Publications by authors named "Cláudia Q da Rocha"

7 Publications

  • Page 1 of 1

Antimicrobial Activity of Extracts from the (Aubl).

Plants (Basel) 2021 Jul 19;10(7). Epub 2021 Jul 19.

Graduate Program in Microbial Biology, CEUMA University, Rua Josué Montello, 1-Renascença II, São Luís 65075-120, MA, Brazil.

(Aubl), commonly known as "mirim", is a plant of the Humiriaceae family, which consists of 39 species divided between eight genera: and . This study aimed to characterize extracts by LC-MS/MS and evaluate their antimicrobial potential through in vitro and in vivo assays. The leaves and stem bark of were collected and dried at room temperature and then ground in a knife mill. The extracts were prepared with organic solvents in order to increase the polarity index (hexane, ethyl acetate, and methanol). The antimicrobial effects of these extracts were evaluated against the following bacterial strains: ATCC 15313, Typhimurium ATCC 14028, and . The best activity was observed in the ethyl acetate (EALE = 780 µg/mL), methanol (MLE = 780 µg/mL), and hexane (HLE = 1560 µg/mL) leaf extracts against . Considering the results for both antimicrobial and antibiofilm activities, the EALE extract was chosen to proceed to the infection assays, which used larvae. The EALE treatment was able to extend the average lifespan of the larvae (6.5 days) in comparison to -infected larvae (1 day). Next, the samples were characterized by High-Performance Liquid Chromatography coupled to a mass spectrometer, allowing the identification of 11 substances, including seven flavonoids, substances whose antimicrobial activity is already well-reported in the literature. The number of bioactive compounds found in the chemical composition of emphasizes its significance in both traditional medicine and scientific research that studies new treatments based on substances from the Brazilian flora.
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http://dx.doi.org/10.3390/plants10071479DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8309364PMC
July 2021

Anthelmintic effect of essential rhizome oil from Hedychium coronarium Koenig (Zingiberaceae) introduced in Northeastern Brazil.

Acta Trop 2021 Jun 4;218:105912. Epub 2021 Apr 4.

Programa de Pós-Graduação em Química, Universidade Federal do Maranhão, 65080-805 São Luís, MA, Brazil. Electronic address:

Hedychium coronarium is native to Tropical Asia and has been introduced into several Brazilian biomes. Significant biological properties described for the essential oil (EO) from this species' rhizomes include antimicrobial, larvicidal, anti-inflammatory, antioxidant, phytotoxic, and anthelmintic activities. The primary constituents identified in this study by GC-MS in the EO were monoterpenes 1,8-cineole (33.5%), β-pinene (17.0%), α-terpineol (7.7%), α-pinene (7.3%), limonene (5.2%), and p-cymene (4.9%), comprising 75.6% of total oil compounds. The main monoterpenes' EO and standards were tested against N2 (susceptible) and UVR15 (resistant) adult nematode Caenorhabditis elegans strains, with varying dead rates in motility tests.. Nematocidal activity was not attributed to 1,8-cineole and β-pinene, the main H. coronarium rhizome oil components, as both exhibited an inhibitory concentration (IC) ≥ 5 mg/mL. On the other hand, the α-pinene (IC, 1.69 mg/mL) and (S)-(-)-limonene (IC, 1.66 mg/mL) standards demonstrated more efficient action than rhizome oil in motility tests, with significant adult C. elegans nematode mortality rates. These results support the hypothesis that the combination of H. coronarium EO constituents can be helpful as a nematicidal product, due to their synergistic action.
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http://dx.doi.org/10.1016/j.actatropica.2021.105912DOI Listing
June 2021

Production of Highly Active Antiparasitic Compounds from the Controlled Halogenation of the Crude Plant Extract.

J Nat Prod 2020 09 9;83(9):2631-2640. Epub 2020 Sep 9.

School of Pharmaceutical Sciences and Institute of Pharmaceutical Sciences of Western Switzerland (ISPSW), University of Geneva, CMU, Rue Michel Servet 1, 1211 Geneva 4, Switzerland.

