Publications by authors named "Daichi Takagi"

18 Publications

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Ruptured type A aortic dissection with saddle pulmonary embolism-like appearance on computed tomography angiography.

J Card Surg 2021 May 30. Epub 2021 May 30.

Department of Cardiovascular surgery, Akita University Graduate School of Medicine, Akita, Japan.

We present a case of ruptured type A acute aortic dissection with pulmonary trunk compression by mediastinal hematoma. This condition mimicked a pulmonary embolism on the early arterial phase of computed tomography angiography, whereas the correct diagnosis was determined from the late arterial phase and unenhanced computed tomography. We highlight the importance of carefully interpreting triphasic computed tomography angiography to assess the status of aortic dissection.
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http://dx.doi.org/10.1111/jocs.15691DOI Listing
May 2021

Avoiding anticoagulation drugs for postoperative atrial fibrillation enabled successful conservative treatment of left atrial dissection: a case report.

Surg Case Rep 2021 May 12;7(1):120. Epub 2021 May 12.

Department of Cardiovascular Surgery, Akita University Graduate School of Medicine, Hondo 1-1-1, Akita, 010-8543, Japan.

Background: Left atrial dissection is a rare complication of cardiac surgery, most commonly associated with mitral valve surgery. Herein, we report on the successful conservative treatment of left atrial dissection while avoiding anticoagulation therapy.

Case Presentation: A 64-year-old man developed left atrial dissection during operation for acute type A aortic dissection, most likely due to retrograde cardioplegia cannulation. As there was no connection between the left atrial dissection cavity and the left atrium on enhanced computed tomography, we did not administer anticoagulants to prevent expansion of the left atrial dissection cavity. However, the patient developed atrial fibrillation, which was successfully managed by beta-blocker and amiodarone administration. Follow-up imaging showed gradual left atrial dissection reduction, and the patient was started on anticoagulation therapy.

Conclusion: We were able to resolve left atrial dissection by preventing the use of anticoagulation therapy in the acute stage by managing the atrial fibrillation with antiarrhythmic drugs.
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http://dx.doi.org/10.1186/s40792-021-01194-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8116416PMC
May 2021

[Traction-assisted Negative Pressure Wound Therapy for Pediatric Poststernotomy Mediastinitis:Report of Two Cases].

Kyobu Geka 2021 Feb;74(2):121-124

Department of Cardiovascular Surgery, Akita University, Akita, Japan.

We herein report two cases of pediatric poststernotomy mediastinitis treated by traction-assisted negative pressure wound therapy (NPWT) with Zip Surgical Skin Closure (Zip), which is a non-invasive skin closure device. We used this device with NPWT in cases of pediatric poststernotomy mediastinitis to stabilize the sternum and reduce the natural retractive forces of the skin. The patients were two boys (two and three months old), with an onset of infection at 13 and eight postoperative days, respectively. The culture examination detected methicillin-susceptible Staphylococcus aureus in both cases. Traction-assisted NPWT with Zip was performed at-75 mmHg for 16 and 33 days, and the wounds healed completely. In conclusion, this modification was successfully applied to treat pediatric poststernotomy mediastinitis and may help reduce the duration of treatment.
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February 2021

Secretory production of a camelid single-domain antibody (VHH, nanobody) by the Serratia marcescens Lip system in Escherichia coli.

Biochem Biophys Res Commun 2021 Apr 2;549:105-112. Epub 2021 Mar 2.

Department of Biological Science and Technology, Tokushima University Graduate School, Minamijosanjima-cho, Tokushima, 770-8506, Japan. Electronic address:

Escherichia coli is one of the most popularly used hosts to produce recombinant proteins. Most recombinant proteins are produced in the cytoplasm and periplasm, requiring multiple steps to extract and purify recombinant proteins. The Serratia marcescens Lip system (LipB-LipC-LipD) is a type 1 secretion system that selectively secretes LipA from the intracellular to extracellular space in a single step. This study aimed to establish a secretory production system for nanobodies, camelid-derived small molecule antibody fragments, using the S. marcescens Lip system. Surprisingly, E. coli harboring only LipC, a membrane fusion protein of the Lip system, could secrete an anti-green fluorescent protein (GFP)-Nb, a nanobody against GFP, without the addition of a long amino acid sequence. The LipC-based secretion system recognized the Val-Thr-Val sequence at the C-terminus of the nanobody. Finally, Strep-tagged anti-GFP-Nb was purified from culture supernatants of E. coli harboring LipC by Strep-affinity chromatography at a final yield of >5 mg per liter of culture supernatant. These results potently supported that the S. marcescens LipC-based secretion system has the potential to establish an efficient secretory production system for nanobodies.
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http://dx.doi.org/10.1016/j.bbrc.2021.02.096DOI Listing
April 2021

Calcified Aortic Wall Removal for Dysphagia Aortica Caused by Chronic Traumatic Aortic Pseudoaneurysm.

