Publications by authors named "Cui Wang"

270 Publications

Selective Separation of Am(III)/Eu(III) by the QL-DAPhen Ligand under High Acidity: Extraction, Spectroscopy, and Theoretical Calculations.

Inorg Chem 2021 Dec 3. Epub 2021 Dec 3.

Laboratory of Nuclear Energy Chemistry, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, P.R. China.

Although 1,10-phenanthroline-based ligands have recently shown vast opportunities for the separation of trivalent actinides (Ans(III)) from lanthanides (Lns(III)), the optimization and design of the extractant structure based on the phenanthroline framework remain hotspots for further improving the separation. Following the strategy of hard and soft donor atom combination, for the first time, the quinoline group was attached to the 1,10-phenanthroline skeleton, giving a lipophilic ligand, 2,9-diacyl-bis((3,4-dihydroquinoline-1((2H)-yl)-1),10-phenanthroline (QL-DAPhen)), for Am(III)/Eu(III) separation. In the presence of sodium nitrate, the ligand can effectively extract Am(III) over Eu(III) in HNO solution, with the separation factor () ranging from 29 to 44. The coordination chemistry of Eu(III) with QL-DAPhen was investigated by slope analysis, NMR titration, UV-vis titration, Fourier transform infrared spectroscopy, electrospray ionization-mass spectrometry, and theoretical calculations. The experimental results unanimously confirm that the ligand forms both 1:1 and 1:2 complexes with Eu(III), and the stability constants (log ) of each of the two complexes were obtained. Density functional theory calculations show that the Am-N bonds have more covalent characteristics than the Eu-N bonds in the complexes, which reveals the reason why the ligand preferentially bonds with Am(III). Meanwhile, the thermodynamic analysis reveals that the 1:1 complex is more thermodynamically stable than the 1:2 complex. The findings of this work have laid a solid theoretical foundation for the application of phenanthroline-based ligands in the separation of An(III) from practical systems.
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http://dx.doi.org/10.1021/acs.inorgchem.1c02916DOI Listing
December 2021

Emphysematous gastritis associated with mucormycosis in a patient with fulminant myocarditis requiring veno-arterial extracorporeal membrane oxygenation.

Rev Esp Enferm Dig 2021 12 2. Epub 2021 Dec 2.

Critical Care Medicine, The First Affiliated Hospital of Anhui Medical University.

We present the case of a 37-year-old male who admitted to our hospital with fever, weakness, limb pain for 6 days and dyspnea for 14 hours .The patient had no immune related diseases.He was rapidly diagnosed with fulminant myocarditis and progressed to severe cardiogenic shock during the early stage.Then he was treated with V-A extracorporeal membrane oxygenation (ECMO). It is worth mentioning that the patient's peripheral blood was taken for metagenomic Next-Generation Sequencing(mNGS) upon admission and the results did not find any pathogenic bacteria.But there is no further examination(such as coronary angiography and myocardial biopsy) to determine the etiology of myocarditis.
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http://dx.doi.org/10.17235/reed.2021.8477/2021DOI Listing
December 2021

Data-driven analysis and evaluation of regional agriculture for high-quality development of Anhui Province in the Yangtze River Delta.

Environ Sci Pollut Res Int 2021 Nov 17. Epub 2021 Nov 17.

Business School, Suzhou University, Suzhou, 234000, China.

This study aims to promote the high-quality development of regional agriculture. This study proposes a data-driven method for regional agricultural analysis and evaluation. Based on the data collection and processing related to regional agricultural development, the location entropy index was used to analyze the industrial agglomeration level, and the shift-share model was constructed to evaluate the industrial structure and competitiveness. Based on the above analysis and evaluation, high-quality development policy suggestions for regional agriculture were provided. Taking the agricultural development of the Yangtze River Delta from 2010 to 2019 as an example, this study shows the implementation process of the method. From the perspective of high-quality agricultural development in the Anhui Province, this paper proposes policy suggestions on industrial structure adjustment and promoting competitiveness. This study provides theoretical and methodological support for the development of high-quality regional agriculture.
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http://dx.doi.org/10.1007/s11356-021-17358-wDOI Listing
November 2021

NEP1‑40 promotes myelin regeneration via upregulation of GAP‑43 and MAP‑2 expression after focal cerebral ischemia in rats.

Mol Med Rep 2021 Dec 13;24(6). Epub 2021 Oct 13.

Department of Neurology, Dalian Medical University, Dalian, Liaoning 116033, P.R. China.

Axon regeneration after lesions to the central nervous system (CNS) is largely limited by the presence of growth inhibitory molecules expressed in myelin. Nogo‑A is a principal inhibitor of neurite outgrowth, and blocking the activity of Nogo‑A can induce axonal sprouting and functional recovery. However, there are limited data on the expression of Nogo‑A after CNS lesions, and the mechanism underlying its influences on myelin growth remains unknown. The aim of the present study was to observe the time course of Nogo‑A after cerebral ischemia/reperfusion in rats using immunohistochemistry and western blot techniques, and to test the effect of its inhibitor Nogo extracellular peptide 1‑40 (NEP1‑40) on neural plasticity proteins, growth‑associated binding protein 43 (GAP‑43) and microtubule associated protein 2 (MAP‑2), as a possible mechanism underlying myelin suppression. A classic model of middle cerebral artery occlusion (MCAO) was established in Sprague‑Dawley rats, which were divided into three groups: i) MCAO model group; ii) MCAO + saline group; and iii) MCAO + NEP1‑40 group. Rats of each group were divided into five subgroups by time points as follows: days 1, 3, 7, 14 and 28. Animals that only received sham operation were used as controls. The Nogo‑A immunoreactivity was located primarily in the cytoplasm of oligodendrocytes. The number of Nogo‑A immunoreactive cells significantly increased from day 1 to day 3 after MCAO, nearly returning to the control level at day 7, increased again at day 14 and decreased at day 28. Myelin basic protein (MBP) immunoreactivity in the ipsilateral striatum gradually decreased from day 1 to day 28 after ischemia, indicating myelin loss appeared at early time points and continuously advanced during ischemia. Then, intracerebroventricular infusion of NEP1‑40, which is a Nogo‑66 receptor antagonist peptide, was administered at days 1, 3 and 14 after MCAO. It was observed that GAP‑43 considerably increased from day 1 to day 7 and then decreased to a baseline level at day 28 compared with the control. MAP‑2 expression across days 1‑28 significantly decreased after MCAO. Administration of NEP1‑40 attenuated the reduction of MBP, and upregulated GAP‑43 and MAP‑2 expression at the corresponding time points after MCAO compared with the MCAO + saline group. The present results indicated that NEP1‑40 ameliorated myelin damage and promoted regeneration by upregulating the expression of GAP‑43 and MAP‑2 related to neuronal and axonal plasticity, which may aid with the identification of a novel molecular mechanism of restriction in CNS regeneration mediated by Nogo‑A after ischemia in rats.
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http://dx.doi.org/10.3892/mmr.2021.12484DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8524407PMC
December 2021

GIS-based for prediction and prevention of environmental geological disaster susceptibility: From a perspective of sustainable development.

