Publications by authors named "Cindy Richards"

13 Publications

  • Page 1 of 1

Stimulator of interferon genes (STING) is an essential proviral host factor for human rhinovirus species A and C.

Proc Natl Acad Sci U S A 2020 11 15;117(44):27598-27607. Epub 2020 Oct 15.

Lineberger Comprehensive Cancer Center, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599;

Human rhinoviruses (RVs) are positive-strand RNA viruses that cause respiratory tract disease in children and adults. Here we show that the innate immune signaling protein STING is required for efficient replication of members of two distinct RV species, RV-A and RV-C. The host factor activity of STING was identified in a genome-wide RNA interference (RNAi) screen and confirmed in primary human small airway epithelial cells. Replication of RV-A serotypes was strictly dependent on STING, whereas RV-B serotypes were notably less dependent. Subgenomic RV-A and RV-C RNA replicons failed to amplify in the absence of STING, revealing it to be required for a step in RNA replication. STING was expressed on phosphatidylinositol 4-phosphate (PI4P)-enriched membranes and was enriched in RV-A16 compared with RV-B14 replication organelles isolated in isopycnic gradients. The host factor activity of STING was species-specific, as murine STING (mSTING) did not rescue RV-A16 replication in STING-deficient cells. This species specificity mapped primarily to the cytoplasmic, ligand-binding domain of STING. Mouse-adaptive mutations in the RV-A16 2C protein allowed for robust replication in cells expressing mSTING, suggesting a role for 2C in recruiting STING to RV-A replication organelles. Palmitoylation of STING was not required for RV-A16 replication, nor was the C-terminal tail of STING that mediates IRF3 signaling. Despite co-opting STING to promote its replication, interferon signaling in response to STING agonists remained intact in RV-A16 infected cells. These data demonstrate a surprising requirement for a key host mediator of innate immunity to DNA viruses in the life cycle of a small pathogenic RNA virus.
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http://dx.doi.org/10.1073/pnas.2014940117DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7959528PMC
November 2020

Education, certification, and professional membership can help with career goals.

Authors:
Cindy A Richards

Nephrol News Issues 2016 May;30(5):17-8, 20

For the professional nephrology nurse, the interplay of certification, education, and professional association membership go hand-in-hand. The association provides the foundation, networking, and educational opportunities; certification validates skills and expertise; and education challenges and inspires the nurse to keep moving forward.
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May 2016

Nephrology Nursing and Education.

Authors:
Cindy Richards

Nephrol Nurs J 2016 Mar-Apr;43(2):93, 98

Professional nephrology nurses are responsible for their ongoing education and competency in their area of practice. ANNA has an additional opportunity for education for nephrology nurses at the 47th National Symposium to be held May 1-4, 2016, in Louisville, Kentucky. The Janel Parker Memorial Opening Session keynote speaker for the meeting will be Suzanne Miyamoto, PhD, RN, Senior Director of Government Affairs and Health Policy with the American Association of Colleges of Nursing. Her topic will be "Are We Practicing to the Fullest Extent? Licensure, Certification, and Education?" This session will help address educational competence in nephrology nursing.
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June 2016

Pediatric Renal Transplantation.

Authors:
Cindy Richards

Nephrol Nurs J 2016 Jan-Feb;43(1):35-7; quiz 38

Pediatric patients with end stage renal disease (ESRD) are not as prevalent as adults with ESRD, but the numbers are increasing each year. Medical management is the same for pediatric patients as it is with adults with ESRD: hemodialysis, peritoneal dialysis, no therapy, or transplantation. Among most pediatric nephrology centers, the goal for patients is to achieve transplantation.
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May 2016

Camps and Their Benefit to Pediatric Nephrology Patients.

Nephrol Nurs J 2016 Jan-Feb;43(1):27-9, 37; quiz 30

Children with end stage renal disease (ESRD) frequently miss great amounts of school due to hospitalizations and three-times-a week hemodialysis (if that is their modality); thus, they miss opportunities to be with their peers and learn normal social interactions with other students. Because of this lack of normal socialization, many children with ESRD are behind in development in contrast to their peers and need opportunities to enhance their growth and development. One way this can occur for children with ESRD is by providing them opportunities to attend age-appropriate and disease-appropriate camps.
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May 2016

Nephrology Nursing Across the Life Span.

Authors:
Cindy Richards

Nephrol Nurs J 2016 Jan-Feb;43(1):13-4

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May 2016

Nephrology Nursing and New Graduates.

Authors:
Cindy Richards

Nephrol Nurs J 2015 Nov-Dec;42(6):529, 537

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March 2016

Networking in Nephrology Nursing.

Authors:
Cindy Richards

Nephrol Nurs J 2015 Sep-Oct;42(5):429-30

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December 2015

Caring for Yourself as You Care for Chronically Ill Patients.

Authors:
Cindy Richards

Nephrol Nurs J 2015 Jul-Aug;42(4):329-30

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November 2015

Twenty-Eight Kidney Transplant Recipients and Counting! One Center's Kidney Transplant Chain Experience.

Nephrol Nurs J 2014 Nov-Dec;41(6):563-6; quiz 567

With over 101,000 people currently on the waiting list for a kidney transplant, innovative solutions are needed to expand the number of available donor organs. This article describes the experience of one transplant center in using living donor paired exchanges and donor swaps to result in a chain of 28 kidney transplant recipients in the past year.
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September 2015

Lifelong Learning and Nephrology Nursing.

Authors:
Cindy Richards

Nephrol Nurs J 2015 May-Jun;42(3):207-8

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August 2015

Mycobacterium tuberculosis gyrase inhibitors as a new class of antitubercular drugs.

Antimicrob Agents Chemother 2015 Apr 12;59(4):1868-75. Epub 2015 Jan 12.

Diseases of the Developing World, GSK, Severo Ochoa 2, Tres Cantos, Madrid, Spain.

One way to speed up the TB drug discovery process is to search for antitubercular activity among compound series that already possess some of the key properties needed in anti-infective drug discovery, such as whole-cell activity and oral absorption. Here, we present MGIs, a new series of Mycobacterium tuberculosis gyrase inhibitors, which stem from the long-term efforts GSK has dedicated to the discovery and development of novel bacterial topoisomerase inhibitors (NBTIs). The compounds identified were found to be devoid of fluoroquinolone (FQ) cross-resistance and seem to operate through a mechanism similar to that of the previously described NBTI GSK antibacterial drug candidate. The remarkable in vitro and in vivo antitubercular profiles showed by the hits has prompted us to further advance the MGI project to full lead optimization.
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http://dx.doi.org/10.1128/AAC.03913-14DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4356839PMC
April 2015

A rapid microtiter plate assay for measuring the effect of compounds on Staphylococcus aureus membrane potential.

J Microbiol Methods 2010 Nov 27;83(2):254-6. Epub 2010 Aug 27.

Antibacterial Discovery Performance Unit, Infectious Diseases Center of Excellence in Drug Discovery, GlaxoSmithKline Pharmaceuticals, Collegeville, PA 19426, United States.

We developed a homogenous microtiter based assay using the cationic dye 3, 3'-Diethyloxacarbocyanine iodide, DiOC2(3), to measure the effect of compounds on membrane potential in Staphylococcus aureus. In a screen of 372 compounds from a synthetic compound collection with anti-Escherichia coli activity due to unknown modes of action at least 17% demonstrated potent membrane activity, enabling rapid discrimination of nuisance compounds.
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http://dx.doi.org/10.1016/j.mimet.2010.08.012DOI Listing
November 2010