Publications by authors named "Chunyan Liu"

637 Publications

Anti-PADI4 antibody suppresses breast cancer by repressing the citrullinated fibronectin in the tumor microenvironment.

Biomed Pharmacother 2022 Jun 27;153:113289. Epub 2022 Jun 27.

Medical Research Center of the Affiliated Hospital of Qingdao University, Wutaishan Road 1677, Qingdao, Shandong 266000, PR China. Electronic address:

Background: PADI4, an enzyme catalyzing arginine residues to citrulline residues, is highly expressed in malignant tumors. This study prepared a monoclonal anti-human PADI4 antibody and investigated the therapeutic effect of the antibody on breast tumors and the functional mechanism.

Methods: After treatment with PADI4 antibody, the changes in tumor-bearing mice were examined by PET-CT, pathological assays, biochemical tests, routine blood tests, cytokine assays and metabolic assays. We used PADI4 recombinant protein to catalyze fibronectin (Fn) and then used citrullinated fibronectin (Cit-Fn) to culture MDA-MB-231 cells. We also treated Cit-Fn cultured cells with PADI4 antibody. The cultured cells were examined using cell proliferation, apoptosis, colony formation, migration and glycolic ATP production. Citrullination in the tumor tissues and peripheral blood was measured using Western blotting and ELISA, respectively.

Results: Following PADI4 antibody treatment, tumor growth was significantly suppressed, and the number of apoptotic cells in tumor tissues was increased. The citrullination level in peripheral blood and tumor tissues was decreased, EMT-related gene expression in tumors was also decreased, and the spontaneous movement of tumor-bearing mice was increased following treatment. Following antibody treatment, the serum concentrations of IL-10, IL-12p70, IL-23, ALT and AST were significantly decreased. MDA-MB-231 cells treated with Cit-Fn showed increased cell proliferation, cell migration, colony formation and glycolytic ATP production and decreased apoptosis. The growth and migration of MDA-MB-231 cells were reduced following PADI4 antibody treatment, and PADI4 antibody inhibited the citrullination of fibronectin in vitro.

Conclusions: The PADI4 antibody had a therapeutic effect on breast tumors by inhibiting the citrullination of fibronectin to change the tumor tissue microenvironment. PADI4 antibody is a potential means for tumor treatment.
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http://dx.doi.org/10.1016/j.biopha.2022.113289DOI Listing
June 2022

HLA-DR CD45RA- Tregs and CD28- Treg-like cells: Potential immunologic biomarkers for reproductive aging.

Am J Reprod Immunol 2022 Jun 30. Epub 2022 Jun 30.

Institute of Reproductive Health, Center for Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, P.R. China.

Problem: This study aimed to identify subsets of regulatory T cells (Tregs) associated with ovarian aging and determine whether they can be used as markers of reproductive aging.

Method: This prospective cohort study was conducted among women of reproductive age. Basic physiological characteristics, reproductive hormones, Treg cell subsets, and correlations between these parameters were assessed. The POSEIDON criteria was used to identify women with low reproductive potential.

Results: The percentages of HLA-DR CD45RA- Tregs and CD28- Treg-like cells significantly increased with age. Women between 40 and 49 years had significantly higher percentages of HLA-DR CD45RA- Tregs and CD28- Treg-like cells than those at 20-29, 30-34, and 35-39 years old. Age positively correlated with FSH levels and the percentages of HLA-DR CD45RA- Tregs and CD28- Treg-like cells, but inversely correlated with antral follicle count (AFC) and AMH levels. Interestingly, a positive correlation was found between the percentages of HLA-DR CD45RA- Tregs and FSH levels, whereas an inverse correlation was found between those of HLA-DR CD45RA- Tregs and AFC or AMH levels. Furthermore, a significant positive correlation was observed between the percentages of CD28- Treg-like cells and AFC. Based on POSEIDON criteria, women with the percentages of HLA-DR CD45RA- Tregs and CD28- Treg-like cells above reference value ranges were assigned to the low prognosis groups.

Conclusion: These findings suggest that HLA-DR CD45RA- Tregs and CD28- Treg-like cells can be used as immunologic markers of reproductive aging, which helps clinicians identify women with low reproductive potential and establish individualized therapeutic strategies. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1111/aji.13591DOI Listing
June 2022

To the Editor: Limitations and Alternative Solutions to a USMLE COMLEX-USA Concordance.

J Grad Med Educ 2022 06 13;14(3):353-354. Epub 2022 Jun 13.

Vice President, Psychometrics and Data Analysis, National Board of Medical Examiners.

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http://dx.doi.org/10.4300/JGME-D-22-00238.1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9200238PMC
June 2022

Molecular mechanism of ethylparaben on zebrafish embryo cardiotoxicity based on transcriptome analyses.

Sci Total Environ 2022 Jun 22:156785. Epub 2022 Jun 22.

Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, People's Republic of China. Electronic address:

Ethylparaben (EP), one of the parabens, a ubiquitous food and cosmetic preservatives, has caused widespread concern due to its health risks. Recently, studies have found that parabens exposure during pregnancy is negatively correlated with fetal and early childhood development. However, studies about EP on embryo development are few. In this study, the cardiotoxicity effects of EP concentrations ranging from 0 to 20 mg/L on zebrafish embryo development were explored. Results showed that EP exposure induce abnormal cardiac function and morphology, mainly manifested as pericardial effusion and abnormal heart rate in early-stage development of zebrafish embryos. Through transcriptome sequencing followed by Gene Ontology functional, and Kyoto Encyclopedia of Genes and Genomes pathway analysis, we further confirmed that EP exposure ultimately leads to cardiac morphologic abnormalities via the following three mechanisms: 1. Disruption of the retinoic acid signaling pathway related to original cardiac catheter development; 2. Inhibition of gene expression related to myocardial contraction; 3. Orientation development disturbance of heart tube. Moreover, O-Dianisidine staining, whole-mount in situ hybridization at 30 or 48 hours post fertilization (hpf) and hematoxylin-eosin staining results all confirmed the decreased heart's return blood volume, misoriented heart tubes toward either the right or the middle side, and heart loop defects. For the first time, we explored the mechanism by which EP exposure causes abnormal heart development in zebrafish embryos, laying the foundation for further revealing of the EP toxicity on embryonic development.
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http://dx.doi.org/10.1016/j.scitotenv.2022.156785DOI Listing
June 2022

Cognitive profile in mild cognitive impairment with Lewy bodies.

Singapore Med J 2022 06 24. Epub 2022 Jun 24.

Tianjin Key Laboratory of Cerebrovascular and of Neurodegenerative Diseases, Tianjin Dementia Institute, Department of Neurology, Tianjin Huanhu Hospital, Tianjin, China.

