Publications by authors named "Cui Li"

970 Publications

Further Characterization of Glycoform-Selective Prions of Variably Protease-Sensitive Prionopathy.

Pathogens 2021 Apr 23;10(5). Epub 2021 Apr 23.

Department of Pathology, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA.

Prion is an infectious protein (PrP) that is derived from a cellular glycoprotein (PrP) through a conformational transition and associated with a group of prion diseases in animals and humans. Characterization of proteinase K (PK)-resistant PrP by western blotting has been critical to diagnosis and understanding of prion diseases including Creutzfeldt-Jakob disease (CJD) and Gerstmann-Sträussler-Scheinker (GSS) disease in humans. However, formation as well as biochemical and biological properties of the glycoform-selective PrP in variably protease-sensitive prionopathy (VPSPr) remain poorly understood. Here we reveal that formation of the ladder-like PrP in VPSPr is a PK-dependent two-step process, which is enhanced by basic pH. Two sets of PrP fragments can be identified with antibodies directed against an intermediate or a C-terminal domain of the protein. Moreover, antibodies directed against specific PrP glycoforms reveal faster electrophoretic migrations of PrP fragments mono-glycosylated at residue 181 and 197 in VPSPr than those in sporadic CJD (sCJD). Finally, RT-QuIC assay indicates that PrP-seeding activity is lower and its lag time is longer in VPSPr than in sCJD. Our results suggest that the glycoform-selective PrP in VPSPr is associated with altered glycosylation, resulting in different PK-truncation and aggregation seeding activity compared to PrP in sCJD.
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http://dx.doi.org/10.3390/pathogens10050513DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8146342PMC
April 2021

Juglone regulates gut microbiota and Th17/Treg balance in DSS-induced ulcerative colitis.

Int Immunopharmacol 2021 Apr 26;97:107683. Epub 2021 Apr 26.

Affliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, Jiangsu, PR China. Electronic address:

Juglone, mainly isolates from the green walnut husks of Juglans mandshurica, exhibits anti-cancer and anti-inflammaroty activities. But its protection on ulcerative colitis (UC) has never been explored. In this study, we first evaluated whether juglone ameliorated UC, and investigated its effects on gut microbiota and Th17/Treg balance in DSS-induced UC mice model. The model was established by administrating 2.7% DSS for seven days. Juglone was given daily by gavage for ten days, once a day. The disease activity index (DAI) decrease and pathological characteristics improvement demonstrated that the UC in mice was alleviated by juglone. Juglone treatment significantly inhibited the protein levels of IL-6, TNF-α and IL-1β, improved the protein expression of IL-10. In addition, juglone altered microbial diversity and gut microbiota composition, including the enhancement of the ratio of Firmicutes to Bacteroidota and the abundance of Actinobacteriota, and decrease of the abundance of Verrucomicrobiota. Juglone treatment also inhibited the protein expressions of IL-6, STAT3 and RORγt, meanwhile improved the protein level of FOXP3. Furthermore, juglone inhibited Th17 development and increased Treg generation, beneficial to Th17/Treg balance. Together, we herein provided the first evidence to support that juglone, especially the high dose, possibly protected mice against UC by modulating gut microbiota and restoring Th17/Treg homeostasis.
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http://dx.doi.org/10.1016/j.intimp.2021.107683DOI Listing
April 2021

Separation and anti-inflammatory evaluation of phytochemical constituents from Pleurospermum candollei (Apiaceae) by high-speed countercurrent chromatography with continuous sample load.

J Sep Sci 2021 Jul 22;44(13):2663-2673. Epub 2021 May 22.

School of Pharmaceutical Sciences and Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu Universit of Technology (Shandong Academy of Sciences), Jinan, P. R. China.

Pleurospermum (Apiaceae) species possess a wide range of biological properties viz. analgesic, anti-inflammatory, antimalarial, and so on. Pleurospermum candollei (DC.) Benth. Ex C. B. Clark. is reported to cure diarrhea, gastric, respiratory, stomach, abdominal, joint, and back pain problems. In addition, it is also used for both male and female infertility. The present study deals with an efficient technique using high-speed countercurrent chromatography for separation of chemical components from the methanol extract of P. candollei. Notably, nine main compounds namely luteolin 7-O-glucoside (1), oxypeucedanin hydrate (2), pabulenol (3), bergapten (4), heptadecanoic acid (5), (E)-isoelemicin (6), trans-asarone (7), α-linolenic acid (8), and isoimperatorin (9) were very efficiently separated and isolated in pure form. Multiple injections were applied followed by two off-line recycling high-speed countercurrent chromatography. The inhibitory effect of nitric oxide production of all compounds was tested in the presence of 200 ng/mL lipopolysaccharide in RAW264.7 mice macrophage cells. The results demonstrated that compounds 7 and 8 effectively inhibited nitric oxide production, with IC values of 28.44 and 53.18 μM, respectively. This study thus validates the traditional claim of using P. candollei. Taken together, these findings will be useful in future research to find a potential candidate with anti-inflammatory properties.
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http://dx.doi.org/10.1002/jssc.202100155DOI Listing
July 2021

RHPN1‑AS1 promotes ovarian carcinogenesis by sponging miR‑485‑5p and releasing TPX2 mRNA.

Authors:
Shoubin Cui Cui Li

Oncol Rep 2021 06 28;45(6). Epub 2021 Apr 28.

Department of Gynaecology and Obstetrics, Yantai Affiliated Hospital of Binzhou Medical University, Yantai, Shandong 264100, P.R. China.

Long non‑coding RNAs (lncRNAs) play a crucial role in cancer development. However, researchers have yet to identify the underlying association between lncRNAs and ovarian cancer (OC). The aim of the present study was to examine the effect of lncRNA RHPN1‑AS1 (RHPN1‑AS1) on OC cells and tissues. Reverse transcriptase‑quantitative PCR (RT‑qPCR) was utilized to quantify RHPN1‑AS1, miR‑485‑5p, and mRNA expression in samples with OC. Luciferase‑reporter assay, RNA immunoprecipitation (RIP) assay, and RNA pull‑down assay were then employed to validate the target relationship among RHPN1‑AS1, miR‑485‑5p and . Cell Counting Kit‑8, BrdU, wound‑healing, cell‑adhesion, and flow cytometry assays were also employed to assess cell viability, proliferation, migration, adhesion and apoptosis, respectively, in SKOV3 and OVCAR3 cell lines. Findings revealed that RHPN1‑AS1 demonstrated a higher expression level in OC cell lines and tissues. In addition, RHPN1‑AS1 enhanced the adhesion, proliferation and migration of OC cell lines but decreased apoptosis of OC cells. It was also observed that the relationship between RHPN1‑AS1 and miR‑485‑5p was negative and that RHPN1‑AS1 could sponge miR‑485‑5p to regulate the proliferation, apoptosis, adhesion, and migration abilities of OC cells. Moreover, was targeted by miR‑485‑5p and was significantly overexpressed in OC cell lines and tissues. Experimental investigations also revealed that promoted the proliferation, adhesion, and migration of OC cells but suppressed the apoptosis of SKOV3 and OVCAR3 cells. In summary, RHPN1‑AS1 played a tumor promotive role by sponging miR‑485‑5p to increase expression in OC tumorigenesis.
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http://dx.doi.org/10.3892/or.2021.8062DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8082340PMC
June 2021

Argonaute integrated single-tube PCR system enables supersensitive detection of rare mutations.

