Publications by authors named "Da Wang"

314 Publications

Full-Length Genome Sequencing of SARS-CoV-2 Directly from Clinical and Environmental Samples Based on the Multiplex Polymerase Chain Reaction Method.

Biomed Environ Sci 2021 Sep;34(9):725-728

National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.

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http://dx.doi.org/10.3967/bes2021.100DOI Listing
September 2021

Reducing autocorrelation time in determinant quantum Monte Carlo using the Wang-Landau algorithm: Application to the Holstein model.

Phys Rev E 2021 Aug;104(2-2):025305

National Laboratory of Solid State Microstructures & School of Physics, Nanjing University, Nanjing, 210093, China.

When performing a Monte Carlo calculation, the running time should, in principle, be much longer than the autocorrelation time in order to get reliable results. Among different lattice fermion models, the Holstein model is notorious for its particularly long autocorrelation time. In this paper, we employ the Wang-Landau algorithm in the determinant quantum Monte Carlo to achieve the flat-histogram sampling in the "configuration weight space," which can greatly reduce the autocorrelation time by sacrificing some sampling efficiency. The proposal is checked in the Holstein model on both square and honeycomb lattices. Based on such a Wang-Landau assisted determinant quantum Monte Carlo method, some models with long autocorrelation times can now be simulated possibly.
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http://dx.doi.org/10.1103/PhysRevE.104.025305DOI Listing
August 2021

Factors Prognostic for Brain Metastases from Colorectal Cancer: A Single-Center Experience in China.

Cancer Manag Res 2021 29;13:6767-6774. Epub 2021 Aug 29.

Department of Colorectal Surgery and Oncology, Key Laboratory of Cancer Prevention and Intervention, Ministry of Education, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang Province, People's Republic of China.

Purpose: This study aimed to analyze clinicopathological, survival, prognostic factors, as well as the timing of brain metastases (BM) in colorectal cancer (CRC) using data from a Chinese center.

Patients And Methods: Data of 65 consecutive CRC patients with BM were collected from a single institution in China. The time from primary tumor surgery to the occurrence of BM was calculated. Kaplan-Meier analysis was used to evaluate cumulative survival of patients. Factors associated with prognosis of overall survival (OS) were explored using Cox's proportional hazard regression models.

Results: The median time interval from CRC surgery to the diagnosis of BM was 24 months. After diagnosis of BM, median OS values for patients were 11 months. Extracranial metastases occurred in 45 cases (69.2%) when BM was diagnosed, and 58.5% of these patients with lung metastases Time of BMs (=0.018), presence of extracranial metastases (=0.033), treatment (=0.003), CA199 (=0.034), CA125 (<0.001), CA242 (=0.018), and CA211 (=0.012) were associated with OS of patients through univariate analysis. Multivariate analysis using a Cox regression model showed that only treatment was an independent predictor for OS (conservative treatment; HR=1.861, 95% CI=1.077-3.441; =0.048).

Conclusion: Surgical treatment of metastatic lesions may be an alternative choice for CRC patients with BM. Identifying the timing of brain metastases can help to detect this disease early, leading to a better survival outcome.
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http://dx.doi.org/10.2147/CMAR.S320179DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8412826PMC
August 2021

Study and Experimental Validation of the Functional Components and Mechanisms of Baroni in the Treatment of Lactation Deficiency.

Foods 2021 Aug 12;10(8). Epub 2021 Aug 12.

College of Food Science, South China Agricultural University, Guangzhou 510642, China.

The function of Baroni (daylily) on promoting lactation is reported in several ancient Chinese medicine books. However, nowadays, there is no conclusive data to support this statement. In this study, we investigated the effect of Baroni extract (HCE) on lactation insufficiency in chronic unpredictable mild stress (CUMS) dams and further explored the mechanism and functional components through network pharmacology. The results showed that HCE could increase the offspring's weight, serum prolactin (PRL), and oxytocin (OT) level of CUMS dams. Network pharmacology analysis revealed that the facilitation of HCE on lactation is the result of the comprehensive action of 62 components on 209 targets and 260 pathways, among this network, quercetin, kaempferol, thymidine, etc., were the vital material basis, signal transducer and activator of transcription 3 (STAT3), mitogen activity protein kinase 1 (MAPK1), tumor protein P53 (TP53), etc., were the core targets, and the prolactin signaling pathway was the core pathway. In addition, verification test results showed that HCE regulated the abnormal expression of the prolactin signaling pathway, including STAT3, cyclin D1 (CCND1), MAPK1, MAPK8, nuclear factor NF-kappa-B p105 subunit (NFKB1), and tyrosine-protein kinase (JAK2). In conclusion, HCE exhibited a facilitation of lactation insufficiency, in which quercetin, kaempferol, thymidine, etc., were the most important material basis. The mechanism of this promotional effect is mediated by the prolactin signaling pathway in mammary gland.
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http://dx.doi.org/10.3390/foods10081863DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8391212PMC
August 2021

Prognostic factors for ovarian metastases in colorectal cancer patients.

World J Surg Oncol 2021 Jul 20;19(1):220. Epub 2021 Jul 20.

Department of Colorectal Surgery and Oncology, Key Laboratory of Cancer Prevention and Intervention, Ministry of Education, the Second Affiliated Hospital, Zhejiang University School of Medicine, 88 Jiefang Road, Hangzhou, Zhejiang Province, 310009, People's Republic of China.

