Publications by authors named "Chunsheng Wang"

441 Publications

Identification of Differently Expressed mRNAs in Atherosclerosis Reveals CDK6 Is Regulated by circHIPK3/miR-637 Axis and Promotes Cell Growth in Human Vascular Smooth Muscle Cells.

Front Genet 2021 15;12:596169. Epub 2021 Feb 15.

Department of Cardiac Surgery, Zhongshan Hospital, Fudan University, Shanghai, China.

Atherosclerosis is the leading cause of heart disease and stroke, and one of the leading causes of death and disability worldwide. The phenotypic transformation of vascular smooth muscle cells (VSMCs) plays an important role in the pathological process of atherosclerosis. The present study aimed to identify differently expressed mRNAs in atherosclerosis by analyzing GSE6088 database. Our results revealed there were totally 467 increased and 490 decreased differential expressed genes (DEGs) in atherosclerosis. Bioinformatics analysis demonstrated that the DEGs substantially existed in pathways, including Glyoxylate and dicarboxylate metabolism, Tyrosine metabolism, Tryptophan metabolism, Beta-Alanine metabolism, Fatty acid biosynthesis and Starch and sucrose metabolism. Next, we constructed a protein-protein interaction (PPI) network to identify hub genes in atherosclerosis. Also, we identified CDK6 as a key regulator of atherosclerosis. In this study, we found that CDK6 knockdown suppressed HASMC and HUASMC cell proliferation. Circular RNA (CircRNA) is a non-coding RNA which is reported to have an unusual influence on tumorigenesis process and other aspects in the last few years. Previous studies showed circRNAs could act as miRNAs sponging in multiple biological processes. Bioinformatics prediction and luciferase analysis showed that CDK6 were targeted and regulated by circHIPK3/miR-637. Moreover, silencing circHIPK3 could also significantly induce the arrest and apoptosis of cell cycle. In conclusion, this study discovered the important regulatory role of circHIPK3 in the proliferation and apoptosis of VSMCs by influencing the miR-637/CDK6 axis.
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http://dx.doi.org/10.3389/fgene.2021.596169DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7917241PMC
February 2021

sp. nov. and report of (Maldanidae, Annelida) from the China Seas.

PeerJ 2021 18;9:e10608. Epub 2021 Feb 18.

Key Laboratory of Marine Ecosystem Dynamics, Second Institute of Oceanography, Ministry of Natural Resources, Hangzhou, China.

Polychaete species are widely distributed throughout Indo-Pacific and European waters. We collected specimens from the China Seas to report on sp. n. and (Izuka, 1902) in greater detail. Geographic analysis of the potential distribution areas of indicates that it may be found in most coastal areas of China. The newly discovered species, and , have an overlapping distribution in the northern South China Sea. sp. n. is characterized by a crenulated cephalic rim, complete collar on chaetiger 1, a packet-shaped anal funnel, and a spirally-fringed notochaetae with spiral pectinate bands imbricated over the main shaft. Our study provides a taxonomic key to all species of .
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http://dx.doi.org/10.7717/peerj.10608DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7897410PMC
February 2021

Water-Salt Oligomers Enable Supersoluble Electrolytes for High-Performance Aqueous Batteries.

Adv Mater 2021 Feb 26:e2007470. Epub 2021 Feb 26.

MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, 38 Zheda Road, Hangzhou, 310027, China.

Aqueous rechargeable batteries are highly safe, low-cost, and environmentally friendly, but restricted by low energy density. One of the most efficient solutions is to improve the concentration of the aqueous electrolytes. However, each salt is limited by its physical solubility, generally below 21-32 mol kg (m). Here, a ZnCl /ZnBr /Zn(OAc) aqueous electrolyte with a record super-solubility up to 75 m is reported, which breaks through the physical solubility limit. This is attributed to the formation of acetate-capped water-salt oligomers bridged by Br /Cl -H and Br /Cl /O-Zn interactions. Mass spectrometry indicates that acetate anions containing nonpolarized protons prohibit the overgrowth and precipitation of ionic oligomers. The polymer-like glass transition temperature of such inorganic electrolytes is found at ≈-70 to -60 °C, without the observation of peaks for salt-crystallization and water-freezing from 40 to -80 °C. This supersoluble electrolyte enables high-performance aqueous dual-ion batteries that exhibit a reversible capacity of 605.7 mAh g , corresponding to an energy density of 908.5 Wh kg , with a coulombic efficiency of 98.07%. In situ X-ray diffraction and Raman technologies reveal that such high ionic concentrations of the supersoluble electrolyte enable a stage-1 intercalation of bromine into macroscopically assembled graphene cathode.
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http://dx.doi.org/10.1002/adma.202007470DOI Listing
February 2021

Functionalized Phosphonium Cations Enable Zn Metal Reversibility in Aqueous Electrolytes.

Angew Chem Int Ed Engl 2021 Feb 12. Epub 2021 Feb 12.

U.S. Army Research Laboratory, Power and Energy Division, U.S. Army Research Laboratory, Adelphi, MD 20783, 20783, Adelphi, UNITED STATES.