Nucleic Acids Res 2021 07;49(13):e75

State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.

Technological advances in rare DNA mutations detection have revolutionized the diagnosis and monitoring of tumors, but they are still limited by the lack of supersensitive and high-coverage procedures for identifying low-abundance mutations. Here, we describe a single-tube, multiplex PCR-based system, A-Star, that involves a hyperthermophilic Argonaute from Pyrococcus furiosus (PfAgo) for highly efficient detection of rare mutations beneficial from its compatibility with DNA polymerase. This novel technique uses a specific guide design strategy to allow PfAgo selective cleavage with single-nucleotide resolution at 94°C, thus mostly eliminating wild-type DNA in the denaturation step and efficiently amplifying rare mutant DNA during the PCR process. The integrated single-tube system achieved great efficiency for enriching rare mutations compared with a divided system separating the cleavage and amplification. Thus, A-Star enables easy detection and quantification of 0.01% rare mutations with ≥5500-fold increase in efficiency. The feasibility of A-Star was also demonstrated for detecting oncogenic mutations in solid tumor tissues and blood samples. Remarkably, A-Star achieved simultaneous detection of multiple oncogenes through a simple single-tube reaction by orthogonal guide-directed specific cleavage. This study demonstrates a supersensitive and rapid nucleic acid detection system with promising potential for both research and therapeutic applications.
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http://dx.doi.org/10.1093/nar/gkab274DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8287959PMC
July 2021

Overexpression of ATP-binding cassette transporters associated with sulfoxaflor resistance in Aphis gossypii glover.

Pest Manag Sci 2021 Apr 26. Epub 2021 Apr 26.

Key Laboratory of Integrated Pest Management in Crops, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Ministry of Agriculture and Rural Affairs, Beijing, China.

Background: Sulfoxaflor is a new insecticide for controlling against Aphis gossypii in the field. ATP-binding cassette (ABC) transporters belong to a large superfamily of proteins and play an important role in the detoxification process. However, the potential role of ABC transporters in sulfoxaflor resistance in A. gossypii is unknown.

Results: In this study, an ABC transporter inhibitor, verapamil, dramatically increased the toxicity of sulfoxaflor in the resistant population with a synergistic ratio of 8.55. However, verapamil did not synergize sulfoxaflor toxicity in the susceptible population. The contents of ABC transporters were significantly increased in the Sul-R population. Based on RT-qPCR analysis, 10 of 23 ABC transcripts, ABCA1, ABCA2, ABCB1, ABCB5, ABCD1, ABCG7, ABCG16, ABCG26, ABCG27, and MRP7, were up-regulated in the Sul-R population compared to the Sus population. Meanwhile, inductive effects of ABCA1, ABCD1, ABCG7 and ABCG26 by sulfoxaflor were found in A. gossypii. Furthermore, knockdown of ABCA1 and ABCD1 using RNAi significantly increased the sulfoxaflor sensitivity in Sul-R aphids.

Conclusion: These results suggested that ABC transporters, especially the ABCA1 and ABCD1 genes, might be related with sulfoxaflor resistance in A. gossypii. This study will promote further work to validate the functional roles of these ABCs in sulfoxaflor resistance and might be helpful for the management of sulfoxaflor-resistant A. gossypii.
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http://dx.doi.org/10.1002/ps.6431DOI Listing
April 2021

Overexpression of Multiple UDP-Glycosyltransferase Genes Involved in Sulfoxaflor Resistance in Glover.

J Agric Food Chem 2021 May 20;69(17):5198-5205. Epub 2021 Apr 20.

Key Laboratory of Integrated Pest Management in Crops, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

UDP-glycosyltransferases (UGTs) are major phase II enzymes involved in the metabolic detoxification of xenobiotics. In this study, two UGT-inhibitors, 5-nitrouracil and sulfinpyrazone, significantly increased sulfoxaflor toxicity against sulfoxaflor-resistant (Sul-R) , whereas there were no synergistic effects in susceptible (Sus) . The activity of UGTs in the Sul-R strain was significantly higher (1.35-fold) than that in the Sus strain. Further, gene expression determination demonstrated that 11 of 23 UGT genes were significantly upregulated (1.40- to 5.46-fold) in the Sul-R strain, among which the expression levels of , , , , and could be induced by sulfoxaflor. Additionally, knockdown of , , , and using RNA interference (RNAi) significantly increased sensitivity (1.57- to 1.76-fold) to sulfoxaflor in the Sul-R strain. These results suggested that UGTs might be involved in sulfoxaflor resistance in . These findings will facilitate further work to validate the functional roles of these UGT genes in sulfoxaflor resistance.
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http://dx.doi.org/10.1021/acs.jafc.1c00638DOI Listing
May 2021

IL-36α Exerts Proinflammatory Effects in Aspergillus fumigatus Keratitis of Mice Through the Pathway of IL-36α/IL-36R/NF-κB.

Invest Ophthalmol Vis Sci 2021 Apr;62(4):16

Department of Ophthalmology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong Province, China.

Purpose: To explore the role of IL-36α in corneas infected by Aspergillus fumigatus.

Methods: The experimental group was comprised of 15 corneas with fungal keratitis, and 15 healthy donor corneas were included in the control group. IL-36α was detected in normal and infected corneas of humans and C57BL/6 mice. Mice corneas were infected with A. fumigatus with or without pretreatment of recombinant mouse (rm) IL-36α and IL-36α neutralizing antibody (Ab). Primary macrophages were stimulated with 75% ethanol-killed A. fumigatus with or without pretreatment of rmIL-36α. The severity of the disease was documented by clinical score and photographs with a slit lamp. PCR, western blot, and immunostaining were used to determine the expression of IL-36α, IL-1β, IL-6, and TNF-α. Polymorphonuclear neutrophilic leukocyte infiltration was assessed by myeloperoxidase (MPO) assay and flow cytometry. Macrophage infiltration was tested by immunofluorescent staining and flow cytometry.

Results: IL-36α mRNA and protein were significantly elevated in human and mice corneas after infection. The rmIL-36α treatment of C57BL/6 mice increased clinical score, MPO levels, macrophage infiltration, and expression of the proinflammatory cytokines IL-1β, IL-6, and TNF-α compared with the infected controls, which showed a decrease due to IL-36α Ab treatment. In primary macrophages, IL-36α expression was also significantly increased by A. fumigatus. The rmIL-36α treatment upregulated IL-1β, IL-6, and phosphorylated nuclear factor (NF)-κB expression, which was significantly inhibited by rmIL-36Ra.