Direct halogenation of phenolic compounds present in the CHCl extract of the roots of was investigated to enhance chemodiversity. The approach is based on eco-friendly reactions using NaBr, NaI, and NaCl in aqueous media to generate multiple "unnatural" halogenated natural products from crude extracts. The halogenation reactions, monitored by UHPLC-PDA-ELSD-MS, were optimized to generate mono-, di-, or trihalogenated derivatives. To isolate these compounds, the reactions were scaled up and the halogenated analogues were isolated by semipreparative HPLC-UV and fully characterized by NMR and HR-MS data. All of the original 16 halogenated derivatives were evaluated for their antiparasitic activities against the parasites and . Compounds presenting selective antiparasitic activities against one or both parasites with IC values comparable to the reference were identified.
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http://dx.doi.org/10.1021/acs.jnatprod.0c00433DOI Listing
September 2020

Investigation of U-251 cell death triggered by flavonoid luteolin: towards a better understanding on its anticancer property against glioblastomas.

Nat Prod Res 2020 Feb 21:1-7. Epub 2020 Feb 21.

Research Laboratory in Molecular Pharmacology of Bioactive Compounds, São Francisco University - Bragança Paulista, São Paulo, Brazil.

Recently, many studies have reported the anticancer properties of flavonoid luteolin against a variety of tumors, but there is still a lack in the description of its mechanism of action. In attempt to better contribute to the literature, we evaluated the antiproliferative activity of luteolin extracted by in a panel of tumor cell lines representative of six different tissues. Luteolin presented antiproliferative activity for all the assessed tumor cell lines, being glioblastoma the most sensitive one. This compound was able to inhibit U-251 cells migration and tumorigenesis. Besides, luteolin leads U-251 tumor cells to apoptosis death by depolarisation of the mitochondrial membrane, ERK proteins phosphorylation, cleavage of PARP and Caspase 9, further inducing DNA damage by H2AX phosphorylation, which had not yet been described for glioblastomas. Altogether, our results reaffirm luteolin as a potential therapeutic drug.
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http://dx.doi.org/10.1080/14786419.2020.1727470DOI Listing
February 2020

Antinociceptive and anti-inflammatory properties of 7-epiclusianone, a prenylated benzophenone from Garcinia brasiliensis.

Eur J Pharmacol 2011 Nov 10;670(1):280-5. Epub 2011 Sep 10.

Faculdade de Ciências Farmacêuticas, Universidade Federal de Alfenas-MG, 37130-000 Alfenas, Brazil.

7-Epiclusianone, a natural prenylated benzophenone, was extracted from Garcinia brasiliensis Planch. & Triana (Clusiaceae), a native plant commonly known as bacupari and used in traditional Brazilian medicine for the treatment of inflammatory diseases. As a result of the wide spectrum of biological activities attributed to polyisoprenylated benzophenones, the aim of this study was to evaluate the analgesic and anti-inflammatory effects of 7-epiclusianone using two animal models. Carrageenan-induced paw oedema and peritonitis were used to investigate the anti-inflammatory activity of 7-epiclusianone in rats. The acetic acid-induced writhing, formalin and hot-plate tests were used to investigate its antinociceptive activity in mice. At test doses of 5, 10 and 15 mg/kg p.o., 7-epiclusianone had an anti-inflammatory effect as demonstrated by the reduction of paw oedema induced by carrageenan and the inhibition of leukocyte recruitment into the peritoneal cavity. At the same doses, 7-epiclusianone inhibited nociception induced by an intraperitoneal injection of acetic acid, observed by the decrease in the number of writhing episodes. Additionally, 7-epiclusianone decreased licking time caused by a subplantar injection of formalin. Moreover, the hot plate test produced a significant increase in latency reaction, demonstrating an antinociceptive effect. The experimental data demonstrated that the polyisoprenylated benzophenone 7-epiclusianone has remarkable anti-inflammatory and antinociceptive activities.
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http://dx.doi.org/10.1016/j.ejphar.2011.08.032DOI Listing
November 2011

Anti-inflammatory and antinociceptive effects of Garcinia brasiliensis.

