Publications by authors named "Cyril Habougit"

21 Publications

  • Page 1 of 1

Histopathological typing in diffuse malignant epithelioid mesothelioma: implication for prognosis and molecular basis.

Pathology 2021 May 6. Epub 2021 May 6.

University Hospital of Saint Etienne, North Hospital, Department of Pathology, Saint Etienne, France; Corneal Graft Biology, Engineering, and Imaging Laboratory, BiiGC, EA2521, Federative Institute of Research in Sciences and Health Engineering, Faculty of Medicine, Jean Monnet University, Saint-Etienne, France.

The prognostic impact of tumour grading, cytological and architectural patterns and stromal features in diffuse pleural malignant epithelioid mesothelioma (MEM) has been partly studied but not correlated to molecular features. We performed a retrospective study on 92 MEM in our department in order to assess the prognostic role of architectural and stromal patterns, especially tumour to stroma ratio. Secondly, based on The Cancer Genome Atlas (TCGA) database, we analysed the differentially expressed genes in prognostic groups of interest. Our results showed that tumour grading, tumour to stroma ratio and predominant pattern were related to overall survival, p≤0.001, p=0.01 and p=0.001, respectively. In univariate analysis, for high grade tumours hazard ratio (HR) was 4.75 (2.47-9.16), for stroma poor tumours HR=0.016, for predominant tubular or tubulopapillary pattern HR=0.044. In multivariate analysis, high grade tumours were related to overall survival [HR=3.09 (1.50-6.35), p=0.002] and predominant tubular or tubulopapillary pattern [HR=0.56 (0.32-0.99), p=0.045]. In TCGA analysis, after grading of diagnostic slides, we showed that KRTDAP and CXRCR1 expression was higher in low grade tumours, unlike PDZD7 and GPR176 expression which was higher in high grade tumours. FAM81B had a higher expression in stroma poor tumours. We did not find any differentially expressed genes in the architectural patterns group. Our work suggests that tumour grading is an important parameter in MEM with an underlying genomic basis. The role of tumour to stroma ratio needs to be investigated and might also have a genomic basis.
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http://dx.doi.org/10.1016/j.pathol.2021.01.010DOI Listing
May 2021

Line field confocal optical coherence tomography: An adjunctive tool in the diagnosis of autoimmune bullous diseases.

J Biophotonics 2021 May 22;14(5):e202000449. Epub 2021 Feb 22.

Dermatology Unit and Skin Bank, Department of Medical, Surgical and Neurosciences, Siena University Hospital, Siena, Italy.

Autoimmune bullous diseases (AIBDs) still represent a considerable a source of morbidity and mortality: early identification of a specific AIBD is often difficult due to overlapping clinical and/or laboratory features and time-consuming invasive laboratory tests. We aimed to investigate the potential role of a new imaging technology, line-field confocal optical coherence tomography (LC-OCT), in the non-invasive diagnosis of AIBDs. LC-OCT was performed at lesional, perilesional and contralateral healthy sites in 30 patients, before histology and direct immunofluorescence. LC-OCT examination was able to identify the level of split (subcorneal/suprabasal/subepidermal/sublamina densa), to provide detailed images of the bulla roof morphology and content (eg, erythrocytes/acantholytic cells/polymorphonucleates). Areas of intra/subepidermal detachment were also detected also at clinically normal perilesional skin sites. LC-OCT can support physicians, real time and at bed-site, in the differential diagnosis of various AIBDs and their mimickers. Moreover, it can be used for the identification of subclinical lesions and therapy tapering.
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http://dx.doi.org/10.1002/jbio.202000449DOI Listing
May 2021

Squamous cell carcinoma arising on acrodermatitis continua of Hallopeau: clinical and noninvasive skin imaging features.

Int J Dermatol 2021 Jun 25;60(6):763-765. Epub 2020 Nov 25.

Department of Medical, Surgical and Neurological Science, Dermatology Section, University of Siena, S. Maria alle Scotte Hospital, Siena, Italy.

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http://dx.doi.org/10.1111/ijd.15297DOI Listing
June 2021

Systemic sarcoidosis revealed by venepunctures: a very rare but rewarding cutaneous manifestation.

BMJ Case Rep 2020 Sep 7;13(9). Epub 2020 Sep 7.

Department of Internal Medicine, Centre Hospitalier Universitaire de Saint-Etienne, Saint-Etienne, Loire, France.