Conclusions: IL-36α act as a proinflammatory cytokine in A. fumigatus keratitis by promoting the infiltration of neutrophils and macrophages and increasing the secretion of IL-1β, IL-6, and TNF-α, in addition to regulating expression of phosphorylated NF-κB.
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http://dx.doi.org/10.1167/iovs.62.4.16DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8054633PMC
April 2021

Donor cell memory confers a metastable state of directly converted cells.

Cell Stem Cell 2021 Jul 12;28(7):1291-1306.e10. Epub 2021 Apr 12.

Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Münster 48149, Germany; Faculty of Medicine, University of Münster, Münster 48149, Germany. Electronic address:

Generation of induced oligodendrocyte progenitor cells (iOPCs) from somatic fibroblasts is a strategy for cell-based therapy of myelin diseases. However, iOPC generation is inefficient, and the resulting iOPCs exhibit limited expansion and differentiation competence. Here we overcome these limitations by transducing an optimized transcription factor combination into a permissive donor phenotype, the pericyte. Pericyte-derived iOPCs (PC-iOPCs) are stably expandable and functionally myelinogenic with high differentiation competence. Unexpectedly, however, we found that PC-iOPCs are metastable so that they can produce myelination-competent oligodendrocytes or revert to their original identity in a context-dependent fashion. Phenotypic reversion of PC-iOPCs is tightly linked to memory of their original transcriptome and epigenome. Phenotypic reversion can be disconnected from this donor cell memory effect, and in vivo myelination can eventually be achieved by transplantation of O4 pre-oligodendrocytes. Our data show that donor cell source and memory can contribute to the fate and stability of directly converted cells.
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http://dx.doi.org/10.1016/j.stem.2021.02.023DOI Listing
July 2021

Gut Microbial Metabolites in Parkinson's Disease: Implications of Mitochondrial Dysfunction in the Pathogenesis and Treatment.

Mol Neurobiol 2021 Aug 6;58(8):3745-3758. Epub 2021 Apr 6.

Department of Life Sciences, National Natural Science Foundation of China, Shuangqing Road 83, Beijing, 100085, China.

The search for therapeutic targets for Parkinson's disease (PD) is hindered by the incomplete understanding of the pathophysiology of the disease. Mitochondrial dysfunction is an area with high potential. The neurobiological signaling connections between the gut microbiome and the central nervous system are incompletely understood. Multiple lines of evidence suggest that the gut microbiota participates in the pathogenesis of PD. Gut microbial dysbiosis may contribute to the loss of dopaminergic neurons through mitochondrial dysfunction. The intervention of gut microbial metabolites via the microbiota-gut-brain axis may serve as a promising therapeutic strategy for PD. In this narrative review, we summarize the potential roles of gut microbial dysbiosis in PD, with emphasis on microbial metabolites and mitochondrial function. We then review the possible ways in which microbial metabolites affect the central nervous system, as well as the impact of microbial metabolites on mitochondrial dysfunction. We finally discuss the possibility of gut microbiota as a therapeutic target for PD.
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http://dx.doi.org/10.1007/s12035-021-02375-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8280023PMC
August 2021

Comparison of plasma and pulmonary availability of chlorogenic acid, forsythiaside A and baicalin after intratracheal and intravenous administration of Shuang-Huang-Lian injection.

J Ethnopharmacol 2021 Jun 1;274:114082. Epub 2021 Apr 1.

Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Institute of Medicinal Plant Development (IMPLAD), Chinese Academy of Medical Sciences & Peking Union Medical College, No. 151 Malianwa North Road, Haidian District, Beijing, 100193, PR China. Electronic address:

Ethnopharmacological Relevance: The off-label nebulization of Shuang-Huang-Lian (SHL) injection is often utilized to treat respiratory tract infections in China. However, the pulmonary biopharmaceutics of SHL was generally unknown, limiting the rational selection of therapeutic dose and dose frequency.

Aim Of The Study: To characterize the size distribution of nebulized aerosols and to compare the pharmacokinetics and the lung distribution of three chemical makers of SHL, chlorogenic acid (CHA), forsythiaside A (FTA) and baicalin (BC), after intratracheal and intravenous administration of SHL to rats.

Materials And Methods: The droplet size distribution profiles over nebulization process were dynamically monitored using a laser diffraction method whereas the levels of CHA, FTA and BC in plasma, lung tissues and bronchoalveolar lavage fluids (BALF) were determined by a validated LC-MS/MS assay. The pulmonary anti-inflammatory efficacy was evaluated using a lipopolysaccharide (LPS) induced lung inflammation model as indicated by the level of tumor necrosis factor-α (TNF-α) in BALF.

Results: The nebulization of SHL showed good inhalability and allowed the aerosols to reach the upper or lower respiratory tract dependent on the performance of selected nebulizers. Following intratracheal administration of SHL at different doses, CHA, FTA and BC were absorbed into the bloodstream with the mean absorption time being 67.5, 63.5 and 114 min, respectively, rendering mean absolute bioavailabilities between 42.4% and 61.4% roughly independent of delivered dose. Relative to the intravenous injection, the intrapulmonary delivery increased the lung-to-plasma concentration ratios of CHA, FTA and BC by more than 100 folds and markedly improved the lung availability by 563-676 folds, leading to enhanced and prolonged lung retention. The production of TNF-α in BALF was decreased by ~50% at an intratracheal dose of 125 μL/kg SHL to LPS-treated mice.

Conclusion: The nebulization delivery of SHL is a promising alternative to the intravenous injection for the treatment of respiratory tract infections.
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http://dx.doi.org/10.1016/j.jep.2021.114082DOI Listing
June 2021

Echocardiographic Characteristics and Outcome in Patients With COVID-19 Infection and Underlying Cardiovascular Disease.

Front Cardiovasc Med 2021 16;8:642973. Epub 2021 Mar 16.

Department of Ultrasound, Tongji Medical College, Union Hospital, Huazhong University of Science and Technology, Wuhan, China.