Ecotoxicol Environ Saf 2021 Dec 8;226:112881. Epub 2021 Oct 8.

School of Geographical Sciences, Harbin Normal University, Harbin 150025, China; State Environmental Protection Key Laboratory of Wetland Ecology and Vegetation Restoration/School of Environment, Northeast Normal University, Jingyue Street 2555, Changchun 130017, China. Electronic address:

Geological disasters seriously threaten the safety of human life, property, ecological resources, and the environment. Effective control of geological disasters is the focus of achieving sustainable social development. The Helong City (Jilin Province, China) was selected as the case study. Combined with GIS technology, a new integrated prediction model of geological disaster susceptibility was developed to improve the accuracy of geological disaster assessment, reduce the cost of geological disaster treatment, and ensure the sustainable development of ecological environment. The research results showed that elevation and normalized difference vegetation index (NDVI) were the key factors affecting susceptibility. Compared with the conventional model, the accuracy of the developing integrated model FR-DT and FR-RF was improved by more than 6%, and the disaster points were more concentrated in the high susceptibility zone. Statistical results of disaster treatment cost estimation and gross domestic product (GDP) value showed that the integrated model can save about 10% of treatment cost, and the ratio of total GDP/disaster governance cost was higher. The performance of the integrated model FR-DT and FR-RF had obvious advantages over the conventional model in terms of prediction accuracy, prevention pertinence, and prevention cost. These research results promote the advancement of geological disaster prevention and control technology, ensure the safety of the geological environment, and are of great significance to the sustainable development of the regional economy.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112881DOI Listing
December 2021

Weighted gene co-expression network analysis unveils gene networks regulating folate biosynthesis in maize endosperm.

3 Biotech 2021 Oct 21;11(10):441. Epub 2021 Sep 21.

Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Supervision and Test Center for Ecological Environment Safety of Crops of MOA, Shanghai, China.

Folates are essential elements for human growth and development, and their deficiency can lead to serious disorders. Waxy maize is a rich source of folates; however, the regulatory mechanism underlying folate biosynthesis in the endosperm remains unclear. Here, we examined changes in the folate content of maize endosperm collected at 15, 18, 21, 24, and 27 days after pollination (DAP) using liquid chromatograph-mass spectrometry and identified genes related to folate biosynthesis using transcriptome sequencing data. The results showed that 5-methyl-tetrahydrofolate and 5,10-methylene tetrahydrofolate were the main storage forms of folates in the endosperm, and their contents were relatively high at 21-24 days. We also identified 569, 3183, 4365, and 5513 differentially expressed genes (DEGs) in different days around milk stage. Functional annotation revealed 518 transcription factors (TFs) belonging to 33 families exhibiting specific expression in at least one sampling time. The key hub genes involved in folate biosynthesis were identified by weighted gene co-expression network analysis. In total, 24,976 genes were used to construct a co-expression network with 29 co-expression modules, among which the brown and purple modules were highly related to folate biosynthesis. Further, 187 transcription factors in the brown and purple modules were considered potential transcription factors related to endosperm folate biosynthesis. These results may improve the understanding of the molecular mechanism underlying folate biosynthesis in waxy maize and lead to the development of nutritionally fortified varieties.

Supplementary Information: The online version contains supplementary material available at 10.1007/s13205-021-02974-7.
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http://dx.doi.org/10.1007/s13205-021-02974-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8455765PMC
October 2021

Hydrodynamic constraints on the energy efficiency of droplet electricity generators.

Microsyst Nanoeng 2021 21;7:49. Epub 2021 Jun 21.

Department of Mechanical Engineering, City University of Hong Kong, Hong Kong, 999077 China.

Electric energy generation from falling droplets has seen a hundred-fold rise in efficiency over the past few years. However, even these newest devices can only extract a small portion of the droplet energy. In this paper, we theoretically investigate the contributions of hydrodynamic and electric losses in limiting the efficiency of droplet electricity generators (DEG). We restrict our analysis to cases where the droplet contacts the electrode at maximum spread, which was observed to maximize the DEG efficiency. Herein, the electro-mechanical energy conversion occurs during the recoil that immediately follows droplet impact. We then identify three limits on existing droplet electric generators: (i) the impingement velocity is limited in order to maintain the droplet integrity; (ii) much of droplet mechanical energy is squandered in overcoming viscous shear force with the substrate; (iii) insufficient electrical charge of the substrate. Of all these effects, we found that up to 83% of the total energy available was lost by viscous dissipation during spreading. Minimizing this loss by using cascaded DEG devices to reduce the droplet kinetic energy may increase future devices efficiency beyond 10%.
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http://dx.doi.org/10.1038/s41378-021-00269-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8433426PMC
June 2021

Protective effect of cynaroside on sepsis-induced multiple organ injury through Nrf2/HO-1-dependent macrophage polarization.

Eur J Pharmacol 2021 Nov 22;911:174522. Epub 2021 Sep 22.