Introduction: This study aimed to elucidate the cognitive profile of patients with mild cognitive impairment with Lewy bodies (MCI-LB) and to compare it to that of patients with mild cognitive impairment due to Alzheimer's disease (MCI-AD).

Methods: Subjects older than 60 years with probable MCI-LB (n = 60) or MCI-AD (n = 60) were recruited. All patients were tested with Mini-Mental State Examination (MMSE) and Montreal Cognitive Assessment (MoCA) to assess their global cognitive profile.

Results: The MCI-AD and MCI-LB patients did not differ in total MMSE and MoCA scores. However, some sub-items in MMSE and MoCA were shown to be screening markers for differentiating MCI-LB from MCI-AD. In the visuoconstructive test, the total score and hands subitem score in the clock-drawing test were significantly lower in MCI-LB than in MCI-AD. As for the executive function, the 'animal fluency test', 'repeat digits backward test' and 'take paper by your right hand' in MMSE all showed lower scores in MCI-LB compared with MCI-AD. As for memory, 'velvet' and 'church' in MoCA and 'ball' and 'national flag' in MMSE had lower scores in MCI-AD than in MCI-LB.

Conclusion: This study presents the cognitive profile of patients with MCI-LB. In line with the literature on Dementia with Lewy bodies, our results showed lower performance on tests for visuoconstructive and executive function, whereas memory remained relatively spared in the early period.
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http://dx.doi.org/10.11622/smedj.2022085DOI Listing
June 2022

Ethanol extracts of Rhaponticum uniflorum (L.) DC inflorescence ameliorate LPS-mediated acute lung injury by alleviating inflammatory responses via the Nrf2/HO-1 signaling pathway.

J Ethnopharmacol 2022 Jun 20;296:115497. Epub 2022 Jun 20.

Inner Mongolia Key Laboratory of Mongolian Medicine Pharmacology for Cardio-Cerebral Vascular System, Tongliao, 028000, Inner Mongolia Autonomous Region, PR China; Affiliated Hospital of Inner Mongolia Minzu University, Tongliao, 028000, Inner Mongolia Autonomous Region, PR China. Electronic address:

Ethnopharmacological Relevance: Rhaponticum uniflorum (L.) DC is a member of the Compositae family. Loulu flowers (LLF) is the inflorescence of this plant, which is a commonly used Mongolian medicine for the treatment of inflammatory diseases due to its heat-clearing and detoxifying properties. It is used caused by. However, its anti-inflammatory mechanisms are not clear.

Aim Of This Study: We investigated whether ethanol extracts of LLF can alleviate LPS-induced acute lung injury and explored the mechanism involved.

Material And Methods: BALB/C mice were intragastrically administered with sodium carboxymethyl cellulose (0.5%, 1 mL/100 g) or ethanol extracts of LLF at a dose of 100, 200, and 400 mg/kg, once daily, for 3 days. Subsequently, mice models of acute lung injury were established by LPS and used for the determination of anti-inflammatory effects of LLF. After 6 h of treatment, mice were sacrificed to collect lung tissues and bronchoalveolar lavage fluid (BALF). H&E staining assay was performed on the tissues for pathological analysis. The ELISA test was conducted to measure NO, IL-6, TNF-α, MPO, SOD, CAT, MDA and GSH-PX levels. The expression level of proteins associated with the Nrf2/HO-1 and MAPK/NF-κB signaling pathways were determined using Western blot analysis. Levels of F4/80 and Nrf2 in lungs were quantified using immunohistochemistry.

Results: Oral administration of LLF extracts alleviated LPS-induced pathological alterations, reduced lung W/D weight ratio, decreased levels of TP, pro-inflammatory factors (TNF-α and IL-6), and NO in BALF. Pretreatment with LLF extract downregulated F4/80 expression in lung tissue and suppressed LPS-induced elevations in BALF and lung tissue levels of MPO. Moreover, treatment with LLF extract reduced the expression level of proteins associated with the MAPK signaling pathway (p-p38, p-JNK, p-ERK) and TLR4/NF-κB signaling pathways (TLR4, Myd88, p-IκB, p-p65). Moreover, LLF extract upregulated Nrf2, HO-1 and NQO1 protein levels, downregulated Keap1 protein level. Immunohistochemical analysis revealed that LLF reduced the LPS-induced increase in Nfr2 expression in lung tissues.

Conclusion: Ethanol extracts of LLF ameliorated LPS-induced acute lung injury by suppressing inflammatory response and enhancing antioxidation capacity, which correlated with the MAPK/NF-κB and Nfr2/HO-1 signaling pathways.
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http://dx.doi.org/10.1016/j.jep.2022.115497DOI Listing
June 2022

Elevated Plasma Orexin-A Levels in Prodromal Dementia with Lewy Bodies.

J Alzheimers Dis 2022 Jun;1(-1):1-12

Department of Neurology, Beijing Tiantan Hospital, Capital Medical University, China.

Background: Studies on plasma orexin-A levels in prodromal dementia with Lewy bodies (DLB) and the relationship with clinical manifestations are rare.

Objective: To assess plasma orexin-A levels and evaluate the correlation with clinical features in patients with mild cognitive impairment with Lewy bodies (MCI-LB) and DLB.

Methods: Plasma orexin-A levels were measured in 41 patients with MCI-LB, 53 with DLB, and 48 healthy controls (HCs). Informant-based history, neurological examinations, neuropsychological assessments, laboratory tests, and neuroimaging were collected and the correlation between orexin-A and various indicators evaluated.

Results: Plasma orexin-A levels in patients with MCI-LB (1.18±0.33 ng/mL, p = 0.014) or DLB (1.20±0.44 ng/mL, p = 0.011) were significantly higher than in HCs (1.02±0.32 ng/mL) and associated with gender and age. DLB patients with fluctuating cognition (FC) (1.01±0.23 versus 1.31±0.50, p = 0.007) or parkinsonism (PARK) (0.98±0.19 versus 1.25±0.47, p = 0.030) had significantly lower plasma orexin-A levels than subjects without FC or PARK. Plasma orexin-A levels were significantly negatively correlated with irritability and UPDRS-III scores and significantly positively correlated with disinhibition scores.

Conclusion: This is the first report in which elevated plasma orexin-A levels were observed in patients with MCI-LB or DLB. In addition, lower orexin-A levels were found in patients with DLB and FC or PARK compared with HCs. The plasma orexin-A levels were associated with the presence of core features and motor and neuropsychiatric symptoms in patients with MCI-LB and DLB.
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http://dx.doi.org/10.3233/JAD-220082DOI Listing
June 2022

The Responses of LR1 or Antibiotic on Intestinal Barrier Function and Microbiota in the Cecum of Pigs.

Front Microbiol 2022 2;13:877297. Epub 2022 Jun 2.

