Publications by authors named "Da Li"

542 Publications

Erlotinib as a salvage treatment after gefitinib failure for advanced non-small-cell lung cancer patients with brain metastasis: A successful case report and review.

Medicine (Baltimore) 2021 Jun;100(25):e26450

Department of Medical Oncology, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou.

Rationale: The guidelines recommended gefitinib as a first-line targeted treatment for stage IV non-small-cell lung cancer (NSCLC) patients with EGFR mutations. However, resistance to gefitinib ensues invariably and there is little evidence as for the effectiveness of subsequent salvage treatment for patients without T790m mutation. The case is to evaluate the efficacy of erlotinib, another EGFR-TKI, after failed first-line use of gefitinib.

Patient Concerns: We described a 55-year-old man with good performance status (PS).

Diagnoses: He was histopathologically diagnosed stage IV lung adenocarcinoma with EGFR mutations in November 2018.

Interventions: He was administrated with gefitinib daily (250 mg) for activating epidermal growth factor receptor (EGFR) mutations (exon 19 deletions,19del), and combined with platinum-based dual-drug chemotherapy. During the target treatments, the optimal efficacy evaluation was partial remission (PR) with a 12-month progression-free survival (PFS) time. Later, the intracranial progression of the patient rendered the treatment change to erlotinib.

Outcomes: It is surprising that the tumor lesion in brain as well as lung relieved obviously. His progression-free survival (PFS)was nearly 11 months, and the overall survival (OS)was>36 months up to now. The adverse events were tolerable.

Lessions: This case manifests that re-biopsy of advanced or recurrent NSCLC is beneficial to make a better therapeutic regimen, and erlotinib can be used as a salvage treatment after gefitinib failure.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/MD.0000000000026450DOI Listing
June 2021

Serum Anti-Müllerian Hormone Levels Were Negatively Associated With Body Fat Percentage in PCOS Patients.

Front Endocrinol (Lausanne) 2021 4;12:659717. Epub 2021 Jun 4.

Center of Reproductive Medicine, Shengjing Hospital of China Medical University, Shenyang, China.

Background: Obesity is a state of excess body fat accumulation, and appears to be closely associated with polycystic ovary syndrome (PCOS). Notably, plausible biological pathways through which obesity can regulate anti-Müllerian hormone (AMH) production have been proposed, and women with PCOS characteristically have an increased AMH level. Body fat accumulation can be described by body fat percentage (BFP). However, the relationship between BFP and AMH still remains unclear.

Materials And Methods: A total of 87 controls and 156 PCOS patients were divided into lean and overweight/obese groups, and the PCOS patients were further divided into hyper-AMH and normal-AMH subgroups. Univariate regression was used to assess the unadjusted relationship between AMH and outcome variables, multivariable regression analysis was performed to test whether and how serum AMH levels were associated with BFP after adjusting for other co-variables. Receiver-operating characteristic (ROC) curve analyses were used to test the utility of BFP for the diagnosis of PCOS.

Results: BFP was higher in PCOS patients compared with controls, regardless of obesity. Serum AMH levels were negatively associated with BFP in the PCOS group (r = -0.371; < 0.001) but not in the control group (r = -0.095; = 0.385). Multivariable logistic regression analysis showed that elevated BFP was associated with a high risk of PCOS (odds ratio, 1.290; 95% confidence interval, 1.084-1.534, = 0.004). Furthermore, the combination of BFP and serum AMH into a multivariate model gave an improved area under the curve (AUC) of 88.5%, with a sensitivity of 72.4% and specificity of 87.4%; the positive and negative predictive values were 91.2% and 63.9%, respectively. One limitation of this study is all the conclusion reported was based on small sample size.

Conclusions: Herein, we described the negative correlation between BFP and serum AMH levels for the first time, and the present results highlight the importance of further investigation into the role of BFP, especially in body fat-related AMH change as it relates to the underlying pathogenesis of PCOS.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fendo.2021.659717DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8213015PMC
June 2021

The characteristics of distal tears affect false lumen thrombosis rate after thoracic endovascular aortic repair for acute type B dissection.

Interact Cardiovasc Thorac Surg 2021 Jun 14. Epub 2021 Jun 14.

Beijing Advanced Innovation Center of Biomedical Engineering, Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing, China.

Objectives: A low false lumen thrombosis rate (FLTR) is common in patients with type B aortic dissection after they have undergone thoracic endovascular aortic repair, which indicates a poor long-term prognosis. This study aimed to establish a quantitative linear regression model to predict false lumen (FL) thrombosis accurately using morphological parameters.

Methods: In this retrospective study, we included 59 patients diagnosed with acute type B aortic dissection between 2014 and 2017. Morphological parameters were measured. Univariable and multivariable linear regression analyses were performed, and a linear regression model relating FLTR with the number of re-entry tears was proposed. Ten patients were further chosen to validate the linear relationship, and idealized aortic dissection models were adopted for haemodynamic analysis.

Results: Only the total area and number of re-entry tears were negatively correlated with FL thrombosis (P < 0.001). Moreover, based on the univariable regression, the number of re-entry tears played a more crucial role in FLTR (R2 = 0.509 vs R2 = 0.298), and the linear relationship model was created as follows: thrombosis rate (%) = -11.25 × distal tear number + 105.24. This model was perfectly matched in 10 patients (concordance correlation coefficient = 0.880, P = 0.947). Moreover, when the total area of re-entry tears was constant, the net blood flow increased rapidly with an increase in the tear count.

Conclusions: The number of re-entry tears could be a crucial related factor of FL thrombosis; the larger the number of re-entry tears is, the lower the possibility of thrombosis is.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/icvts/ivab166DOI Listing
June 2021

Stability of hydrogen-terminated graphene edges.

Phys Chem Chem Phys 2021 Jun;23(23):13261-13266

State Key Laboratory of Superhard Materials, College of Physics, Jilin University, Changchun, 130012, P. R. China.