Purpose: The aim of this study was to analyze prognostic factors for ovarian metastases (OM) in colorectal cancer (CRC) using data from a Chinese center. In addition, the study aimed at developing a new clinical scoring system for prognosis of OM of CRC patients after surgery.

Patients And Methods: Data of CRC patients with OM were collected from a single Chinese institution (n = 67). Kaplan-Meier analysis was used to evaluate cumulative survival of patients. Factors associated with prognosis of overall survival (OS) were explored using Cox's proportional hazard regression models. A scoring system to determine effectiveness of prognosis was developed.

Results: Median OS values for patients with or without surgery were 22 and 7 months, respectively. Size of OM, number of OM, peritoneal metastasis (PM), Peritoneal cancer index (PCI), and completeness of cytoreduction (CC) were associated with OS of patients through univariate analysis. Multivariate analysis using a Cox regression model showed that only CC was an independent predictor for OS. Three variables (the size of OM >15cm, PCI ≥ 10, and carcinoembryonic antigen (CEA) >30 ng/mL) assigned one point each were used to develop a risk score. The resulting score was used for prognosis of OS.

Conclusion: Surgical treatment of metastatic sites is effective and safe for CRC patients with OM. CC-0 is recommended for improved prognosis. The scoring system developed in this study is effective for prediction of OS of patients after surgery.
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http://dx.doi.org/10.1186/s12957-021-02305-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8293531PMC
July 2021

Facile treatment tuning the morphology of Pb with state-of-the-art selectivity in CO electroreduction to formate.

Chem Commun (Camb) 2021 Jul;57(60):7418-7421

Key Laboratory of Microbial Technology for Industrial Pollution Control of ZhejiangProvince, College of Environment, Zhejiang University of Technology, Hangzhou 310032, People's Republic of China.

This study presents a facile treatment to modify the commercial irregular shaped polycrystalline Pb into well-defined octahedral Pb with unique Pb(111) facets. Efficient, selective, and stable electrochemical reduction of CO2 toward formate has been achieved on the treated Pb electrode. The faradaic efficiency of formate production from the CO2RR is 98.03%, which is the highest reported to date. The results from the combination of theoretical calculations and experimental tests demonstrate that the enhanced catalytic performance on the treated Pb electrode stems from the electrode morphology characterized by a unique Pb(111) surface with lower Gibbs free energies (ΔG) for the formation of intermediate OCHO*.
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http://dx.doi.org/10.1039/d1cc02113cDOI Listing
July 2021

[Simultaneous determination of amino acid and monoamine neurotransmitters in serum by high performance liquid chromatography coupled with precolumn derivatization].

Se Pu 2020 Aug;38(8):923-928

Medical Comprehensive Experimental Center, Hebei University, Baoding 071000, China.

Using -phthalaldehyde (OPA) as the derivatization reagent, a precolumn derivatized -high performance liquid chromatography (HPLC) method was developed for the simultaneous determination of amino acid neurotransmitters taurine (Tau), glutamic acid (Glu), glycine(Gly), and γ -aminobutyric acid (γ-GABA), as well as the monoamine neurotransmitter dopamine (DA), in serum samples. The samples and ethanol were mixed at a volume ratio of 1:2 (v/v) for protein precipitation. After centrifugation, the supernatant was withdrawn and blown to dryness using nitrogen. The residue was pre-column derivatized with OPA, and the derivatized product was isolated by gradient elution ona Luna 5u C18 column (250 mm×4.6 mm, 5 μm). Under the optimal experimental conditions, the five neurotransmitters showed good linearities ( ≥ 0.9866). The limits of detection were between 0.10 and 0.40 μmol/L. The spiked recoveries at different spiked levels were 87.57%-115.31%, and the RSDs were below 7.80%. This method is simple, sensitive, and it can be promised for the simultaneous detection of amino acid and monoamine neurotransmitters.
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http://dx.doi.org/10.3724/SP.J.1123.2019.12029DOI Listing
August 2020

Efficient long-range conduction in cable bacteria through nickel protein wires.

Nat Commun 2021 06 28;12(1):3996. Epub 2021 Jun 28.

IMEC, Leuven, Belgium.

Filamentous cable bacteria display long-range electron transport, generating electrical currents over centimeter distances through a highly ordered network of fibers embedded in their cell envelope. The conductivity of these periplasmic wires is exceptionally high for a biological material, but their chemical structure and underlying electron transport mechanism remain unresolved. Here, we combine high-resolution microscopy, spectroscopy, and chemical imaging on individual cable bacterium filaments to demonstrate that the periplasmic wires consist of a conductive protein core surrounded by an insulating protein shell layer. The core proteins contain a sulfur-ligated nickel cofactor, and conductivity decreases when nickel is oxidized or selectively removed. The involvement of nickel as the active metal in biological conduction is remarkable, and suggests a hitherto unknown form of electron transport that enables efficient conduction in centimeter-long protein structures.
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http://dx.doi.org/10.1038/s41467-021-24312-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8238962PMC
June 2021

Quantitative 3D real-space analysis of Laves phase supraparticles.

Nat Commun 2021 Jun 25;12(1):3980. Epub 2021 Jun 25.

Soft Condensed Matter, Debye Institute for Nanomaterials Science, Utrecht University, Utrecht, The Netherlands.

