Publications by authors named "D I Chebotarev"

70 Publications

[The prognostic value of ASXL1 mutation in primary myelofibrosis. Literature review and clinical case description].

Ter Arkh 2020 Sep 1;92(7):95-99. Epub 2020 Sep 1.

National Research Center for Hematology.

Primary myelofibrosis is a myeloproliferative neoplasm that occurs de novo, characterized by clonal proliferation of stem cells, abnormal expression of cytokines, bone marrow fibrosis, hepatosplenomegaly as a result of extramedullary hematopoiesis, symptoms of tumor intoxication, cachexemia, peripheral blood leukoerythroblastosis, leukemic progression and low survival. Primary myelofibrosis is a chronic incurable disease. The aims of therapy: preventing progression, increasing overall survival, improving quality of life. The choice of therapeutic tactics is limited. Allogenic hematopoietic stem cell transplantation is the only method that gives a chance for a cure. The role of mutations in a number of genes in the early identification of candidates for allogeneic hematopoietic stem cell transplantation is being actively studied. The article describes the clinical case of the detection ofASXL1gene mutations in a patient with prefibrous primary myelofibrosis. The diagnosis was established on the basis of WHO criteria 2016. The examination revealed a mutation ofASXL1. Interferon alfa therapy is carried out, against the background of which clinico-hematological remission has been achieved. Despite the identified mutation, the patient is not a candidate for allogeneic hematopoietic stem cell transplantation. Given the unfavorable prognostic value of theASXL1mutation, the patient is subject to active dynamic observation and aggressive therapeutic tactics when signs of disease progression appear.
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http://dx.doi.org/10.26442/00403660.2020.07.000788DOI Listing
September 2020

Gray-zone lymphoma. Examples of rare clinical manifestation.

Ter Arkh 2019 May;91(4):107-113

National Research Center for Hematology of the Ministry of Health of the Russian Federation, Moscow, Russia.

Mediastinal gray-zone lymphoma (MGZL, lymphoma with features intermediate between classical Hodgkin lymphoma and diffuse large B-cell lymphoma) was declared as a separate entity in WHO classification of Tumors of Haematopoetic and Lymphoid Tissues in 2008 and 2017 years. Despite of similar pathomorphological characteristics between primary mediastinal B-cell lymphoma and Hodgkin lymphoma, clinical features and optimal therapeutic approach to MGZL are not clearly defined. Usually MGZL manifests with mediastinal lymphadenopathy, although extranodal lesions often occur (grey-zone lymphoma, GZL). Patients with MGZL have unfavorable prognosis, taking into account high rate of relapse. This article describes two cases of MGZL. First case manifested by arrhythmias due to primary heart involvement. In spite of cardiac failure antracycline-containing chemotherapy (6 courses of R-DA-EPOCH) it allowed to achieve a complete remission and resolving of arrhythmias. Second case was represented by metachronous tumors: primary mediastinal B-cell lymphoma at the time of disease onset and classical Hodgkin lymphoma, NS II, diagnosed after disease progression. Thus, we demonstrated two examples of MGZL that differ by clinical manifestation, response to chemotherapy, which emphasizes an importance of pathogenesis studying, and using of new therapeutic approaches.
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http://dx.doi.org/10.26442/00403660.2019.04.000071DOI Listing
May 2019

[Termination of Replication and Mechanisms of Heteroplasmy in Sturgeon Mitochondrial DNA].

Mol Biol (Mosk) 2019 Jan-Feb;53(1):53-63

Southern Scientific Center of the Russian Academy of Sciences, Rostov-on-Don, 344006 Russia.

The control region of mitochondrial DNA (mtDNA) in sturgeon contains one to seven tandem nucleotide repeats 78-83 bp in size. Some sturgeon species are homoplasmic by the D-loop size (Acipenser nudiventris, A. oxyrinchus, A. sturio), some are mildly heteroplasmic (A. fulvescens, Huso huso) and some are markedly heteroplasmic (A. brevirostrum, A. medirostris, A. mikadoi, A. naccarii, and A. transmontanus). This work presents a comparison of the D-loop sequences associated with the termination of mtDNA replication in fish and the conservative sequences determining the termination of replication (TAS) in these organisms. It is proposed that the D-loop heteroplasmy in sturgeon may be associated with variation in the number of tandem repeat sequences, which can form stable spatial structures during mtDNA replication. In most sturgeon species with pronounced heteroplasmy, the energy levels required for the folding of tandem repeats containing variable number of repeated units differ minimally.
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http://dx.doi.org/10.1134/S0026898419010063DOI Listing
August 2019

[Features of redox processes in the amniotic fluid at placental insufficiency].

Biomed Khim 2018 Jun;64(3):290-297

Federal Research Centre Southern Scientific Center of Russian Academy of Sciences, Rostov-on-Don, Russia.

Activity of prooxidant enzymes (NADPH-oxidase and xanthine oxidase), antioxidant enzymes (superoxide dismutase (SOD) and catalase), enzymes of the glutathione-dependent systems, as well as antioxidant vitamins (retinol and a-tocopherol), lipid peroxidation products (LPP) (conjugated dienes and Schiff bases), and peroxide chemiluminescence were studied in the amniotic fluid at different periods of physiological pregnancy and placental insufficiency (PI). It was found that at PI the activity of NADPH-oxidase, xanthine oxidase increased and the activity of SOD, catalase, glutathione peroxidase, glutathione reductase, glutathione transferase and the content of fat-soluble vitamins decreased. The direct and inverse correlation between the studied pro- and antioxidant parameters and the content of LPP products, was found ro be different in the II and III trimesters of gestation. The revealed differences obviously reflect metabolic impairments in the fetoplacental complex, and the activity and level of the paremeters of redox processes can be used as tests for pre- and postnatal disorders.
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http://dx.doi.org/10.18097/PBMC20186403290DOI Listing
June 2018

MicroRNA-Dependent Regulation of IGF1R Gene Expression in Hormone-Sensitive and Hormone-Resistant Prostate Cancer Cells.

Dokl Biochem Biophys 2018 Mar 19;479(1):101-104. Epub 2018 May 19.

Southern Scientific Center, Russian Academy of Sciences, Rostov-on-Don, 3444006, Russia.

Using multiple parallel sequencing on Illumina platform, we identified eight microRNAs that showed significant opposite changes of gene expression in cells of the hormone-sensitive LNCaP prostate cancer cell line and in cells of the hormone-resistant DU-145 cell line, in comparison to the microRNA expression in the normal prostate tissue cells. We found that the insulin-like growth factor 1 receptor (IGF1R) gene is a target of five microRNAs whose expression is increased in LNCaP cells and reduced in DU-145 cells.
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http://dx.doi.org/10.1134/S1607672918020138DOI Listing
March 2018