Publications by authors named "Cui Huang"

134 Publications

Rapid construction and enhanced vascularization of microtissue using a magnetic control method.

Biofabrication 2021 May 7;13(3). Epub 2021 May 7.

Department of Prosthodontics, Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University, School of Medicine, Shanghai 200011, People's Republic of China.

Stem cells play critical roles in tissue repair and regeneration. The construction of stem cell-derived microtissue is a promising strategy for transplanting cells into defects to improve tissue regeneration efficiency. However, rapidly constructing larger microtissues and promoting vascularization to ensure the cellular nutrient supply remain major challenges. Here, we have developed a magnetic device to rapidly construct and regulate millimeter-scale microtissues derived from magnetic nanoparticle-labeled cells. When the microtissue was cultured under a specific magnetic field, the shape of the microtissue could be changed. Importantly, cell proliferation was maintained, and angiogenesis was activated in the process of microtissue deformation. We developed a magnetic control method to treat microtissue, and the implanted microtissue showed excellent vascularization. In brief, this magnetic control technology provides a promising strategy for vascularized regenerative medicine.
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http://dx.doi.org/10.1088/1758-5090/abe4c2DOI Listing
May 2021

The Stability of Dentin Surface Biobarrier Consisting of Mesoporous Delivery System on Dentinal Tubule Occlusion and Streptococcus Mutans Biofilm Inhibition.

Int J Nanomedicine 2021 27;16:3041-3057. Epub 2021 Apr 27.

The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, People's Republic of China.

Background: The dentin exposure always leads to dentin hypersensitivity and/or caries. Given the dentin's tubular structure and low mineralization degree, reestablishing an effective biobarrier to stably protect dentin remains significantly challenging. This study reports a versatile dentin surface biobarrier consisting of a mesoporous silica-based epigallocatechin-3-gallate (EGCG)/nanohydroxyapatite delivery system and evaluates its stability on the dentinal tubule occlusion and the () biofilm inhibition.

Materials And Methods: The mesoporous delivery system was fabricated and characterized. Sensitive dentin discs were prepared and randomly allocated to three groups: 1, control group; 2, casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) group; and 3, the mesoporous delivery system group. The dentin permeability, dentinal tubule occlusion, acid and abrasion resistance, and biofilm inhibition were determined for 1 week and 1 month. The in vitro release profiles of EGCG, Ca, and P were also monitored.

Results: The mesoporous delivery system held the ability to sustainably release EGCG, Ca, and P and could persistently occlude dentinal tubules with acid and abrasion resistance, reduce the dentin permeability, and inhibit the biofilm formation for up to 1 month compared with the two other groups. The system provided prolonged stability to combat oral adverse challenges and served as an effective surface biobarrier to protect the exposed dentin.

Conclusion: The establishment of the dentin surface biobarrier consisting of a mesoporous delivery system indicates a promising strategy for the prevention and the management of dentin hypersensitivity and caries after enamel loss.
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http://dx.doi.org/10.2147/IJN.S290254DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8088303PMC
May 2021

A rapidly magnetically assembled stem cell microtissue with "hamburger" architecture and enhanced vascularization capacity.

Bioact Mater 2021 Nov 7;6(11):3756-3765. Epub 2021 Apr 7.

Department of Prosthodontics, Shanghai Engineering Research Center of Advanced Dental Technology and Materials, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China.

With the development of magnetic manipulation technology based on magnetic nanoparticles (MNPs), scaffold-free microtissues can be constructed utilizing the magnetic attraction of MNP-labeled cells. The rapid construction and vascularization of microtissues with complex hierarchical architectures are of great importance to the viability and function of stem cell microtissues. Endothelial cells are indispensable for the formation of blood vessels and can be used in the prevascularization of engineered tissue constructs. Herein, safe and rapid magnetic labeling of cells was achieved by incubation with MNPs for 1 h, and ultrathick scaffold-free microtissues with different sophisticated architectures were rapidly assembled, layer by layer, in 5 min intervals. The transplantation results showed that in a stem cell microtissue with trisection architecture, the two separated human umbilical vein endothelial cell (HUVEC) layers would spontaneously extend to the stem cell layers and connect with each other to form a spatial network of functional blood vessels, which anastomosed with the host vasculature. The "hamburger" architecture of stem cell microtissues with separated HUVEC layers could promote vascularization and stem cell survival. This study will contribute to the construction and application of structural and functional tissues or organs in the future.
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http://dx.doi.org/10.1016/j.bioactmat.2021.03.007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8044908PMC
November 2021

Experiences and attitudes of elementary school students and their parents toward online learning in China during the COVID-19 pandemic: Questionnaire Study.

J Med Internet Res 2021 Apr 16. Epub 2021 Apr 16.

Department of psychiatry, Chaohu Hospital, Anhui Medical University, 64 North Chaohu Road, Hefei, CN.

Background: Due to the widespread infection of COVID-19, an emergency homeschooling plan was rigorously implemented throughout China.

Objective: This study aimed to investigate the experiences and attitudes of elementary school students and their parents (two generations from the same family) toward online learning in China during the pandemic.

Methods: A 16-item questionnaire was distributed at the 10 day- and 40 day-mark after the first online course to 867 parent-child pairs and 141 parent-child pairs, respectively. The questionnaire comprised of questions pertaining to the course and homework's completeness, effectiveness, reliability, and abundance as well as the students' enthusiasm to take part in online classes and their satisfaction with the courses.

Results: The findings indicate that more than 91% of students exhibited high or moderate enthusiasm for participating in online classes. However, most students performed poorly in online learning classes and after school homework. Regarding satisfaction, parents' and students' average scores were 7.35 and 7.25, respectively (10-point scoring system). During the second stage of the study, parents' positive evaluations of online learning declined, including the effectiveness and reliability of the courses. Furthermore, the proportion of students who completed the courses and homework on time decreased; this difference proved statistically significant. The overall satisfaction of parents and students with online learning also declined during this second stage (7.21 vs. 7.23); however, the difference between the two stages was not statistically significant. Several of the parents (36.2%) indicated that assisting and supervising the students' online learning caused increased stress. Thirty-six percent of parents expressed dissatisfaction or suggestions concerning online learning; most parents and students hoped to return to face-to-face classes (94.9% vs. 93.5%). Finally, the results presented six main issues that parents are most concerned about: (1) disappointment regarding timely interaction in courses; (2) apprehensive about students understanding of the course; (3) increased burden of annoying adult responsibilities; (4) concern about the children's eyesight; (5) teachers' explanations were not detailed enough; (6) concerned about the decline of students' interest and attention.

Conclusions: Online learning could prevent the spread of infectious diseases while still allowing elementary school students to attain knowledge. However, children's completion of the courses and homework were not satisfactory. Furthermore, their parents often experienced stress and had many concerns and complaints. Measures such as increasing the interactivity of the courses and prohibiting teachers from assigning the task to parents could improve the effectiveness of these courses and the mental health level of parents and students.