Aqueous rechargeable zinc metal batteries promise attractive advantages including safety, high volumetric energy density and low cost; however, such benefits cannot be unlocked unless Zn reversibility meets stringent commercial viability. Herein, we report remarkable improvements on Zn reversibility in aqueous electrolytes when phosphonium-based cations are used to reshape interfacial structures and interphasial chemistries, particularly when their ligands contain ether linkage. This novel aqueous electrolyte supports unprecedented Zn reversibility by showing dendrite-free Zn plating/stripping for over 6400 h at 0.5 mA cm -2 , or over 280 h at 2.5 mA cm -2 , with coulombic efficiency above 99% even with 20% Zn utilization per-cycle. Excellent full cell performance is demonstrated with Na 2 V 6 O 16 ·1.63H 2 O cathode, which cycles for 2000 times at 300 mA g -1 . The microscopic characterization and modeling identify the mechanism of unique interphase chemistry from phosphonium and its functionalities as the key factors responsible for dictating reversible Zn chemistry .
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http://dx.doi.org/10.1002/anie.202017020DOI Listing
February 2021

Identification of Two Novel Linear Neutralizing Epitopes within the Hexon Protein of Canine Adenovirus Using Monoclonal Antibodies.

Vaccines (Basel) 2021 Feb 8;9(2). Epub 2021 Feb 8.

State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China.

Canine adenovirus (CAdV) has a high prevalence in canine populations. High affinity neutralizing antibodies against conserved epitopes can provide protective immunity against CAdV and protect against future outbreaks. In this study, we identified two CAdV-2-specific neutralizing monoclonal antibodies (mAbs), 2C1 and 7D7, which recognized two linear-dependent epitopes. MAb 2C1 potently neutralized CAdV-2 with a 50% neutralization titer (NT50) of 4096, and mAb 7D7 partially neutralized CAdV-2 with a 50% NT50 of 64. Immunoprecipitation, Western blot and protein spectral analysis indicated that both neutralizing mAbs recognized the hexon protein (Hex) of CAdV-2. Through a 12-mer random peptide phage display and synthetic peptides analysis, we finely mapped the neutralizing epitopes to two 10-amino acid (aa) peptides within the CAdV Hex: RIKQRETPAL located on the surface region; and PESYKDRMYS located in the inner region of the expected 3D structure of trimeric Hex. Importantly, the two epitopes are highly conserved among all CAdV isolates by sequence alignment analysis. Thus, these results provide insights into the interaction between virus and mAbs at the aa level and may have potential applications in the development of novel therapeutic or epitope-based vaccines, antibody therapeutics and a diagnostic method suitable for the rapid detection of all CAdVs.
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http://dx.doi.org/10.3390/vaccines9020135DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7914820PMC
February 2021

Integrative Identification of Hub Genes Associated With Immune Cells in Atrial Fibrillation Using Weighted Gene Correlation Network Analysis.

Front Cardiovasc Med 2020 21;7:631775. Epub 2021 Jan 21.

Department of Cardiovascular Surgery, Zhongshan Hospital, Fudan University, Shanghai, China.

Atrial fibrillation (AF) is the most common tachyarrhythmia in the clinic, leading to high morbidity and mortality. Although many studies on AF have been conducted, the molecular mechanism of AF has not been fully elucidated. This study was designed to explore the molecular mechanism of AF using integrative bioinformatics analysis and provide new insights into the pathophysiology of AF. The GSE115574 dataset was downloaded, and Cibersort was applied to estimate the relative expression of 22 kinds of immune cells. Differentially expressed genes (DEGs) were identified through the limma package in R language. Weighted gene correlation network analysis (WGCNA) was performed to cluster DEGs into different modules and explore relationships between modules and immune cell types. Functional enrichment analysis was performed on DEGs in the significant module, and hub genes were identified based on the protein-protein interaction (PPI) network. Hub genes were then verified using quantitative real-time polymerase chain reaction (qRT-PCR). A total of 2,350 DEGs were identified and clustered into eleven modules using WGCNA. The magenta module with 246 genes was identified as the key module associated with M1 macrophages with the highest correlation coefficient. Three hub genes (, and ) were identified. The results verified using three other datasets and qRT-PCR demonstrated that the expression levels of these three genes in patients with AF were significantly higher than those in patients with SR, which were consistent with the bioinformatic analysis. Three novel genes identified using comprehensive bioinformatics analysis may play crucial roles in the pathophysiological mechanism in AF, which provide potential therapeutic targets and new insights into the treatment and early detection of AF.
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http://dx.doi.org/10.3389/fcvm.2020.631775DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7859264PMC
January 2021

Hemodilution is associated with underestimation of serum creatinine in cardiac surgery patients: a retrospective analysis.

BMC Cardiovasc Disord 2021 Jan 31;21(1):61. Epub 2021 Jan 31.

Department of Nephrology, Zhongshan Hospital, Shanghai Medical College, Fudan University, 180 Fenglin Road, Shanghai, 200032, China.

Background: Fluid overload is related to the development and prognosis of cardiac surgery-associated acute kidney injury (CSA-AKI). The study is to investigate the influence of serum creatinine (SCr) corrected by fluid balance on the prognosis of patients with cardiac surgery.

Methods: A retrospective study was conducted in 1334 patients who underwent elective cardiac surgery from January 1 to December 31, 2015. Kidney Disease: Improving Global Outcomes (KDIGO) criteria for AKI were applied to identify CSA-AKI. SCr was measured every 24 h during ICU period and was accordingly adjusted for cumulative fluid balance. Changes in SCr, defined as ∆Crea, were determined by difference between before and after adjustment for cumulative fluid balance. All patients were then divided into three groups: underestimation group (∆Crea ≥ P), normal group (P < ∆Crea < P) and overestimation group (∆Crea ≤ P).

Results: The incidence of AKI increased from 29.5% to 31.8% after adjustment for fluid balance. Patients in underestimation group showed prolonged length of ICU stay compared with normal group and overestimation group (3.2 [1.0-4.0] vs 2.1 [1.0-3.0] d, P < 0.001; 3.2  [1.0-4.0] vs 2.3 [1.0-3.0] d, P < 0.001). Length of hospital stay and mechanical ventilation dependent days in underestimation group were significantly longer than normal group (P < 0.001). Multivariate analysis showed age, baseline SCr and left ventricular ejection fraction were independently associated with underestimation of creatinine.