State Key Laboratory of Livestock and Poultry Breeding, Ministry of Agriculture Key Laboratory of Animal Nutrition and Feed Science in South China, Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangdong Laboratory for Lingnan Modern Agriculture, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou, China.

This study aimed to investigate responses of the or an antibiotic on cecal microbiota and intestinal barrier function in different stages of pigs. A total of 144 weaned pigs ( × × , 21 days of age) were randomly assigned to the control group (CON, fed with a basal diet), the antibiotic group (AO, fed with basal diet plus 100 mg/kg olaquindox and 75 mg/kg aureomycin), and the group (LR, fed with the basal diet + 5 × 10 CFU/kg LR1) throughout the 164-d experiment. A total of 45 cecal content samples (5 samples per group) from different periods (14th, 42th, and 164th days) were collected for 16S rRNA gene amplification. The results revealed that although LR and AO did not change the diversity of cecal microbiota in pigs, the abundance of some bacteria at the genus level was changed with age. The proportion of was increased by LR in early life, whereas it was decreased by AO compared with the control group. The relative abundance of was increased along with age. In addition, the gas chromatography results showed that age, not AO or LR, has significant effects on the concentrations of SCFAs in the cecum of pigs ( < 0.05). However, the mRNA expression of tight junction proteins () and were increased by AO in the cecum of pigs on day 14, while LR increased the mRNA expression of intestinal barrier-related proteins , , , , , and in the cecum of pigs on days 14 and 164 ( < 0.05). In conclusion, LR and AO have different effects on the intestinal barrier function of the cecum, and neither LR nor AO damaged the intestinal barrier function of pig cecum. In addition, LR and AO have little effects on cecal microflora in different stages of the pigs. The microflora and their metabolite SCFAs were significantly changed along with age. These findings provide important information to understand the homeostasis of the cecum of pigs after antibiotic or probiotic treatment.
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http://dx.doi.org/10.3389/fmicb.2022.877297DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9201390PMC
June 2022

is Related to the Type III Effector NopAA to Promote Nodulation in Soybean ().

Front Genet 2022 27;13:889795. Epub 2022 May 27.

College of Agriculture, Key Laboratory of Soybean Biology in Chinese Ministry of Education, Northeast Agricultural University, Harbin, China.

Type III effectors secreted by rhizobia regulate nodulation in the host plant and are important modulators of symbiosis between rhizobia and soybean (), although the underlying mechanisms are poorly understood. Here, we studied the type III effector NopAA in HH103, confirming its secretion into the extracellular environment under the action of genistein. The enzyme activity of NopAA was investigated , using xyloglucan and β-glucan as substrates. NopAA functions were investigated by the generation of a NopAA mutant and the effects of NopAA deficiency on symbiosis were analyzed. Soybean genes associated with NopAA were identified in a recombinant inbred line (RIL) population and their functions were verified. NopAA was confirmed to be a type III effector with glycosyl hydrolase activity, and its mutant did not promote nodulation. Quantitative trait locus (QTL) analysis identified 10 QTLs with one, (), found to be associated with NopAA and to positively regulate the establishment of symbiosis All these results support the hypothesis that type III effectors interact with host proteins to regulate the establishment of symbiosis and suggest the possibility of manipulating the symbiotic soybean-rhizobia interaction to promote efficient nitrogen fixation.
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http://dx.doi.org/10.3389/fgene.2022.889795DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9184740PMC
May 2022

The Relationship of Partial Pressure of Carbon Dioxide (PaCO) with Disease Severity Indicators Such as BODE and GOLD in Hospitalized COPD Patients.

Int J Clin Pract 2022 16;2022:4205079. Epub 2022 Mar 16.

Department of Respiratory Medicine, The Third Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China.

Purpose: This study aimed to investigate the relationship of partial pressure of carbon dioxide (PaCO) with BODE and GOLD in stable COPD subjects and to explore the predictive value of PaCO for severe COPD (BODE index score ≥5 or GOLD index score ≥3). . In total, 80 participants with COPD and free from other conditions affecting PaCO were recruited. Arterial blood gases, BODE, GOLD, SGRQ, lung function, and other data were collected. The BODE index was calculated, and patients were divided into two groups according to the BODE index and PaCO median, respectively. We used Pearson's correlation test and the receiver operating characteristic curves to evaluate the utility of PaCO. Besides, the univariate and multivariate logistic regression analyses were conducted to verify whether PaCO was an independent factor associated with BODE grades.

Results: COPD subjects with BODE ≥5 and GOLD ≥3 had significantly higher levels of PaCO ( = 0.004,  = 0.001, respectively). In the high PaCO group, patients underwent poorer outcomes than the low PaCO group. PaCO was negatively correlated with forced expiratory volume in 1 second in percent of the predicted value (FEV%) ( = -0.612,  < 0.001). The performance of PaCO levels in predicting BODE ≥5 and GOLD ≥3 was 0.748 and 0.755, respectively. The logistic regression analyses proved that PaCO was associated with BODE ≥5 in COPD patients (odds ratio = 1.160, 95% CI: 1.025-1.313,  = 0.019).

Conclusions: A higher level of PaCO was associated with a higher index for BODE or GOLD in COPD and had the predictive value for severe COPD.
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http://dx.doi.org/10.1155/2022/4205079DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9159220PMC
June 2022

An Examination of the Associations Among USMLE Step 3 Scores and Likelihood of Disciplinary Action in Practice.

Acad Med 2022 Jun 7. Epub 2022 Jun 7.

D.A. Johnson is chief assessment officer, Federation of State Medical Boards, Euless, Texas.

Purpose: As the last examination in the United States Medical Licensing Examination (USMLE) sequence, Step 3 provides a safeguard prior to physicians' entry into unsupervised practice. There is, however, little validity research focusing on Step 3 scores beyond examining its associations with other educational and professional assessments thought to cover similar content. This study examines the associations between Step 3 scores and subsequent receipt of disciplinary action taken by state medical boards for problematic behavior in practice. It analyzes Step 3 total, Step 3 computer-based case simulation (CCS), and Step 3multiple-choice question (MCQ) scores.

Method: The final sample included 275,392 board-certified physicians who graduated from MD-granting medical schools and who passed Step 3 between 2000 and 2017. Cross-classified multilevel logistic regression models were used to examine the effects of Step 3 scores on the likelihood of receiving a disciplinary action, controlling for other USMLE scores and accounting for jurisdiction and specialty.

Results: Results showed that physicians with higher Step 3 total, CCS, and MCQ scores tended to have lower chances of receiving a disciplinary action, after accounting for other USMLE scores. Specifically, a 1-standard-deviation increase in Step 3 total, CCS, and MCQ score was associated with a 23%, 11%, and 17% decrease in the odds of receiving a disciplinary action, respectively.The effect of Step 2 CK score on the likelihood of receiving a disciplinary action was statistically significant, while the effect of Step 1 score became statistically non-significant when other Step scores were included in the analysis.