Assembling binary mixtures of nanoparticles into crystals, gives rise to collective properties depending on the crystal structure and the individual properties of both species. However, quantitative 3D real-space analysis of binary colloidal crystals with a thickness of more than 10 layers of particles has rarely been performed. Here we demonstrate that an excess of one species in the binary nanoparticle mixture suppresses the formation of icosahedral order in the self-assembly in droplets, allowing the study of bulk-like binary crystal structures with a spherical morphology also called supraparticles. As example of the approach, we show single-particle level analysis of over 50 layers of Laves phase binary crystals of hard-sphere-like nanoparticles using electron tomography. We observe a crystalline lattice composed of a random mixture of the Laves phases. The number ratio of the binary species in the crystal lattice matches that of a perfect Laves crystal. Our methodology can be applied to study the structure of a broad range of binary crystals, giving insights into the structure formation mechanisms and structure-property relations of nanomaterials.
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http://dx.doi.org/10.1038/s41467-021-24227-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8233429PMC
June 2021

Orthorhombic Cobalt Ditelluride with Te Vacancy Defects Anchoring on Elastic MXene Enables Efficient Potassium-Ion Storage.

Adv Mater 2021 Aug 24;33(31):e2100272. Epub 2021 Jun 24.

School of Materials Science and Engineering, Peking University, Beijing, 100871, China.

The fast and reversible potassiation/depotassiation of anode materials remains an elusive yet intriguing goal. Herein, a class of the P-doping-induced orthorhombic CoTe nanowires with Te vacancy defects supported on MXene (o-P-CoTe /MXene) is designed and prepared, taking advantage of the synergistic effects of the conductive o-P-CoTe arrays with rich Te vacancy defects and the elastic MXene sheets with self-autoadjustable function. Consequently, the o-P-CoTe /MXene superstructure exhibits boosted potassium-storage performance, in terms of high reversible capacity (373.7 mAh g at 0.2 A g after 200 cycles), remarkable rate capability (168.2 mAh g at 20 A g ), and outstanding long-term cyclability (0.011% capacity decay per cycle over 2000 cycles at 2 A g ), representing the best performance in transition-metal-dichalcogenides-based anodes to date. Impressively, the flexible full battery with o-P-CoTe /MXene anode achieves a satisfying energy density of 275 Wh kg and high bending stability. The kinetics analysis and first-principles calculations reveal superior pseudocapacitive property, high electronic conductivity, and favorable K ion adsorption and diffusion capability, corroborating fast K ion storage. Especially, ex situ characterizations confirm o-P-CoTe /MXene undergoes reversible evolutions of initially proceeding with the K ion insertion, followed by the conversion reaction mechanism.
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http://dx.doi.org/10.1002/adma.202100272DOI Listing
August 2021

HKUST-1-derived highly ordered Cu nanosheets with enriched edge sites, stepped (211) surfaces and (200) facets for effective electrochemical CO reduction.

Chemosphere 2021 Sep 29;278:130408. Epub 2021 Mar 29.

College of Environment, Zhejiang University of Technology, Hangzhou, 310032, China. Electronic address:

A novel electrode composed of Cu nanosheets constructed from nanoparticles was synthesized by in situ electrochemical derivation from the metal-organic framework (MOF) HKUST-1. The prepared derivative electrode (HE-Cu) exhibited higher Faradaic efficiency (FE, 56.0%) of electrochemical CO reduction (COR) compared with that of pristine Cu foil (p-Cu, 32.3%) at an overpotential of -1.03 V vs. a reversible hydrogen electrode (RHE). HE-Cu also exhibited lower onset potential of COR as well as inhibiting the H evolution reaction. Electrochemical measurements revealed that HE-Cu exhibited higher CO adsorption (1.58-fold) and a larger electrochemical active surface area (1.24-fold) compared with p-Cu. Physicochemical characterization and Tafel analysis showed that stepped Cu (211) surfaces, (200) facets and Cu edge atoms on HE-Cu contributed significantly to the enhanced COR activity and/or HCOOH and/or C2 product selectivity. The FEs of HCOOH and C2 products for HE-Cu increased 1.57-fold and 10.6-fold at an overpotential of -1.19 V vs. RHE compared with p-Cu. Although CH was produced on p-Cu, its formation was totally suppressed on HE-Cu due to the increase of edge sites and (200) facets. Our study demonstrates that electroreduction of MOFs is a promising method to prepare novel and stable electrochemical catalysts with unique surface structures. The fabricated derivative electrode not only promoted electrochemical COR activity but also exhibited high C2 product selectivity.
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http://dx.doi.org/10.1016/j.chemosphere.2021.130408DOI Listing
September 2021

Slice encoding for the reduction of outflow signal artifacts in cine balanced SSFP imaging.

Magn Reson Med 2021 10 31;86(4):2034-2048. Epub 2021 May 31.

Department of Radiological Sciences, University of California Los Angeles, Los Angeles, California, USA.

Purpose: Standard balanced SSFP (bSSFP) cine MRI often suffers from blood outflow artifacts. We propose a method that spatially encodes these outflowing spins to reduce their effects in the intended slice.

Methods: Bloch simulations were performed to characterize through-plane flow and to investigate how the use of phase encoding along the slice select's direction ("slice encoding") could alleviate its issues. Phantom scans and in vivo cines were acquired on a 3T system, comparing the standard 2D acquisition to the proposed slice-encoding method. Nineteen healthy volunteers were recruited for short-axis and horizontal long-axis oriented scans. An expert radiologist evaluated each slice-encoded/standard cine pairs in a rank comparison test and graded their quality on a 1-5 scale. The grades were used for a nonparametric paired evaluation for independent samples with a null hypothesis that there was no statistical difference between the two quality-grade distributions for α = 0.05 significance.