Clinicaltrial:
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http://dx.doi.org/10.2196/24496DOI Listing
April 2021

Associations Between Childhood Trauma and the Age of First-Time Drug Use in Methamphetamine-Dependent Patients.

Front Psychiatry 2021 26;12:658205. Epub 2021 Mar 26.

Chaohu Hospital, Anhui Medical University, Hefei, China.

Childhood trauma is related to substance use disorder; however, few studies have examined the relationship between childhood trauma and the age at which the drug was first used. The aim of this study was to investigate the relationship between childhood trauma and the age of first-time drug use among methamphetamine-dependent patients. Moreover, we analyzed the characteristics of adverse family environment associated with severe childhood trauma and the risk factors for starting drugs in minors. A baseline interview was conducted with 110 participants who were in detoxification, including demographic information, past substance use, and age of first-time drug use. The participants' childhood trauma experience before 18 years of age was evaluated using the simplified version of the Childhood Trauma Questionnaire (CTQ-SF). The Chinese version of the Family Environment Scale (FES-CV) was used to assess the family environment of methamphetamine-dependent patients. Among 110 non-injecting methamphetamine-dependent patients, nearly half ( = 48, 43.6%) had moderate and severe childhood trauma. Correlation analysis showed that the age of first-time drug use negatively correlated with emotional abuse ( = -0.32, < 0.01) and physical abuse ( = -0.27, < 0.01). The age of first-time drug use negatively correlated with conflict ( = -0.20, < 0.05) and independence ( = -0.22, < 0.05) of family environment, but positively correlated with intellectual-cultural orientation ( = 0.28, < 0.01). Additionally, childhood trauma factors significantly correlated with many indexes of family environment, especially cohesion ( = -0.45, < 0.01), conflict ( = 0.49, < 0.01), and independence ( = 0.33, < 0.01). Additionally, the regression model showed that when emotional abuse increased by one point, the age of first-time drug use was 0.69 years earlier. These findings suggest that a detrimental family environment can aggravate childhood trauma, and the experience of childhood emotional or physical abuse may be an effective predictor of early drug use among methamphetamine-dependent patients.
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http://dx.doi.org/10.3389/fpsyt.2021.658205DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8044866PMC
March 2021

Use of ceramic veneers for improving esthetics and extending the service life of an existing cement-retained implant-supported ceramic restoration: A clinical report with a 3-year follow-up.

J Prosthet Dent 2021 Apr 14. Epub 2021 Apr 14.

Associate Professor and Associate Chief Physician, Department of Prosthodontics, The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, PR China. Electronic address:

This clinical report describes an approach for improving the esthetics and extending the service life of a cement-retained implant-supported ceramic single crown by using a ceramic veneer bonded to the conservatively prepared facial surface. The restoration satisfied the patient without removing and replacing the unesthetic implant-supported ceramic crown.
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http://dx.doi.org/10.1016/j.prosdent.2021.02.038DOI Listing
April 2021

The mediating role of prenatal depression in adult attachment and maternal-fetal attachment in primigravida in the third trimester.

BMC Pregnancy Childbirth 2021 Apr 16;21(1):307. Epub 2021 Apr 16.

Chaohu Hospital, Anhui Medical University, Hefei, 238000, China.

Background: Prenatal depression and adult attachment are factors that affect the establishment of an intimate relationship between a mother and fetus. The study explored differences in prenatal depression and maternal-fetal attachment (MFA) scores between different types of adult attachment and the effects of maternal depression scores and attachment dimensions on maternal intimacy with the fetus.

Methods: The Edinburgh Postnatal Depression Scale (EPDS), Experience of Close Relationship (ECR) scale, Maternal Antenatal Attachment Scale (MAAS) and a general data scale were used to investigate 260 primigravida. An exploratory analysis was performed to analyze the effects of the depression score and adult attachment on MFA.

Results: The results showed that pregnant women with insecure attachment exhibited an increased prevalence of prenatal depression, lower total MFA scores, and lower MFA quality compared with those women with secure adult attachment. The explorative analysis showed that the depression scores mediated the relationship between adult attachment avoidance and MFA quality.

Conclusions: Primigravida who had insecure adult attachment exhibited an increased prevalence of prenatal depression and lower MFA. Maternal depression and adult attachment may affect the emotional bond between a mother and fetus. This finding should be seriously considered, and timely intervention needs to take personality traits into consideration.
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http://dx.doi.org/10.1186/s12884-021-03779-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8052769PMC
April 2021

Effect of universal adhesive and silane pretreatment on bond durability of metal brackets to dental glass ceramics.

Eur J Oral Sci 2021 04;129(2):e12772

The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, China.

This study aimed to investigate the effect of universal adhesive and silane pretreatment on the bond durability of metal brackets to dental glass ceramics. Eighty lithium disilicate glass ceramic specimens were randomly assigned to one of four groups (n = 20) defined by the pretreatment and adhesive used: (i) Adper Single Bond 2; (ii) silane + Adper Single Bond 2; (iii) Single Bond Universal; and (iv) silane +Single Bond Universal. Maxillary central incisor metal brackets were bonded on the ceramic surfaces with resin composite. A shear bond strength test was conducted after 24 h of water storage and after 10,000 thermocycles. Adhesive remnant index scoring and field-emission scanning electron microscopy were performed to determine adhesives remaining on the ceramic surfaces and the ceramic ultrastructure following bracket debonding, respectively. After 10,000 thermocycles, specimens treated with Single Bond Universal preserved an appropriate bond strength between brackets and glass ceramics and showed minimum ceramic surface damage following bracket debonding, which was not the case in the other three groups. The application of a silane-containing universal adhesive without silane pretreatment achieves adequate durability of the bond of metal brackets to dental glass ceramics and allows safe debonding, which may aid in optimizing the effectiveness for orthodontic treatment.
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http://dx.doi.org/10.1111/eos.12772DOI Listing
April 2021

Optimizing glass-ceramic bonding incorporating new silane technology in an experimental universal adhesive formulation.

Dent Mater 2021 05 20;37(5):894-904. Epub 2021 Mar 20.

KU Leuven (University of Leuven), Department of Oral Health Sciences, BIOMAT & UZ Leuven (University Hospitals Leuven), Dentistry, 3000 Leuven, Belgium. Electronic address:

Objective: Incorporating silane-coupling agent into universal adhesives (UAs) to simplify adhesive luting of glass-ceramic restorations appeared ineffective due to silane's instability in an acidic aqueous solution. This study aimed to evaluate new silane technology added to an experimental UA to be bonded to glass ceramics without separate prior silanization.