A 67-year-old man was referred to our department for the onset of cutaneous lesions following venepunctures. His recent medical history included brief flu-like syndrome, persistent cough, dyspnoea, dry mouth, blurred vision and weight loss. The extensive clinical, biological and radiological check-up showed signs consistent with systemic sarcoidosis: right uveitis, hypercalcemia, renal failure, inflammatory syndrome, elevated levels of ACE, alveolitis with elevated CD4+/CD8+ T cell ratio, hilar and mediastinal lymphadenopathy, bilateral pulmonary infiltrates, mild bronchial obstruction and lowered diffusing capacity of the lungs for carbon monoxide. Multiple biopsy samples (bronchus, accessory salivary glands and one of the skin lesions) eventually confirmed the diagnosis. Corticosteroids resulted in skin lesions resolution in a few days and overall clinical, biological and lung function improvement. The infiltration of scars by granulomatous tissue is well recognised in sarcoidosis but its onset in venepuncture sites is a very rare but easily recognisable condition, which can be helpful for quick diagnosis purpose.
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http://dx.doi.org/10.1136/bcr-2020-235784DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7477996PMC
September 2020

Multicystic and diffuse malignant peritoneal mesothelioma in children.

Pediatr Blood Cancer 2020 06 11;67(6):e28286. Epub 2020 Apr 11.

Department of Pediatric Surgery, University Hospital of Saint-Etienne, Saint-Priest-en-Jarez, France.

Background: Malignant and multicystic peritoneal mesotheliomas are extremely rare tumors in children, developing from mesothelial cells. No specific guidelines are available at this age.

Methods: We performed a retrospective analysis of all identified children (< 18-year-old) treated in France from 1987 to 2017 for a diffuse malignant peritoneal mesothelioma (DMPM) or a multicystic peritoneal mesothelioma (MCPM).

Results: Fourteen patients (5 males and nine females), aged 2.2 to 17.5 years, were included. The most frequent presenting symptoms were abdominal pain, ascitis, and alteration in the general condition. Eight patients had epithelioid mesothelioma, three had biphasic mesothelioma, and three had MCPM. Eight patients with DMPM diagnosis received cytoreductive surgery (CRS) with hyperthermic intraperitoneal chemotherapy (HIPEC). Among them, six patients had neoadjuvant systemic chemotherapy, one patient, post-operative chemotherapy, and one patient CRS and HIPEC only. Three patients received only systemic chemotherapy. All patients with MCPM had only surgery. After a median follow-up of seven years (2-15), six patients (6/11; one death) with DMPM and two patients (two/three) with MCPM had a local and distant recurrences.

Conclusion: Peritoneal mesothelioma in children is a rare condition with difficult diagnosis and high risk of recurrence. Worldwide interdisciplinary collaboration and networking are mandatory to help diagnosis and provide harmonious treatment guidelines.
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http://dx.doi.org/10.1002/pbc.28286DOI Listing
June 2020

Ex vivo confocal microscopy for dermatofibrosarcoma protuberans.

Skin Res Technol 2019 07 12;25(4):589-591. Epub 2019 Mar 12.

Department of Dermatology, University Hospital of Saint-Etienne, Saint-Etienne, France.

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http://dx.doi.org/10.1111/srt.12690DOI Listing
July 2019

Dermoscopic and reflectance microscopy features of primary and metastatic Merkel cell carcinoma: Ten cases.

Skin Res Technol 2019 May 28;25(3):407-409. Epub 2018 Dec 28.

Department of Medical, Surgical and Neurological Science - Dermatology Section, University of Siena, S. Maria alle Scotte Hospital, Siena, Italy.

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http://dx.doi.org/10.1111/srt.12658DOI Listing
May 2019

First Characterization with Ultrasound Contrast Agent of a Fibrovascular Polyp Before Its Endoscopic Resection: A Case Report (with Videos).

Clin Endosc 2019 Mar 5;52(2):186-190. Epub 2018 Oct 5.

Department of Hepatogastroenterology, University Hospital of Saint-Etienne, Saint-Priest en Jarez, France.