The cardiac manifestations of coronavirus disease 2019 (COVID-19) patients with cardiovascular disease (CVD) remain unclear. We aimed to investigate the prognostic value of echocardiographic parameters in patients with COVID-19 infection and underlying CVD. One hundred fifty-seven consecutive hospitalized COVID-19 patients were enrolled. The left ventricular (LV) and right ventricular (RV) structure and function were assessed using bedside echocardiography. Eighty-nine of the 157 patients (56.7%) had underlying CVD. Compared with patients without CVD, those with CVD had a higher mortality (22.5 vs. 4.4%, = 0.002) and experienced more clinical events including acute respiratory distress syndrome, acute heart injury, or deep vein thrombosis. CVD patients presented with poorer LV diastolic and RV systolic function compared to those without CVD. RV dysfunction (30.3%) was the most frequent, followed by LV diastolic dysfunction (9.0%) and LV systolic dysfunction (5.6%) in CVD patients. CVD patients with high-sensitivity troponin I (hs-TNI) elevation or requiring mechanical ventilation therapy demonstrated worsening RV function compared with those with normal hs-TNI or non-intubated patients, whereas LV systolic or diastolic function was similar. Impaired RV function was associated with elevated hs-TNI level. RV function and elevated hs-TNI level were independent predictors of higher mortality in COVID-19 patients with CVD. Patients with COVID-19 infection and underlying CVD displayed impaired LV diastolic and RV function, whereas LV systolic function was normal in most patients. Importantly, RV function parameters are predictive of higher mortality.
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http://dx.doi.org/10.3389/fcvm.2021.642973DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8008078PMC
March 2021

Dexmedetomidine ameliorates endotoxin-induced acute lung injury in vivo and in vitro by preserving mitochondrial dynamic equilibrium through the HIF-1a/HO-1 signaling pathway.

Redox Biol 2021 05 21;41:101954. Epub 2021 Mar 21.

Department of Anesthesiology and Critical Care Medicine, Tianjin Nankai Hospital, Tianjin Medical University, Tianjin, China. Electronic address:

Increasing lines of evidence identified that dexmedetomidine (DEX) exerted protective effects against sepsis-stimulated acute lung injury via anti-inflammation, anti-oxidation and anti-apoptosis. However, the mechanisms remain unclear. Herein, we investigated whether DEX afforded lung protection by regulating the process of mitochondrial dynamics through the HIF-1a/HO-1 pathway in vivo and in vitro. Using C57BL/6J mice exposed to lipopolysaccharide, it was initially observed that preemptive administration of DEX (50μg/kg) alleviated lung pathologic injury, reduced oxidative stress indices (OSI), improved mitochondrial dysfunction, upregulated the expression of HIF-1α and HO-1, accompanied by shifting the dynamic course of mitochondria into fusion. Moreover, HO-1-knockout mice or HO-1 siRNA transfected NR8383 cells were pretreated with HIF-1α stabilizer DMOG and DEX to validate the effect of HIF-1a/HO-1 pathway on DEX-mediated mitochondrial dynamics in a model of endotoxin-induced lung injury. We found that pretreatment with DEX and DMOG distinctly relieved lung injury, decreased the levels of mitochondrial ROS and mtDNA, reduced OSI, increased nuclear accumulation of HIF-1a and HO-1 protein in wild type mice but not HO-1 KO mice. Similar observations were recapitulated in NC siRNA transfected NR8383 cells after LPS stimulation but not HO-1 siRNA transfected cells. Concertedly, DEX reversed the impaired mitochondrial morphology in LPS stimulated-wild type mice or NC siRNA transfected NR8383 cells, upregulated the expression of mitochondrial fusion protein, while downregulated the expression of fission protein in HIF-1a/HO-1 dependent pathway. Altogether, our data both in vivo and in vitro certified that DEX treatment ameliorated endotoxin-induced acute lung injury by preserving the dynamic equilibrium of mitochondrial fusion/fission through the regulation of HIF-1a/HO-1 signaling pathway.
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http://dx.doi.org/10.1016/j.redox.2021.101954DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8027777PMC
May 2021

Carcinosarcoma and sarcomatoid carcinoma of the stomach: Two case reports.

Medicine (Baltimore) 2021 Mar;100(12):e24697

Department of Pathology, Lanzhou University Second Hospital, Lanzhou, Gansu Province, China.

Rationale: Carcinosarcoma and sarcomatoid carcinoma of the stomach are rare, malignant, and biphasic tumors with high mortality. The differential diagnosis of these 2 diseases remains challenging. In the present study, we present 2 cases of carcinosarcoma and sarcomatoid carcinoma of the stomach.

Patient Concerns: A 54-year-old woman was admitted with complaints of epigastric pain for 4 months, but she became serious for 10 days accompanied by melena. A 75-year-old man was admitted with complaints of epigastric pain for 1 month.

Diagnosis: The female had a Borrmann type III irregular ulcerative lesion (5.0 × 4.0 × 1.0 cm) originating from the gastric antrum. The male had Borrmann type I tumor polypoid exophytic (5.0 × 4.0 × 2.0 cm) in the fundus of stomach near the cardia. Both cases were identified as malignant neoplasms by endoscopic biopsy and further confirmed by performing laparoscopic proximal gastrectomy, esophagogastrostomy, and palliative distal subtotal gastrectomy. The postoperative histopathological morphology and immunohistochemistry studies revealed sarcomatoid carcinoma for the female and gastric carcinosarcoma for the male respectively.

Interventions: The female patient subsequently underwent laparoscopy-assisted radical distal gastrectomy for gastric cancer followed by systemic chemotherapy with oxaliplatin plus tegafur. The male patient underwent laparoscopic proximal gastrectomy and esophagogastrostomy were performed.

Outcomes: The female had a mixture of a little poorly-differentiated adenocarcinoma and abundant sarcomatoid spindle cell elements, and is still alive healthy up to date for 2 and a half years after surgery by phone follow-up. The male patient had both adenocarcinoma and fibrosarcoma in a single tumor, and died 1 month after the operation.

Lessons: The present study provides insight into the clinical findings, differential diagnosis, and prognosis of carcinosarcomas and sarcomatoid carcinomas of the stomach. More cases are needed for further studies in the future.
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http://dx.doi.org/10.1097/MD.0000000000024697DOI Listing
March 2021

Long-Term Fertilization History Alters Effects of Microplastics on Soil Properties, Microbial Communities, and Functions in Diverse Farmland Ecosystem.

Environ Sci Technol 2021 04 23;55(8):4658-4668. Epub 2021 Mar 23.

Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, 1799 Jimei Road, Xiamen 361021, China.

Microplastics (MPs) pollution has caused a threat to soil ecosystem diversity and functioning globally. Recently, an increasing number of studies have reported effects of MPs on soil ecosystems. However, these studies mainly focused on soil bacterial communities and a few limited functional genes, which is why MPs effects on soil ecosystems are still not fully understood. Fertilization treatment often coinsides with MPs exposure in practice. Here, we studied effects of an environmentally relevant concentration of polyethylene on soil properties, microbial communities, and functions under different soil types and fertilization history. Our results showed that 0.2% PE MPs exposure could affect soil pH, but this effect varied according to soil type and fertilization history. Long-term fertilization history could alter effects of MPs on soil bacterial and fungal communities in diverse farmland ecosystems ( < 0.05). Soil fungal communities are more sensitive to MPs than bacterial communities under 0.2% PE MPs exposure. MPs exposure has a greater impact on the soil ecosystem with a lower microbial diversity and functional genes abundance and increases the abundance of pathogenic microorganisms. These findings provided an integrated picture to aid our understanding of the impact of MPs on diverse farmland ecosystems with different fertilization histories.
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http://dx.doi.org/10.1021/acs.est.0c04849DOI Listing
April 2021

Printing High-Efficiency Perovskite Solar Cells in High-Humidity Ambient Environment-An In Situ Guided Investigation.