Conclusions: Cumulative fluid balance after cardiac surgery disturbs accurate measurement of serum creatinine. Patients with underestimation of SCr were associated with poor prognosis.
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http://dx.doi.org/10.1186/s12872-021-01879-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7849106PMC
January 2021

Identification of LiH and nanocrystalline LiF in the solid-electrolyte interphase of lithium metal anodes.

Nat Nanotechnol 2021 Jan 28. Epub 2021 Jan 28.

Chemistry Division, Brookhaven National Laboratory, Upton, NY, USA.

A comprehensive understanding of the solid-electrolyte interphase (SEI) composition is crucial to developing high-energy batteries based on lithium metal anodes. A particularly contentious issue concerns the presence of LiH in the SEI. Here we report on the use of synchrotron-based X-ray diffraction and pair distribution function analysis to identify and differentiate two elusive components, LiH and LiF, in the SEI of lithium metal anodes. LiH is identified as a component of the SEI in high abundance, and the possibility of its misidentification as LiF in the literature is discussed. LiF in the SEI is found to have different structural features from LiF in the bulk phase, including a larger lattice parameter and a smaller grain size (<3 nm). These characteristics favour Li transport and explain why an ionic insulator, like LiF, has been found to be a favoured component for the SEI. Finally, pair distribution function analysis reveals key amorphous components in the SEI.
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http://dx.doi.org/10.1038/s41565-020-00845-5DOI Listing
January 2021

Giant cardiac myxoma presenting with exertional angina: Coronary steal due to neovascularization?

J Card Surg 2021 Mar 21;36(3):1134-1135. Epub 2021 Jan 21.

Department of Cardiac Surgery, Zhongshan Hospital Fudan University, Shanghai, China.

Myxoma is the most common benign tumor of the heart. Most patients present with no symptoms, only a few patients present with exertional dyspnea and stroke. We introduce this rare case presenting with exertional angina, which was caused by coronary steal due to neovascularization, proved by coronary angiography and cardiac stress testing.
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http://dx.doi.org/10.1111/jocs.15366DOI Listing
March 2021

Complete mitochondrial genome sequence of the genus (Malacostraca: Brachyura: Bythograeidae) and its phylogenetic analysis.

Mitochondrial DNA B Resour 2020 Oct 5;5(3):3487-3488. Epub 2020 Oct 5.

Key Laboratory of Marine Ecosystem Dynamics, Second Institute of Oceanography, Ministry of Natural Resources, Hangzhou, PR China.

species are restricted to hydrothermal vents and are typically considered to be omnivorous predators in vent communities. Here we present the complete mitochondrial genome of sp. which was collected from Carlsberg Ridge, the mid-ocean ridge in the northwestern Indian Ocean. The genome is 15,584 bp in length with a 68.11% AT content. It contains 13 protein-coding genes (PCGs), 2 ribosomal RNA genes, and 22 transfer RNA genes. Phylogenetic analysis shows that the present species is closest to . This study contributes to further phylogenetic analysis within Eubrachyura.
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http://dx.doi.org/10.1080/23802359.2020.1821823DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7782158PMC
October 2020

Postoperative hypothermia after total aortic arch replacement in acute type A aortic dissection-multivariate analysis and risk identification for postoperative hypothermia occurrence.

J Thorac Dis 2020 Dec;12(12):7089-7096

Department of Cardiovascular Surgery, Zhongshan Hospital, Shanghai Cardiovascular Institution, Fudan University, Shanghai, China.

Background: Postoperative hypothermia (PH) is a common physiological abnormality associated with increased morbidity and mortality after non-cardiac surgery. The incidence, risk factors of PH and its impact on early outcomes after total aortic arch replacement are not clear.

Methods: We conducted a retrospective cohort study in patients with acute type A aortic dissection who underwent total arch replacement from January 2013 to December 2016 at our institution. Basic variables, procedural and postoperative early outcomes were collected. Univariate and multivariate statistical analysis were performed for statistical interpretation. The early outcomes were compared between patients with or without PH.

Results: A total of 300 patients (age 53.8±11.5 years, female 63, 21.0%) with acute type A aortic dissection underwent total arch replacement. Forty-four patients (14.7%) developed PH. The independent risk factors of PH are age and the intraoperative lowest bladder temperature. There is no significant difference in major postoperative morbidity and mortality between patients with or without PH.

Conclusions: The incidence of PH after total arch replacement in acute type A aortic dissection is relatively low. The independent risk factors of PH in this population include age and the intraoperative lowest bladder temperature. With comprehensive rewarming strategy upon arrival at the ICU, the PH is easy to be corrected, and the adverse effect of transient PH on early outcomes after arch surgery is minimal.
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http://dx.doi.org/10.21037/jtd-20-1709DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7797820PMC
December 2020

Combined prognostic value of the SUVmax derived from FDG-PET and the lymphocyte-monocyte ratio in patients with stage IIIB-IV non-small cell lung cancer receiving chemotherapy.

BMC Cancer 2021 Jan 14;21(1):66. Epub 2021 Jan 14.

Department of Radiation Oncology, Yantai Yuhuangding Hospital, 20 Yudong Road, Yantai, 264000, Shandong, People's Republic of China.

Background: We evaluated the prognostic potential of tumor F-fluorodeoxyglucose (FDG) uptake derived from positron emission tomography (PET) and known inflammatory hematological markers, both individually and in combination, for chemosensitivity and survival in patients with stage IIIB-IV non-small cell lung cancer (NSCLC) receiving first-line chemotherapy.