We described for the first time the contrast enhancement of a giant fibrovascular esophageal polyp using ultrasound contrast agent, Sonovue® (Bracco, Milan, Italy) during echoendoscopy. Fine Doppler was unsuccessful in showing vascularization due to the mobile characteristic of the tumor. In contrast, via Sonovue® , tissue microcirculation was highlighted inside the entire head of the polyp, leading to better appreciate the risk of bleeding related to its resection. In a second part, we showed the feasibility of classic polypectomy for this giant polyp (5×5 cm) without complication and results of control endoscopy at 3 months. The present case is summarized in a video.
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http://dx.doi.org/10.5946/ce.2018.083DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6453852PMC
March 2019

Nuclear grading, BAP1, mesothelin and PD-L1 expression in malignant pleural mesothelioma: prognostic implications.

Pathology 2018 Oct 23;50(6):635-641. Epub 2018 Aug 23.

University Hospital of Saint Etienne, North Hospital, Department of Pathology, Saint Etienne, France.

For malignant pleural mesothelioma (MPM), histopathological subtype is one of the most important prognostic factors. Several immunohistochemical stains whose expressions have possible therapeutic implications have been identified in MPM such as BAP1, mesothelin and PD-L1. The aim of our work was to evaluate the clinical significance and prognostic implications of BAP1, mesothelin and PD-L1 expression in 117 patients with a diagnosis of MPM who were diagnosed in our institution between 2002 and 2017. We also correlated this immunohistochemical profile to a recently described nuclear grading and to histopathological subtype. Mesothelin expression, BAP1 loss and PD-L1 expression were associated with histopathological subtype (p < 0.0001), BAP1 loss was more frequent in epithelioid subtype whereas PD-L1 expression was more frequent in non-epithelioid subtype. For epithelioid MPM, BAP1 expression was associated with overall survival (p = 0.034), with a longer survival when BAP1 expression is lost. Necrosis and nuclear grading are associated with overall survival (p = 0.0048 and <0.0001, respectively), with longer survival when necrosis was absent and for grade I. For non-epithelioid MPM, overall survival was not related to clinical, histopathological or immunohistochemical expression of BAP1, mesothelin or PD-L1. In multivariate analysis, grade I for nuclear grading was an independent prognostic factor associated with overall survival (p < 0.0001). In epithelioid and non-epithelioid MPM, we analysed overall survival in subgroups with combined mesothelin, BAP1 and PD-L1 expression. In epithelioid MPM, BAP1 retained/mesothelin negativity/PD-L1 > 1%, and BAP1 retained/mesothelin positivity/PD-L1 > 1% profiles, are associated with shorter overall survival. In non-epithelioid MPM, BAP1 loss/mesothelin negativity/PD-L1 > 1% is associated with shorter overall survival. Our work confirms that nuclear grading in epithelioid MPM is a strong and independent prognosis factor. Moreover, this study on several promising immunohistochemical stains whose expressions have possible therapeutic implications identifies subgroups with a poor prognosis.
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http://dx.doi.org/10.1016/j.pathol.2018.05.002DOI Listing
October 2018

Expression of CD3, PD-L1 and CTLA-4 in mammary and extra-mammary Paget disease.

Cancer Immunol Immunother 2018 08 25;67(8):1297-1303. Epub 2018 Jun 25.

Department of Pathology, North Hospital, University Hospital of Saint-Etienne, 42055, Cedex 2, Saint-Étienne, France.

Background: Mammary and extra-mammary Paget disease is a rare form of intra-epithelial glandular neoplasm which is characteristically recurrent and necessitates multiple excisions that have an important impact on morbidity. Local immuno-modulating treatments have been applied with promising results, but the local immune markers of Paget disease have not been studied.

Aim Of The Study: To investigate the local immune micro-environment of Paget disease.

Materials And Methods: Sixty-four specimens from 41 patients, including cases with multiple recurrences and underlying primary neoplasm, have been studied for their expression of CD3, PD-L1 and CTLA-4.

Results: Nineteen cases were mammary; 22 were extra-mammary and involved the vulva, the anus, the inguinal region and the lower extremity. PD-L1 was not expressed by any neoplastic lesion or the associated lymphocytes. CTLA-4 expression was found in nine cases. Higher stromal CD3 expression and moderate levels of intra-epithelial CD3 expression were present in most cases. Biopsies, subsequent excision specimens and recurrences showed the same immunohistochemical profile of CD3 and PD-L1, although there were different levels of CTLA-4 in a few cases. The underlying lesions in mammary Paget disease showed the same immunohistochemical profile as the intra-epithelial neoplastic cells. The expression of the markers did not correlate with age, sex, localization or recurrence.