Methods: Combined silane technology, consisting of 3-(aminopropyl)triethoxysilane (APTES) and γ-methacryloxypropyltriethoxysilane (γMPTES), was incorporated into an experimental UA formulation, being referred to as ADH-XTE (3M Oral Care). Immediate and aged shear bond strength (SBS) of ADH-XTE onto as-milled ('AM'), tribochemical silica-coated ('TSC'), HF-etched ('HF'), and mirror-polished ('MP') glass-ceramic CAD/CAM blocks (IPS e.max CAD) with/without separate silanization was measured (n = 10/group). The control adhesives included Scotchbond Universal ('SBU') and Scotchbond 1 XT ('SB1-XT'). The glass-ceramic surface topography and the fractography of the SBS-debonded specimens were observed by SEM.

Results: Without separate prior silanization, the experimental UA ADH-XTE, containing combined APTES/γMPTES silane technology, significantly outperformed the glass-ceramic bonding efficiency of its silane-containing SBU precursor, while it performed equally effective as SBU applied with prior silanization. Upon aging, significant reduction in SBS was recorded when ADH-XTE was bonded to TSC glass-ceramic surfaces (p < 0.05), while not to HF ones. Notably, the lowest SBS was obtained when the UAs were bonded to AM and MP glass-ceramic surfaces, in particular when applied without separate prior silanization (p < 0.05).

Significance: The glass-ceramic bonding capacity of the new combined APTES/γMPTES silane-containing UA ADH-XTE surpassed that of its SBU precursor. HF etching remains needed to durably bond to glass-ceramics.
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http://dx.doi.org/10.1016/j.dental.2021.02.021DOI Listing
May 2021

Self-esteem, social support and coping strategies of left-behind children in rural China, and the intermediary role of subjective support:a cross-sectional survey.

BMC Psychiatry 2021 03 17;21(1):158. Epub 2021 Mar 17.

Chaohu Hospital, Anhui Medical University, Hefei, 238000, China.

Background: Negative coping strategies and behavioral problems are common among Chinese left-behind children, which are relate to a variety of negative consequences. At this stage of development, the relevant factors of coping strategies need to be further studied, in which social support and self-esteem are worthy of our attention. The aim of this study is to detect the current situation of self-esteem, social support, and coping styles of left-behind children (LBC) in rural China.

Methods: 322 children from 3 schools in China enrolled in this study, including 236 LBC and 86 non-left-behind children (NLBC) to assess self-esteem, social support and coping strategies.

Results: The LBC group had lower self-esteem score and lower total social support (subjective support, objective support and support-seeking behavior) than the NLBC group. In terms of coping strategies, the LBC group was lower than the NLBC group in problem-solving and rationalization. The self-esteem score in LBC was significant positive associated with the subjective support score, objective support score, problem-solving and help-seeking score. In addition, self-esteem has significant mediating effect between subjective support and problem-solving, subjective support and help-seeking, respectively.

Conclusions: The finding indicate that Chinese LBC's self-esteem and social support need to be improved. Given the significant correlativity between self-esteem, subjective support and coping strategy, it is necessary to promote Chinese LBC's self-esteem and social support, especially subjective support.
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http://dx.doi.org/10.1186/s12888-021-03160-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7972224PMC
March 2021

Epigallocatechin-3-gallate/nanohydroxyapatite platform delivery approach to adhesive-dentin interface stability.

Mater Sci Eng C Mater Biol Appl 2021 Mar 29;122:111918. Epub 2021 Jan 29.

The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, China. Electronic address:

Current adhesive techniques allow clinicians to bond composite resin to dentin for esthetic restoration of defected tooth. However, a vulnerable adhesive-dentin interface remains clinically challenging resulting in frequent replacement of the restorations. The inappropriate management of exposed dentin plays a major role in jeopardizing the bond stability of the adhesive-dentin interface. To overcome this problem, this paper highlights an epigallocatechin-3-gallate/nanohydroxyapatite (EGCG/nHAp) platform (mesoporous silica-based) delivery approach to the adhesive-dentin interface and investigates its effectiveness on dentin bonding durability. Microtensile bond strength, interfacial nanoleakage, and in situ zymography were determined. The inhibition of Streptococcus mutans (S. mutans) biofilm formation along the adhesive-dentin interface was assessed by confocal-laser scanning microscopy, colony forming units counts, and field-emission scanning electron microscopy. Results revealed that applying the EGCG/nHAp delivery platform on exposed dentin could preserve the dentin bond strength and reduce interfacial nanoleakage after collagenase ageing; moreover, it could inactivate the activity of matrix metalloproteinase within the hybrid layer and inhibit the adhesion and biofilm formation of S. mutans. The proposed approach demonstrates great potential for stabilizing the adhesive-dentin interface to improve dentin bonding durability and prevent secondary caries progression, thereby indicating a promising strategy to prolong the service life of dental restorations.
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http://dx.doi.org/10.1016/j.msec.2021.111918DOI Listing
March 2021

Prenatal Depression in Women in the Third Trimester: Prevalence, Predictive Factors, and Relationship With Maternal-Fetal Attachment.

Front Public Health 2020 26;8:602005. Epub 2021 Jan 26.

College of Mental Health and Psychological Science, Anhui Medical University, Hefei, China.

The prevalence of prenatal depression in pregnant women has found to be high, which may adversely affect the intimacy of a mother to her fetus. Few studies have investigated the relationship between prenatal depression and maternal-fetal attachment in pregnant Chinese women. This study is thus designed to evaluate the prevalence rate, predictive factors of prenatal depression in Chinese pregnant women in the third trimester of pregnancy, and the effect of prenatal depression on maternal-fetal attachment. A total of 340 pregnant women in the third trimester of pregnancy were recruited from a hospital in Anhui Province. The Edinburgh Postpartum Depression Scale (EPDS) was rated to assess the prenatal depression; the Pittsburgh Sleep Quality Index (PSQI) and Zung Self-Rating Anxiety Scale (SAS) were used to assess sleep quality and anxiety level for all participants. The Maternal Antenatal Attachment Scale (MAAS) was used to assess maternal-fetal attachment. The prevalence of prenatal depression in the participants was high (19.1%) in our study. The scores of prenatal anxiety and sleep disorders were higher with prenatal depression than in those without prenatal depression (47.6 ± 9.5 vs. 38.9 ± 6.9; 8.3 ± 3.3 vs. 6.1 ± 2.7, all < 0.01). MAAS quality was lower in prenatal depression women than those in non-prenatal depression women (43.8 ± 5.6 vs. 46.4 ± 4.5, < 0.01). Correlation analysis showed that prenatal depression was associated with parity, prenatal education, education level, marital satisfaction, anxiety and sleep disorders (all < 0.05). Furthermore, binary logistic regression results showed that anxiety and sleep disorders were risk factors for prenatal depression. Prenatal education, higher marriage satisfaction were protective factors for prenatal depression. In addition, correlation analysis also showed that prenatal depression was positively correlated with MAAS intensity, but negatively correlated with MAAS quality. Our results indicated a high prevalence of prenatal depression in women in the third trimester. Prenatal education and higher marital satisfaction were protective factors for prenatal depression; antenatal anxiety and sleep disorders during pregnancy were risk factors for prenatal depression. Prenatal depression was negatively correlated with MAAS quality, but positively correlated with MAAS intensity.
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http://dx.doi.org/10.3389/fpubh.2020.602005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7870992PMC
May 2021

Effects of Desensitizers on Adhesive-Dentin Bond Strength: A Systematic Review and Meta-analysis.

