Publications by authors named "D W Shen"

4,160 Publications

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Regulation of Fluorescence Solvatochromism To Resolve Cellular Polarity upon Protein Aggregation.

Anal Chem 2021 Dec 3. Epub 2021 Dec 3.

The Second Hospital of Dalian Medical University, 467 Zhongshan Road, Dalian, 116023, P. R. China.

Common solvatochromic fluorophores exhibit a bathochromic fluorescence emission wavelength shift accompanied by intensity attenuation due to the presence of nonradiative decay pathways at the excited state. Such intrinsic but inevitable fluorescence quenching of solvatochromism impedes its applications to faithfully quantify local polarity, especially in a polar environment. Herein, we report a new donor-π-acceptor (D-π-A) type solvatochromic fluorophore scaffold containing a perfluorophenyl group that exhibits both a solvatochromic emission wavelength shift and a controllable emission intensity upon polarity fluctuation. The regulation of fluorescence solvatochromism and colors was achieved by tuning the aryl donors. We exploited such desired solvatochromism of these probes to monitor protein misfolding and aggregation via wavelength shift. Finally, the polarity of pathogenic aggregated proteins was quantified by HaloTag bioorthogonal labeling technology in live cells. While much effort has been devoted to resolving the morphology of pathogenic aggregated proteins, this work provides quantitative hints regarding the chemical information at this disease-related protein interphase.
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http://dx.doi.org/10.1021/acs.analchem.1c03401DOI Listing
December 2021

Room-temperature multiple ligands-tailored SnO quantum dots endow in situ dual-interface binding for upscaling efficient perovskite photovoltaics with high V.

Light Sci Appl 2021 Dec 2;10(1):239. Epub 2021 Dec 2.

Department of Electronic and Information Engineering, Research Institute for Smart Energy (RISE), Guangdong-Hong Kong-Macao (GHM) Joint Laboratory for Photonic-Thermal-Electrical Energy Materials and Devices, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China.

The benchmark tin oxide (SnO) electron transporting layers (ETLs) have enabled remarkable progress in planar perovskite solar cell (PSCs). However, the energy loss is still a challenge due to the lack of "hidden interface" control. We report a novel ligand-tailored ultrafine SnO quantum dots (QDs) via a facile rapid room temperature synthesis. Importantly, the ligand-tailored SnO QDs ETL with multi-functional terminal groups in situ refines the buried interfaces with both the perovskite and transparent electrode via enhanced interface binding and perovskite passivation. These novel ETLs induce synergistic effects of physical and chemical interfacial modulation and preferred perovskite crystallization-directing, delivering reduced interface defects, suppressed non-radiative recombination and elongated charge carrier lifetime. Power conversion efficiency (PCE) of 23.02% (0.04 cm) and 21.6% (0.98 cm, V loss: 0.336 V) have been achieved for the blade-coated PSCs (1.54 eV E) with our new ETLs, representing a record for SnO based blade-coated PSCs. Moreover, a substantially enhanced PCE (V) from 20.4% (1.15 V) to 22.8% (1.24 V, 90 mV higher V, 0.04 cm device) in the blade-coated 1.61 eV PSCs system, via replacing the benchmark commercial colloidal SnO with our new ETLs.
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http://dx.doi.org/10.1038/s41377-021-00676-6DOI Listing
December 2021

Near-Infrared Light-Responsive SERS Tags Enable Positioning and Monitoring of the Drug Release of Photothermal Nanomedicines In Vivo.

Anal Chem 2021 Dec 1. Epub 2021 Dec 1.

CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China.

Understanding the in vivo behavior of photothermal nanomedicines (PTNMs) is important for drug development and evaluation. However, it is still very challenging. Herein, two key parameters, i.e., the depth of PTNMs under biological tissue and the drug release ratio of PTNMs in vivo, can be revealed by a near-infrared (NIR) light-responsive surface-enhanced Raman scattering (SERS) strategy. The fabricated PTNMs were composed of waxberry-like gold nanoparticles, model drug curcumin, and an elaborately selected NIR light-responsive Raman reporter (3,3'-diethylthiatricarbocyanine iodide, DTTC). The response mechanism of DTTC to NIR light was investigated as photodegradation. NIR light irradiation heated the gold nanoparticles, triggered the release of a model drug, and simultaneously decreased the SERS intensity of the PTNMs. In vitro experiment results revealed that the SERS intensity decrease could well reflect the depth of PTNMs with a correlation coefficient of more than 0.99. On this basis, after in situ SERS detection, the depth of PTNMs in a tumor could be revealed with satisfactory accuracy. Moreover, the decrease in the SERS intensity of PTNMs showed a highly similar trend to the increase in the drug release, suggesting that it could be used for real-time monitoring of drug release of PTNMs. This study not only opens a new avenue for the release study of many inactive fluorescent and Raman drugs of PTNMs but also provides an effective way for reporting the depth, which greatly promotes the application of PTNMs in vivo.
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http://dx.doi.org/10.1021/acs.analchem.1c03949DOI Listing
December 2021