Conclusions: Physicians who perform better on Step 3 are less likely to receive a disciplinary action from a state medical board for problematic behavior in practice. These findings provide some validity evidence for the use of Step 3 scores when making medical licensure decisions in the United States.
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http://dx.doi.org/10.1097/ACM.0000000000004775DOI Listing
June 2022

Gut Microbiome and Plasma Metabolomic Analysis in Patients with Myelodysplastic Syndrome.

Oxid Med Cell Longev 2022 9;2022:1482811. Epub 2022 May 9.

Department of Hematology, Tianjin Medical University General Hospital, China.

Myelodysplastic syndrome (MDS) is a heterogeneous group of clonal hematopoietic stem cell disorders. Studies have shown the involvement of an abnormal immune system in MDS pathogenesis. The gut microbiota are known to influence host immunity and metabolism, thereby contributing to the development of hematopoietic diseases. In this study, we performed gut microbiome and plasma metabolomic analyses in patients with MDS and healthy controls. We found that patients with MDS had a different gut microbial composition compared to controls. The gut microbiota in MDS patients showed a continuous evolutionary relationship from the phylum to the species level. At the species level, the abundance of , , , and increased, while that of decreased in MDS patients compared to controls. Moreover, abundance of bacterial genera correlated with the percentage of lymphocyte subsets in patients with MDS. Metabolomic analysis showed that the concentrations of hypoxanthine and pyroglutamic acid were increased, while that of 3a,7a-dihydroxy-5b-cholestan was decreased in MDS patients compared to controls. In conclusion, gut microbiome and plasma metabolomics are altered in patients with MDS, which may be involved in the immunopathogenesis of the disease.
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http://dx.doi.org/10.1155/2022/1482811DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9110251PMC
May 2022

Clinical analysis of a case of recurrent myocardial infarction treated with combined therapy of Rivaroxaban and evolocumab.

Minerva Med 2022 May 18. Epub 2022 May 18.

Department of Cardiology, The Fourth People's Hospital of Jinan, Jinan, China -

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http://dx.doi.org/10.23736/S0026-4806.22.08152-6DOI Listing
May 2022

Cofilin-1 participates in the hyperfunction of myeloid dendritic cells in patients with severe aplastic anaemia.

J Cell Mol Med 2022 Jun 17;26(12):3460-3470. Epub 2022 May 17.

Department of Hematology, Tianjin Medical University General Hospital, Tianjin, China.

Cofilin-1 interacts with actin to regulate cell movement. The importance of cofilin-1 in immunity has been established, and its involvement in a number of autoimmune diseases has been confirmed. However, its role in severe aplastic anaemia (SAA) remains elusive. Thus, the aim of the current study was to investigate the role of cofilin-1 in patients with SAA. Flow cytometry, Western blotting and real-time quantitative reverse transcription-polymerase chain reaction were performed to detect the mRNA and protein expression of cofilin-1 in myeloid dendritic cells (mDCs) from patients with SAA. The expression of cofilin-1 was then suppressed via siRNA, and its effects on mDCs and downstream effector T-cell function were evaluated. Cofilin-1 expression was higher in mDCs from patients with SAA and correlated with routine blood and immune indexes. Moreover, cofilin-1 knockdown in mDCs from patients with SAA reduced their phagocytic capacity, migration capacity, and CD86 expression through F-actin remodelling, downregulating the stimulatory capacity of mDCs on CD4 and CD8 T lymphocytes. Collectively, these findings indicate that cofilin-1 participates in the hyperfunction of mDCs in patients with SAA and that the downregulation of cofilin-1 in mDCs from patients with SAA could be a novel treatment approach for SAA.
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http://dx.doi.org/10.1111/jcmm.17359DOI Listing
June 2022

Abnormal Proteomics Profile of Plasma Reveals the Immunological Pathogenesis of Severe Aplastic Anemia.

Dis Markers 2022 6;2022:3700691. Epub 2022 May 6.

Department of Hematology, Tianjin Medical University General Hospital, 154 Anshan Street, Heping District, Tianjin 300052, China.

Severe aplastic anemia (SAA) is an immune-mediated bone marrow failure characterized by pancytopenia. This study was aimed at uncovering proteins of plasma that were differentially expressed in SAA patients. 8 SAA patients and 8 health controls were enrolled and detected by data independent acquisition (DIA) technology. 154 differential expression proteins (DEPs) in plasma of SAA patients were identified. GO and KEGG analyses indicated DEPs were mainly involved in the immune system process. Specifically, C-C motif chemokine 18 (CCL18), matrix metalloproteinase-3 (MMP3), histidine-rich glycoprotein (HRG), and lactotransferrin (lactoferrin (Lf)) may play an important role in the immune pathogenesis of SAA. CCL18, MMP3, HRG, and Lf might be potential biomarkers for SAA.
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http://dx.doi.org/10.1155/2022/3700691DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9106528PMC
May 2022

Multi-Omics Techniques for Soybean Molecular Breeding.

Int J Mol Sci 2022 Apr 30;23(9). Epub 2022 Apr 30.

College of Agriculture, Northeast Agricultural University, Harbin 150030, China.

Soybean is a major crop that provides essential protein and oil for food and feed. Since its origin in China over 5000 years ago, soybean has spread throughout the world, becoming the second most important vegetable oil crop and the primary source of plant protein for global consumption. From early domestication and artificial selection through hybridization and ultimately molecular breeding, the history of soybean breeding parallels major advances in plant science throughout the centuries. Now, rapid progress in plant omics is ushering in a new era of precision design breeding, exemplified by the engineering of elite soybean varieties with specific oil compositions to meet various end-use targets. The assembly of soybean reference genomes, made possible by the development of genome sequencing technology and bioinformatics over the past 20 years, was a great step forward in soybean research. It facilitated advances in soybean transcriptomics, proteomics, metabolomics, and phenomics, all of which paved the way for an integrated approach to molecular breeding in soybean. In this review, we summarize the latest progress in omics research, highlight novel findings made possible by omics techniques, note current drawbacks and areas for further research, and suggest that an efficient multi-omics approach may accelerate soybean breeding in the future. This review will be of interest not only to soybean breeders but also to researchers interested in the use of cutting-edge omics technologies for crop research and improvement.
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http://dx.doi.org/10.3390/ijms23094994DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9099442PMC
April 2022

COVID-19 in pediatric kidney transplantation: a follow-up report of the Improving Renal Outcomes Collaborative.

Pediatr Nephrol 2022 May 11. Epub 2022 May 11.

Cincinnati Children's Hospital Medical Center, 3333 Burnet Ave, MLC 7022, Cincinnati, OH, 45229, USA.