Results: Bloch simulation results demonstrated this technique's feasibility, showing a fully resolved slice profile given a sufficient number of slice encodes. These results were confirmed with the phantom experiments. Each in vivo slice-encoded cine had a higher quality than its corresponding standard acquisition. The nonparametric paired evaluation came to 0.01 significance, encouraging us to reject the null hypothesis and conclude that slice-encoding effectively works in reducing outflow effects.

Conclusion: The slice-encoding balanced SSFP technique is helpful in mitigating outflow effects and is achievable within a single breath hold, being a useful alternative for cases in which the flow artifacts are significant.
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http://dx.doi.org/10.1002/mrm.28858DOI Listing
October 2021

Postoperative circulating tumor DNA as markers of recurrence risk in stages II to III colorectal cancer.

J Hematol Oncol 2021 05 17;14(1):80. Epub 2021 May 17.

Department of Medical Oncology, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-Sen University Cancer Center, 651 Dongfeng Road East, Guangzhou, 510060, China.

Background: Precise methods for postoperative risk stratification to guide the administration of adjuvant chemotherapy (ACT) in localized colorectal cancer (CRC) are still lacking. Here, we conducted a prospective, observational, and multicenter study to investigate the utility of circulating tumor DNA (ctDNA) in predicting the recurrence risk.

Methods: From September 2017 to March 2020, 276 patients with stage II/III CRC were prospectively recruited in this study and 240 evaluable patients were retained for analysis, of which 1290 serial plasma samples were collected. Somatic variants in both the primary tumor and plasma were detected via a targeted sequencing panel of 425 cancer-related genes. Patients were treated and followed up per standard of care.

Results: Preoperatively, ctDNA was detectable in 154 of 240 patients (64.2%). At day 3-7 postoperation, ctDNA positivity was associated with remarkably high recurrence risk (hazard ratio [HR], 10.98; 95%CI, 5.31-22.72; P < 0.001). ctDNA clearance and recurrence-free status was achieved in 5 out of 17 ctDNA-positive patients who were subjected to ACT. Likewise, at the first sampling point after ACT, ctDNA-positive patients were 12 times more likely to experience recurrence (HR, 12.76; 95%CI, 5.39-30.19; P < 0.001). During surveillance after definitive therapy, ctDNA positivity was also associated with extremely high recurrence risk (HR, 32.02; 95%CI, 10.79-95.08; P < 0.001). In all multivariate analyses, ctDNA positivity remained the most significant and independent predictor of recurrence-free survival after adjusting for known clinicopathological risk factors. Serial ctDNA analyses identified recurrence with an overall accuracy of 92.0% and could detect disease recurrence ahead of radiological imaging with a mean lead time of 5.01 months.

Conclusions: Postoperative serial ctDNA detection predicted high relapse risk and identified disease recurrence ahead of radiological imaging in patients with stage II/III CRC. ctDNA may be used to guide the decision-making in postsurgical management.
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http://dx.doi.org/10.1186/s13045-021-01089-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8130394PMC
May 2021

Prognostic factors and survival prediction of pediatric glioblastomas: a population-based study.

Turk Neurosurg 2020 Oct 16. Epub 2020 Oct 16.

Hwa Mei Hospital, University of Chinese Academy of Sciences, Department of Emergency Medicine, Zhejiang Province, China.

Aim: Pediatric glioblastoma (GBM) is extremely rare and malignant. Clinical characteristics and prognostic factors of pediatric GBM remain unclear. Our study aimed to identify risk factors of this disease, and develop an effective prediction model to estimate survival rate for these patients.

Material And Methods: The data of pediatric patients with GBM were extracted from the Surveillance, Epidemiology, and End Results (SEER) database. The Kaplan-Meier analyses were performed for overall survival. Significant prognostic factors were identified by univariate and multivariate Cox regression. The nomogram model was also established.

Results: A total of 378 pediatric patients with GBM were included in our study. Multivariate Cox analysis revealed that age at diagnosis (HR, 1.67; 95% CI, 1.19-2.35; P= 0.003), tumor site (infratentorial vs. supratentorial: HR, 1.44; 95% CI, 1.03-2.03; P = 0.035), surgery (gross total resection vs. no surgery: HR, 0.53; 95% CI, 0.36-0.77, P 0.001) and chemotherapy (HR, 0.56; 95% CI, 0.42-0.74; P 0.001) were independent prognostic factors of overall survival for pediatric GBMs. Additionally, we found patients with tumors located in infratentorial (P 0.001) tended to receive conservative treatment. Moreover, our nomogram model showed favorable discriminative ability.

Conclusion: At the population-level, we found older children and tumors located in infratentorial were associated with poor survival, while both GTR and chemotherapy were associated with improved survival. And there was no association between radiotherapy and survival outcomes. Moreover, a nomogram with good performance was constructed to predict overall survival for these patients.
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http://dx.doi.org/10.5137/1019-5149.JTN.31915-20.2DOI Listing
October 2020

sp. nov., the fifth species of -group from China (Araneae: Clubionidae).

Biodivers Data J 2021 29;9:e66260. Epub 2021 Apr 29.