Ann Vasc Surg 2021 Feb 5. Epub 2021 Feb 5.

Department of Cardiovascular surgery, Akita University Graduate School of Medicine, Akita, Japan.

In this study, we report a case of a 45-year-old man with dysphagia aortica secondary to chronic traumatic aortic pseudoaneurysm of the aortic isthmus. He had been involved in a motor vehicle accident 27 years earlier. Computed tomography demonstrated a severely calcified aortic pseudoaneurysm of the aortic isthmus that compressed the esophagus extrinsically. An invasive surgical procedure involving a graft replacement and removal of the calcified aortic wall released the esophageal compression and completely improved the patient's symptoms. To the best of our knowledge, a case of dysphagia aortica caused by calcified pseudoaneurysm has never been reported.
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http://dx.doi.org/10.1016/j.avsg.2021.01.088DOI Listing
February 2021

Direct Abdominal Aortic Access for Thoracic Endovascular Aortic Repair in a Patient with Severe Aortic and Arterial Calcification.

Ann Vasc Surg 2021 May 15;73:509.e21-509.e24. Epub 2021 Jan 15.

Department of Cardiovascular surgery, Akita University Graduate School of Medicine, Akita, Japan.

Aortic and arterial calcification is a complication of advanced atherosclerosis and is a critical intraoperative issue that can reduce the ability to achieve safe and adequate access for stent graft introduction. Different vascular access sites are used to deliver stent grafts when a standard transfemoral or iliac access is not feasible. We report a challenging case of a direct transabdominal aortic thoracic endovascular aortic repair for a thoracic aortic aneurysm complicated with severe aortic and arterial calcification, in which the noncalcified area of the infrarenal abdominal aorta was extremely limited. This may be a reasonable access site, especially for patients with severe aortic and arterial calcification.
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http://dx.doi.org/10.1016/j.avsg.2020.11.001DOI Listing
May 2021

[Tricuspid Valve Replacement Preserving the Leaflets in a Congenitally Corrected Transposition of the Great Arteries;Report of a Case].

Kyobu Geka 2020 Dec;73(13):1109-1112

Department of Cardiovascular Surgery, Akita University, Akita, Japan.

In patients with a congenitally corrected transposition of the great arteries (ccTGA), the regurgitation of the systemic atrioventricular valve (SAVV) increases and anatomical right ventricular (ARV) dysfunction often progressively develops. A low systemic ventricular ejection fraction( SVEF) is a risk factor for mortality. However, in patients with a low ejection fraction of ARV, it is unclear how to best perform valve replacement for patients with regurgitation of SAVV. A 70-year-old female with respirator discomfort was admitted to our hospital and diagnosed to have situs solitus ccTGA, severe SAVV regurgitation, and ARV dysfunction. Her ARV ejection fraction was 25% and she was therefore dependent on inotropic agents. We successfully performed a tricuspid valve replacement while preserving the leaflets, the chorda tendineae's, and papillary muscles, and placing the lead for cardiac resynchronization therapy on the ARV. Her postoperative course was uneventful. Thereafter, she was discharged 6 weeks after surgery.
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December 2020

[Thoracic Endovascular Repair for Chronic Aortic Dissection with Aberrant Origin of the Left Vertebral Artery;Report of a Case].

Kyobu Geka 2020 Nov;73(12):1032-1036

Department of Cardiovascular Surgery, Akita University, Akita, Japan.