Hydrogen passivation is an important method used to stabilize a specific graphene edge. Although several hydrogen-terminated graphene edges have been proposed in theory, a comprehensive exploration of highly stable hydrogen-terminated graphene edges is still absent. According to the bare graphene-edge databases, a series of hydrogen-terminated graphene edges have been proposed. The energy stability of hydrogen-terminated zigzag and armchair graphene edges is fully investigated. The six most stable hydrogen-terminated zigzag edges and six armchair edges of graphene are determined. Hydrogen passivation makes hydrogen-terminated graphene edges energetically more stable than bare graphene edges. The additional hydrogen atoms balance the dangling bonds of carbon atoms at edges by forming hydrogen-carbon covalent bonds. Hydrogen-terminated graphene edges with six-membered carbon rings have better global stability than those composed of non-hexagonal structural units. The effects of the experimental temperatures and hydrogen partial pressures on the stability of hydrogen-terminated graphene edges are fully investigated. Furthermore, hydrogen passivation can open the band gap of graphene effectively. These results provide a deep understanding of hydrogen-terminated graphene nanostructures.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d1cp01384jDOI Listing
June 2021

The thermal regime modifies the response of aquatic keystone species Daphnia to microplastics: Evidence from population fitness, accumulation, histopathological analysis and candidate gene expression.

Sci Total Environ 2021 Aug 17;783:147154. Epub 2021 Apr 17.

Jiangsu Key Laboratory for Biodiversity and Biotechnology, School of Biological Sciences, Nanjing Normal University, Nanjing 210023, China. Electronic address:

The water bodies are greatly influenced by chemical contamination and global increasing temperature. As an emerging pollutant, microplastics are widely distributed in the freshwater environment, raising concerns regarding their potential toxicity to organisms. Especially for zooplankton filter feeders, many of microplastics are in similar size as their food. Individually, both microplastics and temperature have profound effects on zooplankton populations and their function in ecosystems. However, the strength and direction of their interactive effects are still not clear. Here, we performed a comprehensive biotoxicity assessment providing empirical evidence that the temperature played a key role in shaping the sensitivity of the zooplankter, Daphnia magna, against microplastic toxicity. We found that exposure to microplastics generally caused negative effects on Daphnia individual fitness, such as increased lethality, declined fecundity and reduced population growth rate. This microplastic toxicity was more prominent at 30 °C than at 20 °C, and was rather minor at 15 °C. Moreover, the warming accelerated the ingestion of microplastics, and triggered abnormal ultrastructure of intestinal epithelial cells. In addition, the expression profiling of candidate genes revealed oxidative damage, fecundity impairment and energy retardation by microplastics were amplified with increasing temperature, which may contribute to the enhancement of microplastic toxicity under warming. Given that high temperature fluctuations are becoming more common and difficult to predict, the interactive effects of microplastics and climate warming on Daphnia population dynamics and biomass production may become increasingly aggravated in nature. Collectively, extrapolation for environmental risk assessment studies conducted under different temperature contexts may broaden our knowledge microplastic toxicity on aquatic organism fitness.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2021.147154DOI Listing
August 2021

Expansion and hazard risk assessment of glacial lake Jialong Co in the central Himalayas by using an unmanned surface vessel and remote sensing.

Sci Total Environ 2021 Aug 21;784:147249. Epub 2021 Apr 21.

State Key Laboratory of Cryospheric Science, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou 730000, China; University of Chinese Academy of Sciences, Beijing 100049, China.

Glacial lake outburst floods (GLOFs) are a severe hazard in the Himalayas. Glacial lake expansion and the corresponding volume increase play major roles in GLOFs as well as climate change. Furthermore, mass movement and dam conditions play a major role in the GLOF initiation process. Recently, because of global warming, glacial lakes in the central Himalayas have been expanding rapidly. Owing to a lack of systematic assessment and meticulous field surveys, people living downstream are at great risk of GLOFs. Comprehensive investigations and assessment of the relationships among lake expansion, lake dam conditions, and GLOF risk are urgently needed. In this study, we surveyed Jialong Co, a typical end-moraine dammed lake in Poiqu River in the central Himalayas by using Landsat and Sentinel satellite images from the past 32 years, field work, and depth measurements using an unmanned surface vessel on August 28, 2020. The results showed that Jialong Co had experienced slow-quick-slow expansion, increasing in area from 0.13 ± 0.03 to 0.60 ± 0.02 km. The lake bathymetric map revealed that the lake volume was (3.75 ± 0.38) × 10 m in 2020. Lake expansion occurred in the area from which the mother glacier retreated, indicating a close connection between the lake and its mother glacier and revealing that topography controlled the lake expansion process. Furthermore, thorough field work revealed that outlet dynamics and external water erosion are vulnerable elements in the disaster chain that initiate and affect the GLOF hazard of Jialong Co. Overall, this case study could help scholars understand the expansion mechanism of end-moraine dammed lakes and aid in hazard assessment of glacial lakes in the central Himalayas.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2021.147249DOI Listing
August 2021

Effects of polyurethane foam carrier addition on anoxic/aerobic membrane bioreactor (A/O-MBR) for coal gasification wastewater (CGW) treatment: Performance and microbial community structure.

Sci Total Environ 2021 May 26;789:148037. Epub 2021 May 26.

State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin 150090, China. Electronic address:

Coal gasification wastewater (CGW) is a typical toxic and refractory industrial wastewater with abundant phenols contained. Two identical anoxic/aerobic membrane bioreactors (with (R2) and without (R1) polyurethane (PU) foam) were carried out in parallel to investigate the role of PU foam addition in enhancing pollutants removal in CGW. Results showed that both systems exhibited effective removal of chemical oxygen demand (>93%) and total phenols (>97%) but poor ammonia nitrogen removal (<35%) constrained by ammonia oxidation process. GC-MS analysis revealed that aromatic and other refractory intermediates were dramatically reduced in R2. Moreover, the PU addition had negligible influence on the total soluble microbial products and extracellular polymeric substances contents but significantly alleviated membrane fouling with the operating time 33% prolonged. Microbial community revealed that Flavobacterium, Holophaga, and Geobacter were enriched on PU. Influent type might be a main driver for microbial community succession.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2021.148037DOI Listing
May 2021

16S rDNA Full-Length Assembly Sequencing Technology Analysis of Intestinal Microbiome in Polycystic Ovary Syndrome.

Front Cell Infect Microbiol 2021 10;11:634981. Epub 2021 May 10.

