Publications by authors named "Chunyan Huang"

48 Publications

Enhanced GRP78 protein expression via the IRE1α/ASK1/p38 MAPK pathway during AsO-induced endoplasmic reticulum stress in BEAS-2B cells.

Toxicology 2021 Sep 21;462:152962. Epub 2021 Sep 21.

Department of Occupational Medicine and Environmental Toxicology, College of Public Health, Nantong University, Nantong, Jiangsu, 226019, China. Electronic address:

Inorganic arsenic is widely present in the environment. Exposure to moderate to high concentrations of arsenic from drinking water or air can cause various cancers and multisystem dysfunction. Glucose-regulated protein 78 (GRP78) is an endoplasmic reticulum (ER) stress sensor of unfolded protein response (UPR) under stress conditions and it enhances cell survival. The aim of this study is to investigate molecular mechanisms of arsenic-induced GRP78 expression in BEAS-2B cells model. We found that GRP78 protein expression was enhanced, while the level of GRP78 mRNA expression did not change under arsenic trioxide (AsO)-induced ER stress condition in BEAS-2B cells. Cycloheximide, a protein synthesis inhibitor, completely inhibited AsO-induced GRP78 protein expression. GRP78 mRNA expression was inhibited by actinomycin-D (Act-D). However, GRP78 protein expression was upregulated in the presence of Act-D under AsO-induced ER stress condition. These data indicated that the upregulation of GRP78 protein under AsO-induced UPR condition was possibly due to the increased biosynthesis of GRP78 protein. Moreover, both inositol-requiring enzyme 1α (IRE1α) RNase and kinase inhibitor KIRA6 and IRE1α kinase inhibitor APY29 completely inhibited AsO-induced GRP78 protein expression and phosphorylation of JNK, ERK and p38 MAPK. Activation of apoptotic signaling kinase 1 (ASK1) is a downstream effector of IRE1α kinase. ASK1 inhibitor selonsertib and p38 MAPK inhibitor SB203580 partially inhibited AsO-induced GRP78 protein expression, respectively. Our results suggested that AsO enhanced GRP78 protein expression in BEAS-2B cells via IRE1α kinase/ASK1/p38 MAPK signaling pathway. To our knowledge, this is the first report on illuminating the related mechanisms of increased GRP78 protein expression in AsO-induced ER stress condition through a novel IRE1α pathway.
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http://dx.doi.org/10.1016/j.tox.2021.152962DOI Listing
September 2021

Arsenic trioxide induces expression of BCL-2 expression via NF-κB and p38 MAPK signaling pathways in BEAS-2B cells during apoptosis.

Ecotoxicol Environ Saf 2021 Oct 21;222:112531. Epub 2021 Jul 21.

Department of Occupational Medicine and Environmental Toxicology, School of Public Health, Nantong University, Nantong 226019, Jiangsu, China. Electronic address:

Inorganic arsenic compounds are environmental toxicants that are widely distributed in air, water, and food. B-cell lymphoma 2 (BCL-2) is an oncogene having anti-apoptotic function. In this study, we clarify that BCL-2, as a pro-apoptotic factor, participates in AsO-induced apoptosis in BEAS-2B cells. Specifically, AsO stimulated the expression of BCL-2 mRNA and protein in a dose-dependent manner which was highly accumulated in the nucleus of BEAS-2B cell together with chromatin condensation and DNA fragmentation during apoptosis. Mechanistically, the process described above is mediated through the NF-κB and p38 MAPK signaling pathways, which can be abated by corresponding inhibitors, such as BAY11-7082 and SB203580, respectively. Additionally, BAY11-7082, actinomycin D, and cycloheximide have inhibitory effects on AsO-induced expression of BCL-2 mRNA and protein, and restore the cell viability of BEAS-2B cells. Suppression of BCL-2 protein activation by ABT-199 also restored viability of BEAS-2B cell in AsO-induced apoptosis. Furthermore, AsO increased the level of BCL-2 phosphorylation. These results suggest that in BEAS-2B cells, AsO-induced apoptosis is mainly dominated by BCL-2 upregulation, nuclear localization and phosphorylation. The study presented here provides a novel insight into the molecular mechanism of BCL-2-induced apoptosis.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112531DOI Listing
October 2021

Distribution and Clinical Analysis of EpCAM+/Vimentin+ Circulating Tumor Cells in High-Risk Population and Cancer Patients.

Front Oncol 2021 8;11:642971. Epub 2021 Jun 8.

Project Department, Huzhou Lieyuan Medical Laboratory Company Ltd, Huzhou, China.

Circulating Tumor Cells (CTCs) are already present in the peripheral blood of patients with early tumors and even precancerous lesions. The objective of this study was to determine the count of CTCs in peripheral blood from high-risk population(HRP), healthy subjects and patients with Pan-cancer. The CTCs in the peripheral blood from HRP and cancer patients were enriched and identified based on the positive sorting method by epithelial cell adhesion molecular (EpCAM) liposome magnetic bead (Ep-LMB) and Vimentin liposome magnetic bead (Vi-LMB). Simultaneously, further analysis was carried out focusing on the clinical characteristics of patients by collecting the peripheral blood samples from healthy subjects as the parallel control. According to the results, the prepared LMBs had high specificity and stability, resulting in an average (Av) proliferation rate of over 90% for each cell line, and the average capture rate of higher than 80%. In terms of CTCs count detection in clinical blood samples, the average count was 0.9 (Ep: Av=0.6, Vi: Av=0.3), 2.4 (Ep: Av=1.4, Vi: Av=0.8) and 7.3 (Ep: Av=4.0, Vi: Av=3.3) in healthy subjects, HRP and total cancer patients, respectively. Besides, there was no obvious difference in the average count of CTCs among patients with different cancer types. While count of CTCs in the aforementioned cancer patients was statistically different from that in healthy subjects and patients with HRP. The survival time of cancer patients whose number of CTCs is greater than the average is significantly increased. Collectively, the study confirmed that CTCs can achieve early tumor detection and auxiliary diagnosis, and its number is related to the occurrence and development of tumors, and CTCs can be detected in HRP and sub-health population.
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http://dx.doi.org/10.3389/fonc.2021.642971DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8217642PMC
June 2021

Naringin protects H9C2 cardiomyocytes from chemical hypoxia‑induced injury by promoting the autophagic flux via the activation of the HIF‑1α/BNIP3 signaling pathway.

Int J Mol Med 2021 06 28;47(6). Epub 2021 Apr 28.

Department of Cardiology, Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong 524001, P.R. China.