J Adhes Dent 2021 ;23(1):7-19

Purpose: To systematically review in vitro studies that evaluated adhesive-dentin bond strength with or without the addition of desensitizers.

Materials And Methods: A search was conducted in four databases (PubMed, Embase, Web of Science, and Cochrane Library) without publication date or language limits in accordance with the Preferred Reporting Items for Systematic Reviews (PRISMA). Two reviewers selected the studies, extracted data, and assessed the risk of bias independently. The inclusion criterion was the evaluation of the effect of immediate or aged bond strength after applying desensitizer in the bonding step. The risk of bias was assessed following the method used by Sarkis-Onofre et al.41 Meta-analysis was conducted using Review Manager 5.3.

Results: A total of 1636 articles were found using the search strategy above; 59 articles were selected for full-text analysis, and 32 were systematically reviewed, with 18 considered in the meta-analysis. Results showed a significant difference among various kinds of desensitizers. The subgroup comparison showed high heterogeneity among the different kinds of desensitizers. The use of casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) and arginine-CaCO3 showed no negative effect on the immediate bond strength, whereas other desensitizers showed some influence.

Conclusion: Different desensitizers have various effects on microtensile bond strength. Desensitizers may be selected based on the bond strength and especially used to relieve tooth sensitivity after tooth preparation.
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http://dx.doi.org/10.3290/j.jad.b916811DOI Listing
February 2021

Injectable chitin hydrogels with self-healing property and biodegradability as stem cell carriers.

Carbohydr Polym 2021 Mar 30;256:117574. Epub 2020 Dec 30.

College of Chemistry and Molecular Sciences, Hubei Engineering Center of Natural Polymer-based Medical Materials, Wuhan University, Wuhan, 430072, China. Electronic address:

To meet the demands of various therapeutic tasks, injectable hydrogels with tunable mechanical properties and degradability are highly desired. Herein, we developed an injectable chitin hydrogel system with well-manipulated mechanical properties and degradability through dynamic acylhydrazone crosslinking catalyzed by 4-amino-DL-phenylalanine (Phe-NH). The mechanical properties and degradability of the hydrogels could be easily adjusted by varying the solid content, while their gelation time could be maintained at a constant level (∼130 s) by altering Phe-NH content, thereby ensuring the good injectability of hydrogels. Moreover, the chitin hydrogels showed excellent self-healing capacity with a healing efficiency up to 95 %. Owing to their superior biocompatibility and biodegradability, the chitin hydrogels could support the proliferation and multi-potent differentiations of rat bone marrow-derived stem cells, serving as a beneficial 3D scaffold for stem cell encapsulation and delivery. This work provides a promising injectable delivery vehicle of therapeutic drugs or cells for tissue regenerative medicine.
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http://dx.doi.org/10.1016/j.carbpol.2020.117574DOI Listing
March 2021

Effect of apigenin on surface-associated characteristics and adherence of Streptococcus mutans.

Dent Mater J 2020 Dec 7;39(6):933-940. Epub 2020 Oct 7.

The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University.

Apigenin is a type of flavonols that exhibits anti-caries properties. Bacterial adherence is the initial step in the forming of a stable biofilm that leads to caries. Bacterial adherence is affected by surface characteristics, including hydrophobicity and bacterial aggregation. However, the effect of apigenin on surface characteristics of cariogenic bacteria has not been reported. We aimed to examine the effects of apigenin on adherence and biofilm formation of Streptococcus mutans UA159. Hydrophobicity and bacterial aggregation, pac and gbpC gene expressions, and cytotoxicity on human dental pulp cells were also determined. Apigenin significantly inhibited the adherence and biofilm formation of S. mutans. Hydrophobicity decreased, whereas the aggregation rate was significantly increased compared with the control. Apigenin significantly suppressed pac and gbpC gene expressions. Apigenin exhibited acceptable biocompatibility on hDPCs. Thus, apigeinin may affect adherence and biofilm formation by altering the surface properties of S. mutans without obvious adverse effect on hDPCs.
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http://dx.doi.org/10.4012/dmj.2019-255DOI Listing
December 2020

Effects of resveratrol/ethanol pretreatment on dentin bonding durability.

Mater Sci Eng C Mater Biol Appl 2020 Sep 23;114:111000. Epub 2020 Apr 23.

The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, China. Electronic address:

To determine the effects of resveratrol/ethanol solution on the durability of resin-dentin bonding interfaces. Sixty-four non-caries third molars were randomly divided into four groups (n = 16) after sectioning, and then pretreated with one of the following concentrations of resveratrol/ethanol solutions: 0 (control group), 1, 10 and 20 mg/mL, followed by a universal adhesive and resin composites. All microtensile samples were divided into three subgroups: immediate group, collagenase ageing group and thermocycled group. The microtensile bond strength (MTBS), failure modes, interfacial nanoleakage and in situ zymography were measured, whereas the inhibitory effects of pretreated dentin slices on S. mutans biofilms were determined by confocal laser scanning microscopy and MTT assay. The results indicated that bonding strength was not only influenced by pretreatment factors (P < 0.05) but also ageing factors (P < 0.05). Regardless of collagenase ageing or thermocycling, the 10 mg/mL resveratrol/ethanol pretreatment group presented significantly higher (P < 0.05) MTBS and lower (P < 0.05) expression of nanoleakage than the control group, showed better inhibitory effect of matrix metalloproteinases and S. mutans activity with acceptable cytotoxicity. Meanwhile, cohesive failure in dentin decreased gradually with increasing resveratrol concentration. Therefore, the resveratrol/ethanol solution had the potential to serve as a versatile dentin primer, which can effectively improve dentin bonding durability and prevent secondary caries.
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http://dx.doi.org/10.1016/j.msec.2020.111000DOI Listing
September 2020

New perspective to improve dentin-adhesive interface stability by using dimethyl sulfoxide wet-bonding and epigallocatechin-3-gallate.

Dent Mater 2020 Sep 14. Epub 2020 Sep 14.