Center of Reproductive Medicine, Shengjing Hospital of China Medical University, Shenyang, China.

Objective: To study the characteristics and relationship of the gut microbiota in patients with polycystic ovary syndrome (PCOS).

Method: We recruited 45 patients with PCOS and 37 healthy women from the Reproductive Department of Shengjing Hospital. We recorded their clinical indexes, and sequenced their fecal samples by 16S rDNA full-length assembly sequencing technology (16S-FAST).

Result: We found decreased α diversity and different abundances of a series of microbial species in patients with PCOS compared to healthy controls. We found LH and AMH were significantly increased in PCOS with enterotype when compared to control women with enterotype, while glucose and lipid metabolism level remained no significant difference, and situations were opposite in PCOS and control women with enterotype. and were more abundant while spp. were less abundant in the PCOS group. was significantly more prevalent in PCOS-not insulin resistance (NIR) compared to control-NIR and PCOS-not overweight (NOW) patient groups compared to control-NOW groups. Kyoto Encyclopedia Genes and Genomes reflecting pathways related to lipopolysaccharide biosynthesis were more abundant in the PCOS group.

Conclusion: Our study found gut microbiota that had different abundance in patients with PCOS compared to healthy controls. An intimate relationship was shown between the gut microbiota and pathological changes in PCOS. We suggest the gut microbiota should be taken into consideration in the treatment of symptoms of PCOS drugs and diet.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fcimb.2021.634981DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8141595PMC
May 2021

Novel biallelic mutations in MEI1: expanding the phenotypic spectrum to human embryonic arrest and recurrent implantation failure.

Hum Reprod 2021 May 25. Epub 2021 May 25.

Institute of Pediatrics, Children's Hospital of Fudan University and Institutes of Biomedical Sciences, State Key Laboratory of Genetic Engineering, Fudan University, Shanghai, China.

Study Question: Are any novel mutations and corresponding new phenotypes, other than recurrent hydatidiform moles, seen in patients with MEI1 mutations?

Summary Answer: We identified several novel mutations in MEI1 causing new phenotypes of early embryonic arrest and recurrent implantation failure.

What Is Known Already: It has been reported that biallelic mutations in MEI1, encoding meiotic double-stranded break formation protein 1, cause azoospermia in men and recurrent hydatidiform moles in women.

Study Design, Size, Duration: We first focused on a pedigree in which two sisters were diagnosed with recurrent hydatidiform moles in December 2018. After genetic analysis, two novel mutations in MEI1 were identified. We then expanded the mutational screening to patients with the phenotype of embryonic arrest, recurrent implantation failure, and recurrent pregnancy loss, and found another three novel MEI1 mutations in seven new patients from six families recruited from December 2018 to May 2020.

Participants/materials, Setting, Methods: Nine primary infertility patients were recruited from the reproduction centers in local hospitals. Genomic DNA from the affected individuals, their family members, and healthy controls was extracted from peripheral blood. The MEI1 mutations were screened using whole-exome sequencing and were confirmed by the Sanger sequencing. In silico analysis of mutations was performed with Sorting Intolerant From Tolerant (SIFT) and Protein Variation Effect Analyzer (PROVEAN). The influence of the MEI1 mutations was determined by western blotting and minigene analysis in vitro.

Main Results And The Role Of Chance: In this study, we identified five novel mutations in MEI1 in nine patients from seven independent families. Apart from recurrent hydatidiform moles, biallelic mutations in MEI1 were also associated with early embryonic arrest and recurrent implantation failure. In addition, we demonstrated that protein-truncating and missense mutations reduced the protein level of MEI1, while the splicing mutations caused abnormal alternative splicing of MEI1.

Limitations, Reasons For Caution: Owing to the lack of in vivo data from the oocytes of the patients, the exact molecular mechanism(s) involved in the phenotypes remains unknown and should be further investigated using knock-out or knock-in mice.

Wider Implications Of The Findings: Our results not only reveal the important role of MEI1 in human oocyte meiosis and early embryonic development, but also extend the phenotypic and mutational spectrum of MEI1 and provide new diagnostic markers for genetic counseling of clinical patients.

Study Funding/competing Interest(s): This work was supported by the National Key Research and Development Program of China (2018YFC1003800, 2017YFC1001500, and 2016YFC1000600), the National Natural Science Foundation of China (81725006, 81822019, 81771581, 81971450, and 81971382), the project supported by the Shanghai Municipal Science and Technology Major Project (2017SHZDZX01), the Project of the Shanghai Municipal Science and Technology Commission (19JC1411001), the Natural Science Foundation of Shanghai (19ZR1444500), the Shuguang Program of the Shanghai Education Development Foundation and the Shanghai Municipal Education Commission (18SG03), the Shanghai Health and Family Planning Commission Foundation (20154Y0162), the Strategic Collaborative Research Program of the Ferring Institute of Reproductive Medicine, Ferring Pharmaceuticals and the Chinese Academy of Sciences (FIRMC200507) and the Chongqing Key Laboratory of Human Embryo Engineering (2020KFKT008). No competing interests are declared.

Trial Registration Number: N/A.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/humrep/deab118DOI Listing
May 2021

Treatment and prognostic factors of pituicytoma: a single-center experience and comprehensive literature review.

Pituitary 2021 May 12. Epub 2021 May 12.

Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, No.119 South Fourth Ring West Road, Fengtai District, Beijing, 100070, People's Republic of China.

Purpose: Preoperative diagnosis of pituicytomas is difficult, and management and prognostic factors remain ambiguous. The purpose of this study was to elucidate the radiological characteristics of pituicytoma, to assess the risk factors affecting tumor progression, and to propose the optimal treatment regimen based on comprehensive analysis.

Methods: We reviewed the clinical data of 22 patients with pituicytoma confirmed pathologically in our institution. In addition, 93 cases of pituicytoma in the previous literature were recruited. The individual data of 115 patients were analyzed to evaluate the adverse factors affecting pituicytoma progression.