Conclusion: Paget disease is characterized by an intense lymphocytic response, devoid of the immune-suppressive impact of the PD-L1 pathway, but with occasional CTLA-4 expression.
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http://dx.doi.org/10.1007/s00262-018-2189-xDOI Listing
August 2018

Dermoscopic and reflectance confocal microscopy features of two cases of vulvar basal cell carcinoma.

Dermatol Pract Concept 2018 Jan 31;8(1):68-71. Epub 2018 Jan 31.

Department of Medical, Surgical and Neuro-Sciences, Dermatology Unit, University of Siena, Siena, Italy.

Basal cell carcinoma (BCC) is the most common malignant skin cancer. Its genital localization is rare, and the diagnosis in this site could be challenging. Here, we report two patients with vulvar BCC and describe their clinical, dermoscopic and in vivo and ex vivo reflectance confocal microscopic (RCM) features. Dermoscopy and RCM can be useful tools for helping the clinical diagnosis of vulvar BCC and for identifying the correct surgical margins.
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http://dx.doi.org/10.5826/dpc.0801a17DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5808379PMC
January 2018

Linear variant of large plaque-type blue naevus with subcutaneous cellular nodules.

Pathology 2017 Aug 30;49(5):542-544. Epub 2017 Jun 30.

Department of Biopathology, Centre Leon Berard, Lyon, France. Electronic address:

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http://dx.doi.org/10.1016/j.pathol.2017.03.013DOI Listing
August 2017

AN 11-Year-Old Boy with a Leptomeningeal Tumor.

Brain Pathol 2017 07;27(4):553-554

Department of Pathology, North Hospital, Avenue Albert Raimond, 42055 Saint Etienne CEDEX 2, France.

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http://dx.doi.org/10.1111/bpa.12527DOI Listing
July 2017

Histopathologic features predict survival in diffuse pleural malignant mesothelioma on pleural biopsies.

Virchows Arch 2017 Jun 27;470(6):639-646. Epub 2017 Mar 27.

Department of Pathology, North Hospital, University Hospital of Saint Etienne, Avenue Albert Raimond, Cedex 2, 42055, Saint Etienne, France.

Malignant pleural mesothelioma is a rare tumor with a poor prognosis. The only universally recognized pathological prognostic factor is histopathological subtype with a shorter survival in non-epithelioid subtypes. Recently, a grading of epithelioid mesothelioma on surgical resection has been proposed. The aim of our work is to assess the prognostic role of several histopathological factors on a retrospective cohort of 116 patients diagnosed as a pleural mesothelioma for more than 95% of patients on pleural biopsy. Our work shows that mitotic count <3/10 HPF (p < 0.0001), the lack of necrosis (p = 0.0379), mild nuclear atypia (p = 0.0054), the lack of atypical mitoses (p = 0.0265), a nucleoli size <3 μm (p = 0.0139), and a nucleoli absent or visible at 200× or higher magnification (p = 0.0170) are significantly associated with a better median overall survival in epithelioid mesothelioma. The presence of atypical mitoses was found to be related to a worse median survival in non-epithelioid mesothelioma. Mitotic count, necrosis, nuclear atypia, and nucleoli size are not associated with overall survival in non-epithelioid mesothelioma. Our work highlights that histopathological prognostic factors can be assessed on pleural biopsies and can predict reliably median overall survival. This is of interest in order to define subgroups of patients who could benefit of different therapies and select patients who could benefit of surgical excision.
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http://dx.doi.org/10.1007/s00428-017-2109-zDOI Listing
June 2017

Seromucinous ovarian tumor A comparison with the rest of ovarian epithelial tumors.

Ann Diagn Pathol 2017 Apr 7;27:28-33. Epub 2017 Jan 7.

Department of Pathology, North Hospital, University Hospital of St-Etienne, France.

Background: Seromucinous ovarian tumors are rare and not adequately described in the literature and this is especially true for seromucinous carcinomas.

Aim Of The Study: To describe histological and clinical features of these tumors in comparison with the rest of ovarian epithelial tumors.

Materials And Methods: Two hundred and forty one (241) ovarian tumors, borderline (n=92) or malignant (n=149), treated surgically without neoadjuvant chemotherapy, were examined.