School of Life Science, Zhejiang Chinese Medical University, 310053, Hangzhou, China. Electronic address:

Cynaroside is the primary flavonoid component of honeysuckle which has been widely used as Chinese traditional medicine given its anti-inflammation properties. Overactive systemic inflammatory response and multi-organ injury are the leading causes of life-threatening sepsis. Regulation of macrophage polarization balance may act as a promising strategy for its treatment. In the present study, we aimed to investigate whether cynaroside exerted protective effects against sepsis and its potential mechanism. Building upon a sepsis mouse model, we observed cynaroside alleviated serum levels of inflammatory factors including IL-1β and TNF-α at 5 and 10 mg/kg. The pathological injury of heart, kidney and lung was remarkedly attenuated as the levels of blood urea nitrogen, creatinine, creatine kinase-MB and lactate dehydrogenase were reduced nearly 2.8-, 2.7-, 2.4-, and 2.5-fold as compared with the sepsis mice, respectively. We further demonstrated cynaroside suppressed the biomarker of pro-inflammatory macrophage M1 phenotype (iNOS+) and promotes the anti-inflammatory M2 polarization (CD206+) in the injury organs of septic mice. Mechanistic research verified cynaroside inhibited LPS-induced polarization of macrophage into M1 phenotype, which can be highly blocked by Nrf2 inhibitor. Expectedly, Nrf2 and its downstream (Heme oxygenase-1 (HO-1)) was upregulated in injury organs after treating with cynaroside, indicating the involvement of Nrf2 signaling. Taken together, the data claims cynaroside ameliorated systematic inflammation and multi-organ injury dependent on Nrf2/HO-1 pathway in septic mice.
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http://dx.doi.org/10.1016/j.ejphar.2021.174522DOI Listing
November 2021

SAP130 released by damaged tubule drives necroinflammation via miRNA-219c/Mincle signaling in acute kidney injury.

Cell Death Dis 2021 09 23;12(10):866. Epub 2021 Sep 23.

Institute of Nephrology, Zhongda Hospital, Southeast University School of Medicine, Nanjing, Jiangsu Province, 210009, China.

Tubules injury and immune cell activation are the common pathogenic mechanisms in acute kidney injury (AKI). However, the exact modes of immune cell activation following tubule damage are not fully understood. Here we uncovered that the release of cytoplasmic spliceosome associated protein 130 (SAP130) from the damaged tubular cells mediated necroinflammation by triggering macrophage activation via miRNA-219c(miR-219c)/Mincle-dependent mechanism in unilateral ureteral obstruction (UUO) and cisplatin-induced AKI mouse models, and in patients with acute tubule necrosis (ATN). In the AKI kidneys, we found that Mincle expression was tightly correlated to the necrotic tubular epithelial cells (TECs) with higher expression of SAP130, a damaged associated molecule pattern (DAMP), suggesting that SAP130 released from damaged tubular cells may trigger macrophage activation and necroinflammation. This was confirmed in vivo in which administration of SAP130-rich supernatant from dead TECs or recombinant SAP130 promoted Mincle expression and macrophage accumulation which became worsen with profound tubulointerstitial inflammation in LPS-primed Mincle WT mice but not in Mincle deficient mice. Further studies identified that Mincle was negatively regulated via miR-219c-3p in macrophages as miR-219c-3p bound Mincle 3'-UTR to inhibit Mincle translation. Besides, lentivirus-mediated renal miR-219c-3p overexpression blunted Mincle and proinflammatory cytokine expression as well as macrophage infiltration in the inflamed kidney of UUO mice. In conclusion, SAP130 is released by damaged tubules which elicit Mincle activation on macrophages and renal necroinflammation via the miR-219c-3p-dependent mechanism. Results from this study suggest that targeting miR-219c-3p/Mincle signaling may represent a novel therapy for AKI.
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http://dx.doi.org/10.1038/s41419-021-04131-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8460660PMC
September 2021

Macroscopical monolayer films of ordered arrays of gold nanoparticles as SERS substrates for quantitative detection in aqueous solutions.

Nanoscale 2021 Sep 17;13(35):14925-14934. Epub 2021 Sep 17.

State Key Laboratory of Crystal Materials, Shandong University, Jinan, 250100, P. R. China.

In this work, macroscopical monolayer films of ordered arrays of gold nanoparticles (MMF-OA-Au NPs) are successfully prepared at the interfaces of toluene-diethylene glycol (DEG) with a water volume fraction of 10% (no more than 25%), which can greatly reduce the electrostatic repulsion among NPs during the self-assembly due to the quick transfer of the remaining citrate ions into the DEG solutions containing water. Thanks to the uniformity in the intensity of SERS signals, the as-prepared MMF-OA-Au NPs transferred onto polydimethylsiloxane (PDMS) as SERS substrates (MMF-OA-Au [email protected]) can achieve quantitative detection of the analytes (such as crystal violet and malachite green) in aqueous solutions. Moreover, MMF-OA-Au [email protected] as SERS-based pH sensors can directly determine the pH value of the aqueous solution in the range of 3 to 10 by means of the established well-defined linear relationship with the intensity of the peak of COO without any calibration, instead of the intensity ratio of the Raman peaks of COO to 8a with further calculation. In addition, the as-prepared SERS-based pH sensors can still have excellent long-term durability.
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http://dx.doi.org/10.1039/d1nr03864hDOI Listing
September 2021

Study on layout optimization of sewage outfalls: a case study of wastewater treatment plants in Xiamen.

Sci Rep 2021 09 15;11(1):18326. Epub 2021 Sep 15.

Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, 361005, China.

In order to protect the offshore environment and strengthen the comprehensive rectification of sewage outfalls, an evaluation method of regional sewage outfalls by combining the marine numerical simulation and comprehensive evaluation technology was constructed, considering the marine environmental capacity and the ecological impact of sewage discharge from outfalls on the marine eco-environment sensitive areas. Then the layout rationality of each outfall was evaluated and the discharge scale was optimized with a case study of existing sewage outfalls in Xiamen. The results show that, the comprehensive evaluation score of Yundang outfall was 3 points in 2025, evaluated as the outfall with irrational layout. In 2035, the comprehensive evaluation scores of Fenglin and Dalipu outfalls were 3 and 2 points respectively, evaluated as the outfall with irrational discharge scale. It is suggested to control the scale of expansion or increase the reclaimed water reuse rate in Jimei and Gaoqi Wastewater Treatment Plants. This method has enriched the evaluation system for layout optimization of sewage outfalls, providing scientific supports for comprehensive improvement of sewage outfalls and marine environmental management.
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http://dx.doi.org/10.1038/s41598-021-97756-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8443653PMC
September 2021

Self-Photocatalysis Boosted Electrochemiluminescence Signal Amplification via In Situ Generation of the Coreactant.

Anal Chem 2021 09 31;93(36):12441-12446. Epub 2021 Aug 31.

College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao 266109, P. R. China.