Results: In the combined cohort, 3 of 61 patients who underwent gross-total resection (GTR) developed recurrence (4.9%); of the 54 patients who received non-GTR, 19 progressed (35.2%). Univariate and multivariate Cox regression analysis verified male gender (HR 2.855, 95% CI 1.008-8.089; p = 0.048), TS (transsphenoidal surgery; HR 3.559, 95% CI 1.015-12.476; p = 0.047), and non-GTR (HR 4.388, 95%CI 1.240-15.521; p = 0.022) were independent unfavorable factors for pituicytoma progression. A multivariate logistic regression model verified that tumor diameter ≥ 1.85 cm (OR 4.859, 95% CI 1.335-17.691; p = 0.016) was independent adverse factors for GTR. Compared with TS, OT (open transcranial) is more likely to have postoperative complications (OR 3.185, 95% CI 1.020-9.944; p = 0.046), especially vision deterioration (OR 37.267, 95% CI 4.486-309.595; p = 0.001).

Conclusion: Based on our findings, GTR was advocated as an optimal treatment for pituicytomas. However, in order to avoid damage to important structures, partial resection is acceptable. After that, adjuvant radiotherapy is recommended for male patients with high Ki-67 index, and the remaining patients can be followed up closely. When the tumor recurs or progresses, it is recommended to re-operate and remove the lesion completely as far as possible. If GTR is still not possible, postoperative radiotherapy for the residual tumor is recommended.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11102-021-01152-5DOI Listing
May 2021

Anisotropic Angstrom-Wide Conductive Channels in Black Phosphorus by Top-down Cu Intercalation.

Nano Lett 2021 May 5. Epub 2021 May 5.

Center for Multidimensional Carbon Materials, Institute for Basic Science (IBS), Ulsan 44919, Republic of Korea.

Intercalation in black phosphorus (BP) can induce and modulate a variety of the properties including superconductivity like other two-dimensional (2D) materials. In this perspective, spatially controlled intercalation has the possibility to incorporate different properties into a single crystal of BP. We demonstrate anisotropic angstrom-wide (∼4.3 Å) Cu intercalation in BP, where Cu atoms are intercalated along a zigzag direction of BP because of its inherent anisotropy. With atomic structure, its microstructural effects, arising from the angstrom-wide Cu intercalation, were investigated and extended to relation with macrostructure. As the intercalation mechanism, it was revealed by in situ transmission electron microscopy and theoretical calculation that Cu atoms are intercalated through top-down direction of BP. The Cu intercalation anisotropically induces transition of angstrom-wide electronic channels from semiconductor to semimetal in BP. Our findings throw light on the fundamental relationship between microstructure changes and properties in intercalated BP, and tailoring anisotropic 2D materials at angstrom scale.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.nanolett.1c00915DOI Listing
May 2021

N6-methyladenosine demethylase FTO impairs hepatic ischemia-reperfusion injury via inhibiting Drp1-mediated mitochondrial fragmentation.

Cell Death Dis 2021 May 4;12(5):442. Epub 2021 May 4.

Department of Hepatic Surgery, Zhongshan Hospital, Fudan University, Shanghai, China.

Despite N6-methyladenosine (m6A) is functionally important in various biological processes, its role and the underlying regulatory mechanism in the liver remain largely unexplored. In the present study, we showed that fat mass and obesity-associated protein (FTO, an m6A demethylase) was involved in mitochondrial function during hepatic ischemia-reperfusion injury (HIRI). We found that the expression of m6A demethylase FTO was decreased during HIRI. In contrast, the level of m6A methylated RNA was enhanced. Adeno-associated virus-mediated liver-specific overexpression of FTO (AAV8-TBG-FTO) ameliorated the HIRI, repressed the elevated level of m6A methylated RNA, and alleviated liver oxidative stress and mitochondrial fragmentation in vivo and in vitro. Moreover, dynamin-related protein 1 (Drp1) was a downstream target of FTO in the progression of HIRI. FTO contributed to the hepatic protective effect via demethylating the mRNA of Drp1 and impairing the Drp1-mediated mitochondrial fragmentation. Collectively, our findings demonstrated the functional importance of FTO-dependent hepatic m6A methylation during HIRI and provided valuable insights into the therapeutic mechanisms of FTO.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41419-021-03622-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8096847PMC
May 2021

Case Report: Clinical Responses to Tislelizumab as a First-Line Therapy for Primary Hepatocellular Carcinoma With B-Cell Indolent Lymphoma.

Front Immunol 2021 31;12:634559. Epub 2021 Mar 31.

Department of Medical Oncology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Background: As an emerging therapy with a promising efficacy, immunotherapy has been widely used in the treatment of solid tumors and hematologic malignancies. This clinical study compares the efficacy of tislelizumab, a domestic immune checkpoint inhibitor (ICI), to that of sorafenib when used as a first-line therapeutic option in hepatocellular carcinoma (HCC), and the concurrence of HCC and non-Hodgkin's lymphoma (NHL) is rare, especially in the treatment of ICIs.

Case Presentation: A 61-year-old patient presenting with primary HCC and indolent B-cell lymphoma had a partial clinical response to tislelizumab for his primary HCC. Besides, we described a phenomenon of pseudo-progression and delayed diagnosis of his lymphoma during a long course of treatment.

Conclusion: Tislelizumab, an immunotherapeutic option with a favorable efficacy and toxicity, can be used to manage double primary tumors. However, studies should aim to elucidate the probable mechanisms of this therapy. Pseudo-progression and separation remission make the treatment of double primary tumors even more challenging, which calls for additional caution in patients undergoing immunotherapy to avoid misdiagnosis and, therefore, begin early appropriate interventions.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fimmu.2021.634559DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8044442PMC
March 2021

An Insight of the First Community Infected COVID-19 Patient in Beijing by Imported Case: Role of Deep Learning-Assisted CT Diagnosis.

Chin Med Sci J 2021 Mar;36(1):66-71

Department of Infection, Beijing Haidian Section of Peking University Third Hospital (Beijing Haidian Hospital), Beijing 100080, China.