Background: We report follow-up data from an ongoing prospective cohort study of COVID-19 in pediatric kidney transplantation through the Improving Renal Outcomes Collaborative (IROC).

Methods: Patient-level data from the IROC registry were combined with testing, indication, and outcomes data collected to describe the epidemiology of COVID testing, treatment, and clinical outcomes; determine the incidence of a positive COVID-19 test; describe rates of COVID-19 testing; and assess for clinical predictors of a positive COVID-19 test.

Results: From September 2020 to February 2021, 21 centers that care for 2690 patients submitted data from 648 COVID-19 tests on 465 patients. Most patients required supportive care only and were treated as outpatients, 16% experienced inpatient care, and 5% experienced intensive care. Allograft complications were rare, with acute kidney injury most common (7%). There was 1 case of respiratory failure and 1 death attributed to COVID-19. Twelve centers that care for 1730 patients submitted complete testing data on 351 patients. The incidence of COVID-19 among patients at these centers was 4%, whereas the incidence among tested patients was 19%. Risk factors to predict a positive COVID-19 test included age > 12 years, symptoms consistent with COVID-19, and close contact with a confirmed case of COVID-19.

Conclusions: Despite the increase in testing and positive tests over this study period, the incidence of allograft loss or death related to COVID-19 remained extremely low, with allograft loss or death each occurring in < 1% of COVID-19-positive patients and in less than < 0.1% of all transplant patients within the IROC cohort. A higher resolution version of the Graphical abstract is available as Supplementary information.
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http://dx.doi.org/10.1007/s00467-022-05570-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9090538PMC
May 2022

Synergistic anti-inflammatory effects of peimine, peiminine, and forsythoside a combination on LPS-induced acute lung injury by inhibition of the IL-17-NF-κB/MAPK pathway activation.

J Ethnopharmacol 2022 Sep 6;295:115343. Epub 2022 May 6.

Key Laboratory of Natural Medicines of the Changbai Mountain, Ministry of Education, College of Pharmacy, Yanbian University, Yanji, 133002, China. Electronic address:

Ethnopharmacological Relevance: Forsythia suspensa (Thunb.) Vahl and Fritillaria thunbergii Miq are traditional Chinese medicines that exhibit the ability to clear heat and toxic material effects. In China, the combination of these two medicines is widely used to treat mucopurulent sputum and bloody phlegm, arising due to phlegm-heat obstruction in respiratory diseases. However, very limited information is available regarding the combined anti-inflammatory effect of important effective components of Forsythia suspensa (Thunb.) Vahl and Fritillaria thunbergii Miq, namely peimine, peiminine, and forsythoside A.

Aim Of This Study: To investigate synergistic anti-inflammatory effects of combined administration of peimine, peiminine, and forsythoside A on LPS-induced acute lung injury compared to combined administration of two compounds or individual administration, and unravel the underlying mechanism.

Material And Methods: In the present study, male BALB/c mice received an oral dosage of sodium carboxymethylcellulose (CMC-Na) (0.5%, 1 mL/100 g), peimine, peiminine, forsythoside A, peimine + forsythoside A, peiminine + forsythoside A, and peimine + peiminine + forsythoside A (suspended in CMC-Na; 0.5%), once daily for 7 days. Subsequently, intratracheal instillation of LPS was applied to establish acute lung injury model. After 6 h of administration, the mice were sacrificed, and bronchoalveolar lavage fluid (BALF) and lung tissues were collected. These samples were further used to determine lung W/D (wet/dry) weight ratio, total protein (TP) levels, inflammatory cytokines (IL-6, TNF-α, IL-1β, and IL-17), and expression of proteins involved in TLR4/MAPK/NF-κB pathway and IL-17 pathway. Further, tissue sections were subjected to H&E staining to assess the pathological alterations induced by LPS. The expression of IL-6 and TNF-α proteins in lung tissues was also analyzed using immunohistochemical staining.

Results: A synergistic anti-inflammatory effect of peimine, peiminine, and forsythoside A was observed when administered in combination to LPS-induced acute lung injury. The combined administration of peimine, peiminine, and forsythoside A had a strongly inhibitory effects on the W/D weight ratio, total protein (TP) level and the inflammatory cytokines (TNF-α, IL-6, IL-1β, and IL-17) level in acute lung injury mice, compared to combined administration of two compounds or individual administration. The infiltration of inflammatory cells and thickened bronchoalveolar walls induced by LPS were also ameliorated through the combined administration of peimine, peiminine, and forsythoside A. More importantly, the upregulation of protein related to TLR4/MAPK/NF-κB signaling pathway and the activation of IL-17 were significantly suppressed by pretreatment with each of the three compounds alone, while the effects of individual compounds were synergistically augmented by the combined pretreatment of these three compounds.

Conclusion: The combined administration of peimine, peiminine, and forsythoside A ameliorated inflammatory response in acute lung injury mice induced by LPS in a synergistic manner, the mechanism may be related to the dampening of the TLR4/MAPK/NF-κB signaling pathway and IL-17 activation.
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http://dx.doi.org/10.1016/j.jep.2022.115343DOI Listing
September 2022

Multi-Platform Omics Analysis Reveals Molecular Signatures for Pathogenesis and Activity of Systemic Lupus Erythematosus.

Front Immunol 2022 19;13:833699. Epub 2022 Apr 19.

Department of Otolaryngology, Head and Neck Surgery, Children's Hospital Affiliated Capital Institute of Pediatrics, Beijing, China.

Systemic lupus erythematosus (SLE) is a complex autoimmune disease with heterogeneous clinical manifestations and the pathogenesis of SLE is still unclear. Various omics results have been reported for SLE, but the molecular hallmarks of SLE, especially in patients with different disease activity, using an integrated multi-omics approach have not been fully investigated. Here, we collected blood samples from 10 healthy controls (HCs) and 40 SLE patients with different clinical activity including inactive (IA), low activity (LA), and high activity (HA). Using an integrative analysis of proteomic, metabolomic and lipidomic profiles, we report the multi-omics landscape for SLE. The molecular changes suggest that both the complement system and the inflammatory response were activated in SLEs and were associated with disease activity. Additionally, activation of the immunoglobulin mediated immune response were observed in the LA stage of the disease, however this immune response was suppressed slightly in the HA stage. Finally, an imbalance in lipid metabolism, especially in sphingolipid metabolism, accompanied with dysregulated apolipoproteins were observed to contribute to the disease activity of SLE. The multi-omics data presented in this study and the characterization of peripheral blood from SLE patients may thus help provide important clues regarding the pathogenesis of SLE.
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http://dx.doi.org/10.3389/fimmu.2022.833699DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9063006PMC
April 2022

A perspective of ecological civilization: research on the spatial coupling and coordination of the energy-economy-environment system in the Yangtze River Economic Belt.