Adv Sci (Weinh) 2021 Mar 25;8(6):2003359. Epub 2021 Jan 25.

Department of Electronic and Information Engineering Research Institute for Smart Energy (RISE) The Hong Kong Polytechnic University Hung Hom Kowloon Hong Kong China.

Extensive studies are conducted on perovskite solar cells (PSCs) with significant performance advances (mainly spin coating techniques), which have encouraged recent efforts on scalable coating techniques for the manufacture of PSCs. However, devices fabricated by blade coating techniques are inferior to state-of-the-art spin-coated devices because the power conversion efficiency () is highly dependent on the morphology and crystallization kinetics in the controlled environment and the delicate solvent system engineering. In this study, based on the widely studied perovskite solution system dimethylformamide-dimethyl sulfoxide, air-knife-assisted ambient fabrication of PSCs at a high relative humidity of 55 ± 5% is reported. In-depth time-resolved UV-vis spectrometry is carried out to investigate the impact of solvent removal and crystallization rate, which are critical factors influencing the crystallization kinetics and morphology because of adventitious moisture. UV-vis spectrometry enables accurate determination of the thickness of the wet precursor film. Anti-solvent-free, high-humidity ambient coatings of hysteresis-free PSCs with s of 21.1% and 18.0% are demonstrated for 0.06 and 1 cm devices, respectively. These PSCs exhibit comparable stability to those fabricated in a glovebox, thus demonstrating their high potential.
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http://dx.doi.org/10.1002/advs.202003359DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7967091PMC
March 2021

Photoinduced formation of persistent free radicals, hydrogen radicals, and hydroxyl radicals from catechol on atmospheric particulate matter.

iScience 2021 Mar 16;24(3):102193. Epub 2021 Feb 16.

State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.

Catechol is speculated to be a potential precursor of environmentally persistent free radicals (EPFRs) in the atmosphere. EPFRs absorbed on PM have attracted public attention because their toxicity is similar to cigarette smoke. In this study, we found that catechol could produce EPFRs, which were oxygen-centered phenoxy and semiquinone radicals. These free radical species had half-lives of up to 382 days. CaO, CuO, and FeO markedly promoted EPFR formation from catechol. The valence states of Cu and Fe changed during the photochemical reactions of catechol but no valence state changed for Ca. Alkaline nature of CaO is possibly the key for promoting the free radical formations through acid-base reactions with catechol. In addition to hydroxyl free radicals, hydrogen free radicals and superoxide anions formed from the photochemical reactions of catechol were first discovered. This is of concern because of the adverse effects of these free radicals on human health.
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http://dx.doi.org/10.1016/j.isci.2021.102193DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7920856PMC
March 2021

Characteristics and reference values for cardiopulmonary exercise testing in the adult Chinese population - The Xiangya hospital exercise testing project (the X-ET project).

Int J Cardiol 2021 06 12;332:15-21. Epub 2021 Mar 12.

Division of Cardiac Rehabilitation, Department of Physical Medicine & Rehabilitation, Xiangya Hospital of Central South University, Changsha, Hunan Province, China; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital of Central South University, Changsha, Hunan Province, China. Electronic address:

Background: Reference values of cardiopulmonary exercise testing (CPX) vary with race/ethnicity. Chinese Americans are the fastest-growing racial/ethnic group in the United States. However, there is limited information about the reference values of cardiopulmonary exercise testing (CPX) variables in the Chinese population.

Methods: As part of the Xiangya Hospital Exercise Testing project (the X-ET project), this cross-sectional study screened 20,696 consecutive CPXs performed by 17,802 unique individuals at Xiangya Hospital of Central South University, China, from January 1, 2002, to December 31, 2019. A total of 964 unique healthy adults/tests (42% female) aged 49 ± 12 who completed a maximal ramp incremental CPX with cycle ergometry were included in this study. The reference values of primary CPX variables were expressed as the lower limit or upper limit of normal. Stepwise linear regression was used to fit the equations of key CPX variables. Predictive accuracy analysis for the equations with a comparison between present and previous studies were performed.

Results: Peak oxygen consumption (V̇O), carbon dioxide production, ventilation/min, work rate, and V̇O at the anaerobic threshold were regressed on age, height, weight, and sex. These predictive equations showed good in- and out-sample predictive accuracy. Comparison with prior research revealed that prediction equations of peak V̇O resultant from studies in which populations were entirely or primarily Caucasian had overestimated our subjects' actual values.

Conclusion: The reference values and predicted equations of CPX variables in this study may provide a more appropriate framework to interpret the response to maximal ramp incremental cycle ergometry in the Chinese adult population.
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http://dx.doi.org/10.1016/j.ijcard.2021.03.013DOI Listing
June 2021

Compositional Dependence of Curie Temperature and Magnetic Entropy Change in the Amorphous Tb-Co Ribbons.

Materials (Basel) 2021 Feb 20;14(4). Epub 2021 Feb 20.

Institute of Materials, Shanghai University, Shanghai 200072, China.

The Curie temperature () and magnetic entropy change (-Δ), and their relationship to the alloy composition of Tb-Co metallic glasses, were studied systematically in this paper. It was found that, in contrast to the situation in amorphous Gd-Co ribbons, the dependence of Tc on Tb content and the maximum -Δ vs. -2/3 plots in Tb-Co binary amorphous alloys do not follow a linear relationship, both of which are supposed to be closely related to the non-linear compositional dependence of Tb-Co interaction due to the existence of orbital momentum in Tb.
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http://dx.doi.org/10.3390/ma14041002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7924628PMC
February 2021

Identification of a novel genomic resistance island PmGRI1-STP3 and an SXT/R391 integrative conjugative element in Proteus mirabilis of swine origin in China.

J Glob Antimicrob Resist 2021 06 2;25:77-81. Epub 2021 Mar 2.

Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, College of Life Science, Sichuan University, Chengdu, China; Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Chengdu, Sichuan, China. Electronic address:

Objectives: This study aimed to determine the genetic environment of antimicrobial resistance genes in Proteus mirabilis strain STP3 isolated from a diarrhoeic pig on a swine farm in Sichuan Province, China.

Methods: Strain STP3 was subjected to antimicrobial susceptibility testing. Illumina MiSeq (200× coverage) and Nanopore PromethION (100× coverage) platforms were used for genome sequencing. A conjugation experiment was performed to determine the transferability and stability of antimicrobial resistance genes in this strain.