In the era of coronavirus disease 2019 (COVID-19) pandemic, imported COVID-19 cases pose great challenges to many countries. Chest CT examination is considered to be complementary to nucleic acid test for COVID-19 detection and diagnosis. We report the first community infected COVID-19 patient by an imported case in Beijing, which manifested as nodular lesions on chest CT imaging at the early stage. Deep Learning (DL)-based diagnostic systems quantitatively monitored the progress of pulmonary lesions in 6 days and timely made alert for suspected pneumonia, so that prompt medical isolation was taken. The patient was confirmed as COVID-19 case after nucleic acid test, for which the community transmission was prevented timely. The roles of DL-assisted diagnosis in helping radiologists screening suspected COVID cases were discussed.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.24920/003788DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8041135PMC
March 2021

Carbon and Oxygen Coordinating Atoms Adjust Transition Metal Single-Atom Catalysts Based On Boron Nitride Monolayers for Highly Efficient CO Electroreduction.

ACS Appl Mater Interfaces 2021 Apr 14;13(16):18934-18943. Epub 2021 Apr 14.

School of Physical Science and Technology, Ningbo University, Ningbo 315211, P.R. China.

Although single-atom catalysts (SACs) with transition metal-nitrogen complexes have been studied widely, investigations that use light-element atoms to adjust the coordination environment of the central metal atoms in metal-nitrogen complexes are still rare but show enormous potential for various electrocatalytic reactions. Herein, we design novel SACs based on monolayer BN adjusted by B, C, or O coordinating atoms as catalysts for the CO reduction reaction (CRR). These SACs are denoted as [email protected]_D (BN = monolayer boron nitride; D = B, C, or O atom; M = Co, Cr, Fe, Mn, Mo, Pd, Pt, Ru, V, W, Ni, Zn, Zr, Ag, Au, Cu, or Ti atom) and are investigated as CRR catalysts using density functional theory calculations. Among these structures, we identified some promising candidate catalysts for CRR with impressive low limiting potential (): [email protected]_C with a of -0.18 for the product CH and [email protected]_C and [email protected]_O with of -0.41 and -0.37 V, respectively, for the product CHOH. In particular, [email protected]_C shows a remarkable reduction in for the product CH compared to any existing catalysts, synthesized or predicted. In addition, the ultralow for CRR on [email protected]_C was derived from the unique bonding feature between the single metal atom and adsorbates and the modulation of ionic interactions induced by the coordination effect of the C atom.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.1c04580DOI Listing
April 2021

Anisotropic Growth and Magnetic Properties of α″[email protected] Nanocones.

Nanomaterials (Basel) 2021 Mar 31;11(4). Epub 2021 Mar 31.

Shenyang National Laboratory for Materials Science, Institute of Metal Research, Chinese Academy of Sciences, 72 Wenhua Road, Shenyang 110016, China.

α″-FeN nanomaterials with a shape anisotropy for high coercivity performance are of interest in potential applications such as rare-earth-free permanent magnets, which are difficult to synthesize in situ anisotropic growth. Here, we develop a new and facile one-pot microemulsion method with Fe(CO) as the iron source and tetraethylenepentamine (TEPA) as the N/C source at low synthesis temperatures to fabricate carbon-coated tetragonal α″-FeN nanocones. Magnetocrystalline anisotropy energy is suggested as the driving force for the anisotropic growth of α″[email protected] nanocones because the easy magnetization direction of tetragonal α″-FeN nanocrystals is along the c axis. The α″[email protected] nanocones agglomerate to form a fan-like microstructure, in which the thin ends of nanocones direct to its center, due to the magnetostatic energy. The lengths of α″[email protected] nanocones are ~200 nm and the diameters vary from ~10 nm on one end to ~40 nm on the other end. Carbon shells with a thickness of 2-3 nm protect α″-FeN nanocones from oxidation in air atmosphere. The α″[email protected] nanocones synthesized at 433 K show a room-temperature saturation magnetization of 82.6 emu/g and a coercive force of 320 Oe.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/nano11040890DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8065777PMC
March 2021

Remediation of saline-sodic soil by plant microbial desalination cell.

Chemosphere 2021 Aug 16;277:130275. Epub 2021 Mar 16.

School of Environment, State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, No 73 Huanghe Road, Nangang District, Harbin, 150090, China. Electronic address:

Saline-sodic soil is widely distributed around the world and has induced severe impacts on ecosystems and agriculture. Plant microbial desalination cell (PMDC) and soil microbial desalination cell (SMDC) were constructed to migrate excessive salt in the soil in this study. Compared with SMDC, PMDCs generated higher voltage ranging from 150 mV to 410 mV (500Ω) and the maximum power density reached 34 mW/m. Higher desalinization efficiency was obtained by PMDCs, the soil conductivity reduced from initial 2.4 mS/cm to 0.4 ± 0.1 mS/cm and pH decreased from initial 10.4 to 8.2 ± 0.1. Soils desalination in PMDCs was achieved through multiple pathways, including ion migration in PMDCs driven by electrokinetic process, plant absorption and bioremediation by plant roots and anode microorganism activity. Geobacter was the dominant electrogenic bacteria at the PMDC anode. The electrochemical and desalinating performance of PMDCs was enhanced by plants and provided a new method for remediation of saline-sodic soil.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.chemosphere.2021.130275DOI Listing
August 2021

Influence of Distal Re-entry Tears on False Lumen Thrombosis After Thoracic Endovascular Aortic Repair in Type B Aortic Dissection Patients: A Computational Fluid Dynamics Simulation.

Cardiovasc Eng Technol 2021 Mar 25. Epub 2021 Mar 25.

Beijing Advanced Innovation Center of Biomedical Engineering, Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing, 100191, China.

Purpose: Distal re-entry tears play a significant role in false lumen (FL) thrombosis, which will strongly affect the postoperative long-term survival of patients with type B aortic dissection (TBAD) after thoracic endovascular aortic repair (TEVAR). This study aimed to investigate the influence of a peculiar morphological parameter of the residual re-entry tears in TBAD patients after TEVAR on long-term FL thrombosis using the computational fluid dynamics.

Methods: Ideal population-based three-dimensional models of post-operative TBAD were established. Numerical simulation was performed to investigate the hemodynamic differences caused by different tear features, including the tear count, the maximum distance between tears, and the tear area.

Results: Although the low relative residence time (RRT) area did not change significantly when the tear distance was fixed, the area of oscillatory shear index (OSI) > 0.45 and endothelial cell activation potential (ECAP) > 1.5 decreased significantly with the tear count and area increased and a dramatic increase in blood flow into the FL was also observed. When tear count and total area were fixed, for each 10-mm increase in the maximum distance between tears, the area of low RRT in the FL increased significantly, while the average pressure difference increased by 10.85%.