Methods: A total of 149 patients with stage IIIB and IV NSCLC (based on TNM 7th edition) were retrospectively reviewed. Maximum standardized uptake value (SUVmax) were used to quantitatively assess FDG uptake. The lymphocyte-monocyte ratio (LMR), neutrophil-lymphocyte ratio (NLR) and platelet-lymphocyte ratio (PLR) were selected as hematological markers. Receiver operating characteristic (ROC) curves were constructed for the determination of optimal cut-off values to predict chemotherapeutic response.

Results: Patients with SUVmax > 11.6 or LMR ≤3.73 exhibited a significantly lower objective response rate (ORR) to chemotherapy (p < 0.001 and p < 0.001). Through multivariable logistic regression analysis, both the SUVmax and LMR were identified as independent predictive factors for chemotherapeutic response (p = 0.001 and p < 0.001). Furthermore, a multivariable Cox proportional hazard model identified a high SUVmax (> 11.6) and low LMR (≤3.73) as independent predictors of poor PFS (p < 0.001 and p = 0.025) and OS (p < 0.001 and p = 0.032). A novel score system was constructed based on the SUVmax and LMR (SUV_LMR score), and patients were stratified into three subgroups. The patients with a score of 0 had a significantly higher ORR (88.9%) than did those with a score of 1 (59.6%) and score of 2 (25.0%) (p < 0.001). Moreover, multivariable Cox analysis further identified the SUV_LMR score as an independent prognostic factor for PFS (p < 0.001) and OS (p < 0.001).

Conclusions: Pre-treatment SUVmax and LMR were not only predictive factors for chemotherapeutic response but also independent prognostic factors of survival in stage IIIB-IV NSCLC. Moreover, the SUV_LMR score, which is based on primary tumor metabolic activity and the systemic inflammatory response, might provide a promising tool to predict chemosensitivity, recurrence and survival of advanced NSCLC.
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http://dx.doi.org/10.1186/s12885-021-07784-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7809816PMC
January 2021

Electrolytes and Interphases in Potassium Ion Batteries.

Adv Mater 2021 Feb 6;33(7):e2003741. Epub 2021 Jan 6.

School of Materials Science and Engineering, Key Laboratory of Advanced Ceramics and Machining Technology (Ministry of Education) and Tianjin Key Laboratory of Composite and Functional Materials, Tianjin University, Tianjin, 300072, China.

Potassium ion batteries (PIBs) are recognized as one promising candidate for future energy storage devices due to their merits of cost-effectiveness, high-voltage, and high-power operation. Many efforts have been devoted to the development of electrode materials and the progress has been well summarized in recent review papers. However, in addition to electrode materials, electrolytes also play a key role in determining the cell performance. Here, the research progress of electrolytes in PIBs is summarized, including organic liquid electrolytes, ionic liquid electrolytes, solid-state electrolytes and aqueous electrolytes, and the engineering of the electrode/electrolyte interfaces is also thoroughly discussed. This Progress Report provides a comprehensive guidance on the design of electrolyte systems for development of high performance PIBs.
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http://dx.doi.org/10.1002/adma.202003741DOI Listing
February 2021

Evaluation of Knee Kinematics and Moments during Active Deep Flexion Activity after Oxford Mobile-Bearing Medial UKA-A Two-Year Follow-Up Study.

J Knee Surg 2021 Jan 5. Epub 2021 Jan 5.

Department of Orthopaedics, The Second Affiliated Hospital of Xi'an Jiaotong University, Shaanxi Province, P.R. China.

Few studies have assessed knee range of motion (ROM) and moments of patients with mobile-bearing unicompartmental knee arthroplasty (MB UKA) during active deep flexion activities. We analyze knee kinematic and kinetic parameters during postoperative squatting-standing activity, aiming to evaluate the efficacy of MB UKA and postoperative rehabilitation progress. This was a clinical cohort study. We followed up with 37 patients diagnosed with medial knee osteoarthritis (OA) with primary UKA. After screening 31 patients were recruited to take gait tests. Squatting-standing activities were performed under the test of 10-camera motion analysis system and force plates preoperatively at different stages after UKA (12, 18, and 24 months). The average duration of follow-up was 24.4 months (from 22.8 to 26.7 months). Hip-knee-ankle angle improved significantly compared with pre-UKA as well as scores of American Knee Society Score, numeric rating scale, ORS, and Western Ontario and McMasters. University Osteoarthritis Index. About 83.6% (31/37) of follow-up patients completed squatting-standing activity independently. At 1-year follow-up, peak varus angle (20.6 ± 2.8 degrees), internal rotation angle (13.6 ± 1.8 degrees), extensor moment (1.44 ± 0.04N*m/kg), and internal rotator moment (0.02 ± 0.005N*m/kg) of UKA knees were inferior to contralateral knees. Peak adductor moment (0.76 ± 0.05N*m/kg) was superior to contralateral knees. At 2-year follow-up, peak flexion angle (125.0 ± 2.8 degrees) showed a growing trend meanwhile extensor (1.70 ± 0.03N*m/kg) and adductor (0.68 ± 0.06 N*m/kg) moment closely resembled those of the contralateral knee. MB UKA could alleviate the affected knee mainly in flexion-extension ROM and moment meanwhile did not affect the biomechanical indicators of healthy limbs. OA knees in the early postoperative period showed decreased extensor moment and increased adductor moment during active deep flexion activity. Better ROM and relatively more natural extensor and adductor moment of UKA knee with rehabilitation time increasing may predict ideal rehabilitation outcome in the medium or longer term.
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http://dx.doi.org/10.1055/s-0040-1721033DOI Listing
January 2021

A rechargeable zinc-air battery based on zinc peroxide chemistry.