We herein report a case of thoracic endovascular aortic repair( TEVAR) for chronic aortic dissection with an aberrant left vertebral artery( LVA) originating from the aortic arch. A 51-year-old man with a medical history of Stanford type B acute aortic dissection 2 years ago was transferred to our institution for the treatment of an aortic expansion. Computed tomography showed a large entry just distal to the takeoff of the left subclavian artery and a dilated dissected thoracic aorta. A left cervical incision over the anterior border of the sternocleidomastoid was made, and the LVA was identified. The proximal LVA was ligated and anastomosed to the left common carotid artery in an end-to-side fashion. After completion of the carotid-subclavian bypass, TEVAR was performed in the usual fashion. The postoperative course was uneventful without stroke or spinal cord injury. At the 1-year follow-up, the false lumen had shrunk and the LVA remained patent.
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November 2020

Total arch repair with frozen elephant trunk for type A acute aortic dissection: the "zone 0 arch repair" strategy.

Ann Cardiothorac Surg 2020 May;9(3):251-253

Department of Cardiovascular Surgery, Akita University Graduate School of Medicine, Akita, Japan.

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http://dx.doi.org/10.21037/acs.2020.02.16DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7298236PMC
May 2020

Multiple graft injury due to migrated nonbroken sternal wires 2 years after cardiac surgery.

J Card Surg 2020 Jul 2;35(7):1640-1641. Epub 2020 Jun 2.

Department of Cardiovascular Surgery, Akita University Graduate School of Medicine, Akita, Japan.

The migration of sternal wires into vital structures is a rare but potentially life-threatening complication and associated with infection in some cases. While a few cases have been reported the sternal wires were broken in those cases. To our knowledge, this is the first report of multiple, nonbroken, migrated sternal wires stabbing vascular grafts.
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http://dx.doi.org/10.1111/jocs.14685DOI Listing
July 2020

Comparative study of hyperpolarization-activated currents in pulmonary vein cardiomyocytes isolated from rat, guinea pig, and rabbit.

J Physiol Sci 2020 Feb 11;70(1). Epub 2020 Feb 11.

Department of Cell Physiology, Akita University Graduate School of Medicine, Hondo 1-1-1, Akita, 010-8543, Japan.

Pulmonary vein (PV) cardiomyocytes have the potential to generate spontaneous activity, in contrast to working myocytes of atria. Different electrophysiological properties underlie the potential automaticity of PV cardiomyocytes, one being the hyperpolarization-activated inward current (I), which facilitates the slow diastolic depolarization. In the present study, we examined pharmacological characteristics of the I of PV cardiomyocytes in rat, guinea pig and rabbit. The results showed that guinea pig and rat PV cardiomyocytes possessed sizeable amplitudes of the I, and the I of guinea pig was suppressed by Cs, a blocker of the hyperpolarization-activated cation current. However, the I of rat was not suppressed by Cs, but by Cd, a blocker of the Cl current. The current density of the I of rabbit PV cardiomyocytes was significantly smaller than those of other species. This suggests that the ion channels that carry the I of PV cardiomyocytes differ among the animal species.
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http://dx.doi.org/10.1186/s12576-020-00736-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7012960PMC
February 2020

Molecular identification of HSPA8 as an accessory protein of a hyperpolarization-activated chloride channel from rat pulmonary vein cardiomyocytes.

J Biol Chem 2019 11 10;294(44):16049-16061. Epub 2019 Sep 10.

Department of Cell Physiology, Akita Graduate School of Medicine, 1-1-1 Hondo, Akita 010-8543, Japan

Pulmonary veins (PVs) are the major origin of atrial fibrillation. Recently, we recorded hyperpolarization-activated Cl current () in rat PV cardiomyocytes. Unlike the well-known chloride channel protein 2 (CLCN2) current, the activation curve of was hyperpolarized as the Cl ion concentration ([Cl] ) increased. This current could account for spontaneous activity in PV cardiomyocytes linked to atrial fibrillation. In this study, we aimed to identify the channel underlying Using RT-PCR amplification specific for or its homologs, a chloride channel was cloned from rat PV and detected in rat PV cardiomyocytes using immunocytochemistry. The gene sequence and electrophysiological functions of the protein were identical to those previously reported for , with protein activity observed as a hyperpolarization-activated current by the patch-clamp method. However, the [Cl] dependence of activation was entirely different from the observed of PV cardiomyocytes; the activation curve of the -transfected cells shifted toward positive potential with increased [Cl] , whereas the of PV and left ventricular cardiomyocytes showed a leftward shift. Therefore, we used MS to explore the possibility of additional proteins interacting with CLCN2 and identified an individual 71-kDa protein, HSPA8, that was strongly expressed in rat PV cardiomyocytes. With co-expression of HSPA8 in HEK293 and PC12 cells, the CLCN2 current showed voltage-dependent activation and shifted to negative potential with increasing [Cl] Molecular docking simulations further support an interaction between CLCN2 and HSPA8. These findings suggest that CLCN2 in rat heart contains HSPA8 as a unique accessory protein.
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http://dx.doi.org/10.1074/jbc.RA119.007416DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6827309PMC
November 2019

Combined effects of obesity and objectively-measured daily physical activity on the risk of hypertension in middle-aged Japanese men: A 4-year prospective cohort study.