Environ Monit Assess 2022 May 6;194(6):403. Epub 2022 May 6.

College of Foreign Languages & Cultures, Chengdu University of Technology, Chengdu b, 610059, China.

The concept of sustainable economic development as the core promotes socio-economic progress and rapid economic development. Nevertheless, in the new era of China, the contradiction between socio-economic development and environmental sustainability is still prominent. In order to deal with the coordinated development relationship among regional economy, energy, and environment, this paper constructs an energy-economy-environment (3E) index system from the perspective of green development and ecological integrity. On this basis, the distance-based coupling coordinated degree (CCD) model and dynamically comprehensive coordination degree model were used to evaluate the coupling coordinated development levels of 11 provinces (cities) along the Yangtze River Economic Belt (YREB) from 2008 to 2017. Then, a spatial econometric model was used to explore the spatial effects of the regional 3E system of the YREB. The results indicated that (1) the overall coordination of the 3E system in the provinces (cities) along the YREB shows an increasing trend. However, change paths varied with the provinces (cities) due to the influence of regional policies; (2) the CCD of the 3E system in the YREB differed spatially significantly, showing a stepped distribution of "high in the east and low in the west"; (3) the 3E system in the YREB had a positive spatial correlation and showed the characteristics of aggregation, with Jiangsu and Zhejiang provinces mainly showing "high-high" agglomeration and Sichuan showing "high-low" aggregation, while the rest of the provinces had the characteristics of random distribution in terms of spatial effects. The research process and results show that combining the ecological civilization concept and the CCD model can provide the vision and indicators for evaluating and researching the regional 3E system.
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http://dx.doi.org/10.1007/s10661-022-10065-0DOI Listing
May 2022

Identification of potential pathogenic genes for severe aplastic anemia by whole-exome sequencing.

J Clin Lab Anal 2022 May 18;36(5):e24438. Epub 2022 Apr 18.

Department of Hematology, Tianjin Medical University General Hospital, Tianjin, China.

Background: Severe aplastic anemia (SAA) is a syndrome of severe bone marrow failure due to hyperfunction of CD8+ T cells. While, the genetic background of SAA is still unknown. In this study, we tried to explore the possible genetic variants in CD8+ T cells of SAA patients.

Methods: We performed whole-exome sequencing (WES) in CD8+ T cells of 4 SAA patients and 7 normal controls. The mutations that existed in SAA but not in NCs were identified as candidate genes. Then, we compared them with genes in the enriched KEGG pathway of differently expressed genes (DEGs) from previous RNA-seq. After analyzing the types of mutations, we identified possible pathogenic genes and validated them by RT-PCR. Finally, we compared them with the autoimmune disease-related genes in DisGeNET database to select the most possible pathogenic genes.

Results: We found 95 candidate mutant genes in which, 4 possible pathogenic genes were identified: PRSS1, KCNJ18, PRSS2, and DGKK. RT-PCR results showed that compared with NCs, PRSS1 and KCNJ18 mRNA expression was significantly increased in SAA patients (p < 0.05), PRSS2 was also increased in SAA patients but without statistical difference, and DGKK gene could not be detected by RT-PCR in SAA patients. In addition, PRSS1 was associated with autoimmune diseases from the DisGeNET database.

Conclusion: The mutations of PRSS1, KCNJ18, PRSS2, and DGKK, especially PRSS1 in CD8+T cells, may be involved in the immune pathogenesis of SAA.
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http://dx.doi.org/10.1002/jcla.24438DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9102512PMC
May 2022

NOD1 activation promotes cell apoptosis in papillary thyroid cancer.

Pathol Res Pract 2022 May 11;233:153880. Epub 2022 Apr 11.

Department of Stomatology, Affiliated Hospital of Jiangnan University, Wuxi 214062, Jiangsu Province, China. Electronic address:

Background: NOD1 can promote or inhibit different types of cancers, suggesting that NOD1 functions in the progression of cancers dependent on the tumour environment. However, the expression and mechanisms of NOD1 during papillary thyroid carcinoma (PTC) progression remain unclear.

Methods: To investigate the role of NOD1 in PTC development and apoptosis, we detected NOD1 expression in three cell lines and surgical specimens from patients with PTC using immunohistochemistry, quantitative real-time PCR (Q-PCR) and Western blotting; we studied the biological functions of NOD1 by loss-of-function analysis of TPC-1 and BCPAP cells and the effect of NOD1 on the progression of PTC in terms of cell proliferation and apoptosis induced by tumour necrosis factor alpha (TNF-α), Fas ligand (Fas L), tumour necrosis factor-related apoptosis-inducing ligand (TRAIL) or Ala-γGlu-diaminopimelic acid (TriDAP) in the presence of cycloheximide (CHX) and determined its underlying molecular mechanism using PTC cell lines in vitro and mouse xenograft models in vivo.

Results: Increased expression of NOD1 is correlated with an improved prognosis in thyroid cancer patients. We also found that NOD1 expression was markedly upregulated in PTC cells compared to normal epithelial cells. NOD1 knockdown significantly promoted the proliferation of PTC cells in vitro, while activation of NOD1 signalling promoted PTC cell apoptosis; NOD1-induced apoptosis depends on the activation of caspase-3 and caspase-9 although the RIP2/TAK1 and MAPK pathways in PTC cells.

Conclusions: This study demonstrates that NOD1 is a predictive biomarker for PTC and that PTC cell apoptosis is induced by activation of NOD1 via the RIP2/TAK1 and MAPK pathways. The above results may provide a new perspective on targeting NOD1 signalling for the treatment of PTC, which deserves further investigation.
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http://dx.doi.org/10.1016/j.prp.2022.153880DOI Listing
May 2022

Straightforward synthesis of quinazolin-4(3)-ones visible light-induced condensation cyclization.

RSC Adv 2022 Jan 7;12(3):1494-1498. Epub 2022 Jan 7.

School of Chemistry, Biology and Materials Science, East China University of Technology Nanchang 330013 China

A green, simple and efficient method is developed for the synthesis of quinazolin-4(3)-ones visible light-induced condensation cyclization of 2-aminobenzamides and aldehydes under visible light irradiation. The reaction proceeds using fluorescein as a photocatalyst in the presence of TBHP without the need for a metal catalyst. In addition, this reaction tolerates a broad scope of substrates and could afford a variety of desirable products in good to excellent yields. Thus, the present synthetic method provides a straightforward strategy for the synthesis of quinazolin-4(3)-ones.
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http://dx.doi.org/10.1039/d1ra07944aDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8978923PMC
January 2022

Immobilization of Ag(0) nanoparticles on quaternary ammonium functionalized polyacrylonitrile fiber as a highly active catalyst for 4-nitrophenol reduction.

RSC Adv 2021 Dec 5;12(2):1051-1061. Epub 2022 Jan 5.