Results: Seromucinous borderline (SMBT) and malignant tumors (SMC) comprised 7.8% (n=7) and 4% (n=6) of all borderline tumors and carcinomas, respectively, studied. Mean age of diagnosis was 63.2 and 68.3years and mean size was 6.4cm and 12cm for SMBT and SMC, respectively. Seromucinous tumors were associated with endometriosis in 23.1% of the cases and they were bilateral in 30.8%. Microscopically, variety in cellular composition, papillary architecture and development into thick walled, occasionally muscular, cysts were the main findings. Medullary/paraovarian/tubal or deeply cortical localization was also characteristic. Stage predicted overall and progression-free survival (p<0.0001 and p=0.03, respectively). Five-year survival was 62% for patients with high grade serous carcinoma, 55% for seromucinous carcinoma, 100% for endometrioid carcinoma, 75% for clear cell carcinoma, and 80% for patients with mucinous carcinoma. Differences were not however statistically significant.

Conclusion: Seromucinous tumors have unique features that support their classification as a different entity. Their localization and their often thick fibrous or/and muscular wall provides further evidence for an histogenesis from the secondary Müllerian system or vestigial structures.
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http://dx.doi.org/10.1016/j.anndiagpath.2017.01.002DOI Listing
April 2017

The eight year evolution of an osseous PEComa.

Pathol Int 2017 03 9;67(3):181-182. Epub 2017 Jan 9.

Department of Pathology, North Hospital, University Hospital of St-Etienne, France.

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http://dx.doi.org/10.1111/pin.12500DOI Listing
March 2017

[Multicystic lesion of the peritoneum in a child].

Ann Pathol 2016 Aug 26;36(4):290-3. Epub 2016 Jul 26.

Service d'anatomie et cytologie pathologiques, hôpital Nord, avenue Albert-Raymond, 42055 Saint-Étienne cedex 2, France.

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http://dx.doi.org/10.1016/j.annpat.2016.03.009DOI Listing
August 2016

A 48-YEar-Old Male with a Pituitary Tumor.

Brain Pathol 2016 Jan;26(1):130-1

Department of Pathology, Hôpital Nord, Saint Etienne, CEDEX2, France.

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http://dx.doi.org/10.1111/bpa.12339DOI Listing
January 2016

Mediastinal Mature Teratoma With Malignant Carcinomatous Transformation (Somatic-Type Malignancy) With Metastatic Course.

Int J Surg Pathol 2015 Dec 24;23(8):682-4. Epub 2015 Jun 24.

University Hospital of Saint Étienne, Saint Étienne, France

We report the case of a 76-year-old patient presenting with an anterior mediastinal heterogeneous mass. Surgical biopsy revealed a solid and cystic lesion filled with hair. Pathological examination showed an atypical papillary epithelial lining without other germ cell tumor or immature teratoma associated. The final diagnosis was a mature teratoma of the mediastinum with somatic-type malignancy (carcinomatous transformation). After 8-month follow-up, subcutaneous and lymph node metastatic lesions of the carcinomatous component were identified. Subtyping of the malignant component within germ cell tumors is an important challenge for therapeutic options and prognosis.
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http://dx.doi.org/10.1177/1066896915591583DOI Listing
December 2015

Ex vivo confocal microscopy imaging to identify tumor tissue on freshly removed brain sample.

J Neurooncol 2015 Sep 2;124(2):157-64. Epub 2015 Jun 2.

Service d'Anatomie et Cytologie Pathologiques - CHU de Saint Étienne, Hôpital Nord, 42055, Saint Étienne. Cedex2, France.

Confocal microscopy is a technique able to realize "optic sections" of a tissue with increasing applications. We wondered if we could apply an ex vivo confocal microscope designed for dermatological purpose in a routine use for the most frequent brain tumors. The aim of this work was to identify tumor tissue and its histopathological hallmarks, and to assess grading criteria used in neuropathological practice without tissue loss on freshly removed brain tissue. Seven infiltrating gliomas, nine meningiomas and three metastases of carcinomas were included. We compared imaging results obtained with the confocal microscope to frozen sections, smears and tissue sections of formalin-fixed tissue. Our results show that ex vivo confocal microscopy imaging can be applied to brain tumors in order to quickly identify tumor tissue without tissue loss. It can differentiate tumors and can assess most of grading criteria. Confocal microscopy could represent a new tool to identify tumor tissue on freshly removed sample and could help in selecting areas for biobanking of tumor tissue.
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http://dx.doi.org/10.1007/s11060-015-1832-zDOI Listing
September 2015