Damien Marsic, PhD - Medimmune - Scientist II

Damien Marsic

PhD

Medimmune

Scientist II

Gaithersburg, MD | United States

Main Specialties: Biotechnology

Additional Specialties: Directed evolution, Biotechnology, Molecular Biology, Gene therapy, AAV

Damien Marsic, PhD - Medimmune - Scientist II

Damien Marsic

PhD

Introduction

Primary Affiliation: Medimmune - Gaithersburg, MD , United States

Specialties:

Additional Specialties:

Research Interests:

Publications

17Publications

367Reads

3Profile Views

91PubMed Central Citations

Direct Head-to-Head Evaluation of Recombinant Adeno-associated Viral Vectors Manufactured in Human versus Insect Cells.

Mol Ther 2017 Dec 10;25(12):2661-2675. Epub 2017 Aug 10.

Department of Pediatrics, University of Florida College of Medicine, Gainesville, FL 32610, USA. Electronic address:

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http://dx.doi.org/10.1016/j.ymthe.2017.08.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5768557PMC
December 2017
39 Reads
6.230 Impact Factor

An Immune-Competent Murine Model to Study Elimination of AAV-Transduced Hepatocytes by Capsid-Specific CD8 T Cells.

Mol Ther Methods Clin Dev 2017 Jun 19;5:142-152. Epub 2017 Apr 19.

Department of Pediatrics, University of Florida, Gainesville, FL 32610, USA.

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http://dx.doi.org/10.1016/j.omtm.2017.04.004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5415329PMC
June 2017
33 Reads

Evaluation of engineered AAV capsids for hepatic factor IX gene transfer in murine and canine models.

J Transl Med 2017 05 1;15(1):94. Epub 2017 May 1.

Department of Pediatrics, University of Florida, Gainesville, FL, 32610, USA.

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http://dx.doi.org/10.1186/s12967-017-1200-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5412045PMC
May 2017
30 Reads
3 Citations
3.930 Impact Factor

Altering Tropism of rAAV by Directed Evolution.

Methods Mol Biol 2016 ;1382:151-73

Department of Pediatrics, College of Medicine, University of Florida, 2033 Mowry Road, CGRC 235, Gainesville, FL, 32610, USA.

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http://dx.doi.org/10.1007/978-1-4939-3271-9_11DOI Listing
October 2016
20 Reads
1 Citation

High-accuracy biodistribution analysis of adeno-associated virus variants by double barcode sequencing.

Mol Ther Methods Clin Dev 2015 28;2:15041. Epub 2015 Oct 28.

Department of Pediatrics, Division of Cell & Molecular Therapy, University of Florida , Gainesville, Florida, USA.

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http://dx.doi.org/10.1038/mtm.2015.41DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4701405PMC
January 2016
12 Reads
2 Citations

High-accuracy biodistribution analysis of adeno-associated virus variants by double barcode sequencing

Molecular Therapy — Methods & Clinical Development 2, Article number: 15041 (2015) ​doi:10.1038/

Molecular Therapy — Methods & Clinical Development

Biodistribution analysis is a key step in the evaluation of adeno-associated virus (AAV) capsid variants, whether natural isolates or produced by rational design or directed evolution. Indeed, when screening candidate vectors, accurate knowledge about which tissues are infected and how efficiently is essential. We describe the design, validation, and application of a new vector, pTR-UF50-BC, encoding a bioluminescent protein, a fluorescent protein and a DNA barcode, which can be used to visualize localization of transduction at the organism, organ, tissue, or cellular levels. In addition, by linking capsid variants to different barcoded versions of the vector and amplifying the barcode region from various tissue samples using barcoded primers, biodistribution of viral genomes can be analyzed with high accuracy and efficiency.

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October 2015
38 Reads

Vector design Tour de Force: integrating combinatorial and rational approaches to derive novel adeno-associated virus variants.

Mol Ther 2014 Nov 22;22(11):1900-9. Epub 2014 Jul 22.

Department of Pediatrics, Division of Cell & Molecular Therapy, University of Florida, Gainesville, Florida, USA.

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http://dx.doi.org/10.1038/mt.2014.139DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4429732PMC
November 2014
22 Reads
13 Citations
6.230 Impact Factor

Proteocatella sphenisci gen. nov., sp. nov., a psychrotolerant, spore-forming anaerobe isolated from penguin guano.

Int J Syst Evol Microbiol 2009 Sep 20;59(Pt 9):2302-7. Epub 2009 Jul 20.

NASA/NSSTC, Astrobiology Laboratory, 320 Sparkman Drive, Room 4247, Huntsville, AL 35805, USA.