The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, China. Electronic address:

Objectives: To determine whether dentin-adhesive interface stability would be improved by dimethyl sulfoxide (DMSO) wet-bonding and epigallocatechin-3-gallate (EGCG).

Methods: Etched dentin surfaces from sound third molars were randomly assigned to five groups according to different pretreatments: group 1, water wet-bonding (WWB); group 2, 50% (v/v) DMSO wet-bonding (DWB); groups 3-5, 0.01, 0.1, and 1 wt% EGCG-incorporated 50% (v/v) DMSO wet-bonding (0.01%, 0.1%, and 1%EGCG/DWB). Singlebond universal adhesive was applied to the pretreated dentin surfaces, and composite buildups were constructed. Microtensile bond strength (μTBS) and interfacial nanoleakage were respectively examined after 24 h water storage or 1-month collagenase ageing. In situ zymography andStreptococcus mutans (S. mutans) biofilm formation were also investigated.

Results: After collagenase ageing, μTBS of groups 4 (0.1%EGCG/DWB) and 5 (1%EGCG/DWB) did not decrease (p > 0.05) and was higher than that of the other three groups (p < 0.05). Nanoleakage expression of groups 4 and 5 was less than that of the other three groups (p < 0.05), regardless of collagenase ageing. Metalloproteinase activities within the hybrid layer in groups 4 and 5 were suppressed. Furthermore, pretreatment with 1%EGCG/DWB (group 5) efficiently inhibited S. mutans biofilm formation along the dentin-adhesive interface.

Significance: This study suggested that the synergistic action of DMSO wet-bonding and EGCG can effectively improve dentin-adhesive interface stability. This strategy provides clinicians with promising benefits to achieve desirable dentin bonding performance and to prevent secondary caries, thereby extending the longevity of adhesive restorations.
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http://dx.doi.org/10.1016/j.dental.2020.08.009DOI Listing
September 2020

Association Between Cognitive Function and Early Life Experiences in Patients with Alcohol Use Disorder.

Front Psychiatry 2020 13;11:792. Epub 2020 Aug 13.

Department of Psychiatry, Chaohu Hospital, Anhui Medical University, Hefei, China.

Introduction: Early life experiences could be potential risk factors for the development of alcohol use disorder. In similar circumstances, it might also influence cognitive impairment in later life. However, the relationship between early life experience and cognitive function in people with alcohol use disorders is unclear. The current study examined the effects of early social environments and experiences on cognitive function in patients with alcohol use disorder.

Methods: A total of 37 adult male patients with alcohol use disorder and 30 healthy control (HC) subjects were enrolled. The MATRICS Cognitive Consensus Battery (MCCB) was used to evaluate cognitive function. The Childhood Trauma Questionnaire (CTQ) and the Revised Adult Attachment Scale (RAAS) were used to evaluate early life experiences of the participants. The RAAS was used to evaluate the attachment patterns of participants.

Results: Compared with the HC group, the alcohol use disorder group reported higher levels of childhood abuse and were more likely to form an insecure attachment style. Patients with alcohol use disorder who experienced trauma performed worse in terms of discrete cognitive parameters such as social cognition, reasoning and problem solving compared to patients without trauma. Importantly, emotional neglect and social comfort were significantly associated with individual social cognitive skills.

Conclusions: Our results suggest that the cognitive function of patients with alcohol use disorder, especially social cognitive function, is affected by early life experiences.
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http://dx.doi.org/10.3389/fpsyt.2020.00792DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7438706PMC
August 2020

Digital technologies to facilitate minimally invasive rehabilitation of a severely worn dentition: A dental technique.

J Prosthet Dent 2020 Aug 17. Epub 2020 Aug 17.

Associate chief physician, The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan, PR China; Department of Prosthodontics, School and Hospital of Stomatology, Wuhan University, Wuhan, PR China. Electronic address:

This technique report describes digital technologies to facilitate minimally invasive rehabilitation with computer-aided design and computer-aided manufacturing (CAD-CAM) veneers for a severely worn dentition. The lingual veneers without tooth preparation and occlusal veneers with minimal tooth preparation were designed from the digital scan of the interim restorations. To facilitate the cementation of multiunit lingual veneers on unprepared teeth, a custom 3D-printed guide was designed from the digital cast of the natural dentition with the lingual veneers. The guide reduced the technique sensitivity of freehand practice and allowed the verification of the complete seating of multiunit lingual veneers on unprepared teeth. The digital technique provided a valid and reliable approach to making and cementing multiple CAD-CAM veneers for the minimally invasive restoration of worn teeth.
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http://dx.doi.org/10.1016/j.prosdent.2020.05.012DOI Listing
August 2020

Coronavirus disease 2019 (COVID-19): Experiences and protocols from the Department of Prosthodontics at the Wuhan University.

J Prosthet Dent 2020 Jul 10. Epub 2020 Jul 10.

Professor and Director of Department of Prosthodontics, The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, PR China. Electronic address:

Statement Of Problem: The novel Coronavirus Disease 2019 (COVID-19) is a global pandemic, and many countries and regions are still currently in the midst of the outbreak. This pandemic has caused prosthodontics units to suspend their clinical and educational operations in academia.

Purpose: The purpose of this article was to review the experiences from the Department of Prosthodontics, Wuhan University School and Hospital of Stomatology (DP-WHUSHS), during the COVID-19 outbreak and the protocols DP-WHUSHS used to resume clinical activities after the outbreak.

Material And Methods: The descriptive approach was used in this article to provide a chronological narrative of the experiences and protocols from the DP-WHUSHS during the COVID-19 outbreak and after the outbreak.

Results: During the COVID-19 outbreak period, clinical care was provided for patients with dental emergencies by using enhanced grade 2 or grade 3 personal protective equipment (PPE). Teledentistry was used to provide care for patients with nonemergency needs. Online webinars and lectures were conducted for the predoctoral students, residents, and dentists to minimize the interruption in their education and engage the dental community amid the pandemic. Various factors were considered before clinical activities resumed after the outbreak subsided. Additional resources were allocated for facility preparation and management and employee training. New infection control and clinical operation protocols were developed to minimize the health-care-associated infection of airborne transmission diseases. The psychological health and mental wellness of the employees were emphasized. Distance or online education is still under rapid development to provide students and dentists opportunities to advance their knowledge amid the pandemic.

Conclusions: Within the limitation of this descriptive review, the following conclusions were drawn. Patient welfare and emergency needs should be considered amid the pandemic. Enhanced grade 2 or grade 3 PPE should be used during the outbreak. Multifactorial considerations for work resumption after the outbreak included facility preparation and management, training for employees, and clinical operation management. In-person psychological consultation and online mental wellness programs were available to employees to improve their mental wellness. Distance or online education was under rapid development to minimize the interruption in education for the students and to engage the dental community amid a pandemic.
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http://dx.doi.org/10.1016/j.prosdent.2020.06.004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7351407PMC
July 2020

Zinc-Calcium-Fluoride Bioglass-Based Innovative Multifunctional Dental Adhesive with Thick Adhesive Resin Film Thickness.