Naringin, a natural bioflavonoid, has been shown to exert protective effects in multiple cardiovascular diseases; however, the protective effects of naringin against hypoxic/ischemia‑induced myocardial are not yet fully understood. Autophagy is a vital factor involved in the pathogenesis of myocardial injury. The aim of the present study was to investigate the protective effects of naringin on H9c2 cells against chemical hypoxia [cobalt chloride (CoCl)]‑induced injury. The role of autophagy and the hypoxia‑inducible factor‑1α (HIF‑1α)/Bcl‑2/BCL2 interacting protein 3 (BNIP3) signaling pathway in the protective effects of naringin were also assessed. The results revealed that naringin pre‑treatment significantly attenuated the CoCl‑induced cytotoxicity and apoptosis, and also decreased caspase‑3 activity, which had been increased by CoCl. In addition, CoCl increased Beclin‑1 expression, enhanced the IL3B‑II/IL3B‑I ratio and increased p62 expression in the H9C2 cells. Treatment with 3‑methyladenine (3‑MA), a selective inhibitor of autophagy, also blocked CoCl‑induced cytotoxicity and apoptosis. Notably, treatment with bafilomycin A1 (Baf A1), an inhibitor of the vacuolar H+ ATPase of lysosomes, resulted in an increase in the upregulation of the LC3B‑II/LC3B‑I ratio, but did not further increase the LC3B‑II/LC3B‑I ratio compared with CoCl treatment. These results suggested that CoCl inhibited the autophagic flux, which resulted in myocardial cell damage. Furthermore, naringin pre‑treatment exacerbated Beclin 1 expression and the increased IL3B‑II/IL3B‑I ratio, and reduced p62 expression in CoCl‑treated H9C2 cells. 3‑MA and Baf A1 both reversed the protective effects of naringin against CoCl‑induced injury, indicating that naringin attenuated CoCl‑induced myocardial cell injury by the increasing autophagic flux. Moreover, naringin treatment resulted in upregulated expression levels of HIF‑1α and BNIP3 in the CoCl‑treated H9C2 cells. The inhibition of the HIF‑1α/BNIP3 signaling pathway using 3‑(5'‑hydroxymethyl‑2'‑furyl)‑1‑benzylindazole (an inhibitor of HIF‑1α) prevented the effects of naringin on the autophagic flux and reversed its protective effects against CoCl‑induced injury. Taken together, these results suggest that naringin protects the H9C2 cells against CoCl‑induced injury by enhancing the autophagic flux via the activation of the HIF‑1α/BNIP3 signaling pathway.
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http://dx.doi.org/10.3892/ijmm.2021.4935DOI Listing
June 2021

Label-free Raman spectroscopy: A potential tool for early diagnosis of diabetic keratopathy.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Jul 22;256:119731. Epub 2021 Mar 22.

Key Laboratory of Opto-Electronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory for Photonics Technology, Fujian Normal University, Fuzhou, China. Electronic address:

Diabetes has become a major public health problem worldwide, and the incidence of diabetes has been increasing progressively. Diabetes is prone to cause various complications, among which diabetic keratopathy (DK) emphasizes the significant impact on the cornea. The current diagnosis of DK lacks biochemical markers that can be used for early and non-invasive screening and detection. In contrast, in this study, Raman spectroscopy, which demonstrates non-destructive, label-free features, especially the unique advantage of providing molecular fingerprint information for target substances, were utilized to interrogate the intrinsic information of the corneal tissues from normal and diabetic mouse models, respectively. Visually, the Raman spectral response derived from the biochemical components and biochemical differences between the two groups were compared. Moreover, multivariate analysis methods such as principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were carried out for advanced statistical analysis. PCA yields a diagnostic results of 57.4% sensitivity, 89.2% specificity, 74.8% accuracy between the diabetic group and control group; Moreover, PLS-DA was employed to enhance the diagnostic ability, showing 76.1% sensitivity, 86.1% specificity, and 87.6% accuracy between the diabetic group and control group. Our proof-of-concept results show the potential of Raman spectroscopy-based techniques to help explore the underlying pathogenesis of DK disease and thus be further expanded for potential applications in the early screening of diabetic diseases.
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http://dx.doi.org/10.1016/j.saa.2021.119731DOI Listing
July 2021

Change in mortality rates of respiratory disease during the COVID-19 pandemic.

Expert Rev Respir Med 2021 08 31;15(8):1083-1088. Epub 2021 Mar 31.

Department of Chronic Disease Prevention and Control, Suzhou Center for Disease Control and Prevention, Suzhou, Jiangsu, China.

Objective: This study explored the change in mortality rates of respiratory disease during the corona virus disease 2019 (COVID-19) pandemic.

Methods: Death data of registered residents of Suzhou from 2014 to 2020 were collected and the weekly mortality rates due to respiratory disease and all deaths were analyzed. The differences in mortality rates during the pandemic and the same period in previous years were compared.

Results: Before the pandemic, the crude mortality rate (CMR) and standardized mortality rate (SMR) of Suzhou residents including respiratory disease, were not much different from those in previous years. During the emergency period, the CMR of Suzhou residents was 180.2/100,000 and the SMR was 85.5/100,000, decreasing by 9.1% and 14.6%, respectively; the CMR of respiratory disease was 16.4/100,000 and the SMR was 6.8/100,000, down 41.4% and 44.9%, respectively. Regardless of the mortality rates of all deaths or respiratory disease, the rates were higher in males than in females, although males had aslightly greater decrease in all deaths during the emergency period compared with females, and the opposite was true for respiratory disease.

Conclusion: During the pandemic, the death rate of residents decreased, especially that due to respiratory disease.
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http://dx.doi.org/10.1080/17476348.2021.1908891DOI Listing
August 2021

LncRNA H19 regulates macrophage polarization and promotes Freund's complete adjuvant-induced arthritis by upregulating KDM6A.

Int Immunopharmacol 2021 Apr 1;93:107402. Epub 2021 Feb 1.