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http://dx.doi.org/10.1099/ijs.0.002816-0DOI Listing
September 2009
73 Reads
6 Citations
2.511 Impact Factor

Spirochaeta dissipatitropha sp. nov., an alkaliphilic, obligately anaerobic bacterium, and emended description of the genus Spirochaeta Ehrenberg 1835.

Int J Syst Evol Microbiol 2009 Jul;59(Pt 7):1798-804

NASA/NSSTC, Astrobiology Laboratory, 320 Sparkman Drive, Huntsville, AL 35805, USA.

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http://dx.doi.org/10.1099/ijs.0.65862-0DOI Listing
July 2009
11 Reads
8 Citations
2.511 Impact Factor

New DNA polymerase from the hyperthermophilic marine archaeon Thermococcus thioreducens.

Extremophiles 2008 Nov 1;12(6):775-88. Epub 2008 Aug 1.

HudsonAlpha Institute for Biotechnology, 601 Genome Way, Huntsville, AL 35806, USA.

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http://dx.doi.org/10.1007/s00792-008-0181-7DOI Listing
November 2008
7 Reads
1 Citation
2.310 Impact Factor

PCR-based gene synthesis to produce recombinant proteins for crystallization.

BMC Biotechnol 2008 Apr 29;8:44. Epub 2008 Apr 29.

ExtremoZyme Inc, HudsonAlpha Institute for Biotechnology, 601 Genome Way, Huntsville, AL 35806, USA.

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http://dx.doi.org/10.1186/1472-6750-8-44DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2408586PMC
April 2008
33 Reads
5 Citations
2.034 Impact Factor

Thermococcus thioreducens sp. nov., a novel hyperthermophilic, obligately sulfur-reducing archaeon from a deep-sea hydrothermal vent.

Int J Syst Evol Microbiol 2007 Jul;57(Pt 7):1612-8

Astrobiology Laboratory, NASA/NSSTC, VP62, 320 Sparkman Dr., Huntsville, AL 35805, USA.

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http://dx.doi.org/10.1099/ijs.0.65057-0DOI Listing
July 2007
41 Reads
13 Citations
2.511 Impact Factor

Trichococcus patagoniensis sp. nov., a facultative anaerobe that grows at -5 degrees C, isolated from penguin guano in Chilean Patagonia.

Int J Syst Evol Microbiol 2006 Sep;56(Pt 9):2055-62

NASA/NSSTC, VP-62, Astrobiology Laboratory, 320 Sparkman Dr., Huntsville, AL 35805, USA.

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http://dx.doi.org/10.1099/ijs.0.64225-0DOI Listing
September 2006
7 Reads
3 Citations
2.511 Impact Factor

Carnobacterium pleistocenium sp. nov., a novel psychrotolerant, facultative anaerobe isolated from permafrost of the Fox Tunnel in Alaska.

Int J Syst Evol Microbiol 2005 Jan;55(Pt 1):473-8

NASA/NSSTC, XD12, 320 Sparkman Drive, Room 4247, Huntsville, AL 35805, USA.

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http://dx.doi.org/10.1099/ijs.0.63384-0DOI Listing
January 2005
12 Reads
7 Citations
2.511 Impact Factor

Desulfonatronum thiodismutans sp. nov., a novel alkaliphilic, sulfate-reducing bacterium capable of lithoautotrophic growth.

Int J Syst Evol Microbiol 2003 Sep;53(Pt 5):1327-32

Astrobiology Laboratory, NASA/NSSTC, 320 Sparkman Drive, Huntsville, AL 35805, USA.

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http://dx.doi.org/10.1099/ijs.0.02598-0DOI Listing
September 2003
12 Reads
16 Citations
2.511 Impact Factor

Tindallia californiensis sp. nov., a new anaerobic, haloalkaliphilic, spore-forming acetogen isolated from Mono Lake in California.

Extremophiles 2003 Aug 1;7(4):327-34. Epub 2003 May 1.

SD-50, NASA/National Space Science and Technology Center, 320 Sparkman Drive, Huntsville AL 35805, USA.

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http://dx.doi.org/10.1007/s00792-003-0326-7DOI Listing
August 2003
7 Reads
6 Citations
2.310 Impact Factor

Spirochaeta americana sp. nov., a new haloalkaliphilic, obligately anaerobic spirochaete isolated from soda Mono Lake in California.

Int J Syst Evol Microbiol 2003 May;53(Pt 3):815-21

NASA/NSSTC, SD-50, 320 Sparkman Dr., Huntsville, AL 35805, USA.

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http://dx.doi.org/10.1099/ijs.0.02535-0DOI Listing
May 2003
8 Reads
7 Citations
2.511 Impact Factor