Publications by authors named "D Neil Hayes"

2,267 Publications

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Hybrid adipose graft materials synthesized from chemically modified adipose extracellular matrix.

J Biomed Mater Res A 2021 Jul 15. Epub 2021 Jul 15.

Department of Biomedical Engineering, The Pennsylvania State University, University Park, Pennsylvania, USA.

Decellularized extracellular matrix (ECM) from tissues is a promising biomaterial that can provide a complex 3D microenvironment capable of modulating cell response and tissue regeneration. In this study, we have integrated the decellularized thiolated adipose-derived ECM, at different concentrations, with polyethylene glycol (PEG) using Michael addition between thiol and acrylate moieties. The potential for this material to support adipogenic differentiation of human adipose-derived stem cells was evaluated by encapsulating cells in hydrogels with increasing concentrations of chemically modified ECM (mECM). Our results demonstrated a positive correlation between the ECM content in the hydrogels and cell proliferation, adipogenic marker expression, and lipid formation and accumulation. Furthermore, we have shown host cell infiltration and enhanced adipogenesis in vivo after implantation. These findings support the graft as a potential alternative for adipose tissue regeneration.
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http://dx.doi.org/10.1002/jbm.a.37273DOI Listing
July 2021

Detection of high-valent iron species in alloyed oxidic cobaltates for catalysing the oxygen evolution reaction.

Nat Commun 2021 Jul 9;12(1):4218. Epub 2021 Jul 9.

Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA.

Iron alloying of oxidic cobaltate catalysts results in catalytic activity for oxygen evolution on par with Ni-Fe oxides in base but at much higher alloying compositions. Zero-field Fe Mössbauer spectroscopy and X-ray absorption spectroscopy (XAS) are able to clearly identify Fe in mixed-metal Co-Fe oxides. The highest Fe population is obtained in the 40-60% Fe alloying range, and XAS identifies the ion residing in an octahedral oxide ligand field. The oxygen evolution reaction (OER) activity, as reflected in Tafel analysis of CoFeO films in 1 M KOH, tracks the absolute concentration of Fe. The results reported herein suggest an important role for the formation of the Fe redox state in activating cobaltate OER catalysts at high iron loadings.
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http://dx.doi.org/10.1038/s41467-021-24453-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8270959PMC
July 2021

Mobile low-threshold buprenorphine integrated with infectious disease services.

J Subst Abuse Treat 2021 Jun 24:108553. Epub 2021 Jun 24.

Johns Hopkins University School of Medicine, 600 N. Wolfe St., Baltimore, MD, USA.

Introduction: In 2018, the Baltimore City Health Department launched a mobile clinic called Healthcare on The Spot, which offers low-threshold buprenorphine services integrated with health care services to meet the needs of people who use drugs. In addition to buprenorphine management, The Spot offers testing and treatment for hepatitis C, sexually transmitted infections, and HIV, as well as pre-exposure prophylaxis for HIV, wound care, vaccinations, naloxone distribution, and case management.

Methods And Materials: This cohort analysis includes clinical service data from the first 15 months of The Spot mobile clinic, from September 4, 2018, to November 23, 2019. The Spot co-located with the Baltimore syringe services program in five locations across the city. Descriptive data are provided for patient demographics and services provided, as well as percent of patients retained in buprenorphine treatment at one and three months. Logistic regression identified factors associated with retention at three months.

Results: The Spot mobile clinic provided services to 569 individuals from September 4, 2018, to November 23, 2019, including prescribing buprenorphine to 73.8% and testing to more than 70% for at least one infectious disease. Patients receiving a prescription for buprenorphine were more likely to be tested for HIV, hepatitis C, and sexually transmitted infections, as well as receive treatment for hepatitis C and preventive services including vaccination and naloxone distribution. The Spot initiated HIV treatment for four patients and HIV pre-exposure prophylaxis for twelve patients. More than 32% of patients had hepatitis C; nineteen of these patients initiated treatment for hepatitis C with eight having a documented cure. Buprenorphine treatment retention was 56.0% at one month and 26.2% at three months. Patients who were Black or receiving treatment for hepatitis C were more likely to be retained in buprenorphine treatment at three months.

Conclusions: Increasing access to integrated medical services and drug treatment through low-threshold, community-based models of care can be an effective tool for addressing the effects of drug use.
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http://dx.doi.org/10.1016/j.jsat.2021.108553DOI Listing
June 2021

Systematic analysis of SARS-CoV-2 infection of an ACE2-negative human airway cell.

Cell Rep 2021 07 23;36(2):109364. Epub 2021 Jun 23.

Department of Cell Biology and Physiology, Washington University in St. Louis, School of Medicine, St. Louis, MO, USA; Department of Otolaryngology, Washington University in St. Louis, School of Medicine, St. Louis, MO, USA. Electronic address:

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S) variants govern transmissibility, responsiveness to vaccination, and disease severity. In a screen for new models of SARS-CoV-2 infection, we identify human H522 lung adenocarcinoma cells as naturally permissive to SARS-CoV-2 infection despite complete absence of angiotensin-converting enzyme 2 (ACE2) expression. Remarkably, H522 infection requires the E484D S variant; viruses expressing wild-type S are not infectious. Anti-S monoclonal antibodies differentially neutralize SARS-CoV-2 E484D S in H522 cells as compared to ACE2-expressing cells. Sera from vaccinated individuals block this alternative entry mechanism, whereas convalescent sera are less effective. Although the H522 receptor remains unknown, depletion of surface heparan sulfates block H522 infection. Temporally resolved transcriptomic and proteomic profiling reveal alterations in cell cycle and the antiviral host cell response, including MDA5-dependent activation of type I interferon signaling. These findings establish an alternative SARS-CoV-2 host cell receptor for the E484D SARS-CoV-2 variant, which may impact tropism of SARS-CoV-2 and consequently human disease pathogenesis.
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http://dx.doi.org/10.1016/j.celrep.2021.109364DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8220945PMC
July 2021

Outcomes of primary radiotherapy with or without chemotherapy for advanced oral cavity squamous cell carcinoma: A systematic review.

Head Neck 2021 Jun 24. Epub 2021 Jun 24.

Department of Otolaryngology, The University of Tennessee Health Science Center, Memphis, Tennessee, USA.

Background: Surgery with adjuvant radiotherapy is the accepted standard for treatment of advanced oral cavity squamous cell carcinoma (OCSCC); however, alternative evidence suggests that definitive (chemo)radiotherapy may have similar outcomes.

Methods: Systematic review was performed to assess the therapeutic value of radiotherapy or chemoradiotherapy as a primary modality for treating OCSCC. Meta-analysis of outcomes was performed between articles comparing radiotherapy and primary surgical treatment.

Results: Meta-analysis showed less favorable results of radiotherapy compared to surgery: overall survival at 3-years (odds ratio [OR] = 0.51; 95% confidence interval [CI] = 0.34-0.77) and 5-years (OR = 0.42; 95% CI = 0.29-0.60); disease-specific survival at 3-years (OR = 0.55; 95% CI = 0.32-0.96) and 5-years (OR = 0.55; 95% CI = 0.32-0.96). Odds of feeding tube dependency were higher in primary radiotherapy group (OR = 2.67; 95% CI = 1.27-5.64).

Conclusions: Results of this study support the current perspective favoring primary surgical treatment for OCSCC in the absence of surgical contraindications.
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http://dx.doi.org/10.1002/hed.26779DOI Listing
June 2021
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