Department of Immunology, Mudanjiang Medical University, Mudanjiang, Heilongjiang 157011, China. Electronic address:

Aberrant expression of long non-coding RNA (lncRNA) H19 is tightly linked to multiple steps of tumorigenesis via the modulation of cell proliferation and apoptosis; however, the pathological significance and regulatory mechanisms of lncRNA H19 in macrophages remain obscure. To investigate whether lncRNA H19 modulates macrophage activation in rheumatoid arthritis (RA), lncRNA H19 levels in PMA-induced PBMC from patients with RA and healthy volunteers were assessed. In addition, the distribution of macrophage subsets, macrophage phenotypic characteristics, and pro-inflammatory gene expression were examined in lncRNA H19 smart silencer- or pcDNA 3.1- H19-transfected macrophages and AAV8-mediated H19 overexpression in a Freund' s complete adjuvant-induced arthritis mouse model. The level of lncRNA H19 was higher in RA patients than in healthy volunteers. Silencing of lncRNA H19 altered lipopolysaccharide plus interferon-induced M1 macrophage polarization and decreased IL-6, CD80, CCL8, and CXCL10 expression in macrophages of RA patients. LncRNA H19 overexpression markedly induced IL-6, CD80, HLA-DR, KDM6A, STAT1, IRF5, CCL8, CXCL9, CXCL10, and CXCL11 expression in macrophages and promoted macrophage migration. AAV8-mediated H19 overexpression aggravated arthritis in mice by promoting M1 macrophage polarization along with iNOS, IL-6, CCL8, CXCL9, CXCL10, CXCL11, MMP3, MMP13 and COX-2 expression in mononuclear cells isolated from the swollen ankle. GSK-J4, an inhibitor of KDM6A, suppressed the activity of lncRNA H19 in macrophages and ameliorated lncRNA H19-aggravated arthritis. In summary, the current study demonstrated that lncRNA H19 is upregulated in RA patients and arthritic mice. LncRNA H19 promotes M1 macrophage polarization and aggravates arthritis by upregulating KDM6A expression.
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http://dx.doi.org/10.1016/j.intimp.2021.107402DOI Listing
April 2021

Gut microbiota affects the efficacy of Danggui Buxue Tang by affecting plasma concentration of active ingredients.

J Ethnopharmacol 2021 Apr 16;270:113835. Epub 2021 Jan 16.

School of Bioscience and Biopharmaceutics, Guangdong Province Key Laboratory for Biotechnology Drug Candidates, Guangdong Pharmaceutical University, 510006, Guangzhou, Guangdong, China. Electronic address:

Ethnopharmacological Relevance: Danggui Buxue Tang (DBT) is a traditional Chinese medicine, which has the function of supporting Qi and enriching blood. Antibiotics can cause Gut microbiota disorder and affect efficacy of DBT.

Aim Of The Study: Explore the manner in which Gut microbiota affects the efficacy of Danggui Buxue Tang.

Materials And Methods: In this study, antibiotics were used to destroy gut microbiota. The changes of DBT efficacy were detected to verify the effect of gut microbiota on DBT efficacy. The changes of gut microbiota was detected using 16S rRNA sequencing, and UPLC-MS/MS was used to analyze the plasma concentration of active ingredients. Correlation analysis was used to establish the relationship between gut microbiota, blood components and drug efficacy, and to explore the role of gut microbiota in the efficacy of DBT.

Results: The results showed that the efficacy in the DBT group was significantly improved compared with the control group (p<0.05). Compared with DBT group, the efficacy in antibiotic DBT treatment (ABXDBT) group was significantly reduced, 194 plasma metabolites and 18 DBT blood components were significantly altered in ABXDBT group, and 11 DBT blood components such as caffeic acid and formononetin were significantly decreased. Correlation analysis showed that 6 DBT blood components were related with the decrease of efficacy. Network pharmacology analysis showed that the above 6 DBT blood components participated in the hematopoietic regulation through PI3K-Akt and HIF-1 signaling pathways. Correlation analysis showed that Bacteroides and other intestinal bacteria were related to the absorption of DBT active ingredients. The drug metabolic pathway of gut microbiota was significantly decreased after antibiotic treatment (p = 0.033).

Conclusions: Gut microbiota such as Bacteroides affects the efficacy of DBT by affecting the metabolism and absorption of DBT active ingredients such as caffeic acid and formononetin.
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http://dx.doi.org/10.1016/j.jep.2021.113835DOI Listing
April 2021

Effect of PAC on the Behavior of Dynamic Membrane Bioreactor Filtration Layer Based on the Analysis of Mixed Liquid Properties and Model Fitting.

Membranes (Basel) 2020 Dec 14;10(12). Epub 2020 Dec 14.

School of Space and Environment, Beihang University, Beijing 100191, China.

Recently, dynamic membrane bioreactor (DMBR) has gradually gained the interest of researchers for the development of membrane technology. In this paper, we set up parallel experiments to investigate the effect of powder activated carbon (PAC) on organic matter removal, transmembrane pressure, and filter cake layer characterization to make an overall performance assessment of DMBR. The results showed that DMBR has a good removal effect on organic matter removal, and with a chemical oxygen demand removal rate over 85%. Protein was found to be the main membrane fouling substance. Due to the electric double-layer effect, membrane fouling tended to be alleviated when the PN/PS value was low. Using a filtration model under constant current conditions, the filtration process through the cake layer was observed to be consistent with cake-intermediate model.
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http://dx.doi.org/10.3390/membranes10120420DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7765143PMC
December 2020

Can Next-generation Sequencing Replace Fecal Immunochemical Tests or CT in the Screening of Colorectal Cancer and Advanced Adenoma?

J Coll Physicians Surg Pak 2020 09;30(9):940-945

Department of Gastroenterology, Renmin Hospital of Wuhan University, China.

Objective: To explore the feasibility of next-generation sequencing (NGS) for the screening of colorectal cancer (CRC) and advanced adenoma (AA).

Study Design: Observational study. Place and Duration of the Study: Renmin Hospital of Wuhan University, Wuhan, China, from June 2019 to February 2020.

Methodology: Patients who met inclusion and exclusion criteria were divided into three groups: CRC, AA, and control group. Then, the gene methylation status in the blood in the CRC, AA, and control group was analysed by NGS, and the CRC screening risk assessment model was used for comprehensive analysis. Afterwards, the methylated haplotype index (PHF Index) was calculated, and the screening results of the patients were classified as positive or negative, according to the score. The clinicopathological results were used as the gold standard, and the screening results for NGS were compared with the computed tomography (CT) and fecal immunochemical test (FIT) to text its feasibility.

Results: NGS has a certain detection ability for CRC, with a sensitivity of 57.1% (8/14). This was higher than that of FIT and CT, and the combined positive rate of these three methods could reach 92.3% (12/13). The sensitivity of detection of AA could reach up to 75.0% (6/8) after combining with FIT and CT. The positive rate of the NGS test for postoperative CRC was 23.1% (3/13), which was significantly lower than preoperative CRC. The sensitivity of CT for preoperative CRC detection was only 45.5% (5/11), but the specificity could reach up to 98.2% (55/56), which was higher than NGS (71/78, 91.0%) and FIT (27/33, 81.8%).