Long-term results of alumina ceramic-on-ceramic bearings in cementless total hip arthroplasty: a 20-year minimum follow-up.

J Arthroplasty 2021 Nov 26. Epub 2021 Nov 26.

Department of Orthopaedics and Traumatic Surgery, Royal North Shore Hospital, Sydney, Australia; Institute of Bone and Joint Research, Kolling Institute, The University of Sydney, Australia.

Background: Alumina ceramic-on-ceramic (CoC) bearings are used in total hip arthroplasty (THA) due to their wear-resistant and inert properties. In this study, we assessed the clinical and radiographic outcomes of patients undergoing primary cementless CoC THA at a minimum follow-up of 20 years.

Methods: A series of 301 consecutive primary THAs in 283 patients were assessed. Clinically, patients were assessed with the modified Harris Hip Score (HHS) and pain questionnaires. Anteroposterior radiographs of the pelvis and lateral radiographs of the hip were used to radiologically assess the implant. Patients were classified as lost to follow-up if they could not be contacted on multiple occasions or did not wish to participate further in this study.

Results: At twenty years post-operation, 60 patients had died of a cause unrelated to surgery, 16 had experienced complications requiring reoperation and 100 hips had both clinical assessments and radiographs at a minimum of 20 years follow-up. The average HHS improved from 56.1 (range:17-89) pre-THA to 92.5 (range:63-100) at latest follow-up. Classification of the HHS was good or excellent in 96.4% of patients. Only 1.8% of patients still had moderate residual pain at the thigh or groin. Radiographically, all patient demonstrated bony ingrowth no clinical symptoms of loosening. The overall survival rate of the implants was 94.2% at 20 years with revision for any reason as the end point.

Conclusions: Long term follow-up in our series showed excellent implant survival, functional outcomes and minimal late complications. There was no significant radiographic evidence of failure at a minimum of 20 years post-THA.
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http://dx.doi.org/10.1016/j.arth.2021.11.028DOI Listing
November 2021

Altered Connectedness of the Brain Chronnectome During the Progression to Alzheimer's Disease.

Neuroinformatics 2021 Nov 26. Epub 2021 Nov 26.

Department of Radiology and BRIC, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.

Graph theory has been extensively used to investigate brain network topology and its changes in disease cohorts. However, many graph theoretic analysis-based brain network studies focused on the shortest paths or, more generally, cost-efficiency. In this work, we use two new concepts, connectedness and 2-connectedness, to measure different global properties compared to the previously widely adopted ones. We apply them to unravel interesting characteristics in the brain, such as redundancy design and further conduct a time-varying brain functional network analysis for characterizing the progression of Alzheimer's disease (AD). Specifically, we define different connectedness and 2-connectedness states and evaluate their dynamics in AD and its preclinical stage, mild cognitive impairment (MCI), compared to the normal controls (NC). Results indicate that, compared to MCI and NC, brain networks of AD tend to be more frequently connected at a sparse level. For MCI, we found that their brains are more likely to be 2-connected in the minimal connected state as well indicating increasing redundancy in brain connectivity. Such a redundant design could ensure maintained connectedness of the MCI's brain network in the case that pathological damages break down any link or silenced any node, making it possible to preserve cognitive abilities. Our study suggests that the redundancy in the brain functional chronnectome could be altered in the preclinical stage of AD. The findings can be successfully replicated in a retest study and with an independent MCI dataset. Characterizing redundancy design in the brain chronnectome using connectedness and 2-connectedness analysis provides a unique viewpoint for understanding disease affected brain networks.
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http://dx.doi.org/10.1007/s12021-021-09554-3DOI Listing
November 2021
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