Publications by authors named "D B Barber"

1,107 Publications

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Iron oxide and iron oxyhydroxide nanoparticles impair SARS-CoV-2 infection of cultured cells.

J Nanobiotechnology 2022 Jul 30;20(1):352. Epub 2022 Jul 30.

Department of Immunology, Oncology and Nanobiomedicine Initiative, Centro Nacional de Biotecnología (CNB-CSIC), Darwin 3, 28049, Madrid, Spain.

Background: Coronaviruses usually cause mild respiratory disease in humans but as seen recently, some human coronaviruses can cause more severe diseases, such as the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), the global spread of which has resulted in the ongoing coronavirus pandemic.

Results: In this study we analyzed the potential of using iron oxide nanoparticles (IONPs) coated with biocompatible molecules like dimercaptosuccinic acid (DMSA), 3-aminopropyl triethoxysilane (APS) or carboxydextran (FeraSpin™ R), as well as iron oxyhydroxide nanoparticles (IOHNPs) coated with sucrose (Venofer), or iron salts (ferric ammonium citrate -FAC), to treat and/or prevent SARS-CoV-2 infection. At non-cytotoxic doses, IONPs and IOHNPs impaired virus replication and transcription, and the production of infectious viruses in vitro, either when the cells were treated prior to or after infection, although with different efficiencies. Moreover, our data suggest that SARS-CoV-2 infection affects the expression of genes involved in cellular iron metabolism. Furthermore, the treatment of cells with IONPs and IOHNPs affects oxidative stress and iron metabolism to different extents, likely influencing virus replication and production. Interestingly, some of the nanoparticles used in this work have already been approved for their use in humans as anti-anemic treatments, such as the IOHNP Venofer, and as contrast agents for magnetic resonance imaging in small animals like mice, such as the FeraSpin™ R IONP.

Conclusions: Therefore, our results suggest that IONPs and IOHNPs may be repurposed to be used as prophylactic or therapeutic treatments in order to combat SARS-CoV-2 infection.
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http://dx.doi.org/10.1186/s12951-022-01542-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9338509PMC
July 2022

Rapid GPR183-mediated recruitment of eosinophils to the lung after Mycobacterium tuberculosis infection.

Cell Rep 2022 Jul;40(4):111144

Human Eosinophil Section, Laboratory of Parasitic Diseases, NIAID, NIH, Bethesda, MD 20892, USA.

Influx of eosinophils into the lungs is typically associated with type II responses during allergy and fungal and parasitic infections. However, we previously reported that eosinophils accumulate in lung lesions during type I inflammatory responses to Mycobacterium tuberculosis (Mtb) in humans, macaques, and mice, in which they support host resistance. Here we show eosinophils migrate into the lungs of macaques and mice as early as one week after Mtb exposure. In mice this influx is CCR3 independent and instead requires cell-intrinsic expression of the oxysterol receptor GPR183, which is highly expressed on human and macaque eosinophils. Murine eosinophils interact directly with bacilli-laden alveolar macrophages, which upregulate the oxysterol-synthesizing enzyme Ch25h, and eosinophil recruitment is impaired in Ch25h-deficient mice. Our findings show that eosinophils are among the earliest cells from circulation to sense and respond to Mtb infection of alveolar macrophages and reveal a role for GPR183 in the migration of eosinophils into lung tissue.
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http://dx.doi.org/10.1016/j.celrep.2022.111144DOI Listing
July 2022

Development of a Novel Targeted Metabolomic LC-QqQ-MS Method in Allergic Inflammation.

Metabolites 2022 Jun 25;12(7). Epub 2022 Jun 25.

Department of Basic Medical Sciences, Facultad de Medicina, Institute of Applied Molecular Medicine (IMMA), Universidad San Pablo CEU, CEU Universities, Urbanización Montepríncipe, 28003 Madrid, Spain.

The transition from mild to severe allergic phenotypes is still poorly understood and there is an urgent need of incorporating new therapies, accompanied by personalized diagnosis approaches. This work presents the development of a novel targeted metabolomic methodology for the analysis of 36 metabolites related to allergic inflammation, including mostly sphingolipids, lysophospholipids, amino acids, and those of energy metabolism previously identified in non-targeted studies. The methodology consisted of two complementary chromatography methods, HILIC and reversed-phase. These were developed using liquid chromatography, coupled to triple quadrupole mass spectrometry (LC-QqQ-MS) in dynamic multiple reaction monitoring (dMRM) acquisition mode and were validated using ICH guidelines. Serum samples from two clinical models of allergic asthma patients were used for method application, which were as follows: (1) corticosteroid-controlled (ICS, = 6) versus uncontrolled (UC, = 4) patients, and immunotherapy-controlled (IT, = 23) versus biologicals-controlled (BIO, = 12) patients. The results showed significant differences mainly in lysophospholipids using univariate analyses in both models. Multivariate analysis for model 1 was able to distinguish both groups, while for model 2, the results showed the correct classification of all BIO samples within their group. Thus, this methodology can be of great importance for further understanding the role of these metabolites in allergic diseases as potential biomarkers for disease severity and for predicting patient treatment response.
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http://dx.doi.org/10.3390/metabo12070592DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9319984PMC
June 2022