ACS Appl Mater Interfaces 2020 Jul 26;12(27):30120-30135. Epub 2020 Jun 26.

Department of Oral Health Sciences, BIOMAT & UZ Leuven (University Hospitals Leuven), Dentistry, KU Leuven (University of Leuven), , 3000 Leuven, Belgium.

Apart from producing high bond strength to tooth enamel and dentin, a dental adhesive with biotherapeutic potential is clinically desirable, aiming to further improve tooth restoration longevity. In this laboratory study, an experimental two-step universal adhesive, referred to as Exp_2UA, applicable in both the etch-and-rinse (E&R) and self-etch (SE) modes and combining a primer, containing 10-methacryloyloxydecyldihydrogen phosphate as a functional monomer with chemical binding potential to hydroxyapatite, with a bioglass-containing hydrophobic adhesive resin, was multifactorially investigated. In addition to primary property assessment, including measurement of bond strength, water sorption, solubility, and polymerization efficiency, the resultant adhesive-dentin interface was characterized by transmission electron microscopy (TEM), the filler composition was analyzed by energy-dispersive X-ray spectroscopy, and the bioactive potential of the adhesive was estimated by measuring the long-term ion release and assessing its antienzymatic and antibacterial potential. Four representative commercial adhesives were used as reference/controls. Application in both the E&R and SE modes resulted in a durable bonding performance to dentin, as evidenced by favorable 1 year aged bond strength data and a tight interfacial ultrastructure that, as examined by TEM, remained ultramorphologically unaltered upon 1 year of water storage aging. TEM revealed a 20 μm thick hydrophobic adhesive layer with a homogeneous bioglass filler distribution. Adequate polymerization conversion resulted in extremely low water sorption and solubility. In situ zymography revealed reduced endogenous proteolytic activity, while biofilm formation was inhibited. In conclusion, the three-/two-step E&R/SE Exp_2UA combines the high bonding potential and bond degradation resistance with long-term ion release, rendering the adhesive antienzymatic and antibacterial potential.
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http://dx.doi.org/10.1021/acsami.0c06865DOI Listing
July 2020

Yes-associated protein promotes tumour necrosis factor α-treated cementoblast mineralization partly by inactivating NF-κB pathway.

J Cell Mol Med 2020 07 8;24(14):7939-7948. Epub 2020 Jun 8.

The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, China.

Cementum regeneration, as one of the most difficult challenges of periodontal regeneration, is influenced by inflammatory factors. Inflammation may hamper or promote periodontal tissue repair under different circumstances, as it is found to do in dentin-pulp complex and bone tissue. Our team demonstrated that YAP promotes mineralization of OCCM, a cementoblast cell line. However, the effect of YAP on its mineralization under inflammatory microenvironment is unclear. In this study, cementogenesis in vitro was up-regulated after transient TNF-α treatment for 30 minutes. YAP expression also was increased by TNF-α treatment. YAP overexpression promoted OCCM mineralization after the cells were transiently treated with TNF-α because YAP overexpression inhibited NF-κB pathway activity, while YAP knockdown elevated it. The inhibited mineralization potential and activated NF-κB pathway activity by YAP knockdown also were partly rescued by the application of the NF-κB inhibitor Bay 11-7082. These results demonstrated that YAP plays a positive role in the mineralization of TNF-α transiently treated cementoblast, partly by inhibiting the NF-κB pathway activity.
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http://dx.doi.org/10.1111/jcmm.15426DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7348144PMC
July 2020

Structural/Chemical Characterization and Bond Strength of a New Self-Adhesive Bulk-fill Restorative.

J Adhes Dent 2020 ;22(1):85-97

Purpose: The material structure and chemical elemental composition of a new self-adhesive composite hybrid were investigated. The bonding performance when applied on flat (FLAT) vs high C-factor class-I cavity-bottom (CAVITY) dentin and in light-cure (LC) vs self-cure (SC) mode was determined.

Materials And Methods: The self-adhesive bulk-fill composite Surefil One (Su-O; Dentsply Sirona) was compared with the resin-modified glass-ionomer Fuji II LC Improved (Fuji2LC; GC) and the ion-releasing alkasite material Cention N (CentionN; Ivoclar Vivadent). The material structure was examined with SEM and TEM, while the chemical elemental composition was analyzed using EDS. The immediate and aged microtensile bond strength (μTBS) of Su-O_LC/SC was compared to that of Fuji2LC applied without any pre-treatment, and to that of CentionN applied following bonding with Adhese Universal (AU) (Ivoclar Vivadent) in self-etch mode (AU/CentionN). All restorative materials were bonded onto FLAT and CAVITY dentin. Statistical analysis was performed with the Kruskal-Wallis nonparametric test.

Results: EDS analysis revealed that Su-O was richer in C and P than the reference restorative materials. Applied to FLAT dentin, the significantly highest immediate and aged μTBS were recorded for AU/CentionN, which were not significantly different only from Su-O_LC. Applied to CAVITY dentin, the significantly highest immediate μTBS was recorded for AU/CentionN, which did not differ significantly only from Su-O_SC. Su-O_LC bonded to CAVITY dentin suffered from a high incidence of pre-test failures.

Conclusion: While Su-O_LC bonded effectively and durably to FLAT dentin, Su-O_SC bonded more favorably than Su-O_LC in class-I cavities, which was probably related to shrinkage stress variously challenging the respective bond.
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http://dx.doi.org/10.3290/j.jad.a44000DOI Listing
February 2020

Bonding Efficacy of a New Self-Adhesive Restorative onto Flat Dentin vs Class-I Cavity-bottom Dentin.

J Adhes Dent 2020 ;22(1):65-77

Purpose: This study investigated the bonding efficacy of a new so-called self-adhesive composite hybrid onto flat (FLAT) and high C-factor class-I cavity-bottom (CAVITY) dentin.

Materials And Methods: The immediate and aged (50,000 thermocycles) microtensile bond strength (μTBS) to FLAT and CAVITY dentin of the experimental self-adhesive bulk-fill restorative (K-0180 ASAR pilot [ASAR-pilot], Dentsply Sirona) was compared to that of two universal adhesives applied in self-etch mode and combined with a bulk-fill composite (Prime&Bond Elect/QuiXfil [P&Be/QuiXF], Prime&Bond Active/QuiXfil [P&Ba/QuiXF], both Dentsply Sirona), two pre-conditioned materials (Activa Bioactive-Restorative [Activa], Pulpdent; Fuji II LC Improved [Fuji2LC], GC); and one bulk-fill glass-hybrid restorative (Equia Forte Fil [EquiaF], GC). Statistically significant differences were recorded using Welch's ANOVA with Games-Howell contrast (p < 0.05).