Science 2021 01;371(6524):46-51

MEET Battery Research Center, Institute of Physical Chemistry, University of Münster, Münster, Germany.

Rechargeable alkaline zinc-air batteries promise high energy density and safety but suffer from the sluggish 4 electron (e)/oxygen (O) chemistry that requires participation of water and from the electrochemical irreversibility originating from parasitic reactions caused by caustic electrolytes and atmospheric carbon dioxide. Here, we report a zinc-O/zinc peroxide (ZnO) chemistry that proceeds through a 2e/O process in nonalkaline aqueous electrolytes, which enables highly reversible redox reactions in zinc-air batteries. This ZnO chemistry was made possible by a water-poor and zinc ion (Zn)-rich inner Helmholtz layer on the air cathode caused by the hydrophobic trifluoromethanesulfonate anions. The nonalkaline zinc-air battery thus constructed not only tolerates stable operations in ambient air but also exhibits substantially better reversibility than its alkaline counterpart.
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http://dx.doi.org/10.1126/science.abb9554DOI Listing
January 2021

Characteristics and Surgical Results of Patients with Hypertrophic Obstructive Cardiomyopathy without Intrinsic Mitral Valve Diseases Undergoing Mitral Subvalvular Procedures during Myectomy.

Cardiol Res Pract 2020 9;2020:8875405. Epub 2020 Dec 9.

Shanghai Institute of Cardiovascular Diseases, 1609 Xietu Road, Shanghai 200032, China.

Background: Mitral subvalvular procedures have acquired a major role during hypertrophic obstructive cardiomyopathy (HOCM) surgery. However, few studies have focused on characterizing the clinical feature of HOCM patients without intrinsic mitral valve (MV) diseases undergoing mitral subvalvular procedures in addition to myectomy. Additionally, scant data about the results of mitral subvalvular procedures during HOCM surgery are available. This single-center study aims to characterize the clinical feature and surgical results of HOCM patients without intrinsic MV diseases undergoing mitral subvalvular procedures in addition to myectomy in comparison with those receiving myectomy alone.

Methods: Among 181 eligible patients, 50 (27.6%) patients undergoing myectomy plus mitral subvalvular procedures were entered into the combined group, and the remaining 131 patients receiving myectomy alone were included in the alone group. Baseline and surgical characteristics were investigated, and surgical results were compared.

Results: Comparatively, the combined group was younger (52.9 ± 11.2 years vs. 56.8 ± 11.8 years, =0.045) and had a better New York Heart Association (NYHA) class (=0.034) and less septal hypertrophy (16.4 ± 2.3 mm vs. 18.5 ± 3.2 mm, < 0.001). Septal thickness was independently associated with combined procedures in multivariable logistic regression analysis (OR = 0.887, 95% CI 0.612-0.917). No surgical death or iatrogenic septal perforation occurred in the combined group. Two (6.5%) patients in the combined group developed complete atrioventricular block and required permanent pacemaker implantation. During a median follow-up of 10 months, no deaths or reoperations were observed with the symptom of relief and NYHA class I or II in either group. Patients in the combined group as compared to the alone group had lower outflow tract gradients and a lower incidence of residual systolic anterior motion (SAM) syndrome.

Conclusions: For HOCM patients without intrinsic MV diseases who are scheduled for surgery, a less pronounced septal hypertrophy may be closely associated with myectomy with concomitant mitral subvalvular procedures instead of myectomy alone. Mitral subvalvular procedures during myectomy are safe and allow the reduction of outflow tract gradients and freedom from SAM more effectively in comparison with myectomy alone.
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http://dx.doi.org/10.1155/2020/8875405DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7744219PMC
December 2020

The mitochondrial genome of the bone-eating worm (Annelida, Siboglinidae).

Mitochondrial DNA B Resour 2020 1;5(3):2267-2268. Epub 2020 Jun 1.

Key Laboratory of Marine Ecosystem Dynamics, Second Institute of Oceanography, Ministry of Natural Resources, Hangzhou, China.

(Annelida, Siboglinidae)uses heterotrophic bacteria to feed onvertebrate carcasses and is currently found in the Pacific, Antarctic and Indian Ocean.Here, we report its nearly complete mitochondrial genomes assembled for 2 individuals, one from the East Pacific and the other from the Southwest Indian Ocean. Recoveredmitogenomes were 15591 and 15972 bp in length, with both consisting of 37 typical metazoan mitochondrial genes. All genes were transcribed from the same strand, and arranged in the same order as the other siboglinids, revealing conserved gene arrangement withinSiboglinidae. Phylogeneticanalysis of 13 protein coding genes confirms the placement of sister to the Vestimentifera+ clade.
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http://dx.doi.org/10.1080/23802359.2020.1772680DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7510606PMC
June 2020

Complete mitochondrial genome of (Sessilia: Tetraclitidae) from China and phylogeny within Cirripedia.

Mitochondrial DNA B Resour 2020 20;5(3):2121-2123. Epub 2020 May 20.

Key Laboratory of Marine Ecosystem Dynamics, Second Institute of Oceanography, Ministry of Natural Resources, Hangzhou, China.

Here we present the complete mitochondrial genome of , which is 15,191 bp in length with 67.20% AT content. It contains 13 protein-coding genes, 2 ribosomal-RNA genes and 22 transfer-RNA genes. All PCGs except nad4l in start with ATN, and terminated with a complete stop codon, except nad3. Phylogenetic analysis based on mitochondrial PCGs shows that is clustered with into a branch (BP = 100). Our result is consistent with previous reports that genus and family are not monophyletic. This study contributes to further phylogenetic analysis within Cirripedia.
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http://dx.doi.org/10.1080/23802359.2020.1765705DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7510661PMC
May 2020

The first mitochondrial genome of (Sessilia: Balanidae) from China: phylogeny within Cirripedia based on mitochondrial genes.