Conclusion: The different morphology of the re-entry tears had different effects on the thrombosis-related hemodynamic parameters in FL following TEVAR. and the number of re-entry tears was most crucial to the potential thrombosis in the post-TEVAR FL of TBAD patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s13239-021-00532-zDOI Listing
March 2021

Prenatal Hypoxia Altered Angiotensin II-mediated Vasoconstrictions PKC/ERK/ROCK Pathways and Potassium Channels in Rat Offsrping Middle Cerebral Artery.

Biomed Environ Sci 2021 Mar;34(3):250-255

Reproductive Medicine Center, The First Affiliated Hospital of Soochow University, Suzhou 215000, Jiangsu, China.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3967/bes2021.033DOI Listing
March 2021

Novel Nutrition-Based Nomograms to Assess the Outcomes of Lung Cancer Patients Treated With Anlotinib or Apatinib.

Front Oncol 2021 8;11:628693. Epub 2021 Mar 8.

Department of Medical Oncology, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou, China.

Background: Previous studies have indicated that the changes in body composition during treatment are prognostic in lung cancer. The question which follows is it may be too late to identify vulnerable patients after treatment and to improve outcomes for these patients. In our study, we sought to explore the alterations of body composition and weight before the outset of the antiangiogenic treatment and its role in predicting clinical response and outcomes.

Methods: In this retrospective study, 122 patients with advanced lung cancer treated with anlotinib or apatinib were analyzed. The changes in weight and body composition including skeletal muscle index (SMI), subcutaneous adipose tissue (SAT), and visceral adipose tissue (VAT) for 3 months before the outset of antiangiogenic treatment and other clinical characteristics were evaluated with LASSO Cox regression and multivariate Cox regression analysis, which were applied to construct nomograms. The performance of the nomograms was validated internally by using bootstrap method with 1,000 resamples models and was assessed by the concordance index (C-index), calibration plots, decision curve analysis (DCA).

Results: The median progression-free survival (PFS) and overall survival (OS) were 128 (95% CI 103.2-152.8) days and 292 (95% CI 270.9-313.1) days. Eastern Cooperative Oncology Group performance status (ECOG PS), brain metastases, the Glasgow Prognostic Score (GPS), clinical response, therapeutic regimen, and ΔL1SMI per 90 days were significantly associated with PFS, while ECOG PS, GPS, clinical response, therapeutic regimen, ΔL1SMI per 90 days were identified for OS. The C-index for the nomograms of PFS and OS were 0.763 and 0.748, respectively. The calibration curves indicated excellent agreement between the predicted and actual survival outcomes of 3- and 4-month PFS and 7- and 8-month OS. DCA showed the considerable value of the model.

Conclusion: Nomograms were developed from clinical features and nutritional indicators to predict the probability of achieving 3-month and 4-month PFS and 7-month and 8-month OS with antiangiogenic therapy for advanced lung cancer. Dynamic changes in body composition before the initiation of treatment contributed to early detection of poor outcome.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fonc.2021.628693DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7982902PMC
March 2021

Highly elevated levels, infant dietary exposure and health risks of medium-chain chlorinated paraffins in breast milk from China: Comparison with short-chain chlorinated paraffins.

Environ Pollut 2021 Jun 10;279:116922. Epub 2021 Mar 10.

State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China.

Short-chain chlorinated paraffins (SCCPs) are persistent organic pollutants which are toxic to human. Median-chain chlorinated paraffins (MCCPs) have similar toxicity to SCCPs. The productions of chlorinated paraffins (CPs) in China were 1 million tons in 2013 and remained high after that, which may lead to high risks for human exposure to CPs. To investigate temporal trends and health risks of SCCPs and MCCPs in breast milk in China, samples (n = 2020) were collected from urban and rural areas of 11 Chinese provinces in 2017 and mixed into 42 pooled samples. SCCPs and MCCPs were analyzed by two-dimensional gas chromatography with electron-capture negative-ionization mass spectrometry (GC × GC-ECNI-MS). The MCCP concentrations (median (range)) were 472 (94-1714) and 567 (211-1089) ng g lipid in urban and rural areas, respectively, which showed continuously rapidly increasing during 2007-2017. The SCCP concentrations (median (range)) were 393 (131-808) and 525 (139-1543) ng g lipid in urban and rural areas, respectively. The results showed SCCP levels decreased in urban areas between 2007 and 2017. Significant increases in MCCP/SCCP ratios might arise from extensive manufacturing and use of MCCPs. The median estimated dietary intake via breast milk in urban and rural samples were 1230 and 2510 ng kg d, respectively, for SCCPs and 2150 and 1890 ng kg d, respectively, for MCCPs. Preliminarily risk assessment showed that SCCPs posed a significant health risk to infants via breastfeeding. The high MCCP levels should also be of concern because of continuous growth and negative effect on infants. Correspondence analysis indicated congeners with higher carbon and chlorine numbers in dietary tend to accumulate in breast milk.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.envpol.2021.116922DOI Listing
June 2021

Neurological outcomes of untreated brainstem cavernous malformations in a prospective observational cohort and literature review.

Stroke Vasc Neurol 2021 Mar 18. Epub 2021 Mar 18.

Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China

Background: Haemorrhages of brainstem cavernous malformations (CMs) can lead to neurological deficits, the natural history of which is uncertain. The study aimed to evaluate the neurological outcomes of untreated brainstem CMs and to identify the adverse factors associated with worsened outcomes.

Methods: From 2009 to 2015, 698 patients (321 women) with brainstem CMs were entered into the prospective cohort after excluding patients lost to follow-up (n=43). All patients were registered, clinical data were collected and scheduled follow-up was performed.