Obes Res Clin Pract 2019 Jul - Aug;13(4):365-370. Epub 2019 May 7.

Department of Health Sciences, Nagoya University Graduate School of Medicine, 1-1-20, Daiko-minami, Higashi-ku, Nagoya 461-8673, Japan. Electronic address:

Background: The combined effects of physical inactivity and obesity on hypertension have been recognized; however, previous studies evaluated physical activity using questionnaires. We aimed to examine the effects of physical activity, measured using an accelerometer, and obesity on hypertension onset.

Methods: At baseline, 426 middle-aged Japanese men who were not on antihypertensive medications were included. Physical activity was measured for 7 consecutive days using an accelerometer. Mean daily moderate to vigorous physical activity (MVPA) and step count (SC) were calculated. Low MVPA and low SC were each defined as the first tertile. Obesity was defined as ≥25 kg/m2 of body mass index. The onset of hypertension was defined as receiving antihypertensive agents during the 4-year follow-up. The combined effects of obesity and physical inactivity on hypertension were examined using Cox regression analysis. Potential confounders included age, smoking, alcohol consumption, daily salt intake, dyslipidemia, diabetes mellitus, and systolic and diastolic blood pressures.

Results: Cox regression analysis revealed that both obesity and low MVPA predicted hypertension in patients, independent of confounders (hazard ratio [HR]: 2.64, 95% confidence interval [CI]: 1.08-6.42, p = 0.033), unlike obesity alone (HR: 1.50, 95% CI: 0.50-3.26, p = 0.590). Stratification by obesity and SC revealed similar hypertension risks among the two groups (Obesity with low SC [HR: 2.10, 95% CI 0.88-5.24, p = 0.089]; Obesity without low SC [HR: 1.72, 95% CI 0.93-4.01, p = 0.082]).

Conclusions: Here, findings suggest that the coexistence of obesity and decreased MVPA may increase the risk of hypertension onset.
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http://dx.doi.org/10.1016/j.orcp.2019.04.002DOI Listing
June 2020

Total arch repair with frozen elephant trunk using the "zone 0 arch repair" strategy for type A acute aortic dissection.

J Thorac Cardiovasc Surg 2019 Feb 15. Epub 2019 Feb 15.

Department of Cardiovascular Surgery, Akita University Graduate School of Medicine, Akita, Japan.

Objective: The aim of this study was to investigate the effect of frozen elephant trunk deployment from the zone 0 aorta to the descending aorta on early and midterm postoperative results in patients with acute type A aortic dissection.

Methods: Between October 2014 and April 2018, 108 patients underwent a combined strategy of frozen elephant trunk deployment, ascending aortic replacement, and arch vessel reconstruction ("zone 0 arch repair" strategy) for acute type A aortic dissection (excluding DeBakey type II). Of the 108 patients, 32 (29.6%) had primary tears of the aortic arch or descending aorta.

Results: The 30-day mortality rate was 2.8% (3 patients), and in-hospital mortality rate was 6.5% (7 patients). New-onset permanent neurologic dysfunction and spinal cord injury occurred in 3.7% and 0% of patients, respectively. Five of the 101 survivors underwent thoracic endovascular aortic repair during hospitalization (2 for rapid false lumen enlargement; 3 for true lumen stenosis). The overall survival was 89.8%, 88.1%, and 88.1% at 1, 2, and 3 years, respectively. The cumulative incidence of distal aortic reintervention was 5.8%, 9.1%, and 9.1% at 1, 2, and 3 years, respectively. Two patients underwent thoracic endovascular aortic repair for distal aortic enlargement after discharge.