The classic luminol-based electrochemiluminescence (ECL) platform generally suffers from self-decomposition of the coreactant (i.e., HO) during the reaction process, seriously hampering the luminous signal stability, as well as its practical application. To address this issue, apart from the introduction of complex exogenous species, preoxidation of the luminophore, and electrocatalysis for ECL signal amplification, we proposed a novel ECL model to realize the signal enhancement via self-photocatalytic generation of the coreactant HO. Interestingly, the luminescence of luminol was simultaneously utilized as the light source to promote the conversation of O to HO with the assistance of the photocatalyst resorcinol-formaldehyde resin, which could further improve the luminescence of luminol in turn. In comparison with the traditional case, this new ECL model not only exhibited obvious signal amplification but also efficiently boosted its stability of signal output. To sum up, an exogenous coreactant-free, highly stable ECL platform was obtained via simply integrating the photocatalyst RF and the luminol-based system. This work will not only inspire the design of a new integrated ECL system with a coreactant translator but also provide an ingenious insight for the construction of a new generation of ECL models.
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http://dx.doi.org/10.1021/acs.analchem.1c02605DOI Listing
September 2021

Numerical simulation of the impact of an integrated renovation project on the Maowei Sea hydrodynamic environment.

Sci Rep 2021 08 23;11(1):17059. Epub 2021 Aug 23.

Third Institute of Oceanography, Ministry of Natural Resource, Xiamen, 361005, China.

Integrated renovation projects are important for marine ecological environment protection. Three-dimensional hydrodynamics and water quality models are developed for the Maowei Sea to assess the hydrodynamic environment base on the MIKE3 software with high resolution meshes. The results showed that the flow velocity changed minimally after the project, decreasing by approximately 0.12 m/s in the east of the Maowei Sea area and increasing by approximately 0.01 m/s in the northeast of the Shajing Port. The decrease in tidal prism (~ 2.66 × 10 m) was attributed to land reclamation, and accounted for just 0.86% of the pre-project level. The water exchange half-life increased by approximately 1 day, implying a slightly reduced water exchange capacity. Siltation occurred mainly in the reclamation and dredging areas, amounting to back-silting of approximately 2 cm/year. Reclamation project is the main factor causing the decrease of tidal volume and weakening the hydrodynamics in Maowei Sea. Adaptive management is necessary for such a comprehensive regulation project. According to the result, we suggest that reclamation works should strictly prohibit and dredging schemes should optimize in the subsequent regulation works.
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http://dx.doi.org/10.1038/s41598-021-96441-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8382749PMC
August 2021

Urinary small extracellular vesicles derived CCL21 mRNA as biomarker linked with pathogenesis for diabetic nephropathy.

J Transl Med 2021 08 17;19(1):355. Epub 2021 Aug 17.

Institute of Nephrology, Zhongda Hospital, Southeast University School of Medicine, 87 Ding Jia Qiao Road, Nanjing, 210009, Jiangsu, China.

Background: Diabetic nephropathy (DN) is a leading cause of renal failure, whereas the effective and early diagnostic biomarkers are still lacking.

Methods: Fourteen cytokines and chemokines mRNA were detected in urinary extracellular vesicles (EVs) from the screening cohort including 4 healthy controls (HC), 4 diabetes mellitus (DM) and 4 biopsy-proven DN patients, and was validated in another 16 HC and 15 DM and 28 DN patients. Correlation analysis was performed between the candidate biomarkers and clinic parameters as well as kidney histological changes. The findings were also confirmed in DN rat model with single injection of STZ.

Results: The number of small EVs secreted in urine was increased in DN patients compared to DM patients and healthy controls, with expression of AQP1 (a marker of proximal tubules) and AQP2 (a marker of distal/collecting tubules). Small EVs derived CCL21 mRNA increased significantly in DN patients and correlated with level of proteinuria and eGFR. Interestingly, elevated CCL21 mRNA from urine small EVs was observed in DN patients with normal renal function and could discriminate early DN patients from DM more efficiently compared to eGFR and proteinuria. CCL21 also showed an accurate diagnostic ability in distinguishing incipient from overt DN. Histologically, CCL21 mRNA expression increased progressively with the deterioration of tubulointerstitial inflammation and showed the highest level in nodular sclerosis group (class III) in DN patients. Remarkable infiltration of CD3 positive T cells including both CD4 and CD8 positive T cell population were observed in DN patients with high-CCL21 expression. Besides, accumulation of CD3 positive T cells correlated with level of urinary small EVs derived CCL21 and co-localized with CCL21 in the tubulointerstitium in DN patients. Finally, the correlation of CCL21 expression in renal cortex and urinary small EVs was confirmed in STZ-induced DN rat model.

Conclusions: Urinary small EVs derived CCL21 mRNA may serve as early biomarker for identifying DN linked with pathogenesis. CCL21 mRNA mediated T cell infiltration may constitute the key mechanism of chronic inflammation in DN.
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http://dx.doi.org/10.1186/s12967-021-03030-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371892PMC
August 2021

Comprehensive Analysis of the Expression Profiles of Hepatic lncRNAs in the Mouse Model of Alcoholic Liver Disease.

Front Pharmacol 2021 29;12:709287. Epub 2021 Jul 29.

School of Public Health, Zhejiang Chinese Medical University, Hangzhou, China.

The worldwide prevalence of alcoholic liver disease (ALD) due to escalating alcohol consumption has presented an unprecedented pressure on human health. A few studies have determined long non-coding RNAs (lncRNAs) involved in the pathogenesis of liver diseases. However, the roles of lncRNAs in ALD development is still poorly understood. An ALD mouse model was established and confirmed. Expression profiles of lncRNAs were obtained by whole transcriptome sequencing. The altered lncRNAs in ALD mice were further verified by qRT-PCR. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were used to enrich the functions of these lncRNAs. In combination with miRNA and mRNA profiles, we constructed concise endogenous RNA (ceRNA) networks. The function of the most up/downregulated lnRNA was further verified and investigated in both ALD model and AML-12 cells. Totally, five downregulated lncRNAs were obtained and verified in ALD mice. The GO term and KEGG pathway analyses revealed that the identified lncRNAs were associated with alcohol-induced hepatic oxidative damage, cellular inflammation, and lipid metabolism. Combination the differentially modulated miRNAs and mRNAs with ceRNA network analysis, we constructed five ceRNA networks and obtained 30 miRNAs and 25 mRNAs that may participate in ALD. Further, we verified and investigate the function of the most downregulated lnc_1700023H06Rik. Depletion lnc_1700023H06Rik reduced genes encoding for lipid metabolism, especially mRNA Acat2 (ENSMUST00000159697) and Pgrmc2 (ENSMUST00000058578) both and . Knocking down lnc_1700023H06Rik induced triglyceride accumulation and lactate dehydrogenase leakage in AML12 cells, consisting with that in alcohol-treated cells. The five remarkably downregulated lncRNAs in ALD mouse model were identified as novel biomarkers, highlighting the key role of lncRNAs in the development of ALD. The effect of lnc_1700023H06Rik plays a pivotal role in lipid deposition and its pathological pathway in ALD needs further investigation.
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http://dx.doi.org/10.3389/fphar.2021.709287DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8358650PMC
July 2021

Study on the polystyrene plastic degradation in supercritical water/CO mixed environment and carbon fixation of polystyrene plastic in CO environment.