Hubei Key Laboratory of Radiation Chemistry and Functional Materials, School of Nuclear Technology and Chemistry & Biology, Hubei University of Science and Technology, Xianning, China Hubei Key Laboratory of Radiation Chemistry and Functional Materials, School of Nuclear Technology and Chemistry & Biology, Hubei University of Science and Technology Xianning China.

Background: The -group is a relatively small species group, distributed exclusively in East Asia, with only three species clearly documented so far.

New Information: Dong & Zhang, 2016, which was previously placed in the -group, is assigned to the -group in the present paper. A new spider of the -group from Jiugong Mountain in China is described under the name of sp. nov. Detailed descriptions and photographs of the new species are provided.
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http://dx.doi.org/10.3897/BDJ.9.e66260DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8102465PMC
April 2021

DSCAM/PAK1 pathway suppression reverses neurogenesis deficits in iPSC-derived cerebral organoids from patients with Down syndrome.

J Clin Invest 2021 Jun;131(12)

Department of Stem Cell and Neural Regeneration, State Key Laboratory of Reproductive Medicine, School of Pharmacy, and.

Down syndrome (DS), caused by trisomy of chromosome 21, occurs in 1 of every 800 live births. Early defects in cortical development likely account for the cognitive impairments in DS, although the underlying molecular mechanism remains elusive. Here, we performed histological assays and unbiased single-cell RNA-Seq (scRNA-Seq) analysis on cerebral organoids derived from 4 euploid cell lines and from induced pluripotent stem cells (iPSCs) from 3 individuals with trisomy 21 to explore cell-type-specific abnormalities associated with DS during early brain development. We found that neurogenesis was significantly affected, given the diminished proliferation and decreased expression of layer II and IV markers in cortical neurons in the subcortical regions; this may have been responsible for the reduced size of the organoids. Furthermore, suppression of the DSCAM/PAK1 pathway, which showed enhanced activity in DS, using CRISPR/Cas9, CRISPR interference (CRISPRi), or small-molecule inhibitor treatment reversed abnormal neurogenesis, thereby increasing the size of organoids derived from DS iPSCs. Our study demonstrates that 3D cortical organoids developed in vitro are a valuable model of DS and provide a direct link between dysregulation of the DSCAM/PAK1 pathway and developmental brain defects in DS.
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http://dx.doi.org/10.1172/JCI135763DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8203468PMC
June 2021

Blocking polymerization of CTFs induces plentiful structural terminations for synchronous removal of organics and Cr(VI).

Chem Commun (Camb) 2021 May;57(40):4946-4949

Key Laboratory of Microbial Technology for Industrial Pollution Control of Zhejiang Province, College of Environment, Zhejiang University of Technology, Hangzhou, Zhejiang 310032, P. R. China.

Herein, an ultramild block polymerization strategy was employed to precisely control the exposure of structural terminations in the skeleton of covalent triazine-based frameworks (CTFs). The generated structural terminations with cyano (-CN) and hydroxy (-OH) groups (STCHs) could serve as not only the optimal adsorption sites for enriching targets, but also π-conjugated electron donor-acceptor dyads to accelerate the charge transfer. With spatial separation of charge localization sites, STCH-CTF exhibited a photoactivity of 2.5-4 times higher than that of pristine CTFs in the simultaneous oxidation of bisphenol A (BPA) and the reduction of hexavalent chromium (Cr(vi)).
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http://dx.doi.org/10.1039/d1cc00780gDOI Listing
May 2021

An Insight of the First Community Infected COVID-19 Patient in Beijing by Imported Case: Role of Deep Learning-Assisted CT Diagnosis.

Chin Med Sci J 2021 Mar;36(1):66-71

Department of Infection, Beijing Haidian Section of Peking University Third Hospital (Beijing Haidian Hospital), Beijing 100080, China.

In the era of coronavirus disease 2019 (COVID-19) pandemic, imported COVID-19 cases pose great challenges to many countries. Chest CT examination is considered to be complementary to nucleic acid test for COVID-19 detection and diagnosis. We report the first community infected COVID-19 patient by an imported case in Beijing, which manifested as nodular lesions on chest CT imaging at the early stage. Deep Learning (DL)-based diagnostic systems quantitatively monitored the progress of pulmonary lesions in 6 days and timely made alert for suspected pneumonia, so that prompt medical isolation was taken. The patient was confirmed as COVID-19 case after nucleic acid test, for which the community transmission was prevented timely. The roles of DL-assisted diagnosis in helping radiologists screening suspected COVID cases were discussed.
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http://dx.doi.org/10.24920/003788DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8041135PMC
March 2021

Phenylpropanoid-conjugated pentacyclic triterpenoids from the whole plants of Leptopus lolonum induced cell apoptosis via MAPK and Akt pathways in human hepatocellular carcinoma cells.

Bioorg Chem 2021 06 1;111:104886. Epub 2021 Apr 1.