Mitochondrial DNA B Resour 2019 Nov 13;4(2):4016-4018. Epub 2019 Nov 13.

Marine and Fisheries Research Institute, Zhejiang Ocean University, Zhoushan, PR China.

Here we present the complete mitochondrial genome of . The genome is 15,107 bp in length with a 67.35% AT content. It contains 13 protein-coding genes (PCGs), 2 rRNAs genes, and 22 tRNAs. Both rRNAs are encoded on the light strand, as in the other crustacean and barnacle mitochondrial genomes. Besides five tRNAs are encoded on the light strand (nad1, trnV, trnL1, trnC, trnQ, and trnK). Only one PCG is encoded on the light strand (nad1), whereas the other 12 PCGs are located on the heavy strand, which is consistent with . . Phylogenetic analysis based on mitochondrial PCGs shows that . is clustered with . into a branch (BP = 100), and the group with . with high support. This study contributes to further phylogenetic analysis within Cirripedia.
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http://dx.doi.org/10.1080/23802359.2019.1688104DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7707791PMC
November 2019

Phase I Trial of Fourth-Generation Anti-CD19 Chimeric Antigen Receptor T Cells Against Relapsed or Refractory B Cell Non-Hodgkin Lymphomas.

Front Immunol 2020 27;11:564099. Epub 2020 Nov 27.

Department of Hematology, Zhujiang Hospital, Southern Medical University, Guangzhou, China.

Background: The administration of second- or third-generation anti-CD19 chimeric antigen receptor (CAR) T cells has remarkably improved the survival of patients with relapsed or refractory B cell malignancies. However, there are limited clinical results from fourth-generation CAR-T cell therapy, and the factors affecting response rate and survival have not been fully determined.

Methods: Lymphoma patients with progression or relapse after intensive treatments, including hematopoietic stem cell transplantation, and life expectancy >2 months were enrolled in the study. Peripheral lymphocytes were collected through apheresis, and magnetically selected T cells were lentivirally transduced with a 4th-generation CAR featuring an anti-CD19 CAR and the iCasp9 suicide switch (4SCAR19). The patients received 4SCAR19 T cell infusion after approximately seven days of expansion and a conditioning regimen comprising cyclophosphamide/fludarabine. The efficacy, safety, and risk factors were evaluated.

Results: A total of 21 patients with relapsed/refractory B cell non-Hodgkin lymphoma were enrolled and received 4SCAR19 T cell infusions at a median dose of 8.9×10 CAR-T cells/kg. The overall response rate was 67% [95% confidence interval (CI), 43 to 85], with 43% of patients achieving a complete response and 24% having a partial response. The overall and complete response rates were 58 and 33% in the diffuse large B-cell lymphoma (DLBCL) group and 78 and 56% in the non-DLBCL group, respectively. The median overall survival was 23.8 months (95% CI, not reached), with a median follow-up of 13.7 months. Factors affecting overall survival were International Prognostic Index (IPI), disease type, and remission status after CAR-T cell treatment. The most common adverse events of grade 3 or 4 during treatment were neutropenia (76%), leukopenia (71%), and thrombocytopenia (29%). The incidence of cytokine release syndrome (CRS) was 14%, and all cases were grade 1. One patient developed grade 3 neurotoxicity. No deaths were attributed to infusion of 4SCAR19 T cells, CRS, or neurotoxicity.

Conclusions: In this study, patients with relapsed or refractory B cell non-Hodgkin's lymphoma who received 4SCAR19 T cell therapy had durable responses and few of adverse events. The IPI model is suitable for evaluating the prognosis of patients receiving CAR-T cell therapy.

Trial Registration: Chinese Clinical Trial Registry (http://www.chictr.org.cn): ChiCTR-OOC-16007779.
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http://dx.doi.org/10.3389/fimmu.2020.564099DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7731732PMC
November 2020

The impact of minimally invasive technique on the outcomes of isolated tricuspid valve surgery.

Eur Heart J 2020 Dec 12. Epub 2020 Dec 12.

Department of Cardiovascular Surgery, Zhongshan Hospital, Fudan University, No.180 Fenglin Rd, Xuhui District, Shanghai 200032, China.

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http://dx.doi.org/10.1093/eurheartj/ehaa966DOI Listing
December 2020

Mitral valve repair and surgical ablation for atrial functional mitral regurgitation.

Ann Transl Med 2020 Nov;8(21):1420

Department of Cardiac Surgery, Zhongshan Hospital, Fudan University, Shanghai, China.

Background: This observational study aimed to share our experience in the surgical management of atrial functional mitral regurgitation (AFMR).

Methods: We retrospectively identified 82 AFMR patients (63.6±7.7 years) from June 2008 to November 2018 at our institution. Of these patients, 72.0% of them were classified as NYHA functional class III/IV, and all of them had persistent AF. All patients underwent mitral valve (MV) repair, and 52 (63.4%) received concomitant surgical ablation (SA). Patients were followed up for 26.1±27.6 months, and postoperative mitral regurgitation (MR) was assessed by echocardiography.

Results: There was no in-hospital mortality. The overall 1-year and 3-year survival rates were 97.5% and 92.9%, respectively, and 96.1% of patients recovered to NYHA functional class I/II at the latest follow-up. The left atrium (LA) diameter (P<0.001), left ventricular (LV) end-diastolic diameter (P<0.001), LV end-systolic diameter (LVESD) (P<0.001) and pulmonary artery pressure (P=0.006) significantly decreased postoperatively. The overall 1-year and 3-year freedom from recurrent MR rates were 94.3% and 65.3%, respectively, and a significant difference was found between the SA group and the non-SA group (93.8% and 93.8% . 95.5% and 44.2%, P=0.035). In a subgroup analysis, this significant difference was only found in the small LA group (≤60 mm).