Results: The assembled circular genome of P. mirabilis STP3 was 4 115 975 bp with a GC content of 39.58%; no plasmid sequence was detected. A novel genomic resistance island (PmGRI1-STP3) and an SXT/R391 integrative conjugative element (ICE) variant (ICEPmiChnSTP3) were characterised in P. mirabilis STP3. PmGRI1-STP3 of 52.7 kb was located at the 3' end of tRNA-Sec and shared the greatest identity with PmGRI1-C55 (54% coverage, 99.99% identity). PmGRI1-STP3 carried 16 resistance genes, including the clinically important extended-spectrum β-lactamase (ESBL) gene bla. ICEPmiChnSTP3 was inserted into the prfC gene. It carried 18 resistance genes, including the rRNA methyltransferase gene cfr and the fluoroquinolone resistance gene aac(6')-Ib-cr. A class 2 integron (dfrA1-sat2-aadA1) was also identified on transposon Tn7. Mobilisation experiments indicated that ICEPmiChnSTP3 was conjugally mobilised to Escherichia coli. However, PmGRI1-STP3 appeared to lose its mobilisation ability.

Conclusion: The identification of two genomic islands (GIs) in this study suggested that genetic elements might be key mediators for resistance gene acquisition in P. mirabilis and that IS26-mediated rearrangements promote the diversity of GIs.
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http://dx.doi.org/10.1016/j.jgar.2021.02.018DOI Listing
June 2021

Involvement of Nrf-2/HO-1 pathway in sevoflurane-induced cognitive improvement in rats with traumatic brain injury.

Behav Brain Res 2021 May 23;405:113200. Epub 2021 Feb 23.

Department of Anesthesiology and Critical Care Medicine, Tianjin NanKai Hospital, Tianjin Medical University, Tianjin, 300100, China. Electronic address:

Traumatic brain injury (TBI) is an increasingly common emergency disease that usually leads to prolonged physical and cognitive impairments. In this study, we investigated if sevoflurane could induce cognitive improvement in TBI rats. Rats were subjected to head trauma induced by a fluid percussion device. A two-hour exposure to 3% sevoflurane was performed in a chamber immediately after TBI. Sevoflurane inhalation reduced the neurological and cognitive deficits induced by TBI with ameliorated synaptic injuries in the hippocampus. Moreover, after sevoflurane treatment, the expression of nuclear factor erythroid-2-related factor-2 (Nrf-2) and hemeoxygenase-1 (HO-1) in the hippocampus was enhanced 1 d after TBI and maintained at high levels 14 days later, and oxidative stress induced by TBI was inhibited. However, the HO-1 inhibitor, Zinc protoporphyrin (ZnPP), used to demonstrate the involvement of HO-1, suppressed the protective effect of sevoflurane. These results indicate that sevoflurane administered immediately after TBI may protect against TBI-induced synaptic and cognitive impairments by promoting the antioxidant Nrf-2/HO-1 pathway. Sevoflurane may be a promising anesthetic for patients with TBI.
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http://dx.doi.org/10.1016/j.bbr.2021.113200DOI Listing
May 2021

Prognostic Value of Right Ventricular Ejection Fraction Assessed by 3D Echocardiography in COVID-19 Patients.

Front Cardiovasc Med 2021 9;8:641088. Epub 2021 Feb 9.

Department of Ultrasound, Tongji Medical College, Union Hospital, Huazhong University of Science and Technology, Wuhan, China.

RVEF (right ventricular ejection fraction) measured by three-dimensional echocardiography (3DE) has been used in evaluating right ventricular (RV) function and can provide useful prognostic information in other various cardiovascular diseases. However, the prognostic value of 3D-RVEF in coronavirus disease 2019 (COVID-19) remains unknown. We aimed to investigate whether 3D-RVEF can predict the mortality of COVID-19 patients. A cohort of 128 COVID-19-confirmed patients who had undergone echocardiography were studied. Thirty-one healthy volunteers were also enrolled as controls. COVID-19 patients were divided into three subgroups (general, severe, and critical) according to COVID-19 severity-of-illness. Conventional RV structure and function parameters, RV free wall longitudinal strain (FWLS) and 3D-RVEF were acquired. RVFWLS was measured by two-dimensional speckle tracking echocardiography. RVEF was acquired by 3DE. Compared with controls, 2D-RVFWLS and 3D-RVEF were both significantly decreased in COVID-19 patients (-27.2 ± 4.4% vs. -22.9 ± 4.8%, < 0.001; 53.7 ± 4.5% vs. 48.5 ± 5.8%, < 0.001). Critical patients were more likely to have a higher incidence of acute cardiac injury and acute respiratory distress syndrome (ARDS), and worse prognosis than general and severe patients. The critical patients exhibited larger right-heart chambers, worse RV fractional area change (RVFAC), 2D-RVFWLS, and 3D-RVEF and higher proportion of pulmonary hypertension than general and severe patients. Eighteen patients died during a median follow-up of 91 days. The multivariate Cox regression analysis revealed the acute cardiac injury, ARDS, RVFAC, RVFWLS, and 3D-RVEF were independent predictors of death. 3D-RVEF (chi-square to improve 18.3; < 0.001), RVFAC (chi-square to improve 4.5; = 0.034) and 2D-RVFWLS (chi-square to improve 5.1; = 0.024) all provided additional prognostic value of higher mortality over clinical risk factors. Moreover, the incremental predictive value of 3D-RVEF was significantly ( < 0.05) higher than RVFAC and RVFWLS. 3D-RVEF was the most robust independent predictor of mortality in COVID-19 patients and provided a higher predictive value over conventional RV function parameters and RVFWLS, which may be helpful to identify COVID-19 patients at a higher risk of death.
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http://dx.doi.org/10.3389/fcvm.2021.641088DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7902006PMC
February 2021

Effect of broflanilide on the phytophagous mite Tetranychus urticae and the predatory mite Typhlodromips swirskii.

Pest Manag Sci 2021 Jun 15;77(6):2964-2970. Epub 2021 Mar 15.

College of Plant Protection, Nanjing Agricultural University, Nanjing, P.R. China.

Background: Two-spotted spider mite (TSSM), Tetranychus urticae Koch, is one of the most serious pests of agricultural crops. Broflanilide exhibits high lethality against various pests and has been marketed worldwide under the Vedira and Tenebenal brands in 2020. Nevertheless, little information has been reported about its effects on agricultural mites.

Results: Broflanilide displayed higher toxicity to TSSM eggs (24 h LC , 1.015 mg L ) and adult females (24 h LC , 2.062 mg L ) than commercial acaricides, including cyflumetofen, bifenazate, and profenofos. In contrast, the adverse effects of broflanilide on the predatory mite, Typhlodromips swirskii Athias-Henriot, was lower than those of fenpyroximate and abamectin. In the sublethal effect study, while adult females were treated with broflanilide, the number of eggs and longevity were reduced in LC and LC treatments; when eggs were treated with broflanilide, the egg duration and deutonymph duration were prolonged in LC treatment. A significant decrease in the total life span and duration and fecundity of adult females was observed in LC and LC treatments. Furthermore, the number of eggs per adult female was significantly reduced from 103.48 ± 3.69 in the control group to 69.42 ± 2.22 and 48.33 ± 1.75 in LC and LC treatments, respectively. In the greenhouse bioassay, broflanilide 5% suspension concentrate (MCI-8007) showed excellent acaricidal activity to TSSM, with 99.22% corrected control, compared with the MCI-8007 untreated group.