Conclusion: Although NGS cannot replace FIT and CT at present, this provides a new effective and auxiliary detection method for people who are unsuitable or unwilling to receive colonoscopy. Key Words: Colorectal neoplasms, Adenoma, High-throughput nucleotide sequencing, Mass screening, Fecal immunochemical tests, Computed tomography.
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http://dx.doi.org/10.29271/jcpsp.2020.09.940DOI Listing
September 2020

Danggui Buxue Tang restores antibiotic-induced metabolic disorders by remodeling the gut microbiota.

J Ethnopharmacol 2020 Sep 11;259:112953. Epub 2020 May 11.

School of Bioscience and Biopharmaceutics, Guangdong Province Key Laboratory for Biotechnology Drug Candidates, Guangdong Pharmaceutical University, 510006 Guangzhou, Guangdong, China. Electronic address:

Ethnopharmacological Relevance: Danggui Buxue Tang (DBT) has been used to promote hematopoiesis and relieve myelosuppression in China. Antibiotics can cause myelosuppression through gut microbiota disorders.

Aim Of The Study: This study aims to explore the way of DBT to alleviate the metabolic disorder caused by antibiotics.

Materials And Methods: In this study, 16S rRNA sequencing was used to detect the change of gut microbiota, metabolomics to analyze the change of metabolites. Correlation analysis was used to establishment the correlation between gut microbiota and metabolites. PICRUST 2 was used to predict the function of gut microbiota.

Results: Results showed that eighty-two genera of gut microbiota were affected by antibiotic, while twelve were significantly restored after DBT. Seventy-four potential metabolites were significantly different from the antibiotics and DBT. We found significant recovery by the Bacteroides and Rikenellaceae RC9 after DBT. The metabolic pathways influenced by the antibiotic treatment included primary and secondary bile biosynthesis, etc. The metabolic pathways that could be restored after DBT included the primary and secondary bile acid biosynthesis pathway, etc. Through correlation analysis, we found a correlation between the Bacteroides, Rikenellaceae_RC9_gut_group and other potential differential metabolisms such as those of taurodeoxycholic acid, N-phenylacetyl glycine, etc. The functional prediction showed that the biosynthesis of primary bile acid, secondary bile acid was significantly affected.

Conclusions: DBT can restore the gut and reverse the metabolic disorder caused by antibiotics through Bacteroides, and it provides a new medical idea regarding the gut microbiota balance.
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http://dx.doi.org/10.1016/j.jep.2020.112953DOI Listing
September 2020

Metformin Attenuates Ischemia-reperfusion Injury of Fatty Liver in Rats Through Inhibition of the TLR4/NF-κB Axis

Balkan Med J 2020 06 9;37(4):196-202. Epub 2020 Apr 9.

Department of Hospitality, Affiliated Hospital of Jiujiang University, Jiangxi, China

Background: Donor organs for liver transplantation may often have fatty liver disease, which confers a higher sensitivity to ischemia/reperfusion injury. At present, there is no effective treatment for the condition. Evidence has suggested that metformin, the first-line medication for diabetes, has protective effects against many disorders. However, the potential role of metformin in ischemia/reperfusion injury in fatty liver disease remains unclear.

Aims: To examine the effect of metformin treatment during ischemia/reperfusion injury in fatty liver and determine the possible mechanisms.

Study Design: Animal experimentation.

Methods: Sprague-Dawley male rats were fed a high-fat diet (520 kcal/100 g) for 14 weeks and then were subjected to the orthotopic autologous liver transplantation model. Sections of liver tissue were stained with hematoxylin and eosin to visualize the damage. Blood and liver samples were used to analyze the related proteins and components involved in the inflammatory signaling pathway.

Results: We found that metformin significantly ameliorated the ischemia/reperfusion injury of the fatty liver through a reduction in alanine aminotransferase/aspartate aminotransferase concentrations in the serum and a decrease in dead cells, as shown by the terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling assay (p<0.05). In addition, metformin significantly attenuated interleukin (IL)-6, IL-1β, and tumor necrosis factor-α production and increased the expression of active caspase-3 and Bax in the liver (p<0.05). Mechanistically, metformin suppressed the activation of toll-like receptor 4 (TLR4)/NF-κB signaling (p<0.05), resulting in a decreased inflammatory response and apoptosis.

Conclusion: Our findings demonstrated that metformin attenuated ischemia/reperfusion injury in fatty liver disease via the TLR4/NF-κB axis, suggesting that metformin could have potential therapeutic applications in ischemia/reperfusion injury associated with liver transplantation.
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http://dx.doi.org/10.4274/balkanmedj.galenos.2020.2019.9.31DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7285667PMC
June 2020

RNA 6-methyladenosine: a promising molecular target in metabolic diseases.

Cell Biosci 2020 21;10:19. Epub 2020 Feb 21.

4Hangzhou Xixi Hospital Affiliated to Zhejiang Chinese Medical University, Hangzhou, 310023 Zhejiang China.

6-methyladenosine is a prevalent and abundant transcriptome modification, and its methylation regulates the various aspects of RNAs, including transcription, translation, processing and metabolism. The methylation of 6-methyladenosine is highly associated with numerous cellular processes, which plays important roles in the development of physiological process and diseases. The high prevalence of metabolic diseases poses a serious threat to human health, but its pathological mechanisms remain poorly understood. Recent studies have reported that the progression of metabolic diseases is closely related to the expression of RNA 6-methyladenosine modification. In this review, we aim to summarize the biological and clinical significance of RNA 6-methyladenosine modification in metabolic diseases, including obesity, type 2 diabetes, non-alcoholic fatty liver disease, hypertension, cardiovascular diseases, osteoporosis and immune-related metabolic diseases.
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http://dx.doi.org/10.1186/s13578-020-00385-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7035649PMC
February 2020

A low-cost, accurate method for detecting reticulocytes at different maturation stages based on changes in the mitochondrial membrane potential.

J Pharmacol Toxicol Methods 2020 Jan - Feb;101:106664. Epub 2019 Dec 16.