Conclusions: Our results suggest that MV repair for AFMR is safe and effective. It improves heart failure symptoms and results in reverse-remodeling of both the LA and LV. Concomitant SA might benefit patients in terms of recurrent MR, especially in the small LA group (≤60 mm).
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http://dx.doi.org/10.21037/atm-20-2958DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7723636PMC
November 2020

A new species of glass sponge (Hexactinellida: Sceptrulophora: Uncinateridae) from the Weijia Seamount in the northwestern Pacific Ocean.

Zootaxa 2020 Nov 13;4878(2):zootaxa.4878.2.6. Epub 2020 Nov 13.

Key Laboratory of Marine Ecosystem Dynamics, Second Institute of Oceanography, Ministry of Natural Resources, Hangzhou, 310012, P. R. China Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai, 519000, P. R. China.

In this article we report on a hexactinellid sponge new to science, Tretopleura weijicus sp. nov., which was collected from the Weijia Seamount in the northwestern Pacific Ocean at a depth of 1995 m. Its blade-like and branching body form, the primary choanosomal framework consisting of multiaxial longitudinal strands without synapticula, and the presence of sceptrules suggest placement within the family Uncinateridae Reiswig, 2002, which is also supported by molecular phylogenetic evidence from COI and 16S sequences. The absence of swollen dermal spurs or regular pentactins and the presence of two types of discohexasters characterize it as a new species. More specimens should be collected to revise the generic characters.
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http://dx.doi.org/10.11646/zootaxa.4878.2.6DOI Listing
November 2020

Solvation Structure Design for Aqueous Zn Metal Batteries.

J Am Chem Soc 2020 12 8;142(51):21404-21409. Epub 2020 Dec 8.

Department of Chemical and Biomolecular Engineering, University of Maryland, College Park, Maryland 20742, United States.

Aqueous Zn batteries are promising energy storage devices for large-scale energy-storage due to low cost and high energy density. However, their lifespan is limited by the water decomposition and Zn dendrite growth. Here, we suppress water reduction and Zn dendrite growth in dilute aqueous electrolyte by adding dimethyl sulfoxide (DMSO) into ZnCl-HO, in which DMSO replaces the HO in Zn solvation sheath due to a higher Gutmann donor number (29.8) of DMSO than that (18) of HO. The preferential solvation of DMSO with Zn and strong HO-DMSO interaction inhibit the decomposition of solvated HO. In addition, the decomposition of solvated DMSO forms Zn(SO)Cl(OH)·5HO, ZnSO, and ZnS enriched-solid electrolyte interphase (SEI) preventing Zn dendrite and further suppressing water decomposition. The ZnCl-HO-DMSO electrolyte enables Zn anodes in Zn||Ti half-cell to achieve a high average Coulombic efficiency of 99.5% for 400 cycles (400 h), and the Zn||MnO full cell with a low capacity ratio of Zn:MnO at 2:1 to deliver a high energy density of 212 Wh/kg (based on both cathode and anode) and maitain 95.3% of the capacity over 500 cycles at 8 C.
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http://dx.doi.org/10.1021/jacs.0c09794DOI Listing
December 2020

Retraction Note: Fibrauretine reduces ischemia/reperfusion injury via RISK/eNOS activation.

Naunyn Schmiedebergs Arch Pharmacol 2021 Mar;394(3):573

Shenzhen Longhua District Central Hospital, Shenzhen, 518000, China.

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http://dx.doi.org/10.1007/s00210-020-02027-5DOI Listing
March 2021

Learning curve of mitral valve repair: cumulative sum failure analysis from single high-volume center.

J Thorac Dis 2020 Nov;12(11):6563-6572

Department of Cardiac Surgery, Zhongshan Hospital, Fudan University, Shanghai, China.

Background: Mitral valve (MV) repair has become the gold standard for treating degenerative mitral regurgitation (MR), yet the success rate of MV repair is still low in clinical practice. While studies focused on the learning process of MV repair are scarce, fully understanding the learning curve could provide valuable information for education and the quality control of MV repair, thus benefiting patients. This observational study aimed to evaluate the learning process and performances of individual surgeon for MV repair for degenerative mitral disease using data from a single high-volume center.

Methods: Profiles of patients who underwent MV repair for degenerative MR at our institution from January 2003 to December 2016 were analyzed retrospectively. Overall and individual learning curves for the repair rate and major adverse events were calculated using sequential probability cumulative sum failure analysis. Average learning curves for major adverse events and operative time were also analyzed, by calculating the average incidence of adverse events and operative time of all operations stratified by accumulated operation numbers of individual surgeon. Altogether, we evaluated 2,482 operations performed by 14 surgeons.

Results: There was an obvious learning curve for the repair rate at the institution and individual surgeon levels. Altogether, 50 to 200 operations were needed to overcome the repair rate learning curve, yet wide variation was observed among individual surgeons. The learning process for individual surgeons became faster after the turning point in the institutional learning curve appeared. No obvious learning curve was observed at the institution or individual level for major adverse events and in-hospital mortality.

Conclusions: The number of cases required to overcome the learning curve for repair rate is substantial, although there is marked variation among surgeons. Individuals' learning curves accelerate as the institution accumulates experience. MV repair is safe in experienced high-volume center. Close monitoring is necessary when surgeons begin to practice new techniques.
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http://dx.doi.org/10.21037/jtd-20-1960DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7711428PMC
November 2020

Nano-Biomaterials for the Delivery of Therapeutic and Monitoring Cues for Aortic Diseases.