Department of Chemistry, College of Pharmacy, North China University of Science and Technology Tang Shan 300072 P. R. China

Ag(0) nanoparticles were immobilized on various pyridine salt, imidazole salt and quaternary ammonium functionalized polyacrylonitrile fibers (PANFs) to prepare Ag(0)-immobilized fiber catalysts. The catalytic activities of these immobilized catalysts for 4-nitrophenol (4-NP) reduction were detected. Among them, the quaternary ammonium fiber with butyl group immobilized Ag(0) nanoparticle catalyst PANF-Ag(0) showed the best catalytic activity, and can effectively catalyze 4-nitrophenol (4-NP) reduction with a high conversation rate of 99.6%. Furthermore, PANF-Ag(0) can be easily recovered, and it was reused 20 times with little decrease in catalytic activity and moderate Ag retention (53.5%). Notably, the cationic groups in the functionalized fibers can stabilize Ag(0) nanoparticles through electrostatic interactions and steric effects, and play an important role in phase transfer catalysis. Accordingly, possible mechanisms for the 4-NP reduction catalyzed by PANF-Ag(0) were proposed.
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http://dx.doi.org/10.1039/d1ra07321dDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8978928PMC
December 2021

Receptor activity-modifying protein 1 regulates mouse skin fibroblast proliferation via the Gαi3-PKA-CREB-YAP axis.

Cell Commun Signal 2022 04 12;20(1):52. Epub 2022 Apr 12.

Department of Plastic Surgery, Shandong Provincial Qianfoshan Hospital, Cheeloo College of Medicine, Shandong University, Jinan, 250012, Shandong, People's Republic of China.

Background: Skin innervation is crucial for normal wound healing. However, the relationship between nerve receptors and wound healing and the intrinsic mechanism remains to be further identified. In this study, we investigated the role of a calcitonin gene-related peptide (CGRP) receptor component, receptor activity-modifying protein 1 (RAMP1), in mouse skin fibroblast (MSF) proliferation.

Methods: In vivo, Western blotting and immunohistochemical (IHC) staining of mouse skin wounds tissue was used to detect changes in RAMP1 expression. In vitro, RAMP1 was overexpressed in MSF cell lines by infection with Tet-On-Flag-RAMP1 lentivirus and doxycycline (DOX) induction. An IncuCyte S3 Live-Cell Analysis System was used to assess and compare the proliferation rate differences between different treatment groups. Total protein and subcellular extraction Western blot analysis, quantitative real-time-polymerase chain reaction (qPCR) analysis, and immunofluorescence (IF) staining analysis were conducted to detect signalling molecule expression and/or distribution. The CUT & RUN assay and dual-luciferase reporter assay were applied to measure protein-DNA interactions.

Results: RAMP1 expression levels were altered during skin wound healing in mice. RAMP1 overexpression promoted MSF proliferation. Mechanistically, total Yes-associated protein (YAP) and nuclear YAP protein expression was increased in RAMP1-overexpressing MSFs. RAMP1 overexpression increased inhibitory guanine nucleotide-binding protein (G protein) α subunit 3 (Gαi3) expression and activated downstream protein kinase A (PKA), and both elevated the expression of cyclic adenosine monophosphate (cAMP) response element-binding protein (CREB) and activated it, promoting the transcription of YAP, elevating the total YAP level and promoting MSF proliferation.

Conclusions: Based on these data, we report, for the first time, that changes in the total RAMP1 levels during wound healing and RAMP1 overexpression alone can promote MSF proliferation via the Gαi3-PKA-CREB-YAP axis, a finding critical for understanding RAMP1 function, suggesting that this pathway is an attractive and accurate nerve target for skin wound treatment. Video Abstract.
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http://dx.doi.org/10.1186/s12964-022-00852-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9004193PMC
April 2022

Separation, Purification, Structural Characterization, and Anticancer Activity of a Novel Exopolysaccharide from sp.

Molecules 2022 Mar 23;27(7). Epub 2022 Mar 23.

Drug Research & Development Center, School of Pharmacy, Wannan Medical College, Wuhu 241002, China.

sp. has a wide range of applications in the food fermentation industry. In this study, a novel exopolysaccharide, labeled MSEPS, was separated from sp. fermentation broth through ethanol precipitation and was purified by ion-exchange chromatography, as well as gel filtration column chromatography. MSEPS was composed mostly of mannose, galactose, fucose, arabinose, and glucose with a molar ratio of 0.466:0.169:0.139:0.126:0.015 and had a molecular weight of 7.78 × 10 Da. The analysis of methylation and nuclear magnetic resonance results indicated that MSEPS mainly consisted of a backbone of →3,6)-α-d-Man-(1→3,6)-β-d-Gal-(1→, with substitution at O-3 of →6)-α-d-Man-(1→ and →6)-β-d-Gal-(1→ by terminal α-l-Ara residues. MTT assays showed that MSEPS was nontoxic in normal cells (HK-2 cells) and inhibited the proliferation of carcinoma cells (SGC-7901 cells). Additionally, morphological analysis and flow cytometry experiments indicated that MSEPS promoted SGC-7901 cell death via apoptosis. Therefore, MSEPS from sp. can be developed as a potential antitumor agent.
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http://dx.doi.org/10.3390/molecules27072071DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9000282PMC
March 2022

Immunoproteasome subunit PSMB8 regulates microglia-mediated neuroinflammation upon manganese exposure by PERK signaling.

Food Chem Toxicol 2022 May 1;163:112951. Epub 2022 Apr 1.

Medical School of Chinese PLA: Chinese PLA General Hospital, 28 Fuxing Road, Haidian District, Beijing, China; Department of Occupational & Environmental Health and the Ministry of Education Key Lab of Hazard Assessment and Control in Special Operational Environment, School of Public Health, Fourth Military Medical University, Xi'an, 710032, China. Electronic address:

Excessive manganese (Mn) exposure gives rise to various neurological disorders, including motor dysfunction and cognitive impairment. Microglia-mediated neuroinflammation plays an essential role in the pathogenesis of Mn neurotoxicity. However, the underlying mechanisms have not been fully clarified. Immunoproteasome is a specialized proteasome. Recent studies have shown that immunoproteasome, especially catalytic subunit PSMB8, is highly associated with various neurological diseases. Whether PSMB8 is involved in Mn-neurotoxicity is still unknown. In this study, in vivo and in vitro models were established, and our data showed that Mn exposure upregulated the expression and activity of PSMB8. Selective inhibition of PSMB8 mitigated neuroinflammation with reduced microglial activation and fewer TNF-α, iNOS, and CCL12 production in Mn-treated mice and BV2 cells. Learning and memory tests and Golgi staining further confirmed that inhibition of PSMB8 alleviated Mn-induced recognition memory impairments and synapse deficits. Besides, we found that blocking of PERK signaling inhibited Mn-induced elevation of PSMB8. And inhibition of PSMB8 reduced the phosphorylation of NF-κB p65. Together, our data demonstrated that PSMB8 played an essential role in microglia-mediated neuroinflammation upon Mn exposure, and the underlying mechanisms may be via PERK/NF-κB pathways. These results provide a novel target for the prevention and treatment of Mn-neurotoxicity.
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http://dx.doi.org/10.1016/j.fct.2022.112951DOI Listing
May 2022

Shade-Tolerant Soybean Reduces Yield Loss by Regulating Its Canopy Structure and Stem Characteristics in the Maize-Soybean Strip Intercropping System.