School of Bioscience and Biopharmaceutics, Guangdong Province Key Laboratory for Biotechnology Drug Candidates, Guangdong Pharmaceutical University, 510006 Guangzhou, Guangdong, China. Electronic address:

In the clinical setting, reticulocytes are used as an index for the hematopoietic function of the bone marrow. Different maturation stages of reticulocytes are early markers for bone marrow hematopoietic stem cell transplantation and bone marrow regeneration after chemotherapy. Therefore, we aimed to establish a method for detecting the different reticulocyte maturation stages. Based on the decreases in mitochondrial membrane potential during reticulocyte maturation, we used MitoTracker Green (MTG)/tetramethylrhodamine, ethylester (TMRE) to identify the different reticulocyte maturation stages and used Hoechst33342 to exclude nucleated cells. The results show that this method was universal and could be applied to detect the proportions of reticulocytes in different samples. Their proportion in normal peripheral blood, a blood deficiency model, bone marrow, and spleen were (6 ± 2)%, (38 ± 4)%, (14 ± 4)%, and (3 ± 1)%, respectively. The results obtained using this method were similar to those obtained using the manual counting method (methylene blue); the correlation was good (R = 0.817; p < .01) and the coefficient of variation was lower for the method established. Moreover, reticulocytes in peripheral blood could be further divided into three distinct maturation stages: R1 (MTG/TMRE), R2 (MTG/TMRE), and R3 (MTG/TMRE). Reticulocytes in the bone marrow and spleen could be further divided into four distinct maturation stages: R1 (MTG/TMRE), R2-1 (MTG/TMRE/FS), R2-2 (MTG/TMRE/FS), and R3 (MTG/TMRE). Based on changes in mitochondrial membrane potential, MTG/TMRE/Hoechst33342 staining could be used to detect reticulocytes in different samples and at different maturation stages with low cost and high accuracy.
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http://dx.doi.org/10.1016/j.vascn.2019.106664DOI Listing
November 2020

Impacts of Clinical Pharmacist Intervention on the Secondary Prevention of Coronary Heart Disease: A Randomized Controlled Clinical Study.

Front Pharmacol 2019 8;10:1112. Epub 2019 Oct 8.

Department of Pharmacy, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Coronary heart disease (CHD) is one of the leading causes of morbidity and mortality worldwide, and more efforts should be made to reduce the risk of cardiovascular events. This study aimed to investigate the impact of clinical pharmacist intervention on the prognosis of acute coronary syndrome (ACS) in Chinese patients with CHD. Two hundred and forty patients who had ACS were recruited. Participants were randomly assigned to the intervention group ( = 120) or the control group ( = 120). The intervention group received a medication assessment and education by the clinical pharmacist at discharge and telephone follow-ups at 1 week and 1 and 3 months after discharge. The control group received usual care. The primary outcomes of this study were the proportion of patients who had major adverse cardiovascular events (MACEs), including mortality, nonfatal myocardial infarction (MI), stroke, and unplanned cardiac-related rehospitalizations within 6 and 12 months after hospital discharge. Secondary outcome was self-reported medication adherence to evidence-based medications for CHD (antiplatelets, statins, β-blockers, and angiotensin-converting enzyme inhibitors or angiotensin receptor blockers). Of 240 enrolled patients, 238 (98.3%) completed 6-month follow-up, and 235 (97.9%) completed 12-month follow-up. There were no significant differences between intervention and control groups in the percentages of patients who incurred MACEs within the 6-month follow-up (3.3% vs 7.6%, respectively, = 0.145) or 12-month follow-up (10.9% vs 12.1%, respectively, = 0.783). Significant improvements were found in the prescribing rates of β-blockers and all four classes of medications at discharge in the intervention group compared with the control group ( = 0.001 and = 0.009, respectively). There was no significant difference between the intervention and control groups in the use of all four classes of medications at the 6-month follow-up (48.3% vs 45.8%, respectively, = 0.691) and 12-month follow-up (47.9% vs 46.6%, respectively, = 0.836). The use of β-blockers was nonsignificantly higher in the intervention group than in the control group at the 6-month follow-up (74.2% vs. 64.4%, = 0.103) and 12-month follow-up (74.8% vs 63.8%, = 0.068). Clinical pharmacist intervention had no significant effects on reduction in cardiovascular events among patients with CHD. Further studies with larger sample sizes and longer time frames for both intervention and follow-up are needed to validate the role of the clinical pharmacist in the morbidity and mortality of CHD. chictr.org.cn, identifier ChiCTR-IOR-16007716.
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http://dx.doi.org/10.3389/fphar.2019.01112DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6791923PMC
October 2019

Association between environmental tobacco smoke exposure and risk of type 2 diabetes mellitus in Chinese female never smokers: A population-based cohort study.

J Diabetes 2020 Apr 21;12(4):339-346. Epub 2019 Nov 21.

Department of Chronic Disease Prevention and Control, Suzhou Center for Disease Control and Prevention, Suzhou, China.

Background: Evidence for the association between environmental tobacco smoke (ETS) exposure and the risk of type 2 diabetes mellitus (T2DM) is limited. The aim of this study was to investigate this association in female never smokers.

Methods: We analyzed 28 177 female participants of the China Kadoorie Biobank (CKB) in the Suzhou area, who were never smokers and had no diabetes. ETS exposure was defined as being exposed to other people's tobacco smoke either at home, workplace, or in public places at least 1 d/wk. Cox proportional hazard regression models were used to assess the association between ETS exposure and incident T2DM according to the frequency and duration of ETS exposure.

Results: A total of 774 incident cases of T2DM were identified during a median 7.3-year follow-up. Compared with no ETS exposure, hazard ratios (95% CIs) for all ETS exposure, daily, and ≥14 h/wk ETS exposure were 1.17 (1.00-1.37), 1.23 (1.04-1.46), and 1.25 (1.03-1.53), respectively. Moreover, a positive dose-response relationship was observed between ETS exposure level and T2DM (all P <.05 for trend).

Conclusions: This prospective study suggests that ETS exposure increases the risk of T2DM incidence with dose-response relationship in female never smokers. Thus, reducing ETS exposure may help decrease the burden of T2DM in Chinese females.
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http://dx.doi.org/10.1111/1753-0407.13001DOI Listing
April 2020

Characterization, Antioxidant, Anti-Aging and Organ Protective Effects of Sulfated Polysaccharides from .

Molecules 2019 Sep 28;24(19). Epub 2019 Sep 28.

College of Life Science, Shangdong Agricultural University, Taian 271018, China.

As an irreversible and complex degenerative physiological process, the treatment for aging seems strategically necessary, and polysaccharides play important roles against aging owing to their abundant bioactivities. In this paper, the antioxidant and anti-aging activities of polysaccharides (FPS) and its sulfated FPS (SFPS) on d-galactose-induced aging mice were investigated. The in vitro antioxidant activities demonstrated that SFPS had strong reducing power and superior scavenging effects on 2, 2-diphenylpicrylhydrazyl (DPPH), hydroxyl radicals and the chelating activities of Fe. The in vivo animal experiments manifested that the SFPS showed superior antioxidant and protective abilities against the d-galactose-induced aging by increasing the antioxidant enzyme activities, decreasing lipid peroxidation, improving the inflammatory response and ameliorating the anile condition of mice. Furthermore, the structural analysis of SFPS was investigated through FT-IR, NMR, and HPLC analysis, and the results indicated that SFPS was a homogeneous heteropolysaccharide with a weight-average molecular weight of 2.81 × 10 Da. Furthermore, SFPS has also changed in characteristic functional groups and monosaccharide composition compared to FPS. These results suggested that sulfated modification could enhance the anti-oxidation, anti-aging and protective activities of polysaccharides, which may provide references for the development of functional foods and natural medicines.
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http://dx.doi.org/10.3390/molecules24193517DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6803911PMC
September 2019

Blood utilization in five Chinese hospitals shows low hemoglobin thresholds in medical patients.