Front Bioeng Biotechnol 2020 5;8:583879. Epub 2020 Nov 5.

Department of Cardiac Surgery, Zhongshan Hospital, Fudan University, Shanghai, China.

The aorta is the largest artery in the body, so any diseases or conditions which could cause damage to the aorta would put patients at considerable and life-threatening risk. In the management of aortic diseases, the major treatments include drug therapy, endovascular treatment, and surgical treatment, which are of great danger or with a poor prognosis. The delivery of nano-biomaterials provides a potential development trend and an emerging field where we could monitor patients' conditions and responses to the nanotherapeutics. One of the putative applications of nanotechnology is ultrasensitive monitoring of cardiovascular markers by detecting and identifying aneurysms. Moreover, the use of nanosystems for targeted drug delivery can minimize the systemic side effects and enhance drug positioning and efficacy compared to conventional drug therapies. This review shows some examples of utilizing nano-biomaterials in organ and cell culture experiments and explains some developing technologies in delivering and monitoring regenerative therapeutics.
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http://dx.doi.org/10.3389/fbioe.2020.583879DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7674648PMC
November 2020

Minimally invasive valve replacement for late tricuspid regurgitation after left-sided valve surgery.

Ann Thorac Surg 2020 Nov 17. Epub 2020 Nov 17.

Department of Cardiac Surgery, Zhongshan Hospital, Fudan University, Shanghai, China; Shanghai Engineering Research Center of Heart Valve, Shanghai, China; Shanghai Institute of Cardiovascular Disease, Shanghai, China. Electronic address:

Late severe tricuspid regurgitation after left-sided valve surgery can negatively affect long-term prognosis and quality of life. Because of extensive trauma and poor right heart function, perioperative mortality after reoperation for tricuspid regurgitation historically remained high. To its end, multiple minimally invasive techniques were adopted at our center recently, including endoscopy-assist right minithoracotomy, vacuum-assist single femoral venous drainage without dissecting or snaring vena cava, direct right atriotomy through pericardium and the beating-heart technique. Moreover, the tricuspid valves were replaced with bioprostheses in majority of those patients at our center. The mid-term outcomes of minimally invasive bioprosthetic replacement were favorable.
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http://dx.doi.org/10.1016/j.athoracsur.2020.08.091DOI Listing
November 2020

Comparison of Procedural and 1-Year Clinical Results of Transcatheter Aortic Valve Implantation Using Prostheses with Different Design of Support Frame.

Int Heart J 2020 Nov 13;61(6):1196-1203. Epub 2020 Nov 13.

Department of Structural Heart Disease, Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College.

Our study aimed to investigate whether the frame design of transcatheter heart valve (THV) affects the procedural and clinical results of transcatheter aortic valve implantation (TAVI).We retrospectively reviewed 163 patients with aortic stenosis who underwent TAVI using different types of THV (Edwards SAPIEN, n = 31; Venus-A, n = 63; and J-Valve, n = 69). The procedural outcomes and follow-up results for 1-year were compared among groups.The patients who underwent TAVI using J-Valve had a higher mean transaortic pressure gradient than those using SAPIEN or Venus-A after TAVI (1-year follow-up; P = 0.017, P < 0.001, respectively), whereas no difference was observed between the patients with SAPIEN and Venus-A prosthesis (P = 0.150). The incidence of permanent pacemaker implantation was highest in patients with Venus-A (19.0%), followed by SAPIEN (9.7%), and lowest in J-Valve (4.3%) (P = 0.025). No difference was observed in the 30-day mortality rate among the groups (P = 1.000). Moreover, Kaplan-Meier survival analysis revealed that there was no significant difference in the 1-year cumulative patient survival rate among three patient cohorts (log-rank, P = 0.850).The frame design of THVs could affect the valve-related hemodynamics and the incidence of permanent pacemaker implantation in TAVI, whereas it did not influence the survival rate of TAVI patients during 1-year follow-up period. All three THVs provided a convincing short-term outcome for TAVI patients.
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http://dx.doi.org/10.1536/ihj.20-398DOI Listing
November 2020

Modeling aortic diseases using induced pluripotent stem cells.

Stem Cells Transl Med 2021 Feb 12;10(2):190-197. Epub 2020 Nov 12.

Department of Cardiac Surgery, Zhongshan Hospital, Fudan University, Shanghai, People's Republic of China.

Induced pluripotent stem cells (iPSCs) offer an effective platform for studies of human physiology and have revealed new possibilities for disease modeling at the cellular level. These cells also have the potential to be leveraged in the practice of precision medicine, including personalized drug testing. Aortic diseases result in significant morbidity and mortality and pose a global burden to healthcare. Their pathogenesis is mostly associated with functional alterations of vascular components, such as endothelial cells and vascular smooth muscle cells. Drugs that have been proven to be effective in animal models often fail to protect patients from adverse aortic events in clinical studies, provoking researchers to develop reliable in vitro models using human cells. In this review, we summarize the patient iPSC-derived aortic cells that have been utilized to model aortic diseases in vitro. In advanced models, hemodynamic factors, such as blood flow-induced shear stress and cyclic strain, have been added to the systems to replicate cellular microenvironments in the aortic wall. Examples of the utility of such factors in modeling various aortopathies, such as Marfan syndrome, Loeys-Dietz syndrome, and bicuspid aortic valve-related aortopathy, are also described. Overall, the iPSC-based in vitro cell models have shown the potential to promote the development and practice of precision medicine in the treatment of aortic diseases.
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http://dx.doi.org/10.1002/sctm.20-0322DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7848399PMC
February 2021