J Ethnopharmacol 2011 Jan 16;133(2):467-73. Epub 2010 Oct 16.

Faculty of Pharmaceutical Sciences, Federal University of Alfenas-MG, 37130-000 Alfenas, Brazil.

Aim Of The Study: In Brazilian folk medicine, the leaves of Garcinia brasiliensis are used to treat tumors, inflammation of the urinary tract and arthritis as well as to relieve pain. Nevertheless, scientific information regarding Garcinia brasiliensis is limited; there are no reports related to its possible anti-inflammatory and antinociceptive effects. This study employed in vivo inflammatory and nociceptive models to evaluate the scientific basis for the traditional use of Garcinia brasiliensis.

Materials And Methods: Carrageenan-induced paw edema, peritonitis and fibrovascular tissue growth induced by s.c. cotton pellet implantation were used to investigate the anti-inflammatory activity of Garcinia brasiliensis ethanolic extract (GbEE) in rats. Formalin and acetic acid-induced writhing tests were used to investigate the antinociceptive activity in mice.

Results: GbEE at test doses of 30-300 mg/kg p.o. clearly demonstrated anti-inflammatory effects by reduced paw edema induced by carrageenan, inhibited leukocyte recruitment into the peritoneal cavity, and in the model of chronic inflammation using the cotton pellet-induced fibrovascular tissue growth in rats, the GbEE significantly inhibited the formation of granulomatous tissue. The extracts at test doses of 30-300 mg/kg, p.o., clearly demonstrated antinociceptive activity, except for the first phase of the formalin test.

Conclusion: GbEE markedly demonstrated anti-inflammatory action in rats and antinociceptive activity in mice, which supports previous claims of the traditional use of species of the Garcinia genus for inflammation and pain.
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http://dx.doi.org/10.1016/j.jep.2010.09.036DOI Listing
January 2011

Anti-inflammatory and antinociceptive effects of Arrabidaea brachypoda (DC.) Bureau roots.

J Ethnopharmacol 2011 Jan 13;133(2):396-401. Epub 2010 Oct 13.

Laboratory of Physiology, Institute of Biomedical Sciences, Federal University of Alfenas, MG, Brazil.

Aim Of The Study: Arrabidaea brachypoda (DC.) Bureau has been used to relieve general pain, painful joints and kidney stones in Brazilian folk medicine. Nevertheless, scientific information regarding this species is scarce; there are no reports related to its possible analgesic and anti-inflammatory effects. This study was aimed at evaluating the traditional use of Arrabidaea brachypoda root using in vivo inflammatory and nociceptive models.

Materials And Methods: Carrageenan-induced paw edema, peritonitis and fibrovascular tissue growth induced by s.c. cotton pellet implantation were used to investigate the anti-inflammatory activity of Arrabidaea brachypoda roots ethanolic extract (AbEE) in rats. Formalin and acetic acid-induced writhing tests were used to investigate the antinociceptive activity in mice. High-performance liquid chromatography (HPLC) was used to determine the fingerprint chromatogram of AbEE.

Results: The AbEE at test doses of 30-300 mg/kg p.o. demonstrated anti-inflammatory effects. AbEE reduced paw edema induced by carrageenan, inhibited leukocyte recruitment into the peritoneal cavity and, in the model of chronic inflammation using the cotton pellet-induced fibrovascular tissue growth in rats, significantly inhibited the formation of granulomatous tissue. The extracts at test doses of 30-300 mg/kg p.o. clearly demonstrated antinociceptive activity, except during the first phase of the formalin test. The presence of quercetin and phenolic compounds in the extract Arrabidaea brachypoda was confirmed using HPLC.

Conclusion: Arrabidaea brachypoda ethanol extract markedly demonstrated anti-inflammatory action in rats and antinociceptive activity in mice, which supports the previous claims of traditional use.
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http://dx.doi.org/10.1016/j.jep.2010.10.009DOI Listing
January 2011
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