Front Plant Sci 2022 16;13:848893. Epub 2022 Mar 16.

College of Agronomy, Sichuan Agricultural University, Chengdu, China.

The shading of maize is an important factor, which leads to lodging and yield loss of soybean in the maize-soybean strip intercropping system, especially in areas with low solar radiation. This study was designed to explore how shade-tolerant soybean reduces yield loss by regulating its canopy structure and stem characteristics in the maize-soybean strip intercropping system. The soybean cultivars Tianlong No.1 (TL-1, representative of shade-tolerant plants) and Chuandou-16 (CD-16, representative of shade-intolerant plants) were grown in monocropping and intercropping systems from 2020 to 2021 in Chongzhou, Sichuan, China. Regardless of shade-intolerant or shade-tolerant soybean, the canopy and stem of soybean in strip intercropping were weaker than those of the corresponding monoculture. But compared with shade-intolerant soybean, the shade-tolerant soybean slightly changed its spatial structure of canopy and stem morphology and physiology in maize-soybean strip intercropping system, especially in the later growth stages. On the one hand, the canopy of shade-tolerant soybean showed relatively high transmission coefficient (TC) and relatively low leaf area index (LAI) and mean leaf angle (MLA). On the other hand, the stem of shade-tolerant soybean was obviously stronger than that of shade-intolerant soybean in terms of external morphology, internal structure, and physiological characteristics. Additionally, compared with shade-intolerant soybean, shade-tolerant soybean showed higher APnWP (the average net photosynthetic rate of the whole plant) and seed yield in the strip intercropping. The results showed that shade-tolerant soybean increased light energy capture and photosynthesis in the different canopy levels to promote the morphological and physiological development of the stem and ultimately reduce the yield loss of the strip intercropping system. However, the molecular mechanism of low radiation regulating soybean canopy structure (LAI, TC, and MLA) needs further in-depth research to provide theoretical guidance for cultivating plants with ideal canopy shape that can adapt to changing light environment in intercropping system.
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http://dx.doi.org/10.3389/fpls.2022.848893DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8967133PMC
March 2022

Physiological adjustment of pomegranate pericarp responding to sunburn and its underlying molecular mechanisms.

BMC Plant Biol 2022 Apr 4;22(1):169. Epub 2022 Apr 4.

Key Laboratory of Genetic Improvement and Ecophysiology of Horticultural Crops, Institute of Horticulture, Anhui Academy of Agricultural Sciences, Hefei, 230031, China.

Background: Sunburn is common in pomegranate, and sunburned fruits have poor appearance and low marketability. However, the physiological and metabolic responses to sunburn and their underlying molecular mechanisms in pomegranate fruit are little understood. Fruit of sunburn-sensitive cultivar 'Hongyushizi' was used to carry out physiological parameter detection and widely-targeted metabolomics and transcriptome study.

Results: Malondialdehyde and relative conductivity increased with the severity of sunburn, which indicated increased membrane injury. Meanwhile, the content of antioxidants (total phenols and flavonoids), which reduce and repair membrane damage, increased and were accompanied by increases in total antioxidant capacity. In sunburned fruits compared with controls, 129 metabolites changed (including naringenin, pelargonidin and kaempferol) and 447 differentially expressed genes including CHI (Pgr25966.1), F3'5'H (Pgr26644.1), and CHS (Pgr005566.1) may have contributed to these changes. Transcription factors, such as NAC 5 (Pgr008725.1), MYB 93 (Pgr001791.1), and MYB 111 (Pgr027973.1) may be involved in phenylpropanoid and flavonoid biosynthesis by regulating the CHI, F3'5'H, and CHS etc. CONCLUSIONS: These findings provide insight into the sunburn mechanisms of pomegranate, and also into the genetic improvement of fruit sunburn.
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http://dx.doi.org/10.1186/s12870-022-03534-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8978398PMC
April 2022

Effect of different dosages of sodium butyrate and niacin on growth, faecal microbiota and Vitamin B metabolism in weaned piglets.

J Appl Microbiol 2022 Jun 4;132(6):4466-4475. Epub 2022 Apr 4.

College of Animal Science and Technology, Guangxi University, Nanning, China.

Aims: Our study aimed to evaluate the effects of different dosages of sodium butyrate and niacin on the growth performance, faecal Vitamin B and microbiota in weaned piglets.

Methods And Results: Seventy-two weaned piglets (Duroc × Landrace × Yorkshire, age of 21 days) were randomly assigned to one of six treatments (12 pigs/treatment); the control (CT) group was administered a basal diet. The groups in which concentration ratios of sodium butyrate to niacin were 100: 1, 100: 2, 100: 4, 100: 8 and 100: 16 (BN1, BN2, BN4, BN8 and BN16) were administered a basal diet supplemented with 2000 mg kg sodium butyrate and 20, 40, 80, 160 or 320 mg·kg niacin. After 14-day treatment, the samples were collected. The results showed that feed conversion rate (FCR) was reduced and average daily gain (ADG) was increased in BN2 (p < 0.05). The diarrhoea index of pigs decreased with the low supplement. Additionally, compared with the CT group, other groups significantly increased (p < 0.05) the abundance of Firmicutes (BN4, phylum), Lactobacillaceae (BN8, family), Megasphaera (BN8, genus) and Lactobacillus (BN8, genus). Furthermore, the sodium butyrate and niacin supplementation influence Vitamin B1, Vitamin B2, pyridoxine, niacin, nicotinamide and Vitamin B12 (p < 0.05). Correlation analysis of the association of micro-organisms with Vitamin B indicated that changes of Vitamin B metabolism have a potential correlation with alterations of faecal microbiota in weaned piglets.

Conclusions: The results indicated that adding sodium butyrate and niacin in the diet could promote the performance and improve the faecal microbiota and Vitamin B metabolism in weaned piglets.

Significance And Impact Of The Study: Our study might provide clues to the research of correlations between faecal bacteria and faecal Vitamin B, and these findings will contribute to the direction of future research in weaned piglets.
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http://dx.doi.org/10.1111/jam.15545DOI Listing
June 2022
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