J Hazard Mater 2022 Jan 30;421:126763. Epub 2021 Jul 30.

State Key Laboratory of Multiphase Flow in Power Engineering, Xi'an Jiaotong University, Xi'an 710049, PR China.

Through the degradation of organic waste, the carbon can be extracted and converted into syngas with calorific value, and the CO generated can also be used after fixed. In this work, the gasification of polystyrene (PS) in supercritical water with CO was studied in the temperature range of 400 °C-700 °C and time range of 0-30 min. In addition, PS containing only carbon and hydrogen can react with CO to generate CO in CO atmosphere. Therefore, the degradation of PS plastics in CO atmosphere was also studied. The results showed that PS plastic was hardly gasified at 400 °C, and as the temperature rose, the liquid composition changed. In supercritical water, under certain feedstock conditions, reacting for 20 min, the carbon conversion efficiency of PS plastic reached 47.6% at 700 °C. Under all CO atmosphere conditions in this experiment, the highest proportion of CO consumed by PS degradation was 12.5%. Moreover, the higher the temperature, the smaller the average diameter of carbon microspheres in the solid product. The morphology of carbon microsphere was also related to the reaction time, and the main change came from the gasification of carbon microspheres and the precipitation and adhesion of carbon element in liquid product.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126763DOI Listing
January 2022

A novel compound heterozygous variant of the SLC12A3 gene in Gitelman syndrome with diabetes and the choices of the appropriate hypoglycemic drugs: a case report.

BMC Med Genomics 2021 08 4;14(1):198. Epub 2021 Aug 4.

Department of Nephrology, The Affiliated Qingdao Municipal Hospital of Qingdao University, No. 5 Donghai Middle Road, Qingdao, 266071, People's Republic of China.

Background: Gitelman syndrome (GS) is an autosomal recessive tubulopathy caused by mutations of the SLC12A3 gene. It is characterized by hypokalemic metabolic alkalosis, hypomagnesemia and hypocalciuria. It is universally known that both hypokalemia and hypomagnesemia can influence insulin secretion and insulin resistance, but the exact mechanisms require further study. We identified a novel deletion variant of the SLC12A3 gene and discussed the appropriate hypoglycemic drugs in Gitelman syndrome (GS) patients with type 2 diabetes.

Case Presentation: A 55-year-old diabetic female patient was hospitalized for evaluation because of paroxysmal general weakness and numbness of extremities for one year. We suspected that she was suffering from GS by initial estimation. Direct Sanger sequencing was used to analyze the causative gene SLC12A3 of GS. Oral glucose tolerance test (OGTT) was carried out to assess the glucose metabolism and insulin resistance status. Genetic analysis revealed that she was a compound heterozygote for a recurrent missense mutation c.179C > T and a novel deletion c.1740delC in SLC12A3, thus her diagnosis of GS was confirmed. The patient was treated with potassium chloride (3.0 g/d) and magnesium chloride (element magnesium 350 mg/d) on the basis of initial treatment of diabetes with hypoglycemic drug (Repaglinide, 3.0 mg/day). However, she developed frequent hypoglycemia after one week. OGTT showed that her glucose metabolism and insulin resistance much improved after potassium and magnesium supplemental therapy. Then we changed the hypoglycemic agent to a dipeptidyl peptidase-4 (DPP-4) inhibitor (Trajenta 5 mg/d), since then her blood glucose level remained normal during two-year of follow-up.

Conclusion: We have identified a novel deletion of the SLC12A3 gene and discussed the appropriate hypoglycemic drugs in Gitelman syndrome (GS) patients with type 2 diabetes. We suggested that attention need to be paid to blood glucose monitoring in GS patients, especially when hypokalemia and hypomagnesemia are corrected. Besides, the insufficient blood volume and serum electrolyte disturbance should also be taken into consideration in the selecting hypoglycemic drugs for GS patients.
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http://dx.doi.org/10.1186/s12920-021-01047-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8336329PMC
August 2021

[Arthroscopic treatment for the calcific tendinitis at soft tissues around hip].

Zhongguo Gu Shang 2021 Jul;34(7):659-64

Orthopaedics Department of Ningbo First Hospital, Ningbo 315010, Zhejiang, China.

Objective: To explore the feasibility and clinical effects of arthroscopic treatment for the calcific tendinitis at soft tissues around hip.

Methods: A total of 16 patients diagnosed as the calcific tendinitis at soft tissues around hip from May 2013 to July 2018 were retrospectively analyzed. All the 16 patients received arthroscopic procedures. There were 10 males and 6 females with an average age of 35 to 63 (44.50±6.67) years old and 9 left hips, 6 right hips were involved. The course of disease were 1 to 8(3.18±1.97) days. Clinical effects were evaluated with visual analogue scale(VAS), modified Harris hip scores (HHS), nonarthritic hip score (NAHS) and imaging examinations before operation, 1 day after operation and the final follow-up.

Results: All 16 patients successfully finished the arthroscopic procedures in 0.5 to 1.2 (0.75±0.21) hours. Primary healing of incision were obtained without any complications of infection, wound hematocele and neurovascular injury. All 16 patients received an average postoperative follow-up of 6 to 12 (9.6±2.3) months. Before operation, the VAS were 7.88±0.72, modified HHS were 29.25±3.23, NAHS were 27.42±3.08. The 1st day postoperative VAS were 2.19±0.66, modified HHS were 82.56± 5.64, NAHS were 82.11±2.94, all the difference were statistically significant between before and 1 day after operation (< 0.05). At the final follow-up, the VAS were 0.38±0.50, modified HHS were 94.31±2.82, NAHS were 94.84±2.85, all the scores were improved significantly compared to that before operation (<0.05). At final follow-up, there was no recurrence and the calcification disappeared in all patients, there was one patient feel sourness around the hip.