Results: No significant difference in immediate μTBS was recorded when the restorative materials were applied onto FLAT dentin, except for Activa_FLAT and EquiaF_FLAT. When bonded to CAVITY dentin, the significantly highest μTBS was recorded for Fuji2LC_CAVITY (layer filled), and was not significantly different only from P&Ba/QuiXF_CAVITY. Upon aging, the highest μTBS to flat dentin was achieved by ASAR-pilot_FLAT, which was not significantly different from P&Be/QuiXF_FLAT and Fuji2LC_FLAT. No significant difference between immediate and aged μTBS was recorded for ASAR-pilot when bonded onto FLAT or CAVITY dentin; the latter, however, was associated with low bond strength.

Conclusion: Favorable bonding performance was found for the new self-adhesive bulk-fill composite hybrid ASAR-pilot when bonded to flat dentin. However, much lower bond strength was recorded when ASAR-pilot was bonded to high C-factor cavity-bottom dentin.
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http://dx.doi.org/10.3290/j.jad.a43999DOI Listing
February 2020

Comparative cytotoxicity of halogenated aromatic DBPs and implications of the corresponding developed QSAR model to toxicity mechanisms of those DBPs: Binding interactions between aromatic DBPs and catalase play an important role.

Water Res 2020 Mar 8;170:115283. Epub 2019 Nov 8.

State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China.

Halogenated aromatic disinfection byproducts (DBPs) are a new group of emerging DBPs identified recently. They have been detected in disinfected drinking water, wastewater effluents, recreational water and oil/gas produced water, at concentrations of ng/L to μg/L in general. Previously studies have demonstrated that most of them can induce developmental toxicity and growth inhibition in aquatic organisms based on in vivo bioassays. In this study, to further understand the adverse effects of aromatic DBPs to human health, the comparative cytotoxicity of 15 halogenated aromatic DBPs belonging to four subgroups (i.e., halophenols, halonitrophenols, halohydroxybenzaldehydes and halohydroxybenzoic acids) was evaluated with mammalian Chinese Hamster Ovary cells. The results indicated that the selected aromatic DBPs exhibited an in vitro toxicity rank order of halonitrophenols > halophenols > halohydroxybenzaldehydes > halohydroxybenzoic acids. The potential toxicity mechanisms involved with the antioxidant system were investigated by using molecular docking analysis between key antioxidant enzymes (i.e., catalase, superoxide dismutase, and glutathione S-transferase) and aromatic DBPs. Based on the observed cytotoxicity data and screening the candidate descriptors (including binding energies between the aromatic DBPs and key antioxidant enzymes as well as physical-chemical/quantum-chemical/topological descriptors), a QSAR model was developed as log (LC)  = - 1.050E + 0.300E - 0.238E- 0.164, indicating the importance of the interactions of aromatic DBPs towards catalase and the electrophilic/nucleophilic reactivity of aromatic DBPs in the toxicity mechanisms. In addition, the occurrence of the aromatic DBPs in tap water and finished water was studied in a mega city Shenzhen located in South China. Results showed that halogenated aromatic DBPs commonly existed in Shenzhen drinking water at ng/L levels, and three nitrogenous aromatic DBPs were detected in real drinking water for the first time. The major toxicity drivers among the target aromatic DBPs were identified through the integration of the measured concentrations and observed cytotoxicity; notably, DBPs with the highest concentrations may not contribute the highest proportions of overall toxicity.
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http://dx.doi.org/10.1016/j.watres.2019.115283DOI Listing
March 2020

Intra/Extracellular Lactic Acid Exhaustion for Synergistic Metabolic Therapy and Immunotherapy of Tumors.

Adv Mater 2019 Dec 6;31(51):e1904639. Epub 2019 Nov 6.

Key Laboratory of Biomedical Polymers of Ministry of Education and Department of Chemistry, Wuhan University, Wuhan, 430072, P. R. China.

Regulating the tumor microenvironment (TME) has been a promising strategy to improve antitumor therapy. Here, a red blood cell membrane (mRBC)-camouflaged hollow MnO (HMnO ) catalytic nanosystem embedded with lactate oxidase (LOX) and a glycolysis inhibitor (denoted as PMLR) is constructed for intra/extracellular lactic acid exhaustion as well as synergistic metabolic therapy and immunotherapy of tumor. Benefiting from the long-circulation property of the mRBC, the nanosystem can gradually accumulate in a tumor site through the enhanced permeability and retention (EPR) effect. The extracellular nanosystem consumes lactic acid in the TME by catalyzing its oxidation reaction via LOX. Meanwhile, the intracellular nanosystem releases the glycolysis inhibitor to cut off the source of lactic acid, as well as achieve antitumor metabolic therapy through the blockade of the adenosine triphosphate (ATP) supply. Both the extracellular and intracellular processes can be sensitized by O , which can be produced during the decomposition of endogenous H O catalyzed by the PMLR nanosystem. The results show that the PMLR nanosystem can ceaselessly remove lactic acid, and then lead to an immunocompetent TME. Moreover, this TME regulation strategy can effectively improve the antitumor effect of anti-PDL1 therapy without the employment of any immune agonists to avoid the autoimmunity.
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http://dx.doi.org/10.1002/adma.201904639DOI Listing
December 2019

Wnt1 silencing enhances neurotoxicity induced by paraquat and maneb in SH-SY5Y cells.

Exp Ther Med 2019 Nov 30;18(5):3643-3649. Epub 2019 Aug 30.

Department of Toxicology, School of Public Health, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China.

Wingless (Wnt) signaling regulates the proliferation and differentiation of midbrain dopamine (DA) neurons. Paraquat (PQ) and maneb (MB) are environmental pollutants that can be used to model Parkinson's disease (PD) in rodents. A previous study demonstrated that developmental exposure to PQ and MB affects the expression of Wnt1, Wnt5a, nuclear receptor-related factor 1 (NURR1) and tyrosine hydroxylase (TH). However, how Wnt signaling regulates these developmental factors is yet to be determined. To explore this, SH-SY5Y cells were exposed to PQ and MB. The results of the current study indicated that exposure to PQ and MB decreased Wnt1, β-catenin, NURR1 and TH levels and increased Wnt5a levels. Furthermore, Wnt1 silencing has the same effect as exposure to PQ and MB. Additionally, the neurotoxicity induced by PQ and MB is more severe in siWnt1-SH-SY5Y cells compared with normal SH-SY5Y cells. Therefore, Wnt1 may serve an important role in regulating developmental DA factors, and may be a candidate gene for PD diagnosis or gene therapy.
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http://dx.doi.org/10.3892/etm.2019.7963DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6777272PMC
November 2019

Combination of baicalein and ethanol-wet-bonding improves dentin bonding durability.