Conclusions: The use of the "zone 0 arch repair" strategy can eliminate the need for invasive aortic arch resection. It also eliminates the false lumen and produces satisfactory early and midterm postoperative results. Therefore, it can be an alternative to hemiarch and total arch replacements, which are based on a conventional "tear-oriented resection" strategy.
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http://dx.doi.org/10.1016/j.jtcvs.2019.01.125DOI Listing
February 2019

[In situ Replacement with Bovine Pericardial Roll Graft for Infected Aneurysm of the Thoracoabdominal Aorta].

Kyobu Geka 2015 Nov;68(12):976-9

Department of Cardiovascular Surgery, Akita University, Akita, Japan.

A 63-year-old man presented to a nearby doctor with fever and lumbago. Enhanced computed tomography showed a thoracoabdominal aortic aneurysm and enhancement of soft tissue around the aneurysm. He was diagnosed with an infected thoracoabdominal aortic aneurysm and given antibiotics. After 2 weeks, the infection was controlled and he underwent in situ replacement with a bovine pericardial roll graft. A week after the operation, the inflammatory reaction was increased, but the bovine pericardial roll graft was not infected. This suggests that a bovine pericardial roll graft is a suitable material for use in patients with bacterial infections.
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November 2015

A new role for RINT-1 in SNARE complex assembly at the trans-Golgi network in coordination with the COG complex.

Mol Biol Cell 2013 Sep 24;24(18):2907-17. Epub 2013 Jul 24.

School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo 192-0392, Japan Division of Oral Biochemistry, Niigata University Graduate School of Medical and Dental Sciences, 2-5274 Gakkocho-dori, Chuo-ku, Niigata 951-8514, Japan Department of Cell Biology, Fukuoka University School of Medicine, Jonan-ku, Fukuoka 814-0180, Japan.

Docking and fusion of transport vesicles/carriers with the target membrane involve a tethering factor-mediated initial contact followed by soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE)-catalyzed membrane fusion. The multisubunit tethering CATCHR family complexes (Dsl1, COG, exocyst, and GARP complexes) share very low sequence homology among subunits despite likely evolving from a common ancestor and participate in fundamentally different membrane trafficking pathways. Yeast Tip20, as a subunit of the Dsl1 complex, has been implicated in retrograde transport from the Golgi apparatus to the endoplasmic reticulum. Our previous study showed that RINT-1, the mammalian counterpart of yeast Tip20, mediates the association of ZW10 (mammalian Dsl1) with endoplasmic reticulum-localized SNARE proteins. In the present study, we show that RINT-1 is also required for endosome-to-trans-Golgi network trafficking. RINT-1 uncomplexed with ZW10 interacts with the COG complex, another member of the CATCHR family complex, and regulates SNARE complex assembly at the trans-Golgi network. This additional role for RINT-1 may in part reflect adaptation to the demand for more diverse transport routes from endosomes to the trans-Golgi network in mammals compared with those in a unicellular organism, yeast. The present findings highlight a new role of RINT-1 in coordination with the COG complex.
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http://dx.doi.org/10.1091/mbc.E13-01-0014DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3771952PMC
September 2013

Rice (Oryza sativa L.) roots have iodate reduction activity in response to iodine.

Front Plant Sci 2013 10;4:227. Epub 2013 Jul 10.

Laboratory of Plant Nutrition, Department of Plant Science, United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology Utsunomiya, Japan.

Although iodine is not an essential nutrient for higher plants, their roots take up and transport the element. However, the exact mechanisms involved in iodine uptake and metabolism in higher plants have yet to be elucidated. In this study, we compared two cultivars differing in iodine tolerance ("Nipponbare" and "Gohyakumangoku") to increasing levels of I(-) and IO(-) 3 in the root solutions of water-cultured rice (Oryza sativa L.). We found that IO(-) 3 added to the root solutions was converted to I(-) in the presence of roots. Iodate reduction occurred over the course of several hours. Furthermore, the iodate reduction activity of "Nipponbare" (iodine-sensitive) and "Gohyakumangoku" (iodine-tolerant) roots increased after adding IO(-) 3 or I(-). The roots of barley and soybean also showed iodate reduction activity and the activity responded to iodine treatment either with IO(-) 3 and I(-). This study suggests that plant roots biologically reduce iodate to iodide and indicates that the iodate reduction activity of roots responds to external iodine conditions.
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http://dx.doi.org/10.3389/fpls.2013.00227DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3706741PMC
July 2013