Conclusion: Arthroscopic treatment for the calcific tendinitis at soft tissues around hip is effective.It has advantages of minimal invasive, rapid pain relief, rapid hip joint function recovery and definite clinical effects.
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http://dx.doi.org/10.12200/j.issn.1003-0034.2021.07.014DOI Listing
July 2021

Strongly Red-Emissive Molecular Ruby [Cr(bpmp)] Surpasses [Ru(bpy)].

J Am Chem Soc 2021 Aug 23;143(30):11843-11855. Epub 2021 Jul 23.

Department of Chemistry, Johannes Gutenberg University of Mainz, Duesbergweg 10-14, 55128 Mainz, Germany.

Gaining chemical control over the thermodynamics and kinetics of photoexcited states is paramount to an efficient and sustainable utilization of photoactive transition metal complexes in a plethora of technologies. In contrast to energies of charge transfer states described by spatially separated orbitals, the energies of spin-flip states cannot straightforwardly be predicted as Pauli repulsion and the nephelauxetic effect play key roles. Guided by multireference quantum chemical calculations, we report a novel highly luminescent spin-flip emitter with a quantum chemically predicted blue-shifted luminescence. The spin-flip emission band of the chromium complex [Cr(bpmp)] (bpmp = 2,6-bis(2-pyridylmethyl)pyridine) shifted to higher energy from ca. 780 nm observed for known highly emissive chromium(III) complexes to 709 nm. The photoluminescence quantum yields climb to 20%, and very long excited state lifetimes in the millisecond range are achieved at room temperature in acidic DO solution. Partial ligand deuteration increases the quantum yield to 25%. The high excited state energy of [Cr(bpmp)] and its facile reduction to [Cr(bpmp)] result in a high excited state redox potential. The ligand's methylene bridge acts as a Brønsted acid quenching the luminescence at high pH. Combined with a pH-insensitive chromium(III) emitter, ratiometric optical pH sensing is achieved with single wavelength excitation. The photophysical and ground state properties (quantum yield, lifetime, redox potential, and acid/base) of this spin-flip complex incorporating an earth-abundant metal surpass those of the classical precious metal [Ru(α-diimine)] charge transfer complexes, which are commonly employed in optical sensing and photo(redox) catalysis, underlining the bright future of these molecular ruby analogues.
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http://dx.doi.org/10.1021/jacs.1c05971DOI Listing
August 2021

Probabilistic-fuzzy risk assessment and source analysis of heavy metals in soil considering uncertainty: A case study of Jinling Reservoir in China.

Ecotoxicol Environ Saf 2021 Oct 19;222:112537. Epub 2021 Jul 19.

College of Mining, Liaoning Technical University, Fuxin 123000, China.

Considering the uncertainty caused by the random error of the sample measurement, the heterogeneity of spatial and temporal distribution of pollutants, and the traditional method of selecting a single parameter for evaluation, based on fuzzy theory, Hakanson potential ecological risk index (considering heavy metal enrichment, ecotoxicity and bioavailability) and United States Environmental Protection Agency health risk assessment system, the fuzzy ecological risk and health risk assessment methods for of heavy metals in soil were established. In the soil of the Jinling Reservoir area, Cd, which has high bioavailability, had the highest average contribution rate to RI, and thus was, regarded as a priority metal for ecological risk. Sites JL9 and JL11 were the priority areas. The heavy metals did not pose a clear hazard to human health, but children had a higher health risk. Pb and As were regarded as the priority metals for health risk. Fuzzy evaluation provided the risk interval and membership degree, contained more parameter information, quantified and reduced the uncertainty of parameters, provided more comprehensive results, and compensated for the deficiency of deterministic evaluation. As the main source of heavy metals, the intensity of agricultural activities in the study area must be controlled to avoid excessive input and accumulation of heavy metals, which may damage the ecological environment and endanger human health.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112537DOI Listing
October 2021

FAM134B-Mediated ER-Phagy in Mg-Free Solution-Induced Mitochondrial Calcium Homeostasis and Cell Death in Epileptic Hippocampal Neurons.

Neurochem Res 2021 Sep 2;46(9):2485-2494. Epub 2021 Jul 2.

Department of Clinical Laboratory, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China.

Mitochondrial-associated endoplasmic reticulum (ER) membranes (MAMs) regulate calcium (Ca) homeostasis via Ca transport-related proteins such as inositol-1,4,5-triphosphate receptor (IP3R). FAM134B-mediated ER-phagy plays an important role in ER homeostasis. However, it remains unknown whether FAM134B-mediated ER-phagy affects mitochondrial Ca homeostasis and cell death through MAMs. In this study, we demonstrated that colocalization degree of FAM134B with LC3 and the LC3-II/LC3-I ratio were elevated in the hippocampal neuronal culture (HNC) model of acquired epilepsy (AE), which indicate an increased level of autophagy. In this model, FAM134B overexpression enhanced ER-phagy, while FAM134B downregulation had the opposite effect. Additionally, FAM134B overexpression significantly reversed the increases in IP3R expression and mitochondrial Ca concentration and the decrease in the ER Ca concentration in this model. FAM134B overexpression also ameliorated the AE-induced ultrastructural damage in neuronal mitochondria, decrease in mitochondrial membrane potential (mMP), cytochrome c (CytC) release and caspase-3 activation, while FAM134B downregulation induced the opposite effects. Altogether, our data indicate that FAM134B-mediated ER-phagy can attenuate AE-induced neuronal apoptosis, possibly by modulating the IP3R in MAMs to alter Ca exchange between ER and mitochondria and thus inhibit mitochondrial structural damage, a decrease in mMP, release of CytC and mitochondrial apoptosis.
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http://dx.doi.org/10.1007/s11064-021-03389-9DOI Listing
September 2021

Association between Sleep Duration and Hand Grip Strength among COPD Patients.

West J Nurs Res 2021 Jul 1:1939459211028666. Epub 2021 Jul 1.

Peking University School of Nursing, Beijing, China.