Results: After a median follow-up of 60.9 months, prospective haemorrhages occurred in 167 patients (23.9%). The mean modified Rankin Scale scores at enrolment and at censoring time were 1.6 and 1.2. Neurological status was improved, unchanged and worsened in 334 (47.9%), 293 (42.0%) and 71 (10.2%) patients, respectively; 233 (33.4%) recovered to normal levels. Lesions crossing the axial midpoint (relative risk (RR) 2.325, p=0.003) and developmental venous anomaly (DVA) (RR 1.776, p=0.036) were independently significantly related to worsened outcomes. The percentage of worsened outcomes was 5.3% (18 of 337) in low-risk patients (neither DVA nor crossing the axial point) and increased to 26.0% (13 of 50) in high-risk patients (with both DVA and crossing the axial point). The percentage of worsened outcomes significantly increased as the number of prospective haemorrhages increased (from 1.5% (8 of 531, if 0 prospective ictus) to 37.5% (48 of 128, if 1 ictus) and 38.5% (15 of 39, if >1 ictus)).

Conclusions: The neurological outcomes of untreated brainstem CMs were improved/unchanged in majority of patients (89.8%) with a fatality rate of 1.7% in our cohort, which seemed to be favourable. Radiological features significantly predicted worsened outcomes. Our results provide evidence for clinical consultation and individualised treatment. The referral bias of our cohort was underlined.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1136/svn-2020-000608DOI Listing
March 2021

The function of BAP18 on modulation of androgen receptor action in luteinized granulosa cells from normal weight women with and without PCOS.

Mol Cell Endocrinol 2021 05 1;527:111228. Epub 2021 Mar 1.

Center of Reproductive Medicine, Shengjing Hospital of China Medical University, Shenyang City, Liaoning Province, 110004, China. Electronic address:

Polycystic ovary syndrome (PCOS) is one of the most common endocrine disorders in reproductive-age women. In this study, BPTF associated protein of 18 kDa (BAP18) is decreased in luteinized granulosa cells (GCs) from PCOS women. BAP18 depletion significantly decreases CYP19A1 expression levels, leading to an abrogation in transfer capacity of androgen to estrogen in GCs. Also, BAP18 knockdown delays cell cycle G1 to S phase transition and induces cell apoptosis to decrease GCs proliferation. We also provide evidence showing BAP18 interacts with androgen receptor (AR) and enhances AR-mediated transactivation in GCs. Results indicate that AR or BAP18 recruits to androgen response elements (AREs) of CYP19A1 and FSHR, which are putative AR-induced genes in GCs. BAP18 interacts with Sp1 transcription factor and co-recruits to the promoter region of AR gene, resulting in AR transactivation in GCs. Taken together, these data provide new insights on the pathophysiology of PCOS.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.mce.2021.111228DOI Listing
May 2021

Psychological and occupational impact on healthcare workers and its associated factors during the COVID-19 outbreak in China.

Int Arch Occup Environ Health 2021 Mar 3. Epub 2021 Mar 3.

Department of Neurology, HwaMei Hospital, University of Chinese Academy of Sciences, Xibei Rd#41, Ningbo, 315010, Zhejiang, China.

Purpose: China was affected by an outbreak of coronavirus disease 2019 (COVID-19) in 2019-2020. Research data are needed to develop evidence-driven strategies to reduce the adverse psychological and occupational impacts on healthcare workers (HCWs).

Methods: From March 1, 2020, to March 8, 2020, 946 HCWs in China completed a survey consist of sociodemographic data, precautionary measures against COVID-19, and concerns about COVID-19. Self-administered questionnaire were collected to assess psychological and occupational adverse outcomes of HCWs. Multivariable logistic regression analysis was performed to identify factors associated with the outcomes.

Results: A total of 55.0%, 56.0% and 48.3% of the HCWs experienced burnout, psychological distress and posttraumatic stress, respectively. A total of seven factors were independently associated with burnout: good health status (OR 0.51, 95% CI 0.36-0.71), fear of contagion (OR 1.31, 95% CI 1.003-1.79), avoiding contact with children (OR 1.40, 95% CI 1.03-1.91), enough staff support at the workplace (OR 0.59, 95% CI 0.38-0.92), having to work overtime (OR 1.37, 95% CI 1.03-1.83), maladaptive coping (OR 3.28, 95% CI 2.42-4.45) and adaptive coping (OR 0.47, 95% CI 0.35-0.62). A total of 11 factors were independently associated with high psychological distress: having one child (OR 0.54, 95% CI 0.38-0.77), good health status (OR 0.57, 95% CI 0.39-0.83), alcohol abuse (OR 1.51, 95% CI 1.02-2.25), thinking the epidemic would continue for quite a long time (OR 1.59, 95% CI 1.08-2.34), wearing extra-work clothes (OR 1.51, 95% CI 1.06-2.15),effective protective equipment (OR 0.45, 95% CI 0.22-0.90), enough staff support at the workplace (OR 0.55, 95% CI 0.34-0.89), unable to take care of families (OR 1.99, 95% CI 1.42-2.78), economic losses (OR 1.62, 95% CI 1.14-2.31), maladaptive coping (OR 6.88, 95% CI 4.75-9.97),and adaptive coping (OR 0.29, 95% CI 0.21-0.41). These factors were independently associated with posttraumatic stress: living with the elderly (OR 1.46, 95% CI 1.04-2.05), alcohol abuse (OR 1.41, 95% CI 1.002-1.98), working at a 3A hospital(OR 0.66, 95% CI 0.49-0.88), acquaintances confirmed COVID-19 (OR 2.14, 95% CI 1.20-3.84), fear of contagion (OR 1.87, 95% CI 1.40-2.50), believing they would survive if infected (OR 0.63, 95% CI 0.46-0.86), self-disinfected after arriving home (OR 1.43, 95% CI 1.01-2.02), interpersonal isolation (OR 1.65, 95% CI 1.21-2.26), unable to take care of families (OR 1.41, 95% CI 1.05-1.88) and maladaptive coping (OR 3.09, 95% CI 2.32-4.11).

Conclusion: The variance in adverse outcomes was explained by the effect of various factors, which will help policymakers better prepare for subsequent potential outbreaks of COVID-19.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00420-021-01657-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7926194PMC
March 2021

RNA m6A methylation orchestrates cancer growth and metastasis via macrophage reprogramming.

Nat Commun 2021 03 2;12(1):1394. Epub 2021 Mar 2.