School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, People's Republic of China; Key Laboratory of Structure-Based Drug Design and Discovery of Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, People's Republic of China. Electronic address:

Our present and previous phytochemical investigations on Leptopus lolonum have resulted in the isolation of almost 30 phenylpropanoid-conjugated pentacyclic triterpenoids (PCPTs). During the continuous study on PCPTs, this kind of triterpenoid ester is considered as a natural product with low toxicity because of it's widely distribution in natural plants and edible fruits including kiwi fruit, durian, jujube, pawpaw, apple and pear. In the present work, we report the isolation, structural elucidation and cytotoxic evaluation of four new PCPTs (1-4) which obtained from L. lolonum. In addition, the possible biosynthesis pathway for 28-norlupane triterpenoid and potent effect of phenylpropanoid moiety for increasing the cytotxic effect of triterpenoids were also discussed. Among these compounds, compound 1 exhibited the highest cytotoxic effect on HepG2 cells with IC value of 11.87 μM. Further flow cytometry and western blot analysis demonstrated that 1 caused G1 cell cycle arrest by up-regulated the expression of phosphorylated p53 protein in HepG2 cells and induced cell apoptosis via MAPK and Akt pathways. These results emphasized the potential of PCPTs as lead compounds for developing anti-cancer drugs.
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http://dx.doi.org/10.1016/j.bioorg.2021.104886DOI Listing
June 2021

Perfluorinated organics regulating LiO formation and improving stability for Li-oxygen batteries.

Chem Commun (Camb) 2021 Mar 24;57(24):3030-3033. Epub 2021 Feb 24.

School of Material Science & Engineering, Shanghai University, Shanghai 200072, P. R. China.

Lithium-oxygen batteries have a high theoretical capacity, but they are still far from meeting the capacity required for practical applications. In this study, we systematically investigate the synergistic effect of perfluorotributylamine (PFTBA) as an additive in a TEGDME-based electrolyte to optimize the electrochemical performance of Li-O batteries. PFTBA promotes cyclic LiO growth, and the discharge capacity is increased to 9548.7 mA h g. Moreover, the hydrophobicity of PFTBA can aid in the protection of the lithium anode against corrosion as it remains stable during cycling. The Li-O battery exhibited enhanced cycling stability (200 cycles) as a consequence. This study reveals that PFTBA increases the capacity and provides more possibilities for the application of perfluorinated chemicals in Li-O batteries.
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http://dx.doi.org/10.1039/d0cc07774gDOI Listing
March 2021

Nitric Oxide-Driven Nanomotor for Deep Tissue Penetration and Multidrug Resistance Reversal in Cancer Therapy.

Adv Sci (Weinh) 2021 Feb 18;8(3):2002525. Epub 2020 Dec 18.

The Comprehensive Cancer Centre of Nanjing Drum Tower Hospital The Affiliated Hospital of Nanjing University Medical School Nanjing 210008 China.

Poor permeation of therapeutic agents and multidrug resistance (MDR) in solid tumors are the two major challenges that lead to the failure of the current chemotherapy methods. Herein, a zero-waste doxorubicin-loaded heparin/folic acid/l-arginine (HFLA-DOX) nanomotor with motion ability and sustained release of nitric oxide (NO) to achieve deep drug penetration and effective reversal of MDR in cancer chemotherapy is designed. The targeted recognition, penetration of blood vessels, intercellular penetration, special intracellular distribution (escaping from lysosomes and accumulating in Golgi and nucleus), 3D multicellular tumor spheroids (3D MTSs) penetration, degradation of tumor extracellular matrix (ECM), and reversal of MDR based on the synergistic effects of the motion ability and sustained NO release performance of the NO-driven nanomotors are investigated in detail. Correspondingly, a new chemotherapy mode called recognition-penetration-reversal-elimination is proposed, whose effectiveness is verified by in vitro cellular experiments and in vivo animal tumor model, which can not only provide effective solutions to these challenges encountered in cancer chemotherapy, but also apply to other therapy methods for the special deep-tissue penetration ability of a therapeutic agent.
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http://dx.doi.org/10.1002/advs.202002525DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7856908PMC
February 2021

The effects of m A modification in central nervous system function and disease.

Yi Chuan 2020 Dec;42(12):1156-1167

Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin 150081, China.

N -methyladenosine (m A) is an important RNA modification, which is highly active in brain tissues, participates in global intracellular mRNA metabolism, and regulates gene expression and a variety of biological processes. Stable m A modification contributes to the normal embryonic brain development and memory formation and plays an important role in maintaining the functions of the central nervous system. However, changes in the level of m A modification and the expression of its related proteins cause abnormal nervous system functions, including brain tissue development retardation, axon regeneration disorders, memory changes, and stem cell renewal and differentiation disorders. Recent studies have also found that m A modification and its related proteins play key roles in the development of various nervous system diseases, such as Alzheimer's disease, Parkinson's disease, fragile X-chromosome syndrome, depression and glioblastoma. In this review, we summarize the research progresses of m A modification regulation mechanism in the central nervous system in recent years, and discusses the effects of gene expression regulation mediated by m A modification on the biological functions of the central nervous system and related diseases, thereby providing some insights on the new research targets and treatment directions for the central nervous system diseases.
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http://dx.doi.org/10.16288/j.yczz.20-233DOI Listing
December 2020

Seeded Growth Combined with Cation Exchange for the Synthesis of Anisotropic Cu S/ZnS, Cu S, and CuInS Nanorods.

Chem Mater 2021 Jan 28;33(1):102-116. Epub 2020 Dec 28.

Debye Institute for Nanomaterials Science, Utrecht University, P.O. Box 80000, 3508 TA Utrecht, The Netherlands.