This study aimed to evaluate the association between night-time sleep duration and hand grip strength (HGS) among patients with chronic obstructive pulmonary disease (COPD). Participants aged ≥45 years were included in a nationally representative investigation clinical study in 2015. HGS was measured using dynamometers. The data on night-time sleep duration, sociodemographic information, and health-related variables were systematically collected. For analysis, sleep duration was categorized as <5 h, 5-7 h, 7 h, 7-9 h, and >9 h. Multivariable linear regression models were used to determine the possible association between the night-time sleep duration and HGS. Our results indicated that the shortest (<5 h) or the longest sleep duration (>9 h) was relevant to high risk of weaker HGS in females. In males, the shortest (<5 h) sleep duration was correlated to lower HGS. Thus, our findings clearly suggest that health care providers should focus on the potential influence of sleep duration on HGS among COPD patients.
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http://dx.doi.org/10.1177/01939459211028666DOI Listing
July 2021

Ligand-controlled and nanoconfinement-boosted luminescence employing Pt(ii) and Pd(ii) complexes: from color-tunable aggregation-enhanced dual emitters towards self-referenced oxygen reporters.

Chem Sci 2021 Jan 6;12(9):3270-3281. Epub 2021 Jan 6.

Institut für Anorganische und Analytische Chemie, Westfälische Wilhelms-Universität Münster Corrensstraße 28/30 D-48149 Münster Germany

In this work, we describe the synthesis, structural and photophysical characterization of four novel Pd(ii) and Pt(ii) complexes bearing tetradentate luminophoric ligands with high photoluminescence quantum yields ( ) and long excited state lifetimes () at room temperature, where the results were interpreted by means of DFT calculations. Incorporation of fluorine atoms into the tetradentate ligand favors aggregation and thereby, a shortened average distance between the metal centers, which provides accessibility to metal-metal-to-ligand charge-transfer (MMLCT) excimers acting as red-shifted energy traps if compared with the monomeric entities. This supramolecular approach provides an elegant way to enable room-temperature phosphorescence from Pd(ii) complexes, which are otherwise quenched by a thermal population of dissociative states due to a lower ligand field splitting. Encapsulation of these complexes in 100 nm-sized aminated polystyrene nanoparticles enables concentration-controlled aggregation-enhanced dual emission. This phenomenon facilitates the tunability of the absorption and emission colors while providing a rigidified environment supporting an enhanced up to about 80% and extended exceeding 100 μs. Additionally, these nanoarrays constitute rare examples for self-referenced oxygen reporters, since the phosphorescence of the aggregates is insensitive to external influences, whereas the monomeric species drop in luminescence lifetime and intensity with increasing triplet molecular dioxygen concentrations (diffusion-controlled quenching).
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http://dx.doi.org/10.1039/d0sc06126cDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8179353PMC
January 2021

Gastrodia remodels intestinal microflora to suppress inflammation in mice with early atherosclerosis.

Int Immunopharmacol 2021 Jul 24;96:107758. Epub 2021 May 24.

Yunnan Provincial Key Laboratory of Molecular Biology for Sinomedicine, Yunnan University of Chinese Medicine, Kunming, Yunnan, China; Key Laboratory of Microcosmic Syndrome Differentiation, Education Department of Yunnan, Kunming, Yunnan University of Chinese Medicine, Yunnan, China. Electronic address:

Atherosclsis is a critical actuator causing cardiac-cerebral vascular disease with a complicated pathogeneon, refered to the disorders of intestinal flora and persistent inflammation. Gastrodin (4-(hydroxymethyl) phenyl-β-D- Glucopyranoside) is the most abundant glucoside extracted from the Gastrodiaelata, which is a traditional Chinese herbal medicine for cardiac-cerebral vascular disease, yet its mechanisms remain little known. In the present study, the gastrodia extract and gastrodin attenuate the lipid deposition and foam cells on the inner membrane of the inner membrane of the thoracic aorta in the early atherosclerosis mice. Blood lipid detection tips that TC and LDL-C were reduced in peripheral blood after treatment with the gastrodia extract and gastrodin. Furthermore, unordered gut microbes are remodeled in terms of bacterial diversity and abundance at family and genus level. Also, the intestinal mucosa damage and permeability were reversed, accompaniedwith the reducing of inflammatory cytokines. Our findings revealed that the functions of gastrodia extract and gastrodin in cardiac-cerebral vascular disease involved to rescued gut microbes and anti-inflammation may be the mechanismof remission lipid accumulation.
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http://dx.doi.org/10.1016/j.intimp.2021.107758DOI Listing
July 2021

Multiplexed quantitative evaluation on mitochondrial toxicity of tris (2,3-dibromopropyl) phosphate in hepatocyte.

Ecotoxicol Environ Saf 2021 Sep 16;221:112425. Epub 2021 Jun 16.

Academy of Chinese Medical Science, Zhejiang Chinese Medical University, Hangzhou 310053, Zhejiang, People's Republic of China; School of Life Science, Zhejiang Chinese Medical University, Hangzhou 310053, Zhejiang, People's Republic of China. Electronic address:

The frequent detection of (2,3-dibromopropyl) phosphate (TDBPP) in environment has led to a consistent risk to organisms. However, little is known about the toxicity of TDBPP exclusive for its carcinogen. Mitochondrion that tightly relates to adverse outcomes once deteriorated is referred as a target of environmental pollutants. Here, we investigated the role of mitochondrial abnormality in development of cellular pathobiology especially lipid deposition when response to TDBPP in mitochondria-rich hepatocyte (AML12) at the same order of magnitude as the environmental concentrations (10 mol/L or below) via multiplexed quantitative high content analytic system. The present study claimed TDBPP shifted mitochondria from fusion morphology to fission phenotype charactering by less mitochondrial networks, larger mitochondrial areas and shorter branch length at 10 mol/L or above. This dynamic imbalance was triggered by high levels of fis and drp1 genes when treated with TDBPP. The deformation caused by TDBPP reciprocally influenced biogenesis through PGC1α and electron transport chains via ectopic expression of genes encoding for mitochondria complex I and III subunits. Accordingly, we observed high mitoROS level and low mitochondria membrane potential. Consequently, cells contained those abnormal mitochondria were predisposed to accumulating lipids after exposure to TDBPP. Here we showed that TDBPP deteriorated mitochondrial morphology and function, which may induce lipid generation. As for a banned while still emerged contaminant, our study also claimed further exploration on the non-carcinogenic toxicity of TDBPP.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112425DOI Listing
September 2021

Activation of AMP-Activated Protein Kinase-Sirtuin 1 Pathway Contributes to Salvianolic Acid A-Induced Browning of White Adipose Tissue in High-Fat Diet Fed Male Mice.