Transfusion 2019 09 31;59(9):2820-2826. Epub 2019 May 31.

Division of Transfusion Medicine, Stanford University School of Medicine, Palo Alto, California.

Background: The number of red blood cell units transfused per capita in China is lower than in western countries and the reason(s) for the difference is unknown.

Study Design And Methods: We randomly chose 5050 transfused patients from five Chinese hospitals. We compared transfused cases to nontransfused controls matched for the same underlying diagnosis. We assessed the pretransfusion hemoglobin (Hb) trigger and other clinical characteristics associated with transfusion. After stratifying by underlying disease, we compared pretransfusion Hb level in Chinese hospitals to 12 US hospitals.

Results: In 5050 patients who received transfusion, the pretransfusion Hb levels were lower in medical (6.3 g/dL) compared to surgical patients receiving transfusion postoperatively (8.1 g/dL). In patients with nonsurgical diagnoses, the pretransfusion Hb was much lower than that in the United States; the difference in mean Hb level varied by underlying diagnosis from 0.4 to 1.8 g/dL. In case-control analysis of cases (n = 1356) compared to controls (n = 1201), the pretransfusion Hb showed the strongest association with transfusion. Compared to 10 g/dL, the odds ratio (95% confidence interval) for pretransfusion Hb of 7 to 7.9 g/dL was 37.7 (24.8-57.4).

Conclusion: Transfusion triggers in five Chinese hospitals appear comparable to those in the United States for surgical patients; however, medical patients have lower pretransfusion Hb levels (approx. 6 g/dL). Of the factors assessed, the pretransfusion Hb was most strongly associated with transfusion. The clinical impact of lower transfusion thresholds used in China is unknown.
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http://dx.doi.org/10.1111/trf.15385DOI Listing
September 2019

Antioxidant and Hypoglycemic Effects of Acidic-Extractable Polysaccharides from on Type 2 Diabetes Mice.

Oxid Med Cell Longev 2018 25;2018:9150807. Epub 2018 Nov 25.

College of Life Science, Shandong Agricultural University, Tai'an 271018, China.

The present work was performed to evaluate the effect of acidic-extractable polysaccharides (AE-PS) from fruit bodies of on type 2 diabetes mellitus (T2DM) and its structural characteristics. The T2DM mice induced by high-fat diet (HFD) and streptozotocin (STZ) were administered with 100 and 400 mg/kg AE-PS for 4 weeks. Our work proved that AE-PS decreased the levels of serum lipid, lipid peroxidation, and blood glucose; improved glucose and insulin resistance; enhanced antioxidant enzyme activities; and attenuated the injuries of the liver, kidney, and pancreas in T2DM mice. These results might offer references for the exploitation of AE-PS as functional foods or natural drug source for preventing and treating HFD- and STZ-induced T2DM. Moreover, gas chromatography (GC) results revealed that AE-PS was heterogeneous and composed of fucose, ribose, arabinose, xylose, mannose, galactose, and glucose with mass percentages of 1.23%, 0.57%, 0.29%, 2.12%, 2.73%, 4.66%, and 88.4%, respectively. Fourier-transform infrared (FTIR) and nuclear magnetic resonance (NMR) analysis indicated that AE-PS was a pyran-type polysaccharide with - and -configurations.
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http://dx.doi.org/10.1155/2018/9150807DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6286747PMC
February 2019

Obstructive jaundice caused by myeloid sarcoma in duodenal ampulla.

Dig Liver Dis 2019 02 5;51(2):321. Epub 2018 Sep 5.

First Affiliated Hospital of Nanchang University, General Surgery Center, China.

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http://dx.doi.org/10.1016/j.dld.2018.08.004DOI Listing
February 2019

Enhanced fluoride adsorption of aluminum humate and its resistance on fluoride accumulation in tea leaves.

Environ Technol 2020 Jan 27;41(3):329-338. Epub 2018 Jul 27.

College of Chemistry and Materials Science, Sichuan Normal University, Chengdu, People's Republic of China.

Tea trees () can take in fluorine from soil and the content of fluorine in tea increases with maturity, leading to high content of fluoride in tea leaves and tea products. Long-term consumption of high fluoride tea products could result in chronic fluoride intoxication. Confining the fluoride in the earth with absorbents to reduce the fluoride accumulation of the tea trees during the growth period which could radically control the fluoride level in tea product. Humic acid (HA), a kind of organic matter in the earth was used as raw material to prepare adsorbent aluminum humate (HAA) by aluminum modification. The HAA absorbent presented excellent absorption performance to the fluoride in a wide pH range (4-10), and the maximum adsorptive capacity can reach to 62.5 mg/g. The absorption isotherm demonstrated the adsorption of fluoride was the monomolecular adsorption and the absorption was in accordance with the pseudo-second order kinetic equation. Fluoride content in real soil solution decreased significantly by 53.03% by using the HAA absorbent. The utilization of HAA adsorbent in the culture and field plots experiments also obviously adsorb the soluble fluoride in solution and soil, which could significantly suppress the fluoride accumulation in tea leaves. In September, the fluoride accumulation in tea leaves has been reduced 74.29% in the field plots experiments.
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http://dx.doi.org/10.1080/09593330.2018.1498135DOI Listing
January 2020

The role of kinesin KIF18A in the invasion and metastasis of hepatocellular carcinoma.

World J Surg Oncol 2018 Feb 21;16(1):36. Epub 2018 Feb 21.

Laboratory of Hepatobiliary and Pancreatic Surgery, Affiliated Hospital of Guilin Medical University, Guilin, 541001, Guangxi, People's Republic of China.

Background: KIF18A is associated with a variety of tumours; however, the specific mechanism of action of KIF18A in hepatocellular carcinoma (HCC) remains unclear. In this study, in vitro and in vivo experiments were performed with the aim of exploring the potential function and molecular mechanism of kinesin KIF18A in the occurrence and development of HCC.