J Dent 2019 11 3;90:103207. Epub 2019 Oct 3.

The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedical Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, China. Electronic address:

Objectives: This study aimed to investigate the potential of baicalein combined with ethanol-wet bonding (EWB) in improving dentin bonding durability.

Methods: Sixty caries-free human third molars were randomly allocated into four groups and pretreated with solutions after sectioning and polishing. The pretreatments were prepared via dissolving baicalein in ethanol at concentrations of 0, 0.01%, 0.05% and 0.1% (w/v). Microtensile bond strength (MTBS) test, failure mode analysis and interfacial nanoleakage evaluation were conducted immediately or after thermocycling or 1 month of collagenase aging. In situ zymography, contact angle, antibacterial activity and bioactivity were comprehensively assessed.

Results: Results demonstrated that the three experimental groups exhibited higher MTBS and lower nanoleakage expression regardless of aging. MMP activity within hybrid layer and Streptococcus. mutans biofilm formation were inhibited in the experimental groups in a dose-dependent manner. Baicalein also reduced reactive oxygen species (ROS) expression in human dental pulp cells and resisted adhesive-induced cytotoxicity. Baicalein exhibited remarkable capabilities at concentrations higher than 0.05% (w/v).

Conclusion: Baicalein is a prospective candidate as bioactive dentin bonding agent. Combined with EWB, baicalein may form a functional bonding interface, thereby enhancing dentin bond strength and durability.

Significance: Joint efforts by baicalein and EWB provides a novel therapeutic strategy for obtaining ideal adhesive-dentin interface and prolonging the longevity of restorations.
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http://dx.doi.org/10.1016/j.jdent.2019.103207DOI Listing
November 2019

Bonding to enamel using alternative Enamel Conditioner/etchants.

Dent Mater 2019 10 14;35(10):1415-1429. Epub 2019 Aug 14.

KU Leuven (University of Leuven), Department of Oral Health Sciences, BIOMAT & UZ Leuven (University Hospitals Leuven), Dentistry, Leuven 3000, Belgium. Electronic address:

Objective: Enamel bond durability of three new alternative etchants combined with three representative adhesives was determined.

Methods: The 'immediate' and 'aged' micro-tensile bond strength (μTBS) of the 3-step etch&rinse adhesive OptiBond FL ('O-FL', Kerr), 2-step self-etch (SE) adhesive Clearfil SE Bond 2 ('C-SE2', Kuraray Noritake) and universal adhesive Adhese Universal ('ADU', Ivoclar Vivadent) were measured when bonded to enamel following either a proprietary organic acid-containing Enamel Conditioner ('EC', Shofu), a phosphoric-acid monomer-containing Multi Etchant ('ME', Yamakin: 10-methacryloyloxy tetraethylene glycol dihydrogenphosphate or MTEGP), or a metal salt-based ZON etchant ('ZON', Ivoclar Vivadent: ZrO(NO)). All alternative etchants were used in replacement of phosphoric acid, the latter (K-Etchant, Kuraray Noritake) also used with O-FL and ADU, in addition to C-SE2 that was solely used in SE mode (controls). The enamel-etching patterns and de-bonded fracture surfaces were examined by SEM, while the interfaces with enamel were ultra-morphologically characterized by TEM.

Results: No statistically significant difference in immediate and aged μTBS, obtained by combining the three alternative etchants with the three adhesives, was recorded as compared with the respective controls, except for ME combined with O-FL. Upon aging, significant reduction in μTBS was recorded for the ME/C-SE2 and ME/ADU combinations. The percentage of adhesive failures increased with aging. SEM revealed similar etching patterns produced by EC and ZON as by classic phosphoric-acid etching, for which also numerous micro-resin tags at the adhesive-enamel interface were disclosed.

Significance: Durable bonding to enamel was generally obtained for all etchant/adhesive combinations with the exception of the ME/O-FL combination.
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http://dx.doi.org/10.1016/j.dental.2019.07.022DOI Listing
October 2019

Polymer conjugation optimizes EDTA as a calcium-chelating agent that exclusively removes extrafibrillar minerals from mineralized collagen.

Acta Biomater 2019 05 3;90:424-440. Epub 2019 Apr 3.

College of Dental Medicine, The Dental College of Georgia, Augusta, GA, USA. Electronic address:

During development of mineralized collagenous tissues, intrafibrillar mineralization is achieved by preventing mineralization precursor inhibitors that are larger than 40 kDa from entering the collagen fibrils. Such a property is incorporated in the design of a calcium chelator for dentin bonding in the etch-and-rinse technique that selectively demineralizes extrafibrillar apatite while leaving the intrafibrillar minerals intact. This strategy prevents complete demineralization of collagen fibrils, avoids collapse of collagen that blocks resin infiltration after air-drying, and protects the completely demineralized fibrils from bacteria colonization and degradation by endogenous proteases after resin bonding. In the present study, a water-soluble glycol chitosan-EDTA (GCE) conditioner was synthesized by conjugation of EDTA, an effective calcium chelator, to high molecular weight glycol chitosan, which exhibits weak chelation property. The GCE conjugate was purified, characterized by FTIR, H NMR, isothermal titration calorimetry and ICP-AES, and subjected to size exclusion dialysis to recover molecules that are >40 kDa. The optimal concentration and application time for etching dentin were determined by bond strength testing to ensure that the dentin bonding results were comparable to phosphoric acid etching, and maintained equivalent bond strength after air-drying of the conditioned collagen matrix. Extrafibrillar demineralization was validated with transmission electron microscopy. Inhibition of endogenous dentin proteases was confirmed using in-situ zymography. The water-soluble GCE dentin conditioner was non-cytotoxic and possessed antibacterial activities against planktonic and single-species biofilms, supporting its ongoing development as a dentin conditioner with air-drying, anti-proteolytic and antibacterial properties to enhance the durability of bonds created using the etch-and-rinse bonding technique. STATEMENT OF SIGNIFICANCE: The current state-of-the-art techniques for filling decayed teeth with plastic tooth-colored materials require conditioning the mineralized, biofilm-covered, decayed dentin with acids or acid resin monomers to create a surface layer of completely- or partially-demineralized collagen matrix for the infiltration of adhesive resin monomers. Nevertheless, fillings prepared using these strategies are not as durable as consumers have anticipated. Conjugation of polymeric glycol chitosan with EDTA produces a new conditioner for dentin bonding that demineralizes only extrafibrillar dentin, reduces endogenous protease activities and kills biofilm bacteria. The high molecular weight glycol chitosan-EDTA is non-cytotoxic to the key regenerative players within the dentin-pulp complex. This advance permits dry bonding and the use of hydrophobic resins.
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http://dx.doi.org/10.1016/j.actbio.2019.04.011DOI Listing
May 2019