Front Pharmacol 2021 28;12:614406. Epub 2021 May 28.

School of Life Science, Zhejiang Chinese Medical University, Hangzhou, China.

Salvianolic acid A (Sal A), a natural polyphenolic compound extracted from (Danshen), exhibits exceptional pharmacological activities against cardiovascular diseases. While a few studies have reported anti-obesity properties of Sal A, the underlying mechanisms are largely unknown. Given the prevalence of obesity and promising potential of browning of white adipose tissue to combat obesity, recent research has focused on herbal ingredients that may promote browning and increase energy expenditure. The present study was designed to investigate the protective antiobesity mechanisms of Sal A, in part through white adipose browning. Both high-fat diet (HFD)-induced obese (DIO) male mice model and fully differentiated C3H10T1/2 adipocytes from mouse embryo fibroblasts were employed in this study. Sal A (20 and 40 mg/kg) was administrated to DIO mice by intraperitoneal injection for 13-weeks. Molecular mechanisms mediating effects of Sal A were evaluated. Sal A treatment significantly attenuated HFD-induced weight gain and lipid accumulation in epididymal fat pad. Uncoupling protein 1 (UCP-1), a specialized thermogenic protein and marker for white adipocyte browning, was significantly induced by Sal A treatment in both white adipose tissues and cultured adipocytes. Further mechanistic investigations revealed that Sal A robustly reversed HFD-decreased AMP-activated protein kinase (AMPK) phosphorylation and sirtuin 1 (SIRT1) expression in mice. Genetically silencing either AMPK or SIRT1 using siRNA abolished UCP-1 upregulation by Sal A. AMPK silencing significantly blocked Sal A-increased SIRT1 expression, while SIRT1 silencing did not affect Sal A-upregulated phosphorylated-AMPK. These findings indicate that AMPK was involved in Sal A-increased SIRT1. Sal A increases white adipose tissue browning in HFD-fed male mice and in cultured adipocytes. Thus, Sal is a potential natural therapeutic compound for treating and/or preventing obesity.
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http://dx.doi.org/10.3389/fphar.2021.614406DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193940PMC
May 2021

Emergy-based ecological efficiency evaluation and optimization method for logistics park.

Environ Sci Pollut Res Int 2021 Nov 11;28(41):58342-58354. Epub 2021 Jun 11.

College of mechanical and Electronic Engineering, Suzhou, 234000, China.

With the rapid development of logistics park, how to evaluate and optimize the ecological efficiency of logistics park to achieve its sustainable development has become a concern of academia. In order to achieve this goal, this paper puts forward a method based on emergy, which processes the data in a unified dimension. By constructing the ecological efficiency evaluation model of logistics park, it quantitatively evaluates the ecological efficiency of logistics park, and analyzes the correlation between various factors and ecological efficiency. The application results of H logistics park show that fuel oil, information technology, net profit, and waste gas are closely related to the ecological efficiency of logistics park, and the correlation coefficients are 0.8248, -0.6949, 0.8544, and 0.7661, respectively. On this basis, the paper puts forward some suggestions to improve the ecological efficiency of the logistics park. This paper provides theoretical and methodological support for the evaluation and optimization of the ecological efficiency of the logistics park.
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http://dx.doi.org/10.1007/s11356-021-14781-xDOI Listing
November 2021

Determinants of Self-Rated Health Among Middle-Aged and Elderly Patients with COPD: The China Health and Retirement Longitudinal Study.

Clin Nurs Res 2021 May 28:10547738211018818. Epub 2021 May 28.

Peking University School of Nursing, Beijing, China.

This study aimed to assess self-rated health (SRH) status and its determinants in patients with chronic obstructive pulmonary disease (COPD). Participants aged at least 45 years were included from a nationally representative investigation in 2015. The SRH assessment was divided into two groups: "good" and "not good." Independent samples -tests, chi-square tests, and Fisher's exact tests were used to determine differences in patient characteristics between the two groups. Multiple logistic models were used to determine factors influencing SRH. A total of 1,215 patients with COPD were included in the final analysis, of whom 21.2% reported favorable SRH status. The results indicated that female gender, non-smoking, and elevated peak expiratory flow correlated with better SRH, while nighttime sleep duration less than 7 hours, asthma, and disability, negatively impacted SRH. Our findings provide new evidence for the development of effective health promotion programs for the well-being of patients with COPD.
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http://dx.doi.org/10.1177/10547738211018818DOI Listing
May 2021

Transcriptome Analysis of Tetraploid and Octoploid Common Reed ().

Front Plant Sci 2021 5;12:653183. Epub 2021 May 5.

Institute of Ecology and Biodiversity, Shandong Provincial Engineering and Technology Research Center for Vegetation Ecology, School of Life Sciences, Shandong University, Qingdao, China.

Polyploidization in plants is thought to have occurred as coping mechanism with environmental stresses. Polyploidization-driven adaptation is often achieved through interplay of gene networks involved in differentially expressed genes, which triggers the plant to evolve special phenotypic traits for survival. is a cosmopolitan species with highly variable phenotypic traits and high adaptation capacity to various habitats. The species' ploidy level varies from 3x to 12x, thus it is an ideal organism to investigate the molecular evolution of polyploidy and gene regulation mediated by different numbers of chromosome copies. In this study, we used high-throughput RNAseq data as a tool, to analyze the gene expression profiles in tetraploid and octoploid . The estimated divergence time between tetraploid and octoploid was dated to the border between Pliocene and Pleistocene. This study identified 439 up- and 956 down-regulated transcripts in tetraploids compared to octoploids. Gene ontology and pathway analysis revealed that tetraploids tended to express genes responsible for reproduction and seed germination to complete the reproduction cycle early, and expressed genes related to defense against UV-B light and fungi, whereas octoploids expressed mainly genes related to thermotolerance. Most differentially expressed genes were enriched in chaperones, folding catalysts and protein processing in endoplasmic reticulum pathways. Multiple biased isoform usage of the same gene was detected in differentially expressed genes, and the ones upregulated in octoploids were related to reduced DNA methylation. Our study provides new insights into the role of polyploidization on environmental responses and potential stress tolerance in grass species.
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http://dx.doi.org/10.3389/fpls.2021.653183DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8132968PMC
May 2021
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