Conclusion: Our results indicated that broflanilide has a high acaricidal activity to TSSM and significant inhibition to fecundity of adult female, and could be considered as a potential alternative for TSSM management. © 2021 Society of Chemical Industry.
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http://dx.doi.org/10.1002/ps.6335DOI Listing
June 2021

Copper Ions Facilitate the Conjugative Transfer of SXT/R391 Integrative and Conjugative Element Across Bacterial Genera.

Front Microbiol 2020 2;11:616792. Epub 2021 Feb 2.

Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, China.

Copper can persist stably in the environment for prolonged periods. Except for inducing antibiotic resistance in bacteria, copper ions (Cu) can facilitate the horizontal transfer of plasmid DNA. However, whether and how Cu can accelerate the conjugative transfer of SXT/R391 integrative and conjugative element (ICE) is still largely unknown. In this study, ChSC1905, harboring an SXT/R391 ICE that carried 21 antibiotic resistance genes (ARGs), was used as a donor, and EC600 was used as a recipient. Cu, at subinhibitory and environmentally relevant concentrations (1-10 μmol/L), significantly accelerated the conjugative transfer of SXT/R391 ICE across bacterial genera (from to ) ( < 0.05). The combined analyses of phenotypic tests and genome-wide sequencing indicated that reactive oxygen species (ROS) production and cell membrane permeability were critical in the enhanced conjugative transfer of SXT/R391 ICE. Furthermore, the expression of genes related to cell adhesion and ATP synthesis was also significantly upregulated on exposure to Cu at a concentration of 5 μmol/L. This study clarified the potential mechanisms of Cu to promote the conjugative transfer of SXT/R391 ICE, revealing the potential risk imposed by Cu on the horizontal transfer of SXT/R391 ICE-mediated ARGs.
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http://dx.doi.org/10.3389/fmicb.2020.616792DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7884315PMC
February 2021

Formation of environmentally persistent free radicals from thermochemical reactions of catechol.

Sci Total Environ 2021 Jun 2;772:145313. Epub 2021 Feb 2.

State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; College of Resource and Environment, University of Chinese Academy of Sciences, Beijing 100049, China; School of Environment, Hangzhou Institute for Advanced Study, UCAS, Hangzhou 310000, China. Electronic address:

In many anthropogenic activities, catechol as a widespread organic chemical could be released and also environmentally persistent free radicals (EPFRs) can be unintentionally formed. However, the underlying links between EPFRs and the role of catechol as an important precursor are not well understood. In this study, EPFR formation from catechol during heating was monitored online by electron paramagnetic resonance spectroscopy. It was found that catechol can produce significant amounts of EPFRs via thermochemical reactions. The EPFR species formed from catechol on metal oxides were oxygen-centered phenoxy and semiquinone radicals. Their half-lives were evaluated to be in the range of 113-909 h. The promotional effects of CaO and CuO on EPFR formation from catechol were stronger than that of FeO. The promotional abilities and underlying mechanisms of various metal oxides in EPFR formation were clarified by X-ray photoelectron spectroscopy. Significant EPFR formation was observed during the cooling stage of a heating reaction system when CaO was used as the reaction medium. The obtained knowledge on the formation of EPFRs from catechol and the key factors involved will enable better control of the formation of EPFRs from anthropogenic activities.
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http://dx.doi.org/10.1016/j.scitotenv.2021.145313DOI Listing
June 2021

General versus local anesthesia for percutaneous radiofrequency ablation of hepatocellular carcinoma at unusual regions.

J Cancer Res Ther 2020 ;16(7):1686-1690

Department of Interventional Radiology, The First Medical Centre of Chinese PLA General Hospital, Beijing, China.

Aims: The aim of this study is to compare the efficacy and safety of percutaneous radiofrequency ablation (RFA) under general anesthesia or local anesthesia plus intraoperative analgesia in the treatment of hepatocellular carcinoma (HCC) at unusual regions.

Subjects And Methods: From July 2012 to October 2019, 83 consecutive patients with 107 HCC lesions were treated with interventional radiology therapy. The lesions were located at some unusual regions such as diaphragmatic surface, hepatic hilum, hepatic subcapsular region, tissues near inferior vena cava, and tissues near the colon. General anesthesia was applied in 57 cases (general anesthesia group) and local anesthesia plus intraoperative analgesia was used in 26 cases (local anesthesia group). All patients were treated with transcatheter arterial chemoembolization, followed immediately by RFA. The rate of tumor inactivation, time used for placing RF needles to the scheduled sites, pain score, and complications were analyzed.

Statistical Analysis Used: All continuous variables were tested for the normal/nonnormal distribution by Kolmogorov-Smirnov test. The t-test was used to analyze the normal distribution variables; the Mann-Whitney U-test was used to measure nonnormal distribution variables; and the Chi-square test for categorical variables. P < 0.05 was considered statistically significant.

Results: The treatments were successful in all patients, including 51 cases of complete response (CR) and 6 cases of partial response (PR) in the general anesthesia group and 18 cases of CR and 8 cases of PR in the local anesthesia group (P = 0.049). The time used for placing the needles to the scheduled sites was 1-5 min (mean 2 min) in the general anesthesia group and 2-9 min (mean 4 min) in the local analgesia group (P < 0.001). The pain scores ranged from 0 to 2 points (mean 1 point) in the general anesthesia group and 2-9 points (mean 5 points) in the local anesthesia group (P < 0.001). With regard to complications, seven cases had pneumothorax and four cases had slight hepatic subcapsular hemorrhage in the general anesthesia group and four cases of pneumothorax and three cases of slight hepatic subcapsular hemorrhage in the local anesthesia group, and the difference was not statistically significant between the two groups (P = 0.715).

Conclusions: For HCC located at unusual regions, general anesthesia is superior to local anesthesia plus intraoperative analgesia in percutaneous RFA in reducing the difficulty of the procedure and improving the safety of RFA.
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http://dx.doi.org/10.4103/jcrt.JCRT_1187_20DOI Listing
January 2020

Use of Bispectrum Analysis to Inspect the Non-Linear Dynamic Characteristics of Beam-Type Structures Containing a Breathing Crack.

Sensors (Basel) 2021 Feb 7;21(4). Epub 2021 Feb 7.

Faculty of Civil Engineering, University of Belgrade, Bulevar Kralja Aleksandra 73, 11000 Belgrade, Serbia.