Methods: We detected the expression of KIF18A in tumour and adjacent tissues as well as cell proliferation, cell invasion and migration in hepatoma cells after silencing KIF18A. KIF18A-silenced hepatoma cells were subcutaneously injected into nude mice to verify the tumorigenicity of KIF18A. We also detected the expression of signal pathway-related proteins in hepatoma cells after KIF18A knockdown with the aim of exploring the association between KIF18A and related signalling pathways.

Results: The level of KIF18A protein was higher in liver cancer tissues than adjacent tissues. After silencing KIF18A in SMMC-7721 and HepG2 cells, cell growth was obviously inhibited; the migration and invasion abilities were significantly decreased and the in vivo tumour weight was decreased compared to the control group (0.201 ± 0.088 g vs 0.476 ± 0.126 g, p = 0.009). The expression of cell cycle-related protein (cyclin B1), invasion and metastasis-related proteins (MMP-7 and MMP-9) and Akt-related proteins in hepatoma cells was also decreased after knocking down KIF18A.

Conclusions: KIF18A may promote proliferation, invasion and metastasis of HCC cells by promoting the cell cycle signalling pathway as well as the Akt and MMP-7/MMP-9-related signalling pathways and may serve as a new target for the diagnosis and treatment of HCC.
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http://dx.doi.org/10.1186/s12957-018-1342-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5822562PMC
February 2018

Changes in Life Expectancy From 2006 to 2015 in Suzhou, East China: Contributions of Age- and Cause-Specific Mortality.

Asia Pac J Public Health 2018 01;30(1):75-84

2 Nantong University, Nantong, Jiangsu, China.

This study was designed to estimate the contribution of age- and disease-specific mortality, particularly that attributable to chronic noncommunicable diseases and at-birth life expectancy (LE) in Suzhou, East China, between 2006 and 2015. In total, data on 427 290 deaths were analyzed. From 2006 to 2015, the at-birth LE increased from 78.92 years to 82.87 years in Suzhou. A decrease in all-cause age-specific death rates contributed to an increase of 1.98 years. The decreased death rates attributable to noncommunicable diseases including cerebrovascular diseases, cancer, heart disease, and respiratory diseases resulted in an increased LE of 1.37 years, which was particularly pronounced among people aged 65 years and older. However, the prevalence of cancer in those aged 45 to 74 years, particularly gastric, liver, and esophageal cancers, contributed the most to the increase in LE. These data may be useful for public health communications.
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http://dx.doi.org/10.1177/1010539517752429DOI Listing
January 2018

Influence of genetic polymorphisms of IL23R, STAT3, IL12B, and STAT4 on the risk of aplastic anemia and the effect of immunosuppressive therapy.

Ann Hematol 2018 Apr 12;97(4):685-695. Epub 2018 Jan 12.

Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, 610041, People's Republic of China.

Studies have suggested that IL-23/STAT3 and IL-12/STAT4 signaling pathways associate with aplastic anemia (AA) occurrence. Polymorphisms in pathway-related genes may contribute to AA risk. In the current study, we investigated the association between polymorphisms in genes of IL23R, STAT3, IL12B, and STAT4 and occurrence, severity, and immunosuppressive outcome of AA in the Han population in southwest China. In the current 164 AA cases and 211 controls study, we found T allele and TT genotype of rs7574865 were more frequent in the cases than that in the controls. In the additive model, individual carrying rs7574865 T allele demonstrated a 37% (OR (95% CI) = 1.37 (1.02-1.85), Pper = 0.036) increased AA risk. In the recessive model, carrier with rs7574865 TT genotype showed a 2.08-fold increased AA risk (OR (95% CI) = 2.08 (1.14-3.70), Pper = 0.017). Additionally, we showed that G allele and GG genotype of rs11209032 were more frequent in the 88 non-severe AA cases than that in the 76 severe AA ones. Our study also found G allele and GG genotype of rs11209032, and GG-genotype of rs744166 associated with the immunosuppressive therapy outcome in AA patients. Current study results support that functional STAT4 (rs7574865), IL23R (rs11209032), and STAT3 (rs744166) variants may associate with occurrence, severity, and immunosuppressive outcome of AA in the Han population in southwest China.
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http://dx.doi.org/10.1007/s00277-018-3227-7DOI Listing
April 2018

Determination of Sulfonamides in Chicken Muscle by Pulsed Direct Current Electrospray Ionization Tandem Mass Spectrometry.

J Agric Food Chem 2017 Sep 11;65(37):8256-8263. Epub 2017 Sep 11.

Chengdu Institute of Biology, Chinese Academy of Sciences , No. 93 South Keyuan Road, Gaoxin Distinct, Chengdu 610041, P. R. China.

A simple and rapid approach for the simultaneous detection of trace amounts of six sulfonamides in chicken muscle was developed using pulsed direct current electrospray ionization tandem mass spectrometry (pulsed-dc ESI-MS/MS). The pretreatment of chicken muscle samples consisted of two steps: acetonitrile extraction and n-hexane delipidation. Sulfonamides do not need to be derivatized or chromatographed prior to pulsed-dc ESI-MS/MS. The factors affecting the performance of pulsed-dc ESI-MS/MS were studied. Under optimum conditions, the quantitative performance of pulsed-dc ESI-MS/MS was validated according to European Union Decision 2002/657/EC, and the sensitivity of pulsed-dc ESI-MS/MS was 3 times higher than that of ultrahigh-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The limits of detection obtained by pulsed-dc ESI-MS/MS were in the range of 0.07-0.11 μg/kg. The proposed method was simple, rapid, and sensitive, and was successfully used for quantitation and rapid screening of sulfonamides in real chicken muscle samples.
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http://dx.doi.org/10.1021/acs.jafc.7b03803DOI Listing
September 2017

Investigation on left ventricular multi-directional deformation in patients of hypertension with different LVEF.

Cardiovasc Ultrasound 2017 Jun 12;15(1):14. Epub 2017 Jun 12.

Department of Ultrasound, the First Affiliated Hospital of Fujian Medical University, Fuzhou, 350005, China.

Background: This study is aimed at investigating myocardial multi-directional systolic deformation in hypertensive with different left ventricular ejection fraction (LVEF), and exploring its contribution to LVEF.

Methods: One hundred and twenty-three patients with primary hypertension (HT) were divided into group A (LVEF ≥ 55%), group B (45% ≤ LVEF < 50%, or 50% ≤ LVEF < 55% + LVEDVI ≥ 97 ml/m), and group C (LVEF < 45%). Two-dimensional strain echocardiography (2DSE) including LV longitudinal strain (SL), radial strain (SR) and circumferential strain (SC) were measured.