Colloidal copper(I) sulfide (Cu S) nanocrystals (NCs) have attracted much attention for a wide range of applications because of their unique optoelectronic properties, driving scientists to explore the potential of using Cu S NCs as seeds in the synthesis of heteronanocrystals to achieve new multifunctional materials. Herein, we developed a multistep synthesis strategy toward Cu S/ZnS heteronanorods. The Janus-type Cu S/ZnS heteronanorods are obtained by the injection of hexagonal high-chalcocite Cu S seed NCs in a hot zinc oleate solution in the presence of suitable surfactants, 20 s after the injection of sulfur precursors. The Cu S seed NCs undergo rapid aggregation and coalescence in the first few seconds after the injection, forming larger NCs that act as the effective seeds for heteronucleation and growth of ZnS. The ZnS heteronucleation occurs on a single (100) facet of the Cu S seed NCs and is followed by fast anisotropic growth along a direction that is perpendicular to the -axis, thus leading to Cu S/ZnS Janus-type heteronanorods with a sharp heterointerface. Interestingly, the high-chalcocite crystal structure of the injected Cu S seed NCs is preserved in the Cu S segments of the heteronanorods because of the high-thermodynamic stability of this Cu S phase. The Cu S/ZnS heteronanorods are subsequently converted into single-component Cu S and CuInS nanorods by postsynthetic topotactic cation exchange. This work expands the possibilities for the rational synthesis of colloidal multicomponent heteronanorods by allowing the design principles of postsynthetic heteroepitaxial seeded growth and nanoscale cation exchange to be combined, yielding access to a plethora of multicomponent heteronanorods with diameters in the quantum confinement regime.
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http://dx.doi.org/10.1021/acs.chemmater.0c02817DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7808334PMC
January 2021

Differential effects of arsenic species on Nrf2 and Bach1 nuclear localization in cultured hepatocytes.

Toxicol Appl Pharmacol 2021 02 9;413:115404. Epub 2021 Jan 9.

Environment and Non-Communicable Disease Research Center, Key Laboratory of Arsenic-related Biological Effects and Prevention and Treatment in Liaoning Province, School of Public Health, China Medical University, Shenyang, PR China. Electronic address:

Arsenic is a ubiquitous metalloid element present in both inorganic and organic forms in the environment. The liver is considered to be a primary organ of arsenic biotransformation and methylation, as well as the main target of arsenic toxicity. Studies have confirmed that Chang human hepatocytes have an efficient arsenic methylating capacity. Our previous studies have proven that arsenite activates nuclear factor erythroid 2-related factor 2 (Nrf2) pathway in hepatocytes. This study aimed to explore the activation of the Nrf2 pathway upon treatment of arsenic in various forms, including inorganic and organic arsenic. Our results showed that inorganic arsenic-both AsO and NaHAsO significantly induced the expression of Nrf2 protein and mRNA, enhanced the transcription activity of Nrf2, and induced the expression of downstream target genes. These results confirmed the inorganic arsenic-induced Nrf2 pathway activation in hepatocytes. Although all arsenic chemicals used in the study induced Nrf2 protein accumulation, the organic arsenic CHAsO did not affect the expression of Nrf2 downstream genes which were elevated by inorganic arsenic exposures. Through qRT-PCR and Nrf2 luciferase reporter assays, we further confirmed that CHAsO neither increased Nrf2 mRNA level nor activated the Nrf2 transcription activity. Mechanistically, our results confirmed inorganic arsenic-induced both the nuclear import of Nrf2 and export of Bach1 (BTB and CNC homology 1), which is an Nrf2 transcriptional repressor, while organic arsenic only induced Nrf2 translocation. The unique pattern of Nrf2 regulation by organic arsenic underlines the critical role of Nrf2 and Bach1 in the arsenic toxicology.
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http://dx.doi.org/10.1016/j.taap.2021.115404DOI Listing
February 2021

Construction and prognostic analysis of miRNA-mRNA regulatory network in liver metastasis from colorectal cancer.

World J Surg Oncol 2021 Jan 4;19(1). Epub 2021 Jan 4.

Department of Colorectal Surgery, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou, 310000, Zhejiang, China.

Background: Colorectal cancer (CRC) is one of the most common cancers in the world, and liver metastasis is the leading cause of colorectal cancer-related deaths. However, the mechanism of liver metastasis in CRC has not been clearly elucidated.

Methods: Three datasets from the Gene Expression Omnibus (GEO) database were analyzed to obtain differentially expressed genes (DEGs), which were subjected to functional enrichment analysis and protein-protein interaction analysis. Subsequently, mRNA-miRNA network was constructed, and the associated DEGs and DEMs were performed for prognostic analysis. Finally, we did infiltration analysis of growth arrest specific 1 (GAS1)-associated immune cells.

Results: We obtained 325 DEGs and 9 differentially expressed miRNAs (DEMs) between primary CRC and liver metastases. Enrichment analysis and protein-protein interactions (PPI) further revealed the involvement of DEGs in the formation of the inflammatory microenvironment and epithelial-mesenchymal transition (EMT) during the liver metastases process in CRC. Survival analysis demonstrated that low-expressed GAS1 as well as low-expressed hsa-miR-33b-5p was a favorable prognostic indicator of overall survival. Further exploration of GAS1 revealed that its expression was interrelated with the infiltration of immune cells in tumor tissues.