A breathing crack is a typical form of structural damage attributed to long-term dynamic loads acting on engineering structures. Traditional linear damage identification methods suffer from the loss of valuable information when structural responses are essentially non-linear. To deal with this issue, bispectrum analysis is employed to study the non-linear dynamic characteristics of a beam structure containing a breathing crack, from the perspective of numerical simulation and experimental validation. A finite element model of a cantilever beam is built with contact elements to simulate a breathing crack. The effects of crack depth and location, excitation frequency and magnitude, and measurement noise on the non-linear behavior of the beam are studied systematically. The result demonstrates that bispectral analysis can effectively identify non-linear damage in different states with strong noise immunity. Compared with existing methods, the bispectral non-linear analysis can efficiently extract non-linear features of a breathing crack, and it can overcome the limitations of existing linear damage detection methods used for non-linear damage detection. This study's outcome provides a theoretical basis and a paradigm for damage identification in cracked structures.
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http://dx.doi.org/10.3390/s21041177DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7915679PMC
February 2021

Clinical manifestations and polysomnography-based analysis in nine cases of probable sporadic Creutzfeldt-Jakob disease.

Neurol Sci 2021 Feb 8. Epub 2021 Feb 8.

Department of Neurology, The First Hospital of Jilin University, 1 Xinming Street, Changchun, Jilin, China.

Purpose: To summarize the clinical characteristics of patients with sporadic Creutzfeldt-Jakob disease (sCJD), analyze its sleep disorder characteristics using polysomnography (PSG), and compare sleep disturbances with those of fatal familial insomnia (FFI).

Patients And Methods: We retrospectively reviewed the sleep disturbances; cerebrospinal fluid (CSF) protein 14-3-3 (CSF-14-3-3 protein); prion protein gene, PRNP; magnetic resonance imaging; and electroencephalogram (EEG) of nine sCJD patients RESULTS: Of the nine sCJD patients, six were positive for CSF-14-3-3 protein. In the eight patients who completed diffusion-weighted imaging, seven showed cortical "ribbons sign" and two showed high signal in the basal ganglia. All nine patients had an EEG, which showed an increase in background slow waves; moreover, four showed typical periodic sharp wave complexes. The codon diversity at position 129, 219 of nine patients were MM, EE. Almost all nine patients had sleep disturbances such as insomnia, hypersomnia, and periodic limb movement disorder (PLMD). Five patients completed PSG, which demonstrated severe sleep structure disorder, prolonged total waking time, significantly reduced sleep efficiency, and absent rapid eye movement in some severe patients.

Conclusion: Sleep disturbances are common in sCJD patients, manifested as insomnia, lethargy, and PLMD. The sCJD patients often demonstrate severe sleep structure disorder through PSG, which is similar to patients with FFI.
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http://dx.doi.org/10.1007/s10072-021-05102-8DOI Listing
February 2021

The expression and significance of leukemia inhibitory factor, interleukin-6 and vascular endothelial growth factor in Chinese patients with endometriosis.

Arch Gynecol Obstet 2021 Jul 8;304(1):163-170. Epub 2021 Feb 8.

Department of Gynaecology, Linyi Central Hospital, No.17 Jiankang Road, Yishui County, Linyi City, 276400, Shandong, China.

Objective: To observe the levels of leukemia inhibitory factor (LIF), interleukin-6 (IL-6) and vascular endothelial growth factor (VEGF) in blood, peritoneal fluid, ectopic endometrial tissue, and ectopic endometrial stromal cells of patients with endometriosis, and to compare expression of IL-6, LIF and VEGF expression between endometriotic and non-endometriotic patients underwent laparoscopic surgery.

Methods: Thirty-one patients who underwent laparoscopic surgery for endometriosis in our hospital from January 2018 to January 2020 were included in the observation group, and 32 patients who underwent laparoscopic surgery for uterine fibroids, ovarian serous cystadenoma, and ovarian teratomas, were included in the control group. The levels of LIF, IL-6 and VEGF in the blood and peritoneal fluid of the two groups of patients were detected. The levels of LIF, IL-6 and VEGF in ectopic endometrial tissue and self-paired eutopic endometrial tissue, ectopic endometrial stromal cells and self-paired eutopic endometrial stromal cells of patients in the observation group were detected. After treating the primary cultured ectopic endometrial stromal cells with LIF and IL-6 alone or in combination, the changes of VEGF mRNA of ectopic endometrial stromal cells and the VEGF levels in the supernatant were observed.

Results: The levels of LIF, IL-6 and VEGF in the blood and peritoneal fluid of the observation group were all higher than those of the control group (P < 0.05), and the levels of LIF, IL-6 and VEGF in the peritoneal fluid of the observation group were significantly higher than those in the blood (P < 0.05). In the observation group, the expression levels of LIF-mRNA and IL-6 mRNA in the ectopic endometrial tissue were higher than those in the self-paired eutopic endometrial tissues (P < 0.05), while the expression level of VEGF mRNA in the ectopic endometrial tissues was lower than that in the self-paired eutopic endometrial tissues (P < 0.05). Besides, the mRNA expression levels of LIF, IL-6 and VEGF in ectopic endometrial stromal cells of the observation group were all higher than those in the self-paired eutopic endometrial stromal cells (P < 0.05). After stimulating ectopic endometrial stromal cells with LIF, IL-6 and LIF + IL-6, respectively, the VEGF levels in the supernatant were all significantly higher than that in the blank control group (P < 0.05).

Conclusion: The LIF, IL-6 and VEGF levels in blood and peritoneal fluid were increased in patients with endometriosis, and increased LIF and IL-6 in ectopic endometriosis stromal cells can play a synergistic role in increasing the VEGF levels, which may be involved in the occurrence and development of endometriosis.
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http://dx.doi.org/10.1007/s00404-021-05980-5DOI Listing
July 2021

Macleaya cordata extract, an antibiotic alternative, does not contribute to antibiotic resistance gene dissemination.

J Hazard Mater 2021 06 30;412:125272. Epub 2021 Jan 30.

College of Environment, Zhejiang University of Technology, Hangzhou 310032, PR China. Electronic address:

The abuse of antibiotics and their associated health risks are receiving global attention. The use of antibiotic additives in fodder has been banned in the European Union since 2006 and in China since 2020. Antibiotic alternatives are being developed, but their risks to the soil ecosystem remain poorly understood. Here, we compared the effects of the antibiotic oxytetracycline (OTC10, 10 mg/kg) with those of a Macleaya cordata extract (MCE, 10 and 100 mg/kg), the major antibiotic substitute. All tested concentrations of MCE and OTC10 exerted slight effects on the soil microbiome, but OTC10 and MCE100 could interfere with the structures and functions of the gut microbiome and might thus affect the soil ecological functions of Enchytraeus crypticus. Furthermore, OTC10 exposure inevitably increased the antibiotic resistance gene (ARG) abundance by 213%, whereas MCE did not induce ARG dissemination, which explains why MCE is considered to be associated with a low ecological risk. Our research provides the first demonstration of the risks posed by antibiotic alternatives to soil animals from the perspective of environmental toxicology and explores the potential development of antibiotic alternatives associated with a low ecological risk from a new perspective.
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http://dx.doi.org/10.1016/j.jhazmat.2021.125272DOI Listing
June 2021
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