Comparison of Nutritive Values of Tropical Pasture Species Grown in Different Environments, and Implications for Livestock Methane Production: A Meta-Analysis.

Animals (Basel) 2022 Jul 14;12(14). Epub 2022 Jul 14.

Agri-Science Queensland, Department of Agriculture and Fisheries Queensland, University of Queensland Gatton Campus, Lawes, QLD 4343, Australia.

The demand for dairy products is ever increasing across the world. The livestock sector is a significant source of greenhouse gas (GHG) emissions globally. The availability of high-quality pasture is a key requirement to increase the productivity of dairy cows as well as manage enteric methane emissions. Warm-season perennial grasses are the dominant forages in tropical and subtropical regions, and thus exploring their nutritive characteristics is imperative in the effort to improve dairy productivity. Therefore, we have collated a database containing a total of 4750 records, with 1277 measurements of nutritive values representing 56 tropical pasture species and hybrid cultivars grown in 26 different locations in 16 countries; this was done in order to compare the nutritive values and GHG production across different forage species, climatic zones, and defoliation management regimes. Average edaphoclimatic (with minimum and maximum values) conditions for tropical pasture species growing environments were characterized as 22.5 °C temperature (range 17.5-29.30 °C), 1253.9 mm rainfall (range 104.5-3390.0 mm), 582.6 m elevation (range 15-2393 m), and a soil pH of 5.6 (range 4.6-7.0). The data revealed spatial variability in nutritive metrics across bioclimatic zones and between and within species. The ranges of these nutrients were as follows: neutral detergent fibre (NDF) 50.9-79.8%, acid detergent fibre (ADF) 24.7-57.4%, crude protein (CP) 2.1-21.1%, dry matter (DM) digestibility 30.2-70.1%, metabolisable energy (ME)3.4-9.7 MJ kg DM, with methane (CH) production at 132.9-133.3 g animal day. The arid/dry zone recorded the highest DM yield, with decreased CP and high fibre components and minerals. Furthermore, the data revealed that climate, defoliation frequency and intensity, in addition to their interactions, have a significant effect on tropical pasture nutritive values and CH production. Overall, hybrid and newer tropical cultivars performed well across different climates, with small variations in herbage quality. The current study revealed important factors that affect pasture nutritive values and CH emissions, with the potential for improving tropical forage through the selection and management of pasture species.
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http://dx.doi.org/10.3390/ani12141806DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9311783PMC
July 2022

Mast Cell Desensitization in Allergen Immunotherapy.

Front Allergy 2022 16;3:898494. Epub 2022 Jun 16.

Department of Basic Medical Sciences, Facultad de Medicina, Institute of Applied Molecular Medicine Nemesio Díez, Universidad San Pablo-CEU, CEU Universities, Urbanización Montepríncipe, Madrid, Spain.

Allergen immunotherapy (AIT) is the only treatment with disease-transforming potential for allergic disorders. The immunological mechanisms associated with AIT can be divided along time in two phases: short-term, involving mast cell (MC) desensitization; and long-term, with a regulatory T cell (Treg) response with significant reduction of eosinophilia. This regulatory response is induced in about 70% of patients and lasts up to 3 years after AIT cessation. MC desensitization is characteristic of the initial phase of AIT and it is often related to its success. Yet, the molecular mechanisms involved in allergen-specific MC desensitization, or the connection between MC desensitization and the development of a Treg arm, are poorly understood. The major AIT challenges are its long duration, the development of allergic reactions during AIT, and the lack of efficacy in a considerable proportion of patients. Therefore, reaching a better understanding of the immunology of AIT will help to tackle these short-comings and, particularly, to predict responder-patients. In this regard, omics strategies are empowering the identification of predictive and follow-up biomarkers in AIT. Here, we review the immunological mechanisms underlying AIT with a focus on MC desensitization and AIT-induced adverse reactions. Also, we discuss the identification of novel biomarkers with predictive potential that could improve the rational use of AIT.
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http://dx.doi.org/10.3389/falgy.2022.898494DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9278139PMC
June 2022
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