Results: SL decreased gradually from group A, B to C (all p < 0.05) while SR and SC were reduced only in group B and C (all p < 0.05). All strain measurements correlated to LVEF, with the strongest correlation in SC (r = -0.82, p < 0.01) and the second in SL (r = -0.76). The diastolic E/e increased from group A, B to C.

Conclusions: Left ventricular multi-directional deformation correlated well to LVEF in hypertension and particularly SC, indicating that it was SC, not SL or SR, that makes the prominent contribution to left ventricular pump function.
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http://dx.doi.org/10.1186/s12947-017-0106-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5469145PMC
June 2017

Identification of a novel FUT1 allele with two mutations in a Chinese para-Bombay individual.

Transfusion 2017 01 27;57(1):93-96. Epub 2016 Dec 27.

Department of Blood Immunology, Institute of Blood Transfusion, Chinese Academy of Medical Sciences, Chengdu, Sichuan, China.

Background: The para-Bombay phenotype often results from a silenced β-D-galactoside 2-α-fucosyltransferase 1 (FUT1) gene (h/h) but an active FUT2 (Se/Se or Se/se) gene. We identified a para-Bombay phenotype with two novel mutations in the FUT1 gene and homozygous mutated FUT2 (se /se ) genes.

Study Design And Methods: Red blood cell phenotype was detected by using a standard serologic technique. The entire coding regions of the FUT1 and FUT2 genes were amplified and direct sequenced using genomic DNA.

Results: No ABH substance was detected on the surface of the proband's red blood cells. Anti-A, anti-B, and anti-H were identified in serum. Genetic studies indicated that the proband's ABO genotyping was A102/O01 and that the FUT2 phenotype was se /se . The sample was homozygous for two FUT1 mutations: c.958insG and c.961G > A.

Conclusion: Two novel FUT1 mutations have been identified in the proband's FUT1 gene. The insertion mutation in the FUT1 that caused a shift of the open reading frame and formed a termination codon early at Amino Acid Position 334 may be the main reason for H deficiency in this case.
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http://dx.doi.org/10.1111/trf.13879DOI Listing
January 2017

Regulation of myocardial stromal cell-derived factor 1α/CXCL12 by tumor necrosis factor signaling.

J Surg Res 2017 01 31;207:155-163. Epub 2016 Aug 31.

Division of Cardiovascular and Thoracic Surgery, Department of Surgery, Indiana University School of Medicine, Indianapolis, Indiana.

Background: Global myocardial ischemia-reperfusion (I/R) occurs during cardiac operations. This I/R injury leads to increased production of tumor necrosis factor α (TNF) instantly and upregulated expression of stromal cell-derived factor 1 α (SDF-1). On the basis of the published data from our laboratory and other groups, locally produced TNF contributes to cardiac dysfunction mainly via binding to its receptor (tumor necrosis factor receptor 1 [TNFR1]), whereas ischemia-induced myocardial SDF-1 mediates cardioprotection. Although TNF has been shown to work as an upstream initiator for induction of other cytokines and chemokines, there is no information regarding the interaction among TNF, TNFRs, and myocardial SDF-1 expression. In this study, given that TNF downregulated SDF-1 in vascular endothelial cells, we therefore hypothesized that TNF would have a negative effect on myocardial SDF-1 production, which is attributable to TNFR-initiated actions.

Methods: Using a Langendorff model, isolated male mouse hearts were infused with TNF for 45 min. Male adult mouse hearts from wild type, TNFR1 knockout (TNFR1KO), TNFR2KO, and TNFR1/2KO were subjected to global I/R. H9c2 cells with small interfering RNA transfection were used as an in vitro model. The levels of SDF-1 (protein and messenger RNA) were detected by enzyme-linked immunosorbent assay and quantitative reverse transcription-polymerase chain reaction . Protein kinases of IκB (nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor α) and c-jun N-terminal kinase were also determined using Western blot assay.

Results: TNF infusion downregulated myocardial SDF-1 production in a dose-dependent manner in the hearts. In addition, using TNF significantly decreased SDF-1 expression in cardiomyoblasts (H9c2 cells), which was associated with reduced IκB level. Knockdown of TNFR1 or TNFR2 by small interfering RNAs neutralized TNF-suppressed SDF-1 in H9c2 cells. Furthermore, deletion of TNFR1/2 or TNFR2 increased SDF-1 production in the hearts after I/R.

Conclusions: Our study represents the initial evidence showing that TNF plays an inhibitory role in modulating myocardial SDF-1 production and blockade of TNF signaling by ablation of TNFR1 and TNFR2 genes increased SDF-1 expression in the heart. These data expand on TNF signaling-initiated mechanisms in myocardium, which may lend a more complete understanding of SDF-1 and TNFR-derived actions in hopes of advancing ischemic heart injury treatments.
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http://dx.doi.org/10.1016/j.jss.2016.08.073DOI Listing
January 2017

Unexpected red blood cell antibody distributions in Chinese people by a systematic literature review.

Transfusion 2016 Apr 6;56(4):975-9. Epub 2015 Dec 6.

Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, P.R. China.

Background: Unexpected antibodies are frequently associated with hemolytic disease of the fetus and newborn (HDFN) and hemolytic transfusion reactions (HTRs), and screening for those unexpected antibodies is critical for the safety and effectiveness of transfusion. Different populations differ in the prevalence of significant antibodies and also the low-frequency red blood cell (RBC) antigens. In China, antibody screening has been a common practice for pretransfusion testing for more than 10 years. However, there has been no national guidelines regarding the minimum antigens on the screening cells, thus making the antibody screening tests unsatisfactory.

Study Design And Methods: By a literature review, we systematically searched major databases from their inception to 2014, for publications regarding unexpected RBC antibodies, HTRs, and HDFNs in Chinese people, to explore the frequencies and clinical significance of unexpected RBC antibodies.

Results: The overall prevalence of unexpected antibodies was approximately 0.2% (14,095/6,102,361). A total of 2241 patients suffered HTRs (693 patients) or HDFN (1548 patients). Among all the reported antibodies, antibodies of the Rh blood group system were the most, followed by MNS, Lewis, Kidd, Duffy, Diego, and Kell systems. Several rare but clinically significant unexpected antibodies were reported: four anti-Le(x) , three anti-Lu(a) , one anti-Tj(a) , and one anti-Hm .

Conclusion: All the commonly seen RBC antibodies are reported in Chinese people. However, anti-K is very rare. Besides, anti-Mur, anti-Di(a) are clinically significant. We propose that in China, the antigens on the antibody screening cells should include those recommended by the AABB and British Committee for Standards in Haematology, as well as Di(a) and Mur antigens.
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http://dx.doi.org/10.1111/trf.13430DOI Listing
April 2016
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