The State Key Laboratory of Cancer Biology, Department of Immunology, Fourth Military Medical University, Xi'an, Shaanxi, China.

N6-methyladenosine (m6A) is a reversible mRNA modification that has been shown to play important roles in various biological processes. However, the roles of m6A modification in macrophages are still unknown. Here, we discover that ablation of Mettl3 in myeloid cells promotes tumour growth and metastasis in vivo. In contrast to wild-type mice, Mettl3-deficient mice show increased M1/M2-like tumour-associated macrophage and regulatory T cell infiltration into tumours. m6A sequencing reveals that loss of METTL3 impairs the YTHDF1-mediated translation of SPRED2, which enhances the activation of NF-kB and STAT3 through the ERK pathway, leading to increased tumour growth and metastasis. Furthermore, the therapeutic efficacy of PD-1 checkpoint blockade is attenuated in Mettl3-deficient mice, identifying METTL3 as a potential therapeutic target for tumour immunotherapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41467-021-21514-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7925544PMC
March 2021

Multivariant ligands stabilize anionic solvent-oriented α-CsPbX nanocrystals at room temperature.

Nanoscale 2021 Mar 24;13(9):4899-4910. Epub 2021 Feb 24.

State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, People's Republic of China.

Cubic phase CsPbX nanocrystals (NCs) are promising candidates for optoelectronic applications. However, their chemical stability heavily depends on the dynamic ionic surface. In this work, based on the interdependency of the ligands and the reaction solvent, a protocol is developed for high-quality α-CsPbX under ambient conditions. Utilizing this method, the size and full width at half maximum of CsPbX NCs can be simply tuned via changing the cationic ligands or reaction solvent, such as CHCl, CHCl, or toluene. One remarkable result is the synthesis of cubic CsPbI NCs, for which large-scale syntheses have not been reported in the literature except for our method, due to significant phase transition at room temperature. Another result is that we have realized ultrasmall sized CsPbCl NCs with emission at 385 nm for the first time. Furthermore, the elimination of reaction solvent (such as ODE, DMSO, DMF) in our protocol reduces the purification-induced surface ligand loss and the irreversible phase transition to a nonfluorescent phase. Our CsPbX NCs show near-perfect photoluminescence quantum yield (PL QY) and long-term stability in the presence of moisture. Further characterization demonstrates that all the ligands, whether the initial paired X type or the degenerated hybrid L-X type, remain perfectly passivating on the defect sites throughout.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d0nr08697eDOI Listing
March 2021

Exceptionally preserved early Cambrian bilaterian developmental stages from Mongolia.

Nat Commun 2021 02 15;12(1):1037. Epub 2021 Feb 15.

School of Earth Sciences, University of Bristol, Bristol, UK.

Fossilized invertebrate embryonic and later developmental stages are rare and restricted largely to the Ediacaran-Cambrian, providing direct insight into development during the emergence of animal bodyplans. Here we report a new assemblage of eggs, embryos and bilaterian post-embryonic developmental stages from the early Cambrian Salanygol Formation of Dzhabkan Microcontinent of Mongolia. The post-embryonic developmental stages of the bilaterian are preserved with cellular fidelity, possessing a series of bilaterally arranged ridges that compare to co-occurring camenellan sclerites in which the initial growth stages retain the cellular morphology of modified juveniles. In this work we identify these fossils as early post-embryonic developmental stages of camenellans, an early clade of stem-brachiopods, known previously only from isolated sclerites. This interpretation corroborates previous reconstructions of camenellan scleritomes with sclerites arranged in medial and peripheral concentric zones. It further supports the conjecture that molluscs and brachiopods are descended from an ancestral vermiform and slug-like bodyplan.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41467-021-21264-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7884407PMC
February 2021

Edge reconstructions of black phosphorene: a global search.

Nanoscale 2021 Feb;13(7):4085-4091

School of Physical Science and Technology, Ningbo University, Ningbo 315211, P.R. China and State Key Lab of Superhard Materials, College of Physics, Jilin University, Changchun 130012, P. R. China.

Despite reports of possible edge reconstructions of black phosphorene, the underlying mechanisms that determine the atomic configurations and appearance of black phosphorene edges have not been elucidated to date. In this study, the particle swarm optimization (PSO) algorithm is used to perform a global search of black phosphorene edge structures. In addition to the most stable edges, three databases of the typical black phosphorene zigzag edge, armchair edge, and skewed diagonal edge are constructed for the first time. The local phosphorus concentration plays an important role in determining the edge atomic configurations and the appearance of an edge. Variations in the local phosphorus concentration result in the rearrangement of sp3-hybrid bonds or the formation of double bonds that balance the dangling bonds at the edges and stabilize the black phosphorene edges. The black phosphorene edge databases provide a useful reference for edge studies of other 2D materials with puckered honeycomb structures.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d0nr08505gDOI Listing
February 2021

Reprogramming the Tumor Microenvironment through Second-Near-Infrared-Window Photothermal Genome Editing of PD-L1 Mediated by Supramolecular Gold Nanorods for Enhanced Cancer Immunotherapy.

Adv Mater 2021 Mar 4;33(12):e2006003. Epub 2021 Feb 4.

College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, 310058, China.

A photothermal genome-editing strategy is described to improve immune checkpoint blockade (ICB) therapy by CRISPR/Cas9-mediated disruption of PD-L1 and mild-hyperthermia-induced activation of immunogenic cell death (ICD). This strategy relies on a supramolecular cationic gold nanorod that not only serves as a carrier to deliver CRISPR/Cas9 targeting PD-L1, but also harvests the second near-infrared-window (NIR-II) light and converts into mild hyperthermia to induce both ICD and gene expression of Cas9. The genomic disruption of PD-L1 significantly augments ICB therapy by improving the conversion of dendritic cells to T cells, followed by promoting the infiltration of cytotoxic T lymphocytes into tumors, thereby reprogramming immunosuppressive tumor microenvironment into immunoactive one. Such a therapeutic modality greatly inhibits the activity of primary and metastatic tumors and exhibits long-term immune memory effects against both rechallenged and recurrent tumors. The current therapeutic strategy for synergistic PD-L1 disruption and ICD activation represents an appealing way for cancer immunotherapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/adma.202006003DOI Listing
March 2021