Conclusions: In summary, DEGs, DEMs, and their interactions found in liver metastasis of CRC may provide a basis for further understanding of the mechanism of CRC metastasis.
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http://dx.doi.org/10.1186/s12957-020-02107-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7784011PMC
January 2021

Molecular Alterations in Metastatic Ovarian Cancer From Gastrointestinal Cancer.

Front Oncol 2020 10;10:605349. Epub 2020 Dec 10.

Department of Colorectal Surgery and Oncology, Key Laboratory of Cancer Prevention and Intervention, Ministry of Education, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Reports indicate that most metastatic ovarian cancer (MOC) originates from gastrointestinal cancer (GIC). Notably, GICs metastasize to the ovary frequently 3 main routes including hematogenous spread, lymphogenous spread, and transcoelomic spread. Nonetheless, the mechanism of the progression remains unknown, and only a handful of literature exists on the molecular alteration implicated in MOC from GIC. This work collected existing evidence and literature on the vital molecules of the metastatic pathway and systematically analyzed them geared toward exploring the mechanism of the metastatic pathway of MOC. Further, this review described dominating molecular alteration in the metastatic process from cancer cells detaching away from lesions to arrive at the ovary, including factors for regulating signaling pathways in epithelial-interstitial transformation, invading, and surviving in the circulatory system or abdominal cavity. We interrogated the basis of the ovary as a distant metastatic site. This article provides new insights into the metastatic pathway and generates novel therapeutic targets for effective treatment and satisfactory outcomes in GIC patients.
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http://dx.doi.org/10.3389/fonc.2020.605349DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7758447PMC
December 2020

A simple method to clean ligand contamination on TEM grids.

Ultramicroscopy 2021 Feb 15;221:113195. Epub 2020 Dec 15.

Electron microscopy for Materials research (EMAT), University of Antwerp, Groenenborgerlaan 171, 2020 Antwerpen, Belgium.

Colloidal nanoparticles (NPs) including nanowires and nanosheets made by chemical methods involve many organic ligands. When the structure of NPs is investigated via transmission electron microscopy (TEM), the organic ligands act as a source for e-beam induced deposition and this causes substantial build-up of carbon layers in the investigated areas, which is typically referred to as "contamination" in the field of electron microscopy. This contamination is often more severe for scanning TEM, a technique that is based on a focused electron beam and hence higher electron dose rate. In this paper, we report a simple and effective method to clean drop-cast TEM grids that contain NPs with ligands. Using a combination of activated carbon and ethanol, this method effectively reduces the amount of ligands on TEM grids, and therefore greatly improves the quality of electron microscopy images and subsequent analytical measurements. This efficient and facile method can be helpful during electron microscopy investigation of different kinds of nanomaterials that suffer from ligand-induced contamination.
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http://dx.doi.org/10.1016/j.ultramic.2020.113195DOI Listing
February 2021

A Review on Additives-assisted Ultrasound for Organic Pollutants Degradation.

J Hazard Mater 2021 02 12;403:123915. Epub 2020 Sep 12.

State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin 150090, China. Electronic address:

In the past 2 decades, considerable attentions have been paid to the sonochemical advanced oxidation processes (SAOPs) in the fields of pollutants removal. SAOPs are powerful methods for refractory pollutants degradation due to the free radicals (e.g., •OH and •H) generated by water pyrolysis and extremely high temperature and pressure in and around cavitation bubbles. Reports on various additives for the improvement of sonochemical pollutants degradation including oxidants, inorganic anions, etc. have been made. This paper presents a comprehensive review on the ultrasound (US) alone and sono-hybrid systems for various pollutants degradation. In this paper, the degradation efficiency of various pollutants in sono-hybrid systems are elucidated in detail, and particular emphasis is placed on the reaction mechanism of additives in US for the enhancement of pollutants degradation. The problems on the applications of the current sono-hybrid systems are identified and discussed, and the outlooks for further in-depth studies on the challenges and some research needs for the applications of SAOPs for the removal of organic pollutants from aquatic systems are made at the end.
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http://dx.doi.org/10.1016/j.jhazmat.2020.123915DOI Listing
February 2021

In situ chloride-mediated synthesis of TiO thin film photoanode with enhanced photoelectrochemical activity for carbamazepine oxidation coupled with simultaneous cathodic H production and CO conversion to fuels.

J Hazard Mater 2021 05 21;410:124563. Epub 2020 Nov 21.

College of Environment, Zhejiang University of Technology, Hangzhou 310032, China. Electronic address:

This study investigated the simultaneous photoelectrochemical (PEC) degradation of carbamazepine (CBZ), reduction of CO and production of H using a TiO thin film as photoanode and Ag plate as cathode. The photoanode was fabricated using sequential hydrothermal and calcination processes. The use of chloride during the hydrothermal process enhanced formation of oxygen vacancies and defects on the TiO surface. Calcination not only further strengthened those features but also enhanced the crystallinity and anatase/rutile ratio, endowing the TiO photoanode with superior PEC capacity. Characterization of physicochemical and PEC properties revealed that photogenerated electrons-holes were rapidly generated and efficiently separated on the TiO surface during the PEC process. Hydroxyl radicals were the main active species responsible for anodic oxidation of carbamazepine, while hydrogen radicals and carbon dioxide radical anions mediated CO reduction and H production in the cathodic process. This work confirms the suitability of the prepared TiO photoanode for PEC degradation of organic pollutants coupled with CO reduction and H production.
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http://dx.doi.org/10.1016/j.jhazmat.2020.124563DOI Listing
May 2021
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