improve children's mathematical skills.
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http://dx.doi.org/10.3389/fpsyg.2017.01576DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5600960PMC
September 2017
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